Abstract: A device for extraction of kojic acid from kojic acid producing fungi, comprising a housing 101 with motorized tracked wheel arrangement 102 positioned on an agricultural field, a user-interface to input command for extracting kojic acid, a first imaging unit 103 deteacts presence of fungal growth, a four-bar linkage arrangement 104 with a bucket 105 transfer soil to a chamber 120, a gripper 106 places an agar plate 107 in a receptacle 109, a first robotic arm 110 transfer a small amount of soil on agar plate 107 via a spatula 111, a motorized lid 112 allows growth of fungi on plate 107, a timer monitors time duration, a first flask 115 with an oxygen sensor confirms presence of kojic acid, a platform 116 with a flask-shaped body 117 allow extraction of kojic acid, a second imaging unit 118 detects growth of fungi, and a evaporator unit 119 evaporates solvent.
Description:FIELD OF THE INVENTION
[0001] The present invention relates to a device for extraction of kojic acid from kojic acid producing fungi, designed to assist users in extracting kojic acid from kojic acid-extracting fungi by automating the collection of soil from agricultural fields with mangrove soil, facilitating the cultivation of fungi under controlled conditions, and enabling the efficient extraction of kojic acid.
BACKGROUND OF THE INVENTION
[0002] The extraction of kojic acid from kojic acid-extracting fungi holds significant importance in various industries, particularly in cosmetics, pharmaceuticals, and food preservation. Kojic acid, a naturally derived compound, is highly valued for its ability to inhibit melanin extraction, making it a popular ingredient in skin-lightening products. Additionally, it possesses antioxidant, anti-inflammatory, and antimicrobial properties, which make it beneficial in treating hyperpigmentation, acne, and other skin conditions. Beyond cosmetics, kojic acid is also used in the food industry as a preservative, owing to its ability to prevent the growth of harmful microorganisms. The extraction process, typically carried out through the fermentation of specific fungi, requires efficient methods for cultivating the fungi and extracting the acid. By developing automated devices to streamline the process, the extraction of kojic acid can be more sustainable, cost-effective, and scalable, meeting the growing demand for this versatile compound while ensuring consistent quality and purity.
[0003] Traditional methods of extracting kojic acid from kojic acid-extracting fungi typically involve the fermentation of specific fungi, such as Aspergillus oryzae or Penicillium spp., under controlled conditions in large fermenters. The process requires maintaining optimal environmental factors like temperature, pH, and nutrient supply to encourage fungal growth and kojic acid extraction. After fermentation, the culture broth is harvested, and the kojic acid is extracted through solvent extraction or precipitation methods. While effective, these traditional methods have several drawbacks. They often require significant labor and time for monitoring and controlling environmental conditions, leading to inefficiencies. Moreover, the scale of extraction is limited by the manual processes involved, making it challenging to meet the growing demand for kojic acid. Additionally, the extraction methods can be wasteful, extracting by-products that may require further processing. These challenges result in higher extraction costs, inconsistent yields, and limitations in scalability for industrial applications.
[0004] CN110218750A provides aspergillus versicolor bacterial strains to apply in fermenting and producing kojic acid, specifically includes and aspergillus versicolor is inoculated in fermentation medium, and 25-32 DEG C is protected from light culture 30-50 days;Fermentation material is extracted using reagent cold soaking is extracted further, fermentation is obtained and extracts medicinal extract;Medicinal extract first is extracted into fermentation and carries out normal phase column chromatography eluant, obtains crude kojic acid using macroreticular resin chromatography afterwards;It is filtered after crude kojic acid is dissolved by heating, last cooling crystallization obtains kojic acid sterling.
[0005] CN110407786A discloses a kind of extracting method of the kojic acid of environmental protection and energy saving, extracting method includes step flocculation filtration, ion exchange, crystallization and dry finished product kojic acid;The flocculation filtration step includes: plate compression: obtaining filters pressing clear liquid and filter cake after nontoxic flocculant flocculation, plate compression are added into kojic acid fermentation liquid;Protein feed is obtained after cake dewatering is dry;Ultrafiltration: after filters pressing clear liquid ultrafiltration, ultrafiltration clear liquid and ultrafiltration turbid are obtained;Nanofiltration: after ultrafiltration clear liquid nanofiltration, nanofiltration turbid and the nanofiltration clear liquid to ion-exchange step processing are obtained;It further include RO film concentration step between ion exchange and crystallization decoloration;The RO film concentration includes: the concentration of low temperature RO film and the concentration of medium temperature RO film.The circulation that the extracting method of kojic acid of the invention realizes kojic acid fermentation liquid is extracted, and extraction recovery rate is higher, and no production discharge of wastewater is more environmentally friendly;Use reverse osmosis concentration, more energy efficient;It is decolourized using nanofiltration membrane, the kojic acid light transmittance extracted is higher.
