DESC:FORM 2

THE PATENTS ACT, 1970
(39 of 1970)

As amended by the Patents (Amendment) Act, 2005
&
The Patents Rules, 2003

As amended by the Patents (Amendment) Rules, 2016

COMPLETE SPECIFICATION
(See section 10 and rule 13)

TITLE OF THE INVENTION
FUNGICIDAL COMPOSITION

APPLICANT
INDOFIL INDUSTRIES LIMITED, an Indian company of Kalpataru Square - 4th floor, Kondivita Road, Off Andheri Kurla Road, Andheri (East), Mumbai 400059, India,
and
NIPPON SODA CO. LTD (NISSO), a Japanese company of 2-7-2, Marunouchi, Chiyoda-ku, Tokyo 100-7010, Japan

PREAMBLE TO THE DESCRIPTION
The following specification particularly describes the invention and the manner in which it is to be performed:

FIELD OF THE INVENTION
[001] The present invention relates to a fungicidal composition, more particularly to a synergistic fungicidal composition.

BACKGROUND OF THE INVENTION
[002] One of the most effective strategies for reducing severe poverty and increasing shared prosperity is agricultural development. As a result of the necessity to produce high quality and quantity of grains per unit area to feed the enormous global population, agricultural setup is changing extremely quickly in the modern agricultural system. Compared to other sectors, the agriculture sector's growth is two to four times more successful at increasing the incomes of the poorest people. Economic expansion also depends heavily on agriculture. But due to different issues like pests, fungi, the environment, and other external causes, agriculture-driven growth, poverty reduction, and food security are at risk. Fungi can cause serious damage in agriculture, resulting in critical losses of yield, quality, and profit. The agriculture industry uses fungicides for preventing/controlling the menace of fungi and thereby achieving higher yields by reducing/eliminating yield losses.
[003] But there is no control over the number of fungicides used by the cultivators for increased yield. Fungicides have unintended impacts on plants, animals, and the environment when used excessively and irrationally. The fungicides are to blame for residual issues, the emergence of pathogen resistance, and various health risks to people and other living things. Fungicides must be used carefully together with Integrated Pest Management (IPM) techniques in order to prevent these off-target effects of fungicides. High field performance, the environment, and the safety of mammals will always be crucial concerns in the creation of novel fungicide compounds. High-throughput screening and combinatorial chemistry are crucial methods for creating fungicides with novel mechanisms of action that are both safe and efficacious.
[004] The crops have become more susceptible to these illnesses because of overuse of this strategy, though, as the new crops have lost their natural resilience and the fungi developed tolerance to the fungicides. The agricultural output has significantly decreased because of this resistance. Through natural selection, fungi develop resistance. Globally, this kind of resistance is growing, which always lowers crop productivity yield. Food intended for human consumption has been discovered to contain fungicide residues, mostly from post-harvest treatments. Some fungicides, including the now discontinued vinclozolin, are harmful to human health.
[005] Thus, there is a need to increase the efficacy of the fungicides by broadening their spectrum, for effectively controlling the growth of fungi efficiently. A single active ingredient composition rarely meets such a wide range of requirements.
[006] Therefore, a composition containing two or more active ingredients is required. In addition, such compositions containing two or more active ingredients have several additional advantages like reduced frequency of spraying fungicides on crops. This problem of multiple spraying of crops is often overlooked, resulting in inconsistent application of two different active ingredients, resulting in different results and yields. The stable formulation will ensure enhanced absorption and help in reducing stress by enhancing the plant resistance and achieve high crop yield and quality standards.
[007] The present invention meets this need for combination products involving different fungicides and having synergistic effect which can be widely applicable in the field of agriculture for controlling wide spectra of fungi and which also exhibits good physical and chemical stability.

SUMMARY OF THE INVENTION
[008] In one aspect, the present invention is directed to a synergistic fungicidal composition comprising a tetrazolyl oxime, a cyanoacetamide oxime, a plant promoting agent or stabilizer, and at least one agrochemically acceptable excipient.
[009] In an embodiment, the tetrazolyl oxime is picarbutrazox.
[010] In another embodiment, the cyanoacetamide oxime is cymoxanil.
[011] In yet another embodiment, the plant promoting agent or stabilizer is selected from the group consisting of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, boron, chlorine, iron, manganese, zinc, copper, molybdenum, nickel, and salts thereof.
[012] In still another embodiment, the at least one agrochemically acceptable excipient is selected from carrier, defoamer, wetting agent, disintegrating agent, anti-caking agent, dispersing agent, colorant, polymeric film forming agent emulsifier, surfactant, oil, preservative, thickener, viscosity modifying agent, rheology modifying agent, anti-freezing agent, buffering agent, diluent and mixtures thereof.
[013] In a further embodiment, the pesticidal composition is formulated in a dosage form selected from the group consisting of suspension concentrate, water dispersible granule, wettable powder, emulsifiable concentrate, emulsion in water, dual emulsion, ZC formulation, capsule suspension, suspoemulsion, oil dispersion, flowable suspension, water dispersible powder for slurry treatment, and powder for dry seed treatment.
[014] In a still further embodiment, the synergistic fungicidal composition comprises 1 wt.% to 50% wt.% of the tetrazolyl oxime; 1 wt.% to 60% wt.% of the cyanoacetamide oxime; 1 wt.% to 20% wt.% of the plant promoting agent or stabilizer, and at least one agrochemically acceptable excipient. The wt.% is based on the total weight of the composition.
[015] In another aspect, the present invention is directed to a method for preparing the above synergistic fungicidal composition comprising mixing the following: tetrazolyl oxime, cyanoacetamide oxime, a plant promoting agent or stabilizer, and at least one agrochemically acceptable excipient.
[016] In an embodiment, the method comprises the following sub-steps:
(A) grinding tetrazolyl oxime, and cyanoacetamide oxime to obtain a grounded mixture,
(B) mixing the grounded mixture with the plant promoting agent or stabilizer, and at least one agrochemically acceptable excipient to obtain a mixture, and
(C) extruding a dough containing the mixture and water to obtain water dispersible granules of the synergistic fungicidal composition.
[017] In another embodiment, the method comprises the following sub-steps:
(A) mixing the plant promoting agent or stabilizer, at least one agrochemically acceptable excipient, and water to obtain a premixture,
(B) mixing the premixture with tetrazolyl oxime followed by cyanoacetamide oxime to obtain a slurry mixture, and
(C) milling the slurry mixture to obtain a particle size distribution Dv50 of less than 5 microns and Dv90 of less than 10 microns.
[018] In a further aspect, the present invention is directed to a method of controlling and eliminating fungal disease from plants. The method comprises applying to the soil, a locus of infection, a plant and/or propagation material thereof susceptible to attack by fungi, an effective amount of the above synergistic fungicidal composition.
[019] In a still further aspect, the present invention is directed to the use of the above synergistic fungicidal composition for controlling fungi.

