FIELD OF THE INVENTION
[001] The present disclosure relates to a composition for metabolic health. In particular, the present disclosure is related to a composition comprising jamun seed extract and grape seed extract for the management of metabolic diseases. 5
BACKGROUND OF THE INVENTION
[002] Metabolic syndrome is defined as a cluster of metabolic risk factors. It includes abdominal obesity (waist circumference of 40 inches or above in men and 35 inches or above in women), and a triglyceride level of 150 mg/dl or greater. Diabetes
10 is recognized as a global burden by the International Diabetes Federation and is often
characterized by a group of heterogeneous disorders with the common elements of hyperglycemia and glucose intolerance, caused by insulin deficiency and impaired effectiveness of insulin action, or both. [003] To address these problems, the International Diabetes Federation has
15 developed newer targets for example, alpha glucosidase inhibitors, which decrease
the rate of carbohydrate absorption from the gut, GLP-1 (glucagon-like peptide) mimetics and DPP-4 (dipeptidyl peptidase 4) inhibitors (Global guidelines for Type 2 Diabetes, Brussels: International Diabetes Federation; 2005 and 2012). [004] Dietary carbohydrate level is an important factor involved in regulating the
20 levels of carbohydrates digestion and available glucose absorption. A disturbance at
this level leads to an imbalance in fat mass and energy homeostasis, which results in metabolic disorder (Hanhineva et al., Int. J. Mol. Sci. 2010, 11, 1365-1402). Intestinal glucosidases are involved in the final step of carbohydrate digestion, which results in the conversion of complex carbohydrates to monosaccharides. Following this, these
25 carbohydrates are then absorbed through the intestine. This makes intestinal
glucosidases an important target for management of carbohydrates and one of the major glucosidases involved is the alpha-glucosidase enzyme. Thus, inhibitors of this
2

enzyme can reduce the uptake of dietary carbohydrates and suppress postprandial
hyperglycemia (Hashimoto Y et al, Bioorg. Med. Chem. Lett., 2000, 10, 1081).
[005] US 9205120 B2 relates to use of pomegranate extracts for treating diabetes
related atherosclerotic complications in humans. The methods comprise the step of
5 administering to the patients a composition comprising a therapeutically effective
amount of an extract from pomegranate.
[006] Although crucial regulators of carbohydrate metabolism have been identified,
the multifactorial nature of these diseases has made it difficult to identify potential
compounds that could help maintain homeostasis in carbohydrate metabolism. There
10 is therefore a need to develop regulators of carbohydrate levels in the body for the
management of metabolic diseases.
SUMMARY OF THE INVENTION
[007] The subject matter described herein is directed towards a synergistic
15 composition comprising fruit extracts for reduction of dietary carbohydrate
absorption.
[008] In an aspect of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; and (b) grape seed extract, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is in the range of
20 1:2 to 1:35.
[009] In an aspect of the present disclosure, there is provided a method for preparation of a composition comprising: (a) jamun seed extract; and (b) grape seed extract, said method comprising: (i) obtaining jamun seed extract; (ii) obtaining grape seed extract; and (iii) contacting jamun seed extract and grape seed extract to obtain a
25 composition, wherein the jamun seed extract to the grape seed extract w/w ratio in
said composition is in the range of 1:2 to 1:35.
[010] In an aspect of the present disclosure, there is provided a method for preparation of a composition comprising: (a) jamun seed extract; (b) grape seed
3

extract; and (c) resistant maltodextrin, said method comprising the steps of: (i)
obtaining jamun seed extract; (ii) obtaining grape seed extract; (iii) obtaining resistant
maltodextrin; and (iv) contacting jamun seed extract, grape seed extract, and resistant
maltodextrin to obtain said composition, wherein the jamun seed extract to the grape
5 seed extract w/w ratio in said composition is in the range of 1:2 to 1:35.
[011] These and other features, aspects, and advantages of the present subject matter
will be better understood with reference to the following description and appended
claims. This summary is provided to introduce a selection of concepts in a simplified
form. This summary is not intended to identify key features or essential features of
10 the claimed subject matter, nor is it intended to be used to limit the scope of the
claimed subject matter.
BRIEF DESCRIPTION OF THE DRAWINGS
[012] The following drawings form part of the present specification and are
15 included to further illustrate aspects of the present disclosure. The disclosure may be
better understood by reference to the drawings in combination with the detailed
description of the specific embodiments presented herein.
[013] Figure 1 depicts the effect of varying concentrations of jamun seed extract on
alpha glucosidase inhibition, in accordance with an embodiment of the present
20 disclosure.
[014] Figure 2 depicts the effect of varying concentrations of grape seed extract on
alpha glucosidase inhibition, in accordance with an embodiment of the present
disclosure.
[015] Figure 3 depicts the effect of a combination of jamun seed extract and grape
25 seed extract on alpha glucosidase inhibition, in accordance with an embodiment of
the present disclosure.
[016] Figure 4 depicts the effect of different bulking agents on alpha glucosidase
inhibition, in accordance with an embodiment of the present disclosure
4