[0006] Conventionally, many devices have disclosed methods for extracting kojic acid, primarily from fungi, but these devices do not assist users in automating the collection of soil, particularly from agricultural fields enriched with mangrove soil, which is crucial for efficient kojic acid extraction. By automating the process of collecting soil from these specialized areas, such devices could optimize the extraction process, ensuring better yields and consistency in the kojic acid derived from the fungi. The absence of such automation limits the scalability and efficiency of current kojic acid extraction methods, leaving room for improvements in the process.
[0007] To overcome the aforementioned drawbacks, there is a need in the art to develop a device specifically designed for the extraction of kojic acid from kojic acid-extracting fungi, which assists users by automating the collection of soil from agricultural fields containing mangrove soil, known to enhance the growth of the fungi. This device would facilitate the cultivation of the fungi under controlled conditions, optimizing the extraction process, increasing efficiency, and improving the consistency of kojic acid yields. By automating soil collection and cultivation, the device would significantly streamline the production process, enhancing both scalability and reliability of kojic acid extraction.
OBJECTS OF THE INVENTION
[0008] The principal object of the present invention is to overcome the disadvantages of the prior art.
[0009] An object of the present invention is to develop a device that helps users extract kojic acid from kojic acid-extracting fungi by collecting soil from an agricultural field with mangrove soil, facilitating the cultivation and processing of fungi under controlled conditions for efficient kojic acid extraction.
[0010] Another object of the present invention is to develop a device that extracts kojic acid from kojic acid-extracting fungi, including soil collection, fungal cultivation, and processing, ensuring efficient and precise extraction in a controlled environment.
[0011] Yet another object of the present invention is to develop a portable and reliable device that is capable of automatically maneuvering in the field for collection of soil having kojic acid-extracting fungi.
[0012] The foregoing and other objects, features, and advantages of the present invention will become readily apparent upon further review of the following detailed description of the preferred embodiment as illustrated in the accompanying drawings.
SUMMARY OF THE INVENTION
[0013] The present invention relates to a device for extraction of kojic acid from kojic acid producing fungi that assists users in extracting kojic acid from kojic acid-extracting fungi by automating the collection of soil from agricultural fields with mangrove soil, enabling efficient fungal cultivation and kojic acid extraction under controlled conditions.
[0014] According to an embodiment of the present invention, a device for extraction of kojic acid from kojic acid producing fungi, comprising a housing with motorized tracked wheel arrangement is positioned on an agricultural field having mangrove soil, a user-interface inbuilt in a computing unit wirelessly associated with the device enables the user to give input command for extracting kojic acid from fungus grown on the soil, a first artificial intelligence-based imaging unit mounted on the housing for detecting presence of fungal growth on the soil, a four-bar linkage arrangement configured on the housing and equipped with a bucket transfer soil to a chamber installed at ceiling portion of the housing, a Peltier unit configured with the chamber maintains optimum temperature of the chamber to allow growth of the fungi, a telescopically operated gripper installed within the housing places an agar plate from a container mounted within the housing in a receptacle arranged within the housing, a first robotic arm assembled within the housing transfer a small amount of the soil on the agar plate via a equipped spatula, a motorized lid configured with the receptacle allows growth of the fungi on the agar plate for a pre-set time duration, a second robotic arm arranged within the housing and equipped with an inoculating loop, a timer integrated within the microcontroller monitors time duration, a first flask positioned on base of the housing with an oxygen sensor detects release of oxygen confirming presence of kojic acid extracting fungi, a platform positioned within the housing with a flask-shaped body allow growth of the fungi and extraction of kojic acid, a second artificial intelligence-based imaging unit mounted within the housing for detecting growth of fungi in the body, and a rotary evaporator unit embedded within the housing evaporate solvent (ethyl acetate) from the body and obtaining kojic acid in powdered form.
[0015] While the invention has been described and shown with particular reference to the preferred embodiment, it will be apparent that variations might be possible that would fall within the scope of the present invention.
