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Dendritic Cells Targeting Vaccine

Abstract: ABSTRACT DENDRITIC CELLS-TARGETING VACCINE The present disclosure relates to recombinant single chain fragment variable (ScFv) binding to DEC-205 of dendritic cells having amino acid sequence selected from a group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, and SEQ ID NO: 9. Also disclosed are ScFv-antigen complex, a method for inducing immune response in a subject using the ScFv-antigen complex and a vaccine composition comprising the ScFv-antigen complex. The ScFv-antigen complex as disclosed herein can be used as immuno-contraceptives for mammals.

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Notices, Deadlines & Correspondence

Patent Information

Application #
Filing Date
27 October 2017
Publication Number
18/2019
Publication Type
INA
Invention Field
BIOTECHNOLOGY
Status
Email
iprdel@lakshmisri.com
Parent Application

Applicants

INDIAN INSTITUTE OF SCIENCE
Office of Intellectual Property and Technology Licensing, Indian Institute of Science, Bangalore, Karnataka-560012, India.

Inventors

1. DIGHE, Rajan Ramakant
Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India.
2. SINHA, Shakun
Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India.

Specification

I/We Claim:
1. A recombinant single chain fragment variable (ScFv) binding to DEC-205 of
dendritic cells, said ScFv comprising:
a) a heavy chain variable region comprising CDRH1, CDRH2 and CDRH3, wherein the CDRH1 is selected from a group consisting of SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, and SEQ ID NO: 14, CDRH2 is selected from a group consisting of SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, and SEQ ID NO: 21, and CDRH3 is selected from a group consisting of SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, and SEQ ID NO: 26; and
b) a light chain variable region comprising CDRL1, CDRL2 and CDRL3, wherein the CDRL1 is selected from a group consisting of SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, and SEQ ID NO: 31, CDRL2 is selected from a group consisting of SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, and SEQ ID NO: 37, and CDRL3 is selected from a group consisting of SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, and SEQ ID NO: 45,
wherein the heavy chain variable region and the light chain variable region is linked with a linker molecule having amino acid sequence as represented by SEQ ID NO: 10.
2. The recombinant ScFv as claimed in claim 1, wherein the ScFv has amino acid sequence selected from a group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, and SEQ ID NO: 9.
3. The recombinant ScFv as claimed in claim 1, wherein the ScFv comprises:

a) a heavy chain variable region comprising CDRH1 having amino acid sequence as depicted in SEQ ID NO: 11, CDRH2 having amino acid sequence as depicted in SEQ ID NO: 15, and CDRH3 having amino acid sequence as depicted in SEQ ID NO: 22; and
b) a light chain variable region comprising CDRL1 having amino acid sequence as depicted in SEQ ID NO: 27, CDRL2 having amino acid sequence as depicted in SEQ ID NO: 32, and CDRL3 having amino acid sequence as depicted in SEQ ID NO: 38,
wherein the heavy chain variable region and the light chain variable region is linked with a linker molecule having amino acid sequence as represented by SEQ ID NO: 10, and the ScFv has an amino acid sequence as depicted in SEQ ID NO: 1.
4. The recombinant ScFv as claimed in claim 1, wherein the ScFv comprises:
a) a heavy chain variable region comprising CDRH1 having amino acid sequence as depicted in SEQ ID NO: 11, CDRH2 having amino acid sequence as depicted in SEQ ID NO: 16, and CDRH3 having amino acid sequence as depicted in SEQ ID NO: 22; and
b) a light chain variable region comprising CDRL1 having amino acid sequence as depicted in SEQ ID NO: 28, CDRL2 having amino acid sequence as depicted in SEQ ID NO: 32, and CDRL3 having amino acid sequence as depicted in SEQ ID NO: 39,
wherein the heavy chain variable region and the light chain variable region is linked with a linker molecule having amino acid sequence as represented by SEQ ID NO: 10, and the ScFv has an amino acid sequence as depicted in SEQ ID NO: 2.
5. The recombinant ScFv as claimed in claim 1, wherein the ScFv comprises:

