FORM 2
THE PATE NT ACT, 1970
(39 of 1970)
&
THE PATENT RULES, 2003
COMPLETE SPECIFICATION
(See section 10 and rule 13)
1. TITLE OF THE INVENTION
"BIOMEDICAL DEVICE FOR IMPROVED DESICCATION TOLERANCE OF GRAFTS."
2. APPLICANT
Name - Veol Medical Technologies Pvt. Ltd. Nationality - Indian Company
Address - A-747, Near Pavan Bus Stop, MIDC- Pawane, TTC Industrial Area, Koparkhairane, Navi Mumbai 400705, Maharashtra, India.
3. PREAMBLE TO THE DESCRIPTION
COMPLETE SPECIFICATION
The following specification particularly describes the invention and the manner in which it is to be performed:

CROSS-REFERENCE TO RELATED CASES
[1] This application claims priority to Indian Provisional Application No. 2611/MUM/2014 entitled "Implantation Device for Hair Transplant," filed August 13, 2014; Indian Provisional Application No. 2612/MUM/2014 entitled "Biomedical device for improving desiccation tolerance of hair follicles," filed August 13, 2014; Indian Provisional Application No. 2987/MUM/2014 entitled "Harvesting Device for Hair Transplant," filed September 18, 2014; Indian Provisional Application No. 4011/MUM/2014 entitled "Follicle Holding Tray for Hair Transplant," filed December 15, 2014; Indian Provisional Application No. 4012/MUM/2014 entitled "Punch for Hair Transplant," filed December 15, 2014; and Indian Provisional Application No. 4161/MUM/2014 entitled "Implantation of Follicular Grafts," filed December 26,2014, PCT Application No. PCT/IN2015/050042 entitled "Hairtransplant systems and methods for their use," filed June 5, 2015, all of which are incorporated herein by reference in their entirety.
TECHNICAL FIELD
[2] The present disclosure relates, in general to hair transplantation devices and, more particularly, to a graft (e.g., hair follicle) collection device.
BACKGROUND
[3] The following description is provided to assist the understanding of the reader. None of the information provided or references cited is admitted to be prior art. In some instances, while transplanting harvested grafts (e.g., skin grafts), the recipient's body may reject the implanted grafts. In other instances, the grafts may be damaged while being harvested, transported, and/or implanted. In such instances, the graft material may not survive and may die after being transplanted.
SUMMARY
[4] An illustrative device comprises a cap and a body. The cap includes a cap inlet port and a cap outlet port in fluid communication with one another. The cap inlet port is configured to engage an inlet tube through which a graft is received. The cap outlet port is in fluid communication with a graft storage volume that is formed at least in part by the cap. The body is coupled to the cap and forms at least a portion of the graft storage volume. The body includes a body inlet port and a body outlet port in fluid communication with one another. The body outlet port is configured to engage a vacuum tube to draw the graft into the graft storage volume. The vacuum tube provides a vacuum pressure that causes a temperature of the graft storage volume to be less than an ambient temperature.
[5] An illustrative device comprises a cap and a body. The cap includes a first inlet port and a first outlet port in fluid communication with one another. The first inlet port is configured to engage an inlet tube through which a graft is received. The cap further includes a second inlet port and a second outlet port in fluid communication

with one another. The second outlet port is configured to engage a vacuum tube to draw the graft into a graft storage volume. The vacuum tube provides a vacuum pressure that causes a temperature of the graft storage volume to be less than an ambient temperature. The first outlet port is in fluid communication with the graft storage volume that is formed at least in part by the cap. The body is coupled to the cap and forms at least a portion of the graft storage volume.
[6] An illustrative method comprises inserting a volume of liquid into a graft storage module that has an inlet port and a vacuum port. The liquid is configured to moisturize a graft. The method also includes attaching a vacuum tube to the vacuum port and applying, via the vacuum tube, a suction to the graft storage module that causes a temperature of the liquid to be less than an ambient temperature. The method further comprises causing, using the suction, the graft to enter into the graft storage module via the inlet port of the graft storage module.
[7] The foregoing summary is illustrative only and is not intended to be in any way limiting. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features will become apparent by reference to the following drawings and the detailed description.
BRIEF DESCRIPTION OF THE DRAWINGS
[8] Fig. 1A is an isometric view of a graft storage module from an outside perspective in accordance with an illustrative embodiment.
[9] Fig. IB is an exploded view of the graft storage module of Fig. 1 in accordance with an illustrative embodiment.
[10] Fig. 2 is a cross-sectional view of a graft storage module in accordance with an illustrative embodiment.
[11] Fig. 3 is an internal view of a storage chamber of a graft storage module in accordance with an illustrative embodiment.
[12] Fig. 4A is an illustration of a graft storage module connected to hoses in accordance with an illustrative embodiment.
[13] Fig. 4B is a cross-sectional view of a graft storage module connected to hoses in accordance with an illustrative embodiment.
[14] Figs. 5A-5D illustrate a liquid-filled graft storage module in various orientations in accordance with an illustrative embodiment.
[15] Fig. 6 depicts a graft storage module attached to a graft extraction module via a latch in accordance with an illustrative embodiment.
[16] Figs. 7A-7C are illustrations of a single-piece latch in accordance with an illustrative embodiment.

[17] Figs. 8A-8C are illustrations of a double-piece latch in accordance with an illustrative embodiment.
[18] Figs. 9A and 9B illustrate a latch molded to the graft storage module in accordance with an illustrative embodiment.
[19] Fig. 10A is an isometric view of a vertical graft storage module from an outside perspective in accordance with an illustrative embodiment.
[20] Fig. 10B is an exploded view of the vertical graft storage module of Fig. 10A in accordance with an illustrative embodiment.
[21] Figs. 11A and 11B are cross-sectional views of a vertical graft storage module in accordance with an illustrative embodiment.
[22] Figs. 12A and 12B are isometric views of a cap of a vertical graft storage module in accordance with an illustrative embodiment.
[23] Fig. 13 depicts a spatula in accordance with an illustrative embodiment.
[24] Figs. 14A-14C are illustrations of a spatula in accordance with an illustrative embodiment.
[25] Fig. 15 is a flow diagram illustrating a method of storing harvested grafts in accordance with an illustrative embodiment.
[26] The foregoing and other features of the present disclosure will become apparent from the following description and appended claims, taken in conjunction with the accompanying drawings. Understanding that these drawings depict only several embodiments in accordance with the disclosure and are, therefore, not to be considered limiting of its scope, the disclosure will be described with additional specificity and detail through use of the accompanying drawings.
DETAILED DESCRIPTION
[27] In the following detailed description, reference is made to the accompanying drawings, which form a part hereof. In the drawings, similar symbols typically identify similar components, unless context dictates otherwise.
[28] Graft transplantation is a medical procedure performed for a variety of reasons. The graft can be any suitable graft and can include a harvested graft from a donor (who may also be the recipient of the graft) or may include an artificial graft. For example, a portion of skin can be harvested from a donor and transplanted into the recipient. In an illustrative example, a hair transplant can be performed using graft transplantation. The hair transplant can be performed by harvesting multiple hair follicles from a donor (e.g., from the back of the recipient's head) and transplanting the hair follicles to a location on the donor (e.g., on the top of the recipient's head).