DETAILED DESCRIPTION OF THE INVENTION
[020] Before the compositions and formulations of the present invention are described, it is to be understood that this invention is not limited to particular compositions and formulations described, since such compositions and formulations may, of course, vary. It is also to be understood that the terminology used herein is not intended to be limiting since the scope of the present invention will be limited only by the appended claims.
[021] The terms “comprising”, “comprises” and “comprised of” as used herein are synonymous with “including”, “includes” or “containing”, “contains”, and are inclusive or open-ended and do not exclude additional, non-recited members, elements or method steps. It will be appreciated that the terms “comprising”, “comprises” and “comprised of” as used herein comprise the terms “consisting of”, “consists” and “consists of”.
[022] Furthermore, the terms “first”, “second”, “third” or “(a)”, “(b)”, “(c)”, “(d)” etc. and the like in the description and in the claims, are used for distinguishing between similar elements and not necessarily for describing a sequential or chronological order. It is to be understood that the terms so used are interchangeable under appropriate circumstances and that the embodiments of the invention described herein are capable of operation in other sequences than described or illustrated herein. In case the terms “first”, “second”, “third” or “(A)”, “(B)” and “(C)” or “(a)”, “(b)”, “(c)”, “(d)”, “i”, “ii” etc. relate to steps of a method or use or assay there is no time or time interval coherence between the steps, that is, the steps may be carried out simultaneously or there may be time intervals of seconds, minutes, hours, days, weeks, months or even years between such steps unless otherwise indicated in the application as set forth herein above or below.
[023] In the following passages, different aspects of the present invention are defined in more detail. Each aspect so defined may be combined with any other aspect or aspects unless clearly indicated to the contrary. In particular, any feature indicated as being preferred or advantageous may be combined with any other feature or features indicated as being preferred or advantageous.
[024] Reference throughout this specification to “one embodiment” or “an embodiment” means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, the appearances of the phrases “in one embodiment” or “in an embodiment” in various places throughout this specification are not necessarily all referring to the same embodiment but may. Furthermore, the particular features, structures or characteristics may be combined in any suitable manner, as would be apparent to a person skilled in the art from this disclosure, in one or more embodiments. Furthermore, while some embodiments described herein include some, but not other features included in other embodiments, combinations of features of different embodiments are meant to be within the scope of the invention and form different embodiments, as would be understood by those in the art. For example, in the appended claims, any of the claimed embodiments can be used in any combination.
[025] Furthermore, the ranges defined throughout the specification include the end values as well, i.e., a range of 1 to 10 implies that both 1 and 10 are included in the range. For the avoidance of doubt, the applicant(s) shall be entitled to any equivalents according to applicable law.
[026] An aspect of the present invention relates to a synergistic fungicidal composition comprising: a tetrazolyl oxime; a cyanoacetamide oxime; a plant promoting agent or stabilizer; and at least one agrochemically acceptable excipient.
[027] In an embodiment, the composition includes the aforementioned ingredients in suitable amounts which render the composition effective as a fungicide. For instance, the composition comprises 1 wt.% to 50% wt.% of the tetrazolyl oxime; 1 wt.% to 60% wt.% of the cyanoacetamide oxime; and 1 wt.% to 20% wt.% of the plant promoting agent or stabilizer. The least one agrochemically acceptable excipient is present in amounts to make the total weight of the composition as 100 wt.%. The wt.% of the ingredients in the composition should be considered to be based on the total weight of the composition, unless provided otherwise.
[028] The tetrazolyl oxime fungicide of the present invention comprises broad spectrum, systemic fungicides which have activity against Pythium, Phytophthora, Bremia, Peronospora, Pseudoperonspora Fungi and are useful in control of many fungal disease in a wide range of field crops and fruits. In an embodiment, the tetrazolyl oxime is picarbutrazox.
[029] Suitable amounts of the tetrazolyl oxime fungicide may be added in the composition. In an embodiment, the tetrazolyl oxime fungicide is present in an amount ranging between 1 wt.% to 45% wt.%, or 5 wt.% to 45% wt.%, or 5 wt.% to 40% wt.%. In another embodiment, the tetrazolyl oxime fungicide is present in an amount ranging between 10 wt.% to 40% wt.%, or 15 wt.% to 40% wt.%, or 20 wt.% to 40% wt.%. In yet another embodiment, the tetrazolyl oxime fungicide is present in an amount ranging between 1 wt.% to 30 wt.%, or 5 wt.% to 15 wt.%.
[030] In another embodiment, the cyanoacetamide oxime fungicide used in the present invention comprise of broad spectrum, systemic fungicides which have activity against Downy Mildew (Pseudoperonospora cubensis). In an embodiment, the cyanoacetamide oxime fungicide is cymoxanil.
[031] Suitable amounts of cyanoacetamide oxime may be added in the composition. In an embodiment, the cyanoacetamide oxime is present in an amount ranging between 1 wt.% to 55% wt.%, or 5 wt.% to 55% wt.%, or 5 wt.% to 50% wt.%. In another embodiment, the cyanoacetamide oxime is present in an amount ranging between 10 wt.% to 50% wt.%, or 15 wt.% to 50% wt.%, or 20 wt.% to 50% wt.%. In yet another embodiment, the cyanoacetamide oxime is present in an amount ranging between 1 wt.% to 25 wt.%, or 1 wt.% to 30 wt.%.
[032] In an embodiment, the plant growth promoting agent acts as a stabilizer. Suitable plant growth promoting agent or stabilizer can be selected from the group consisting of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, boron, chlorine, iron, manganese, zinc, copper, molybdenum, nickel, and salts thereof.
[033] In another embodiment, the plant growth promoting agent or stabilizer is selected from the group consisting of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, boron, chlorine, iron, manganese, zinc, and salts thereof.
[034] In yet another embodiment, plant growth promoting agent or stabilizer can be selected from the group consisting of nitrogen, phosphorus, potassium, sulfur, chlorine, iron, manganese, zinc, and salts thereof.
[035] Suitable examples of salts in the context of plant growth promoting agent or stabilizer include sodium chloride, sodium hydrogen sulphate, magnesium nitrate, sodium carbonate; potassium acetate, potash alum, dolomite; complex salts. gypsum, washing soda, calcium chloride, magnesium chloride, zinc chloride potassium carbonate, calcium oxide, silica gel, copper sulphate, magnesium sulphate, calcium hydroxide chloride, ammonium chloride, sodium hydrogen carbonate, bleaching powder, cuprous chloride, sodium sulphate, ammonium sulphate, ferrous sulphate, zinc sulphate, calcium oxychloride, calcium sulphate, sodium chloride, Mohr’s salt, and ferric alum. In a preferred embodiment, the plant growth promoting agent or stabilizer is zinc sulphate or salts thereof.
[036] In another embodiment, the synergistic fungicidal composition comprises: 1 wt.% to 50% wt.% of tetrazolyl oxime; 1 wt.% to 60%wt.% of cyanoacetamide oxime; 1 wt.% to 20wt.% of the plant promoting agent or stabilizer; and at least one agrochemically acceptable excipient, wherein the wt.% is based on the total weight of the composition.
[037] In another embodiment, the synergistic fungicidal composition comprises: 1 wt.% to 50 wt.% of tetrazolyl oxime; 1 wt.% to 60 wt.% of cyanoacetamide oxime; 1 wt.% to 20 wt.% of the plant promoting agent or stabilizer; and at least one agrochemically acceptable excipient selected from the group comprising of wetting agent, dispersing agent, anti-freezing agent, antifoam, preservative, rheology modifier, diluent, buffering agent, and surfactant or emulsifier; The wt.% is based on the total weight of the composition.
[038] In another embodiment, the synergistic fungicidal composition comprises: 1 wt.% to 50 wt.% of tetrazolyl oxime; 1 wt.% to 60 wt.% of cyanoacetamide oxime; 1 wt.% to 10 wt.% of the plant promoting agent or stabilizer; and at least one agrochemically acceptable excipient, wherein the wt.% is based on the total weight of the composition.
[039] In another embodiment, the synergistic fungicidal composition comprises: 1 wt.% to 50 wt.% of picarbutrazox; 1 wt.% to 60 wt.% of cymoxanil; 1 wt.% to 20 wt.% of the plant promoting agent or stabilizer; and at least one agrochemically acceptable excipient, wherein the wt.% is based on the total weight of the composition.
[040] In another embodiment, the synergistic fungicidal composition comprises: 1 wt.% to 50 wt.% of picarbutrazox; 1 wt.% to 60 wt.% of cymoxanil; 1 wt.% to 20 wt.% of the plant promoting agent or stabilizer selected from nitrogen, phosphorus, potassium, sulfur, chlorine, iron, manganese, zinc, and salts thereof; and at least one agrochemically acceptable excipient, wherein the wt.% is based on the total weight of the composition.
[041] In another embodiment, the synergistic fungicidal composition comprises: 1 wt.% to 50 wt.% of picarbutrazox; 1 wt.% to 60 wt.% of cymoxanil; 1 wt.% to 20 wt.% of the plant promoting agent or stabilizer which is selected from zinc, and salts thereof; and at least one agrochemically acceptable excipient, wherein the wt.% is based on the total weight of the composition.
[042] The composition also includes at least one agrochemically acceptable excipient. In an embodiment, the composition includes more than one agrochemically acceptable excipient. A person skilled in the art is aware of the suitable agrochemically acceptable excipients that may be included in a typical fungicidal composition. In an embodiment, the agrochemically acceptable excipient can be selected from the group consisting of carrier, defoamer, wetting agent, disintegrating agent, anti-caking agent, dispersing agent, colorant, polymeric film forming agent, emulsifier sticking agent, surfactant, surfactant, oil, preservative, thickener, viscosity modifying agent, rheology modifying agent, anti-freezing agent, buffering agent, diluent and mixtures thereof. The agrochemically acceptable excipients in a pre-determined ratio aid in improving the physical stability and also prevent the degradation of the composition, thereby leading to long term stability and spectrum activity.
[043] In an embodiment, the agrochemically acceptable excipients is present in an amount ranging between 1 wt.% to 80 wt.%. Excipients in the context of fungicidal composition are well known to the present invention. However, the preceding paragraphs provide a list of suitable excipients which may be used in the present composition.
[044] In one embodiment, the antifoam/defoamer is selected from silicon emulsions, poly dimethyl siloxane powder or liquid, and silicon base antifoam.
[045] In one embodiment, the wetting agent is selected from polyalkylene oxide block copolymer, salts of di-octyl sulfosuccinate, alcohol alkoxylate diester, sodium n-methyl oleoyl taurate, sodium cocoyl sarcosinate, sodium lauryl sulphate, sodium dioctyl sulfosuccinate, alkyl polyglucoside, naphthalenesulfonic acids, branched and linear butyl derivatives sodium salts, and sodium lauryl sulphate.
[046] In one embodiment, the disintegrating agent is selected from sodium chloride, sodium sulphate, ammonium sulphate, sodium carbonate, sodium bicarbonate, sodium tripolyphosphate (STPP), crosslinked carboxyl methyl cellulose, crosslinked PVA, crosslinked starch, crosslinked alginic acid and calcium silicate.
[047] In one embodiment, the binder or film forming agent is selected from polyvinyl alcohol, polyvinylpyrrolidone, starch, and dextrin.
[048] In one embodiment, the buffering agent is selected from sodium acetate, potassium acetate; ammonium carbonate, calcium carbonate, sodium carbonate, acidic buffers such as mono carboxylic, dicarboxylic acid, succinic acid, citric acid, fumaric acid, glycine, phosphate-based buffers, potassium phosphate, glacial acetic acid, boric acid, hydroxy carboxylic functionality base, and sulphonic base.