BRIEF DESCRIPTION OF THE DRAWINGS
[0016] These and other features, aspects, and advantages of the present invention will become better understood with regard to the following description, appended claims, and accompanying drawings where:
Figure 1 illustrates an inner view of a device for extraction of kojic acid from kojic acid producing fungi.
DETAILED DESCRIPTION OF THE INVENTION
[0017] The following description includes the preferred best mode of one embodiment of the present invention. It will be clear from this description of the invention that the invention is not limited to these illustrated embodiments but that the invention also includes a variety of modifications and embodiments thereto. Therefore, the present description should be seen as illustrative and not limiting. While the invention is susceptible to various modifications and alternative constructions, it should be understood, that there is no intention to limit the invention to the specific form disclosed, but, on the contrary, the invention is to cover all modifications, alternative constructions, and equivalents falling within the spirit and scope of the invention as defined in the claims.
[0018] In any embodiment described herein, the open-ended terms "comprising," "comprises,” and the like (which are synonymous with "including," "having” and "characterized by") may be replaced by the respective partially closed phrases "consisting essentially of," consists essentially of," and the like or the respective closed phrases "consisting of," "consists of, the like.
[0019] As used herein, the singular forms “a,” “an,” and “the” designate both the singular and the plural, unless expressly stated to designate the singular only.
[0020] The present invention relates to a device for extraction of kojic acid from kojic acid producing fungi that aids users in extracting kojic acid from kojic acid- producing fungi by automating the collection of soil from agricultural fields with mangrove soil, facilitating the cultivation of fungi under controlled conditions, and enabling the efficient extraction of kojic acid for various industrial and cosmetic applications.
[0021] Referring to Figure 1, an inner view of a device for extraction of kojic acid from kojic acid producing fungi is illustrated, comprising a housing 101 installed with motorized tracked wheel arrangement 102, a first artificial intelligence-based imaging unit 103 mounted on said housing 101, a four-bar linkage arrangement 104 configured on said housing 101 and equipped with a bucket 105, a chamber 120 installed at ceiling portion of said housing 101, a telescopically operated gripper 106 installed within said housing 101, an agar plate 107 in a container 108 mounted within said housing 101, a receptacle 109 arranged within said housing 101, a first robotic arm 110 assembled within said housing 101 with a spatula 111, a motorized lid 112 configured with said receptacle 109, a second robotic arm 113 arranged within said housing 101 and equipped with an inoculating loop 114, a first flask 115 positioned on base of said housing 101, a platform 116 positioned within said housing 101 and placed with a flask-shaped body 117, a second artificial intelligence-based imaging unit 118 mounted within said housing 101 and a rotary evaporator unit 119 embedded within said housing 101.
[0022] The device proposed herein includes a housing 101 positioned on an agricultural field having mangrove soil for extraction of kojic acid from kojic acid extracting fungi. The housing 101 as mentioned herein is a cuboidal enclosure encasing various components associated with the device, wherein the housing 101 is made up of material that includes but not limited to stainless steel, which in turn ensures that the device is of generous size and is light in weight.
[0023] The housing 101 is equipped with a motorized tracked wheel arrangement 102 in association with a microcontroller, wherein the wheels are installed with support of multiple rod like structure to maneuver the housing 101 throughout the field. The supporting rods helps to maintain an optimum distance between the base of the housing 101 and the field to enable the device to supervise the condition of the field.
[0024] In order to activate functioning of the device, a user is required to manually switch on the device by pressing a button positioned on the housing 101, wherein the button used herein is a push button. Upon pressing of the button, the circuits get closed allowing conduction of electricity that leads to activation of the device and vice versa.
[0025] Upon activation of the device by the user, a user-interface installed within a computing unit is accessed by the user to input commands regarding a requirement of extracting kojic acid from fungus grown on the soil. The user-interface provides a series of questions regarding extracting kojic acid from fungus grown on the soil. The user either selects from a list of options provided on the display or manually enters the details, wherein the user is required to enter details such as a requirement of extracting kojic acid from fungus grown on the soil.
[0026] The computing unit is wirelessly linked with the microcontroller via a communication module. The communication module mentioned herein includes, but not limited to Wi-Fi (Wireless Fidelity) module, Bluetooth module, GSM (Global System for Mobile Communication) module for enabling the user to input commands regarding a requirement of extracting kojic acid from fungus grown on the soil.
