Art
[1]
The present invention relates to animal feed compositions comprising this composition, and for feed additives.
BACKGROUND
[2]
Methionine is used as a kind of essential amino acid in the living body, animal feeds, food additives, materials for synthesis, such as the pharmaceutical and suaekje medicine. Methionine may be produced by chemical synthesis and biological synthesis.
[3]
Chemical synthesis is mainly 5- (β- methyl-mercaptoethyl) - to the high-stage through the reaction for hydrolysis of hydantoin (5- (β-methylmercapto ethyl) -hydantoin) produce D, L- methionine.
[4]
Examples of biological composite, U.S. Patent US7745195B2 discloses a system tachioh non synthase was added to a variation on (cystathionine synthase) microorganisms without the use of cysteine directly H 2 S or CH 3 A method of producing a homocysteine or methionine by using the SH directly It discloses a. In addition, the Republic of Korea Patent No. 10-0905381 discloses a two-stage method of producing L- methionine via the enzyme conversion reaction it as a substrate and then producing L- methionine precursor by microbial fermentation.
[5]
Conventional chemically produced methionine has been produced in the form a D, L- methionine mixed in order to obtain only the L- methionine requires expensive additional purification reaction. On the other hand, methionine is produced directly from a microorganism as a biological synthesis is the advantage of L-form, but this yields a very small amount, as well as difficult to produce a large-scale fermentation, it is difficult to obtain a fermented product having a uniform state. Therefore, in the conventional low feed additives it could be included as a main component L- methionine.
[6]
[Prior art document]
[7]
[Patent Document]
[8]
(Patent Document 1), US 7745195B2 (published on 06.29.2010)
[9]
(Patent Document 2) KR 10-0905381B1 (gonggoil 06.30.2009)
Detailed Description of the Invention
SUMMARY
[10]
The present inventors obtained the result of extensive studies to overcome the problems as described above, L- methionine through after the enzymatic conversion reaction produce L- methionine precursor by microbial fermentation in large quantities. Since, for this it is possible to provide a high-L- methionine content of the composition uniform quality throughout the purification process development and feed additives containing these compositions is thereby completing the present invention discovered show an excellent effect in animal .
[11]
Accordingly, it is an object of the invention to provide a composition for the L- methionine content higher feed additives.
[12]
The invention further object is to provide an animal feed composition comprising the composition.
Problem solving means
[13]
In order to achieve the above object, the present invention provides for the feed additive composition comprising the L- tyrosine of 60 to 99.90% by weight of L- methionine, 0.05 to 5% by weight of L- phenylalanine, and 0.01 to 3% by weight of.
[14]
The present invention also provides an animal feed composition comprising the composition.
Effects of the Invention
[15]
The present invention is the content of L- methionine which may be mass produced by a L- methionine through the production thereof, and enzyme conversion of L- methionine precursor by microbial fermentation and crystallized, improve the feeding of the animals compared to conventional feed additive this high feed additive composition and is useful for providing animal feed composition comprising the same.
Mode for the Invention
[16]
Hereinafter, the present invention will be described in detail.
[17]
As a first aspect of the invention, the invention provides a composition for the L- methionine content higher feed additives.
[18]
L- methionine content of the composition is high is to include an L- methionine content is increased, it is possible to specifically include L- methionine and L- phenylalanine, and L- tyrosine. Additionally, the L- methionine content may include one or more additional nutrients other than the L- methionine, L- phenylalanine, and L- tyrosine in a high composition. Here, the nutrients may be an other amino acid, acetate ion, except for L- methionine, L- phenylalanine, and L- tyrosine, and the like.
[19]
The compositions in the present invention can be incorporated without producing method is capable of including L- methionine in an excess limited. For example, according to the a two-step process indicated in the existing patent L- methionine production method comprising after producing L- methionine, but may be produced through a crystallization process, and the like.
[20]
Specifically, L- methionine production method consists of the two-step process Republic of Korea Patent No. 10-0905381 call, the Republic of Korea Patent No. 10-0905381 call, the Republic of Korea Patent No. 10-1136289 call, the Republic of Korea Patent No. 10-1117012 No. , is disclosed in Republic of Korea Patent No. 10-1200179 call, the Republic of Korea Patent No. 10-1250651 and No. or the like, this patent is that the entire contents incorporated by reference.
