Description:TECHNICAL FIELD
[1] The present invention discloses a bio stimulant and a process of preparing a bio stimulant.
BACKGROUND OF THE INVENTION
[2] Bio stimulants are products that reduce the need for fertilizers and increase plant growth, resistance to water, biotic and abiotic stresses. In small concentrations, these substances are efficient, favouring the good performance of the plant’s vital processes, and allowing high yields and good quality products. In addition, bio stimulants applied to plants enhance nutrition use efficiency, plant quality traits, regardless of its nutrient contents.
[3] Unlike traditional fertilisers or pesticides, bio stimulants are not a plant growth regulator. In this perspective, using bio stimulants is seen as a cutting-edge, environmentally responsible sustainable agricultural technique. As of right now, bio stimulant products have a major impact on the worldwide farming sector and are expected to continue to grow in the years to come. Recent discoveries state that minerals such as zinc, boron, molybdenum, manganese, copper, cobalt, magnesium, or calcium, are needed nutrients that combine with bio stimulants.
[4] In many crops, particularly but not exclusively horticulture, the use of bio stimulants may lessen the need for chemical fertiliser application since they provide away to improve crop nutrition. Plant bio stimulants are widely used in a variety of cropping systems, including integrated and conventional crop production, although they were originally developed for organic farming. Commercial protein-based bio stimulants are primarily derived from naturally occurring vegetable plant sources, although they can also be made with animal proteins if desired functionalities are desired (e.g. enhancing plant biotic/abiotic tolerances).
[5] In addition to foliar fertilizers containing nitrogen, phosphorus, potassium, and micronutrients, the recently developed patents RO 103652, RO 95689, RO 116080, RO 116081, RO 116189, US 5302180, and US 7198805 also combine several natural or synthetic organic substances, such as plant extracts, protein hydrolysates or glycoproteins, naphthanates, polycarboxylic acids, and humic substances, to promote nutrient metabolism and aid in the adsorption and penetration by leaves of the nutritional species. According to this, the liquid complex fertiliser compositions described in patents RO 127192 and RO 127894 contain organic materials and disodium salt of EDTA along with humic substances containing total nitrogen, phosphorus, potassium, and micronutrients like iron, zinc, copper, manganese, boron and magnesium.
[6] Bio stimulants are used to treat crops because of their capacity to boost photosynthetic rate, reduce growth rates, raise stress tolerances, and increase disease resistance. There's still a need for better solutions that can meet a range of applications, even with the abundance of commercially available plant bio stimulants and fertilisers. Plant bio stimulants, which increase a plant's tolerance to stresses, are especially needed in the turf grass, horticulture, and agriculture industries. A few examples of stresses including salinity, heat, alkalinity, drought, and excess moisture. Additionally, there is a need for plant bio stimulants that work better when used less frequently than currently available commercial solutions. Moreover, plant biostimulants that provide a reduced risk of plant damage are necessary in the case of overapplication. Reducing fertiliser leaching into the environment also required parameter for improving food crops' nutrient usage efficiency. In addition to improving plant tolerance to a variety of abiotic stresses, the use of natural plant bio stimulants to boost flowering, plant growth, fruit set, crop output, and efficient nutrient utilisation is a promising and environmentally beneficial innovation.
[7] Accordingly, it is a technical challenge to provide a bio stimulant that increase a plant’s reaction to stresses and that has a lower risk of plant harm

SUMMARY
[8] In one embodiment, a bio stimulant for enhancing crops grown in horticulture and agriculture is disclosed. The composition of bio stimulant comprises various kinds of active nitrogenous organic compounds including heterocyclic nitrogenous compounds (triazine, triazole, diazine, tetrazine, tetrazole), cynamides (i.e., dicyanamide), amines (i.e., ethylene diamine, ethylene triamine, hexamine), imines, amino alcohols (i.e., mono ethanolamine, diethanolamine and triethanolamine), polymeric nitrogenous compounds, adenine and guanine interacted with the combination of bulkier multi-functional organic acids such as humic, fulvic, folic, ascorbic, gluconic, polyglycolic, citric, tartaric, oxalic, lactic, polylactic, thiazoles, succinic, 9-oxononanoic, arachidonic, pipecolic, salicylic, phosphonic, gallic, maleic, polymaleic, adipic, tannic, alginic, glycemic, barbituric, thiosalicylic, ascorbic acid, polyascorbic, polytartaric, cinnamic, jasmonic, lipoic acid, glutathione, betainglycine, specific or combination of amino acids from plant sources and animal sources as well as nucleic acids produces the novel bio-stimulants.
[9] In another embodiment, a method of formulating a bio stimulant is disclosed. The bio stimulant comprises heterocyclic nitrogenous compounds (triazine, triazole, diazine, tetrazine, tetrazole), cynamides (i.e., dicyanamide), amines (i.e., ethylene diamine, ethylene triamine, hexamine), imines, amino alcohols (i.e., mono ethanolamine, diethanolamine and triethanolamine), polymeric nitrogenous compounds, adenine and guanine interacted with the combination of bulkier multi-functional organic acids such as humic, fulvic, folic, ascorbic, gluconic, polyglycolic, citric, tartaric, oxalic, lactic, polylactic, thiazoles, succinic, 9-oxononanoic, arachidonic, pipecolic, salicylic, phosphonic, gallic, maleic, polymaleic, adipic, tannic, alginic, glycemic, barbituric, thiosalicylic, ascorbic acid, polyascorbic, polytartaric, cinnamic, jasmonic, lipoic acid, glutathione, betainglycine, specific or combination of amino acids from plant sources and animal sources as well as nucleic acids produces the novel bio-stimulants.

