FIELD OF INVENTION
[001] The present disclosure relates to the field of formulations for disease management in general and metabolic diseases in particular. There is provided a composition comprising pomegranate fruit extract and celery seed extract, which at specific ratios and concentrations can be utilised to treat metabolic diseases.
BACKGROUND OF INVENTION
[002] Lifestyle diseases are increasingly becoming a major health issue in
industrialized countries around the world. These diseases include a host of
metabolism- related disorders including diabetes, obesity, and heart disease among
others.
[003] Obesity is one of the leading metabolic diseases and the primary cause of
several others. It is a condition where the body stores excess fat and is the result of a
sedentary lifestyle and excessive intake of food. The development of obesity is linked
to several other metabolic diseases including heart disease, type 2 diabetes,
obstructive sleep apnea, certain types of cancer, and osteoarthritis [Haslam and
James, "Obesity",2005 Lancet 366 (9492): 1197–209]. India ranks third in top ten
countries with highest number of obese people. [Marie et al., The Lancet, 2014,
6736(14)].
[004] Diabetes is also one of the most commonly occurring chronic metabolic
diseases characterized by hyperglycaemia. India ranks second for people with
diabetes in top ten countries (IDF diabetes Atlas 5th edition, 2012 update). The
disease is characterized by high blood glucose levels owing to reduced insulin levels.
[005] One of the enzymes involved in the absorption of carbohydrates from the gut
is alpha-glucosidase. Inhibiting the activity of this enzyme provides a possible
solution for the treatment of such metabolic diseases [van de Laar et al., Vasc. Health
Risk Manag, 2008; 4(6); Okada K et al., Hepatol. Res., 2009; 39(5)].
[006] Several studies are ongoing to find solutions to manage both obesity and
diabetes. Among the possible measures for the management of these diseases is using
natural and herbal alternatives to drug therapy.

[007] In conclusion, there is a need to find novel and curative and preventive measures for the treatment of emerging lifestyle diseases as they become an increasing problem of industrialised societies.
SUMMARY OF INVENTION
[008] In an aspect of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; and (b) celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4-4:1.
[009] In an aspect of present disclosure, there is provided a method of preparing a composition comprising: (a) pomegranate fruit extract; and (b) celery seed extract, said method comprising: (i) obtaining pomegranate fruit extract; (ii) obtaining celery seed extract; and (iii) contacting pomegranate fruit extract, celery seed extract to obtain said composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4 – 4:1.
[0010] These and other features, aspects, and advantages of the present subject matter will be better understood with reference to the following description and appended claims. This summary is provided to introduce a selection of concepts in a simplified form. This summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used to limit the scope of the claimed subject matter.
BRIEF DESCRIPTION OF ACCOMPANYING DRAWINGS
[0011] The following drawings form a part of the present specification and are
included to further illustrate aspects of the present disclosure. The disclosure may be
better understood by reference to the drawings in combination with the detailed
description of the specific embodiments presented herein.
[0012] Figure 1 shows the percentage inhibition of alpha-glucosidase activity by
pomegranate fruit extract and celery seed extract, in accordance with an embodiment
of the present disclosure.

[0013] Figure 2 shows the percentage inhibition of alpha-glucosidase activity by the combination of pomegranate fruit extract and celery seed extract at different w/w ratios, in accordance with an embodiment of the present disclosure.
DETAILED DESCRIPTION OF THE INVENTION
[0014] The present disclosure relates to a novel composition comprising pomegranate fruit extract and celery seed extract, which at specific ratios and concentrations can be used for the management of obesity and diabetes by the regulation of alpha-glucosidase enzymatic activity.
[0015] Those skilled in the art will be aware that the present disclosure is subject to variations and modifications other than those specifically described. It is to be understood that the present disclosure includes all such variations and modifications. The disclosure also includes all such steps, features, compositions, and compounds referred to or indicated in this specification, individually or collectively, and any and all combinations of any or more of such steps or features. Definitions
[0016] For convenience, before further description of the present disclosure, certain terms employed in the specification, and examples are delineated here. These definitions should be read in the light of the remainder of the disclosure and understood as by a person of skill in the art. The terms used herein have the meanings recognized and known to those of skill in the art, however, for convenience and completeness, particular terms and their meanings are set forth below.
[0017] The articles “a”, “an” and “the” are used to refer to one or to more than one (i.e., to at least one) of the grammatical object of the article.
[0018] The terms “comprise” and “comprising” are used in the inclusive, open sense, meaning that additional elements may be included. It is not intended to be construed as “consists of only”.
[0019] Throughout this specification, unless the context requires otherwise the word “comprise”, and variations such as “comprises” and “comprising”, will be understood