[049] In one embodiment, the colorant is selected from organic pigment, inorganic pigment and metallic pigment, acid dues, azoic dyes, phthalocyanine dye, cationic dyes, fluorescent, anthraquinone, cellulose, and aniline dyes.
[050] In one embodiment, the emulsifiers is selected from polyoxyethylene alkylamine, polyoxyethylene linear alkyl ether, ethoxylated castor oil, epoxidized soyabean oil, polyoxyethyene alkylamine quaternary, polyoxyethyene tallow ethylmonium ethosulfate, glycerol monostearate, glycerol monooleate, ethoxylated monostearate / distearate / tri stearate ethoxylated monooleate / trioleate, polyoxyethylene branched tridecyl phosphate, polyoxyethylene branched tridecyl phosphate neutralised, polyoxyethylene alkyl ether phosphate, ethoxylated propoxylated sorbitan mono esters, and tallow amine ethoxylate.
[051] In one embodiment, the dispersing agent is selected from anionic graft polymer, kraft lignin polymer, napthalene sulphonate formaldehyde condensate, amine alkyl benzenesulfonate, sodium lignosulphonate, blend of naphthalene sulphonate sodium salts, blend of lignin sulphonates sodium salts, ethoxylated lignin sulphonates, linear alkyl benzene sulphonate, tri stearyl phenol ethoxylates, acrylic copolymer solution, modified styrene acrylic polymer, ammonium distyrylphenyl ether sulphate, and salts of polystyrene sulphonic acids.
[052] In one embodiment, the antifreeze agent is selected from propylene glycol, 1-methoxyl-2-propoanol, DL-propanedioil- (1,2), butyl cellosolve, and ethylene glycol.
[053] In one embodiment, the preservatives are selected from 1-2-benziisothiazolin-3-one, 5-Chloro-2-methyl-4-isothiazolin-3-one, and 2-methyl-4-isothiazolin-3-one.
[054] In one embodiment, the rheology modifier is selected from polyester block co-polymer, poly saccharides, polyamide, clay, xanthum gum, and silicates.
[055] In one embodiment, the binder / film-forming agent is selected from hydrophilic polymers, hydrobhobic polymers, poly(vinylpyrrolidone), and vinypyrrolidone-vinylacetate.
[056] In one embodiment, the solvents can be selected from aromatic hydrocarbon solvent such as SOLVESSO®, paraffinic hydrocarbon solvent such as EXXSOL®, cyclic hydrocarbon solvent such as cyclohexanone, N-methylpyyrolidone, mixed xylene, cyclohexane, Dimethyl sulphoxide, Diemthyl formamide, toluene, ethyl methyl ketone, and Tetrahydrofuran. Other solvent / co solvent can be selected from N-butyl Pyrrolidone, Dimethylamide of C8/C10 fatty acid, Dimethylamide of C10 fatty acid, Dimethyl Amide, Dipropylene Glycol, Caprylic/Capric Triglyceride, Canola Oil Methyl Ester, Methyloleate/Linoleate Methyl Ester, N,N-Dimethyl 9-Dodecenamide, Methyl Caprylate/Caprate, N,N-Dimethyloctanamide (N,N-Dimethylcaprylamide), N,N-Dimethyl-Decanamide [(N,N-Dimethylcapramide)], alkanes, and alkanols.
[057] In one embodiment, the co-solvent can be selected from bicyclic tetraether, alkanes, alkenes, dibasic ester solvents, alkylene carbonates including esters, hydrocarbons amides, aldehyde, ketones, ether, esters, carbonate based, aromatic ester solvent, benzyl acetate, dimethyl propylene mono methyl ether, formylmorpholine, 2-ethylhexyl ester of natural l-lactic acid.( ethtyl hexyl lactate), alcohol, ethyl acetate, polyethylene glycol, chloroform, and chlorinated hydrocarbons.
[058] In one embodiment, the filler/carrier is selected from starch and their derivatives, sugars and sugar alcohols, silicates, calcium phosphates, calcium sulfate, dextrates, kaolin clay, bentonite clay, attapulgite, diatomaceous earth, magnesium carbonate, polymethacrylates, talc, and salts.
[059] In the present context, water is used as a diluent to dilute the active ingredient to a desired concentration. Water used is purified water and includes deionized water and/or distilled water.
[060] In an embodiment, the composition is formulated in a dosage form selected from the group consisting of suspension concentrate, emulsifiable concentrate, emulsion in water, dual emulsion, water dispersible granule, wettable powder, ZC formulation, capsule suspension, suspoemulsion, oil dispersion, flowable suspension, water dispersible powder for slurry treatment, and powder for dry seed treatment. The person skilled in the art is well aware of suitable techniques for making these dosage forms.
[061] Another aspect of the present invention relates to a method for preparing the synergistic fungicidal composition, as above. Accordingly, the embodiments pertaining to the synergistic fungicidal composition are applicable here as well.
[062] In an embodiment, the method comprises at least the step of mixing the following ingredients: tetrazolyl oxime, cyanoacetamide oxime, a plant promoting agent or stabilizer, and at least one agrochemically acceptable excipient. In another embodiment, the aforementioned ingredients may be used in suitable and/or predetermined amounts which is capable of imparting enhanced fungicidal and synergistic effect to the overall composition.
[063] In another embodiment, the method comprises the following sub-steps:
(A) grinding tetrazolyl oxime, and cyanoacetamide oxime to obtain a grounded mixture,
(B) mixing the grounded mixture with the plant promoting agent or stabilizer, and at least one agrochemically acceptable excipient to obtain a mixture, and
(C) extruding a dough containing the mixture and water to obtain water dispersible granules (WG) of the synergistic fungicidal composition.
[064] Herein, "grinding" refers to a mechanical process involving the reduction of particle size of the ingredients of the composition. This reduction in particle size may enhance the dispersibility, solubility, or efficacy of the fungicidal agents within the composition. Grinding in this context can encompass various techniques such as milling, pulverizing, or comminution, typically performed using equipment such as mills, crushers, or grinders. The person skilled in the art is well aware of suitable grinding techniques that may be employed for this purpose.
[065] Further, “extruding the dough” refers to a technique wherein the mixture is forced through a die or a shaping orifice under pressure to produce a desired shape or form. This process is akin to the extrusion of materials in the manufacturing industry, where the dough-like mixture is forced through a specifically designed nozzle or aperture to create a continuous shape, such as rods, pellets, granules, or other forms suitable for application as a fungicidal composition. Extrusion is utilized to achieve uniformity in the composition's physical characteristics and distribution of active ingredients, thereby enhancing its efficacy and usability. The person skilled in the art is well aware of suitable extruding techniques that may be employed for this purpose.
[066] In a further embodiment, the method comprises the following sub-steps:
(A) mixing the plant promoting agent or stabilizer, at least one agrochemically acceptable excipient, and water to obtain a premixture,
(B) mixing the premixture with tetrazolyl oxime followed by cyanoacetamide oxime to obtain a slurry mixture, and
(C) milling the slurry mixture to obtain a particle size distribution Dv50 of less than 5 microns and Dv90 of less than 10 microns.
[067] The aforementioned method steps result in suspension concentrate (SC) dosage form of the composition.
[068] Still another aspect of the present invention relates to a method of controlling and eliminating fungal disease from plants.
[069] In an embodiment, the method comprises applying to the soil, a locus of infection, a plant and/or propagation material thereof susceptible to attack by fungi, an effective amount of the synergistic fungicidal composition, as described above. Accordingly, the embodiments pertaining to the composition are applicable here as well.
[070] Yet another aspect of the present invention relates to the use of synergistic fungicidal composition for controlling fungi. Accordingly, the embodiments pertaining to the composition are applicable here as well.
[071] Advantageously, the present invention provides a composition containing two or more active ingredients which has several benefits, such as but not limited to, effective control of fungal pathogens, broad-spectrum activity against various fungal species, specificity and selectivity towards target fungi while minimizing harm to non-target organisms, residual activity for prolonged protection, compatibility with other agricultural inputs or industrial processes, stability and longevity under diverse environmental conditions, ease of application, and cost-effectiveness by reducing crop losses or increasing yields. The present composition further provides a reliable, safe, and efficient composition for controlling fungal diseases in agricultural settings, contributing to enhanced productivity, sustainability, and profitability.
EXAMPLES
[072] The present invention is more particularly described in the following examples that are intended as illustration only, since numerous modifications and variations within the scope of the present invention will be apparent to those skilled in the art. Unless otherwise noted, all parts, percentages and ratios reported in the following examples are on a weight basis, and all ingredients used in the examples were obtained or are available from the chemical suppliers.
[073] The following examples illustrate the basic methodology and versatility of the present invention.
[074] Example 1: WG formulation Picarbutrazox 15% + Cymoxanil 50% WG
[075] Suitable amount of Picarbutrazox and Cymoxanil was ground in an air jet mill to get desired particle size. Subsequently, the ground mixture containing Picarbutrazox (15.50 g), and Cymoxanil (51.15 g) was mixed with Potassium Polycarboxylate (5.0 g), Naphthalene sulfonate formaldehyde condensate (5.0 g), Dispersant MF 5 (5.0 g), Sodium Lauryl Sulfate (1 g), Citric Acid Anhydrous (1.8 g), Sodium Polytriphosphate (4.0 g), Zinc Sulphate Monohydrate (1.0 g), Lactose Monohydrate (9.05 g), Dimethyl siloxane emulsion (1.50 g) into the ribbon blender and blended for about 30 min to obtain a mixture. Then mixture was transferred to a dough maker and DM Water was added (14. g) followed by blending till proper extrudable dough was formed (approx. time 15 min.) Thereafter, the dough was charged in feed chamber of basket extruder to get extruded granules.
[076] Granules were collected in a plastic tray at the base of basket extruder. The received granules were transferred in a Fluid Bed Dryer and dried at 50 (±5) °C for approx. 10 min till the moisture content in the dried granules is 2% or less. These granules were sieved through and analysed for several parameters.
[077] Example 2: SC Formulation Picarbutrazox 15% + Cymoxanil 20% w/v SC
[078] Suitable amounts of DM Water, Zinc Sulphate Monohydrate (1.0 g), EO-PO Block copolymer (2.0 g), Polymethyl methacrylate-polyethylene glycol, graft copolymer (3.0 g), DL-Propanediol-(1,2) (5.0 g), Polydimethylsiloxane emulsion (1.0 g), 1,2 -benzisothiazole-3-one (0.10 g) were charged in a premixing vessel and stirred the mixture at 600 (± 100) rpm for 30 min to obtain the premixture. Thereafter, Picarbutrazox (15 g) was added gradually with continuous stirring. Subsequently, Cymoxanil (20. g) was added gradually with continuous stirring at 600 (± 100) rpm for 30 min to obtain a slurry mixture.
[079] The slurry mixture was then charged in a feed chamber of wet mill for particle size reduction to obtain desired particle size: Dv50< 5 µm and Dv90 <10µm. The ground slurry was transferred in a post mixing vessel and stirred at 700 (± 100) rpm for 30 min. Subsequently, polysaccharide gum was added and stirred at 700 (± 100) rpm for 2 h. The SC formulation obtained was analysed for several parameters.
[080] Several SC forms of the composition were prepared, and various parameters were studied. Moreover, comparative compositions were also studied, and results summarized in the Table below.
Table 1: SC formulations and parameter evaluation
Source: Comparative 3 Inventive 1 Comparative 1 Inventive 2 Comparative 2
Name of Products Picarbutrazox 200 + Cymoxanil 200 SC Picarbutrazox 150 + Cymoxanil 250 SC Picarbutrazox 150 + Cymoxanil 250 SC Picarbutrazox 150 + Cymoxanil 200 SC Picarbutrazox 150 + Cymoxanil 200 SC

