DESC:FIELD OF THE INVENTION:

The present invention relates to fungicide composition of bioactive amounts of Dimethomorph and component (A) first fungicide selected from Metalaxyl-M, Cymoxanil, Cyazofamid, Fluopicolide and component (B) second fungicide selected from Captan, Chlorothalonil, Fluazinam, Folpet, Mancozeb, Metiram, Propineb, Ziram, Zoxamide, Boscalid, Azoxystrobin, Pyraclostrobin, Trifloxystrobin. The present invention also relates to process for preparing the said composition comprising bioactive amounts of Dimethomorph and component (A) first fungicide selected from Metalaxyl-M, Cymoxanil, Cyazofamid, Fluopicolide and component (B) second fungicide selected from Captan, Chlorothalonil, Fluazinam, Folpet, Mancozeb, Metiram, Propineb, Ziram, Zoxamide, Boscalid, Azoxystrobin, Pyraclostrobin, Trifloxystrobin with one or more inactive excipients.

BACKGROUND OF THE INVENTION

Dimethomorph was first disclosed in US 4933449 and US4923866.Dimethomorphis chemically known as (EZ)-4-[3-(4-chlorophenyl)-3-(3,4-dimethoxyphenyl)acryloyl]morpholineand having chemical structure as below;

Dimethomorph is a systemic fungicide which protects plants from molds, as well as killing molds on plants and preventing their spread. It is a cinnamic acid derivative and a member of the morpholine chemical family. It is a mixture of two isomers but only the Z isomer has fungicidal activity.

The main concern with the use of fungicide is the development of resistance by the pests for that particular fungicide and at the end one has to apply more concentrated formulation of the fungicide. The high amount of fungicide may results in the toxicity to human beings as well as has bad effect on environment.

Previously people have tried many alternatives and option to overcome this problem and as a result developed poly mixture of pesticide, use of non-toxic ingredients and developing novel formulations which provides effective amount of the fungicide and at the required part only.

However the use of poly mixture containing large number of fungicide poses a problem in many was like preparing formulation of multiple fungicides with different chemical properties and behavior and physical properties. It also creates challenge for formulator in term of compatibility and stability of all the fungicide along with used excipients in the formulation.

CN101953356 relates to a dimethomorph-containing thermal smoke agent for preventing and controlling downy mildew of vinyl house cucumbers. The thermal smoke agent comprises the following active ingredients: dimethomorph and azoxystrobin, dimethomorph and chlorothalonil, dimethomorph and cymoxanil, dimethomorph and metalaxyl, dimethomorph and hymexazol, or dimethomorph and enestroburin, and also comprises the following auxiliaries: an emulsifying agent, a dispersing agent, a solvent, a viscosity regulating agent, a defoaming agent and the like. The active ingredients and the auxiliaries are prepared into an oil-suspending thermal smoke agent, wherein the dimethomorph is mixed with other medicaments in a ratio of 1:3 to 3:1; and the medicaments comprise 20 to 40 percent of active ingredients. The dimethomorph-containing thermal smoke agent is specially used for thermal smoke sprayers and has special effects on the downy mildew of the vinyl house or facility-cultured cucumbers.

CN101653128 relates to relates to a bactericidal composition, the effective ingredients of which are zoxamide and dimethomorph, wherein the mass ratio of the zoxamide to the dimethomorph is 30:1-1:50 and other ingredients are acceptable and allowed auxiliary ingredients in pesticides. The zoxamide and the dimethomorph have obvious synergized action after being compounded in a certain proportion, and are mainly used for preventing and treating oomycete diseases.

CN101617677 discloses relates to sterilization composite which contains tetraconazole and has a synergized effect; the effective activity components of the composite are tetraconazole and dimethomorph; and auxiliary agent and excipient are added into the effective activity components to prepare into aqua resign or microemulsion; wherein the weight ratio of tetraconazole and dimethomorph is 1-20:50-4.

CN101874497 provides to a bactericidal composition containing mandipropamid and dimethomorph and a preparation method and application. The composition is the mixture of the compound (A) mandipropamid and the compound (B) dimethomorph. The composition is characterized in that the weight ratio of the active ingredient mandipropamid to the active ingredient dimethomorph in the composition is 1-50:1-50; the content of the active ingredients in the composition is 1-90%; and the composition can be processed into suspending agents, wettable powder and water-dispersible granules. The bactericidal composition has high bactericidal efficiency and can effectively control the diseases on various crops such as potatoes, tomatoes, cucumbers, grapes, etc. The control effect of the composition is obviously better than that of the single compound (A) or compound (B), thus substantially reducing the application dosage of various active substances.