[21]
More specifically, the step of L- methionine production method consists of the two-step process to produce L- methionine precursor by culturing the L- methionine precursor producing strain in a culture medium as a first step; And as step 2 was added to the hwangwon L- methionine precursor produced in the above step by step consists of producing L- methionine by enzyme reaction.
[22]
First, L- methionine precursor may be produced by culturing a microorganism or a recombinant mutant microorganism capable of producing it in an appropriate medium. Specifically, L- methionine precursor acyl homoserine O- (O-acyl homoserine), for example, O- acetyl homoserine (O-Acetyl homoserine), O- succinyl-homoserine (O-succinyl homoserine), propynyl O'Neill homoserine (Propionyl homoserine), acetoacetyl-homoserine (acetoacetyl homoserine), one-homoserine (Coumaroyl homoserine), malonyl-homoserine (malonyl homoserine), hydroxymethyl-glutaryl-homoserine (Hydroxymethylglutaryl homoserine) in Kumar, P. Merrill include, homoserine (Pimelylhomoserine), but is not limited to such. Than may be specifically, O- acetyl homoserine (O-Acetyl homoserine) or O- succinyl-homoserine (O-succinyl homoserine), can be more specifically, O- acetyl homoserine.
[23]
Culture process of L- methionine precursor-producing strain can be performed according to a suitable culture medium and culture conditions known in the art. This culturing process may be used to easily adjusted according to the strains selected by those skilled in the art. Examples of the culturing method, but includes the batch type, continuous type and fed-batch culture, but is not limited to this. These various culture methods, for example, are disclosed in literature ( "Biochemical Engineering" by James M. Lee, Prentice-Hall International Editions, pp 138-176).
[24]
The medium used to culture should satisfy the requirements of the particular strain, as appropriate. Media of various microorganisms, for example, are disclosed in literature ( "Manual of Methods for General Bacteriology" by the American Society for Bacteriology, Washington DC, USA, 1981). The medium may include a variety of carbon sources, nitrogen sources and trace element ingredients. Carbon source is carbohydrates such as glucose (glucose), lactose (lactose), sucrose (sucrose), milk sugar, fruit sugar (fructose), maltose (maltose), starch (starch) and cellulose (cellulose); Such as soybean oil (soybean oil), sunflower oil (sunflower oil), castor oil, Weber oil (castor oil) and coconut oil (coconut oil) fat; Palmitic acid (palmitic acid), stearic acid (stearic acid) and linoleic acid (linoleic acid) acids such as fatty acids; It may include glycerol (glycerol), and ethanol (ethanol) an organic acid (organic acid) such as an alcohol and acetic acid (acetic acid), such as, but not limited to this. These carbon sources may be used alone or in combination. The nitrogen source is peptone (peptone), yeast extract (yeast extract), gravy (gravy), malt extract, organic nitrogen sources, and elements, such as (malt extract), corn leachate (corn steep liquor (CSL)) and soy flour (bean flour) ( urea), ammonium sulfate (ammonium sulfate), ammonium chloride (chloride), Ammonium phosphate (phosphate), ammonium carbonate may include an inorganic nitrogen source such as (carbonate) and ammonium nitrate (nitrate), but is not limited to this. These nitrogen sources may be used alone or in combination. The culture medium, but may include further potassium dihydrogen phosphate (potassium dihydrogen phosphate), potassium hydrogen phosphate (dipotassium hydrogen phosphate) and the corresponding sodium include salts (sodium-containing salts) of a phosphoric acid source, but not limited to, . In addition, the culture medium, but can comprise a metal, such as magnesium sulfate (magnesium sulfate) or iron sulfate (iron sulfate), but is not limited to such. In addition, although such as amino acids, vitamins and proper precursors it can be added, and the like. These media or precursors may be added batchwise or continuously to the culture. Medium, but it may include a metal, such as magnesium sulfate (magnesium sulfate) or iron sulfate (iron sulfate), but is not limited to such. In addition, although such as amino acids, vitamins and proper precursors it can be added, and the like. These media or precursors may be added batchwise or continuously to the culture. Medium, but it may include a metal, such as magnesium sulfate (magnesium sulfate) or iron sulfate (iron sulfate), but is not limited to such. In addition, although such as amino acids, vitamins and proper precursors it can be added, and the like. These media or precursors may be added batchwise or continuously to the culture.