[29] In illustrative embodiments, a graft in hair transplantation context is an elongated tissue surgically extracted from the donor site with the help of a punch with at least one hair within it placed almost parallel to the axis of the graft. The tissue of the graft consists of a layer of skin on top followed by dermal tissue and loose fatty tissue. In some cases it may also contain a layer of cutaneous tissue. In other embodiments, any suitable graft may be used.
[30] In many instances, care should be taken to protect and nurture the graft as soon as possible after the graft is harvested and until the graft is transplanted into the recipient. For example, it is beneficial to prevent or reduce desiccation or drying of the graft and to keep the cells of the graft in a healthy state. Reducing desiccation and maintaining healthy grafts increases the chances of a successful transplantation. Reducing desiccation also helps to prevent cell death of the grafts and to reduce microbial activity on the grafts.
[31] In some embodiments, the grafts include stem cells. The stem cells can be damaged by desiccation, lack of nutrients, or trauma (e.g., impact, pressure, temperature, etc.). Damage to the stem cells can lead to poor hair growth and less hair shaft caliber of the new hair. The damage to the stem cells is often not perceptible without analyzing the graft using machinery, tests, diagnostics, etc. For example, biological tests in a laboratory are used to determine whether stem cells are damaged. However, in most instances, it is not feasible to test the grafts for stem cell damage before implantation. Accordingly, the grafts should be protected such that as few grafts as possible are damaged. Thus, between harvesting the graft and implanting the graft, the graft should be cared for by storing the graft with the suitable amount of moisture and nutrients and at the proper temperature.
[32] Fig. 1A is an isometric view of a graft storage module from an outside perspective in accordance with an illustrative embodiment. Fig. IB is an exploded view of the graft storage module of Fig. 1 in accordance with an illustrative embodiment. The graft storage module 100 includes a cap 105, a storage chamber 110, a first port 115, a connector 120, at least one groove 125, at least one seal 130, a first hollow tube feature 135, and a cap stop 160. In alternative embodiments, additional, fewer, and/or different elements may be included. Additionally, Figs. 1A and IB are meant to be illustrative only and are not meant to be limiting with respect to the size, orientation, scale, or proportions of the illustrated elements.
[33] The graft storage module 100 can be used to store the grafts for a time after extraction of the grafts from the donor. After a sufficient amount of grafts have been collected, the graft storage module 100 can be disconnected from tubing, opened, and the contents can be poured out of the graft storage module 100. The grafts can be poured into any suitable container for transplantation into the recipient.
[34] An internal storage volume of the graft storage module 100 is defined, at least in part, by the storage chamber 110 and the cap 105. In an illustrative embodiments, the internal storage volume is used to store or house grafts (e.g., hair follicles). For

example, a graft extraction module (e.g., the graft extraction module 650 illustrated in Fig. 6) is used to harvest a graft from a donor. The harvested graft can be transported to and stored in the internal storage volume of the graft storage module 100. As discussed in greater detail below, the internal storage volume is configured to maximize the chances that the harvested grafts will be successfully implanted into the recipient.
[35] In an illustrative embodiment, the cap 105 and the storage chamber 110 are detachable. In some embodiments, the storage chamber 110 includes a cap stop 160. The cap stop 160 acts as a stop for the cap 105 as the cap 105 slides over the storage chamber 110. The cap stop 160 can also be used as an ergonomic grip or as a cosmetic detail. In some embodiments, the cap stop 160 may not be included.
[36] As shown in the embodiment illustrated in Fig. IB, the cap 105 can slide over a surface of the storage chamber 110 that has grooves 125 that receive seals 130. In alternative embodiments, the grooves 125 are located on an internal surface of the cap 105. In other embodiments, any suitable sealing mechanism can be used. Although Fig. IB illustrates the use of two grooves 125 and two seals 130, any suitable number of grooves 125 and seals 130 may be used. For example, one groove 125 and one seal 130 may be used. In another example, three or more grooves 125 and seals 130 may be used.
[37] The seals 130 can be any suitable seal. For example, the seals 130 can be 0-rings. In alternative embodiments, the seals 130 can include gaskets, clamps, etc. For example, the seals 130 can be elastomeric seals that are an over-molded design. The seals 130 can be permanently attached or removable. The seals 130 can be made of any suitable material that is bio-compatible. For example, the seals 130 can be made of Buna-N (Nitrile), ethylene-propylene, silicone, polyurethane, neoprene, one or more fluorocarbon materials, etc. In an illustrative embodiment, seals 130 are not used. In such an embodiment, an airtight interference fit between the cap 105 and the storage chamber 110 can be used to seal the cap 105 and the storage chamber 110.
[38] As shown in Fig. IB, in an illustrative embodiment, the cap 105 can be pushed onto and pulled off of the storage chamber 110. The seals 130 can provide the friction and/or resistance for securing the cap 105 to the storage chamber 110. In some instances, a vacuum pressure in the internal storage volume can be used to assist in securing the cap 105 to the storage chamber 110. In alternative embodiments, any other suitable arrangement for securing the cap 105 and the storage chamber 110 to one another may be used. For example, the cap 105 and the storage chamber 110 can comprise threads that allow the cap 105 to screw onto the storage chamber 110. In another example, clips or clamps may be used. For example, the cap 105 and the storage chamber 110 may be secured together using a snap joint, a Luer Lock, a magnetic interference, etc.

[39] Fig. 2 is a cross-sectional view of an illustrated graft storage module in accordance with an illustrative embodiment. The graft storage module 100 includes a cap 105, a storage chamber 110, a first port 115, a connector 120, at least one seal 130, a first hollow tube feature 135, a second hollow tube feature 140, and a retaining filter 145. In alternative embodiments, additional, fewer, and/or different elements may be included in the graft storage module.
[40] In an illustrative embodiment, the connector 120 is connected to a vacuum source. For example, a vacuum tube (e.g., flexible vacuum hose 155 illustrated in Fig. 4A) can be attached to the connector 120. Fig. 2 illustrates the connector 120 with a barbed fitting. In alternative embodiments, any suitable connection can be used to connect the vacuum tube to the graft storage module 100. For example, a threaded fitting, a snap connection, a quick disconnect fitting, etc. may be used.
[41] The first port 115 can be attached to a graft source. For example, a graft collection tube 150 (as shown in Fig. 4A) is connected to the first port 115 and provides a non-obstructed path for the grafts to travel. In another example, the first port 115 is directly connected to a graft extraction module (e.g., without the use of the graft collection tube 150). A harvested graft can be suctioned into the internal storage volume of the graft storage module 100 using the vacuum provided through the connector 120. That is, a vacuum source can be connected to the connector 120, and the vacuum pressure can be used to pull the harvested graft into the first port 115 and through the first hollow tube feature 135, which are fluidly connected.
[42] The harvested graft is pulled through the first hollow tube feature 135 and into the internal storage volume. The internal storage volume can store multiple grafts. As discussed in greater detail below, the internal storage volume can also include a liquid, such as saline. Thus, in an illustrative embodiment, after the graft is pulled through the hollow tube feature 135, the graft can fall into the liquid in the internal storage volume, in which the graft can remain in storage, for example, while additional grafts are harvested. In some embodiments, the storage chamber 110 is marked with graduated markings that can be used to indicate the volume of liquid (and grafts) in the internal storage volume.
[43] As illustrated in Fig. 2, in some embodiments, the first hollow tube feature 135 extends into the internal storage volume. The first hollow tube feature 135 can be centered on the cap 105 and extend into the internal storage volume parallel to the sides of the storage chamber 110. In embodiments in which the sides of the storage chamber 100 are not parallel, the first hollow tube feature 135 can extend along a center axis of the graft storage module that passes through the first port 115 and the connector 120. In alternative embodiments, the first hollow tube feature 135 can be flush with (e.g., not extend beyond) the inner surface of the cap 105. In such embodiments, vacuum pressure from the connector 120 can cause air to flow into the internal storage volume (and out of the internal storage volume via the second hollow tube feature 140 and the connector 120). The air flowing through the first hollow tube feature 135 can prevent the pre-determined amount of liquid within the internal

