FIELD OF INVESIONS
It focuses on the extraction and characterization of plant-derived compounds,
particularly from Solanum nigrum, with an emphasis on discovering novel bioactive
molecules that may have therapeutic applications. This study contributes to the
growing body of knowledge on plant-based medicines and natural remedies. Natural
product chemistry is a primary focus, involving the isolation, purification, and
structural elucidation of bioactive compounds from natural sources, particularly
plants. Pharmacognosy plays a crucial role in examining the medicinal properties of
plants, including their phytochemical composition, bioactivity, and potential
therapeutic applications. Phytochemistry is also essential, investigating the chemical
composition of plants, including the identification and quantification of
phytochemicals .The research aims to advance knowledge on Solanum nigrum's
phytochemical profile and bioactivity, identifYing potential plant-based medicines and
natural remedies. It promotes sustainable and eco-friendly research practices,
enhancing understanding of structure-activity relationships in phytochemicals. This
study's findings will contribute significantly to the development of novel therapeutic
agents from plant-derived compounds.
Methodology :
• 5 grams of Solanum nigrum powder is used for the extraction.
• Powder is added to 50 mL of distilled water in a clean container.
• The mixture is stirred thoroughly to ensure complete suspension of the powder.
• The solution is filtered using an appropriate filtration apparatus to separate
undissolved solids.
• The filtrate is collected, and 10 mL of this liquid is set aside for further analysis.
• 90 mL ofKOH solution is prepared.
• The I 0 mL of filtrate is added to the KOH solution, resulting in a total volume
of 100 mL.
• The·mixture is placed on a magnetic stirrer and stirred until a noticeable color
change is observed .
• The color change indicates the interaction between bioactive compounds and
• Dilute acetic acid is added dropwise to the mixture while monitoring for a
subsequent color change.
• The colorimetric change further confirms the presence of nanoparticles.
• Additional analyses like UV-Vis spectroscopy, TEM, or SEM are recommended
for detailed characterization.
• These methods provide information on nanoparticle size and morphology.
• Colorimetric changes serve as qualitative indicators of nanoparticle formation in
the extract.
Claims:
I. The extraction method developed for Solanum nigrum using water as a solvent and
KOII as au exu·w;tion facilitator yields a significant concentration of bioactive
compounds.
2. According to claims I, The bioactive compounds obtained from Solanum nigrum
possess potential therapeutic properties, including antioxidant, anti-inflammatory, and
antimicrobial activities
3. According to claims 2, by continuing string, the extraction efficiency by ensuring
uniform mixing and improved interaction between the solvent and plant material.
4. According to claims 3 ,method provides a sustainable and cost-effective approach to
extract valuable phytochemicals from Solanum nigrum without the use of harmful
organic solvents .