CN103734164 discloses relates to a pesticide bactericidal suspension liquid comprising the following raw materials in percentage by weight: 15-25% of iprodione, 15-25% of dimethomorph, 4-8% of propanediol butyl ether, 0.05-0.15% of carboxyethyl cellulose, 4-8% of a surfactant and the balance being water. The pesticide bactericidal suspension liquid provided by the invention is high in suspension percentage, good in extensibility and permeability and stable in product performance.

WO198805630 claims the mixture of dimethomorph with several pesticides. Although propamocarb is mentioned as part of a long list of fungicides to be used in association with dimethomorph, no reference is made to the specific association of dimethomorph and propamocarb in its hydrochloride form, nor examples or evidence of the formulation according to the invention are mentioned.

WO2010/128003 A2 relates to a method for increasing the vigor and/or yield of agricultural crops under the conditions of an essentially non-existent pressure from pathogenic agents, comprising a Bacillus subtilis strain, or a derivative thereof, and optionally at least one compound selected from strobilurins, carboxamides, azoles, heterocyclic compounds, carbamates, other active substances, growth regulators, herbicides and insecticides.

WO2015124662 relates to an aqueous co-formulation of metalaxyl, which contains: i. metalaxyl, in particular metalaxyl-M; ii. at least one organic pesticide compound PC1, which has a solubility in water of at most 1 g/l at 20°C and a melting point in the range from 40 to 100°C; and iii. an aqueous phase containing water and at least one surfactant; wherein the at least one organic pesticide compound PC1 is present in the form of particles suspended in the aqueous phase and where metalaxyl is essentially present dissolved in the aqueous phase and where the surfactant comprises at least one salt of an oligomer or polymer having a plurality of arylsulfonyl groups. The invention also relates to a process for preparing such formulations, which comprises the following steps: a) providing a suspension of metalaxyl in an aqueous phase containing water, at least one surfactant, which comprises at least one salt of an oligomer or polymer having a plurality of arylsulfonyl groups, b) treating the suspension until metalaxyl is essentially dissolved, to obtain an aqueous composition of metalaxyl, wherein metalaxyl is present in dissolved form; c) mixing the aqueous composition of metalaxyl obtained in step b) with an aqueous suspension of the further organic pesticide compound PC1.

The present invention does not fall in the scope of the mentioned closest state of the art as it relates to an unique product (three way mixture) in the form of a readily usable composition of Dimethomorph and component (A) first fungicide selected from Metalaxyl-M, Cymoxanil, Cyazofamid, Fluopicolide and component (B) second fungicide selected from Captan, Chlorothalonil, Fluazinam, Folpet, Mancozeb, Metiram, Propineb, Ziram, Zoxamide, Boscalid, Azoxystrobin, Pyraclostrobin, Trifloxystrobin. The references mentioned in the prior art do not disclose any three way mixture nor the synergistic composition according to the present invention, nor the concentration ranges of the active ingredients in the mixture, nor the application rates described herein for an effective fungicidal action.

Inventors of the present invention have surprisingly found that the novel composition of Dimethomorph and component (A) first fungicide selected from Metalaxyl-M, Cymoxanil, Cyazofamid, Fluopicolide and component (B) second fungicide selected from Captan, Chlorothalonil, Fluazinam, Folpet, Mancozeb, Metiram, Propineb, Ziram, Zoxamide, Boscalid, Azoxystrobin, Pyraclostrobin, Trifloxystrobin as described herein in can provide solution to the above mentioned problems.

SUMMARY OF THE INVENTION

It is an object of the present invention to provide, with a view to effective resistance management and effective control of phytopathogenic harmful fungi, at application rates which are as low as possible, compositions which, at a reduced total amount of active compounds applied, have improved activity against the harmful fungi (synergistic mixtures).

We have accordingly found that this objective is achieved by the three way mixtures and compositions, defined herein, comprising Dimethomorph and component (A) first fungicide and component (B) second fungicide as disclosed above.

The main aspect of the present invention is a synergistic fungicide composition comprising Dimethomorph and component (A) first fungicide selected from Metalaxyl-M, Cymoxanil, Cyazofamid, Fluopicolide and component (B) second fungicide selected from Captan, Chlorothalonil, Fluazinam, Folpet, Mancozeb, Metiram, Propineb, Ziram, Zoxamide, Boscalid, Azoxystrobin, Pyraclostrobin, Trifloxystrobin and one or more inactive excipients.