DETAILED DESCRIPTION
[10] This disclosure is related to the field of chemical bio stimulants, which are substances used in agriculture to encourage plant growth. The disclosure specifically pertains to the combination of organic nitrogenous chemicals on interaction with multifunctional organic acids employed as a biostimulant to promote plant growth development. A crucial factor in determining a plant's early growth and good development in the first few weeks is the root system's quickest rate of nutrient uptake. Enhancing plant development is made possible by biostimulants, which either supply nutrients derived from natural sources or facilitate plant uptake of these nutrients.
[11] The terms "bio stimulating" or "bio stimulant" describes the capacity of the compositions to promote the healthy growth of a certain plant, bacterium, or other living microorganism. The disclosed bio stimulants help plants to grow better by increasing the nutrient use efficiencies and activating the plant bio-enzymatic system to boost the plant growth as well as biotic/abiotic stress tolerances.
[12] Typically, bio stimulants boost the efficiency of plant metabolism, which results in a higher yield and higher quality of crop. They also encourage plant growth and development throughout the whole life cycle of the crop, from seed germination to plant maturity. In further, the plants' capacity to withstand and bounce back from abiotic challenges is improved. In further, the biostimulant formulation mainly helps the plant to improve their nutrient use efficiencies and biological metabolisms. The bio stimulant disclosed herewith enhances the sugar content, protein content, chlorophyll content, colour, flower and fruit size of agricultural products. They also control and enhance plants' water content. Eventually, these biostimulants improve a few of the soil's physicochemical characteristics particularly encourage the growth of microorganisms as well as maintaining the soil pH of the field.
[13] In fact, by demonstrating the stimulating effect of a formulation of the disclosed biostimulants, a combination of organic nitrogenous chemicals compounds with bulkier high carbon-containing multifunctional acid on plant development is disclosed. A composition comprising at least one multifunctional organic acid that is connected with an organic nitrogenous component is disclosed. The disclosed biostimulants improves primary and secondary photosynthesis by use of mixture of synthetic and natural multifunctional organic acid molecules that coordinates with organic nitrogenous substances.
[14] The feedstocks for the disclosed biostimulants include extracts from leaves, roots, or seeds, either alone or in combination with other materials. Plant growth-promoting bioactive substances such as phytohormones, amino acids, and minerals are primarily responsible for the beneficial impact on horticulture production. Particularly, the disclosed biostimulant enhances tolerance of abiotic and biotic stresses, plant growth and yield, nutrient usage efficiency, and quality attributes of the crop and product (at harvest and beyond).
[15] The synthesis of a bio stimulant comprises a combination of bulky, highly carbonised, multi-functionalized organic acid and nitrogenous compounds. The bio stimulants have improved the rate of fertiliser usage while stimulating the growth of plant roots, thus maximising the goal of volume increase.
[16] The combination of multi bulkier carbogenous organic acids interacting or bonded with active nitrogenous chemical compounds, is synergistic and desired for crop growth and development. In order to address the needs of crops, this type of bio stimulant provides efficient utilization of nutrients by offering a multi-nutritive solution that improves crop uptake in a single application.
[17] Additionally covered by the invention is a method of treating plants, seeds, crops, plant propagation material, locus, parts thereof, or soil with an agricultural composition that includes a minimum of one organic multifunctional acid and its polymers derivatives or mixtures with the combination of an effective amount of nitrogenous compounds and at least one excipient that is acceptable in terms of agrochemistry. The compositions are applied to the soil in a very favourable way by drilling, soaking, disseminating, bending or side placing, or micro irrigation with drip or trickle systems.
[18] An embodiment states that the agricultural content can be either liquid or solid. The agricultural component, for instance, could take the shape of water-dispersible granules, soluble liquid, soluble powder, gel, fortified suspension, aqueous suspension, suspo-emulsion, suspension concentrate, aqueous dispersion, seed dressings, or emulsions for seed treatment, soil application, foliar application, drone application and mixtures of these states.
[19] The composition's organic nitrogenous substance and organic multifunctional acid serve as fertilizers as well as aid in lowering the pH of the soil and promoting improved absorption of other nutrients and amino acids. The synergistic effects of adding nitrogenous compound and organic multifunctional acid, as well as their polymers, salts, derivatives, or mixtures thereof, to water-dispersible granules, water soluble liquid, powder, granules or liquid suspensions with particle sizes ranging from 0.01 to 20 microns, demonstrated superior field efficacy in terms of crop growth and development. The separate application of nitrogenous material and multifunctional acids did not yield the same outstanding efficacy.
[20] An embodiments states that the composition of various kinds of active nitrogenous organic compounds including heterocyclic nitrogenous compounds (triazine, triazole, diazine, tetrazine, tetrazole), cynamides (i.e., dicyanamide), amines (i.e., ethylene diamine, ethylene triamine, hexamine), imines, amino alcohols (i.e., mono ethanolamine, diethanolamine and triethanolamine), polymeric nitrogenous compounds (i.e., polyamines), adenine and guanine.
[21] An embodiment states that the composition of bulkier multi-functional organic acids such as humic, fulvic, folic, ascorbic, gluconic, polyglycolic, citric, tartaric, oxalic, lactic, polylactic, thiazoles, succinic, 9-oxononanoic, arachidonic, pipecolic, salicylic, phosphonic, gallic, maleic, polymaleic, adipic, tannic, alginic,polyalginic,glycemic, barbituric, thiosalicylic, ascorbic acid, polyascorbic, polytartaric, cinnamic, jasmonic, lipoic acid (LA), glutathione (GSH), betainglycine, specific or combination of amino acids from plant sources and animal sources as well as Nucleic Acids produces the bio-stimulants.
[22] An embodiment states that the composition includes one or more amino acids at minimum. The amino acids can contain one or more of the following: peptides, plant and animal proteins, hydrolyzed proteins, polyamino acids, salts or derivatives thereof, or mixes thereof. Essential, non-essential, and non-proteogenic amino acids can also be included. The amino acids include but are not limited to, the following: proline, phenylalanine, serine, selenocysteine, valine, taurine, tyrosine, theanine, threonine, tryptophan, tyramine, glutamic acid, glutamine, glycine, Gaba-aminobutyric acid, histidine, methionine, ornithine, leucine, lysine, isoleucine, cysteine, and so on. A variety of proteins, including but not limited to soy protein, whey protein, fig protein, fish protein, pea protein, maize protein, casein protein, sunflower protein, barley protein, egg protein, rapeseed protein, rice protein, gelatin, chicken protein, or combinations of these, are considered hydrolysed protein. Protein hydrolysates may originate from microbial, animal, or vegetable sources. Vegetable, animal, or microbial sources can provide hydrolyzed proteins. One or more of the following polymers are made up of five amino acids: polylysine, polyornithine, polyarginine, polylactic acid, polyglutamic acid, polylactide, polythreonine, polyproline, polyhistidine, and polytyrosine. Nonetheless, experts in the field will recognize that alternative amino acids can be employed without deviating from the current invention's parameters. Commercially synthesized amino acids, their polymer salts, derivatives, peptides, and protein hydrolysates can be obtained from a variety of companies.
[23] An embodiment states that the micronutrients consist of one or more of the following; elemental forms of zinc, boron, iron, copper, manganese, silicon, cobalt, molybdenum, selenium, nickel, and chlorine; or there salts, complexes, derivatives, or mixtures thereof can be used to further improve the bio-efficacy of newly developed bio stimulant. But the micronutrient list above is not intended to be an exhaustive list; rather, it is meant to serve as an example.
[24] An embodiment states that the micronutrients consist of one or more of the following: elemental forms of calcium, magnesium and sulphur or their salts, complexes, derivatives, or mixtures thereof can be used to further improve the bio-efficacy of newly developed bio stimulant. But the secondary nutrient list above is not intended to be an exhaustive list; rather, it is meant to serve as an example.
[25] An embodiment state that the anionic surfactants consist of one or the combination of the following but not limited to a salt of fatty acid, benzoate, polycarboxylate, ester of alkyl sulfuric acid, alkyl ether sulphates, alkyl sulphate, alkyl diglycol ether sulphate, salt of alcohol sulfuric acid ester, alkyl sulfonate, alkylaryl sulfonate, aryl sulfonate, lignin sulfonate, alkyl diphenyl ether disulphonate, polystyrene sulfonate, salt of alkyl phosphoric acid ester, alkyl aryl phosphate, styryl aryl phosphate, sulfonate docusates, salt of polyoxyethylene alkyl ether sulfuric acid ester, polyoxy ethylene alkyl aryl ether sulphate, alkyl sarcosinates, alpha olefin sulfonate sodium salt, alkyl benzene sulfonate or its salts, sodium lauryl sarcosinate sulfosuccinates, polyacrylates, polyacrylate free acid and sodium salts, salt of polyoxy ethylene alkyl aryl ether sulfuric ester, polyoxyethylene alkyl ether phosphate, salt of polyoxy ethylene alkyl aryl phosphoric acid ester, sulfosuccinates -mono and other diesters phosphate esters, alkyl nathphalene sulfonate -isopropyl and butyl derivatives, alkyl ether sulphates sodium and ammonium salts; ethylene oxides and its derivatives, a salt of mono-alkyl sulphosuccinates, aromatic hydrocarbon sulphonates, 2-acrylamido-2-methyl propane sulfonic acid, ammonium lauryl sulphate, ammonium perfluorononate, docusate, disodium cocoamphodi acetate, magnesium laurethsulfate, perfluoro burane sulfonic acid, perfluro nonanoic acid, carboxylates, perfluoro octane sulfonic acid, perfluoro octanoic acid, phospholipid, potassium lauryl sulphate, sodium alkyl sulphate, sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, sodium laurate, sodium laurethsulphate, sodium lauroylsarcocinate, sodium myrethsulphate, sodium nonanoyl oxybenzene sulfonate, alkyl carboxylates, sodium stearate, alpha olefin sulphonates, naphthalene sulfonate salts, alkyl naphthalene sulfonate fatty acid salts, naphthalene sulfonate condensates sodium salt. Fluoro carboxylate, fatty alcohol sulphates, alkyl naphthalene sulfonate condensates sodium salt, naphthalene sulfonic acid condensed with formaldehyde, or salt of alkyl naphthalene sulfonic acid condensed with formaldehyde or salts derivatives thereof.
[26] In another embodiment, cationic surfactants comprise one or more of the following but not limited to dialkyl dimethyl ammonium chloride, alkyl methyl ethoxylated ammonium chlorides or their salts, dodecyl-, coco-, hexadecyl-, octadecyl-, octadecyl/behenyl-, behenyl-, cocoamidopropyl-, trimethyl ammonium chloride; stearyl-, bis(2-hydroxy ethyl) methyl ammonium chloride, benzalkonium chloride, alkyl-, tetradecyl-, octadecyl-dimethyl benzyl ammonium chloride, dioctyl-, di(octyl-decyl)-, dihexadecyl-, distearyl-, di(hydrogenated tallow) dimethyl ammonium chloride, di(hydrogenated tallow) benzyl-, triocyl-, tri(octyl-decyl)-, tridodecyl-, trihexa decyl-methyl ammonium chloride, dodecyl trimethyl-, dodecyl dimethyl benzyl-, di(octyl-decyl) dimethyl, dodecyl dimethyl-ammonium bromide, quaternised amine ethoxylates, behentrimonium chloride, benzethonium chloride, benzododecinium bromide, bronidox, quaternary ammonium salts carbethopendecinium bromide, cetalkonium chloride, certrimonium bromide, certimonium chloride, cetylpyridinium chloride, didecyldimethyl ammonium chloride, lauryl methyl gluceth, hydroxy propyl dimonium chloride, octenidine dihydro chloride, olaflur, N-oleyl-1, 3-propane diamine, pahutoxin, stearalkonium chloride, tetramethyl ammonium hydroxide, thonzonium bromide; salts or derivatives thereof.
[27] Another embodiment, states that the non-ionic surfactants consist of among other things, one or more of polyol esters, polyol fatty acid esters, polyethoxylated esters, polyethoxylated alcohols, ethoxylated and propoxylated fatty alcohols, ethoxylated and propoxylated alcohols, EO/PO copolymers; EO and PO block copolymers, di, tri-block copolymers; block copolymers of polyethylene glycol and polypropylene glycol, poloxamers, polysorbates, alkyl polysaccharides such as alkyl poly glycosides and blends thereof, amine ethoxylates, sorbitan fatty acid ester, glycol and glycerol esters, glucosidyl alkyl ethers, sodium tallowate, poly oxyethylene glycol, sorbitan alkyl esters, sorbitan derivatives, fatty acid esters of sorbitan and their ethoxylated derivatives (Tweens), and sucrose esters of fatty acids, cetostearyl alcohol, cetyl alcohol, cocamide DEA, cocamide MEA, decyl glucoside, decyl polyglucose, glycerol monostearate, lauryl glucoside, maltosides, monolaurin, narrow-range ethoxylate, nonidet-40, nonoxynol-9, nonoxynols, octaethylene glycol mono dodecyl ether, N-octyl beta-D-thioglucopyranoside, octyl glucoside, oleyl alcohol, PEG-10 sunflower glycerides, pentaethylene glycol mono dodecyl ether, polidocanol, poloxamer, polyethoxylated tallow amine, polyglycerol polyricinoleate, polysorbate, polysorbate 20, polysorbate 80, sorbitan, sorbitan monolaurate, sorbitan monostearate, sorbitantri stearate, stearyl alcohol, surfactin, glyceryl laureate, lauryl glucoside, nonyl phenol poly ethoxy ethanols, nonyl phenol polyglycol ether, castor oil ethoxylate, polyglycol ethers, polyadducts of ethylene oxide and propylene oxide, block copolymer of poly alkylene glycol ether and hydroxy stearic acid, tributyl phenoxy polyethoxy ethanol, octyl phenoxy polyethoxy ethanol, etho-propoxylatedtristyrl phenols, ethoxylated alcohols, polyoxy ethylene sorbitan, fatty acid poly glyceride, a fatty acid alcohol polyglycol ether, acetylene glycol, acetylene alcohol, oxyalkylene block polymer, polyoxy ethylene alkyl ether, polyoxy ethylene alkyl aryl ether, polyoxy ethylene styrylaryl ether, polyoxy ethylene glycol alkyl ether, poly ethylene glycol, polyoxy ethylene fatty acid ester, polyoxy ethylene sorbitan fatty acid ester, a poly oxyethylene glycerin fatty acid ester, alcohol ethoxylates-C6 to C16/18 alcohols, linear and branched, alcohol alkolylates- various hydrophobes and EO/PO contents and ratios, fatty acid esters-mono and diesters; lauric, stearic and oleric; glycerol esters- with and without EO; lauric, stearic, coco and tall oil derived, ethoxylated glycerine, sorbitan esters with and without EO; lauric, steric and oleic based; mono and trimesters, castor oil ethoxylates- 5 to 500 moles EO; non-hydrogeneted and hydrogenated, block polymers, amine oxides-ethoxylated and non-ethoxylated; alkyl dimethyl, fatty amine ethoxylates-coco, tallow, stearyl, oleyl amines, polyoxy ethylene hydrogenated castor oil or polyoxy propylene fatty acid ester; salts or derivatives thereof.
[28] An embodiment state that the amphoteric or zwitterionic surfactants consist of one or more of the following, but not only of these: betaine, coco and lauryl amidopropyl betaines, coco alkyl dimethyl amine oxides, alkyl dimethyl betaines; C8 to C18, alkyl dipropionates sodium laurimino dipropionate, cocoamido propyl hydroxyl sulfobetaine, imidazolines, phospholipids phosphatidylserine, phosphatidyl ethanolamine, phosphatidyl choline, and sphingomyelines, lauryl dimethylamine oxide, alkyl amphoacetates and proprionates, alkyl ampho(di)acetates, and di-proprionates, lecithin and ethanolamine fatty amides; or salts derives thereof.
[29] The surfactants that are marketed under the name and may be purchased commercially include one or more of the surfactants that are marketed under the name and may be purchased commercially include one or more of Altas G5000, Atlas PN 100, Termul 5429, Termul 2510, Ecoteric@, Eulsogen@118, Genapol@X, Genapol@OX, Genapol@C, Emulsogen@EL, Arlacel P135, Hypermer 8261, Hypermer B239, Hypermer B261, Hypermer B246sf, Solutol HS, Promulgen TM D, Soprophor 7961P, Soprophor TSP/461, Soprophor TSP/724, Croduret 40, Etocas 200, Etocas 29, Rokacet R26, Cetomacrogol 1000, Chemonic OE-20, Triton N20, Triton X-100, Tween 20, 23, 40, 60, 65, 80, Span 20, 40, 60, 80, 83, 85, 120, Brij@, Atlox 4912, Atlas G5000, Termul 3512, Termul 3015, Termul 5429, Termul 2510, Ecoteric@, Ecoteric@ T85, Ecoteric@T20, Teric 12A4, Eulsogen@118, Genapol@X, Genapol@OX-080, Genapol@C 100, Emulsogen@EL 200, Arlacel P135, Hypermer 8261, Hypermer B239, Hypermer B261, Hypermer B246sf, solutol HS, Promulgen TM D, Soprophor 7961P, Soprophor TSP/461, Soprophor TSP/724, Croduret 40, Etocas 200, Etocas 29, Rokacet R26, Chemonic OE-20, Triton TM N-101, Igepal CA630 and Isoceteth-20.In accordance with the embodiments, the surfactant makes up between 0.1 and 40 percent w/w of the composition overall based on the type and composition of the formulation.
[30] An embodiment states that water-miscible solvents are among the solvents utilized in the agricultural compound. The water miscible solvents include but are not limited to 1, 4-dioxane, monoethylene glycol, diethylene glycol, glycerol, N-methyl-2-pyrrolidone, 1,3-propanediol, 1,5-pentanediol, propylene glycol, polyethylene glycol, triethylene glycol, 1,2-butanediol, 1,3-butanediol, 1,4-butanediol, dimethyl formamide, dimethoxy ethane, dimethyl octanamide, dimethyl decanamide. It is feasible to use alternative water-miscible solvents without going outside the scope of the present invention, as those knowledgeable in the art will recognize. An embodiment states that the solvent makes up between 0.1% and 80% w/w of the entire composition.
[31] An embodiment states that one or more inorganic water-soluble salts are among the disintegration agents utilized in the agricultural composition e.g. Sodium chloride, nitrate salts, water-soluble organic compounds such as agar, hydroxypropyl starch, carboxy methyl starch ether, tragacanth, gelatin, casein, microcrystalline cellulose, cross-linked sodium carboxy methyl cellulose, carboxy methyl cellulose, carboxy methyl cellulose calcium, sodium tripolyphosphate, sodium hexametaphosphate, metal stearates, cellulose powder, dextrin, methylacrylate copolymer, polyplasdone@ XL-10 (cross-linked polyvinyl pyrrolidone), poly (vinyl pyrrolidone), sulfonated styrene-isobutylene maleic anhydride copolymer, salts of polyacrylates, salts of methacrylates, starch polyacrylonitrile graft copolymer, sodium of potassium bicarbonates/carbonates or their mixture or salts with acids such as citric and fumaric acid of salts, derivatives thereof. On the other hand, the fact that other disintegration agents can be used without going outside the parameters of the current innovation would be appreciated by experts in the field. The disintegration agents can be purchased from a number ofcompanies and are produced commercially. As per one of the embodiments, the hydrophobic agents comprise one or more of the following: fluorinated silanes, bentonite, attapulgite, talc, hydrophobically modified silicates, and modified starch. The fact that various hydrophobic agents can be used without going outside the parameters of the current invention, however, will be appreciated by those with skill in the field. The hydrophobic ingredient is contained in the composition at concentrations ranging from 0.1 percent to 50 percent w/w, according to one embodiment.
[32] As per embodiment, the binder or binding agents employed in the agricultural composition consist of but are not limited to one or more proteins, lipoproteins, lipids, glycolipid, glycoprotein, carbohydrates such as monosaccharides, disaccharides, oligosaccharides and polysaccharides, complex organic substance, synthetic organic polymers or derivatives and combinations thereof. Conversely, the fact that various binding agents can be used without going outside the parameters of the current invention will be appreciated by those with experience in the field. The binding agents are produced commercially and sold by several companies. One embodiment states that the binding agent makes up between 0.1 and 50 percent w/w of the mixture.