[017] Figure 5 depicts the effect of a combination of jamun seed extract and grape seed extract in the presence of 10 mg/ml of resistant maltodextrin on alpha glucosidase inhibition, in accordance with an embodiment of the present disclosure.
5 DETAILED DESCRIPTION OF THE INVENTION
[018] Those skilled in the art will be aware that the present disclosure is subject to variations and modifications other than those specifically described. It is to be understood that the present disclosure includes all such variations and modifications. The disclosure also includes all such steps, features, compositions and compounds
10 referred to or indicated in this specification, individually or collectively, and any and
all combinations of any or more of such steps or features. Definitions
[019] For convenience, before further description of the present disclosure, certain terms employed in the specification, and examples are collected here. These
15 definitions should be read in the light of the remainder of the disclosure and
understood as by a person of skill in the art. The terms used herein have the meanings recognized and known to those of skill in the art, however, for convenience and completeness, particular terms and their meanings are set forth below. [020] The articles “a”, “an” and “the” are used to refer to one or to more than one
20 (i.e., to at least one) of the grammatical object of the article.
[021] The terms “comprise” and “comprising” are used in the inclusive, open sense, meaning that additional elements may be included. It is not intended to be construed as “consists of only”. [022] Throughout this specification, unless the context requires otherwise the word
25 “comprise”, and variations such as “comprises” and “comprising”, will be understood
to imply the inclusion of a stated element or step or group of element or steps but not the exclusion of any other element or step or group of element or steps.
5

[023] The term “including” is used to mean “including but not limited to”. “Including” and “including but not limited to” are used interchangeably.
[024] Unless defined otherwise, all technical and scientific terms used herein have
the same meaning as commonly understood by one of ordinary skill in the art to
5 which this disclosure belongs. Although any methods and materials similar or
equivalent to those described herein can be used in the practice or testing of the disclosure, the preferred methods, and materials are now described. All publications mentioned herein are incorporated herein by reference. [025] A composition comprising “synergistic activity” or a “synergistic
10 composition” is a combination of compounds which exhibits increased biological or
functional activity as a non-linear multiple of the biological or functional activity of the individual compounds. In other words, the combined biological or functional activity of two or more compounds being tested is significantly greater than the expected result based on independent effects of the compounds when tested
15 separately. Synergy may be apparent only at some ranges or concentrations.
[026] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the
20 disclosure, the preferred methods, and materials are now described. All publications
mentioned herein are incorporated herein by reference.
[027] The present disclosure is not to be limited in scope by the specific embodiments described herein, which are intended for the purposes of exemplification only. Functionally-equivalent products, compositions, and methods
25 are clearly within the scope of the disclosure, as described herein.
[028] The term ‘carriers’ refers to substances that serve as agents to improve the delivery and the effectiveness of drugs.
6

[029] The term diluent (also referred to as filler, diluent or thinner) refers to a diluting agent.
[030] The term ‘excipients’ refers to an inactive substance that serves as the vehicle
or medium for a drug or other active substance. Excipients include colouring agents,
5 preservatives, and fillers.
[031] Grape seed has been traditionally used in herbal medicines and is known for its antioxidant properties. It is a rich source of proanthocyanidins. It also has hepatoprotective, anticarcinogenic as well as antimicrobial effects due to the presence of oligomeric proanthocyanidins.
10 [032] Jamun has also been known for various health benefits. It is known for its
antidiabetic activity due to presence of different phytochemicals. The phytochemicals are responsible for lowering the plasma glucose levels. Its seed has been known to have antidiabetic activity. [033] Metabolic disorder is one of the major issues of the present world. Among
15 different metabolic disorders, obesity and diabetes are most prevalent and yet definite
cures to these diseases are not available. The present disclosure provides a solution to the metabolic disorders caused by the intake dietary carbohydrates by providing unique composition comprising jamun seed extract and grape seed extract which helps to significantly decrease alpha glucosidase activity, thereby regulating
20 carbohydrate availability in the body. Thus, the current composition can be used in
different formulations to address metabolic disorders.
[034] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; and (b) grape seed extract, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is in the range of
25 1:2 to 1:35.
[035] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; and (b) grape seed extract, wherein the jamun
7

seed extract to the grape seed extract w/w ratio in said composition is in the range of 1:8.3 to 1:33.3.
[036] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; and (b) grape seed extract, wherein the jamun
5 seed extract to the grape seed extract w/w ratio in said composition is 1:2.08.
[037] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; and (b) grape seed extract, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:5.5. [038] In an embodiment of the present disclosure, there is provided a composition
10 comprising: (a) jamun seed extract; and (b) grape seed extract, wherein the jamun
seed extract to the grape seed extract w/w ratio in said composition is 1:8.3. [039] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; and (b) grape seed extract, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:12.5.
15 [040] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; and (b) grape seed extract, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:33.3. [041] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) at least one bulking
20 agent, wherein the jamun seed extract to the grape seed extract w/w ratio in said
composition is in the range of 1:2 to 1:35.
[042] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) at least one bulking agent, wherein said bulking agent is selected from a group consisting of resistant
25 maltodextrin, pectin, gelatin, gums and combinations thereof, and the jamun seed
extract to the grape seed extract w/w ratio in said composition is 1:2 to 1:35. [043] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) at least one bulking
8

agent, wherein said bulking agent is resistant maltodextrin, and the jamun seed
extract to the grape seed extract w/w ratio in said composition is 1:2 to 1:35.
[044] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; (b) grape seed extract; and (c) at least one bulking
5 agent, wherein said bulking agent is gum arabic, and the jamun seed extract to the
grape seed extract w/w ratio in said composition is 1:2 to 1:35.
[045] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) at least one bulking agent, wherein said bulking agent is guar gum, and the jamun seed extract to the
10 grape seed extract w/w ratio in said composition is 1:2 to 1:35.
[046] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) at least one bulking agent, wherein said bulking agent is pectin, and the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:2 to 1:35.
15 [047] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is in the range of 1:2 - 1:35. [048] In an embodiment of the present disclosure, there is provided a composition
20 comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant
maltodextrin, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:2.08.
[049] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant
25 maltodextrin, wherein the jamun seed extract to the grape seed extract w/w ratio in
said composition is 1:5.5.
[050] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant
9