Moreover, we have found that simultaneous, or combined, application of Dimethomorph with component (A) first fungicide and component (B) second fungicide allows better control of harmful fungi than is possible with the binary mixtures or the individual compounds alone (synergistic mixtures).

According to the present invention there is provided a synergistic and stable fungicide composition comprising from 0.1 to 25% Dimethomorph and 0.1 to 25% component (A) first fungicide selected from Metalaxyl-M, Cymoxanil, Cyazofamid, Fluopicolide and 0.1 to 50% component (B) second fungicide selected from Captan, Chlorothalonil, Fluazinam, Folpet, Mancozeb, Metiram, Propineb, Ziram, Zoxamide, Boscalid, Azoxystrobin, Pyraclostrobin, Trifloxystrobin by weight of active ingredients.

According to another aspect of present invention also relates to process for preparing the novel composition comprising bioactive amounts of Dimethomorph and component (A) first fungicide and component (B) second fungicide with one or more inactive excipients.

DETAILED DESCRIPTION OF THE INVENTION

The term "health of a plant" or "plant health" is defined as a condition of the plant and/or its products. As a result of the improved health, yield, plant vigor, quality and tolerance to abiotic or biotic stress are increased. Noteworthy, the health of a plant when applying the method according to the invention, is increased independently of the fungicidal properties of the active ingredients used because the increase in health is not based upon the reduced pest pressure but instead on complex physiological and metabolic reactions which result for example in an activation of the plant's own natural defense system. As a result, the health of a plant is increased even in the absence of pest pressure.

Accordingly, in an especially preferred embodiment of the method according to the invention, the health of a plant is increased both in the presence and absence of biotic or abiotic stress factors.

The above identified indicators for the health condition of a plant may be interdependent or they may result from each other. An increase in plant vigor may for example result in an increased yield and/or tolerance to abiotic or biotic stress. One indicator for the condition of the plant is the yield. "Yield" is to be understood as any plant product of economic value that is produced by the plant such as grains, fruits in the proper sense, vegetables, nuts, grains, seeds, wood (e.g. in the case of silviculture plants) or even flowers (e.g. in the case of gardening plants, ornamentals). The plant products may in addition be further utilized and/or processed after harvesting.

In an especially preferred embodiment of the invention, the yield of the treated plant is increased.

In another preferred embodiment of the invention, the yield of the plants treated according to the method of the invention, is increased synergistically.

According to the present invention, "increased yield" of a plant, in particular of an agricultural, silvicultural and/or horticultural plant means that the yield of a product of the respective plant is increased by a measurable amount over the yield of the same product of the plant produced under the same conditions, but without the application of the mixture according to the invention.
Increase in yield of treated plants (cereals, pulses, oilseeds, fiber crop, sugar crops, leafy vegetables, tuber crops, fruit crops, flowers, ornamentals etc.). Increase in yield means increased plant weight, increased plant height, increased biomass such as higher overall fresh weight (FW), increased number of flowers per plant, higher grain yield, more tillers or side shoots (branches), larger leaves, increased shoot growth, increased protein content, increased oil content, increased starch content, increased pigment content, Increase in yield due to protection against Insect-pest damage and fungal diseases, Increase in yield due to plant growth regulation, check vegetative growth and increase in reproductive parts of plant, Increase in yield due to more number of tillers, more branches and sub branches, more number of fruits, flowers, and grains size, Increase plant vigor, Increase tolerance to insect-pests and fungal damage, Increase tolerance to the weather stress, moisture stress and heat stress, Prevents lodging in susceptible plants (lodging due to biotic and abiotic factors, like heavy rains, winds, insects and diseases damage.
A further indicator for the condition of the plant is the plant vigor. The plant vigor becomes manifest in several aspects such as the general visual appearance.
In another especially preferred embodiment of the invention, the plant vigor of the treated plant is increased. In another preferred embodiment of the invention, the plant vigor of the plants treated according to the method of the invention is increased synergistically.

