FORM 2
THE PATENT ACT 1970
& The Patents Rules, 2003
COMPLETE SPECIFICATION (See section 10 and rule 13)
1. TITLE OF THE INVENTION:
"Cleaning Composition for Farm Produce"
2. APPLICANT
(a) NAME: Autodynamic Engineering Private Limited
(b) NATIONALITY: Indian Company Registered Under the Companies
Act 1956
(c) ADDRESS: H - 402, Pinnae Kanchanganga, D. P. Road, Aundh
Pune-411007 Maharashtra, India
3. PRREAMBLE TO THE DESCRIPTION
COMPLETE
The following specification particularly describes the invention and the manner in which it is to be performed.

CLEANING COMPOSITION FOR FARM PRODUCE
Field of invention
The present invention relates to cleaning compositions, and more particularly, to a cleaner adapted for cleaning of farm produce, such as for example vegetables and fruits.
Background of the invention
Farm produce such as fruits and vegetables are highly nutritious and form a key food commodity in the human consumption. However, the farm produce are highly perishable due to their low shelf life. These food commodities are reported to be contaminated with toxic and health hazardous chemicals. Prior to consumption, it is important that the fruits and vegetables should be thoroughly washed to remove harmful bacteria, chemicals, soils and other unwanted residues.
Further, the fruits are coated with waxy coatings for increasing their shelf life, retaining their moisture and improving their appearance. Such waxy coatings need to be essentially removed before consumption. Currently, majority of the consumers use tap water for washing the vegetables and fruits. However, the use of the tap water for cleaning the vegetables and fruits removes only the dirt but not the harmful bacteria and chemicals.
The use of detergents has substantial limitations in cleaning the farm produce. The prior art detergents in the art normally use harsh chemicals that are likely to damage the quality of the farm produce. Further, the currently available detergents are not designed for specific use of washing of fruits and vegetables.
Accordingly, there exist a need to provide a detergent composition which overcomes all the drawbacks of the prior art.
Object of the invention

An object of the present invention is to provide a cleaning composition specifically adapted for washing of farm produce and a process for preparation thereof.
Another object of the present invention is to provide an environmentally compatible cleaning composition which removes harmful bacteria, mud, dirt, wax coatings and harmful chemicals from the surface of the farm produce.
Summary of the invention
Accordingly, the present invention provides an environmentally compatible cleaning composition adapted for washing farm produce, the cleaning composition comprising:
a) from about 1% by weight to about 2% by weight of a salt of citric acid;
b) from about 1% by weight to about 2% by weight of a salt of carbonic acid,;
c) from about 2% by weight to about 5% by weight of a salt of oleic acid,;
d) from about 0.5% by weight to about 1.5% by weight of a non-ionic surfactant;
e) from about 0.1% by weight to about 1.0% by weight of a salt of benzoic acid;
f) from about 1% by weight to about 3% by weight of a solvent;
g) from about 0.5% by weight to about 1.5% by weight of a natural preservative; h) from about 0.5% by weight to about 1.5% by weight of a citrus extract;
i) from about 0.1% by weight to about 0.5% by weight of an antibacterial and
antifungal agent; j) from about 0.1%o by weight to about 1.0% by weight of a natural food perfume;
and k) Balance quantity of an aqueous carrier.
Detail description of the invention
The foregoing objects of the present invention are accomplished and the problems and shortcomings associated with the prior art, techniques and approaches are overcome by the present invention as described below in the preferred embodiment.

The present invention provides a cleaning composition for washing of farm produce. Further, the cleaning composition provides an environmentally compatible cleaning composition which removes harmful bacteria, mud, dirt, wax coatings and harmful chemicals from the surface of the farm produce.
In the context of the present invention, the cleaning composition comprising:
a) from about 1% by weight to about 2% by weight of a salt of citric acid, optionally selected from sodium citrate or potassium citrate;
b) from about 1% by weight to about 2% by weight of a salt of carbonic acid, optionally selected from sodium carbonate or potassium carbonate;
c) from about 2% by weight to about 4% by weight of a salt of oleic acid, optionally selected from sodium oleate or potassium oleate;
d) from about 0.5% by weight to about 1.5% by weight of a non-ionic surfactant in general and fatty alcohol ethoxylate in particular;
e) from about 0.1%) by weight to about 1.0% by weight of a salt of benzoic acid optionally selected from sodium benzoate or potassium benzoate;
f) from about 1% by weight to about 3% by weight of a solvent in general and ethanol in particular;
g) from about 0.5% by weight to about 1.5% by weight of a natural preservative/conservative in general and citric acid in particular;
h) from about 0.5% by weight to about 1.5% by weight of a citrus extract, optionally
selected from Neem extract or lemon extract or vinegar extract; i) from about 0.1% by weight to about 0.3% by weight of an antibacterial and
antifungal agent in general and Triclosan in particular; j) from about 0.1% by weight to about 0.3% by weight of a natural food perfume;
and k) balance quantity of an aqueous carrier in general and demineralized water in
particular.

