FIELD OF THE INVENTION:
The invention discloses a kind of anti-aging cream is grown. This application discloses an
anti-aging composition from extract of different fruits. The composition is formulated with
other components to serve as a cleanser, a moisturizer, a serum, a gel masque, an
eye cream, a toner, or an exfoliant.
BACKGROUND OF THE INVENTION:
The present invention relates· to cosmetic products, namely, anti-aging compositions for
dermal application. The composition comprises various fruit extracts and other material.
The anti-aging composition can be applied as a serum or moisturiser to hydrate dry skin, as
an exfoliant to remove ·dead skin cells, as a gel masque to massage and soothe ageing skin
cells.
In the Present Invention, Skin's oxidative stress may be decreased with the use of topical
treatments. The fruit waste known as Fruits peel has antioxidant properties but has not yet
been incorporated into a topical preparation.
The present Invention's objective was to assess the antioxidant and anti-aging properties of
a cream formulation comprising lyophilized Fruits peel extract. A cream formulation
comprising 1% of the extracted Fruits peel was created after the peel was removed using
ethanol. Both the extract and the formulation were examined for their total phenolic
content (TPC), total flavonoid content (TFC), antioxidant, and anti-aging activity. For an
antioxidant effect, FRAP (Iron ion reduction), CUPRAC (copper ion reduction), ABTS (2, 2'Azinobis
{3-ethylbenzotrazole-6-sulfonate), and DPPH · {2, 2-Diphenyl-1-picrylhydrazyl)
techniques were utilised. Elastase enzyme inhibitory activity was investigated for its antiaging
properties. Comparatively to the norms, the Fruits peel extract demonstrated a high
antioxidant and anti-aging impact. Even though the extract was only added 1% to the cream,
it still had roughly 80% of the extract's antioxidant and anti-aging benefits. TPC and TFC
results also demonstrated that phenolic and flavonoid chemicals were responsible for this
outcome. One could argue that this formulation has the potential to be an antioxidant and
anti-aging agent when applied topically.
Summary of the invention:
The present invention .relates to a Skin aging is a complex process induced by constant
exposure to ultraviolet (UV) irradiation and damages human skin. Melanogenesis is the
complex process which is responsible for the formation of pigment melanins by
2
melanocytes while skin wrinkling is associated with collagen deficiency resulting from
reactive oxygen species that is generated from UVl . Skin aging is associated with many
factors and when doing researches on the topic, one will definitely come across to terms like
ROS (reactive oxygen species) which generally a free radical that contributes to skin aging.
ROS is basically generated during ·cellular metabolism, but the excessive production of ROS
can lead to OS (oxidative stress). OS can be a precursor to a lot of health damaging issues
including skin aging. The fruits are used for treating various diseases like hypertension,
kidney disorder, cancer and diabetes.
The present invention provides that some beneficial bioactivities of this fruits anti-bacterial,
anti-hyperglycemic and antihyperlipidemia. Different Fruits having antidiabetic, anticancer,
antioxidant, antiinflammatory, antiviral and antimicrobial activities.
·The present invention also provides for a method that includes Equal pigmentation,
increasing wrinkles, elasticity loss, dryness,. and roughness are all signs of ageing skin.
Mahkota Dewa is the Malay name for Phaleria macrocarpa, which is a popular plant in
Malaysia. Fruits, which is abundant in the fruits and has therapeutic qualities including antidiabetic,
antioxidant, anti-proliferative, immunodulatory, cardiotonic, and diuretic effects, is
present in high concentrations. However, no formulation has been developed.
The present invention also provides for a method that includes even though various
biological features had been described for "it. Our study's objective is to create and assess an
anti-aging cream with Fruits as an active component. By combining oil and aqueous phase
with the use of a homogenizer, two formulations with various amounts of Fruits were
created. Significant antioxidant activity and tyrosinase inhibition were ·reported in both
formulations. The creams were developed with consistency in quality and were secure for
usage on the skin. These findings show that the isolated Fruits from ·P. macrocarpa has a
promising future as a cosmetic ingredient.
3
Objectives of the present invention:
It is a primary object Of the present invention to provide a pharmaceutical composition for
controlled release of an erythromycin derivative.
It is a primary object of the present invention to provide to assess the antioxidant and antiaging
properties of a cream formulation comprising lyophilized Fruits peel extract. A cream
formulation comprising 1% of the extracted Fruits peel was created after. the peel was
removed using etha·nol. Both the extract and the formulation were examined for their total
phenolic content (TPC}, total flavonoid content (TFC}, antioxidant, and anti-aging activity.