to imply the inclusion of a stated element or step or group of element or steps but not
the exclusion of any other element or step or group of element or steps.
[0020] The term “including” is used to mean “including but not limited to”.
“Including” and “including but not limited to” are used interchangeably.
[0021] The term “metabolic diseases” will be understood to refer to the diseases
including obesity, type 2 diabetes, diabetes, cardio vascular diseases and diabetes
mediated complications.
[0022] Carriers are substances that serve as mechanisms to improve the delivery and
the effectiveness of drugs.
[0023] A diluent (also referred to as filler, dilutant, or thinner) is a diluting agent.
[0024] An excipient is an inactive substance that serves as the vehicle or medium for
a drug or other active substance. Excipients include colouring agents, humectants,
preservatives, emollients, and combinations thereof.
[0025] Unless defined otherwise, all technical and scientific terms used herein have
the same meaning as commonly understood by one of ordinary skill in the art to
which this disclosure belongs. Although any methods and materials similar or
equivalent to those described herein can be used in the practice or testing of the
disclosure, the preferred methods, and materials are now described. All publications
mentioned herein are incorporated herein by reference.
[0026] A major emerging problem in industrialized countries is the development of
metabolic diseases. Among them, obesity and type 2 diabetes are the most prevalent
conditions and we are yet to find any definitive cures or treatments for the
management of these diseases. The present disclosure provides herein, a synergistic
composition comprising of pomegranate fruit extract and celery seed extract that is
beneficial in treating diabetes, obesity and its complications.
[0027] Punica granatum, also known as pomegranate, belongs to the family
Punicaceae. The fruit of the Punica granatum is known to have beneficial effects for
the treatment of acidosis, dysentery, microbial infections, diarrhoea, helminth

infection, haemorrhage and respiratory pathologies. Punica granatum is rich in polyphenols [Reza et al., 2012; Iran J Pharm Res.; 11(2)].
[0028] Apium graveolens commonly known as celery, belongs to family Apiaceae. Celery seed is commonly used as a spice. Celery seed is known to have uses in the treatment of colds, flu, water retention, poor digestion, different types of arthritis, and certain diseases of the liver and spleen [Hassanpour, et al., Journal of Medicinal Food, 2013; 16(6)].
[0029] The present disclosure is not to be limited in scope by the specific embodiments described herein, which are intended for the purposes of exemplification only. Functionally-equivalent products, compositions, and methods are clearly within the scope of the disclosure, as described herein.
[0030] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; and (b) celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4-4:1.
[0031] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; and (b) celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is 1:4. [0032] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; and (b) celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is 2:3. [0033] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; and (b) celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is 1:1. [0034] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; and (b) celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is 3:2.

[0035] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; and (b) celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is 4:1. [0036] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract having a weight percentage in the range of 0.2%-0.8% in said composition; and (b) celery seed extract having a weight percentage in the range of 0.2%-0.8% in said composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4-4:1. [0037] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract having a weight percentage in the range of 0.2%-0.8% in said composition ; and (b) celery seed extract having a weight percentage in the range of 0.2%-0.8% in said composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4-4:1, wherein said composition treats metabolic diseases, including diabetes, obesity and complications arising from said diseases, through inhibition of alpha-glucosidase activity.
[0038] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract having a weight percentage in the range of 0.2%-0.8% in said composition; and (b) celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4-4:1.
[0039] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; and (b) celery seed extract having a weight percentage in the range of 0.2%-0.8% in said composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4-4:1. [0040] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract having a weight percentage of 0.2% in said composition; and (b) celery seed extract having a weight percentage of 0.8% in said

composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in
said composition is in the range of 1:4- 4:1.
[0041] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) pomegranate fruit extract having a weight percentage of 0.2% in said
composition; and (b) celery seed extract, wherein pomegranate fruit extract to celery
seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0042] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) pomegranate fruit extract; and (b) celery seed extract having a weight
percentage of 0.8%, wherein pomegranate fruit extract to celery seed extract w/w
ratio in said composition is in the range of 1:4- 4:1.
[0043] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) pomegranate fruit extract having a weight percentage of 0.6% in said
composition; and (b) celery seed extract having a weight percentage of 0.4% in said
composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in
said composition is in the range of 1:4-4:1.
[0044] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) pomegranate fruit extract having a weight percentage of 0.6% in said
composition; and (b) celery seed extract, wherein pomegranate fruit extract to celery
seed extract w/w ratio in said composition is in the range of 1:4-4:1.
[0045] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) pomegranate fruit extract; and (b) celery seed extract having a weight
percentage of 0.4% in said composition, wherein pomegranate fruit extract to celery
seed extract w/w ratio in said composition is in the range of 1:4-4:1.
[0046] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) pomegranate fruit extract having a weight percentage of 0.8% in said
composition; and (b) celery seed extract having a weight percentage of 0.2% in said
composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in
said composition is 1:4- 4:1.

[0047] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract having a weight percentage of 0.8% in said composition; and (b) celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is 1:4- 4:1.
[0048] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; and (b) celery seed extract having a weight percentage of 0.2% in said composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is 1:4- 4:1.
[0049] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; (b) celery seed extract; (c) carriers, (d) diluents, and (e) excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0050] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; (b) celery seed extract; and (c) carriers, (d) diluents, and (e) excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1, and said composition treats metabolic diseases, including diabetes, obesity and complications arising from said diseases, through inhibition of alpha-glucosidase activity.
[0051] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract having a weight percentage in the range of 0.2%-0.8% in said composition; (b) celery seed extract having a weight percentage in the range of 0.2%-0.8% in said composition; and (c) carriers, (d) diluents, and (e) excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0052] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract having a weight percentage in the range of 0.2%-0.8% in said composition; (b) celery seed extract having a weight percentage in the range of 0.2%-0.8% in said composition; and (c) carriers, (d) diluents, and (e) excipients, , wherein pomegranate fruit extract to celery seed extract w/w ratio in said

composition is in the range of 1:4- 4:1, and said composition treats metabolic diseases, including diabetes, obesity and complications arising from said diseases, through inhibition of alpha-glucosidase activity.
[0053] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract having a weight percentage in the range of 0.2%-0.8% in said composition; (b) celery seed extract; and (c) carriers, (d) diluents, and (e) excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0054] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; (b) celery seed extract having a weight percentage in the range of 0.2%-0.8% in said composition; and (c) carriers, (d) diluents, and (e) excipients, , wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0055] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract having a weight percentage of 0.2%; (b) celery seed extract; and (c) carriers, (d) diluents, and (e) excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0056] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; (b) celery seed extract having a weight percentage of 0.8% in said composition; and (c) carriers, (d) diluents, and (e) excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0057] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract having a weight percentage of 0.6% in said composition; (b) celery seed extract having a weight percentage of 0.4% in said composition; and (c) carriers, (d) diluents, and (e) excipients, , wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4-4:1.

[0058] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract having a weight percentage of 0.6% in said composition; (b) celery seed extract; and (c) (c) carriers, (d) diluents, and (e) excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is 1:4- 4:1.
[0059] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; (b) celery seed extract having a weight percentage of 0.4% in said composition; and (c) (c) carriers, (d) diluents, and (e) excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is 1:4- 4:1.
[0060] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract having a weight percentage of 0.8% in said composition; (b) celery seed extract having a weight percentage of 0.2% in said composition; and (c) carriers, (d) diluents, and (e) excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is 1:4- 4:1. [0061] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract having a weight percentage of 0.8% in said composition; (b) celery seed extract; and (c) (c) carriers, (d) diluents, and (e) excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0062] In an embodiment of the present disclosure, there is provided a composition comprising: (a) pomegranate fruit extract; (b) celery seed extract having a weight percentage of 0.2% in said composition; and (c) carriers, (d) diluents, and (e) excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0063] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; and (c) contacting pomegranate fruit extract and celery

seed extract to obtain said composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0064] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; and (c) contacting pomegranate fruit extract and celery seed extract to obtain said composition, wherein pomegranate fruit extract weight percentage in said composition is in the range of 0.2%-0.8% in said composition; and celery seed extract weight percentage in said composition is in the range of 0.2%-0.8% in said composition; wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0065] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; and (c) contacting pomegranate fruit extract and celery seed extract to obtain said composition, wherein pomegranate fruit extract weight percentage in said composition is in the range of 0.2%-0.8%; and pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1. [0066] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; and (c) contacting pomegranate fruit extract and celery seed extract to obtain said composition, wherein celery seed extract weight percentage in said composition is in the range of 0.2%-0.8%; and pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1. [0067] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; and (c) contacting pomegranate fruit extract and celery seed extract to obtain said composition, wherein said composition comprises: (a) pomegranate fruit extract having a weight percentage of 0.2%; and (b) celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.