[0027] In response to input commands of the user, the microcontroller actuates the wheel arrangement 102 for maneuvering the housing 101 on the field. The motorized tracked wheel operates by using a motor to drive a sprocket engaged with the wheel. The sprocket engages with a continuous track, consisting of interlocking links or treads, providing traction and stability. Upon actuation of the wheel by the microcontroller, the motor rotates the sprocket that in turn moves the wheels, thereby propelling the housing 101 over the field. The microcontroller regulates the speed and direction of the motor to control the translation to the housing 101 over the field.
[0028] The microcontroller synchronously activates a first artificial intelligence-based imaging unit 103 mounted on the housing 101 for detecting presence of fungal growth on the soil. The imaging unit 103 comprises of an image capturing arrangement 102 including a set of lenses that captures multiple images in surrounding of the housing 101, and the captured images are stored within memory of the imaging unit 103 in form of an optical data. The imaging unit 103 also comprises of a processor that is integrated with artificial intelligence protocols, such that the processor processes the optical data and extracts the required data from the captured images. The extracted data is further converted into digital pulses and bits and are further transmitted to the microcontroller. The microcontroller processes the received data and determines presence of fungal growth on the soil.
[0029] In response to the determined presence of fungal growth on the soil, the microcontroller actuates a four-bar linkage arrangement 104 configured on the housing 101 for collecting the soil from the ground surface via a bucket 105 equipped with the arrangement 104. The four-bar linkage arrangement 104 involves a set-up of four rigid bars that are connected in a loop 114, allowing controlled movement of a bucket 105. The motor drives one of the bars, creating motion that is transferred through the linkage to manipulate the bucket’s 105 position. As the arrangement 104 operates, the bucket 105 is lowered to the ground, scooping up soil from the surface, and the microcontroller then directs the arrangement 104 to transfer the soil to a chamber 120 installed at ceiling portion of the housing 101.
[0030] Upon accommodation of soil into the chamber 120, a Peltier unit configured with the chamber 120 is activated by the microcontroller for maintaining an optimum temperature of the chamber 120 to allow optimum growth of the fungi. The Peltier unit consists of two semiconductor plate 107s, known as Peltier plate 107s, connected in series and sandwiched between two ceramic plate 107. When an electric current is applied to the Peltier unit, one side of the unit absorbs heat from its surroundings, while the other side releases heat, thereby maintaining an optimum temperature of the chamber 120 to allow growth of the fungi.
[0031] A telescopically operated gripper 106 installed within the housing 101 is then actuated by the microcontroller for gripping an agar plate 107 from a container 108 mounted within the housing 101. The telescopically operated gripper 106 includes a gripper 106 linked to a pneumatic unit, including an air compressor, air cylinders, air valves and piston which works in collaboration to aid in extension and retraction of the gripper 106. The pneumatic unit is operated by the microcontroller, such that the microcontroller actuates valve to allow passage of compressed air from the compressor within the cylinder, the compressed air further develops pressure against the piston and results in pushing and extending the piston. The piston is connected with the gripper 106 and due to applied pressure the gripper 106 extends and similarly, the microcontroller retracts the telescopically operated gripper 106 by closing the valve resulting in retraction of the piston. Thus, the microcontroller regulates the extension/retraction of the gripper 106 in order to grip agar plate 107 from the container 108 and the microcontroller subsequently directs the arm 110 for placing the agar plate 107 in a receptacle 109 arranged within the housing 101.
[0032] A first robotic arm 110 assembled within the housing 101 is actuated by the microcontroller for transferring a small amount of the soil on the agar plate 107 via a spatula 111 equipped with the first robotic arm 110. The first robotic arm 110 comprises of a robotic link and a clamp attached to the link. The robotic link is made of several segments that are attached together by joints also referred to as axes. Each joint of the segments contains a step motor that rotates and allows the robotic link to complete a specific motion of the arm 110. Upon actuation of the robotic arm 110 by the microcontroller, the motor drives the movement of the clamp to transfer a small amount of the soil on the agar plate 107 via the spatula 111.
[0033] Upon collection of soil on the agar plate 107, the microcontroller then actuates a motorized lid 112 configured with the receptacle 109 to close the opening, for allowing growth of the fungi on the agar plate 107 for a pre-set time duration. The iris lid 112 typically refers to the iris or aperture mechanism in the camera or optical instruments as it works in a similar manner to that of a human eye. The iris consists several thin and overlapping blades that forms an adjustable opening of the lid 112. Upon actuation of the iris lid 112 by the microcontroller the blades moves together resulting in the closing of mouth portion of the lid 112 for allowing growth of the fungi on the agar plate 107 for a pre-set time duration.