[33] An embodiment states that one or more solid carriers, fillers, or diluents are among the carriers utilized in the agricultural composition. Another embodiment lists plant, synthetic chemicals, mineral, and water-soluble carriers among the carriers. The fact that several carriers can be used without going outside the scope of the current invention, however, will be appreciated by those with skill in the field. The carriers are offered by a number of companies produced commercially. The natural minerals included in the solid carriers are china clay, acid clay, kaolin such as kaolinite, dickite, nacrite, and halloysite, serpentines namely chrysotile, lizardite, antigorite, and amesite, synthetic and diatomaceous silicas, montmorillonite minerals such as sodium montmorillonite, smectites, such as saponite, hectorite, sauconite, and hyderite, micas, such as pyrophyllite, talc, agalmatolite, muscovite, phengite, sericite, and illite, silicas such as cristobalite and quartz, such as attapulgite and sepiolite; vermiculite, laponite, pumice, bauxite, hydrated aluminas, perlite, limestone, natural and synthetic silicates, charcoal, silicas, wet process silicas, dry process silicas, calcined products of wet process silicas, surface-modified silicas, zeolite, diatomaceous earth, derivatives thereof; Chalks (omya), fuller’s earth, mirabilite, white carbon, slaked lime, synthetic silicic acid, starch, modified starch, cellulose, plant carriers such as cellulose, chaff, wheat flour, starch, rice bran, wheat bran, and soyabean flour, tobacco powder, a vegetable powder polyethylene, polypropylene, poly(vinylidene chloride), methyl cellulose, hydroxypropyl cellulose, hydroxy propyl methylcellulose, sodium carboxy methyl cellulose, propylene glycol alginate, polyvinyl pyrrolidone, carboxy vinyl polymer, casein sodium, sucrose, salt cake, potassium pyrophosphate, sodium tripoly phosphate, maleic acid, fumaric acid and malic acid or derivatives or mixtures thereof. The commercially available silicates include Aerosil brands, Sipmat brands as Sipernat@50S and Calflo E and Kaolin. It is conceivable to use alternative solid carriers without going outside the scope of the present invention, as those versed in the art will recognize. An additional embodiment states that the carrier makes up between 0.1% and 80% w/w of the composition based on the type and chemical composition of the formulation.
[34] An embodiment states that the following anti-caking chemicals are included in the agricultural composition: but not limited to one or more polysaccharides such as starch, mannose, galactose, poly(vinyl pyrrolidone), fumed silica (white carbon), ester gum, petroleum resin, Foam master@, Soap L sodium stearate, brij@700, polyoxy ethylene stearyl ether, aerosol@OT-B sodium dioctyl sulfosuccinate, silwet@408, silwet@ L-77 silicone polyether copolymer, sodium acetate, sodium metasilicate, sodium alkyl sulfosuccinates, salts or derivatives thereof. The fact that various anti-caking compounds can be used without going outside the parameters of the current invention, however, will be appreciated by individuals with artistic talent. The anti-caking agents are produced commercially and distributed by a number of businesses. An embodiment states that the anti-caking agent makes up between 0.01 percent and 20 percent w/w of the composition overall.
[35] In an embodiment, one or more of the following antifoaming agents or defoamers can be employed in the agricultural composition: silica, siloxane, silicone dioxide, polydimethyl siloxane, alkyl polyacrylates, ethylene oxide/propylene oxide copolymers, polyethylene glycol, silicone oils and magnesium stearate or derivatives thereof. The antifoaming agents of choice are fatty acids, long-chain alcohols, silicone emulsions such as silikon@SRE, wacker or rhodoril@ from rhodia, long-chain alcohols, fatty acids, fluoro-organic compounds.
[36] An embodiment states that both organic and inorganic acids, bases, and their mixes are employed as pH adjusters, buffers, and neutralizing agents in agricultural compositions. Some embodiment states that the organic acids consist of one or more of citric, malic, adipic, fumaric, maleic, succinic, and tartaric acid or salts, derivatives thereof; and mono-, di-, or tribasic salts of these acids or derivatives thereof. Alkali metal compounds consist of one or more of the following but are not limited to hydroxides of alkali metals including sodium hydroxide and potassium hydroxide, carbonates of alkali metals such as sodium carbonate, hydrogen carbonate of alkali metals such as sodium hydrogen carbonate and alkali metal phosphates such as sodium phosphate and a mixture thereof. An embodiment states that the stats of inorganic acids include butare not limited to one or more alkali metal salts such as sodium chloride, potassium chloride, sodium nitrate, potassium nitrate, sodium sulphate, potassium sulphate, sodium mono hydrogen phosphate, potassium mono hydrogen phosphate, sodium dihydrogen phosphate, potassium dihydrogen phosphate and the like. The mixtures of the above also can be used as pH adjusters’ buffers or neutralizing agents. Those with experience in the field will recognize, however, that other commonly used buffers, neutralizing agents, or pH adjusters can be used without going outside the bounds of the current invention. Commercially produced and distributed by a number of businesses are the pH-adjusters, buffers, and neutralizing agents. An embodiment states that the pH adjusters or buffers make up between 0.01 percent and 10 percent weight percent of the overall composition.
[37] According to an embodiment, the spreading agent utilized in the agricultural composition includes one or more of the following, but not exclusively; cellulose powder, dextrin, modified starch, crosslinked poly(vinylpyrrolidone), a copolymer of maleic acid with a styrene compound, methacrylic acid copolymer, ester of polymer consisting of polyhydric alcohol with dicarboxylic anhydride, water soluble salt of polystyrene sulfonic acid, fatty acids, latex, aliphatic alcohols, vegetable oils such as cottonseed, inorganic oils, petroleum distillates, modified trisiloxanes, polyglycol, polyethers, clatharatesor salts or derivatives thereof. But as those knowledgeable in the art would recognize, additional commonly used spreading agents can be used without going outside the current invention's scope. An embodiment states that the spreading agent makes up between 0.1 and 20 percent w/w of the composition overall.
[38] An embodiment states that the sticking agents which are used in the agricultural composition include, but are not limited to one or more paraffin, polyamide resin, polyacrylate, polyoxy ethylene, wax, polyvinyl alkyl ether, alkylphenol-formalin condensate, fatty acids, latex, aliphatic alcohols, vegetable oils such as cottonseed, inorganic oils, petroleum distillates, modified Tri siloxanes, polyglycol, polyether’s, clathrates, synthetic resin emulsion or salts or derivatives thereof. It is feasible to use other commonly used sticking agents without going outside the scope of the present invention, as those knowledgeable in the art will recognize. The sticking agents are produced commercially and sold by a number of companies. An embodiment states that the sticking agent may make up 0.1 percent to 20 percent w/w of the composition overall.
[39] An embodiment states that the stabilizers which are used in the composition of agricultural biostimulants, but not limited to one or more peroxide-based compounds including hydrogen peroxide and organic peroxides, alkyl nitrites such as ethyl nitrite and alkyl glyoxylates such as ethyl glyoxylate, zeolite, antioxidants such as phenolic compounds and phosphoric acid compounds. The use of alternative well-known stabilizers is feasible without going outside the scope of the current invention, as those knowledgeable in the art will recognize. The stabilizers are produced commercially and sold by a number of companies. An embodiment states that the stabilizer makes up between 0.1% and 30% w/w of the composition overall.
[40] In embodiment, the preservatives which are used in the agricultural composition include but are not limited to, one or more bactericides, anti-fungal agents, biocides, anti-microbial agents, and antioxidants. A few non-limiting examples of preservatives are paraben, esters and salts of paraben, propionic acid and its salts, 2,4-hexadienoic acid (sorbic acid) and its salt, formaldehyde and paraformaldehyde, 2-hydroxy biphenyl ether and its salts, inorganic sulphites and bisulphites, sodium iodate, chloro butanol, dehydra acetic acid, formicacid, 1,6 bis(4-amidino-2-bromophenoxy)-n-hexane and its salts, 5-amino-1,3-bis(225 ethyl hexyl)-5-methyl hexahydropyrimidine, 5-bromo-5-nitro-1,3-dioxane, 2-bromal-2-nitropropane-1,3-diol, 2, 4-dichlorobenzyl alcohol, N-(4-chlorophenyl-1,3-dioxane), 2-bromo-2-nitropane-1,3-diol, 2,4-dichloro benzyl alcohol, N-(4-chloro phenyl)-N’-(3,4-dichlorophyl) urea, 4-chloro-m-cresol, 2,4,4’-trichloro-2’-hydroxydipheyl ether, 4-chloro-3,5-dimethyle phenol, 1,1’-methylene-bis(3-(1-hydroxy methyl-2,4-dioximidazolidin-5-yl urea), poly(hexamethylene diguanide) hydrochloride, 2-phenoxyethanol, hexamethylenetetramine, 1-(3-chloroallyl)-3,5,7-triaza-1-azonia adamantane chloride,1-(4-chlorophenoxy)-1-(1-H-imidazole 3,7,1-yl)-3,3-dimethyl-2-butanone, 1,3-bis(hydroxy methyl)-5,5-dimethyl-2,4-imidazolidine dione, benzyl alcohol, octopirox, 1,2-dibromo-2,4-dicyano butane, 2,2’-methylnene bis(6-bromo-4-chlorophenol), bromo chlorophene, dichlorophene, 2-benzyl-4-chlorophenol, 2-chloroacetamide, chlorhexidine, chlorhexidine acetate, chlorhexidine gluconate, chlorhexidine hydrochloride, 1-phenoxypropane 2-ol, N-alkyl (C12-C22) trimethyl ammonium bromide and chloride, 4,4-dimethyl-1,3-oxazolidine, N-hydroxy methyl-N-(1,3-di(hydroxy methyl)-2,5-dioxoimidazolidin-4-yl)-N’-hydroxy methyl urea, 1,6-bis(4-amidinophenoxy)-n-hexane and its salts, glutaraldehyde, 5-ethyl-1-aza-3,7-dioxabicyclo(3,3,0) octane, 3-(4-chlorophenoxy) propane-1,2-diol, Hyamine, alkyl(C8-C18) dimethyl benzyl ammonium chloride, alkyl(C8-C18) dimethyl benzyl ammonium bromide, alkyl (C8-C18) dimethyl benzyl ammonium saccharinate, benzyl hemiformal, 3-iodo-2-propynyl butyl carbamate, sodium hydroxyl methyl amino acetate, cetyltrimethyl ammonium bromide, cetyl pyridinium chloride, and derivatives of 2H isothiazol-3-one, such as alkyl isothiazolones, benzo isothiazolones or 2-methyl-4,5-trimethylene-2H-isothiazol-3-one (MTIT), Cl-C4-alkyl para hydroxy benzoate, dichlorophene, proxel@ from ICI, bacto-100, thimerosal, sodium propinoate, sodium benzoate, propyl paraben, propyl paraben sodium, potassium sorbate, potassium benzoate, phenyl ethyl alcohol, ethyl paraben, methylparaben, butylparaben, benzyl alcohol, benzothonium chloride, benzalkonium chloride, 1,2-benzothiazol-3-one, preventol@, betyl hydroxy toluene, potassium sorbate, iodine containing organic compounds such as 3-bromo-2,3-diiodo-2-propenyl ethyl carbonate, 3-iodo-2-propynyl butyl carbamate, 2,3,3-triiodo allyl alcohol, and parachlorophenyl-3-iodopropargyl formal; benzimidazole compounds and benthiazole compounds such as 2-(4-thiazolyl)benzimidazole and 2- thiocyanomethylthiobenzo-thiazole; triazole compounds such as 1-(2-(2’,4’,3,8 dichlorophenyl)-1,3-dioxolane-2-yl methyl)-1H-1,2,4-triazole, 1-(2-(2’, 4’-dichloro phenyl)-4-propyl-1, 3-dioxolane-2-yl methyl)-1H-1,2,4-triazole, and naturally occurring compounds such as 4-isopropyl tropolone and boraxor salts or derivatives thereof.
[41] One of the embodiments states that the antioxidants used for this formulation but not limited to one or more of imidazole and imidazole based derivatives i.e., urocanic acid, 4,4’-thiobis-t-butyl-3-methylphenol, 2,6-di-t-butyl-p-cresol (BHT), and pentaaerythrityltetrakis [3-(3,5,-di-t-butyl-4-hydroxy phenyl)] propionate; amine antioxidants such as N,N’-di-2-naphthyl-p-phenylene diamine: hydroquinoline antioxidants such as 2, 5-di(t-amyl)hydroquinoline; phosphorus containing antioxidants such as triphenyl phosphate, carbo-tenoids, carotenes (e.g. a-carotene, -carotene, lycopene) and derivatives thereof, lipoic acid and derivatives thereof (e.g. dihydrolipoic acid) aurothio glucose, propylthio uracil and further thio compounds (e.g. thioglycerol, thiosorbitol, thioglycolic acid, thioredoxin, N acetyl, methyl, ethyl, propyl, amyl, butyl, lauryl, palmitoyl, oleyl, y-linoleyl, cholesteryl and glyceryl esters thereof, and salts thereof, dilauryl thiodipropionate, distearyl thiodipropion-ate, thiodipropionicacid and derivatives thereof (esters, ethers, lipids, nucleotides, nucleosides and salts), and sulfoximine compounds (e.g. buthionine sulfoximines, homocysteine sulfomine, buthionine sulfones, penta-, hexa-, heptathioninesulfoximine) in very low tolerated doses, hydroxy acids i.e., citric acid, lactic acid, malic acid, humic acids, gallic esters, unsaturated fatty acids and derivatives, hydroquinone and derivatives thereof, ubiquinone and ubiquinol, and derivatives thereof, ascorbylpalmite, stearate, di-palmitate, acetate, Mg ascorbyl phosphates, diazo, diumascorbyl phosphate and sulfate, potassium ascorbyltocopheryl phosphate, isoascorbic acid and derivatives thereof, the coniferyl benzoate of benzoin resin, rutin, rutinic acid and derivativesd thereof, disodium rutinyl disulfate, dibutyl hydroxy toluene, 4,4-thiobis-6-tert-butyl phenol, 2,6-tert-butyl-4-methylphenol, pentaerythritol-tetrakis-3-(3,5-ditert butyl-4-hydroxyphenyl) propionate, butyl hydroxy anisol, nordihydroguaiacic acid, orderivatives thereof, selenium and selenium derivatives, stilbenes and stilbene derivatives. On the other hand, additional commonly used preservatives can be used without going outside the scope of the present invention, as those knowledgeable in the art will recognize. The preservatives are produced commercially and sold by a number of companies.
[42] An embodiment states that the preservatives, bactericides, biocides, antifungal agents, anti-microbial agents, and antioxidants are contained in the composition in amounts ranging from 0.1 percent to 20 percent weight percent. One embodiment state that thickeners, viscosity modifiers, tackifiers, suspension aids, rheological modifiers, and anti-settling agents are among the structural agents utilized in agricultural compositions. All of these are not exclusive, though. After extended storage, the active ingredient particles are kept from sediment thanks to a structuring agent.
[43] An embodiment states that the aqueous suspension composition’s structuring agent consists of but is not limited to, one or more polymers such as polyacrylics, polyacrylamides, polysaccharides, hydrophobically modified cellulose derivatives, co-polymers of cellulose derivatives, carboxyvinyl or polyvinyl pyrrolidones, polyethylenes, polyethylene oxide, polyvinyl alcohol and derivatives: clays such as bentonite clays, kaolin, smectite, attapulgites, attaclays with high surface area silica and natural gums such as guar gum, xanthan gum, gum arabic, gum tragacanth, rhamsan gum, locust bean gum, carageenan, welan gum, veegum, gelatin, dextrin, collagen; polyacrylic acids and their sodium salts; the polyglycerol ethers of fatty alcohols and polyethylene oxide or polypropylene oxide condensation products and mixtures thereof include ethoxylated alkyl phenols; ethoxylated aliphatic alcohols; ethoxylated fatty acids; ethoxylated anhydrous sorbitol esters, long chain amine and cyclic amine oxides which are non-ionic in basic solutions long chain tertiary phosphine oxide; and long-chain dialkyl sulfoxides, fumed silica, a mixture of fumed silica and fumed oxides; and long-chain dialkyl sulfoxides, fumed silica, mixture of fumed silica and fumed aluminium oxide, swellable polymers, polyamides or its derivatives; polyols such as glycerine, poly(vinyl acetate), sodium polyacrylate, poly(ethylene glycol), phospholipid; stachyose, fructo oligosaccharides, amylose, pectins, alginates, hydrocolloids and mixtures thereof.
[44] The celluloses based compounds such as hemicellulose, carboxy methyl cellulose, ethyl cellulose, hydroxy ethyl cellulose, hydroxyl-methyl ethyl cellulose, hydroxyl ethyl propyl cellulose, methyl hydroxy ethyl cellulose, methylcellulose; starches such, starch acetates, starch hydroxy ethyl ethers, ionic starches, long chain alkyl starches, dextrins, maltodextrin, corn starch, amine starches, phosphates starches, and dialdehyde starches; plant starches such as corn starch and potato starch; other carbohydrates such as pectin, dextrin, amylopectin, xylanglycogen, agar, gluten, alginic acid, hydrocolloids or derivatives thereof. Experts in the field will recognize, though, that other widely used structuring agents can be used without going outside the bounds of the current invention.
[45] An embodiment states that the freezing point depressants or anti-freezing compounds included in the aqueous suspension composition may include one or more of the following polyhydric alcohols such as ethylene glycol, diethylene glycol, dipropylene glycol, propylene glycol, butyrolactone, N,N-dimethyl formamide, glycerol, monohydric or polyhydric alcohols, glycol, ethers, glycol ethers, glycol mono ethers such as the methyl, ethyl, propyl and butyl ether of ethylene glycol, diethylene glycol, propylene glycol and dipropylene glycol, glycol diethers such as methyl and ethyl diethers of ethylene glycol, diethylene glycol and dipropylene glycol or urea, glycerol, isopropanol, propylene glycol, monomethyl ether, di- or tripropylene glycol monomethyl ether or cyclohexanol carbohydrates such as glucose, mannose, fructose, galactose, sucrose, lactose, maltose, xylose, arabinose, sorbitol, mannitol, trehalose, raffinose or derivatives thereof. The fact that various anti-freezing compounds can be used without going outside the scope of the current invention, however, will be appreciated by those with experience in the field. An embodiment states that the anti-freezing agent makes up between 0.1 and 20 percent w/w of the composition overall.
[46] One embodiment states that the penetrant utilized in the composition of the aqueous suspension includes, but is not limited to, one or more of alcohol, glycol, glycolic ether, ester, amine, alkanolamine, amide oxide, quaternary ammonium compound, triglyceride, fatty acid ester, fatty acid ether, N-methyl pyrrolidone, dimethyl formamide, dimethyl acetamide, or dimethyl sulfoxide, polyoxy ethylene trimethyl propane mono oleate, polyoxy ethylene trimethylolpropanedioleate, polyoxyethylene trimethylolpropanetrioleate, polyoxethylene sorbitanmonooleate, polyoxy ethylene sorbitol hexaoleate. Sophisticated practitioners of the art, however, will recognize that alternative penetrants can be used without going outside the current invention's scope. The penetrants can be purchased from several businesses and are produced commercially.
[47] An embodiment states that the humectant is chosen from among, but not restricted to, one or more of polyoxy ethylene/polyoxy propylene copolymers, particularly block copolymers, such as the synperonic PE series of copolymers available from Uniqema or salts, derivatives thereof. Other humectants are propylene glycol, monoethylene glycol, hexylene glycol, butylene glycol, ethylene glycol, diethylene glycol, poly(ethylene glycol), poly(propylene glycol), glycerol and the like; polyhydric alcohol compounds such as propylene glycol ether, derivatives thereof. Also, other humectants include aloe vera gel, and alpha hydroxyl acids such as lactic acid, glyceryl triacetate, honey, lithium chloride, etc. The non- ionic surfactants mentioned above also act as humectants. However, those skilled in the art will appreciate that it is possible to utilize other conventionally known humectants without departing from the scope of the present invention. The humectants are commercially manufactured and available through various companies. The embodiment states that the humectant is present in the range of 0.1% to 20.90% w/w of the total composition.
[48] The composition of the current invention surprisingly has improved physical properties, including less viscosity, pourability, wettability, suspensibility, and ease of handling. It also minimizes material loss during product handling during field application and packaging. Interestingly, even when greater concentration compositions are utilized in the fields, the inventors have also shown that agricultural compositions in the form of liquid suspension and water-dispersible granules exhibit improved dispersion and, ultimately, efficacy.