[25]
In addition, compounds such as ammonium hydroxide, potassium hydroxide, ammonia, phosphoric acid and sulfuric acid is added in a suitable manner to the culture in the culture can control the pH of water culture. Further, it is possible to suppress the air bubbles generated by using antifoaming agents such as fatty acid polyglycol ester in the culture. Further, in order to maintain the culture of water aerobic conditions, oxygen or oxygen into the culture-containing gas can be injected (for example, air).
[26]
Incubation temperature of water may be usually 20 to 45 ℃, preferably from 25 to 40 ℃. The incubation period may continue until it is obtained the production of the desired L- methionine precursor, preferably from 10 to 160 hours, more preferably from 15 to 80 hours.
[27]
Next, through the L- methionine L- methionine precursor producing strain produced by the precursor and hwangwon a converting enzyme or enzyme conversion reaction using the strain containing the conversion enzyme using as a substrate can produce L- methionine and organic acid. The converting enzyme may be a hwangwon transferase, specifically cis tachioh non synthetase (Cystathionine synthase) or O- succinyl homoserine kinase deurilra sulfinyl (O-succinylhomoserine sulfhydrylase) or O- acetyl homoserine kinase deurilra sulfinyl (O- enzyme, and more specifically having a acetylhomoserine sulfhydrylase) activity may be an enzyme having the O- acetyl homoserine sulfinyl deurilra dehydratase activity. The hwangwon is of a material which supplies a sulfur atom, it may be methyl mercaptan or a salt thereof, specifically, it can more specifically be used to methylmercaptan.
[28]
In one embodiment, the O- succinyl-homoserine (O-succinyl homoserine) or O- using hwangwon such as acetyl-homoserine and methyl mercaptan to the substrate system tachioh non-gamma synthase (cystathionine gamma synthase accumulated in microbial fermentation ) or O- succinyl homoserine kinase deurilra sulfinyl (O-succinylhomoserine by the enzyme reaction, such as sulfhydrylase) or O- acetyl homoserine kinase deurilra sulfinyl (O-acetylhomoserine sulfhydrylase) can produce L- methionine, specifically O - it can produce L- methionine and acetate using O- acetyl homoserine sulfinyl deurilra enzyme using as a substrate, acetyl-homoserine and methylmercaptan.
[29]
Sequence of the gene encoding the enzyme having the activity can be easily obtained from databases such as the US Center for Biotechnology Information (NCBI) and the DNA Data Bank of Japan (KEGG).
[30]
An L- methionine production in the same manner as described above can be obtained by separation via crystallization, it can be purified, high-L- methionine content of the present invention by this composition.
[31]
More specifically, the crystallization method is L- methionine
[32]
a) after separating the crystallized methionine was concentrated methionine reaction solution and recovering the mother liquor; And
[33]
b) separating the produced methionine granules using a crystal as a seed, and by drying the resulting granular powder solidifying step
[34]
It may contain.
[35]
According to a further variant crystallization method, a) the separated mother liquor in step is
[36]
a-1) are concentrated and crystallized again, b) or the mixture is washed with methionine separated from the crystals and dried,
[37]
a-2) is dissolved and then re-concentrated and the crystallization is added to the other methionine reaction solution.
[38]
Additionally, the crystallization method may include the step of adjusting the methionine reaction solution prior to said step a) to pH 4.0 ~ 5.5, may also include the step of filtration after the pH adjustment step, methionine reaction solution with activated charcoal .
[39]
In one implementation, the high-L- methionine content of the composition according to the invention In the sulfuric acid concentration before or concentrated to the concentration of methionine in the L- methionine reaction solution obtained by the reaction conversion of the enzyme to be 150 ~ 200 g / L then to a fair and concentrated to pH 4.0 ~ 5.5, with a crystal separator to separate the crystal mother liquid (mother liquid, ML) a number of times, and using the separated methionine crystal as a seed (seed) spray dried the mother liquor from the granulator and it is obtained by pulverization. The content of the so obtained composition L- methionine L- methionine may be from about 60 to the range of 70% by weight relative to the total weight of the composition.