In the context of the present invention, a method or process for preparation of the above mentioned cleaning composition is described hereinafter, wherein the said process is comprising the steps of:
a) Optionally preparing a predefined amount of sodium citrate or potassium citrate solution;
b) Optionally preparing and adding a predefined quantity of sodium carbonate or potassium' carbonate solution;
c) Optionally preparing and adding a predefined quantity of sodium oleate or potassium oleate;
d) Adding a predefined quantity of fatty alcohol ethoxylate;
e) Optionally preparing and adding a predefined quantity of sodium benzoate or potassium benzoate solution;
f) Adding a predefined quantity of ethanol;
g) Adding a predefined quantity of citric acid;
h) Optionally adding a predefined quantity of Neem extract or lemon extract or
vinegar extract; i) Adding a predefined quantity of Triclosan; j) Adding a predefined quantity of natural food perfume; and k) Adding balance quantity of demineralized water 1) Mixing all the constituents in above mentioned steps from a) through k) to get the
cleaning composition of the present invention.
In the context of the preferred embodiment, the sodium or potassium oleate used in step c) of the above mentioned process can optionally prepared by a method or process comprising the steps of:
a) Heating a predefined quantity of oleic acid to a predefined temperature;
b) Optionally mixing a predefined quantity of sodium hydroxide or potassium hydroxide with a predefined quantity of water to form a hydroxide solution;
c) Mixing the predefined quantity of hydroxide solution with the predefined quantity of heated oleic acid to form a predefined quantity of sodium or potassium oleate; and

d) Optionally mixing the predefined quantity of sodium or potassium oleate solution with a predefined quantity of purified water thereby forming a homogeneous mixture of sodium or potassium oleate.
In an alternative embodiment of the present invention, the cleaning composition of the present invention comprising:
a) from about 1% by weight to about 3% by weight of oleic acid;
b) from about 1% by weight to about 3% by weight of glycerol;
c) from about 1% by weight to about 2% by weight of a base, optionally selected from sodium hydroxide or potassium hydroxide;
d) from about 1% by weight to about 3% by weight of a solvent in general and ethanol in particular;
e) from about 0.5% by weight to about 1.5% by weight of a poly ethylene glycol in general and PEG 8000 in particular;
f) from about 0.5% by weight to about 1.0% by weight of a salt of carbonic acid optionally selected from sodium carbonate or potassium carbonate;
g) from about 0.5% by weight to about 1.0% by weight of a natural preservative/conservative in general and citric acid in particular;
h) from about 0.5% by weight to about 1.0% by weight of a citrus extract, optionally
selected from neem extract or lemon extract or vinegar extract; i) from about 0.1% by weight to about 0.3% by weight of an antibacterial and
antifungal agent in general and triclosan in particular; j) from about 0.1% by weight to about 0.3% by weight of a natural food perfume;
and k) balance quantity of an aqueous carrier in general and demineralized water in
particular.
In this one alternative embodiment, a method or process for preparation of the cleaning composition is described hereinafter, wherein the process comprising the steps of: e) Measuring a predefined quantity oleic acid;