maltodextrin, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:8.3.
[051] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant
5 maltodextrin, wherein the jamun seed extract to the grape seed extract w/w ratio in
said composition is 1:12.5.
[052] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to the grape seed extract w/w ratio in
10 said composition is 1:33.3.
[053] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) gum arabic, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is in the range of 1:2 to 1:35.
15 [054] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; (b) grape seed extract; and (c) gum arabic, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:2.08. [055] In an embodiment of the present disclosure, there is provided a composition
20 comprising: (a) jamun seed extract; (b) grape seed extract; and (c) gum arabic,
wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:5.5.
[056] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) gum arabic,
25 wherein the jamun seed extract to the grape seed extract w/w ratio in said
composition is 1:8.3.
[057] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) gum arabic,
10

wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:12.5.
[058] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; (b) grape seed extract; and (c) gum arabic,
5 wherein the jamun seed extract to the grape seed extract w/w ratio in said
composition is 1:33.3.
[059] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) guar gum, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is in
10 the range of 1:2 to 1:35.
[060] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) guar gum, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:2.08.
15 [061] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; (b) grape seed extract; and (c) guar gum, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:5.5. [062] In an embodiment of the present disclosure, there is provided a composition
20 comprising: (a) jamun seed extract; (b) grape seed extract; and (c) guar gum, wherein
the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:8.3.
[063] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) guar gum, wherein
25 the jamun seed extract to the grape seed extract w/w ratio in said composition is
1:12.5.
[064] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) guar gum, wherein
11

the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:33.3.
[065] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; (b) grape seed extract; and (c) pectin, wherein the
5 jamun seed extract to the grape seed extract w/w ratio in said composition is in the
range of 1:2 to 1:35.
[066] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) pectin, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:2.08.
10 [067] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; (b) grape seed extract; and (c) pectin, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:5.5. [068] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) pectin, wherein the
15 jamun seed extract to the grape seed extract w/w ratio in said composition is 1:8.3.
[069] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) pectin, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:12.5. [070] In an embodiment of the present disclosure, there is provided a composition
20 comprising: (a) jamun seed extract; (b) grape seed extract; and (c) pectin, wherein the
jamun seed extract to the grape seed extract w/w ratio in said composition is 1:33.3. [071] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the
25 jamun seed extract to the grape seed extract w/w ratio in said composition is in the
range of 1:2 to 1:35.
[072] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin;
12

and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the
jamun seed extract to the grape seed extract w/w ratio in said composition is 1:2.08.
[073] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin;
5 and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the
jamun seed extract to the grape seed extract w/w ratio in said composition is 1:5.5. [074] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the
10 jamun seed extract to the grape seed extract w/w ratio in said composition is 1:8.3.
[075] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:12.5.
15 [076] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:33.3. [077] In an embodiment of the present disclosure, there is provided a composition
20 comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin;
and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is in the range of 1:2:80-1:35:335. [078] In an embodiment of the present disclosure, there is provided a composition
25 comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin;
and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is 1:2.08:83.33.
13

[079] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin;
and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the
jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said
5 composition is 1:5.5:111.1.
[080] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said
10 composition is 1:8.33:133.33.
[081] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said
15 composition is 1:12.5:166.7.
[082] In an embodiment of the present disclosure, there is provided a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said
20 composition is 1:33.3:333.3.
[083] In an embodiment of the present disclosure, there is provided a method for preparation of a composition comprising: (a) jamun seed extract; and (b) grape seed extract, said method comprising: (a) obtaining jamun seed extract; (b) obtaining grape seed extract; and (c) contacting the jamun seed extract and the grape seed extract to
25 obtain a composition, wherein jamun seed extract to grape seed extract w/w ratio in
said composition is in the range of 1:2 to 1:35.
[084] In an embodiment of the present disclosure, there is provided a method for preparation of a composition comprising: (a) jamun seed extract; and (b) grape seed
14

extract, said method comprising: (a) obtaining jamun seed extract; (b) obtaining grape
seed extract; and (c) contacting jamun seed extract and grape seed extract to obtain
said composition, wherein the jamun seed extract to the grape seed extract w/w ratio
in said composition is 1:2.08.
5 [085] In an embodiment of the present disclosure, there is provided a method for
preparation of a composition comprising: (a) jamun seed extract; and (b) grape seed extract, said method comprising the steps of: (a) obtaining jamun seed extract; (b) obtaining grape seed extract; and (c) contacting the jamun seed extract and the grape seed extract to obtain said composition, wherein jamun seed extract to grape seed
10 extract w/w ratio in said composition is 1:5.5.
[086] In an embodiment of the present disclosure, there is provided a method for preparation of a composition comprising: (a) jamun seed extract; and (b) grape seed extract, said method comprising the steps of: (a) obtaining jamun seed extract; (b) obtaining grape seed extract; and (c) contacting jamun seed extract and grape seed
15 extract to obtain said composition, wherein the jamun seed extract to the grape seed
extract w/w ratio in said composition is 1:8.3.
[087] In an embodiment of the present disclosure, there is provided a method for preparation of a composition comprising: (a) jamun seed extract; and (b) grape seed extract, said method comprising the steps of: (a) obtaining jamun seed extract; (b)
20 obtaining grape seed extract; and (c) contacting jamun seed extract and grape seed
extract to obtain said composition, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:12.5.
[088] In an embodiment of the present disclosure, there is provided a method for preparation of a composition comprising: (a) jamun seed extract; and (b) grape seed
25 extract, said method comprising the steps of: (a) obtaining jamun seed extract; (b)
obtaining grape seed extract; and (c) contacting jamun seed extract and grape seed extract to obtain said composition, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:33.3.
15