[40]
In another implementation, L- methionine compositions according to the invention pH4 by introducing the sulfuric acid concentration before or concentrated to the concentration of methionine in the L- methionine reaction solution obtained by the reaction conversion of the enzyme to be 150 ~ 200 g / L. after titration, and concentrate 0 to 5.5, and concentrated using a crystal separator until the first separating the crystals drive to recover the mother liquor, and become re-methionine concentration of 150 ~ 200 g / L of the separated mother liquor of the secondary crystals obtained, mixing the thus obtained primary crystals and secondary crystals, washing and drying is obtained by this. The content of the so obtained composition L- methionine L- methionine may be a range of about 80 to 95% by weight relative to the total weight of the composition.
[41]
In another implementation, L- methionine compositions according to the invention was added to the activated carbon of 0.5 to 2% of the titration, the total amount of methionine to pH 4.0 ~ 5.5 by introducing sulfuric acid to L- methionine reaction solution obtained by the reaction conversion of the enzyme and then a solution for 1-2 hours at 50 ℃ by filtration using a concentrated, crystal separator until remove the activated carbon and the impurities, and the art to filtrate the concentration of methionine to be 150 ~ 200 g / L of one crystal drive recovered. By concentration of the separated mother liquor until the concentration of methionine again be 150 ~ 200 g / L obtained secondary crystals to dissolve the secondary crystals thus obtained is obtained by using the reclosing L- methionine in the reaction mixture. The content of the so obtained composition L- methionine L- methionine may be from about 95 to the range of 99.90% by weight relative to the total weight of the composition.
[42]
The separation, the amount of L- methionine in a composition of the invention obtained by the purification may be based on the total weight of the composition, from 60 to 99.90% by weight. In one embodiment, the composition may comprise a L- methionine of about 60 to 70% by weight. In another embodiment, the composition may comprise a L- methionine of about 80 to 95% by weight. In yet another embodiment, the composition may comprise a L- methionine of about 95 to 99.90% by weight.
[43]
Specifically, the L- methionine content in accordance with the purification method for high feed additive composition is 60 to 99.90% by weight of 0.05 to 5% by weight with L- methionine L- phenylalanine and 0.01 to 3% by weight of L- It may contain tyrosine.
[44]
The L- methionine content of the feed additive composition for high may comprise other amino acids in the range of 0.01 to 13% by weight, except for the L- methionine, L- phenylalanine, and L- tyrosine, in which the amino acid is glutamate, homoserine or It may be at least one selected from the group consisting of O- acetyl-homoserine.
[45]
The amount of the glutamate may be from 0.01 to 11% by weight, based on the total weight of the composition.
[46]
The amount of the homoserine may be 0.01 to 1% by weight, based on the total weight of the composition.
[47]
The content of O- acetyl-homoserine can be 0.01 to 1% by weight, based on the total weight of the composition.
[48]
In addition, the L- methionine content is high the composition may comprise additional ion of 0.01 to 20% by weight.
[49]
In addition, the L- methionine content is high composition may include the acetate weight percent in the range from 0 to greater than 2 added.
[50]
Feed additive composition of the present invention is L- methionine is not however possible to obtain a high separation, L- methionine content of the tablet obtained by the crystallization composition, limited as described above. Feed additive composition of the present invention although not described above may further comprise any other nutrient components in the range in which those skilled in the art to expect.
[51]
[52]
As a second aspect of the invention, the present invention provides an animal feed composition comprising the composition for the addition of the feed.
[53]
The feed additive composition is for those skilled in the art to include the expected range, it may be adjusted to an appropriate level depending on the type of animal, age and conditions used. Specifically, the feed additive may be included in the range of 0.01 to 0.5% by weight.
[54]
L- methionine feed composition for a high content of the addition of the present invention may be incorporated into a commercially conventional animal feed composition, for example, be fed cattle, pigs, sheep, poultry or the like. Feed composition of the present invention is added for this purpose may be mixed with conventional animal feed composition may be processed into a tableted form, if necessary.
[55]
Conventional animal feed constituents, for example, corn, and barley, oats, soybean, fish meal, bran, soybean oil, mineral, trace elements, amino acids and vitamins.
[56]
And in more details below the present invention through the embodiments. It is, however, not intended that the scope of the present invention are for the purpose of illustrating the invention by way of example only to the examples.
[57]
[58]

[59]
Example 1: Fermentation of L- methionine precursor producing strain
[60]
In this embodiment, the fermentation culture was carried out to produce L- methionine precursor.