[0068] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; and (c) contacting pomegranate fruit extract and celery seed extract to obtain said composition, wherein said composition comprises: (a) pomegranate fruit extract; and (b) celery seed extract having a weight percentage of 0.8%, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0069] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; and (c) contacting pomegranate fruit extract and celery seed extract to obtain said composition, wherein said composition comprises: (a) pomegranate fruit extract having a weight percentage of 0.6%; and (b) celery seed extract having a weight percentage of 0.4%, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1. [0070] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; and (c) contacting pomegranate fruit extract and celery seed extract to obtain said composition, wherein said composition comprises: (a) pomegranate fruit extract having a weight percentage of 0.6%; and (b) celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0071] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; and (c) contacting pomegranate fruit extract and celery seed extract to obtain said composition, wherein said composition comprises: (a) pomegranate fruit extract; and (b) celery seed extract having a weight percentage of 0.4%, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.

[0072] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; and (c) contacting pomegranate fruit extract and celery seed extract to obtain said composition, wherein said composition comprises: (a) pomegranate fruit extract having a weight percentage of 0.8%; and (b) celery seed extract having a weight percentage of 0.2%, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1. [0073] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; and (c) contacting pomegranate fruit extract and celery seed extract to obtain said composition, wherein said composition comprises: (a) pomegranate fruit extract having a weight percentage of 0.8%; and (b) celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0074] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; and (c) contacting pomegranate fruit extract and celery seed extract to obtain said composition, wherein said composition comprises: (a) pomegranate fruit extract; and (b) celery seed extract having a weight percentage of 0.2%, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0075] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; (c) obtaining carriers, diluents, and excipients; and (d) contacting pomegranate fruit extract, celery seed extract, carriers, diluents, and excipients to obtain said composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0076] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising : (a) obtaining pomegranate fruit extract; (b)

obtaining celery seed extract; (c) obtaining carriers, diluents, and excipients; and (d) contacting pomegranate fruit extract and celery seed extract , wherein said composition comprises : (a) pomegranate fruit extract having a weight percentage in the range of 0.2%-0.8% in said composition; and (b) celery seed extract having a weight percentage in the range of 0.2%-0.8% in said composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0077] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; (c) obtaining carriers, diluents, and excipients; and (d) contacting pomegranate fruit extract, celery seed extract and carriers, diluents, and excipients, wherein said composition comprises: (a) pomegranate fruit extract having a weight percentage in the range of 0.2%-0.8% in said composition; and (b) obtaining celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0078] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; (c) obtaining carriers, diluents, and excipients; and (d) contacting pomegranate fruit extract, celery seed extract and carriers, diluents, and excipients, wherein said composition comprises: (a) pomegranate fruit extract and (b) celery seed extract having a weight percentage in the range of 0.2%-0.8% in said composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0079] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; (c) obtaining carriers, diluents, and excipients; and (d) contacting pomegranate fruit extract and celery seed extract, wherein said composition comprises: (a) pomegranate fruit extract having a weight percentage of 0.2% in said composition; and (b) obtaining celery seed extract, wherein pomegranate

fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4-4:1.
[0080] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; (c) obtaining carriers, diluents, and excipients; and (d) contacting pomegranate fruit extract and celery seed extract, wherein said composition comprises: (a) pomegranate fruit extract; and (b) obtaining celery seed extract having a weight percentage of 0.8% in said composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0081] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit; (b) obtaining celery seed extract; (c) obtaining carriers, diluents, and excipients; and (d) contacting pomegranate fruit extract, celery seed extract and carriers, diluents, and excipients, wherein said composition comprises: (a) pomegranate fruit extract having a weight percentage of 0.6% in said composition; and (b) obtaining celery seed extract having a weight percentage of 0.4% in said composition, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1. [0082] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; (c) obtaining carriers, diluents, and excipients; and (d) contacting pomegranate fruit extract, celery seed extract and carriers, diluents, and excipients, wherein said composition comprises: (a) pomegranate fruit extract having a weight percentage of 0.6% in said composition; and (b) celery seed extract, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0083] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract having a weight percentage of 0.4%; (c) obtaining