f) Optionally preparing and adding a predefined quantity of sodium or potassium
hydroxide;
g) Adding of a predefined quantity of glycerol;
h) Adding of a predefined quantity of PEG 8000;
i) Optionally preparing and adding a predefined quantity of sodium or potassium
carbonate solution; j) Adding a predefined quantity of ethanol; k) Adding a predefined quantity of citric acid; 1) Optionally adding a predefined quantity of neem extract or lemon extract or
vinegar extract; m) Adding a predefined quantity of triclosan; n) Adding a predefined quantity of natural food grade perfume; o) Adding a balanced quantity of demineralized water to form a homogeneous
mixture; and p) Stirring of the homogeneous mixture to obtain the cleaning composition of the
present invention.
Accordingly, in the context of the present invention, the tests that are conducted to ascertain the cleaning activity of the cleaning composition are described hereinafter:
a) In an active ingredient residue analysis study, a predefined amount of the cleaning composition of the present invention is applied to a sample of farm produce such as a fruit or a vegetable which is followed by repetitive washing of water. The farm produce sample is then tested by gas chromatography, most preferably by GCMS, for active ingredient residues.
b) In a bacteria test study, a predefined amount of the farm produce sample, such as a fruit or a vegetable, is spiked with microorganisms and tested for bacteria like E.coli, S.aureus, Salmonella spp., and Listeria monocytogenes. The farm produce sample is then washed with a predefined amount of the cleaning composition. The farm produce sample after washing with the cleaning composition is tested for bacteria like E.coli, S.aureus, Salmonella spp., and Listeria monocytogenes.

c) In a Chloropyrifos test study, a predefined amount of farm produce sample, such as a fruit or a vegetable, is spiked with Chlorpyrifos. The farm produce sample is then washed with a predefined amount of the cleaning composition of the present invention. The farm produce sample, after washing with the cleaning composition, is tested again for Chlorpyrifos.
d) In a dirt and mud removal test study, a predefined amount of farm produce sample, such as a fruit or a vegetable, is spiked with dirt and mud. The farm produce sample is then washed with a predefined amount of the cleaning composition of the present invention. The farm produce sample, after washing with the cleaning composition, is tested again for dirt and mud.
Examples:
Example 1:
Preparation of sodium based cleaning composition without preparation of sodium oleate-A solution of sodium citrate containing 10 gm/1000 ml of sodium citrate was prepared. A solution containing lOgm/1000 ml of sodium carbonate was added followed by adding of 20 ml/1000ml of sodium oleate. 5 ml/1000ml of alcohol ethoxylate was added. A solution containing Igm/ 1000ml of sodium benzoate was prepared. 10 ml/ 1000ml of ethanol was added. 5ml/l 000ml of citric acid was added. 5 ml/1000ml of neem extract was added. 1 ml/1000ml of Triclosan was added followed by adding of 1 ml/1000ml of natural food perfume. The balance quantity of demineralized water was added followed by stirring to get 1000 ml of cleaning composition of the present invention. The cleaning composition so obtained was micro-filtered and packed per the requirement.
Example 2:
Preparation of sodium based cleaning composition with preparation of sodium oleate-100 gms. of oleic acid was gently heated to 80 °C. 5 gms of sodium hydroxide was mixed in 35 gms of purified water to get a sodium hydroxide solution. The sodium hydroxide

solution was added to the heated oleic acid to get sodium oleate solution. The sodium oleate solution was added with 900 ml of purified water till a homogenous mixture was formed. The 20 ml homogeneous mixture was added with 10 ml of ethanol. Subsequently, 10gm/l 000ml of sodium citrate, 10 gm/1000ml of sodium carbonate, 5ml/1000ml of fatty alcohol ethoxylate, lml/lOOOml of sodium benzoate, 5 ml/1000ml of citric acid, 5ml/1000ml of neem extract, lml/lOOOml of Triclosan, 1 ml/lOOOml of natural food perfume were added. The balance quantity of demineralized water was added followed by stirring to get 1000 ml of the cleaning composition. The cleaning composition so obtained was micro-filtered and packed per the requirement.
Example 3:
Preparation of potassium based cleaning composition without preparation of potassium oleate-
A solution of potassium citrate containing 10 gm/1000ml of potassium citrate was prepared. A solution containing 10 gm/1000ml of potassium carbonate was added followed by adding of 20 ml/1000ml of potassium oleate. 5ml/1000ml of alcohol ethoxylate was added. A solution containing 1 gm/1000ml of potassium benzoate was prepared. 10ml/1000ml of ethanol was added. 5ml/1000mi of citric acid was added. 5 gm/1000ml of neem extract was added. 1 ml/1000ml of of Triclosan was added followed by adding of lml/lOOOml of natural food perfume. The balance quantity of demineralized water was added followed by stirring to get 1000ml of cleaning composition of the present invention. The cleaning composition so obtained was micro-filtered and packed per the requirement.
Example 4:
Preparation of potassium based cleaning composition with preparation of potassium
oleate-
100 gms. of oleic acid was gently heated to 80 °C. 5 gms of potassium hydroxide was
mixed in 35 gms of purified water to get a potassium hydroxide solution. The potassium
hydroxide solution was added to the heated oleic acid to get potassium oleate solution.
The potassium oleate solution was added with 900 ml of purified water till a homogenous