Ratio 50:50 62.50 : 37.50 62.50 : 37.50 57.14 : 42.86 57.14 : 42.86
Cymoxanil tech. 20 25.00 25.00 20.00 20.00
Picarbutrazox tech. 20 15.00 15.00 15.00 15.00
Calcium dodecyl benzene sulfonate 3.00 0.00 0.00 0.00 0.00
Glyceryl fatty acid ester polyoxyethylene ether 4.00 0.00 0.00 0.00 0.00
Ethylene glycol 2.50 0.00 0.00 0.00 0.00
Magnesium aluminum silicate 2.00 0.00 0.00 0.00 0.00
Isothiazolinone 0.5 0.10 0.10 0.10 0.10
Zinc Sulphate Monohydrate (ZnSO4.H2O) 0.00 1.00 0.00 1.00 0.00
EO-PO Block copolymer 0.00 2.00 2.00 2.00 2.00
Polymethyl methacrylate-polyethylene glycol graft copolymer; 0.00 3.00 3.00 3.00 3.00
DL-Propanediol-(1,2) 0.00 5.00 5.00 5.00 5.00
Polydimethylsiloxane emulsion 0.00 1.00 1.00 1.00 1.00
Polysaccharide 0.00 0.20 0.20 0.20 0.20
DM Water Q.S Q.S Q.S Q.S Q.S
Total 100 100.00 100.00 100.00 100.00
Analytical output: RT HT RT HT RT HT RT HT RT HT
Appearance Beige Brown Viscous Liquid Beige Brown Viscous Liquid Beige Brown Viscous Liquid Beige Brown Viscous Liquid Beige Brown Viscous Liquid Beige Brown Viscous Liquid Beige Brown Viscous Liquid Beige Brown Viscous Liquid Beige Brown Viscous Liquid Beige Brown Viscous Liquid
Cymoxanil AI 20.22 17.00 25.07 24.65 24.89 24.61 20.90 20.62 20.11 20.014
Picarbutrazox AI 20.21 13.01 15.2 15.05 15.01 14.98 15.15 15.12 15.14 15.11
pH 1% D/W 6.00 5.97 5.79 6.17 5.75 6.01 5.85 6.21 5.87 5.98
Suspensibility (Gravimetric) 82.00 68.00 85.38 84.22 90.00 79.00 99.67 95.67 90.11 80.33
Persistent Foam Max. ml 6.00 8.00 10 8 10 8 10 10 12 16
Wet Sieve Test (75 Mc µ) Reten. 0.01 0.5 Nil Nil Nil Nil 0.08 0.1 0.05 0.20
Viscosity cp, Spd 63, 60 RPM 100 250 300 374 400 450 341 400 300 420
Particle Size D90 7.28 13 6.22 7.03 8.9 10.00 8.74 10.29 9.00 11.02