For an antioxidant effect, FRAP (Iron ion reduction), CUPRAC (copper ion reduction), ABTS
(2, 2'-Azinobis (3-ethylbenzotrazole-6-sulfonate), and DPPH {2, 2-Diphenyl-1-picrylhydrazyl)
techniques were utilised. Elastase enzyme inhibitory activity was investigated for its antiaging
properties. Comparatively to the norms, the Fruits peel extract demonstrated a high
antioxidant and anti-aging impact. Even though the extract was only added 1% to the cream,
it still had roughly 80% of the extract's antioxidant and anti-aging benefits. TPC and TFC
results also demonstrated that phenolic and flavonoid chemicals were responsible for this
outcome. One could argue that this formulation has the potential to be an antioxidant and
anti-aging agent when applied topically.
The first-object of the present invention is to provide a kind of anti-aging cream and grows,
and removes free radical, strengthens body function, moistens skin Skin, toxin-expelling and
face nourishing.
The second object of the present invention is to provide a kind of method for preparing that
anti-aging cream is grown.
It is the further objective of the present invention to method of preparation of the
controlled release formulation.
Description of the invention:
The most frequent oral paste dosage type used in India is called cream, sometimes known
as soft extract. It is based on simultaneous nourishing and is controlled carefully. The
· patient enjoys using the treatment since it is nice and pleasant and contains honey.
Topical formulations may contribute to the reduction of oxidative stress in the skin. Fruits. peel is a
fruit waste with antioxidant activity that is not yet included in a topical formulation .. The aim of this
study was to evaluate the antioxidant and anti-aging activity of a cream formulation containing a
4
lyophilized extract of Fruits peel. The Fruits peel was extracted with ethanol, and a cream
formulation containing 1% of the resulting extract was prepared. The total phenolic content (TPC),
total flavonoid content (TFC), antioxidant and anti-aging activity of both extract and formulation
were investigated. DPPH (2, 2-Diphenyl-1-picrylhydrazyl) free radical scavenging, ABTS (2, 2'-Azinobis
(3-ethylbenzotrazole-6-sulfonate) cation radical scavenging, FRAP (Iron ion reduction) and CUPRAC
(copper ion reduction) methods were used for antioxidant effect. For the anti-aging effect, elastase
enzyme inhibitory activity was studied. The Fruits peel extract had a high antioxidant and anti-aging
effect compared to the standards. Although the extract was added 1% in the cream, the cream
showed antioxidant and anti-aging effects of about 80% of the extract. TPC and TFC findings also
showed that this result due to phenolic and flavonoid compounds. It is possible to suggest that this
formulation has the potential of antioxidant and anti-aging for topical use.
In addition to providing nourishment, health care, and salubrity, cream develop therapy has
anti-aging properties that help people live longer. a sugar with a beneficial function, honey
more so, can boost the body's immunity; From a formulation perspective, cream is
produced and accepts juice concentrates. The best medicine, with little of it and pure
material that is simple to assimilate; Additionally, utilizing a simple, comfortable method
that doesn't require boiling medicine every day, has a sweet, happy tongue, and is simple to
apply for a longer period of time; Consequently, the therapy for chronic kidney disease and
the body's recovery following illness, postpartum, are particularly appropriate.
The present invention can take on a number of different forms. The following description of
various embodiments is provided with the understanding that it should only be used as an
exemplification of the subject matter covered by the attached claims and is not meant to
impose any restrictions on those claims. The headings used throughout this disclosure are
just included for convenience and should not be taken as any kind of restriction on the
claims. Any combination of the embodiments shown under any heading with those shown
under. any other heading is permitted.
Preparation of Extract: Fruits peels were crushed in a lab blender to prepare the extract,
which was then extracted in ethanol for a week at room temperature. Three times a week,
the solvent was replaced. A rotary evaporator was then used to eliminate the solvent. Until·
the investigation, the extracts were kept at 4 ·c.
5
Evaluation of Prepared Cream Formulations: Physical Evaluation: The banana peel cream
was tested for odour, appearance, and homogeneity through visual observation and touch.
Determination of Ph: The pH meter was calibrated using standard buffer solutions. About
0.5 g of the cream was weighed and dissolved in 50 ml of distilled water in a beaker, and its
pH was measured.
Viscosity: The measurement of viscosity of the prepared cream from banana peel was done
with a Brookfield viscometer. The reading was taken at 100 rpm using spindle no. 6.
