CRYSTALLINE ROSUVASTATIN CALCIUM
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional
Application No. 60/845,584, filed September 18,2006. The contents of which are incorporated herein by reference.
FIELD OF THE INVENTION
[0002] The invention relates to a crystalline rosuvastatin calcium and a
process for the preparation thereof.
BACKGROUND OF THE INVENTION
[0003] Rosuvastatin (monocalcium bis (+) 7-[4-(4-fluorophenyl)-6-isopropyl-
2-(N-methyl-N-methylsulfonylarm^c^yrirmdm)-5-yl]-(3R,5S)-dihydroxy-(E)-6-heptenoate) is an HMG-CoA reductase inhibitor, developed by Shionogi for the once daily oral treatment of hyperlipidaemia (Ann Rep, Shionogi, 1996; Direct communications, Shionogi, 8 Feb 1999 & 25 Feb 2000). Rosuvastatin calcium is a superstatin, which c;JJ lower LDL-cholesterol and triglycerides more effectively than first generation statin drugs. Rosuvastatin calcium has the following chemical formula:
(Formula Removed)
Rosuvastatin calcium is marketed under the name CRESTOR for treatment of a rnammal*such as a human. According to the maker of CRESTOR, it is administered in. a daily dose of from about 5 mg to about 40 mg for LDL cholesterol reduction. For patients requiring less aggressive LDL-C reductions or who have pre-disposing factors for myopathy, a 5 mg dose is recommended, while 10 mg dose is
recommended for the average patient, 20 mg dose for patients with marked hypercholesterolemia and aggressive lipid targets (>190 mg/dL), and the 40 mg dose for patients who have not been responsive to lower doses.
[0004] Rosuvastatin calcium salt often precipitates as an amorphous material.
[0005] US 6,589,959 discloses a crystalline form of rosuvastatain calcium,
designated form A. WO 2005/023779 discloses a crystalline hydrated form of rosuvastatin calcium, designated form B5 and the dehydrated form, designated form B-l.
[0006] The present invention relates to the solid state physical properties of
rosuvastatin calcium. Solid state physical properties include, for example, the flowability of the milled solid. Flowability affects the ease with which the material is handled during processing into a pharmaceutical product. When particles of the powdered compound do not flow past each other easily, a fonnulation specialist must take that fact into account in developing a tablet or capsule formulation, which may necessitate the use of glidants such as colloidal silicon dioxide, talc, starch, or tribasic calcium phosphate.
[0007] Another important solid state property of a pharmaceutical compound
is its rate of dissolution in aqueous fluid. The rate of dissolution of an active
ingredient in a patient's stomach fluid can have therapeutic consequences since it
imposes an upper limit on the rate at which an oraUy-adrninistered active ingredient
can reach the patient's bloodstream. The rate of dissolution is also a consideration in
formulating syrups, elixirs and other liquid medicaments. The solid state form of a
compound may also affect its behavior on compaction and its storage stability.
[0008] These practical physical characteristics are influenced by the
conformation and orientation of molecules in the unit cell, which defines a particular polymorphic form of a substance. The polymorphic form may give rise to thermal behavior different from that of the amorphous material or another polymorphic form. Thermal behavior is measured in the laboratory by such techniques as capillary melting point, thermogravimetric analysis (TGA) and differential scanning calorimetric (DSC) and can be used to distinguish some polymorphic forms from others. A particular polymorphic form may also give rise to distinct spectroscopic properties that may be detectable by powder X-ray crystallography, solid state 13C NMR spectrometry, and infrared spectrometry.
[0009] One of the most important physical properties of a pharmaceutical
compound, which can form polymorphs or solvates, is its solubility in aqueous solution, particularly the solubility in gastric juices of a patient. Other important properties relate to the ease of processing the form into pharmaceutical dosages, as the tendency of a powdered or granulated form to flow and the surface properties that determine whether crystals of the form will adhere to each other when compacted into a tablet.
[00010] There is a need in the art for new polymorphic forms of rosuvastatin
calcium.
SUMMARY OF THE INVENTION
[00011] In one embodiment, the present invention provides a crystalline form
of rosuvastatin calcium characterized by an X-ray powder diffraction pattern having
peaks at about 4.7, 19.4 and 22.3° 29 ± 0.2° 26.
[00012] The invention also encompasses a process for preparing a crystalline
form of rosuvastatin calcium characterized by X-ray powder diffraction peaks at
about 4.7,19.4 and 22.3° 29 ± 0.2° 29.
[00013] In another embodiment, the invention provides a pharmaceutical
composition including the crystalline rosuvastatin calcium of the present invention, or
a pharmaceutically acceptable salt thereof, and at least one pharmaceutically
acceptable excipient.