[089] In an embodiment of the present disclosure, there is provided a method for
preparation of a composition comprising: (a) jamun seed extract; (b) grape seed
extract; (c) resistant maltodextrin, said method comprising: (i) obtaining jamun seed
extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; and
5 (iv) contacting jamun seed extract, grape seed extract, and resistant maltodextrin to
obtain said composition, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is in the range of 1:2 to 1:35.
[090] In an embodiment of the present disclosure, there is provided a method for preparation of a composition comprising: (a) jamun seed extract; (b) grape seed
10 extract; (c) resistant maltodextrin, said method comprising: (i) obtaining jamun seed
extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; and (iv) contacting jamun seed extract, grape seed extract, and resistant maltodextrin to obtain said composition, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:2.08.
15 [091] In an embodiment of the present disclosure, there is provided a method for
preparation of a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin, said method comprising: (i) obtaining jamun seed extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; and (iv) contacting jamun seed extract, grape seed extract, and resistant maltodextrin to
20 obtain said composition, wherein the jamun seed extract to the grape seed extract w/w
ratio in said composition is 1:5.5.
[092] In an embodiment of the present disclosure, there is provided a method for preparation of a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin, said method comprising: (i) obtaining jamun seed
25 extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; and
(iv) contacting jamun seed extract, grape seed extract, and resistant maltodextrin to obtain said composition, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:8.3.
16

[093] In an embodiment of the present disclosure, there is provided a method for
preparation of a composition comprising: (a) jamun seed extract; (b) grape seed
extract; (c) resistant maltodextrin, said method comprising: (i) obtaining jamun
extract; (ii) obtaining grape extract; (iii) obtaining resistant maltodextrin; and (iv)
5 contacting jamun seed extract, grape seed extract, and resistant maltodextrin to obtain
said composition, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:12.5.
[094] In an embodiment of the present disclosure, there is provided a method for preparation of a composition comprising: (a) jamun seed extract; (b) grape seed
10 extract; (c) resistant maltodextrin, said method comprising: (i) obtaining jamun seed
extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; and (iv) contacting jamun seed extract, grape seed extract, and resistant maltodextrin to obtain said composition, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:33.3.
15 [095] In an embodiment of the present disclosure, there is provided a method for
preparation of a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin, said method comprising: (i) obtaining jamun seed extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; and (iv) contacting jamun seed extract, grape seed extract, and resistant maltodextrin to
20 obtain said composition, wherein the jamun seed extract to the grape seed extract to
resistant maltodextrin w/w ratio in said composition is in the range of 1:2:80-1:35:335.
[096] In an embodiment of the present disclosure, there is provided a method for preparation of a composition comprising: (a) jamun seed extract; (b) grape seed
25 extract; (c) resistant maltodextrin, said method comprising: (i) obtaining jamun seed
extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; and (iv) contacting jamun seed extract, grape seed extract, and resistant maltodextrin to
17

obtain said composition, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is 1:2.08:83.33.
[097] In an embodiment of the present disclosure, there is provided a method for
preparation of a composition comprising: (a) jamun seed extract; (b) grape seed
5 extract; (c) resistant maltodextrin, said method comprising: (i) obtaining jamun seed
extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; and (iv) contacting jamun seed extract, grape seed extract, and resistant maltodextrin to obtain said composition, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is 1:5.5:111.1.
10 [098] In an embodiment of the present disclosure, there is provided a method for
preparation of a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin, said method comprising: (i) obtaining jamun seed extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; and (iv) contacting jamun seed extract, grape seed extract, and resistant maltodextrin to
15 obtain said composition, wherein the jamun seed extract to the grape seed extract to
resistant maltodextrin w/w ratio in said composition is 1:8.3:133.33.
[099] In an embodiment of the present disclosure, there is provided a method for preparation of a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin, said method comprising: (i) obtaining jamun
20 extract; (ii) obtaining grape extract; (iii) obtaining resistant maltodextrin; and (iv)
contacting jamun seed extract, grape seed extract, and resistant maltodextrin to obtain said composition, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is 1:12.5:166.7. [100] In an embodiment of the present disclosure, there is provided a method for
25 preparation of a composition comprising: (a) jamun seed extract; (b) grape seed
extract; (c) resistant maltodextrin, said method comprising: (i) obtaining jamun seed extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; and (iv) contacting jamun seed extract, grape seed extract, and resistant maltodextrin to
18

obtain said composition, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is 1:33.3:333.3.
[101] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin;
5 (d) at least one carrier, at least one diluent and at least one excipient, said method
comprising the steps of: (i) obtaining jamun seed extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; (iv) obtaining at least one carrier; (v) obtaining at least one diluent; (vi) obtaining at least one excipient; and (vii) contacting jamun seed extract, grape seed extract, resistant maltodextrin, at least one
10 carrier, at least one diluent, and at least one excipient to obtain said composition,
wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is in the range of 1:2 to 1:35.
[102] In an embodiment of the present disclosure, there is provided method for preparation of a composition comprising: (a) jamun seed extract; (b) grape seed
15 extract; (c) resistant maltodextrin; and (d) at least one carrier, at least one diluent, and
at least one excipient, said method comprising the steps of: (i) obtaining jamun seed extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; (iv) obtaining at least one carrier; (v) obtaining at least one diluent; (vi) obtaining at least one excipient; and (vii) contacting jamun seed extract, grape seed extract, resistant
20 maltodextrin, at least one carrier, at least one diluent, and at least one excipient to
obtain said composition, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:2.08.
[103] In an embodiment of the present disclosure, there is provided a method for preparation of a composition comprising: (a) jamun seed extract; (b) grape seed
25 extract; (c) resistant maltodextrin; and (d) at least one carrier at least one diluent, and
at least one excipient, said method comprising the steps of: (i) obtaining jamun seed extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; (iv) obtaining at least one carrier; (v) obtaining at least one diluent; (vi) obtaining at least
19