[081] As noted in Table 1, formulations devoid of zinc sulphate monohydrate were found to be having inferior properties. For instance, the comparative formulation 3 (having equal dosage of both the active ingredients) resulted in degradation of the active ingredients as well as suspensibility was an issue. The comparative example 1 (having higher dosage of cymoxanil) resulted in stable active ingredient but suspensibility was degraded. Further, the comparative example 2 (having reduced dosage of cymoxanil), having dosage similar to inventive example 2, resulted in degradation of suspensibility. On the contrary, the inventive formulations containing zinc sulphate monohydrate were found to have better suspensibility without any degradation of the active ingredient.
[082] Bioefficacy and phytotoxicity data
[083] The present invention composition was evaluated for bioefficacy and phytotoxicity for potato late blight and downy mildew in grapes. The findings of the evaluation are summarized below.
[084] Potato Late Blight
[085] Table 2: Bio-efficacy Treatment details
S.No. Product Dose/ha
(g a.i.) Formulation
(g or ml)
T1 Picarbutrazox 15% + Cymoxanil 20%SC (Example 1) 75+100 500
T2 Picarbutrazox 10% SC 93.75 937.5
T3 Cymoxanil 50% WP 1200 2400
T4 Picarbutrazox 10% SC + Cymoxanil 50% WP (Tank Mixture) 93.75 + 1200 937.5+2400
T5 Untreated Control - -