storage volume from entering the first hollow tube feature 135 (and, subsequently, the first port 115, a graft collection tube 150, and/or a graft extraction module).
[44] In alternative embodiments, a one-way valve can be placed within the first hollow tube feature 135 that allows grafts to enter the internal storage volume, but prevents the liquid from entering the graft collection tube 150. In such embodiments, the one-way valve can be implemented within the connection between the graft collection tube 150 and the first port 115. The use of a one-way valve retains a negative pressure between the one-way valve and the vacuum source, thereby improving the response time of the system.
[45] In some embodiments, the vacuum applied to the connector 120 is applied continuously. That is, in such embodiments, vacuum is applied to the connector 120 while grafts are stored in the internal storage volume and/or while grafts are being harvested. In alternative embodiments, the vacuum is applied to transport the grafts into the internal storage volume and is shut off in between harvesting of the individual grafts. Switching on and off of the vacuum can be manual or automatic using any suitable method.
[46] As shown in Fig. 2, the connector 120 is fluidly connected to the second hollow tube feature 140. The second hollow tube feature 140 extends into the internal storage volume. The second hollow tube feature 140 can be centered on the storage chamber 110 and extend into the internal storage volume parallel to the sides of the storage chamber 110. In embodiments in which the sides of the storage chamber 100 are not parallel, the second hollow tube feature 140 can extend along the center axis of the graft storage module that passes through the first port 115 and the connector 120.
[47] In the embodiment illustrated in Fig. 2, the first hollow tube feature 135 and the second hollow tube feature 140 extend into the internal storage volume substantially along the same central axis of the graft storage module 100. In alternative embodiments, the first hollow tube feature 135 and the second hollow tube feature 140 do not extend along a central axis and/or are not parallel to the sides of the storage chamber 110. In such embodiments, the opening of the first hollow tube feature 135 and the retaining filter 145 are located along the central axis even though the first hollow tube feature 135 and the second hollow tube feature 140 do not connect to the cap 105 and the storage chamber 110 along the central axis.
[48] |n the embodiment illustrated in Fig. 2, the first hollow tube feature 135 and the second hollow tube feature 140 extend into the internal storage volume substantially along the same central axis of the graft storage module 100. The first hollow tube feature 135 and the second hollow tube feature 140, however, do not touch one another. Rather, the first hollow tube feature 135 and the second hollow tube feature 140 are spaced apart. In an illustrative embodiment, the first hollow tube feature 135 and the second hollow tube feature 140 are spaced apart enough to allow a graft to flow through the first hollow tube feature 135 and into liquid within the

internal storage volume. In an illustrative embodiment, the first hollow tube feature 135 and the second hollow tube feature 140 are spaced apart by 10 millimeters (mm). In alternative embodiments, the first hollow tube feature 135 and the second hollow tube feature 140 are spaced apart by less than 10 mm or greater than 10 mm. For example, the first hollow tube feature 135 and the second hollow tube feature 140 can be spaced apart by 6 mm, 8 mm, 9 mm, 9.5 mm, 10.5 mm, 11 mm, 12 mm, 14 mm, etc. In some instances, the separation between the first hollow tube feature 135 and the second hollow tube feature 140 is chosen to be greater than the length of the longest graft that is intended to be harvested.
[49] Fig. 3 is an internal view of a storage chamber of a graft storage module in accordance with an illustrative embodiment. In alternative embodiments, additional, fewer, and/or different elements may be used. As shown in Figs. 2 and 3, the second hollow tube feature 140 can include a retaining filter 145. The retaining filter 145 can be configured to prevent grafts from entering into the second hollow tube feature 140. In the embodiments illustrated in Figs. 2 and 3, the retaining filter 145 includes slits along the second hollow tube feature 140. The slits can be shaped and sized such that the grafts cannot fit within the slits. The retaining filter 145 separates and retains the grafts inside the internal storage volume. In some instances, the retaining filter 145 allows excess fluid (e.g., air, liquid, saline, etc.) to drain through the flexible vacuum hose 155, for example, to an external collection bottle.
[50] In some embodiments, the grafts are harvested using a graft extraction module that punches grafts out of the donor's tissue. The length of the grafts can be determined by the thickness of the skin from which the grafts are harvested. In an illustrative embodiment, the length of the grafts can be between 6 mm and 8 mm. For example, the grafts can be 6 mm long, 6.5 mm long, 7 mm long, 7.5 mm long, 8 mm long, etc. In alternative embodiments, the grafts can be shorter than 6 mm or longer than 8 mm. In embodiments in which the graft includes a hair follicle, hair can protrude from the graft. The length of the hair can be as long as a hair can grow. In some instances, the hair is shaven close to the skin. In other embodiments, the hair is trimmed prior to extraction to be approximately 2 mm to 5 mm above the surface of the skin.
[51] The diameter of the grafts depends on the size of the punch or cutting member of the graft extraction module (or other method/tool used to harvest the graft). In illustrative embodiments, the grafts can be from 0.8 mm in diameter to 1.2 mm in diameter. For example, the diameter of the grafts can be 0.8 mm, 0.9 mm, 1.0 mm, 1.1 mm, 1.2 mm, etc. In alternative embodiments, the grafts can be less than 0.8 mm in diameter or greater than 1.2 mm in diameter. In an illustrative embodiment, the thickness of the slits of the retaining filter 145 is one half of the diameter of the grafts. Thus, in illustrative embodiments, the thickness of the slits can be from 0.4 mm thick to 0.6 mm thick. For example, the slits can be 0.4 mm thick, 0.5 mm thick, 0.55 mm thick, 0.6 mm thick, etc. In alternative embodiments, the slits can be less than 0.4 mm thick or greater than 0.6 mm thick.

[52] In alternative embodiments, the retaining filter 145 can include a filtering element other than slits. For example, a mesh filter or a screen can be used. In some instances, a retaining filter 145 can be selected to minimize the air flow resistance through the retaining filter 145. In the embodiments illustrated in Figs. 2 and 3, the retaining filter 145 is permanently fixed to the second hollow tube feature 140. In alternative embodiments, the retaining filter 145 can be removable. For example, the retaining filter 145 can be removably attached to the second hollow tube feature 140 via threads, a snap connection, a quick disconnect, etc.
[53] Fig. 4A is an illustration of a graft storage module connected to hoses in accordance with an illustrative embodiment. Fig. 4B is a cross-sectional view of a graft storage module connected to hoses in accordance with an illustrative embodiment. In alternative embodiments, additional, fewer, and/or different elements may be used. In an illustrative embodiment, a graft collection tube 150 can be attached to the cap 105 via the first port 115. As illustrated in Fig. 4B, the graft collection tube 150 can be received by the first port 115 and the first port 115 can surround a portion of the graft collection tube 150. The outer diameter of the graft collection tube 150 and the inner diameter of the first port 115 can be sized such that there is sufficient friction between the graft collection tube 150 and the first port 115 to secure the graft collection tube 150 to the first port 115.
[54] In the embodiment illustrated in Fig. 4B, a push-style connection is used. That is, the graft collection tube 150 is pushed into the first port 115 to connect the graft collection tube 150 and the first port 115. In alternative embodiments, any suitable connection style may be used. For example, the connection between the graft collection tube 150 and the first port 115 can include threads, a snap, a compression fitting, etc. In some embodiments, the connection between the graft collection tube 150 and the first port 115 can be glued, fused, over molded, etc. In alternative embodiments, the graft collection tube 150 is molded to the cap 105 as a single unit.
[55] The inside diameter of the graft collection tube 150 can be selected to be the same size or slightly larger than the diameter of the harvested grafts. The inside diameter of the graft collection tube 150 can be selected to be large enough that the harvested grafts can travel through the graft collection tube 150, but not so large that the vacuum applied to suction the grafts through the graft collection tube 150 is not strong enough to cause the graft to be pulled through the graft collection tube 150.
[56] For example, the inside diameter of the graft collection tube 150 can be up to 10% wider than the width of the harvested grafts. In an illustrative embodiment, the graft collection tube 150 can be 1%, 2%, 5%, 7%, or 10% wider than the harvested grafts. In alternative embodiments, the graft collection tube 150 can be greater than 10% wider than the harvested grafts. In some embodiments, the graft collection tube 150 is around 50% wider than the harvested grafts. For example, the graft collection tube 150 is 35%, 40%, 45%, 50%, 55%, 60%, 65%, etc. wider than the harvested grafts. For example, a rotary punch can be used to harvest grafts with a diameter of 1 mm. Thus, in such an example, the graft collection tube 150 can have an inside diameter of