[61]
A 5L fermentor culture in order to mass-produce the methionine precursor (O- acetyl homoserine) using O- acetyl homoserine-producing strain E. coli KCCM-10568 (Republic of Korea Patent No. 10-0905381 No.) was performed as a methionine precursor producing strain . This strain was inoculated on LB medium containing antibiotics, the plate is incubated overnight at 31 ℃. Then, was inoculated in 10 ml LB medium containing single colonies antibiotics 5 hours incubation at 31 ℃, and the 100-fold diluted in 1000ml Erlenmeyer flask containing the re 200ml methionine precursor seed (Seed medium) 31 ℃, 200 rpm from 3-10 hours fed-batch culture (Fed batch) it was incubated with 50-100 hours fermentation was inoculated in 5L fermenter and then incubated. The composition of the main culture fermentation medium shown in Table 1 below.
[62]
[63]
[Table 1] methionine precursor production fermenter medium composition
Furtherance Seed medium (seed media) State media (Main media) Feed medium (Feed media)
Glucose(g/L) 10.1 40 600
MgSO4·7H20(g/L) 0.5 4.2
Yeast extract(g/L) 10 3.2
Kh 2 the PO 4 3 3 8
Ammonium sulfate(g/L) 6.3
NH4Cl(g/L) 1
NaCl(g/L) 0.5
Na2HPO4·12H2O(g/L) 5.07
DL-Methionine(g/L) 0.5 0.5
L-Isoleucine(g/L) 0.05 0.5 0.5
L-Threonine(g/L) 0.5 0.5

[64]
[65]
Example 2: Methionine conversion
[66]
By filtering the fermentation broth produced in Example 1 by using membrane filtration (membrane filtration), it was isolated O- acetyl homoserine culture broth and cells. As a liquid, i.e. the remaining liquid by separating the cell transmission (permeate) pass by 0.1㎛ of the film, and the cell sludge (cell sludge) was as reserves (retentate).
[67]
The O- acetyl homoserine remaining by the addition of deionized water (deionized water) was collected in the retention material.
[68]
The permeate O- acetyl homoserine kinase deurilra sulfinyl (O-acetylhomoserine sulfhydrylase) of O- acetylation using the strain containing the enzyme or the enzyme which is active methoxy tipmeo mercaptan and L- methionine-converting enzyme homoserine kinase or a sulfinyl deurilra Rhodobacter spheroid (Rhodobacter sphaeroides) was performed resulting O- acetyl homoserine sulfide Kerala dehydratase (O-acetylhomoserine sulfhydrylase) (Republic of Korea Patent No. 10-1250651 No.) enzyme conversion reaction by the addition of.
[69]
By measuring the concentration of residual O- acetyl-homoserine in the reaction and do the enzyme conversion reaction for 6 hours by supplying the methylmercaptan was complete the reaction when it is not determined that the concentration of O- acetyl-homoserine.
[70]
[71]
Example 3: The process of crystallization of L- methionine
[72]
In order to obtain a high content of L- methionine composition, and crystallizing methionine The reaction solution prepared in Example 2. By crystallization method according to the present embodiment it was able to obtain a composition containing a methionine at least 60.00% by weight to up to 99.90% by weight.
[73]
(1) A method of crystallizing
[74]
Example 2 was carried out, the reaction mixture was concentrated until the concentration of methionine in the methionine reaction solution is 150 ~ 200g / L in a, to thereby recover the crystal by using a separator separating the crystal mother liquid (mother liquid, ML). Using the separated methionine as a seed crystal (seed), and dried while spraying a stock solution in a granulator to obtain a powdered composition methionine.
[75]
In this way the resulting methionine to the composition shown in Table 2 below.
[76]
TABLE 2
Furtherance (%)
L-Methionine 60~70
Acetate 0~1
Ion 13~19
Phenylalanine 0.05~4.5
Tyrosine 0.02~2.5
Glutamate 0.5~11
Homoserine 0.05~1
O-Acetylhomoserine 0.1~1

[77]
[78]
(2) B method of crystallizing
[79]
Example 2 concentration of the reaction solution at methionine or injecting the sulfuric acid and concentrated after appropriate to pH 4.0 ~ 5.5. Methionine was a concentration of the reaction mixture was concentrated until 150 ~ 200g / L, were determined using a separator separating the first crystal mother liquor, and recovering. By concentration of the primary crystals are separated mother liquor until the concentration of methionine again be 150 ~ 200g / L to obtain a second crystal. It was washed with a mixture of primary crystals and the secondary crystals made by this dry powdered methionine composition.