Spreadability: Emolliency, slipperiness, and amount of residue left after the application of
the cream was checked. Thermal Stability: Stability studies were carried out as per ICH
guidelines for the formulated cream to access it~ stability parameters during its storage
period. The cream-filled bottle was kept in a humidity chamber maintained 35 + 2 •c with
65+5% relative humidity (RH) for two months. At the end of the studies, samples were
analyzed for their physical properties.
Microbiological Test: The formulated creams were tested for their-sterility. A small quantity
of the cream was inoculated into Muller Hilton agar media through the streak plate method
and was at 37 ·c for 24 h. After the incubation period, the plates were checked for any
microbial growth by_ comparing with the control plate.
Determination of Total Phenolic Content: The amount of phenolic compounds found in the
extract prepared from the banana peels and the cream prepared using this extract was
determined by Falin Ciocalteu method 27, 28 . Gallic acid was used as the standard phenolic
compound. A calibration graph of the gallic acid was plotted. 1000 ppm gallic acid solution
was prepared. 0, 1, 2, 3, 4, 5, 6, 7 and 8 Ill of this solution were added to the microplate
wells .and their volumes were completed to 184 Ill with distilled water. Extract solutions
were prepared at concentrations of 1-20 mg/ml, and cream solutions were also prepared at
concentrations of 20-80 mg/ml. 4 Ill of prepared samples were added to microplate wells,
and these solutions. were completed with 184 Ill distilled water. 4 ml of Folin-Ciocalteu
reagent and after 3 min, 12 ml of 2% Na2C03 solution was added to the gallic acid solutions
and samples. Distilled water was used instead of the sample for control. The solutions were
6
incubated for 2 h, and the absorbances of them were read at 760 nm. Results were
calculated as microgram in gallic acid equivalent (GAE).
Determination of Total Flavonoid Content: The amount of flavonoid compounds found in
the extract prepared from the. banana peels and the cream prepared using this extract was
determined by aluminum nitrate method 29 . Quercetin was used as the standard flavonoid
compound. A calibration graph of the quercetin was plotted. 1000 ppm quercetin solution
was prepared. 0, 1, 2, 3, 4, 5, 6, 7, and 8 Ill of this solution were added to the microplate
wells, and their volumes were completed to 192 Ill with 80% ethanol. 4 Ill of potassium
acetate, and after a minute, 4 Ill of 10% aluminum nitrate was added to the microplate
wells containing quersetin solution and samples. Distilled water was used instead of the
sample for control. The solutions were incubated for 40 minutes, and the absorbances of
them were read at 415 nm. Results were calculated as microgram in quercetin equivalent
(QE).
Determination of Antioxidant Activity: DPPH Free Radical Scavenging Activity: DPPH free
radical scavenging activities of the extracts prepared from banana peels and the creams
prepared from these extracts were applied according to the Blois method 30. 0.1 mM
solution of DPPH was used as free radical. 1-10 mg/ml stock solutions of extract and 20-80
nig/ml stock solutions of cream were prepared with ethanol. 2- 20 Ill of the stock solution
was added to the wells and completed to 40 Ill with ethanol. Then, 160 Ill of DPPH solution
was added to the mixtures in the wells. The final mixtures were incubated for 30 min. The
absorbances of them at 517 nm were read. Ethanol was used as the control.
ABTS Cation Radical Scavenging Activity: ABTS cation radical scavenging activities of the
extracts prepared from banana peels and the creams prepared from these extracts were
applied according to Re et al. 31. 1-10 mg/ml stock solutions of extract and 20-80 mg/ml
stock solutions of cream were prepared with ethanol. 2- 20 Ill of the stock solutions were
added to the wells and completed to 40 Ill with ethanol. Then, 160 Ill of ABTS solution.was
added to the mixtures in the wells. The final mixtures were incubated for 30 min. The
absorbances of them at 734 nm were read. Ethanol was used as the control.
Cupric lon Reducing Antioxidant Capacity (CUPRAC): Copper II ion reducing antioxidant
capacity of the extracts prepared from banana peels and the creams prepared from these
7
extracts were applieq according to the Apak et al. 32.1-10 mg/ml stock solutions of extract
and 20-80 mg/ml stock solutions of cream were prepared with ethanol. 2- 20 Ill of the
stock solution was added to the wells and completed to 67 Ill with ethanol. Then, 61 Ill of
0.01 M CuCI2 solution, 611-ll of 7.S x10-3 M neocuproihe solution, and 611-ll of 1M acetate
buffer were added to the mixtures in the wells. The final mixtures were incubated for 30
min. The absorbances of them at 4SO nm were read. Ethanol was used as the control.