[0034] The microcontroller monitors completion of the pre-set time duration via a timer integrated within the microcontroller. The timer includes a RTC (real time clock) comprises of a controller, oscillator and an embedded quartz crystal resonator. The function of RTC (real time clock) is to keep accurate track of time even when a power supply is turned off or the device is placed in low power mode.
[0035] Upon completion of the pre-set time duration, the microcontroller actuates the lid 112 to open and a second robotic arm 113 arranged within the housing 101 and equipped with an inoculating loop 114 for collecting small amount of fungi from the agar plate 107 to a first flask 115 positioned on base of the housing 101. The movement of the second robotic arm 113 is regulated by the microcontroller in the same manner as the first robotic arm 110 for collecting small amount of fungi from the agar plate 107 and transferring it to the first flask 115.
[0036] The first flask 115 is stored with ferric chloride solution for catalyst test of the fungi, such that an oxygen sensor arranged on mouth potion of the first flask 115 detects release of oxygen confirming presence of kojic acid extracting fungi. The oxygen sensor detects release of oxygen in a flask 115 containing ferric chloride solution works by monitoring changes in oxygen levels as a result of microbial activity. When the flask 115 is stored with a ferric chloride solution and exposed to kojic acid-extracting fungi, the fungi metabolize the ferric chloride and extract kojic acid, which catalyzes the reduction of ferric ions (Fe^3+) to ferrous ions (Fe^2+). This process releases oxygen as a byproduct. The oxygen sensor detects this increase in oxygen concentration, enabling the microcontroller to confirm the presence of kojic acid-extracting fungi.
[0037] Upon confirming the presence of kojic acid extracting fungi, the microcontroller re-actuates the second robotic arm 113 for collecting fungi from the plate 107 and transferring the fungi to a flask-shaped body 117 stored with ethyl acetate and assembled over the body 117 a platform 116 positioned within the housing 101 for allowing growth of the fungi and extraction of kojic acid.
[0038] A second artificial intelligence-based imaging unit 118 mounted within the housing 101 is then activated by the microcontroller for detecting growth of fungi in the body 117. The operation of the second artificial intelligence-based imaging unit 118 is performed in the same manner as the first artificial intelligence-based imaging unit 103 for detecting growth of fungi in the body 117.
[0039] Upon detecting growth of the fungi, upto a threshold level, the microcontroller actuates the gripper 106 for placing the body 117 in a rotary evaporator unit 119 embedded within the housing 101 and subsequently activates the rotary evaporator unit 119 for evaporating solvent (ethyl acetate) from the body 117 and obtaining kojic acid in powdered form. The rotary evaporator unit 119 operates by first activating the unit to initiate solvent evaporation. The body 117 containing the solution (ethyl acetate and kojic acid) is placed in the rotary flask, which is then rotated while being gently heated. Reduced pressure inside the housing 101 lowers the boiling point of ethyl acetate, allowing it to evaporate. The vapor is condensed and collected separately, leaving behind the kojic acid. After the solvent is fully evaporated, the remaining kojic acid is obtained as a concentrated powder.
[0040] Upon activation of the rotary evaporator unit 119 for a threshold time limit, the microcontroller terminates working of the rotary evaporator unit 119, followed by sending an alert on the computing unit for notifying the user to collect the kojic acid from the body 117.
[0041] Lastly, a battery is installed within the device which is connected to the microcontroller that supplies current to all the electrically powered components that needs an amount of electric power to perform their functions and operation in an efficient manner. The battery utilized here, is preferably a dry battery which is made up of Lithium-ion material that gives the device a long-lasting as well as an efficient DC (Direct Current) current which helps every component to function properly in an efficient manner. As the device is battery operated and do not need any electrical voltage for functioning. Hence the presence of battery leads to the portability of the device i.e., user is able to place as well as moves the device from one place to another as per the requirements.