[00014] In a further embodiment, the invention provides a process for
preparing a pharmaceutical formulation including combining crystalline rosuvastatin
calcium of the present invention, or a pharmaceutically acceptable salt thereof, with at
least one pharmaceutically acceptable excipient.
[00015] The invention further provides the use of crystalline rosuvastatin
calcium of the present invention, or a pharmaceutically acceptable salt thereof, for the
manufacture of a pharmaceutical composition.
[00016] The invention also provides a method of treatment including
administering a pharmaceutical composition containing the rosuvastatin calcium of
the present invention, or prepared from the rosuvastatin calcium of the present
invention, to a mammal in need thereof.
BRIEF DESCRIPTION OF THE FIGURES
[00017] Fig 1 illustrates a characteristic X-Ray Powder Diffractogram of
crystalline rosuvastatin calcium having X-ray powder diffraction peaks at about 4.7, 19.4 and 22.3° 29 ±0.2° 29.
[00018] Fig. 2 illustrates a characteristic DSC thermogram of crystalline
rosuvastatin calcium having X-ray powder diffraction peaks at about 4.7,19.4 and 22.3° 26 ±0.2° 29.
[00019] Fig. 3 illustrates a characteristic TGA spectrum of crystalline
rosuvastatin calcium having X-ray powder diffraction peaks at about 4.7,19.4 and 22.3° 26 ± 0.2° 26.
DETAILED DESCRIPTION OF THE INVENTION
[0002O] The discovery of new polymorphic forms of a pharmaceutically useful
compound provides a new opportumty to improve the performance characteristics of a pharmaceutical product. It enlarges the repertoire of materials that a formulation scientist has available for designing, for example, a pharmaceutical dosage form of a drug with a targeted release profile or other desired characteristic. The invention provides a new polymorphic form of rosuvastatin calcium.
{00021] The invention encompasses a crystalline form of rosuvastatin calcium
characterized by an X-ray powder diffraction pattern having peaks at about 4.7, 19.4 and 22.3° 26 ± 0.2° 26. The crystalline form may be further characterized by X-ray powder diffraction peaks at about 6.9,11.1, 15.7, 20.9 and 24.3° 26 ± 0.2° 29. The crystalline form may also be substantially identified by the PXRD pattern depicted in Figure 1.
[00022] The crystalline form of the invention may be further characterized by a
DSC thermogram with an endotherm at about 132°C and another broad endotherm at about 220°C to about 240°C. The crystalline form may also be substantially identified by the DSC curve depicted in Figure 2.
[00023] In addition, the crystalline form may be further characterized by a
TGA thermogram showing a weight loss of about of 3 to about 5 percent up to about 100°C. Moreover, the crystalline form may be substantially identified by the TGA
curve depicted in Figure 3. According to Karl-Fisher analysis, the water content of
the crystalline rosuvastatin calcium is the same as measured by TGA.
[00024] Preferably, the crystalline rosuvastatin calcium is polymorphically
pure, i.e., contains no more than about 30 % of rosuvastatin calcium form A, more
preferably, the crystalline rosuvastatin calcium contains no more than about 20 % of
rosuvastatin calcium form A, as percentage area XRD. A person skilled in the art can
select a characterizing peak or a number of peaks from the known crystalline form
and quantify the polymorphic purity according to the European Pharmacopoeia
version 5.6, "Characterization of crystalline solids by XRPD", pg 4432-4437."
[00025] The invention also encompasses a process for preparing a crystalline
form of rosuvastatin calcium characterized by an X-ray powder diffraction pattern
having peaks at about 4.7, 19.4 and 22.3° 29 ± 0.2° 20 by slurrying amorphous
rosuvastatin calcium in water for more man about 10 hours.
[00026] Preferably, the water is in amount of about 2 volumes to about 50
volumes, more preferably, in an amount of about 10 volumes.
[00027] Preferably, the slurry is stirred. Preferably, the stirring is at a
temperature of about 20°C to about 40°C, more preferably, at a temperature of about
25°C. Preferably, the stirring is for about 10 to about 48 hours, more preferably, for
about 24 hours.
[00028] Preferably, the process further includes a cooling step to obtain a
precipitate. Preferably, the cooling is to a temperature of about 15°C to about 0°C,
more preferably, to a temperature of about 10°C to about 5°C.
[00029] Preferably, the precipitate upon cooling is filtered and washed to
obtain a powder. Preferably, the obtained precipitate is recovered. Preferably, the
recovery comprises isolation and washing. Preferably, the isolation is by fitration.
Preferably, the washing is with water.
