Double Knock Out Cho Cell Line Method Of Its Generation And Producing

Double Knock Out Cho Cell Line Method Of Its Generation And Producing Therapeutic Proteins Therefrom

Abstract: The present disclosure relates to providing an engineered CHO cell line wherein the two essential metabolic genes are knocked out. Particularly, the present invention relates to a double knockout CHO cell line (DHFR-/- and GS-/-) with disrupted Dihydrofolate Reductase (DHFR) and Glutamine Synthetase (GS) genomic loci. The double knockout CHO cell line (DHFR-/- and GS-/-) being suitable for expression of monoclonal antibodies, dimeric therapeutic proteins, Fab, single chain fragments, or the like. The present disclosure also provides method of generation of a double knock out CHO cell line (DHFR-/- and GS-/-) using gene selection and/or manipulating techniques such as CRISPR/Cas9 system, Zinc Finger Nuclease, TALEN, or the like. The present disclosure further provides method of selection of clones and production of therapeutic proteins of interest with increased titre.

Patent Information

Application #

Filing Date

18 January 2021

Publication Number

05/2021

Publication Type

INA

Invention Field

BIOTECHNOLOGY

Status

Email

Parent Application

Applicants

ENZENE BIOSCIENCES LIMITED

Plot No. 165/1/26, Block T, Bhosari MIDC, Pune Maharashtra 411 026

Inventors

1. GADGIL, Himanshu

Plot No. 165/1/26, Block T, Bhosari MIDC, Pune Maharashtra 411 026

2. MOTIWALA, Hatim

Plot No. 165/1/26, Block T, Bhosari MIDC, Pune Maharashtra 411 026

3. CHAKRABORTY, Arindam

Plot No. 165/1/26, Block T, Bhosari MIDC, Pune Maharashtra 411 026

4. MULIK, Gayatri

ENZENE BIOSCIENCES LIMITED Plot No. 165/1/26, Block T, Bhosari MIDC Pune 411 026 Maharashtra

Specification

Documents

Application Documents

#	Name	Date
1	202147002278-FORM 18 [13-06-2022(online)].pdf	2022-06-13
1	202147002278-STATEMENT OF UNDERTAKING (FORM 3) [18-01-2021(online)].pdf	2021-01-18
2	202147002278.pdf	2021-10-18
2	202147002278-SEQUENCE LISTING(PDF) [18-01-2021(online)].pdf	2021-01-18
3	202147002278-SEQUENCE LISTING [18-01-2021(online)].txt	2021-01-18
3	202147002278-FORM 3 [28-06-2021(online)].pdf	2021-06-28
4	202147002278-FORM-26 [11-02-2021(online)].pdf	2021-02-11
4	202147002278-FORM 1 [18-01-2021(online)].pdf	2021-01-18
5	202147002278-DRAWINGS [18-01-2021(online)].pdf	2021-01-18
5	202147002278-Proof of Right [11-02-2021(online)].pdf	2021-02-11
6	202147002278-COMPLETE SPECIFICATION [18-01-2021(online)].pdf	2021-01-18
6	202147002278-DECLARATION OF INVENTORSHIP (FORM 5) [18-01-2021(online)].pdf	2021-01-18
7	202147002278-COMPLETE SPECIFICATION [18-01-2021(online)].pdf	2021-01-18
7	202147002278-DECLARATION OF INVENTORSHIP (FORM 5) [18-01-2021(online)].pdf	2021-01-18
8	202147002278-DRAWINGS [18-01-2021(online)].pdf	2021-01-18
8	202147002278-Proof of Right [11-02-2021(online)].pdf	2021-02-11
9	202147002278-FORM 1 [18-01-2021(online)].pdf	2021-01-18
9	202147002278-FORM-26 [11-02-2021(online)].pdf	2021-02-11
10	202147002278-SEQUENCE LISTING [18-01-2021(online)].txt	2021-01-18
10	202147002278-FORM 3 [28-06-2021(online)].pdf	2021-06-28
11	202147002278.pdf	2021-10-18
11	202147002278-SEQUENCE LISTING(PDF) [18-01-2021(online)].pdf	2021-01-18
12	202147002278-STATEMENT OF UNDERTAKING (FORM 3) [18-01-2021(online)].pdf	2021-01-18
12	202147002278-FORM 18 [13-06-2022(online)].pdf	2022-06-13