[80]
Thus Below is the composition of the resulting methionine in Table 3.
[81]
[82]
TABLE 3
Furtherance (%)
L-Methionine 80~95
Acetate 0~1
Ion 0.5~5.5
Phenylalanine 0.05~4
Tyrosine 0.01~2
Glutamate 0.05~5.5
Homoserine 0.05~1
O-Acetylhomoserine 0.1~1

[83]
[84]
(3) C Crystallization Method
[85]
In the embodiment of sulfuric acid methionine reaction solution obtained in Example 2 it was adjusted to pH 4.0 ~ 5.5. Added to the reaction solution from 0.5 to 2% by weight of activated carbon of methionine, the total amount (active carbon) were mixed for 1 ~ 2hr at 50 ℃ was filtered to remove the activated carbon and impurities. The filtrate was concentrated to the concentration of methionine until the 150 ~ 200g / L, with a crystal separator to obtain the crystals. The separated mother liquor and the crystals were recovered was used to back was concentrated to recover the secondary crystals with the secondary crystals obtained are re-introduced to the reaction solution L- methionine titration to pH 4.0 ~ 5.5 and dissolved, repeat the above process.
[86]
Thus Below is the composition of the resulting methionine in Table 4.
[87]
TABLE 4
Furtherance (%)
L-Methionine 95.00~99.90
Acetate 0~1
Ion 0.01~0.2
Phenylalanine 0.05~1
Tyrosine 0.01~1
Glutamate 0.01~1
Homoserine 0.01~0.8
O-Acetylhomoserine 0.0~0.5

[88]
[89]
Example 4: Confirmation of the composition efficacy methionine
[90]
The experimental design to determine the effect of methionine composition obtained in Example 3 was conducted. Was used in this embodiment, in Example 3 (hereinafter referred to, L-Met) is a high composition L- methionine content obtained through C crystallization method, a composition containing the D, L- methionine as a control (where D, L -methionine was used as a chemical method and a purity of greater than 99% of production, the following, D, it referred to as L-Met).
[91]
(1) The efficacy of the composition L- methionine at 25-week-old chickens weaning
[92]
To determine the effect of the eggs, were each treated with L-Met, and D, L-Met in the laying hen.
[93]
Experimental Design
[94]
- weaning chickens taeeonanji 25 weeks (25wk of age, laying hens)
[95]
-Based diet (basal diet, BD), respectively 0.1% by weight of L-Met or D, L-Met, the addition in an amount of 0.2% by weight
[96]
The base diet composition
[97]
Table 5 The composition of the basic diet
% 　
Corn 50
Wheat 7
Wheat Bran 3
Soybean meal 26
Others 14

[98]
Table 6 Results
unit % Egg production rate(%) Egg weight(g/egg) Egg mass(g/day/bird)
BD 100.0 100.0 100.0
L-Met 0.1% 103.6 104.1 107.8
0.2% 104.9 104.4 109.7
DL-Met 0.1% 101.9 102.7 104.6
0.2% 104.6 102.7 107.4
DL-Met-Met increase compared to L 0.1% 101.6 101.3 103.1
0.2% 100.3 101.7 102.1

[99]
[100]
As can be seen from the above results, it was confirmed that bringing a good effect on the eggs in both the control of D, L-Met-treated group and the L-Met-treated group compared with the basic diet. In comparison the L-Met-treated group and D, L-Met-treated group, the weight of the L-Met-treated group producing ability and Al eggs in was improved approximately 1% and a thickness of Al is shown from about 2-3% increase gave.
[101]
The D, check bringing a positive effect on the scattering coefficient was the addition of L-Met than L-Met was added.
[102]
(2) the efficacy of the composition of L- methionine in piglets
[103]
To confirm the efficacy of the composition of L- methionine in piglets, L-Met, and D, L-Met, respectively after treatment the body weight gain per day were compared.