[086] A ready mix of two-way combinations, Picarbutrazox 15%, Cymoxanil 20% SC, were tested at specified concentrations, along with one possible tank mix combinations: Picarbutrazox 10% SC + Cymoxanil 50% WP. Additionally, solo individual fungicides, namely Picarbutrazox 10% SC, Cymoxanil 50% WP, and an untreated control, were tested against late blight in potato. The fungicides were applied as foliar spray using a Knapsack Sprayer fitted with a hollow cone nozzle. The spraying schedule was initiated at the onset of disease in the field and applications given at 10-day intervals. The appearance of the visible symptoms of the disease incidence before the 1st spray and subsequent observations were recorded 10 days after subsequent spray and 7th and 12th day after 3rd spray by adopting 0-9 scale.
[087] Table 3: Disease Ratings: Late blight of Potato
Scale (Grade) % Infection
0 Nil
1 1-5% infection,
3 6-10% infection,
5 11-25 infection,
7 26-50% infection
9 more than 50 % infection.

[088] Percent disease index (PDI) was calculated using the following formula:
Sum of all numerical ratings
PDI = --------------------------------------------------------------------- X 100
Total plants observed X Maximum rating scale

[089] The PDI values were transformed by angular transformation and analyzed statistically. The disease control DC (%) was calculated by the following formula:
Disease % in control - Disease % in treatment
DC (%) = ------------------------------------------------------------- X 100
Disease % in control

[090] Table 4: Bio-efficacy of different Fungicides treatments against Late blight of Potato

[091] In Table 4, figures in parenthesis are arcsine transformed values. PDI is Percent Disease Incidence, PDC is Percent Disease Control, DAA is Days After Application, NS is non-significant.
[092] As shown in Table 4, at the time of initiation of trial the uniform disease incidence was observed which was statistically on par with each other indicating uniformity of trial plot across all the treatments. 10 days after 1st application, the highest Late blight incidence was recorded in control (15.27 %). All the fungicide treatments significantly reduced the disease severity than untreated control, but the significant lowest late blight disease intensity was observed in T1 with 3.17 % disease incidence which is statistically on par with T4 (Picarbutrazox 10% SC + Cymoxanil 50%) with 3.28 % incidence.
[093] At 10 days after second application, the highest disease was recorded in control (34.98 %). The significant lowest late blight disease incidence was observed in T1 with 7.85% disease intensity, which was statistically on par with the tank mix treatment T4 with 7.98% disease intensity.
[094] At 12 days after 3rd application also similar trend was observed as of after second application. Whereas the significant lowest late blight disease incidence was observed in T1 (12.62 %) which was statistically superior to remaining treatments.
[095] The highest percent disease control (PDC) was recorded in treatment T1 with 79.44%, followed by T4 with 76.12% PDC. The lowest disease control was recorded in treatment T3 (59.56%), followed by T2 (61.64 %).
[096] Table 5: Phytotoxicity treatment details
S.No. Product Dose/ha
(g a.i.) Formulation
(g or ml)
T1 Picarbutrazox 15% + Cymoxanil 20%SC (RM) 75+100 500
T2 Picarbutrazox 15% + Cymoxanil 20%SC (RM) 150+200 1000
T3 Picarbutrazox 10% SC + Cymoxanil 50% WP (Tank Mixture) 93.75 + 1200 937.5+2400
T4 Picarbutrazox 10% SC + Cymoxanil 50% WP (Tank Mixture) 187.5 + 2400 1875+4800
T5 Untreated Control - -

[097] Phytotoxicity: Observations were taken on damage caused to plants, if any, by the application of different treatments taking into the account phytotoxic symptoms viz. leaf injury on tips and leaf surface, wilting, vein clearing, necrosis, epinasty and hyponasty on ten plants per plot. The observations were recorded before spray and 1, 3, 5, 7, 10 & 15th day after applications. For Phytotoxicity study on leaf injury on tips and leaf surface the Scale (0-10) used is given below.
[098] Table 6: Phytotoxicity Rating Scale (PRS)
Crop response/ Crop injury Rating
0-00 0
1-10% 1
11-20% 2
21-30% 3
31-40% 4
41-50% 5
51-60% 6
61-70% 7
71-80% 8
81-90% 9
91-100% 10

[099] Table 7: Phyto-toxicity effect of different Fungicide treatments on Potato


[0100] In Table 7, phototoxic symptoms- Leaf injury on tips and Leaf surface, Wilting, Vein Clearing, Necrosis, Epinasty and Hyponasty.
[0101] As shown in Table 7, the two-way ready-mix combination was sprayed at doses of X (500 ml/ha) and 2X (1000 ml/ha) to check the phytotoxic effects like leaf injury on tips/surface, vein clearing, wilting, necrosis, hyponasty and epinasty on the Potato crop. The observations on these phytotoxicity parameters were observed on before spray and at 1, 3, 5, 7, 10 and 15 days after application. But there was no phytotoxicity observed on Potato crop after spraying. There was even no adverse effect noticed on Potato crop in the field applied with fungicides combinations at highest dose of @1000 ml/ha.
[0102] Yield: Yield data were recorded on a per-plot basis, and treatment-wise yields were calculated and converted into yield per hectare (t/ha) at harvest. The data were then subjected to statistical analysis.