[49] Method of preparation
A. Take one or more types of organic multifunctional acids and their salts are sequentially placed in an agitator tank in proportion and stir for5 minutes.
B. One or more types of bioactive organic nitrogenous chemical compounds are added to the above mixture and blended for another 15 minutes to make a homogeneous mixture.
C. Subsequently add the required quantity of suitable surfactant (cationic or anionic or nonionic) and stir for 5 minutes.
D. Then add the required quantity of dispersing agents and anticaking agent one by one with 5 minutes interval at constant stirring.
E. Later add fillers and antioxidants one by one with 5 minutes interval at constant stirring.
F. Finally add stabilizing agent, deformer, thickening agents, anti-freezing agents and biocides one by one with 5 minutes interval at constant stirring to make a biostimulant finished product.
G. Additionally, the final substance prepared as previously mentioned is sprayed directly onto the surface of the corps leaf after being diluted 1,000–1,500 times or directly mixed with fertilizers and applied as soil application.
The different embodiments are described below.
Embodiment: 1
[50] The organic multifunctional acids such as fulvic acid (15-25% by weight), folic acid (0.5 to 2.0% by weight) and humic acid (10 to 50% by weight) are blended with nitrogenous compounds of triazine triamine (5 to 30% by weight) and triazole amine (1 to 15% by weight). Then add anionic surfactant of alkyl ether sulfates (2 to 8% by weight), dispersing agent of sodium lignosulphonate based (3 to 7%) and fumed silica 80 (0.25 to 1.0 % by weight) as anticaking agent. Subsequently, add calcium silicate (5 to 20% by weight) as a filler, amine antioxidants such as N,N’-di-2-naphthyl-p-phenylene diamine (1 to 3% by weight), stabilizerand sodium benzoate (0.01 to 0.1 % by weight) as biocide and blend for 10 minutes to make a homogeneous mixture of bio stimulants in the form of SP formulation.
Embodiment: 2
[51] In this embodiment, a granulated bio stimulant was prepared. The organic multifunctional acids such as ascorbic acid (3-5% by weight), glutamic acid (5 to 20% by weight) and humic acid (20 to 60% by weight) are blended with bioactive nitrogenous compounds of dicyanamide (5 to 20% by weight) and polyamine (10 to 40% by weight). Then add naphthalene condensate (4 to 10% by weight) and precipitated silica (0.25 to 1.0 % by weight). Subsequently, add bentonite (5 to 20% by weight) as a filler, silicon based defoamer (0.1 to 0.25%) and sodium benzoate (0.01 to 0.01 % by weight) and blend for 10 minutes to make a homogeneous mixture. Then add the required amount of water to make a semi-solid material which will be suitable for granulation. This material would be feed into a granulation machine and before that, the required size of the granules (3 to 5 mm) would be fixed. Finally, dry this granulated form of bio stimulant and can be applied to the field with fertilizer or alone depending on the crops.
Embodiment: 3
[52] In this embodiment, a liquid bio stimulant was prepared. The organic multifunctional acids such as poly tartaric acid (10-20% by weight), jasmonic acid (0.5 to 2.0% by weight) and glutathione (10 to 20% by weight) are dissolved in the required quantity of water and followed by add nitrogenous compounds of glycine betaine (5 to 10% by weight) and polyamine (5 to 25% by weight). Then add alkyl methyl ethoxylated ammonium chlorides (2 to 6% by weight) and PEG (2 to 10 % by weight). Subsequently, add xantham gum (2% solution, 5 to 20% by weight) as a suspending agent, antioxidant urocanic acid (1 to 5%) and sodium benzoate (0.01 to 0.1 % by weight) and stir it for 30 minutes to make a homogeneous liquid mixture of bio stimulants in the form of soluble liquid formulation.
Embodiment: 4
[53] In this embodiment, a liquid bio stimulant was prepared. The organic multifunctional acids such as pipecolic (10-20% by weight), alginic acid (0.5 to 2.0% by weight) and barbituric acid (10 to 20% by weight) are dissolved in the required quantity of water and followed by nitrogenous compounds of imines (5 to 10% by weight) and guanine (5 to 25% by weight). Then add alkyl methyl ethoxylated ammonium chlorides (2 to 6% by weight) and PG (2 to 10 % by weight). Subsequently, add xantham gum (2% solution, 5 to 20% by weight) as a suspending agent, antioxidant triphenyl phosphate (1 to 4% by weight) and sodium benzoate (0.01 to 0.1 % by weight) and stir it for 30 minutes to make a homogeneous liquid mixture of bio stimulants in the form of soluble liquid formulation.