1 mm. In another example, the graft collection tube 150 can have an insjde diameter of 1.1 mm or 1.05 mm.
[57] The length of the graft collection tube 150 can be any suitable length. In some embodiments, the length of the graft collection tube 150 is chosen to be as short as possible or convenient. In some instances, as the graft travels through the graft collection tube 150 via vacuum pressure, air flows between the inside wall of the graft collection tube 150 and the graft. The airflow can dry out the graft. Additionally, the longer that the graft collection tube 150 is, the further the graft travels and slides against the graft collection tube 150. Friction between the graft collection tube 150 and the graft can damage the graft. Accordingly, in some embodiments, shortening the length of the graft collection tube 150 can reduce the amount of desiccation of the grafts. In alternative embodiments, the first port 115 can be directly connected to a graft extraction module, thereby eliminating the graft collection tube 150.
[58] As shown in Fig. 4B, the inside diameter of the first hollow tube feature 135 is the same as the inside diameter of the graft collection tube 150. In alternative embodiments, the relative inside diameters of the first hollow tube feature 135 and the graft collection tube 150 can be chosen such that the harvested grafts can be suctioned through the graft collection tube 150 and into the first hollow tube feature 135 without damaging the harvested graft. For example, in some embodiments, the inside diameter of the first hollow tube feature 135 is larger than the inside diameter of the graft collection tube 150. In some embodiments, the inside surfaces of the graft collection tube 150 and/or the first hollow tube feature 135 can include a lubrication material. For example, the inside surfaces of the graft collection tube 150 and/or the first hollow tube feature 135 can be coated with a hydrophobic or hydrophilic coating. Such a coating can reduce sticking of the graft and/or other biologic material to the surfaces of the graft collection tube 150 and/or the first hollow tube feature 135. The coating can also reduce the friction between the graft and the graft collection tube 150 and/or the first hollow tube feature 135, thereby reducing damage of the graft. The coating can further prevent (or reduce) clotting of the blood of the graft, thereby increasing the chance that the graft will be successfully transplanted. In some embodiments, the surfaces of the first hollow tube feature 135 and/or the second hollow tube feature 140 are textured with an anti-sticking property. For example, nano-texturing may be used.
[59] Also as shown in Fig. 4B, in some embodiments, the inside diameter of the second hollow tube feature 140 can be the same as the inside diameter of the first hollow tube feature 140 and/or the graft collection tube 150. In alternative embodiments, the inside diameter of the second hollow tube feature 140 can be the same as the inside diameter of the flexible vacuum hose 155. Although Fig. 4B illustrates the flexible vacuum hose 155 connected to the storage chamber 110 via a barbed fitting, in alternative embodiments, any suitable connection can be used to connect the vacuum tube to the graft storage module 100. For example, a threaded fitting, a snap connection, a quick disconnect, etc. may be used.

[60] The length that the second hollow tube feature 140 protrudes into the internal storage volume of the graft storage module 100 can be chosen such that liquid in the internal storage volume is not suctioned into the second hollow tube feature 140. The length of the first hollow tube feature 135 can be similarly chosen. Figs. 5A-5D illustrate a liquid-filled graft storage module in various orientations in accordance with an illustrative embodiment. Figs. 5A-5D illustrate the liquid level in the graft storage module 100 in positions ranging from vertical (Fig. 5A) through horizontal (Fig. 5D). As illustrated in Figs. 5A-5D, the liquid level can be chosen such that, regardless of orientation, the liquid level 170 does not reach the opening of the second hollow tube feature 140. However, there is still enough liquid to cover and moisturize the harvested grafts 165. In some instances, saline (or other liquid) can be suctioned through the graft collection tube 150 and into the internal storage volume. In such instances, excess liquid within the internal storage volume is suctioned through the second hollow tube feature.
. [61] For example, the second hollow tube feature 140 can protrude into the internal storage volume by a length of between 30 mm and 60 mm. For example, the second hollow tube feature 140 can protrude into the internal storage volume by 30 mm, 35 mm, 40 mm, 45, mm, 50 mm, 55 mm, 60 mm, etc. In alternative embodiments, the second hollow tube feature 140 can protrude into the internal storage volume by less than 30 mm or by more than 60 mm. In some embodiments, the length of the second hollow tube feature 140 includes the length of the retraining filter 145. In alternative embodiments, the length of the second hollow tube feature 140 does not include the length of the retaining filter 145.
[62] Similarly, the first hollow tube feature 135 can protrude into the internal storage volume by a length of between 30 mm and 60 mm. For example, the first hollow tube feature 135 can protrude into the internal storage volume by 30 mm, 35 mm, 40 mm, 45, mm, 50 mm, 55 mm, 60 mm, etc. In alternative embodiments, the first hollow tube feature 135 can protrude into the internal storage volume by less than 30 mm or by more than 60 mm.
[63] The liquid used within the internal storage volume can be any suitable liquid to preserve the grafts in a healthy state. For example, the liquid can be water. In some instances, the liquid can be a saline solution. In other instances, the liquid can be a saline solution with added compounds. The additional compounds can include nutrients for the grafts, moisturizers, etc. As grafts are harvested and collected in the internal storage volume, the liquid may include biological matter (e.g., skin, blood, etc.) that is incident to the harvesting of the grafts.
[64] The cap 105 and the storage chamber 110 can be made of any suitable biocompatible materials. In some embodiments, at least a portion of at least one of the cap 105 or the storage chamber 110 is transparent or translucent to allow a clinician to view the liquid and/or the grafts collected in the internal storage volume. For example, the cap 105 and/or the storage chamber 110 can be made of polycarbonate, glass, stainless steel, etc. Although the shape of the cap 105 and the storage chamber

110 are illustrated as being cylindrical, any suitable shape can be used. For example, the cross-sectional shape of the cap 105 and the storage chamber 110 can be circular, elliptical, octagonal, rectangular, square, or any other geometric or non-geometric shape.
[65] The inside diameter and the length of the storage chamber 110 (and/or the cap 105) can be selected to allow enough liquid to be stored in the internal storage volume to preserve the health of the grafts without the level of the liquid reaching the opening of the second hollow tube feature 140. For example, the inside diameter of the storage chamber 110 (and/or the cap 105) can be 25 mm. In alternative embodiments, the inside diameter of the storage chamber 110 can be less than or greater than 25 mm. For example, the inside diameter of the storage chamber 110 can be 10 mm, 15 mm, 20 mm, 30 mm, 35 mm, 40 mm, etc. In an illustrative embodiment the inside length of the internal storage volume is 100 mm. In alternative embodiments, the inside length of the internal storage volume can be less than or greater than 100 mm. For example, the inside length of the internal storage volume can be 80 mm, 90 mm, 95 mm, 105 mm, 110 mm, 120 mm, etc.
[66] The graft collection tube 150 may be made of any suitable bio-compatible material. In some embodiments, the graft collection tube 150 can be transparent or translucent to allow a clinician to view the graft (or other material) in the graft collection tube 150. In some embodiments, the graft collection tube 150 is rigid. In such embodiments, the graft collection tube 150 can be made of, for example, polycarbonate or stainless steel. In other embodiments, the graft collection tube 150 is flexible or semi-flexible. For example, the graft collection tube 150 can be made of a thermoplastic elastomer, polyvinyl chloride, polyether ether ketone (PEEK), polyurethane, silicone, nylon, poly ethylene, poly phenyl sulphone, glass, ceramic, composite materials such as glass reinforced plastic or carbon composites, etc. Because grafts are suctioned through the graft collection tube 150 using vacuum, the graft collection tube 150 is rigid enough not to collapse under the vacuum pressure.
[67] Fig. 6 depicts a graft storage module attached to a graft extraction module via a latch in accordance with an illustrative embodiment. In alternative embodiments, additional, fewer, and/or different elements may be used. As discussed above, some embodiments of the graft storage module 100 can be oriented in any direction while maintaining a pre-determined amount of liquid in the internal storage volume. Thus, in some embodiments, the graft storage module 100 can be attached to a graft extraction module 650. The graft extraction module 650 can be any suitable device, and the graft extraction module 650 illustrated in Fig. 6 is illustrative only. In alternative embodiments, the graft storage module 100 can be attached to any suitable device.
[68] The graft storage module 100 can be attached to the graft extraction module 650 via a latch 600. In some embodiments, the latch 600 can be a clip. Figs. 7A-7C are illustrations of a single-piece latch in accordance with an illustrative embodiment. Figs. 8A-8C are illustrations of a double-piece latch in accordance with an illustrative

