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Fermented Milk Based Anxiolytic Agents

Abstract: The present disclosure relates to low molecular weight fermented milk-based products which demonstrate anxiolytic activity through the regulation of key proteins involved in mediating stress responses in mood disorders. Also provided are processes to obtain the same and uses thereof.

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Notices, Deadlines & Correspondence

Patent Information

Application #
Filing Date
28 April 2017
Publication Number
44/2018
Publication Type
INA
Invention Field
BIOTECHNOLOGY
Status
Email
iprdel@lakshmisri.com
Parent Application

Applicants

ITC LIMITED
ITC LIFE SCIENCES & TECHNOLOGY CENTRE #3. 1st Main, Peenya Industrial Area, Phase-1, Bangalore 560 058, India

Inventors

1. NAIR, Divya Radhakrishnan
ITC Limited, ITC Life Sciences & Technology Centre, No.3, 1st Main Road, Peenya Industrial Area, Phase I, Bengaluru 560 058, India
2. KALSI, Gurpreet
ITC Limited, ITC Life Sciences & Technology Centre, No.3, 1st Main Road, Peenya Industrial Area, Phase I, Bengaluru 560 058, India
3. CHANDRASEKHARAN, Lakshmanan Chittur
ITC Limited, ITC Life Sciences & Technology Centre, No.3, 1st Main Road, Peenya Industrial Area, Phase I, Bengaluru 560 058, India

Specification

Claims:1. A process for preparing a low molecular weight fermented milk product comprising:
(a) obtaining milk;
(b) adding a-lactalbumin and ß-lactoglobulin to said milk to obtain a first mixture;
(c) adding lactic acid bacteria to said first mixture to obtain a second mixture;
(d) incubating said second mixture to obtain a hydrolysate;
(e) centrifuging the hydrolysate to obtain a supernatant;
(f) filtering said supernatant to obtain said low molecular weight fermented milk product.
2. A process for preparing a low molecular weight fermented milk-based product comprising
(a) obtaining a solution comprising whey protein;
(b) adding a-lactalbumin and ß-lactoglobulin to the solution to obtain a first mixture;
(c) adding lactic acid bacteria to said first mixture to obtain a second mixture;
(d) incubating said second mixture to obtain a hydrolysate;
(e) centrifuging the hydrolysate to obtain a supernatant;
(f) filtering said supernatant to obtain said low molecular weight fermented milk-based product.
3. The process as claimed in claim 1, wherein said milk is obtained from a mammal.
4. The process as claimed in claim 2, wherein the solution comprising whey protein has a whey protein concentration in the range of 5-15%.
5. The process as claimed in claim 1 and 2, wherein a-lactalbumin concentration is in the range of 0.0005-0.002% and ß-lactoglobulin concentration is in the range of 0.003-0.007%.
6. The process as claimed in claim 1 and 2, wherein lactic acid bacteria concentration is in the range of 5x107 CFU/ml-12x107 CFU/ml.
7. The process as claimed in claim 1 and 2, wherein lactic acid bacteria are selected from the group consisting of Lactobacillus casei, L. helveticus, L. delbrueckii, L. acidophilus, Lactococcus lactis, and combinations thereof.
8. The process as claimed in claim 1 and 2, wherein incubating the second mixture is at a temperature in the range of 28oC- 40 ? for a time period in the range of 6-24 hours.
9. The process as claimed in claim 1 and 2, wherein said hydrolysate is centrifuged in the range of 8000- 12000rpm for 10- 20 minutes.
10. The process as claimed in claim 1 and 2, wherein said supernatant is filtered using a 3 kDa molecular weight filter to obtain said low molecular weight fermented milk product.
11. The process as claimed in claim 1 and 2, wherein said low molecular weight fermented milk product has a peptide content in the range of 0.05 mg/ml to 0.2 mg/ml.
12. A low molecular weight fermented milk-based product having a peptide content in the range of 0.05 mg/ml to 0.2 mg/ml.
13. The low molecular weight fermented milk-based product as claimed in claim 12, wherein the product is obtained from the process as claimed in any of the claims 1 and 2.
14. The low molecular weight fermented milk-based product as claimed in claim 12, wherein said product alleviates anxiety and mood related disorders.

15. The low molecular weight fermented milk-based product as claimed in claim 12, wherein said product increases brain-derived neurotropic factor.
, Description:AS ATTACHED

Documents

Application Documents

# Name Date
1 Form 5 [28-04-2017(online)].pdf 2017-04-28
2 Form 3 [28-04-2017(online)].pdf 2017-04-28
3 Drawing [28-04-2017(online)].pdf 2017-04-28
4 Description(Complete) [28-04-2017(online)].pdf_196.pdf 2017-04-28
5 Description(Complete) [28-04-2017(online)].pdf 2017-04-28
6 Other Patent Document [22-05-2017(online)].pdf 2017-05-22
7 Form 26 [22-05-2017(online)].pdf 2017-05-22
8 Correspondence by Agent_Proof Of Right_24-05-2017.pdf 2017-05-24
9 IMPORTANT LETTER FROM NBA.pdf 2019-03-19
10 201741015212-FORM 18 [03-02-2022(online)].pdf 2022-02-03