carriers, diluents, and excipients; and (d) contacting pomegranate fruit extract, celery seed extract and carriers, diluents, and excipients, wherein said composition comprises: (a) pomegranate fruit extract having a weight percentage of 0.6% in said composition; and (b) celery seed extract, wherein, pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0084] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit; (b) obtaining celery seed extract; (c) obtaining carriers, diluents, and excipients; and (d) contacting pomegranate fruit extract, celery seed extract and (c) carriers, (d) diluents, and (e) excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0085] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; (c) obtaining carriers, diluents, and excipients; and (d) contacting pomegranate fruit extract, celery seed extract carriers, diluents, and excipients; and (d) excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0086] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (a) obtaining pomegranate fruit extract; (b) obtaining celery seed extract; (c) obtaining carriers, diluents, and excipients; and (d) contacting pomegranate fruit extract, celery seed extract and carriers, diluents, and excipients, wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4- 4:1.
[0087] Although the subject matter has been described with reference to specific embodiments, this description is not meant to be construed in a limiting sense. Various modifications of the disclosed embodiments, as well as alternate embodiments of the subject matter, will become apparent to persons skilled in the art upon reference to the description of the subject matter. It is therefore contemplated

that such modifications can be made without departing from the spirit or scope of the present subject matter as defined.
EXAMPLES
[0088] The disclosure will now be illustrated with working examples, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may vary.
Example 1
Material and Methods
[0089] Preparation of extracts: The commercially available pomegranate fruit extract was procured from Vidya Herbs, Bangalore. A stock solution pomegranate fruit extract powder was prepared by dissolving 25 mg/ml of solvent, preferably dimethyl sulfoxide (DMSO - 100%). A 50 - 250 µg/200 µL of pomegranate fruit extract was prepared by dissolving 2.0 µL - 10 µL of stock solution in phosphate buffer. Pomegranate fruit extract was standardized to 10% of polyphenols by TLC. The polyphenols are present in the range of 10% to 80% in this extract.
[0090] A stock solution of celery seed extract was prepared using commercially available celery seed extract from Naturex, France. A stock solution celery seed extract powder was prepared by dissolving 25 mg/ml of solvent, preferably dimethyl sulfoxide (DMSO - 100%). A 50 - 250 µg/200 µL of celery seed extract was prepared by dissolving 2.0 µL - 10 µL of stock solution in phosphate buffer. Celery seed extract was standardized to 8% of aglycone flavones by TLC. Aglycone flavones are present in in the range of 8% to 90% in this extract.

[0091] Preparation of stock solutions: 0.1M phosphate buffer, pH 7 was prepared by adding 30.75mL of 1M K2HPO4 and 19.25mL of 1M KH2PO4 and dilute to 500mL with distilled water and stored at room temperature.
[0092] The substrate 4-nitrophenyl α-D-glucopyranoside (Sigma-N1377) 10mM stock solution was prepared by dissolving 12.05mg of substrate in 4ml of assay buffer and stored at -20 °C.
[0093] Alpha-glucosidase enzyme solution was prepared from intestinal acetone powder from rat (Sigma-I1630). 200 mg of intestinal acetone powder was dissolved in 4 ml of ice cold phosphate buffer and sonicated for 15 mins at 4 °C. After vigorous vortex suspension was centrifuged at 10,000g at 4 °C for 30 min the resulting supernatant was used for the assay.
[0094] Alpha glucosidase activity assay: The principal behind the assay is that the alpha-glucosidase enzyme acts on the substrate p-nitrophenol conjugated and hydrolyses the α (1, 4) glucosidic linkage in the substrate to release free p-nitrophenol, which is a yellow coloured compound. The amount of p-nitrophenol thus released is estimated colorimetrically at 410nm.
[0095] The assay was carried out in a final reaction volume of 200 μL. A reaction mixture containing 164 μL of phosphate buffer and 100% activity wells and 154 μl of phosphate buffer in the ingredient blank and ingredient activity wells. 20 μl of substrate was added to all the wells. 10 μl ingredients were added to the ingredient blank and ingredient activity wells. Then as a control, 16 μl heat inactive enzyme was added in the blank and ingredient blank wells. Finally, 16 μl active enzyme was added to 100% activity and ingredient activity wells. The reaction mixtures were mixed thoroughly and the readings were taken at 37°C and 410 nm using kinetic loop for 30 minutes at the interval of every 1minute.
Example 2
Effect of pomegranate fruit extract and celery extract on alpha glucosidase
activity