embodiment. In alternative embodiments, additional, fewer, and/or different elements may be used. The latch 600 can be used to prevent misplacing the graft storage module 100 or prevent the graft storage module 100 from freely moving around and becoming a distraction to clinicians. Additionally, the clip-like latch 600 allows the graft storage module 100 to be located along a convenient location of the graft extraction module 650. That is, in some instances, the latch 600 can secure the graft storage module 100 at a plurality of locations along the graft extraction module 650.
[69] Fig. 7A is an isometric view of a single-piece latch 600. Fig. 7B is a top view of the single-piece latch 600. Fig 7C is a side view of the single-piece latch 600. The single-piece latch 600 can be made of a single piece of material, such as plastic, metal, or a composite. For example, the single-piece latch 600 can be molded from polycarbonate or can be machined from billet aluminum or stainless steel.
[70] Fig. 8A is an isometric view of a double-piece latch 600. Fig. 8B is a top view of the double-piece latch 600. Fig 8C is a side view of the double-piece latch 600. The double-piece latch 600 can be formed by two pieces joined in the middle. The two pieces can be joined using any suitable method, such as by welding, with adhesives, with rivets, with screws, etc. The two pieces can be made of any suitable material, such as plastic, metal, or a composite. For example, the pieces can be made of polycarbonate, aluminum, stainless steel, etc.
[71] One C-shaped end of the latch 600 can be configured to be secured around at least a portion of the graft storage module 100. The other C-shaped end of the latch 600 can be configured to be secured around at least a portion of the graft extraction module 650 or any other suitable device or fixation point. The C-shaped ends can clip onto the respective device. The C-shaped ends can be manufactured such that the C-shaped ends provide an optimum stiffness to its jaws for secure holding of the graft extraction module 650, the graft storage module 100, etc. In an illustrative embodiment, both of the C-shaped ends are identical. In alternative embodiments, the C-shaped ends can be differently shaped from one another (e.g., be different sizes). For example, the size and shape of one of the C-shaped ends corresponds to the size and shape of the graft storage module 100, and the size and shape of the other C-shaped end corresponds to the size and shape of the graft extraction module 650.
[72] In some embodiments, one end of the latch 600 can be permanently secured and/or molded into either the graft storage module 100 or the graft extraction module 650 (or any other suitable device). In some embodiments, one end of the latch 600 is permanently secured and/or molded to the graft storage module 100 and the other end of the latch 600 is permanently secured and/or molded to the graft extraction module 650 (or any other suitable device).
[73] Figs. 9A and 9B illustrate a latch molded to the graft storage module in accordance with an illustrative embodiment. In alternative embodiments, additional,

fewer, and/or different elements may be used. Fig. 9A is an isometric view of the graft storage module 100 with a molded latch 600, and Fig. 9B is a cross-sectional side view of the graft storage module 100 with a molded latch 600. A graft storage module 100 with the molded latch 600 can be economical to manufacture and can prevent misplacing the latch 600.
[74] As discussed above, a vacuum source can be attached to the connector 120, which can cause a vacuum pressure to be in the internal storage volume and the graft collection tube 150. Any suitable amount of vacuum can be applied at the connector 120. For example, the pressure at the connector 120 can be in the range of 200 millimeters of mercury (mm Hg) to 700 mm Hg. For example, the pressure at the connector 120 can be 200 mm Hg, 300 mm Hg, 400 mm Hg, 450 mm Hg, 500 mm Hg, 550 mm Hg, 600 mm Hg, 700 mm Hg, etc. In alternative embodiments, the pressure at the connector 120 can be less than 200 mm Hg or greater than 700 mm Hg. The amount of vacuum pressure used can be chosen by a clinician based on the size of the graft storage module 100 (and/or its various components), the size of the various tubes, the size of the grafts, the size and type of the graft extraction module used, the particular conditions of the patient, the particular atmospheric conditions, the preference of a clinician, etc. For example, the vacuum pressure used can be chosen such that the amount of suction applied at the harvesting location overcomes tissue adherence of the graft below the follicular bulb.
[75] The internal storage volume can include a liquid under vacuum pressure. As air is vacuumed out of the internal storage volume, the temperature of the internal storage volume (and, therefore, the temperature of the liquid and the grafts) can be decreased by 8° C to 10° C. For example, the temperature of the internal storage volume can be 8° C, 8.5° C, 9° C, 9.5° C, 10° C, etc. lower than the ambient temperature (e.g., about 23° C). In alternative embodiments, the temperature of the internal storage volume can be less than 8°C lower than the ambient temperature or greater than 10° C lower than the ambient temperature. In some embodiments, a temperature indicator is attached to the graft storage module 100 that indicates the temperature of the internal storage volume. Any suitable temperature indicator can be used.
[76] In some embodiments, a cooling system is incorporated into the graft storage module 100 to maintain the temperature of the internal storage volume at a setpoint, which may be chosen by the clinician. Any suitable cooling system may be used. For example, the cooling system can provide cool air to the internal storage chamber, thereby cooling the liquid. In another example, the cooling system can provide cool saline to the internal storage chamber, thereby displacing some of the liquid already in the internal storage chamber. In other embodiments, a cooling jacket can surround (at least a portion of) the graft storage system 100. A cooling fluid (e.g., gas or liquid) can circulate around the outside surface of the graft storage system 100, thereby transferring heat out of the internal storage volume. In some embodiments, a cooling fluid passes through the internal storage volume. For example, a closed-loop system can circulate cooling fluid through the internal storage volume via one or more tubes

(e.g., tubes perpendicular or parallel to the first hollow tube feature 135 and the second hollow tube feature 145).
[77] Storing the grafts in the range of 8° C to 10° C less than ambient room temperature increases the chances of a successful transplantation of the grafts compared to storing the grafts at ambient temperature or at colder temperatures, such as at 4° C. At ambient temperatures, microbial activity can damage cells of the grafts. Thus, by cooling the grafts, microbial activity can be suspended, thereby ceasing damage to the grafts caused by the microbial activity. Cooling the grafts suspends tissue metabolism, thereby preserving the grafts. However, cooling the grafts down to about 4° C (e.g., sudden cooling to 4° C) can also cause damage to or death of the cells of the grafts and can lead to an increased transplantation failure rate. Thus, grafts are harvested and have a temperature of about 37° C (body temperature) and are stored in the liquid in the internal storage volume. The grafts are then cooled to about 8° C to 10° C below ambient temperature until they are implanted in the recipient, when the grafts warm back up to about 37° C.
[78] In alternative embodiments, a vertical graft storage module 1000 can be used to collect harvested grafts in place of the graft storage module 100. Fig. 10A is an isometric view of a vertical graft storage module from an outside perspective in accordance with an illustrative embodiment. Fig. 10B is an exploded view of the vertical graft storage module of Fig. 10A in accordance with an illustrative embodiment. Figs. 11A and 11B are cross-sectional views of a vertical graft storage module in accordance with an illustrative embodiment. Figs. 12A and 12B are isometric views of a cap of a vertical graft storage module in accordance with an illustrative embodiment. An illustrative graft storage module 1000 includes a cap 1005, a storage chamber 1010, a first port 1015, a connector 1020, at least one groove 1025, at least one seal 1030, a first hollow tube feature 1035, a retaining filter 1045, a graft collection tube 1050, and a spatula 1075. In alternative embodiments, additional, fewer, and/or different elements may be used. Additionally, Figs. 10A and 10B are meant to be illustrative only and are not meant to be limiting with respect to the size, orientation, scale, or proportions of the illustrated elements.
[79] The internal storage volume of the graft storage module 1000 is defined, at least in part, by the storage chamber 1010 and the cap 1005. The size and materials of construction of the cap 1005 and the storage chamber 1010 can be the same as those discussed above with regard to the cap 105 and the storage chamber 110 of the graft storage module 100. In an illustrative embodiment, the cap 1005 and the storage chamber 1010 are detachable. As shown in the embodiment illustrated in Fig. 10B, the cap 1005 can slide over a surface of the storage chamber 1010 that has grooves 1025 that receive seals 1030. The grooves 1025 and seals 1030 can be similar in form and function as grooves 125 and seals 130 discussed above in regard to graft storage module 100. In alternative embodiments, the grooves 1025 are located on an internal surface of the cap 1005. In other embodiments, any suitable sealing mechanism can be used. Although Fig. 10B illustrates the use of two grooves 1025 and two seals 1030, any suitable number of grooves 1025 and seals 1030 may be used. For example, one