Ferric Reducing Antioxidant Power (FRAP): Iron Ill ion reducing antioxidant power of the
extracts prepared from banana peels and the creams prepared from these extracts were
applied according to the Benzie and Strain 33 . 1-10 mg/ml stock solutions of extract and .
20-80 mg/ml stock solutions of cream were prepared with ethanol. FRAP reagent was
freshly prepared before use. 10 mM 2, 4, 6-Tris (2-pyridyl)- s- triazine (TPTZ) is· dissolved in
40 mMHCI. 20 mM FeCI3 solution and 300 mM pH 3.6 acetate buffer were prepared with
distilled wate"r. The reagent was prepared by mixing TPTZ, FeCI3, and acetate buffer in a
ratio of 1:1:10. 2-20 Ill of the stock solution was added to the wells and completed to 40 Ill
with ethanol. Then, 240 Ill of FRAP reagent was added to the mixtures in the wells. The final
mixtures were incubated for 30 min. The absorbances of them at S93 nm were read.
Ethanol was used as the control.
Anti-aging Activity Assay: Elastase Inhibitory Activity: Elastase enzyme inhibitory potential
of the extracts prepared from banana peels and the creams prepared from these extracts
were applied according to EnzChek"' Elastase Assay Kit (E-120S6) procedure 34. 1-10 mg/ml
stock solutions of extract and 20-80 mg/ml stock solutions of cream were prepared with
DMSO. 2-20 Ill of the stock solution was added to the wells and completed to SO Ill with
DMSO. Then, SO lllof 100 llg/ml Elastin and SO Ill of elastase enzyme were added to the
mixtures in the wells. The final mixtures were incubated for 30 minutes. The absorbances of
them at S93 nm were read. N-methoxysuccinyi-Aia -Ala - Pro- Valchloromethyl ketone was
used as the positive control, and reaction buffer was used as the negative control.
Evaluation of Cream Formulation: While preparing, the cream was evaluated according to
various parameters. The results are seen in Table of the cream was detected to between 6.8
and 7.1. The cream was seen to have an odour similar to the banana aroma in an acceptable
8
level and color like banana yellow. When applied, the cream was determined to have a
moisturizing and slippery, nice feeling on the skin. It was found that the cream protected its
physical parameters,. namely, kept its stability during the study, and any microbial organism
wasn't created in the cream.
TABLE: EVALUATION PARAMETER OF CREAM PREPARED USING BANANA PEEL EXTRACT
Parameter Observation
Odour Acceptable
Appearance Banana yellow
Homogenity Homogenous
pH 6.8-7.1
Viscosity 4600cp
Spreadability Good
After feel Emollients and slipperiness
Stability Stable until the entire period of study
Microbial test No growth of colonies
Odour Acceptable Appearance Banana yeUow Homogenity Homogenous pH 6.8-7.1
Viscosity 4600 cp Spreadability Good After feel Emollients and slipperiness Stability. Stable
until the entire period of study Microbial test No growth of colonies
Fruit, a tropical fruit, is.consumed as a nutritious fruit for a long year. Its peel is tried to be
proved that might have various biological impacts. Fruit peel extract and cream formulation
prepared from it showed a high level of antioxidant and elastase inhibition activity. It was
detected that the cream which was formulated according to the findings might have anti·
aging potential and can be used on the skin. It was found that adding banana peel extract
into the cream at the rate of 1% in 20 mg/mL concentration can be used as anti-aging,
according to the results. Using Fruit peel in cosmetics products was researched in the study,
and other researches for skin will be conducted in further studies.
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We claim:
1. A method for treating the skin of a subject in need thereof, the method comprising
topically applying to the skin of the subject a composition that includes an effective
amount of an extract of fruits, a dermal topical composition for treating human skin
which has anti ageing, is sagging or has pigmentation due to age of the human
consisting essentially of:
(a) a dermatologically amount of cannabis sativa L oil having a cannabinoid content
of at least 10% (wt/wt), at between about 0.1% (wt/wt) and ab9ut 5% (wt/wt) of the
composition;
(b) about 0.1% to 2% of Angelica sinensis extract;
about 0.1% to 3.5% Croton lechleri resin;
about 01% to 2% of Astragalus membranaceous Root extract; and
2. The composition of claim 1, further consisting essentially of oil at between about 1%
(wt/wt) and about 3% (wt/wt)
3. The composition of claim 1, further consisting essentially of a sufficient quantity of
herbal chemicals to bring the pH of the composition to between 6.8 and 8.
4. The composition of claim 1, further consisting essentially of water with up to 100%
of the weight of the composition being water.
5. The composition of claim 1, further consisting essentially of glycerine at between
about 1% (wt/wt) and about 8% (wt/wt).