Embodiment: 5
[54] In this embodiment, a suspension liquid bio stimulant was prepared. The organic multifunctional acids such as glycinic acid (10-25% by weight), cinnamic acid (1.0 to 5.0% by weight) and lipoic acid (10 to 25% by weight) are dissolved in the required quantity of water and followed by nitrogenous compounds of tetrazine (5 to 10% by weight) and mono ethanol amide (5 to 25% by weight). Then add alkyl aryl sulfonate (2 to 6% by weight) and EG (4 to 10 % by weight). Subsequently, add carboxyl methyl cellulose (4% solution, 10 to 20% by weight) as a suspending agent, propylthio uracil (1 to 5% by weight), micronutrients of zinc sulfate mono hydrate (1 to 8% by weight) and sodium benzoate (0.01 to 0.1 % by weight) and stir it for 30 minutes using a homogenizer to make a homogeneous suspension liquid mixture of bio stimulants in the form of soluble liquid formulation.
Embodiment: 6
[55] In this embodiment, a flowable concentration suspension liquid bio stimulant was prepared. The organic multifunctional acids such as salicylic acid (10-25% by weight), poly ascorbic, acid (1.0 to 10.0% by weight) and polyglycolic acid (5 to 25% by weight) are dissolved in the required quantity of water and followed by nitrogenous compounds of guanine (5 to 10% by weight) and triazine triamine (5 to 40% by weight). Then add alkyl aryl sulfonate (2 to 6% by weight), antioxidant Mg ascorbyl phosphates (0.5 to 2.5% by weight) and glycine (4 to 10 % by weight). Subsequently, add guar gum (4% solution, 10 to 20% by weight) as a suspending agent and sodium benzoate (0.01 to 0.1 % by weight) and stir it for 30 minutes using a homogenizer to make a homogeneous flowable suspension liquid mixture of bio stimulants in the form of soluble liquid formulation.
Embodiment: 7
[56] In this embodiment, a suspension concentration liquid bio stimulant was prepared. The organic multifunctional acids such as thiosalicylic acid (10-25% by weight), polyascorbic acid (1.0 to 10.0% by weight) and polyglycolic acid (5 to 25% by weight) are dissolved in the required quantity of water and followed by nitrogenous compounds of guanine (5 to 10% by weight) and triazine triamine (5 to 40% by weight). Then addalkyl aryl sulfonate (2 to 6% by weight), antioxidant pentaerythritol-tetrakis-3-(3,5-ditert butyl-4-hydroxyphenyl) propionate (0.5 to 1.5% by weight), magnesium carbonate (0.7 to 1.9% by weight) and glycine (4 to 10 % by weight). Subsequently, add guar gum (4% solution, 10 to 20% by weight) as a suspending agent and sodium benzoate (0.01 to 0.1 % by weight) and stir it for 30 minutes using a homogenizer to make a homogeneous flowable suspension liquid mixture of bio stimulants in the form of soluble liquid formulation.
Embodiment: 8
[57] In this embodiment, a suspension concentration liquid bio stimulant was prepared. Take the required amount of water (25 to 60 gm) add alkyl aryl sulfonate (2 to 6 %) and glycerin (4 to 10 % by weight) and stir well for 10 minutes. Then add organic multifunctional acids such as thiosalicylic acid (10-45% by weight), polyascorbic acid (1.0 to 30.0% by weight) and polyglycolic acid (5 to 25% by weight) are added into the above mixture and homogenous the solution for another 15 minutes. Followed by adding the required quantity of nitrogenous compounds of guanine (5 to 10% by weight) and triazine triamine (5 to 40% by weight). Finally, add sodium benzoate (0.01 to 0.1 % by weight), antioxidant Nord hydro guaiacic acid (1 to 3% by weight), manganese sulphate mono hydrate (1 to 8% by weight) and stir it for 30 minutes using a homogenizer to make a homogeneous suspension liquid mixture. This mixture was milled in a ball mill to reduce the size of the particles to less than 3 mm. This suspension liquid acts as an effective bio stimulant.
Embodiment: 9
[58] In this embodiment, coated bio stimulant granules were prepared. The organic multifunctional acids such as histidine acid (10-25% by weight), polyglutamic acid (1.0 to 10.0% by weight) and polyglycolic acid (5 to 25% by weight) are dissolved in the minimum required quantity of water and followed by nitrogenous compounds of guanine (5 to 10% by weight) and triazine triamine (5 to 40% by weight). Then add alkyl aryl sulfonate (2 to 6% by weight), antioxidant butyl hydroxy anisol (1 to 3.5 % by weight) and elemental sulfur powder (5 to 10% by weight). The above mixture was stirred for 30 minutes to get a homogenous liquid concentration liquid. This liquid was coated to bentonite granules sized 3 to 5 mm and dried to get a final coated GR form ofbio stimulant.
Embodiment: 10
[59] In this embodiment, a soluble granules bio stimulant was prepared. The organic multifunctional acids such as glycine amino acid (10-25% by weight), fulvic acid (5.0 to 20.0% by weight) and humic acid derivatives (15 to 30% by weight) are dissolved in the required quantity of water and followed by nitrogenous compounds of polyamine (5 to 10% by weight) and monoethanol amine (5 to 20% by weight). Then add sodium lignosulphonate 2 to 6% by weight. Subsequently, add lactic acid as a soluble filler in the range of 25 to 50% by weight and blend properly. This semisolid mixture was extruded in the size range of 2 to 5 mm and dried at 50oC in the form of soluble granules (SG).
Embodiment: 11
[60] In this embodiment, a microemulsion concentration liquid bio stimulant was prepared. Take the required amount of water (25 to 60 % by weight) and sodium lignosulphonate (2 to 6 %), polyoxyethylene alkyl aryl phosphoric acid ester (2 to 5 % by weight) and propylene glycol (4 to 10 % by weight) and stir well for 15 minutes. Then organic multifunctional acids such as folic acid (2-4 % by weight), pipecolic acid (1.0 to 13.0% by weight) and gallic acid (5 to 25% by weight) are added into the above mixture and homogenous the solution for another 15 minutes. Followed by adding the required quantity of nitrogenous compounds of guanine (5 to 10% by weight) and cysteine (5 to 20% by weight). Finally, add (0.1 to 2.0 %) and sodium benzoate (0.01 to 0.1 % by weight) and stir it for 30 minutes using a homogenizer to make a homogeneous suspension liquid mixture. This mixture was milled in a ball mill to reduce the size of the particles to less than 2 mm. This microemulsion liquid formulation acts as an effective bio stimulant.
Embodiment: 12
[61] In this embodiment, a soluble granules bio stimulant was prepared. The organic multifunctional acids such as N-naphthyl acid (2.0-5.0% by weight), fulvic acid (5.0 to 20.0% by weight) and humic acid derivatives (15 to 30% by weight) are dissolved in the required quantity of water and followed by nitrogenous compounds of polyamine (5 to 10% by weight) and glycine betaine (5 to 20% by weight). Then add zinc gluconate (0.5 to 5.0 %) and sodium lignosulphonate 2 to 6% by weight. Subsequently, add lactic acid as a soluble filler in the range of 25 to 50% by weight and blend properly. This semi solid mixture was extruded in the size range of 2 to 5 mm and dried at 50oC in the form of soluble granules (SG).
Embodiment: 13
[62] In this embodiment, a suspension concentration liquid bio stimulant was prepared. Take the required amount of water (25 to 60 gm) add alkyl aryl sulfonate (2 to 6 %) and glycerin (4 to 10 % by weight) and stir well for 10 minutes. Then add organic multifunctional acids such as thiosalicylic acid (10-45% by weight), polyascorbic acid (1.0 to 30.0% by weight) and polyglycolic acid (5 to 25% by weight) are added into the above mixture and homogenous the solution for another 15 minutes. Followed by adding the required quantity of nitrogenous compounds of guanine (5 to 10% by weight) and dicyanide (5 to 40% by weight) at constant stirring. Then add calcium citrate (2.0 to 10%) and manganese carbonate (1.0 to 6.0%) to mix properly. Finally, add sodium benzoate (0.01 to 0.1 % by weight), antioxidant Nord hydro guaiacic acid (1 to 3% by weight), manganese sulphate mono hydrate (1 to 8% by weight) and stir it for 30 minutes using a homogenizer to make a homogeneous suspension liquid mixture. This mixture was milled in a ball mill to reduce the size of the particles to less than 3 mm. This suspension liquid acts as an effective bio stimulant.