groove 1025 and one seal 1030 may be used. In another example, three or more grooves 1025 and seals 1030 may be used.
[80] The seals 1030 can be any suitable seal. For example, the seals 1030 can be O-rings. In alternative embodiments, the seals 1030 can include gaskets, clamps, etc. The seals 1030 can be made of any suitable material that is bio-compatible. For example, the seals 1030 can be made of Buna-N (Nitrile), ethylene-propylene, silicone, polyurethane, neoprene, one or more fluorocarbon materials, etc.
[81] As shown in Fig. 10B, in an illustrative embodiment, the cap 1005 can be pushed onto and pulled off of the storage chamber 1010. The seals 1030 can provide the friction and/or resistance for securing the cap 1005 to the storage chamber 1010. In some instances, a vacuum pressure in the internal storage volume can be used to assist in securing the cap 1005 to the storage chamber 1010. In alternative embodiments, any other suitable arrangement for securing the cap 1005 and the storage chamber 1010 to one another may be used. For example, the cap 1005 and the storage chamber 1010 can include threads that allow the cap 1005 to screw onto the storage chamber 1010. In another example, clips or clamps may be used.
[82] In an illustrative embodiment, the connector 1020 is connected to a vacuum source, such as the vacuum source discussed above with respect to the graft storage module 100. Figs. 10A, 10B, 11A, and 11B illustrate the connector 1020 with a barbed fitting. In alternative embodiments, any suitable means for connecting the vacuum tube to the graft storage module 1000 may be used. For example, a threaded fitting, a snap connection, a quick disconnect fitting, etc. may be used. The connector 1020 can include a retaining filter 1045. In some embodiments, the retaining filter 1045 includes a plurality of filtering elements. The plurality of filtering elements can be the same or different from one another. The retaining filter 1045 can be configured to prevent grafts from entering into the connector 1020. The retaining filter 1045 can be any suitable filter for preventing grafts from passing through the retaining filter 1045. The retaining filter 1045 can have any of the properties discussed above with regard to retaining filter 145. As illustrated in Figs. 11A and 11B, the retaining filter 1045 can be flush with the inside surface of the cap 1005. In alternative embodiments, the retaining filter 1045 can be recessed into the cap 1005 or protrude from the cap 1005. The retaining filter 1045 can include perforations, a screen, a mesh, etc. In an illustrative embodiment, the retaining filter 1045 is a micro filter. In some embodiments, the retaining filter 1045 can be removable/replaceable. In alternative embodiments, the retaining filter 1045 is integrated into the cap 1005.
[83] In some embodiments, the retaining filter 1045 does not prevent liquid from entering into the connector 1020, which may be under a vacuum pressure. Accordingly, if the graft storage module 1000 is tipped such that the liquid within the internal storage volume contacts the retaining filter 1045, the liquid may be suctioned into the connector 1020. Thus, to prevent suctioning the liquid into the connector 1020, the graft storage module 1000 may be kept in a substantially upright and vertical orientation to prevent the liquid from contacting the retaining filter 1045.

[84] The first port 1015 can be attached to a graft source. For example, a graft collection tube 1050 is connected to the first port 1015. A harvested graft can be suctioned into the internal storage volume of the graft storage module 1000 using the vacuum provided through the connector 1020. That is, a vacuum source can be connected to the connector 1020, and the vacuum pressure can be used to pull the harvested graft into the first port 1015 and through the first hollow tube feature 1035. In some embodiments, the first hollow tube feature 1035 is part of the graft collection tube 1050. That is, in some embodiments, the graft collection tube 1050 is inserted into the first port 1015 and into the internal storage volume such that the graft collection tube 1050 protrudes into the internal storage volume. In alternative embodiments, the graft collection tube 1050 is inserted into the first port 1015 and does not protrude into the internal storage volume. In such an embodiment, the first hollow tube 1035 can be attached to the cap 1005.
[85] The internal storage volume of the graft storage module 1000 can also include a liquid, such as saline. Thus, in an illustrative embodiment, after the graft is pulled through the hollow tube feature 1035, the graft can fall into the liquid in the internal storage volume, in which the graft can remain in storage, for example, while additional grafts are harvested. The first hollow tube 1035 protrudes below a surface of the liquid. In such embodiments, damage to the graft can be minimized by having the first hollow tube feature 1035 protrude below the surface of the liquid because, as the graft enters into the liquid, the graft can be surrounded (at least in part) in a bubble. That is, the vacuum pressure suctions air and the graft out of the first hollow tube feature 1035 simultaneously. Subsequently, the bubble can dissipate or float away from the graft, thereby gently entering the graft into the liquid. In alternative embodiments, the first hollow tube feature 1035 does not protrude below a surface of the liquid and the graft can fall into the liquid. The size of the first hollow tube feature 1035, the first port 1015, and the graft collection tube 1050 can be chosen in the same fashion as the first hollow tube feature 135, the first port 115, and the graft collection tube 150 as discussed above in regard to the graft storage module 100.
[86] In some embodiments, the vacuum applied to the connector 1020 is applied continuously. That is, in such embodiments, vacuum is applied to the connector 1020 while grafts are stored in the internal storage volume and/or while grafts are being harvested. In alternative embodiments, the vacuum is applied to transport the grafts into the internal storage volume and is shut off in between harvesting of the individual grafts. Switching on and off of the vacuum can be manual or automatic using any suitable method.
[87] Fig. 13 depicts a spatula in accordance with an illustrative embodiment. An illustrative spatula includes a perforated discus 1305 and a handle grip 1310. In alternative embodiments, additional, fewer, and/or different elements may be used. The spatula 1075 can be used to strain the harvested grafts collected in the internal storage volume from the liquid used to protect the grafts. The spatula 1075 can be located within the internal storage volume, with the perforated discus 1305 resting on the top surface of the bottom of the storage chamber 1010. In alternative

embodiments, the perforated discus 1305 can be suspended above the bottom of the storage chamber 1010.
[88] As grafts enter the internal storage volume, the grafts can enter the liquid above the perforated discus 1305. A clinician (or any suitable device) can grasp or otherwise secure the handle grip 1310, which extends vertically from the perforated discus 1305, and lift the spatula 1075 up and off of the bottom of the internal storage volume. The perforations in the perforated discus 1305 can allow the liquid to flow through the perforated discus 1305, but prevent the grafts from flowing through the perforated discus 1305. For example, the perforations can be sized similar to the retaining filter 145, which is discussed above with regard to the graft storage module 100. Thus, as the spatula 1075 is lifted, the grafts are strained from the liquid and rest on the top surface of the perforated discus 1305. The perforated discus 1305 can be sized such that the graft cannot fall between a sidewall of the storage chamber 1010 and the perforated discus 1305. The spatula 1075 can be made of any suitable biocompatible material such as rubber, plastic, metal, stainless steel, aluminum, polycarbonate, etc.
[89] A spatula (e.g., spatula 1475) similar to the spatula 1075 can be adapted for use in the graft storage module 100. Figs. 14A-14C are illustrations of a spatula in accordance with an illustrative embodiment. Fig. 14A illustrates a spatula 1475 with a perforated discus 1405, a handle grip 1410, and a through holel415. Fig. 14B is an exploded view of the spatula 1475 assembled with the graft storage module 100. Fig. 14C is a cross-sectional view of the spatula 1475 in the graft storage module 100. In alternative embodiments, additional, fewer, and/or different elements may be used.
[90] The spatula 1475 has a through hole 1415 in the perforated discus 1405. The perforations in the perforated discus 1405 can be the same as the perforations in the perforated discus 1305. The through hole 1415 allows the second hollow tube feature 140 to pass through the perforated discus 1305. Thus, the perforated discus 1305 can rest on the bottom of the storage chamber 110. The diameter of the through hole can be the same as (or slightly larger than) the outside diameter of the second hollow tube feature 140. The spatula 1475 can strain harvested grafts from the liquid when the spatula 1475 is raised out of the storage chamber 110.
[91] Fig. 15 is a flow diagram illustrating a method of storing harvested grafts in accordance with an illustrative embodiment. In alternative embodiments, additional, fewer, and/or different elements may be used. Additionally, the use of arrows and/or a flow diagram is not meant to be limiting with respect to the order or flow of operations.
[92] In an operation 1505, liquid is inserted into a graft storage module, such as the graft storage module 100 or the graft storage module 1000. The liquid can be inserted into the graft storage module in any suitable manner. For example, the liquid can be suctioned through a graft collection tube and into an internal storage volume of the graft storage module (e.g., through the cap of the graft storage module). In other