[104]
Experimental Design
[105]
- piglets (piglet)
[106]
-Based diet (basal diet, BD) each 0.05% by weight of L-Met or D, L-Met, the addition in an amount of 0.11% by weight
[107]
Based dietary composition
[108]
Table 7
% 　
Corn 60.7
SBM 3.0
Plasma protein 11.0
Whey dried 20.0
Grease 1.0
Sand
Corn starch 0.5
others 3.8

[109]
[110]
result
[111]
Table 8
ADG (kg/d) Basal diet Added DL-Met, % Added L-Met, %
0.05 0.11 0.05 0.11
0-7 day 100.0 151.6 159.1 171.1 201.9
14-21day 100.0 120.6 126.9 125.3 132.2

[112]
* ADG: average daily weight gain (Average daily gain)
[113]
[114]
As can be seen from the result, little day comparison of the weight gain based diet compared to D, L-Met treatment group of pigs was improved about 60% up to, L-Met-treatment group was confirmed the improved up to 100%. And, compared to D, L-Met-L-treated group compared to the group treated Met, L-Met treatment group was confirmed to acquire a high growth rate of approximately 5-40% compared to the D, L-Met-treated group.
[115]
(3) Effect of the composition of L- methionine in broilers
[116]
In order to confirm the efficacy of the composition of L- methionine in broilers, and then the L-Met, and D, L-Met, each treatment was compared to body weight gain efficiency (Gain: Feed ratio, G: F ratio).
[117]
Experimental Design
[118]
- taeeonanji 1-day-old broiler chickens (1d of age, ross308)
[119]
-Based expression added in an amount of 0.1% by weight of the L-Met or D, L-Met (basal diet, BD), respectively, 0.2% by weight, 0.3% by weight
[120]
Based dietary composition
[121]
Table 9
% 　
Yellow corn 59
Soybean meal 34
Others 7

[122]
result
[123]
[124]
[Table 10]
Gain:feed BD(%) Added DL-Met, % Added L-Met, % Increased amount of DL-Met than L-Met (%)
0.1 0.2 0.3 0.1 0.2 0.3 0.1 0.2 0.3
0 ~7 day 100.0 102.0 101.2 102.0 102.8 106.1 106.1 100.8 104.8 104.0
7~14 day 100.0 111.9 112.7 114.1 113.9 114.8 115.8 101.8 101.9 101.5
14~21 day 100.0 116.1 118.1 118.6 116.7 122.1 124.7 100.6 103.4 105.2
0~21day 100.0 112.8 113.9 114.7 113.9 117.4 119.2 101.0 103.1 103.9

[125]
[126]
As can be seen from the results, there was a body weight gain efficiency in all groups the L-Met or DL-Met is added to the base diet can check the jotahjim. Compared to L-Met-treated group and D, L-Met-treated group, it was confirmed that L-Met treatment group with a high body weight gain efficiency of up to 0.5% ~ 5% by the end of the early testing, the end of the test in point L-Met in the treated group was found that showing high body weight gain efficiency of about 1-4%.

Claims
[Claim 1]
60 to 99.90% by weight of L- methionine, L- tyrosine containing from 0.05 to 5% by weight of L- phenylalanine, and 0.01 to 3% by weight, feed additive composition.
[Claim 2]
The method of claim 1, wherein the composition is a composition comprising the other amino acids in the range of 0.01 to 13% by weight, except for the L- methionine, L- phenylalanine, and L- tyrosine in addition.
[Claim 3]
The method of claim 2 wherein the other amino acids comprising a glutamate, glutamate content of such a composition is from 0.01 to 11% by weight, based on the total weight of the composition.
[Claim 4]
The method of claim 2 wherein the other amino acids comprising a homoserine, homoserine content of such a composition is from 0.01 to 1% by weight, based on the total weight of the composition.
[Claim 5]
The method of claim 2, wherein said other amino acid is O- acetyl comprises homoserine, O- art The content of acetyl-homoserine is the composition 0.01 to 1% by weight, based on the total weight of the composition.
[Claim 6]
The method of claim 1, wherein the composition is a composition comprising an additional ion of 0.01 to 20% by weight.
[Claim 7]
The method of claim 1, wherein the composition is a composition containing more of the acetate weight% within a range from 0 to greater than 2.
[Claim 8]
Any one of claims 1 to animal feed compositions comprising either of a composition of claim 7 wherein.