a) a heavy chain variable region comprising CDRH1 having sequence as depicted in SEQ ID NO: 12, CDRH2 having amino acid s depicted in SEQ ID NO: 17, and CDRH3 having amino acid sequence as SEQ ID NO: 22; and
b) a light chain variable region comprising CDRL1 having amino ac as depicted in SEQ ID NO: 27, CDRL2 having amino acid sequence as SEQ ID NO: 33 and CDRL3 having amino acid sequence as depicted in S 40,
wherein the heavy chain variable region and the light chain variable regi with a linker molecule having amino acid sequence as represented by S 10, and the ScFv has an amino acid sequence as depicted in SEQ ID NO: 3
6. The recombinant ScFv as claimed in claim 1, wherein the ScFv co
a) a heavy chain variable region comprising CDRH1 having sequence as depicted in SEQ ID NO: 13, CDRH2 having amino acid s depicted in SEQ ID NO: 18, and CDRH3 having amino acid sequence as SEQ ID NO: 23; and
b) a light chain variable region comprising CDRL1 having amino ac as depicted in SEQ ID NO: 29, CDRL2 having amino acid sequence as SEQ ID NO: 34, and CDRL3 having amino acid sequence as depicted NO: 41,
wherein the heavy chain variable region and the light chain variable regi with a linker molecule having amino acid sequence as represented by S 10, and the ScFv has an amino acid sequence as depicted in SEQ ID NO: 4
7. The recombinant ScFv as claimed in claim 1, wherein the ScFv co

a) a heavy chain variable region comprising CDRH1 having amino acid sequence as depicted in SEQ ID NO: 14, CDRH2 having amino acid sequence as depicted in SEQ ID NO: 19, and CDRH3 having amino acid sequence as depicted in SEQ ID NO: 24; and
b) a light chain variable region comprising CDRL1 having amino acid sequence as depicted in SEQ ID NO: 30, CDRL2 having amino acid sequence as depicted in SEQ ID NO: 35, and CDRL3 having amino acid sequence as depicted in SEQ ID NO: 42,
wherein the heavy chain variable region and the light chain variable region is linked with a linker molecule having amino acid sequence as represented by SEQ ID NO: 10, and the ScFv has an amino acid sequence as depicted in SEQ ID NO: 5.
8. The recombinant ScFv as claimed in claim 1, wherein the ScFv comprises:
a) a heavy chain variable region comprising CDRH1 having amino acid sequence as depicted in SEQ ID NO: 13, CDRH2 having amino acid sequence as depicted in SEQ ID NO: 20, and CDRH3 having amino acid sequence as depicted in SEQ ID NO: 25; and
b) a light chain variable region comprising CDRL1 having amino acid sequence as depicted in SEQ ID NO: 31, CDRL2 having amino acid sequence as depicted in SEQ ID NO: 36, and CDRL3 having amino acid sequence as depicted in SEQ ID NO: 43,
wherein the heavy chain variable region and the light chain variable region is linked with a linker molecule having amino acid sequence as represented by SEQ ID NO: 10, and the ScFv has an amino acid sequence as depicted in SEQ ID NO: 6.
9. The recombinant ScFv as claimed in claim 1, wherein the ScFv comprises:

a) a heavy chain variable region comprising CDRH1 having amino acid sequence as depicted in SEQ ID NO: 13, CDRH2 having amino acid sequence as depicted in SEQ ID NO: 18, and CDRH3 having amino acid sequence as depicted in SEQ ID NO: 23; and
b) a light chain variable region comprising CDRL1 having amino acid sequence as depicted in SEQ ID NO: 29, CDRL2 having amino acid sequence as depicted in SEQ ID NO: 34, and CDRL3 having amino acid sequence as depicted in SEQ ID NO: 41,
wherein the heavy chain variable region and the light chain variable region is linked with a linker molecule having amino acid sequence as represented by SEQ ID NO: 10, and the ScFv has an amino acid sequence as depicted in SEQ ID NO: 7.
10. The recombinant ScFv as claimed in claim 1, wherein the ScFv comprises:
a) a heavy chain variable region comprising CDRH1 having amino acid sequence as depicted in SEQ ID NO: 13, CDRH2 having amino acid sequence as depicted in SEQ ID NO: 20, and CDRH3 having amino acid sequence as depicted in SEQ ID NO: 25; and
b) a light chain variable region comprising CDRL1 having amino acid sequence as depicted in SEQ ID NO: 31, CDRL2 having amino acid sequence as depicted in SEQ ID NO: 36, and CDRL3 having amino acid sequence as depicted in SEQ ID NO: 44,
wherein the heavy chain variable region and the light chain variable region is linked with a linker molecule having amino acid sequence as represented by SEQ ID NO:
10, and the ScFv has an amino acid sequence as depicted in SEQ ID NO:8.
11. An ScFv-antigen complex, comprising the ScFv as claimed in any one of the
claims 1-10, wherein the ScFv is linked to an antigen.