one excipient; and (vii) contacting jamun seed extract, grape seed extract, resistant
maltodextrin, at least one carrier, at least one diluent, and at least one excipient to
obtain said composition, wherein the jamun seed extract to the grape seed extract w/w
ratio in said composition is 1:5.5.
5 [104] In an embodiment of the present disclosure, there is provided method for
preparation of a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, said method comprising the steps of: (i) obtaining jamun seed extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; (iv)
10 obtaining at least one carrier; (v) obtaining at least one diluent; (vi) obtaining at least
one excipient; and (vii) contacting jamun extract, grape extract, resistant maltodextrin, at least one carrier, at least one diluent, and at least one excipient to obtain said composition, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:8.3.
15 [105] In an embodiment of the present disclosure, there is provided method for
preparation of a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier, at least one diluent, and at least one excipient, said method comprising the steps of: (i) obtaining jamun seed extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; (iv)
20 obtaining at least one carrier; (v) obtaining at least one diluent; (vi) obtaining at least
one excipient; and (vii) contacting jamun seed extract, grape seed extract, resistant maltodextrin, at least one carrier, at least one diluent, and at least one excipient to obtain said composition, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:12.5.
25 [106] In an embodiment of the present disclosure, there is provided method for
preparation of a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier, at least one diluent, and at least one excipient, said method comprising the steps of: (i) obtaining jamun seed
20

extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; (iv)
obtaining at least one carrier; (v) obtaining at least one diluent; (vi) obtaining at least
one excipient; and (vii) contacting jamun seed extract, grape seed extract, resistant
maltodextrin, at least one carrier, at least one diluent, and at least one excipient to
5 obtain said composition, wherein the jamun seed extract to the grape seed extract w/w
ratio in said composition is 1:33.3.
[107] In an embodiment of the present disclosure, there is provided method for preparation of a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; (d) at least one carrier, at least one diluent, and at
10 least one excipient, said method comprising the steps of: (i) obtaining jamun seed
extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; (iv) obtaining at least one carrier; (v) obtaining at least one diluent; (vi) obtaining at least one excipient; and (vii) contacting jamun seed extract, grape seed extract, resistant maltodextrin, at least one carrier, at least one diluent, and at least one excipient to
15 obtain said composition, wherein the jamun seed extract to the grape seed extract to
resistant maltodextrin w/w ratio in said composition is in the range of 1:2:80 to 1:35:335.
[108] In an embodiment of the present disclosure, there is provided method for preparation of a composition comprising: (a) jamun seed extract; (b) grape seed
20 extract; (c) resistant maltodextrin; and (d) at least one carrier, at least one diluent, and
at least one excipient, said method comprising the steps of: (i) obtaining jamun seed extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; (iv) obtaining at least one carrier; (v) obtaining at least one diluent; (vi) obtaining at least one excipient; and (vii) contacting jamun seed extract, grape seed extract, resistant
25 maltodextrin, at least one carrier, at least one diluent, and at least one excipient to
obtain said composition, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is 1:2.08:83.33.
21

[109] In an embodiment of the present disclosure, there is provided method for
preparation of a composition comprising: (a) jamun seed extract; (b) grape seed
extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent, and
at least one excipient, said method comprising the steps of: (i) obtaining jamun seed
5 extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; (iv)
obtaining at least one carrier; (v) obtaining at least one diluent; (vi) obtaining at least one excipient; and (vii) contacting jamun seed extract, grape seed extract, resistant maltodextrin, at least one carrier, at least one diluent, and at least one excipient to obtain said composition, wherein the jamun seed extract to the grape seed extract to
10 resistant maltodextrin w/w ratio in said composition is 1:5.5:111.1.
[110] In an embodiment of the present disclosure, there is provided method for preparation of a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, said method comprising the steps of: (i) obtaining jamun seed
15 extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; (iv)
obtaining at least one carrier; (v) obtaining at least one diluent; (vi) obtaining at least one excipient; and (vii) contacting jamun extract, grape extract, resistant maltodextrin, at least one carrier, at least one diluent, and at least one excipient to obtain said composition, wherein the jamun seed extract to the grape seed extract to
20 resistant maltodextrin w/w ratio in said composition is 1:8.3:133.33.
[111] In an embodiment of the present disclosure, there is provided method for preparation of a composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, said method comprising the steps of: (i) obtaining jamun seed
25 extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; (iv)
obtaining at least one carrier; (v) obtaining at least one diluent; (vi) obtaining at least one excipient; and (vii) contacting jamun seed extract, grape seed extract, resistant maltodextrin, at least one carrier, at least one diluent, and at least one excipient to
22

