DESC:Field of the invention
This invention relates to the sustainable agriculture and involved the effect of molasses on the growth of okra for eco-friendly farming and economy of country.
Background of the Invention
References which are cited in the present disclosure are not necessarily prior art and therefore their citation does not constitute an admission that such references are prior art in any jurisdiction. All publications, patents and patent applications herein are incorporated by reference to the same extent as if each individual or patent application was specifically and individually indicated to be incorporated by reference.
Several patents have been issued for the plant growth. For example, US20180020676A1 provides compositions and methods include a new strain of Bacillus velezensis having growth promoting activity and activity against plant pathogens. The compositions are useful for benefiting plant growth and/or conferring protection against a pathogenic infection when applied to plant foliage, flowers, fruits, bark, roots, seeds, callus tissue, grafts, cuttings, surrounding soil or growth medium, and soil or growth medium concomitant with sowing seed and planting callus tissue, grafts, and cuttings. The compositions containing the Bacillus velezensis RTI301 strain can be applied alone or in combination with other microbial, biological, or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, plant growth regulators, and fertilizers. In one example, the Bacillus velezensis RTI301 strain can be delivered to the plant as part of an integrated pest management program, with other microbial or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, and plant growth regulators.
Another patent, US10212943B2 relates to the field of plant-microorganism interactions. In particular, the present disclosure relates to methods and compositions for increasing plant growth characteristics by growing the plant in the presence of plant growth-promoting microbial strains.
Another patent, US11040920B2 provides A slow release carbohydrate/bicarbonate/nitrogen fertilizer which includes: a nitrogen component selected from the group consisting of a urea compound, ammonium bicarbonate, ammonium sulfate and ammonium nitrate; a bicarbonate component selected from the group consisting of ammonium bicarbonate, potassium bicarbonate and sodium bicarbonate; and a soluble carbohydrate component selected from the group consisting of a starch and a sugar, wherein the fertilizer is applied to increase crop yield and increase protein levels in plant products by placing the aforesaid fertilizer beneath the surface of soil on which is growing cultivated plants.
Another patent, US10588320B2 comprises compositions and methods for biostimulation, such as seed germination and plant growth. For example, the present disclosure comprises compositions and methods for affecting, enhancing, modulating, stimulating, or aiding plant growth. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
Another patent, JP6758296B2 compositions and methods are provided for a combination of a new strain of Bacillus amyloliquefaciens RTI301 and a new strain of Bacillus subtilis RTI477, the combination having growth promoting activity and activity against plant pathogens. The compositions containing the RTI301 and RTI477 strains are useful for benefiting plant growth and/or conferring protection against a pathogenic infection when applied to plant roots, seeds, callus tissue, grafts, and cuttings. Synergistic results are observed for the combination of the strains, and the combination of strains is useful to increase yield in crops including soybean and corn. The compositions containing the combination of strains can be applied alone or in combination with other microbial, biological, or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, plant growth regulators, and fertilizers.
Okra is a multipurpose crop due to the varied use of its leaves, buds, flowers, pods, stems, and seeds as well. The three main planting seasons for Okra are February-April, June-July and October-November and can be grown almost in every part of the country. Soil should ideally be on the acidic side, with a pH between 5.8 and?7.0. It is a tropical direct sown vegetable with a duration of 90-100 days. Previously, the extract from seeds of lady finger was demonstrated to contain polyphenols, tannin, flavonoids, terpenoids, saponins, long chain fatty acids, and glutathione. Okra has long been used as a regular vegetable and a source of dietary medicine to cure several serious diseases along with. Potential beneficial effects associated to okra and their components are cardioprotective, antidiabetic, renal protective, neuroprotective, anticancer, analgesic, antiulcer, antibacterial, and antifatigue. The industrial urbanization discharges wastes to nearby land and river that in turn affects qualities and quantities in food crops as well. Molasses produced annually in large quantities and used in various industries for production of animal feed, alcohol and fertilizers. The use of sugar beet molasses in agriculture stimulates nutrient elements uptake efficiency and soil biological activity. The physicochemical analysis of diluted molasses showed that it is slightly acidic and contains a fairly good amount of calcium, magnesium and other essential nutrients like sodium potassium, chlorides, carbonates, bicarbonates and sulphates. Filter mud cake (FMC), Farm yard manure (FYM) and molasses increased NPK (N, Nitrogen; P, Phosphorus; K, Potassium) uptake and yields by improving soil aggregation and reduces surface crusting in hard-setting soils. Hence, molasses as organic fertilizer could be substitute to it for better growth and production of crops. Therefore, the present patent is designed to accelerate the okra growth by using molasses as biodegradable formulation technique and enhance its productivity in both qualitative and quantitative terms for eco-friendly farming and sustainable agriculture.