Experimental results
[63] As per embodiments, the term "increased yield" of an agricultural plant denotes a large increase in the yield of a plant's product when compared to the same plant produced under identical conditions without utilizing the agricultural composition as disclosed. An embodiment states that "increased nutrient uptake" refers to enhanced plant metabolism and ion transport, both of which raise the nutritious content of fruits and vegetables. Nutritious elements including minerals, proteins, vitamins, carbs, and others are abundant in fruits and vegetables. "Improved plant vigour" pertains to the enhancement of specific traits of the plant or crop, including postponed senescence, root growth, longer panicles, improved plant stand, plant weight and height, improved visual appearance, improved vitality of the plant, improved quality of the plant, improved quality of the fruits or vegetables, the improved defense mechanism of the plant such as induced tolerance against fungi, bacteria, viruses and/or insects as well as biotic and abiotic stresses.
[64] The following Table 1 shows the various food crops, vegetable crops, flowing plantation and fruit crops were used to establish the bio efficacy of the newly prepared bio stimulants. This experiment which mentioned below as an example of the various crop field tests that are conducted across the nation to confirm the impact of the bio-stimulant application on crop growth-promoting and quality-improving effects on soil (verification results show that the bio-stimulant of the above-mentioned embodiments can all produce better growth-promoting and quality-improving effects on crops). Various crop field tests are carried out across the country to confirm the bio efficacy of the new bio stimulants of the application of both foliar, soil broadcasting, soil drenching, drone application and drip irrigation to promote the growth and quality of the crops.