[00030] The invention also encompasses a pharmaceutical composition
comprising the crystalline rosuvastatin calcium of the present invention, or a
pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable
excipient. Pharmaceutical compositions of the invention contain the crystalline
rosuvastatin calcium of the present invention, optionally in mixture with other
crystalline forms and/or other active ingredients. In certain embodiments, the
pharmaceutical compositions of the invention comprise the crystalline rosuvastatin
calcium of the present invention and less than 50%, less than 25%, less than 10%, less
than 5%, or less than 1% (by weight of all forms of rosuvastatin calcium present) of other crystalline or amorphous forms of rosuvastatin calcium. In certain embodiments, the pharmaceutical compositions of the invention comprise a therapeutically effective amount of the crystalline rosuvastatin calcium of the present invention.
[00031] The invention further encompasses a process for preparing a
pharmaceutical formulation comprising combining crystalline rosuvastatin calcium of the present invention, or a pharmaceutically acceptable salt thereof, with at least one pharmaceutically acceptable excipient.
[00032] The invention further encompasses the use of crystalline rosuvastatin
calcium of the present invention, or a pharmaceutically acceptable salt thereof, for the manufacture of a pharmaceutical composition.
[00033] Pharmaceutical compositions of the invention include excipients.
Diluents increase the bulk of a solid pharmaceutical composition, and may make a pharmaceutical dosage form containing the composition easier for the patient and care giver to handle. Diluents for solid compositions include, for example, microcrystalline cellulose (e.g. Avicel®), microfine cellulose, lactose, starch, pregelatinized starch, calcium carbonate, calcium sulfate, sugar, dextrates, dextrin, dextrose, dibasic calcium phosphate dihydrate, tribasic calcium phosphate, kaolin, magnesium carbonate, magnesium oxide, maltodextrin, mannitol, polymethacrylates (e.g. Eudragit®), potassium chloride, powdered cellulose, sodium chloride, sorbitol and talc.
[00034] Solid pharmaceutical compositions that are compacted into a dosage
form, such as a tablet, may include excipients whose functions include helping to bind the active ingredient and other excipients together after compression. Binders for solid pharmaceutical compositions include acacia, alginic acid, carbomer (e.g. carbopol), carboxymethylcellulose sodium, dextrin, ethyl cellulose, gelatin, guar gum, hydrogenated vegetable oil, hydroxyethyl cellulose, hydroxypropyl cellulose (e.g. Klucel®), hydroxypropyl methyl cellulose (e.g. Methocel®), liquid glucose, magnesium aluminum silicate, maltodextrin, methylcellulose, polymethacrylates, povidone (e.g. Kollidon®, Plasdone®), pregelatinized starch, sodium alginate and starch.
[00035] The dissolution rate of a compacted solid pharmaceutical composition
in the patient's stomach may be increased by the addition of a disintegrant to the
composition. Disintegrants include alginic acid, carboxymethylcellulose calcium, carboxymethylcellulose sodium (e.g. Ac-Di-Sol®, Primeilose®), colloidal silicon dioxide, croscarmeUose sodium, crospovidone (e.g. Kollidon®, Polyplasdone®), guar gum, magnesium aluminum silicate, methyl cellulose, microcrystalline cellulose, polacrilin potassium, powdered cellulose, pregelatinized starch, sodium'alginate, sodium starch glycolate (e.g. Explotab®) and starch.
[00036] Glidants can be added to improve the flowability of a non-compacted
solid composition and to improve the accuracy of dosing. Excipients that may function as glidants include colloidal silicon dioxide, magnesium trisilicate, powdered cellulose, starch, talc and tribasic calcium phosphate.
[00037] When a dosage form such as a tablet is made by the compaction of a
powdered composition, the composition is subjected to pressure from a punch and
dye. Some excipients and active ingredients have a tendency to adhere to the surfaces
of the punch and dye, which can cause the product to have pitting and other surface
irregularities. A lubricant can be added to the composition to reduce adhesion and
ease the release of the product from the dye. Lubricants include magnesium stearate,
calcium stearate, glyceryl monostearate, glyceryl palmitostearate, hydrogenated castor
oil, hydrogenated vegetable oil, mineral oil, polyethylene glycol, sodium benzoate,
sodium lauryl sulfate, sodium stearyl furnarate, stearic acid, talc and zinc stearate.
[00038] Flavoring agents and flavor enhancers make the dosage form more
palatable to the patient. Common flavoring agents and flavor enhancers for pharmaceutical products that may be included in the composition of the present invention include maltol, vanillin, ethyl vanillin, menthol, citric acid, furnaric acid, ethyl maltol and tartaric acid.