The primary object of the present invention is the formulation of molasses as growth supplement to okra.
The primary object of the present invention is to design to accelerate the okra growth by using molasses as biodegradable formulation technique and enhance its productivity in both qualitative and quantitative terms for eco-friendly farming and sustainable agriculture.
Another object of the present invention is to overcome the impacts of chemical fertilizers and effluents on cropping and health, the application of formulation molasses as organic fertilizer was prescribed by the present innovation.
Another object of the present invention is the use of diluted molasses or blending of molasses to cropland soils in agriculture may save the expenses of fertilizers and augments the economy of farmers whenever utilized in standard proportion.
Another object of the present invention is to use the molasses as profound organic fertilizer for eco-friendly farming and sustainable agriculture.
These and other objects and advantages of the present invention will become readily apparent from the following detailed description.
Summary of Invention
This summary is not a comprehensive overview of the disclosure and does not reflect the main/essential features of the establishment or specify the scope of the establishment. Its sole purpose is to present some of the concepts presented here in a simpler way as a precursor to more detailed explanations presented later.
The present invention relates to formulation of molasses as growth supplement to okra.
In some embodiments of the present invention, Okra (Abelmoschus esculentus L.) is the vegetable crop with high-value in terms of nutrients important to human health and comprising vitamins, potassium, calcium, carbohydrates, dietary fiber, unsaturated fatty acids and bioactive chemicals.
In some embodiments of the present invention, the plants may also showing pharmacological potential and to be known for cure of various serious diseases because of due to antidiabetic, cell reinforcement, anti-adhesive, gastro-protective, hepato-protective, anti-cancer and immuno-modulating activities.
In some embodiment of this aspect of the present invention, to overcome the impacts of chemical fertilizers and effluents on cropping and health, the application of formulation molasses as organic fertilizer was prescribed by the present patentable innovation.
In some embodiment of this aspect of the present invention, okra plants were sown in Botanical garden soils blended with different percentages of molasses (w/w) viz. 5%, 3%, 1% and 0% (control group).
In some embodiment of this aspect of the present invention, soil blended with 5% molasses (w/w) gave profound positive results than the other concentrations and control group as well.
These and other aspects of the embodiments herein will be better appreciated and understood when considered in concurrence with the following explanation and the accompanying drawings. It should be understood, however, that the following descriptions, while indicating preferred embodiments and numerous specific details thereof, are given by way of illustration and not of limitation. Many changes and modifications may be made within the scope of the embodiments herein without departing from the spirit thereof, and the embodiments herein include all such modifications.
Brief summary of the drawings
Fig. 1: Total height of okra plant in experimental and controlled groups at different interval. Where: I, 5th March 2020; II, 23rd March 2020; III, 30th March, 2020; IV, 7th April 2020.
Fig. 2: Total number of leaves and fruits of okra in experimental and controlled groups on 7th April, 2020.
Brief summary of the tables
Table 1: Growth attributes (Abelmoschus esculentus (L.) Moench) in experimental groups (with molasses) and controlled group (without molasses) at different interval.
These embodiments are described in sufficient detail to enable those skilled in the art to practice the embodiments and all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
The present invention relates to formulation of molasses as growth supplement to okra.