examples, the liquid can be inserted into the graft storage module by pouring the liquid, injecting the liquid from a syringe, etc. In an illustrative embodiment, the liquid is saline. The amount of liquid can be pre-determined (e.g., measured) and be dependent on the size of the graft storage module.
[93] In an operation 1510, the graft storage module is assembled. In an illustrative embodiment, assembling the graft storage module includes fixing (e.g., by sliding) the cap onto the storage chamber. In an embodiment using the graft storage module 1000, assembling the graft storage module includes placing the spatula into the storage chamber. In some embodiments, assembling the graft storage module includes fixing a filter to the graft storage module, fixing one or more seals (e.g., 0-rings) to the graft storage module, etc.
[94] In an operation 1515, an inlet hose is attached to the graft storage module. For example, the graft collection tube, as described above, is connected to the first port of the graft storage module. In an alternative embodiment, operation 1515 can comprise attaching the graft storage module (e.g., via the first port) directly to a graft extraction module. In some embodiments, the method illustrated in Fig. 15 includes mounting the graft storage module. A clip or latch can be used to secure the graft storage module to another device or mounting point. For example, the graft storage module is attached to a graft extraction device using a latch.
[95] In an operation 1520, a vacuum hose is attached to the graft storage module. The vacuum hose can be attached to the connector of a graft storage module. In an operation 1525, suction is applied to the graft storage module. For example, vacuum pressure is applied via the vacuum hose by operating a valve, operating a panel or other user interface of a vacuum control system, etc.
[96] In an operation 1530, a graft is harvested from a donor. The harvested graft can include one or more hair follicles. Harvesting the graft can be performed using any suitable method. For example, a graft extraction module is used to core out a graft. In an operation 1535, the harvested graft is stored in the graft storage module. Suction from the vacuum hose is used to cause the harvested graft to travel through a collection tube (if one is used) and enter the storage chamber.
[97] In an operation 1540, the cap of the graft storage module is removed. In an illustrative embodiment, operation 1540 is performed after several grafts have been harvested and stored in the graft storage module. In some embodiments, operation 1540 includes turning off the vacuum applied to the graft storage module. In some embodiments, operation 1540 includes disconnecting one or more of the tubes connected to the graft storage module.
[98] In an operation 1545, the harvested grafts are collected from the graft storage module. In an illustrative embodiment, collecting the harvested grafts includes pouring out the liquid (in which the harvested grafts are suspended) from the storage chamber. The liquid can be poured out into another vessel, such as a tray. In an

alternative embodiment, collecting the harvested grafts includes straining the grafts from the liquid (e.g., via the spatula).
EXAMPLE #1
[99] In an illustrative example, saline is added to a storage chamber of a graft storage module. The cap to the graft storage module is secured to the top of the storage chamber. The cap and the storage chamber are sealed using O-rings that seat in grooves of the storage chamber. The cap and the storage chamber are made of transparent polycarbonate. The cap and the storage chamber are cylindrical in shape. A first hollow tube feature extends along an internal axis from the cap and a second hollow tube feature extends along the internal axis from the storage chamber. Attached to the end of the second hollow tube feature is a filter. The first hollow tube feature and the second hollow tube feature are separated by 10 mm. The inside end surface of the cap and the inside end surface of the storage chamber are 100 mm apart.
[100] A punch is used in a graft extraction module to extract cylindrically shaped grafts that each include at least one hair follicle. The inside diameter of the punch is 1 mm. A graft collection tube is attached to the output of the punch. The inside diameter of the graft collection tube is 1.5 mm. The graft collection tube is attached to a first port of the graft storage module. The inside diameter of the first hollow tube feature (and the second hollow tube feature) is 1.5 mm. The filter at the end of the second hollow tube feature has slits that are 0.5 mm wide. The connector of the graft storage module is connected to a vacuum source. The pressure supplied by the vacuum source is 500 mm Hg.
[101] Hair follicles are harvested from a donor source using the punch. The hair follicles are suctioned away from the punch, through the graft collection tube, and through the first hollow tube feature. The hair follicles fall into the liquid. After hair follicles are collected in the graft storage module, the vacuum pressure is turned off, and the graft collection tube and the vacuum tube are disconnected from the graft storage module. The cap is pulled off of the storage chamber, and the contents of the storage chamber are poured out for implantation of the grafts into a recipient.
EXAMPLE #2
[102] In an illustrative example, a spatula is placed inside of a storage chamber. Saline is added to the storage chamber. The cap to a graft storage module is secured to the top of the storage chamber and the graft storage module is kept in an upright position (i.e., with the cap above the storage chamber). The cap and the storage chamber are sealed using O-rings that seat in grooves of the storage chamber. The cap and the storage chamber are made of transparent polycarbonate. The cap and the storage chamber comprise a cylindrical shape. A first hollow tube feature extends down from the cap and protrudes into a volume defined by the storage chamber. The first hollow tube feature extends below the surface of the saline.

[103] A punch is used in a graft extraction module to extract cylindrically shaped grafts that each include at least one hair follicle. The inside diameter of the punch is 1 mm. A graft collection tube is attached to the output of the punch. The inside diameter of the graft collection tube is 1.5 mm. The graft collection tube is attached to a first port of the graft storage module. The inside diameter of the first hollow tube feature is 1.5 mm. The connector of the graft storage module is connected to a vacuum source. The pressure supplied by the vacuum source is 500 mm Hg. The clinician can adjust the amount of vacuum supplied by the vacuum source to be any pressure less than 760 mm Hg.
[104] Hair follicles are harvested from a donor source using the punch. The hair follicles are suctioned away from the punch, through the graft collection tube, and through the first hollow tube feature. The hair follicles are expelled from the first hollow tube feature into the liquid. After hair follicles are collected in the graft storage module, the vacuum pressure is turned off, and the graft collection tube and the vacuum tube are disconnected from the graft storage module. The spatula is used to strain the grafts from the liquid. The spatula has perforations with diameters of 0.5 mm. The spatula is lifted up and out of the liquid, and the grafts rest on the top surface of the spatula. The grafts are then implanted into a recipient.
[105] The herein described subject matter sometimes illustrates different components contained within, or connected with, different other components. It is to be understood that such depicted architectures are merely exemplary, and that in fact many other architectures can be implemented which achieve the same functionality. In a conceptual sense, any arrangement of components to achieve the same functionality is effectively "associated" such that the desired functionality is achieved. Hence, any two components herein combined to achieve a particular functionality can be seen as "associated with" each other such that the desired functionality is achieved, irrespective of architectures or intermedial components. Likewise, any two components so associated can also be viewed as being "operably connected," or "operably coupled," to each other to achieve the desired functionality, and any two components capable of being so associated can also be viewed as being "operably couplable," to each other to achieve the desired functionality. Specific examples of operably couplable include but are not limited to physically mateable and/or physically interacting components and/or wirelessly interactable and/or wirelessly interacting components and/or logically interacting and/or logically interactable components.
[106] With respect to the use of substantially any plural and/or singular terms herein, those having skill in the art can translate from the plural to the singular and/or from the singular to the plural as is appropriate to the context and/or application. The various singular/plural permutations may be expressly set forth herein for sake of clarity.
[107] It will be understood by those within the art that, in general, terms used herein, and especially in the appended claims (e.g., bodies of the appended claims)