[00039] Solid and liquid compositions may also be dyed using any
pharmaceutically acceptable colorant to improve their appearance and/or facilitate patient identification of the product and unit dosage level.
[00040] In liquid pharmaceutical compositions of the invention, rosuvastatin
calcium and any other solid excipients are dissolved or suspended in a liquid carrier such as water, vegetable oil, alcohol, polyethylene glycol, propylene glycol or glycerin. Liquid pharmaceutical compositions may contain emulsifying agents to disperse uniformly throughout the composition an active ingredient or other excipient that is not soluble in the liquid carrier. Emulsifying agents that may be useful in liquid compositions of the present invention include, for example, gelatin, egg yolk,
casein, cholesterol, acacia, tragacanth, chondrus, pectin, methyl cellulose, carbomer, cetostearyl alcohol and cetyl alcohol.
[00041] Liquid pharmaceutical compositions may also contain a viscosity
enhancing agent to improve the mouth-feel of the product and/or coat the lining of the gastrointestinal tract. Such agents include acacia, alginic acid bentonite, carbomer, carboxymethylcellulose calcium or sodium, cetostearyl alcohol, methyl cellulose, ethylcellulose, gelatin guar gum, hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, maltodextrin, polyvinyl alcohol, povidone, propylene carbonate, propylene glycol alginate, sodium alginate, sodium starch glycolate, starch tragacanth and xanthan gum.
[00042] Sweetening agents such as sorbitol, saccharin, sodium saccharin,
sucrose, aspartame, fructose, mannitol and invert sugar may be added to improve the taste.
[00043] Preservatives and chelating agents such as alcohol, sodium benzoate,
butylated hydroxyl toluene, butylated hydroxyanisole and ethylenediamine tetraacctic
acid may be added at levels safe for ingestion to improve storage stability.
[00044] According to the invention, a liquid composition may also contain a
buffer such as guconic acid, lactic acid, citric acid or acetic acid, sodium guconate,
sodium lactate, sodium citrate or sodium acetate. Selection of excipients and the
amounts used may be readily determined by the formulation scientist based upon
experience and consideration of standard procedures and reference works in the field.
[00045] The solid compositions of the invention include powders, granulates,
aggregates and compacted compositions. The dosages include dosages suitable for oral, buccal, rectal, parenteral (including subcutaneous, intramuscular, and intravenous), inhalant and ophthalmic administration. Although the most suitable administration in any given case will depend on the nature and severity of the condition being treated, the most preferred route of the present invention is oral. The dosages maybe conveniently presented in unit dosage form and prepared by any of the methods well-known in the pharmaceutical arts.
[00046] Dosage forms include solid dosage forms like tablets, powders,
capsules, suppositories, sachets, troches and losenges, as well as liquid syrups, suspensions and elixirs. The dosage form of the invention may be a capsule containing the composition, preferably a powdered or granulated solid composition of the invention, within either a hard or soft shell. The shell may be made from gelatin
and optionally contain a plasticizer such as glycerin and sorbitol, and an opacifying
agent or colorant.
[00047] The active ingredient and excipients may be formulated into
compositions and dosage forms according to methods known in the art.
[00048] A composition for tableting or capsule filling may be prepared by wet
granulation. In wet granulation, some or all of the active ingredients and excipients in
powder form are blended and then further mixed in the presence of a liquid, typically
water, that causes the powders to clump into granules. The granulate is screened
and/or milled, dried and then screened and/or milled to the desired particle size. The
granulate may then be tableted, or other excipients may be added prior to tableting,
such as a glidant and/or a lubricant.
[00049] A tableting composition may be prepared conventionally by dry
blending. For example, the blended composition of the actives and excipients may be
compacted into a slug or a sheet and then comminuted into compacted granules. The
compacted granules may subsequently be compressed into a tablet.
[00050] As an alternative to dry granulation, a blended composition may be
compressed directly into a compacted dosage form using direct compression
techniques. Direct compression produces a more uniform tablet without granules.
Excipients that are particularly well suited for direct compression tableting include
microcrystalline cellulose, spray dried lactose, dicalcium phosphate dihydrate and
colloidal silica. The proper use of these and other excipients in direct compression
tableting is known to those in the art with experience and skill in particular
formulation challenges of direct compression tableting.
[00051 ] A capsule filling of the invention may comprise any of the
aforementioned blends and granulates that were described with reference to tableting,
however, they are not subjected to a final tableting step. The oral dosage form of the
invention is preferably in the form of an oral capsule having a dosage of about 5 mg
to about 40 mg, more preferably capsules of 5, 10, 20 and 40 mg.