In some embodiments of the present invention, okra plants were sown in Botanical garden soils blended with different percentages of molasses (w/w) viz. 5%, 3%, 1% and 0% (control group). The different attributes of growth of plants were regularly monitored.
In some embodiments of the present invention, soil blended with 5% molasses (w/w) gave profound positive results than the other concentrations and control group as well. That implies supplements present in diluted molasses are fundamental at a specific concentration and consequently compassionate to the plant development.
In some embodiments of the present invention, four soil pots were prepared in the Botanical garden blended with different percentages of molasses (w/w) viz. 5%, 3%, 1% and 0% (control).
In some embodiments of the present invention, the maximum plant growth 13cm (Total height 35cm) was recorded in soil pot with 5% molasses followed by 10cm (Total height 30.5cm) in soil pot with 1% molasses.
In some embodiments of the present invention, the soil pot without blending of molasses (controlled group) showed even equal rate of germination but the growth rate was minimum with 5cm height only (Total height 14cm).
In some embodiments of the present invention, the maximum number of leaves 10 were observed in P1 (5% molasses) and minimum number of leaves 3 in P3 soil pot with 1% molasses (Figure 2). The fruits were observed maximum 15 in P1 (5% molasses) followed 10, 8, and 6 fruits or green seed pods in P4 (0% molasses), P2 (3% molasses) and P3 (1% molasses) respectively.
A formulation of molasses as growth supplement to okra consists of steps:-
a. preparing four soil pots in the Botanical garden blended with different percentages of molasses (w/w) viz. 5%, 3%, 1% and 0% (control);
b. irrigation of all sets equally is done with water at same interval; and
c. recording the growth attributes viz. number of seeds germinated, shoot height, number of leaves and number of fruits on each plant after the given time interval.
The maximum plant growth 13cm (Total height 35cm) is recorded in soil pot with 5% molasses.
The maximum plant growth 10cm (Total height 30.5cm) is recorded in soil pot with 1% molasses.
The soil pot without blending of molasses (controlled group) showed even equal rate of germination but the growth rate was minimum with 5cm height only (Total height 14cm).
The maximum number of leaves 10 are observed in P1 (5% molasses) and minimum number of leaves 3 in P3 soil pot with 1% molasses.
The fruits are observed maximum 15 in P1 (5% molasses) followed 10, 8, and 6 fruits or green seed pods in P4 (0% molasses), P2 (3% molasses) and P3 (1% molasses).
EXAMPLES
Experimental
Molasses or black treacle is a viscous by product during refining sugarcane or sugar beets into sugar which contains a concentrated level of the nutrients and minerals that were available in the actual sugarcane. In the present investigation, okra plants were sown in Botanical garden soils blended with different percentages of molasses (w/w) viz. 5%, 3%, 1% and 0% (control group). The different attributes of growth of plants were regularly monitored. The present work showed that, soil blended with 5% molasses (w/w) gave profound positive results than the other concentrations and control group as well. That implies supplements present in diluted molasses are fundamental at a specific concentration and consequently compassionate to the plant development. Therefore, the use of diluted molasses or blending of molasses to cropland soils in agriculture may save the expenses of fertilizers and augments the economy of farmers whenever utilized in standard proportion. Therefore, it is recommended the molasses as profound organic fertilizer for eco-friendly farming and sustainable agriculture.