mixture was formed. The 20 ml homogeneous mixture was added with 10 ml of ethanol. Subsequently, 10gm/1000ml of potassium citrate, 10 gm/1000ml of potassium carbonate, 5ml/1000ml of fatty alcohol ethoxylate, lml/lOOOml of potassium benzoate, 5 ml/1000ml of citric acid, 5ml/l 000ml of neem extract, lml/lOOOml of Triclosan, 1 ml/lOOOml of natural food perfume were added. The balance quantity of demineralized water was added followed by stirring to get 1000 ml of the cleaning composition. The cleaning composition so obtained was micro-filtered and packed per the requirement.
Example 5:
Active ingredient residue analysis study-Vegetable sample was treated with 3% of the cleaning composition for a period of 1 minute followed by repetitive washing with the pure water. The residue of active ingredients of the cleaning composition on the vegetable sample was analyzed on GCMS along with a blank sample. It was observed that active ingredient residue result was NIL in the view of detection limit 0.1 mg/kg. Accordingly, safety of the cleaning composition for its intended human use was ascertained.
Example 6: Bacteria test study-Tomato sample admeasuring about 1 kg was spiked with microorganisms and tested before and after application of the cleaning composition of the present invention. The cleaning composition was also tested after its treatment with the tomato sample spiked with the microorganisms. The test methods used were IS: 5887 (Part-I):1976 (Reaff.2005), IS: 5887 (Part-Ill): 1999 (Reaff.2005) and ISO: 11290 (Part-II): 1998. The test parameters selected were E.coli, S.aureus, Salmonella Spp., Listeria Monocytogenes. It was observed that bacteria namely E.coli, S.aureus, Salmonella Spp., Listeria Monocytogenes were substantially reduced after treatment with the cleaning composition.
Table-1: Bacterial analysis of Tomatoes spiked with Microorganisms

Test Parameters Method Result Limit of reporting Unit

(LOR)
E.coli IS:5887(Part-I):1976 (Reaff.2005) 8.0 x 10' 10.00000 cfu/g
S. Aureus IS:5887(Part-II):1976
(Reaff.2005) 5.2x10' 10.00000 cfu/g
Salmonella Spp. IS:5887(Part-III):1999 (Reaff.2005) 4.0 x 10' 10.00000 cfu/g
Listeria ISO: 11290 (Part-II): 1998 4.4 x 10' 10.00000 cfu/g
Monocytogenes
Table-2: Tomatoes spiked with Microorganisms and washed with the cleaning composition

Test Parameters Method Result Limit of
reporting
(LOR) Unit
E.coli IS:5887(Part-I):1976 (Reaff.2005) <10 10.00000 cfu/g
S. Aureus IS:5887(Part-II):1976 (Reaff.2005) 210 10.00000 cfu/g
Salmonella Spp. IS:5887(Part-III):1999 (Reaff.2005) 150 10.00000 cfu/g
Listeria Monocytogenes ISO: 11290 (Part-II): 1998 <10 10.00000 cfu/g
Table-3: Bacterial Analysis of the cleaning composition after treatment on Tomatoes spiked with Microorganisms

Test Parameters Method Result Limit of
reporting
(LOR) Unit
E.coli IS:5887(Part-I):1976 <1 1.00000 cfu/ml

(Reaff.2005)
S. aureus IS:5887 (Part-II): 1976
(Reaff.2005) 50 1.00000 cfu/ml
Salmonella Spp. IS:5887 (Part-Ill): 1999 (Reaff.2005) 100 1.00000 cfu/ml
Listeria ISO: 11290 (Part-II): 1998 <1 1.00000 cfu/ml
Monocytogenes
Example 7:
Chloropyrophos test study-Tomato sample admeasuring about 1 kg was spiked with chloropyrophos and tested before and after application of the cleaning composition of the present invention. The test method used was AOAC 970.52. The test parameters selected were chloropyrophos. It was observed that chloropyrophos was substantially reduced after treatment with the cleaning composition.
Table-4: Chloropyrophos Analysis of Tomatoes spiked with Microorganisms