[00052] The invention also encompasses a method of treatment including
administering a pharmaceutical composition containing the rosuvastatin calcium of
the present invention, or prepared from the rosuvastatin calcium of the present
invention, to a mammal in need thereof.
Solid-state Characterization
[00053] Crystalline rosuvastatin calcium of the invention was characterized by
X-Ray powder diffraction (XRD), DSC analysis and FTIR spectroscopy.
XRD
[00054] XRD Diffractograms were collected on Scintag X-Ray powder
diffractometer model X'TRA, Cu-tube, solid state detector. Scanning parameters: Range: 2-40 deg.20: continuous scan, Rate: 3.00 deg./min.
Thermal analysis
[00055] Differential Scanning Calorimetry was performed on DSC821C,
Mettler Toledo.
The crucible was crimped and punched prior to analysis. Experimental Conditions:
Sample weight: 3-5mg. Heating rate: lOdeg/min.
Examples
[00056] Example 1: Process for the preparation of crystalline form of
rosuvastatin calcium characterized by X-ray powder diffraction peaks at about 4.7. 19.4 and 22.3° 26 ±0.2° 26
A 500 ml reactor equipped with a mechanical stirrer was charged with water (150 ml) and amotphous rosuvastatin calcium (10 g). The mixture was stirred at 25 ± 5"C for 24 hours. The resulting slurry was then cooled to 5-10°C, stirred at this temperature for 1 hr, filtered and washed with water (2*10 ml) to get a powdery compound.
[00057] Having thus described the invention with reference to particular
preferred embodiments and illustrated it with Examples, those irf the art can appreciate modifications to the invention as described and illustrated that do not depart from the spirit and scope of the invention as disclosed in the specification. The Examples are set forth to aid in understanding the invention but are not intended to, and should not be construed to, limit its scope in any way. The examples do not include detailed descriptions of conventional methods. All references mentioned herein are incorporated in their entirety.

Claims:
1. A crystalline form of rosuvastatin calcium characterized by an XRD pattern with peaks at about 4.7, 19.4 and 22.3° 26 ± 0.2° 29.
2. The crystalline form of claim 1, wherein the crystalline form is further characterized with an X-ray powder diffraction with peaks at about 6.9, 11.1,15.7, 20.9 and 24.3° 26 ± 0.2° 26.
3. The crystalline form of any of the preceding claims, wherein the crystalline form is characterized by the PXRD pattern depicted in Figure 1.
4. The crystalline form of any one of claims 1 to 3, wherein the crystalline form has a DSC thermogram with an endotherm at about 132°C and another endotherm at about 220°C to about 240°C.
5. The crystalline form of any of claims 1 to 4, wherein the crystalline form is characterized by the DSC curve depicted in Figure 2.
6. The crystalline form of any of the preceding claims, wherein the crystalline form is further characterized by a TGA thermogram showing a weight loss of about of 3 to about 5 percent up to about 100°C.
7. The crystalline form of any of the preceding claims, wherein the crystalline form is characterized by the TGA curve depicted in Figure 3.
8. The crystalline form of any of the preceding claims, wherein the crystalline form contains no more than about 10 % of other polymorphic forms of rosuvastatin calcium as percentage area XRD.
9. A process for preparing a crystalline form of rosuvastatin calcium of any of the preceding claims comprising slurrying amorphous rosuvastatin calcium in water for more than about 10 hours.
10. The process of claim 9, wherein the water is in amount of about 2 volumes to about 50 volumes.
11. The process of claim 9 or 10, wherein the slurry is stirred.
12. The process of any one of claims 9 to 11, wherein the slurry is stirred for about 10 to about 48 hours.
.13. The process of any one of claims 9 to 12, herein the slurry is at a temperature of about 20°C to about 40°C.
14. The process of any one of claims 9 to 13, wherein the slurry is at a temperature of about 25°C.
15. The process of any one of claims 9 to 14, wherein the slurry is further cooled
to a temperature of about 15°C to about 0°C.
16. The process of claim 15, wherein the slurry is cooled to a temperature of
about 10°C to about 5°C.
17. The process of claim any one of claims 9-16 wherein the obtained rosuvastatin calcium is recovered.
18. The process of claim 17, wherein the recovery comprises isolation and washing..
19. The process of claim 18, wherein the isolation is by filtration.
20. The process of claim 18 or 19, wherein the washing is with water.
21. A pharmaceutical composition comprising the crystalline rosuvastatin calcium of any of claims 1-8 and at least one pharmaceutically acceptable excipient.
22. The use of crystalline rosuvastatin calcium of any of claims 1 -8 in the manufacture of a medicament for lowering cholesterol in a mammal.