[0042] The present invention works best in the following manner, where the housing 101 positioned on the agricultural field having mangrove soil for extraction of kojic acid from kojic acid extracting fungi. Upon activation of the device by the user, the user-interface is accessed by the user to input commands regarding the requirement of extracting kojic acid from fungus grown on the soil. In response to input commands of the user, the microcontroller actuates the wheel arrangement 102 for maneuvering the housing 101 on the field. The microcontroller synchronously activates the first artificial intelligence-based imaging unit 103 for detecting presence of fungal growth on the soil. In response to the determined presence of fungal growth on the soil, the microcontroller actuates the four-bar linkage arrangement 104 for collecting the soil from the ground surface into the chamber 120 via the bucket 105. Upon accommodation of soil into the chamber 120, the Peltier unit is activated by the microcontroller for maintaining the optimum temperature of the chamber 120 to allow growth of the fungi. the telescopically operated gripper 106 is then actuated by the microcontroller for gripping the agar plate 107 for placing the agar plate 107 in the receptacle 109 arranged within the housing 101.
[0043] In continuation, the first robotic arm 110 is actuated by the microcontroller for transferring the small amount of the soil on the agar plate 107 via the spatula 111. Upon collection of soil on the agar plate 107, the microcontroller then actuates the motorized lid 112 to close for allowing growth of the fungi on the agar plate 107 for the pre-set time duration. The microcontroller monitors completion of the pre-set time duration via the timer. Upon completion of the pre-set time duration, the microcontroller actuates the lid 112 to open and the second robotic arm 113 with the inoculating loop 114 for collecting small amount of fungi from the agar plate 107 to the first flask 115 p. The first flask 115 is stored with ferric chloride solution for catalyst test of the fungi, such that the oxygen sensor detects release of oxygen confirming presence of kojic acid extracting fungi. Upon confirming the presence of kojic acid extracting fungi, the microcontroller re-actuates the second robotic arm 113 for collecting fungi from the plate 107 and transferring the fungi to the flask-shaped body 117 stored with ethyl acetate and assembled over the body 117 the platform 116 positioned within the housing 101 for allowing growth of the fungi and extraction of kojic acid. A second artificial intelligence-based imaging unit 118 is then activated by the microcontroller for detecting growth of fungi in the body 117. Upon activation of the rotary evaporator unit 119 for the threshold time limit, the microcontroller terminates working of the rotary evaporator unit 119, followed by sending the alert on the computing unit for notifying the user to collect the kojic acid from the body 117.
[0044] Although the field of the invention has been described herein with limited reference to specific embodiments, this description is not meant to be construed in a limiting sense. Various modifications of the disclosed embodiments, as well as alternate embodiments of the invention, will become apparent to persons skilled in the art upon reference to the description of the invention. , C , Claims:1) A device for extraction of kojic acid from kojic acid producing fungi, comprising:
i) a housing 101 positioned on an agricultural field having mangrove soil, installed with motorized tracked wheel arrangement 102 for maneuvering said housing 101 on said field, wherein a user-interface inbuilt in a computing unit is wirelessly associated with said device for enabling a user to give input command for extracting kojic acid from fungus grown on said soil;
ii) a microcontroller wirelessly linked with said computing unit that processes said input commands and actuates said wheel arrangement 102 for maneuvering said housing 101 on said field, wherein a first artificial intelligence-based imaging unit 103 paired with a processor is mounted on said housing 101 for capturing and processing multiple images of said soil, respectively, for detecting presence of fungal growth on said soil;
iii) a four-bar linkage arrangement 104 configured on said housing 101 and equipped with a bucket 105 that is actuated by said microcontroller for collecting a portion of soil from having fungal growth via said bucket 105 and transferring said soil to a chamber 120 installed at ceiling portion of said housing 101, wherein said microcontroller activates a Peltier unit configured with said chamber 120 for maintaining an optimum temperature within said chamber 120 to allow growth of said fungi;
iv) a telescopically operated gripper 106 installed within said housing 101 that is actuated for gripping an agar plate 107 from a container 108 mounted within said housing 101 and placing said agar plate 107 in a receptacle 109 arranged within said housing 101, wherein said microcontroller actuates a first robotic arm 110 assembled within said housing 101 for transferring a small amount of said soil stored within said chamber 120 on said agar plate 107 via a spatula 111 equipped with said first robotic arm 110 followed by actuation of a motorized lid 112 configured with said receptacle 109 to close the opening for allowing growth of said fungi on said agar plate 107 for a pre-set time duration;
v) a second robotic arm 113 arranged within said housing 101 and equipped with an inoculating loop 114, wherein upon completion of said pre-set time duration, as monitored via a timer integrated within said microcontroller, said microcontroller actuates said lid 112 to open followed by actuation of said second robotic arm 113 for transferring a small amount of fungi from said agar plate 107 to a first flask 115 positioned on base of said housing 101 and stored with ferric chloride solution for catalyst test, such that an oxygen sensor arranged on mouth potion of said first flask 115 detects release of oxygen confirming presence of kojic acid extracting fungi;
vi) a platform 116 positioned within said housing 101 and placed with a flask-shaped body 117 stored with ethyl acetate, wherein upon confirming said presence of kojic acid extracting fungi, said microcontroller re-actuates said second robotic arm 113 for collecting fungi from said plate 107 and transferring said fungi to said body 117 for allowing growth of said fungi and extraction of kojic acid; and
vii) a second artificial intelligence-based imaging unit 118 paired with a processor mounted within said housing 101 for capturing and processing multiple images of said body 117, respectively, for detecting growth of fungi in said body 117, wherein upon detecting a threshold growth of said fungi, said microcontroller actuates said gripper 106 for placing said body 117 in a rotary evaporator unit 119 embedded within said housing 101, followed by activation of said rotator evaporator unit 119 for evaporating a solvent (ethyl acetate) from said body 117 and obtaining kojic acid in powdered form.