obtain said composition, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is 1:12.5:166.7.
[112] In an embodiment of the present disclosure, there is provided method for
preparation of a composition comprising: (a) jamun seed extract; (b) grape seed
5 extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and
at least one excipient, said method comprising the steps of: (i) obtaining jamun seed extract; (ii) obtaining grape seed extract; (iii) obtaining resistant maltodextrin; (iv) obtaining at least one carrier; (v) obtaining at least one diluent; (vi) obtaining at least one excipient; and (vii) contacting jamun seed extract, grape seed extract, resistant
10 maltodextrin, at least one carrier, at least one diluent, and at least one excipient to
obtain said composition, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is 1:33.3:333.3.
[113] In an embodiment of the present disclosure, there is provided a composition to inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; and
15 (b) grape seed extract, wherein the jamun seed extract to the grape seed extract w/w
ratio in said composition is in the range of 1:2 to 1.35.
[114] In an embodiment of the present disclosure, there is provided a composition to inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; and (b) grape seed extract, wherein jamun seed extract to grape seed extract w/w ratio in
20 said composition is 1:2.08.
[115] In an embodiment of the present disclosure, there is provided a composition to inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; and (b) grape seed extract, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:5.5.
25 [116] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; and (b) grape seed extract, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:8.3.
23

[117] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; and
(b) grape seed extract, wherein the jamun seed extract to the grape seed extract w/w
ratio in said composition is 1:12.5.
5 [118] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; and (b) grape seed extract, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:33.3. [119] In an embodiment of the present disclosure, there is provided a composition to
10 inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b)
grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is in the range of 1:2 to 1:35. [120] In an embodiment of the present disclosure, there is provided a composition to inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b)
15 grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to
the grape seed extract w/w ratio in said composition is 1:2.08.
[121] In an embodiment of the present disclosure, there is provided a composition to inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to
20 the grape seed extract w/w ratio in said composition is 1:5.5.
[122] In an embodiment of the present disclosure, there is provided a composition to inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:8.3.
25 [123] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to the grape seed extract w/w ratio in said composition is 1:12.5.
24

[124] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b)
grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to
the grape seed extract w/w ratio in said composition is 1:33.3.
5 [125] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is in the range of 1:2:85.
10 [126] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is 1:2.08:83.33.
15 [127] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is 1:5.5:111.1.
20 [128] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is 1:8.3:133.33.
25 [129] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b) grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to
25

the grape seed extract to resistant maltodextrin w/w ratio in said composition is
1:12.5:166.7.
[130] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b)
5 grape seed extract; and (c) resistant maltodextrin, wherein the jamun seed extract to
the grape seed extract to resistant maltodextrin w/w ratio in said composition is
1:33.3:333.3.
[131] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b)
10 grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one
diluent; and at least one excipient, wherein the jamun seed extract to the grape seed
extract w/w ratio in said composition is in the range of 1:2 to 1:35.
[132] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b)
15 grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one
diluent; and at least one excipient, wherein the jamun seed extract to the grape seed
extract w/w ratio in said composition is 1:2.08.
[133] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b)
20 grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one
diluent; and at least one excipient, wherein the jamun seed extract to the grape seed
extract w/w ratio in said composition is 1:5.5.
[134] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b)
25 grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one
diluent; and at least one excipient, wherein the jamun seed extract to the grape seed
extract w/w ratio in said composition is 1:8.3.
26

[135] In an embodiment of the present disclosure, there is provided a composition to
inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b)
grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one
diluent; and at least one excipient, wherein the jamun seed extract to the grape seed
5 extract w/w ratio in said composition is 1:12.5.
[136] In an embodiment of the present disclosure, there is provided a composition to inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the jamun seed extract to the grape seed
10 extract w/w ratio in said composition is 1:33.3.
[137] In an embodiment of the present disclosure, there is provided a composition to inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the jamun seed extract to the grape seed
15 extract to resistant maltodextrin w/w ratio in said composition is in the range of
1:2:80 to 1:35:335.
[138] In an embodiment of the present disclosure, there is provided a composition to inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one
20 diluent; and at least one excipient, wherein the jamun seed extract to the grape seed
extract to resistant maltodextrin w/w ratio in said composition is 1:2.08:83.33. [139] In an embodiment of the present disclosure, there is provided a composition to inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b) grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one
25 diluent; and at least one excipient, wherein the jamun seed extract to the grape seed
extract to resistant maltodextrin w/w ratio in said composition is 1:5.5:111.1. [140] In an embodiment of the present disclosure, there is provided a composition to inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b)
27

grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one
diluent; and at least one excipient, wherein the jamun seed extract to the grape seed
extract to resistant maltodextrin w/w ratio in said composition is 1:8.3:133.33.
[141] In an embodiment of the present disclosure, there is provided a composition to
5 inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b)
grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is 1:12.5:166.7. [142] In an embodiment of the present disclosure, there is provided a composition to
10 inhibit alpha glucosidase, said composition comprising: (a) jamun seed extract; (b)
grape seed extract; (c) resistant maltodextrin; and (d) at least one carrier; at least one diluent; and at least one excipient, wherein the jamun seed extract to the grape seed extract to resistant maltodextrin w/w ratio in said composition is 1:33.3:333.3. [143] Although the subject matter has been described with reference to specific
15 embodiments, this description is not meant to be construed in a limiting sense.
Various modifications of the disclosed embodiments, as well as alternate embodiments of the subject matter, will become apparent to persons skilled in the art upon reference to the description of the subject matter. It is therefore contemplated that such modifications can be made without departing from the spirit or scope of the
20 present subject matter as defined.
EXAMPLES
[144] The disclosure will now be illustrated with working examples, which is
intended to illustrate the working of disclosure and not intended to take restrictively
25 to imply any limitations on the scope of the present disclosure. Unless defined
otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described
28