Improved plant vigor can be characterized, among others, by the following improved properties of the plant:
• improved vitality of the plant, improved plant growth, improved plant development, improved visual appearance, improved plant stand (less plant verse/lodging), improved emergence, enhanced root growth and/or more developed root system, enhanced nodulation, in particular rhizobial nodulation, bigger leaf blade, bigger size, increased plant weight, increased plant height, increased tiller number, increased number of side shoots, increased number of flowers per plant, increased shoot growth, increased root growth (extensive root system), increased yield when grown on poor soils or unfavorable climate, enhanced photosynthetic activity (e.g. based on increased stomatal conductance and/or increased C02 assimilation rate), increased stomatal conductance, increased C02 assimilation rate, enhanced pigment content (e.g. chlorophyll content), earlier flowering, earlier fruiting, earlier and improved germination, earlier grain maturity, improved self-defence mechanisms, improved stress tolerance and resistance of the plants against biotic and abioticstress factors such as fungi, bacteria, viruses, insects, heat stress, cold stress,drought stress, UV stress and/or salt stress, less non-productive tillers, less dead basal leaves, less input needed (such as fertilizers or water), greener leaves, complete maturation under shortened vegetation periods, less fertilizers needed, less seeds needed, easier harvesting, faster and more uniform ripening, longer shelf-life, longer panicles, delay of senescence, stronger and/or more productive tillers, better extractability of ingredients, improved quality of seeds (for being seeded in the following seasons for seed production), better nitrogen uptake, improved reproduction, reduced production of ethylene and/or the inhibition of its reception by the plant.

The improvement of the plant vigor according to the present invention particularly means that the improvement of any one or several or all of the above mentioned plant characteristics are improved independently of the fungicidal action of the mixture or active ingredients (components). Another indicator for the condition of the plant is the "quality" of a plant and/or its products.

In an especially preferred embodiment of the invention, the quality of the treated plant is increased.

In another preferred embodiment of the invention, the quality of the plants treated according to the method of the invention, is increased synergistically.

According to the present invention, enhanced quality means that certain plant characteristics such as the content or composition of certain ingredients are increased or improved by a measurable or noticeable amount over the same factor of the plant produced under the same conditions, but without the application of the mixtures of the present invention. Enhanced quality can be characterized, among others, by following improved properties of the plant or its product:
• increased nutrient content, increased protein content, increased content of fatty acids, increased metabolite content, increased carotenoid content, increased sugar content, increased amount of essential amino acids, improved nutrient composition, improved protein composition, improved composition of fatty acids, improved metabolite composition, improved carotenoid composition, improved sugar composition, improved amino acids composition, improved or optimal fruit color, improved leaf color, higher storage capacity, higher processability of the harvested products.

Another indicator for the condition of the plant is the plant's tolerance or resistance to biotic and/or abiotic stress factors. Biotic and abiotic stress, especially over longer terms, can have harmful effects on plants. Biotic stress is caused by living organisms while abiotic stress is caused for example by environmental extremes. According to the present invention, "enhanced tolerance or resistance to biotic and/or abiotic stress factors" means that certain negative factors caused by biotic and/or abiotic stress are diminished in a measurable or noticeable amount as compared to plants exposed to the same conditions, but without being treated with a mixture according to the invention and that the negative effects are not diminished by a direct action of the mixture according to the invention on the stress factors, e.g. by its fungicidal or insecticidal action which directly destroys the microorganisms or pests, but rather by a stimulation of the plants' own defensive reactions against said stress factors.

In another preferred embodiment of the invention relates to fungicide composition comprising, as active components,
1) an Dimethomorph fungicide;
2) at least one fungicidal component(A) first fungicide selected from Metalaxyl-M, Cymoxanil, Cyazofamid, Fluopicolide;
3) at least one fungicidal component(B) second fungicide selected from Captan, Chlorothalonil, Fluazinam, Folpet, Mancozeb, Metiram, Propineb, Ziram, Zoxamide, Boscalid, Azoxystrobin, Pyraclostrobin, Trifloxystrobin; and
4) Optionally one or more inactive excipient.

In another preferred embodiment of the invention relates to a method for controlling harmful fungi using mixtures of a Dimethomorph and component (A) first fungicide and component (B) second fungicide.

In another preferred embodiment of the invention relates to a process for preparation mixtures of a Dimethomorph and component (A) first fungicide and component (B) second fungicide for preparing such three way mixtures, and also to compositions comprising these mixtures.

In another preferred embodiment of the invention fungicide composition of present invention can be in form of Suspension Concentrate (SC), Suspo emulsion (SE), Water dispersible granules (WG/WDG), Emulsifiable concentrate (EC), Wettable Powders (WP), Oil dispersion (OD), capsule suspension (CS), Zean Concentrate (ZC). Preferably the fungicidal composition of the present invention may be in the form of Suspension Concentrate (SC), Suspo emulsion (SE). In a preferred embodiment, the composition is in the form of water-dispersible granules (WDG), suspension concentrate (SC) and wettable powder (WP).