2) The device as claimed in claim 1, wherein upon activation of said rotary evaporator unit 119 for a threshold time limit, said microcontroller terminates working of said rotary evaporator unit 119, followed by sending an alert on said computing unit for notifying said user to collect said kojic acid from said body 117.
3) The device as claimed in claim 1, wherein said microcontroller is wirelessly linked with said computing unit via a communication module which includes, but not limited to Wi-Fi (Wireless Fidelity) module, Bluetooth module, GSM (Global System for Mobile Communication) module.
4) The device as claimed in claim 1, wherein said telescopically operated gripper 106 is powered by a pneumatic unit that includes an air compressor, air cylinder, air valves and piston which works in collaboration to aid in extension and retraction of said gripper 106.
5) The device as claimed in claim 1, wherein a battery is associated with said device for supplying power to electrical and electronically operated components associated with said device.
| # | Name | Date |
|---|---|---|
| 1 | 202421093870-STATEMENT OF UNDERTAKING (FORM 3) [29-11-2024(online)].pdf | 2024-11-29 |
| 2 | 202421093870-REQUEST FOR EXAMINATION (FORM-18) [29-11-2024(online)].pdf | 2024-11-29 |
| 3 | 202421093870-REQUEST FOR EARLY PUBLICATION(FORM-9) [29-11-2024(online)].pdf | 2024-11-29 |
| 4 | 202421093870-PROOF OF RIGHT [29-11-2024(online)].pdf | 2024-11-29 |
| 5 | 202421093870-POWER OF AUTHORITY [29-11-2024(online)].pdf | 2024-11-29 |
| 6 | 202421093870-FORM-9 [29-11-2024(online)].pdf | 2024-11-29 |
| 7 | 202421093870-FORM FOR SMALL ENTITY(FORM-28) [29-11-2024(online)].pdf | 2024-11-29 |
| 8 | 202421093870-FORM 18 [29-11-2024(online)].pdf | 2024-11-29 |
| 9 | 202421093870-FORM 1 [29-11-2024(online)].pdf | 2024-11-29 |
| 10 | 202421093870-FIGURE OF ABSTRACT [29-11-2024(online)].pdf | 2024-11-29 |
| 11 | 202421093870-EVIDENCE FOR REGISTRATION UNDER SSI(FORM-28) [29-11-2024(online)].pdf | 2024-11-29 |
| 12 | 202421093870-EVIDENCE FOR REGISTRATION UNDER SSI [29-11-2024(online)].pdf | 2024-11-29 |
| 13 | 202421093870-EDUCATIONAL INSTITUTION(S) [29-11-2024(online)].pdf | 2024-11-29 |
| 14 | 202421093870-DRAWINGS [29-11-2024(online)].pdf | 2024-11-29 |
| 15 | 202421093870-DECLARATION OF INVENTORSHIP (FORM 5) [29-11-2024(online)].pdf | 2024-11-29 |
| 16 | 202421093870-COMPLETE SPECIFICATION [29-11-2024(online)].pdf | 2024-11-29 |
| 17 | Abstract.jpg | 2024-12-23 |
| 18 | 202421093870-FORM-26 [03-06-2025(online)].pdf | 2025-06-03 |