Test Parameters Method Result Detection Limit Unit
Chloropyrophos AOAC 970.52 16.35 0.01 mg/kg
Table-5: Chloropyrophos Analysis of Tomatoes spiked with Microorganisms

Test Parameters Method Result Detection Limit Unit
Chloropyrophos AOAC 970.52 <0.01 0.01 mg/kg
Example 8:
Dirt and mud removal test study-Tomato sample admeasuring about 1 kg was spiked with dirt and/or mud. The cleaning composition was tested before and after application of the cleaning composition of the present invention. The testing was done physcially. The test parameters selected was presence of dirt in the sample. It was observed that the dirt was present before treatment

with the cleaning composition. It was observed that the dirt was absent after treatment with the cleaning composition.
Advantages of the invention
1. The cleaning composition of the present invention removes chemicals like
pesticides, preservatives, artificial ripeners, soil, dirt and wax from the fruits and
vegetables than washing with water alone.
2. The cleaning composition of the present invention kills harmful bacteria such as E. Coli, Salmonella Species., Staphylococcus aureus, and Listeria which are associated with food-borne illness.
3. The cleaning composition of the present invention completely washes away with no residual taste, leaving the natural taste of fruits and vegetables.
4. The cleaning composition of the present invention is 100% natural and unlike chlorine
or other harsh chemicals, and completely safe to human use.
5. The cleaning composition of the present invention is in liquid form that provides ease for cleaning in the farm produce, such as fruits and vegetables.
6. The cleaning composition of the present invention is usable in various segments including but not limited to households, commercial establishments, farms, food processing industries and packaging industries.
The foregoing descriptions of specific embodiments of the present invention have been presented for purposes of illustration and description. They are not intended to be exhaustive or to limit the present invention to the precise forms disclosed, and obviously many modifications and variations are possible in light of the above teaching. The

embodiments were chosen and described in order to best explain the principles of the present invention and its practical application, to thereby enable others skilled in the art to best utilize the present invention and various embodiments with various modifications as are suited to the particular use contemplated. It is understood that various omission and substitutions of equivalents are contemplated as circumstance may suggest or render expedient, but such are intended to cover the application or implementation without departing from the spirit or scope of the present invention.

We claim:
1. An environmentally compatible cleaning composition adapted for washing farm
produce, the cleaning composition comprising:
a) from about 1% by weight to about 2% by weight of a salt of citric acid;
b) from about 1% by weight to about 2% by weight .of a salt of carbonic acid,;
c) from about 2% by weight to about 4% by weight of a salt of oleic acid,;
d) from about 0.5% by weight to about 1.5% by weight of a non-ionic surfactant;
e) from about 0.1% by weight to about 1.0% by weight of a salt of benzoic acid;
f) from about 1% by weight to about 3% by weight of a solvent;
g) from about 0.5% by weight to about 1.5% by weight of a natural preservative;
h) from about 0.5% by weight to about 1.5% by weight of a citrus extract;
i) from about 0.1% by weight to about 0.3% by weight of an antibacterial and
antifungal agent; j) from about 0.1% by weight to about 0.3% by weight of a natural food perfume;
and k) balance quantity of an aqueous carrier.
2. The cleaning composition as claimed in claim 1, wherein the salt of citric acid is
selected from the group consisting of sodium citrate and potassium citrate.
3. The cleaning composition as claimed in claim 1, wherein the salt of carbonic acid is
selected from the group consisting of sodium carbonate and potassium carbonate.
4. The cleaning composition as claimed in claim 1, wherein the salt of oleic acid is selected from the group consisting of sodium oleate and potassium oleate.
5. The cleaning composition as claimed in claim 1, wherein the non-ionic surfactant is fatty alcohol ethoxylate.
6. The cleaning composition as claimed in claim 1, wherein the salt of benzoic acid is selected from the group consisting of sodium benzoate and potassium benzoate.

7. The cleaning composition as claimed in claim 1, wherein the solvent is ethanol.
8. The cleaning composition as claimed in claim 1, wherein the natural preservative is citric acid.
9. The cleaning composition as claimed in claim 1, wherein the citrus extract is selected from the group consisting of neem extract, lemon extract and vinegar extract.
10. The cleaning composition as claimed in claim 1, wherein the antibacterial and antifungal agent is Triclosan.