[0103] Table 8: Effect of different Fungicides treatments on Yield of Potato
Tr. No Treatment Details Dose
(g a.i./ha) Dose
(ml or g/ha) Potato Yield (tons/ha)
T1 Picarbutrazox 15% + Cymoxanil 20%SC 75+100 500 19.96
T2 Picarbutrazox 10% SC 93.75 937.5 16.16
T3 Cymoxanil 50% WP 1200 2400 16.41
T4 Picarbutrazox 10% SC + Cymoxanil 50% WP (Tank Mixture) 93.75 + 1200 937.5+2400 18.09
T5 Untreated Control - - 14.96
CD (P = 0.05) 1.75

[0104] As shown in Table 8, all the treatments significantly increase the yield than Untreated Control (14.96 t/ha). The highest yield was observed in treatment T1 with 19.96 t/ha which was statistically than remaining treatments. The next best treatment was T4 (18.09 t/ha) followed by T3(16.41 t/ha) and T2 (16.16 t/ha). The Ready-mix fungicide treatment (T1) was significantly superior over solo and tank mix fungicide treatments (T2, T3 & T4).
[0105] As reported in Tables 6-8, two-way ready-mix combination of fungicide (Picarbutrazox 15% + Cymoxanil 20%SC) effectively controlled the late blight of Potato. Further, the efficacy of the tested fungicides was far superior than market standards, including Picarbutrazox 10% SC, Cymoxanil 50% WP, and their potential two-way tank mix combination (Picarbutrazox 10% SC + Cymoxanil 50% WP). The tested fungicides significantly increased the yield of potato crop and did not exhibit any phytotoxic effects on potato crops, even at double the recommended dosage. Overall, the two-way combination test fungicides showed synergistic effect for controlling the diseases. To mitigate resistance issues and minimize crop losses, the tested fungicides can be utilized safely and effectively compared to using solo Picarbutrazox 10% SC, Cymoxanil 50% WP, or their two-way tank mix combinations alone.
[0106] Downey mildew in grapes
[0107] Table 9: Bio-efficacy treatment details
S.No. Product Dose/ha
(g a.i.) Formulation
(g or ml)
T1 Picarbutrazox 15% + Cymoxanil 20%SC 75+ 100 500
T2 Picarbutrazox 10% SC 93.75 937.5
T3 Cymoxanil 50% WP 1200 2400
T4 Picarbutrazox 10% SC + Cymoxanil 50% WP (Tank Mixture) 93.75 + 1200 937.5+2400
T5 Untreated Control - -

[0108] Bio-efficacy: A ready mix of two-way combinations, Picarbutrazox 15%, Cymoxanil 20% SC, was tested at specified concentrations, along with one possible tank mix combinations: Picarbutrazox 10% SC + Cymoxanil 50% WP. Additionally, solo individual fungicides, namely Picarbutrazox 10% SC, Cymoxanil 50% WP, and an untreated control, were tested against downy mildew in grapes. The fungicides were applied as foliar spray using a Knapsack Sprayer fitted with a hollow cone nozzle. The spraying schedule was initiated at the onset of disease in the field and applications given at 10-day intervals.
[0109] The appearance of the visible symptoms of the diseases was recorded on 10 leaves and 2 bunches on randomly selected canes. Ten such cane per vine and 3 vines per plot at before the 1st spray and subsequent observations were recorded 10 days after subsequent spray and 10th and 12th day after 3rd spray by adopting 0-9 scale.
[0110] Table 10: Disease Ratings: Downy mildew of Grapes
Scale (Grade) % Infection
0 Nil
1 1-5% infection,
3 6-10% infection,
5 11-25 infection,
7 26-50% infection
9 more than 50 % infection.

PDI, DC and yield were calculated as defined above.
[0111] Table 11: Bio-efficacy of different Fungicides treatments against Downy mildew of Grapes

[0112] In Table 11, figures in parenthesis are arcsine transformed values. PDI is Percent Disease Incidence, PDC is Percent Disease Control, DAA is Days After Application, NS is non-significant.
[0113] As shown in Table 11, at the time of initiation of trial there was uniform disease in all treatments indicates the uniformity of trial plot across all the treatments. At 10 days after application, the highest Downy mildew was recorded in control (12.69%). All the fungicide treatments significantly reduced the disease severity than untreated control, but the significant lowest Downy mildew disease was observed in T1 with 2.82 % disease incidence which is on par with remaining treatments.
[0114] At 10 days after second application, the highest disease was recorded in control (25.87%). The significant lowest Downy mildew disease was observed in T1 (Picarbutrazox 15% + Cymoxanil 20% SC) (4.46%) which was superior to other treatments.
[0115] 14 days after 3rd application also similar trend observed as of after second application. Whereas the significant lowest Downy mildew disease was observed in T1 (8.18%) which was superior to tank mix treatment T4 (Picarbutrazox 10% SC + Cymoxanil 50%).
[0116] The highest percent disease control (PDC) was recorded in treatment T1 with 80.39%, followed by T4 (77.01%). The lowest disease control was recorded in treatment T2 (57.38%), followed by T3 (59.97%).
[0117] Table 12: Phytotoxicity treatment details
S.No. Product Dose/ha
(g a.i.) Formulation
(g or ml)
T1 Picarbutrazox 15% + Cymoxanil 20%SC 75+ 100 500
T2 Picarbutrazox 15% + Cymoxanil 20%SC 150+ 200 1000
T3 Picarbutrazox 10% SC + Cymoxanil 50% WP (Tank Mixture) 93.75 + 1200 937.5+2400
T4 Picarbutrazox 10% SC + Cymoxanil 50% WP (Tank Mixture) 187.5 + 2400 1875+4800
T5 Untreated Control - -

[0118] Phytotoxicity: Observations were taken on damage caused to plants, if any, by the application of different treatments taking into the account phytotoxic symptoms viz. leaf injury on tips and leaf surface, wilting, vein clearing, necrosis, epinasty and hyponasty on ten plants per plot. The observations were recorded before spray and 1, 3, 5, 7, 10 & 15th day after applications. For Phytotoxicity study on leaf injury on tips and leaf surface the Scale (0-10) used is given below.
PRS and statistical analysis was carried out as above.
[0119] Table 13: Phyto-toxicity effect of different Fungicide treatments on Grape