Name of Crops Age of Crop after seedling (in weeks) Dosage (ml or gm/litre) Type of Application Plant Height (in cm) No. of branches (in Numbers) Vigor growth (in %) Material Used Overall Yield (in %)
Tomato 4 3 Foliar 58.5
41.2 16
11 32
21 Biostimulant through Embodiment 1.
Ideal farmer practice 34
17.6
-
Okra 6 3 Foliar 22.4
15.8 18
11 25
19 Biostimulant through Embodiment 4.
Ideal farmer practice 28.5
19.3
-
Egg plant 3 5 Soil Drenching 13.4
7 14
9 23
18 Biostimulantthrough Embodiment 6.
Ideal farmer practice 30.1
18
-
Cucumber 8 5 Soil Application 52.3
40.1 15
10 33
24 Biostimulantthrough Embodiment 2.
Ideal farmer practice 32.4
23.5
-
Chilli 5 4 Foliar 48.9
35.7 18
12 25
17 Biostimulantthrough Embodiment 7.
Ideal farmer practice 28.4
16.2
-
Paddy 4 3 Foliar 34.2
23.6 24
18 28
21 Biostimulantthrough Embodiment 9.
Ideal farmer practice 27.3
16
-
Wheat 4 5 Foliar 16.9
9.8 21
15 24
18 Biostimulantthrough Embodiment 8.
Ideal farmer practice 26.4
15.5
-
Maize 5 5 Soil Application 52
39.3 9
5 26
18 Biostimulant throughEmbodiment 11.
Ideal farmer practice 23.4
17.9
-
Rose 25 10 Soil Application 78.5
55.4 8
5 24
16 Biostimulant through Embodiment 3
Ideal farmer practice 19
13.8
-
Jasmine
102 5 Soil Drenching 128.6
103.4 7
3 26
18 Biostimulantthrough Embodiment 12.
Ideal farmer practice 21.4
18.5
-
Grapes 6 3 Foliar 98.1
80.2 14
8 18
12 Biostimulantthrough Embodiment 10.
Ideal farmer practice 27.8
20.5
-
Pomegranate 50 5 Soil Drenching 31.5
24.6 16
11 26
19 Biostimulantthrough Embodiment 13.
Ideal farmer practice 23.4
14.2
-
Apple 50 5 Foliar 178
156.4 12
8 23
20 Biostimulantthrough Embodiment 11.
Ideal farmer practice 18.6
12.3
-
Orange 360 5 Foliar 150.5
146.2 14
9 24
18 Biostimulantthrough Embodiment 10.
Ideal farmer practice 27.5
19.1
-
Watermelon 25 10 Drip Irrigation 15.3
19.2 15
10 27
20 Biostimulantthrough Embodiment 2.
Ideal farmer practice 29.3
20.5
-