herein can be used in the practice of the disclosed methods and compositions, the
exemplary methods, devices and materials are described herein. It is to be
understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may vary.
5 Example 1
Materials and methods
[145] Both jamun and grape seed extracts were procured from SV Agro India
limited. Both extracts were obtained in powdered form and dissolved in water.
Fingerprint analysis for the two extracts (as obtained from certificate of analysis)
10 identified proanthocyanidins as the active ingredient for grape seed extract
comprising 95% of the extract. The active ingredient identified for grape seed extract was polyphenols and the extract used comprises 5% polyphenols.
Example 2
Alpha glucosidase assay
15 [146] The enzyme alpha glucosidase was prepared using 500 mg of rat intestinal
acetone powder was weighed and to it 10 mL of ice cold assay buffer was added. The solution was vortexed gently and allowed to stand in ice for 2 minutes. The mix was then centrifuged at 8000 rpm for 8 minutes. The supernatant was transferred to a fresh tube and the pellet discarded. The supernatant aliquots were used as the enzyme
20 solution. The above procedure was carried out at 4°C and all the enzyme solution was
stored at -20°C.
[147] The alpha glucosidase assay was carried out in a total reaction volume of 200 µL comprising assay buffer, substrate, inhibitors and the enzyme. 0.1 M phosphate buffer at pH 7 was used as the assay buffer as well as the solvent for enzyme and
25 substrate solutions. The assay components were added in their respective volumes
and the reaction was started by addition of the enzyme. The reaction was carried out at 37 °C for one hour with the absorbance readings being measured every one minute. The assay was based on the action of alpha-glucosidase enzyme which acts on alpha-
29

1, 4 glucosidic bonds. The substrate used in the assay was para-nitrophenol tagged
alpha-D-glucopyranoside. The enzyme acts on the substrate and p-nitrophenol is
released, absorbance of which was measured calorimetrically at 410 nm. Percentage
inhibition was calculated as, % Inhibition = (Absorbance Control – Absorbance
5 Test)/ Absorbance Control × 100.
Example 3
Determination of efficacious concentration of jamun seed extract and grape seed
extract on inhibition of alpha glucosidase
[148] To determine efficacious concentration of jamun seed extract on alpha
10 glucosidase, varying concentrations of jamun seed extract were tested. The
concentration ranges from 0.15 – 0.01875 mg/ml of jamun seed extract were tested. Figure 1 depicts the effect of different concentrations of jamun seed extract on inhibition of alpha glucosidase. As evident from Figure 1, the highest percent inhibition was obtained at a concentration of 0.15 mg/ml which was 42.57 %,
15 followed by the concentration of 0.075 mg/ml which gave a percent inhibition at
28.44%. It was observed that with decreasing concentrations of jamun seed extract, there was a decrease in the percent inhibition of alpha glucosidase (Figure 1). Thus, these results demonstrate that jamun seed extract possess the ability to inhibit alpha glucosidase. Based on these results 0.15 mg/ml concentration was selected for further
20 experimentation.
[149] Similarly, the efficacious concentration of grape seed extract for inhibiting alpha glucosidase activity was determined. The different concentrations tested ranged from 2.5 – 0.31 mg/ml. In this case also, the highest percent inhibition was obtained at the highest concentration of 2.5 mg/ml (Figure 2). With a decrease in the
25 concentration of grape extract, there was a corresponding decrease in the percent
inhibition of alpha glucosidase. Highest percent inhibition of 53.43 % was obtained at a concentration of 2.5 mg/ml, followed by 30.38% obtained at a concentration of 1.25 mg/ml. Thus, as in the case of jamun seed extract, grape seed extract also possesses
30

the ability to inhibit alpha glucosidase activity. Based on these results 1.25 mg/ml concentration of grape seed extract was selected for further experimentation.
Example 4
Evaluation of a combination of jamun seed extract and grape seed extract on
5 inhibition of alpha glucosidase
[150] Based on the results obtained in the above experiments, various combinations of jamun seed extract and grape seed extract were checked for their effect on the inhibition of alpha glucosidase. The ratios used are described in Table 1 below:
Table 1

Ratio (as per percentage of
max concentration) Concentration of
jamun seed extract (mg/mL) Concentration of
grape seed extract (mg/mL) 10
w/w ratio (as per concentration)
(100:0) 0.15 0 -
(80:20) 0.12 0.25 1:2.08
(60:40) 0.09 0.5 1: 5.5
(50:50) 0.075 0.625 1: 8.3
(40:60) 0.06 0.75 1: 12.5 15
(20:80) 0.03 1 1: 33.3
(0:100) 0 1.25 -
in the Table above, the maximum concentration used for the actives was 0.15 mg/ml for jamun seed extract and 1.25 mg/ml for grape seed extract. The subsequent
20 dilutions were calculated as a percentage of the maximum concentrations, for
instance a 80:20 ratio of jamun seed extract: grape seed extract represents 80% of 0.15 mg/ml and 20% of 1.25 mg/ml of grape extract. The concentrations thus calculated for this ratio are 0.12 mg/ml of jamun seed extract and 0.25 mg/ml of grape seed extract.
25 [152] Unexpectedly, combinations of jamun seed extract and grape seed extract
demonstrated distinct synergism in inhibiting the activity of alpha glucosidase. The ratios in the range of 1:2 – 1:35 of jamun seed extract to grape seed extract exhibited higher percent inhibition than the extracts in isolation (Figure 4). Jamun seed extract
31