12. The ScFv-antigen complex as claimed in claim 11, wherein the ScFv is linked to the antigen through method selected from a group consisting of non-covalent biological interaction, chemical cross linking, and synthetic biological techniques.
13. The ScFv-antigen complex as claimed in claim 12, wherein the ScFv is linked to the antigen through non-covalent biological interaction.
14. The ScFv-antigen complex as claimed in claim 13, wherein the ScFv is linked to the antigen through streptavidin-biotin interaction.
15. The ScFv-antigen complex as claimed any one of the claims 11-14, wherein the antigen is selected from a group consisting of gonadotropins, cancer antigens, viral antigens and the antigens from the pathogenic organisms.
16. The ScFv-antigen complex as claimed in claim 15, wherein the antigen is gonadotropin selected from a group consisting of human FSH, human LH, human chorionic gonadotropin, human FSHβ subunit, human CGβ subunit, human LHβ subunit, bovine FSH, bovine LH, β subunits of bovine FSH and LH, bovine FSH, bovine LH, β subunits of bovine FSH and LH, GnRH and its analogs.
17. The ScFv-antigen complex as claimed in claim 16, wherein the complex is used as a contraceptive vaccine for mammals.
18. The ScFv-antigen complex as claimed in any one of the claims 11-17 is used for targeted delivery of the antigen to dendritic cells.
19. A method for inducing immune response in a subject, comprising:

a) obtaining the ScFv-antigen complex as claimed in any one of the claims 11 -18; and
b) administering to the subject an immunogenic effective amount of the ScFv-antigen complex,
wherein the ScFv-antigen complex induces immune response in the subject.

20. A vaccine composition comprising the ScFv-antigen complex as claimed in any one of the claims 11-18.
21. The vaccine composition as claimed in claim 20, wherein the antigen is gonadotropin, and the vaccine is used as contraceptive for mammals.

Documents

Application Documents

# Name Date
1 201741038286-STATEMENT OF UNDERTAKING (FORM 3) [27-10-2017(online)].pdf 2017-10-27
2 201741038286-PROVISIONAL SPECIFICATION [27-10-2017(online)].pdf 2017-10-27
3 201741038286-FORM 1 [27-10-2017(online)].pdf 2017-10-27
4 201741038286-DRAWINGS [27-10-2017(online)].pdf 2017-10-27
5 201741038286-FORM-26 [20-11-2017(online)].pdf 2017-11-20
6 Correspondence by Agent_Power of Attorney_21-11-2017.pdf 2017-11-21
7 201741038286-Proof of Right (MANDATORY) [26-04-2018(online)].pdf 2018-04-26
8 Correspondence by Agent_Form 1_01-05-2018.pdf 2018-05-01
9 201741038286-SEQUENCE LISTING (.txt) [26-10-2018(online)].txt 2018-10-26
10 201741038286-DRAWING [26-10-2018(online)].pdf 2018-10-26
11 201741038286-CORRESPONDENCE-OTHERS [26-10-2018(online)].pdf 2018-10-26
12 201741038286-COMPLETE SPECIFICATION [26-10-2018(online)].pdf 2018-10-26
13 201741038286-Request Letter-Correspondence [31-10-2018(online)].pdf 2018-10-31
14 201741038286-Form 1 (Submitted on date of filing) [31-10-2018(online)].pdf 2018-10-31
15 201741038286-CERTIFIED COPIES TRANSMISSION TO IB [31-10-2018(online)].pdf 2018-10-31
16 Correspondence by Agent_Sequence Listing in CD_02-11-2018.pdf 2018-11-02
17 201741038286-FORM 18A [26-07-2019(online)].pdf 2019-07-26
18 201741038286-FER.pdf 2019-08-26
19 201741038286-PETITION UNDER RULE 137 [25-02-2020(online)].pdf 2020-02-25
20 201741038286-FORM 3 [25-02-2020(online)].pdf 2020-02-25
21 201741038286-FER_SER_REPLY [25-02-2020(online)].pdf 2020-02-25
22 201741038286-US(14)-HearingNotice-(HearingDate-27-07-2020).pdf 2020-06-25
23 201741038286-Correspondence to notify the Controller [24-07-2020(online)].pdf 2020-07-24
24 201741038286-Written submissions and relevant documents [10-08-2020(online)].pdf 2020-08-10
25 201741038286-FORM 3 [11-09-2020(online)].pdf 2020-09-11

Search Strategy

1 ISAIN2018001407_26-08-2019.pdf
2 ISAIN2018001407sequencesearch_26-08-2019.pdf