[0096] In order to determine if the individual actives of pomegranate fruit extract and celery seed extract demonstrated any effect in the inhibiting alpha glucosidase activity, the ingredients were tested at different concentrations in isolation using the assay described above.
[0097] As is seen from the data (Figure 1), both pomegranate fruit extract and celery seed extract are able to inhibit the activity of the enzyme. In both cases, the percentage inhibition increases with increasing concentrations of the two actives. At a maximum concentration of 250 µl/200 µl (1.25 mg/ml) for each, pomegranate fruit extract shows a percentage inhibition of approximately 30% and celery seed extract shows a percentage inhibition of approximately 25%.
[0098] Thus, from this data it appears that both the actives under study- pomegranate fruit extract and celery seed extract, demonstrate the ability to repress alpha glucosidase enzyme activity.
Example 3
Effect of the combination of pomegranate fruit extract and celery seed extract
on alpha glucosidase activity
[0099] Following the observation that both the ingredients under study are able to
inhibit alpha glucosidase activity, combinations of pomegranate fruit extract and
celery seed extract were made and then tested for inhibitory activity against alpha
glucosidase, as listed in Table 1.

[00100] Unexpectedly, combinations of pomegranate fruit extract and celery
seed extract demonstrated distinct synergism in inhibiting the activity of alpha glucosidase. The w/w range of 1:4 to 4:1 ratio of pomegranate fruit extract to celery seed extract exhibited higher percentage inhibition than the actives in isolation. In isolation, pomegranate fruit extract and celery seed extract had percentage inhibition of 31.24% and 28.18%, respectively (Figure 1). On the other hand, as seen in Figure 2, the combination of the two extracts, particularly at an 80:20, 60:40 and 20:80 ratio of pomegranate fruit extract to celery seed extract showed percentage inhibition at 40.97%, 42.34% and 41.78%, respectively. The combinatorial effect of the actives, as compared to the effect of the actives in isolation, are much higher than what could be expected from a mere admixture of the two, and thus, distinct synergism is apparent in the combination of pomegranate fruit extract and celery seed extract.
[00101] Overall, the present disclosure provides a composition of pomegranate
fruit extract and celery seed extract, which exhibits a surprising and unexpected synergistic effect on alpha glucosidase activity and thus the management of metabolic diseases. This apparent synergism of the said combination is not readily apparent at all concentration combinations (as discussed previously). Further, the identification

of this novel combination for the management of metabolic and lifestyle-related diseases cannot be arrived at without undue experimentation, and is thus not obvious to a person skilled in the art.

I/We Claim:
1. A composition comprising:
(a) pomegranate fruit extract; and
(b) celery seed extract,
wherein pomegranate fruit extract to celery seed extract w/w ratio in said composition is in the range of 1:4-4:1.
2. The composition as claimed in claim 1 comprising:
(a) pomegranate fruit extract having a weight percentage in the range of 0.2%-0.8% in said composition; and
(b) celery seed extract having a weight percentage in the range of 0.2%- 0.8% in said composition.

3. The composition as claimed in claim 1, wherein pomegranate fruit extract weight percentage in said composition is 0.2% and celery seed extract weight percentage in said composition is 0.8%.
4. The composition as claimed in claim 1, wherein pomegranate fruit extract weight percentage in said composition is 0.6% and celery seed extract weight percentage in said composition is 0.4%.
5. The composition as claimed in claim 1, wherein pomegranate fruit extract weight percentage in said composition is 0.8% and celery seed extract weight percentage in said composition is 0.2%.
6. The composition as claimed in any of the claims 1-5, further comprising carriers, diluents, and excipients.
7. A method of preparing a composition as claimed in any of the claims 1-5, said method comprising:

(a) obtaining pomegranate fruit extract;
(b) obtaining celery seed extract; and
(c) contacting pomegranate fruit extract and celery seed extract to obtain said composition.

8. A method of preparing a composition as claimed in any of the claims 1-6, said
method comprising:
(a) obtaining pomegranate fruit extract;
(b) obtaining celery seed extract;
(c) obtaining carriers, diluents, and excipients; and
(d) contacting pomegranate fruit extract, celery seed extract, carriers, diluents, and excipients to obtain said composition.
9. The composition as claimed in any of claims 1-6, wherein said composition treats
metabolic diseases and complications arising from said diseases, through inhibition of
alpha-glucosidase activity.