[0120] In Table 13, phototoxic symptoms- Leaf injury on tips and Leaf surface, Wilting, Vein Clearing, Necrosis, Epinasty and Hyponasty.
[0121] As shown in Table 13, the two-way ready-mix combination was applied at rates of X (500 ml/ha) and 2X (1000 ml/ha) to assess potential phytotoxic effects such as leaf injury on tips/surface, vein clearing, wilting, necrosis, hyponasty, and epinasty on Grape crops. Phytotoxicity parameters were evaluated prior to spraying and at 1, 3, 5-, 7-, 10-, and 15-days post-application. However, no phytotoxicity was observed on the Grape crop. Moreover, there were no adverse effects noted on the Grape crop in the field treated with the fungicide combinations, even at the highest dose of 1000 ml/ha.
[0122] Yield: Yield data were recorded on a per-plot basis, and treatment-wise yields were calculated and converted into yield per hectare (t/ha) at harvest. The data were then subjected to statistical analysis.
[0123] Table 14: Effect of different Fungicides treatments on Yield of Grapes
Tr. No Treatment Details Dose
(g a.i./ha) Dose
(ml or g/ha) Grape
Yield (t/ha)
T1 Picarbutrazox 15% + Cymoxanil 20%SC 75+100 500 37.59
T2 Picarbutrazox 10% SC 93.75 937.5 32.57
T3 Cymoxanil 50% WP 1200 2400 32.24
T4 Picarbutrazox 10% SC + Cymoxanil 50% WP (Tank Mixture) 93.75 + 1200 937.5+2400 35.86
T5 Untreated Control - - 30.16
CD (P = 0.05) 1.23

[0124] As shown in Table 14, all the treatments significantly increase the yield than Untreated Control (24.77q/ha). The highest yield was observed in treatment T1 (37.59 t/ha), which was statistically superior to other treatments. T4 recorded yield of 35.86 t/ha followed by T2 (32.57 t/ha) and T3 (32.24 t/ha). Ready mix fungicide treatment (T1) was significantly superior over tank mix and solo fungicide treatments.
[0125] As reported in Table 12-14, the two-way ready-mix combination of the fungicide (Picarbutrazox 15% + Cymoxanil 20% SC) effectively manages Grape downy mildew. The efficacy of the test fungicides was significantly superior to market standards, including Picarbutrazox 10% SC, Cymoxanil 50% WP, and their two-way possible tank mix combination, Picarbutrazox 10% SC + Cymoxanil 50% WP. Further, grape crop yields were higher with the application of the tested fungicides with no observed phytotoxic effects on the Grape crop with the tested fungicides, even when used at double the recommended dosage, was observed. The two-way combination of the tested fungicides demonstrated a synergistic effect in controlling the disease. Furthermore, to address resistance issues and minimize crop losses, the tested fungicides can be safely and effectively used for disease management compared to using solo Picarbutrazox 10% SC, Cymoxanil 50% WP, and their two-way tank mix combinations individually.
[0126] The foregoing description of the invention has been set merely to illustrate the invention and is not intended to be limiting. While the present invention has been described with respect to certain embodiments, it will be apparent to those skilled in the art that various changes and modification may be made without departing from the scope of the invention as defined in the following claims.
,CLAIMS:WE CLAIM:
1. A synergistic fungicidal composition comprising:
a) a tetrazolyl oxime fungicide;
b) a cyanoacetamide oxime fungicide;
c) a plant promoting agent or stabilizer; and
d) at least one agrochemically acceptable excipient.

2. The fungicidal composition as claimed in claim 1, wherein the tetrazolyl oxime fungicide is picarbutrazox.

3. The fungicidal composition as claimed in claim 1, wherein the Cyanoacetamide oxime fungicide is cymoxanil.

4. The fungicidal composition as claimed in claim 1, wherein the plant promoting agent or stabilizer is selected from nitrogen, phosphorus, potassium, calcium, magnesium, sulphur, boron, chlorine, iron, manganese, zinc, copper, molybdenum, and nickel or salts thereof.

5. The fungicidal composition as claimed in claim 1 comprising at least one agrochemically acceptable excipient selected from carrier, defoamer, wetting agent, disintegrating agent, anti-caking agent, dispersing agent, emulsifier, colorant, polymeric film forming agent, surfactant, oil, preservative, thickener, viscosity modifying agent, rheology modifying agent, buffering agent, and anti-freezing agent and/or mixtures thereof.

6. The fungicidal composition as claimed in claim 1, wherein the fungicidal composition is formulated in a dosage form selected from the group consisting of water dispersible granule, wettable powder, emulsifiable concentrate, emulsion in water, dual emulsion, capsule suspension, suspension concentrate, suspoemulsion, oil suspension, capsule suspension, emulsifiable concentrate water, dispersible powder, ZC formulation, oil-in-water emulsion, water-in-oil emulsion, and tank mix.

7. The synergistic fungicidal composition as claimed in claims 1 to 6, wherein said composition comprises:
a) 1 wt.% to 50% wt.% of tetrazolyl oxime;
b) 1 wt.% to 60%wt.% of cyanoacetamide oxime;
c) 1 wt.% to 20wt.% of the plant promoting agent or stabilizer; and
d) at least one agrochemically acceptable excipient,
wherein the wt.% is based on the total weight of the composition.

8. A method for preparing the synergistic fungicidal composition as claimed in claims 1 to 7 comprising mixing the following: tetrazolyl oxime, cyanoacetamide oxime, a plant promoting agent or stabilizer, and at least one agrochemically acceptable excipient.

9. The method as claimed in claim 8, wherein the method comprises the following sub-steps:
(A) grinding tetrazolyl oxime, and cyanoacetamide oxime to obtain a grounded mixture,
(B) mixing the grounded mixture with the plant promoting agent or stabilizer, and at least one agrochemically acceptable excipient to obtain a mixture, and
(C) extruding a dough containing the mixture and water to obtain water dispersible granules of the synergistic fungicidal composition.

10. The method as claimed in claim 8, wherein the method comprises the following sub-steps:
(A) mixing the plant promoting agent or stabilizer, at least one agrochemically acceptable excipient, and water to obtain a premixture,
(B) mixing the premixture with tetrazolyl oxime followed by cyanoacetamide oxime to obtain a slurry mixture, and
(C) milling the slurry mixture to obtain a particle size distribution Dv50 of less than 5 microns and Dv90 of less than 10 microns.

11. A method of controlling and eliminating fungal disease from plants, said method comprising applying to the soil, a locus of infection, a plant and/or propagation material thereof susceptible to attack by fungi, an effective amount of the synergistic fungicidal composition as claimed in claims 1 to 7.

12. Use of the synergistic fungicidal composition claimed in claims 1 to 7 for controlling fungi.

Dated this 26th day of March 2023

Indofil Industries Limited
and
NIPPON SODA CO. LTD (NISSO)
By their Agent & Attorney

(Nisha Austine)
of Khaitan & Co
Reg No IN/PA-1390