and grape seed extract alone had percent inhibition of 42.02 % and 30.34 %,
respectively. On the other hand, the combinations of the ingredients at a 1:2.08,
1:5.5, 1:8.3, 1:12.5 and 1:33.3 ratio of jamun seed extract to grape seed extract
exhibited percent inhibition of 52.96 %, 53.23 %, 56.99 %, 54.23 % and 60.01 %
5 respectively. The highest percent inhibition was obtained at a w/w ratio of 1:33.3.
The combinatorial effect of the extracts, as compared with the effect of extracts in isolation, were much higher than what could be expected from mere admixture of the two, and thus, distinct synergism is apparent in the combination of jamun seed extract and grape seed extract.
10 Example 4
Inhibitory effect of different polysaccharides and gums
[153] Bulking agents often serve to provide a filling effect after eating and could help reduce the calorific intake in a daily diet. Thus, a group of bulking agents were tested to determine whether the effect of these agents was restricted to just providing
15 a filling effect or could potentially influence sugar metabolism. Different bulking
agents analyzed included resistant maltodextrin at a weight percentage of 1 %, gum arabic at weight percentage of 1 %, guar gum at a weight percentage of 0.1 %, and pectin at a weight percentage of 0.1 % (Figure 4). Here, lower concentration of guar gum and pectin were tried since their solubility was very low. When these bulking
20 agents were compared, the highest percent inhibition of alpha glucosidase was
obtained with resistant maltodextrin at a weight percentage of 1 % and was also not limited by its solubility. Therefore, it was selected as the bulking agent for further experiments.
25 Example 6
Evaluation of a combination of jamun seed extract, grape seed extract and resistant maltodextrin on inhibition of alpha glucosidase
32

[154] Following the observation obtained with the combination of extracts on the
inhibition of alpha glucosidase, combinations of these extracts with maltodextrin,
acting as a bulking agent was also analyzed. Unexpectedly and surprisingly, a higher
percent inhibition of alpha glucosidase was obtained when the jamun and grape seed
5 extract was combined with maltodextrin at a final concentration of 1% maltodextrin
(Figure 5). The w/w ratio range of 1:2 – 1:35 of jamun seed extract to grape seed extract exhibited much higher percentage inhibition of alpha glucosidase as compared to the jamun and grape seed extract was used without resistant maltodextrin. Jamun seed extract and grape seed extract alone with a combination of maltodextrin were
10 giving a percent inhibition of 50.96 % and 37.01 % respectively (Figure 5). On the
other hand, a ratio of 1:2.08, 1:5.5, 1:8.3, 1:12.5 and 1:33.3 of jamun seed extract to grape seed extract in combination with 1 % maltodextrin showed a percent inhibition of 62.70%, 65.76%, 55.90%, 68.06%, 68.92% respectively. The combinatorial effect of the extracts with resistant maltodextrin as compared to that without maltodextrin
15 are much higher than what could be expected by mere admixture of a bulking agent.
Thus, there is distinct synergism is apparent in the combination of jamun seed extract, grape seed extract and maltodextrin.
[155] Overall, the present disclosure provides a composition of jamun seed extract and grape seed extract, exhibits a surprising and unexpected synergistic effect on
20 alpha glucosidase activity. Further, the synergistic effect is enhanced on the addition
of resistant maltodextrin, which itself was an unexpected find. Thus, the combination of jamun seed extract and grape seed extract shows synergism in inhibiting alpha glucosidase with and without resistant maltodextrin and thus can be used for the management of metabolic diseases. Further, the identification of this novel
25 combination for the management of metabolic and lifestyle-related diseases cannot be
arrived at without undue experimentation, and is thus not obvious to a person skilled in the art.
33

I/We Claim:
1. A composition comprising:
(a) jamun seed extract; and
(b) grape seed extract,
5 wherein the jamun seed extract to the grape seed extract w/w ratio in said
composition is in the range of 1:2 to 1:35.
2. The composition as claimed in claim 1, further comprising at least one
bulking agent selected from a group consisting of resistant maltodextrin,
pectin, gelatin, and gums.
10 3. The composition as claimed in claim 1, wherein said bulking agent is resistant
maltodextrin.
4. The composition as claimed in claim 1, wherein the jamun seed extract to the
grape seed extract w/w ratio in said composition is 1:33.3.
5. The composition as claimed in any of the claims 1-4, further comprising, at
15 least one carrier, at least one diluent, and at least one excipient.
6. A method for preparation of a composition as claimed in claim 1, said method
comprising:
(a) obtaining jamun seed extract;
(b) obtaining grape seed extract; and
20 (c) contacting the jamun seed extract and the grape seed extract to obtain said
composition.
7. A method for preparation of a composition as claimed in any of the claims 1-
4, said method comprising:
(a) obtaining jamun seed extract;
25 (b) obtaining grape seed extract;
(c) obtaining resistant maltodextrin; and
(d) contacting the jamun seed extract, the grape seed extract, and the resistant
maltodextrin to obtain said composition.
34

8. A method for preparation of a composition as claimed in claim 5, said method
comprising:
(a) obtaining jamun seed extract;
(b) obtaining grape seed extract;
5 (c) obtaining resistant maltodextrin;
(d) obtaining at least one carrier;
(e) obtaining at least one diluent;
(f) obtaining at least one excipient; and
(g) contacting the jamun seed extract, the grape seed extract, the resistant 10 maltodextrin, the at least one carrier, the at least one diluent, and the at
least one excipient to obtain said composition.
9. The composition as claimed in any of claims 1-5, wherein said composition
inhibits alpha glucosidase.
35