are generally intended as "open" terms (e.g., the term "including" should be interpreted as "including but not limited to," the term "having" should be interpreted as "having at least," the term "includes" should be interpreted as "includes but is not limited to," etc.). It will be further understood by those within the art that if a specific number of an introduced claim recitation is intended, such an intent will be explicitly recited in the claim, and in the absence of such recitation no such intent is present. For example, as an aid to understanding, the following appended claims may contain usage of the introductory phrases "at least one" and "one or more" to introduce claim recitations. However, the use of such phrases should not be construed to imply that the introduction of a claim recitation by the indefinite articles "a" or "an" limits any particular claim containing such introduced claim recitation to inventions containing only one such recitation, even when the same claim includes the introductory phrases "one or more" or "at least one" and indefinite articles such as "a" or "an" (e.g., "a" and/or "an" should typically be interpreted to mean "at least one" or "one or more"); the same holds true for the use of definite articles used to introduce claim recitations. In addition, even if a specific number of an introduced claim recitation is explicitly recited, those skilled in the art will recognize that such recitation should typically be interpreted to mean at least the recited number (e.g., the bare recitation of "two recitations," without other modifiers, typically means at least two recitations, or two or more recitations). Furthermore, in those instances where a convention analogous to "at least one of A, B, and C, etc." is used, in general such a construction is intended in the sense one having skill in the art would understand the convention (e.g., "a system having at least one of A, B, and C" would include but not be limited to systems that have A alone, B alone, C alone, A and B together, A and C together, B and C together, and/or A, B, and C together, etc.). In those instances where a convention analogous to "at least one of A, B, or C, etc." is used, in general such a construction is intended in the sense one having skill in the art would understand the convention (e.g., "a system having at least one of A, B, or C" would include but not be limited to systems that have A alone, B alone, C alone, A and B together, A and C together, B and C together, and/or A, B, and C together, etc.). It will be further understood by those within the art that virtually any disjunctive word and/or phrase presenting two or more alternative terms, whether in the description, claims, or drawings, should be understood to contemplate the possibilities of including one of the terms, either of the terms, or both terms. For example, the phrase "A or B" will be understood to include the possibilities of "A" or "B" or "A and B." Further, unless otherwise noted, the use of the words "approximate," "about," "around," "substantially," etc., mean plus or minus ten percent.
[108] The foregoing description of illustrative embodiments has been presented for purposes of illustration and of description. It is not intended to be exhaustive or limiting with respect to the precise form disclosed, and modifications and variations are possible in light of the above teachings or may be acquired from practice of the disclosed embodiments. It is intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

WHAT IS CLAIMED IS:
1. A device comprising:
a cap that includes a cap inlet port and a cap outlet port in fluid communication with one another, wherein the cap inlet port is configured to engage an inlet tube through which a graft is received, and wherein the cap outlet port is in fluid communication with a graft storage volume that is formed at least in part by the cap; and
a body that is coupled to the cap and that forms at least a portion of the graft storage volume, wherein the body includes a body inlet port and a body outlet port in fluid communication with one another, wherein the body outlet port is configured to engage a vacuum tube to draw the graft into the graft storage volume, and wherein the vacuum tube provides a vacuum pressure that causes a temperature of the graft storage volume to drop below an ambient temperature.
2. The device of claim 1, wherein the graft comprises a hair follicle.
3. The device of claim 1, further comprising a liquid within the graft storage volume that is configured to moisturize the graft.
4. The device of claim 3, wherein a volume of the liquid is a fixed volume that allows the device to be placed in any orientation.
5. The device of claim 3, wherein the liquid comprises saline.
6. The device of claim 1, further comprising a filter on an end of the body inlet port that is configured to prevent the graft from entering the body inlet port.
7. The device of claim 6, wherein a hole size of the filter is no greater than a diameter of the graft.
8. The device of claim 7, wherein the diameter of the graft is a minimum diameter of the graft.
9. The device of claim 7, wherein the hole size of the filter is no greater than one half of the diameter of the graft.
10. The device of claim 1, wherein at least a portion of the body is transparent.
11. The device of claim 1, wherein at least a portion of the body is made of polycarbonate.
12. The device of claim 1, wherein the cap inlet port and the cap outlet port are centered on the cap, and wherein the body inlet port and the body outlet port are centered on the body.
13. The device of claim 1, wherein the cap outlet port is mounted to the cap at a first end of the cap outlet port, wherein a second end of the cap outlet port is centered along a central axis of the body, wherein the body inlet port is mounted to the body at a first end of the body inlet port, and wherein a second end of the body inlet port is centered along the central axis of the body.
14. The device of claim 1, wherein an inside diameter of the cap outlet port is the same size or larger than an inside diameter of the inlet tube.
15. The device of claim 1, further comprising a clip mounted on an outer surface of either the cap or the body, wherein the clip is configured to attach to a graft harvesting device.

16. The device of claim 15, wherein the clip is removably mounted on the outer surface of either the cap or the body.
17. The device of claim 1, wherein the cap outlet port comprises an inner surface that is lubricated.
18. The device of claim 17, wherein the inner surface of the cap outlet port is either hydrophobic or hydrophilic.
19. A device comprising:
a cap that includes a first inlet port and a first outlet port in fluid communication with one another, wherein the first inlet port is configured to engage an inlet tube through which a graft is received, wherein the cap further includes a second inlet port and a second outlet port in fluid communication with one another, wherein the second outlet port is configured to engage a vacuum tube to draw the graft into a graft storage volume, wherein the vacuum tube provides a vacuum pressure that causes a temperature of the graft storage volume to be less than an ambient temperature, and wherein the first outlet port is in fluid communication with the graft storage volume that is formed at least in part by the cap; and
a body that is coupled to the cap and that forms at least a portion of the graft storage volume.
20. The device of claim 19, further comprising a filter in the second inlet port that prevents the graft from traveling through the second outlet port.
21. The device of claim 20, wherein the filter does not extend beyond an inner surface of the cap.
22. The device of claim 19, further comprising a strainer that includes a perforated end piece that fits within the portion of the graft storage volume formed by the body and that includes a handle extending from the perforated end piece.
23. The device of claim 22, wherein a diameter of perforated holes of the perforated end piece is smaller than a diameter of the graft.
24. The device of claim 20, further comprising a liquid within the graft storage volume that is configured to moisturize the graft.
25. The device of claim 24, wherein the first outlet port extends from the cap into the liquid.
26. A method comprising:
inserting a volume of liquid into a graft storage module that has an inlet port and a vacuum port, wherein the liquid is configured to moisturize a graft;
attaching a vacuum tube to the vacuum port;
applying, via the vacuum tube, a suction to the graft storage module that causes a temperature of the liquid to be less than an ambient temperature; and
causing, using the suction, the graft to enter into the graft storage module via the inlet port of the graft storage module.
27. The method of claim 26, further comprising removing the graft from the graft storage module.
28. The method of claim 27, further comprising removing a cap of the graft storage module from a body of the graft storage module.
29. The method of claim 27, wherein said removing the graft comprises pouring the volume of liquid and the graft out of the graft storage module.

30. The method of claim 27, wherein said removing the graft comprises filtering the graft from the liquid.
31. The method of claim 30, wherein said filtering comprises lifting a perforated spatula from a bottom of the graft storage module.
32. The method of claim 26, further comprising securing a cap of the graft storage module to a body of the graft storage module.
33. The method of claim 26, wherein the volume of the liquid is maintained upon application of the suction to the graft storage module.
34. The method of claim 26, further comprising mounting the graft storage module to a harvesting device.
35. The method of claim 34, wherein said mounting the graft storage module comprises attaching a clip that is attached to the graft storage module to a body section of the harvesting device.
36. The method of claim 34, wherein said mounting the graft storage module comprises connecting the inlet port of the graft storage module to an outlet port of the harvesting device.