Experimental Design
The seeds of okra (Abelmoschus esculentus (L.) Moench) were procured from the Numberdar Trading Company, New Grain Market, Shahabad- Markanda, Ambala (HR), India. The seeds were maintained in the Botany Laboratory, Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala (HR), India. Four soil pots were prepared in the Botanical garden blended with different percentages of molasses (w/w) viz. 5%, 3%, 1% and 0% (control) after Thakare et al. (2013). Two seeds were sown in the experimental and control soil pots of Botanical garden on 25th February, 2020. All sets were irrigated equally with water at same interval. The growth attributes viz. number of seeds germinated, shoot height, number of leaves and number of fruits on each plant were recorded after the given time interval
EXAMPLE 2
Results and Discussion
All the growth attributes (number of seed germinated, height of plants, number of leaves in each plant and number of fruits) in experimental and control groups were periodically assessed and recorded (Table 1). The maximum plant growth 13cm (Total height 35cm) was recorded in soil pot with 5% molasses followed by 10cm (Total height 30.5cm) in soil pot with 1% molasses (Figure 1). The soil pot without blending of molasses (controlled group) showed even equal rate of germination but the growth rate was minimum with 5cm height only (Total height 14cm). The maximum number of leaves 10 were observed in P1 (5% molasses) and minimum number of leaves 3 in P3 soil pot with 1% molasses (Figure 2). The fruits were observed maximum 15 in P1 (5% molasses) followed 10, 8, and 6 fruits or green seed pods in P4 (0% molasses), P2 (3% molasses) and P3 (1% molasses) respectively (Figure 2).
Table 1: Growth attributes (Abelmoschus esculentus (L.) Moench) in experimental groups (with molasses) and controlled group (without molasses) at different interval.
Parameters Experimental group (5% molasses) P1 Experimental group (3% molasses) P2 Experimental group (1% molasses) P3 Controlled group (0% molasses) P4
Time interval I II III IV I II III IV I II III IV I II III IV
Shoot growth (cm) 4 6 12 13 3.5 4 6 7 6.5 6 8 10 2 3 4 5
Total number of leaves 2 4 6 10 2 3 4 4 2 1 3 3 2 2 3 4
Total number of fruits - - 8 15 - - 4 8 - - 2 6 - - 1 10
Where: I, 5th March 2020; II, 23rd March 2020; III, 30th March, 2020; IV, 7th April 2020.

During the investigation it has been revealed that molasses concentrations 5% and 3% were favorable for significant growth of okra plant, number of leaves and fruits. These findings were in conformity with the observations recorded in jowar, bajra and rice. At these blending concentrations the ladies’ finger plants were efficiently able to absorb maximum nutrients both from soil and diluted molasses resulted augmented yields. This indicates that, the ingredients present in the blends of diluted molasses and soil at particular concentration were supportive to the growth and production of plants. The molasses as organic fertilizer supplied carbohydrates and alters C:N ratio (carbon : nitrogen ratio) which affects soil microbial ecology and lowers plant parasitic nematodes as well as provided other favourable environment for better plant growth. It was reported that, due to oxidation of carbohydrates (60-70% molasses) organic acids were formed which dissolves the native calcium carbonate and helped in reclamation of alkali soils Dhar. Molasses also increases the growth of shoot length, leaf number per plant, leaf area and chlorophyll content of peas.
,CLAIMS:
1. A formulation of molasses as growth supplement to okra consists of steps:-
a. preparing four soil pots in the Botanical garden blended with different percentages of molasses (w/w) viz. 5%, 3%, 1% and 0% (control);
b. irrigation of all sets equally is done with water at same interval; and
c. recording the growth attributes viz. number of seeds germinated, shoot height, number of leaves and number of fruits on each plant after the given time interval.
2. The formulation of molasses as claimed in claim 1, wherein the maximum plant growth 13cm (Total height 35cm) is recorded in soil pot with 5% molasses.
3. The formulation of molasses as claimed in claim 1, wherein the maximum plant growth 10cm (Total height 30.5cm) is recorded in soil pot with 1% molasses.
4. The formulation of molasses as claimed in claim 1, wherein the soil pot without blending of molasses (controlled group) showed even equal rate of germination but the growth rate was minimum with 5cm height only (Total height 14cm).
5. The formulation of molasses as claimed in claim 1, wherein the maximum number of leaves 10 are observed in P1 (5% molasses) and minimum number of leaves 3 in P3 soil pot with 1% molasses.
6. The formulation of molasses as claimed in claim 1, wherein the fruits are observed maximum 15 in P1 (5% molasses) followed 10, 8, and 6 fruits or green seed pods in P4 (0% molasses), P2 (3% molasses) and P3 (1% molasses).