TABLE 1

[65] It is to be recognized that any technical alternatives that function similarly to achieve a comparable goal are included in the list of particular terms that have been chosen, but it is not intended for the invention to be restricted to them. It is acknowledged that every numerical range mentioned here is meant to encompass all subranges that are included. Additionally, the weight % of each component in a composition is shown unless otherwise indicated.
[66] Although the foregoing subject matter has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be understood by those skilled in the art that certain changes and modifications can be practiced within the scope of the appended claims.

, Claims:
We claim:

1. A bio stimulant, comprising:
a bioactive nitrogen-containing organic compound;
a multi-functional organic acid;
a dispersing agent; and
an anticaking agent
wherein the ratio of nitrogenous organic chemical compounds to multifunctional organic acid is 800:1 to 1:750.

2. The bio stimulant as claimed in claim 1, wherein the nitrogen containing organic compound is chosen from: heterocyclic nitrogenous compounds (i.e., triazine, triazole, diazine, tetrazine, tetrazole), cynamides (i.e., dicyanamide), amines (i.e., aromatic amine, aromatic heterocyclic amines), polyamines, imines, amino acids (Essential and Non-essential amino acids), alcohols, polymeric nitrogenous compounds, adenine and guanine.

3. The bio stimulant as claimed in claim 1, wherein organic bulkier multifunctional acid is chosen from : humic, fulvic, folic, ascorbic, gluconic, polyglycolic, citric, tartaric, oxalic, lactic, polylactic, thiazolic, succinic, 9-oxononanoic, arachidonic, pipecolic, salicylic, phosphonic, gallic, maleic, polymaleic, adipic, tannic, alginic, glycinic, barbituric, thiosalicylic, ascorbic acid, polyascorbic, polytartaric, cinnamic, jasmonic,Lipoic acid (LA), glutathione (GSH), betainglycine.

4. The bio stimulant as claimed in claim 1, wherein the surfactants are either cationic, anionic or non-ionic surfactant.

5. The bio stimulant as claimed in claim 1, wherein the bio stimulants are formulated assoluble powder (SP), soluble liquid (SL), soluble granules (SG), granules (GR), gel (GEL), flowable concentration (FS), suspension concentration (SC), wettable powder (WP), water dispersible granules (WDG), microemulsion (MC) and nano colloidal (NC) forms.

6. The bio stimulant as claimed in claim 5, wherein the nitrogen containing organic compound in liquid form comprises: one or more amino acids selected from alanine, arginine, aspartic acid, asparagine, citrulline, leucine, lysine, isoleucine, cysteine, glutamic acid, glutamine, glycine, gaba-aminobutyric acid, histidine, methionine, ornithine, proline, phenylalanine, serine, selenocysteine, valine, taurine, tyrosine, theanine, threonine, tryptophan, peptides, plant and animal protein, polyglutamic acid, polyglutamic acid, protein hydrolysate, soya protein hydrolysate, whey protein hydrolysate, fish protein hydrolysate.

7. The bio stimulantas claimed in claim 1 further comprises at least one micronutrient, their salts, derivatives or mixtures thereof present in the concentration of 0.1%-40% by weight of the total composition; wherein the micronutrient is selected from zinc, iron, copper, boron, manganese, silicon, cobalt, selenium, molybdenum, their salts, derivatives or mixtures thereof.

8. The bio stimulant as claimed in claim 1 further comprises at least one secondary nutrients, their salts, derivatives or mixtures thereof present in the concentration of 0.1%-30% by weight of the total composition; wherein the micronutrient is selected from calcium, magnesium and sulphur, their salts, derivatives or mixtures thereof.

9. The bio stimulant as claimed in claim 1, further comprises at least one plant growth promoter or fertilizer or pesticidal active, wherein the plant growth promoter is selected from triaconatol, ascorbic acids, lactic acid, oxalic acid, citric acid, N-acetyl thiazolidine-4 carboxylic acid, paclobutrazol, phytic acid, fumaric acid, gibberelic acid, auxins or mixtures thereof present in the concentration of 0.1-30% w/w of the total composition.

10. The biostimulant as claimed in claim 1, wherein one ormore agrochemical acceptable excipients are selected from one or more surfactants, fillers or carriers or diluents, spreading agents, colourants, binders, buffers or pH adjusters or neutralizing agents, antifoaming agents or defoamers, humectants, preservatives, UV ray scattering agents, anti-freezing agents, stabilizers, sticking agents and spreading agents, anti-settling agents, penetrants, hydrophobic agents, ultraviolet absorbentsand mixtures thereof.

11. A method of formulating a bio stimulant, the method comprising

milling and blending of an organic nitrogenous chemical compound, at least one organic multifunctional acid, their salts, derivatives and at least one agrochemical acceptable excipient to obtain a slurry or wet mix as claimed in claims 1-10;
drying the wet mix to obtain the water-dispersible granular composition; wherein the granules of the composition comprise particles in the size range of 0.1 microns to 20 microns.