The present invention relates to fungicidal composition comprising Dimethomorph and component (A) first fungicide selected from Metalaxyl-M, Cymoxanil, Cyazofamid, Fluopicolide and component (B) second fungicide selected from Captan, Chlorothalonil, Fluazinam, Folpet, Mancozeb, Metiram, Propineb, Ziram, Zoxamide, Boscalid, Azoxystrobin, Pyraclostrobin, Trifloxystrobin in ratios by weight of the active ingredients are 0.1 to 25% Dimethomorph; 0.1 to 25% component(A) first fungicide selected and 0.1 to 50% component(B) second fungicide.

The present invention relates to fungicidal composition comprising ratios by weight:

Active Ingredient I Active Ingredient II
Component (A) Active Ingredient III
Component (B)


Dimethomorph











Metalaxyl-M
Cyamoxanil
Cyazofamid
Fluopicolide








Captan
Chlorothalonil
Fluazinam
Folpet
Mancozeb
Metiram
Propineb
Ziram
Zoxamide
Boscalid
Azoxystrobin
Pyraclostrobin
Trifloxystrobin
0.1 to 25% 0.1 to 25% 0.1 to 50%

List of inactive agent which may be used in the present invention are listed below but not limited to:
Inactive excipients used in SC formulation
Excipients Function
Acrylic graft copolymer, Alkylated naphthalene sulfonate, sodium salt, Sodium salt of naphthalene sulfonate condensate, Sodium ligno sulfonate, Polyacrylate polymer sodium salt Dispersing agent
Polyalkoxy alkyl ether, Ethylene oxide/propylene oxide block copolymer Dispersing agent, Emulsifier
Polyarylphenyl ether phosphate, Ethoxylated Fatty Alcohol Dispersing agent & Wetting agent
Trisiloxane ethoxylate Wetting agent & Spreader
Sodium dioctyl sulfosuccinate Wetting agent & Emulsifier
Silicone antifoam emulsion Antifoam
Polysaccharide Rheology Modifier, Thickner
Glycol (Monoethylene glycol, Diethylene glycol, Polypropylene glycol, Polyethylene glycol), Glycerene Antifreezing Agent
1,2-benzisothiazolin-3-one, Sodium benzoate, 2-bromo-2-nitropropane-1,3-diol, Formaldehyde, Sodium o-phenylphenate, 5-chloro-2-methyl-4-isothiazolin-3-one & 2-methyl-4-isothiazolin-3-one Biocide/Preservative
Aluminum Magnesium Silicate Suspending aid
Silicon dioxide Suspending aid, Anticaking agent
Polydimethyl siloxane Antifoaming agent
Organo modified Trisiloxane Spreader
Poly glycerol ester Sticker, Penetrant
Poly ether Deposition aid

Inactive excipients used in WG and WP formulations
Excipients Function
Sodium salt of naphthalene sulfonate condensate, Modified polyacrylate copolymer, Sodium polycarboxylate, Sodium Ligno sulfonate, Alkyl naphthalene sulfonate condensate, sodium salt, Dispersing Agent
Polyacrylate co-polymer, Polyacrylate polymer sodium salt Dispersing Agent, Stabilizer, Emulsifier
Alcohol block co-polymer Co-dispersant
Blend of naphthalene sulfonate condensate & phenol sulfonate condensate Wetting Agent & Dispersing Agent
blend of naphthalene sulfonate condensate, Sodium dodecylbenzene sulfonate, Sodium lauryl sulfate, Blend of alkyl naphthalene sulfonate and anionic wetting agent, Sodium n-butyl naphthalene sulfonate, Sodium isopropyl naphthalene sulfonate, Blend of sodium alkyl aryl sulfonates Wetting Agent
Corn starch Binder, Filler
Silicon dioxide Suspending agent, Anti-caking Agent, Filler
Urea formaldehyde resin / Urea-methanol Anti-caking agent
Lactose, China clay/ Kaolin, Titanium dioxide, Talcum powder, Diatomaceous earth Filler
Polyvinylpyrrolidone Binder
Silicone antifoam emulsion Defoaming agent
Fatty amine ethoxylate Wetting agent, Dispersing agent, Stabilizers, Sanitizers, Defoaming agent

The fungicidal composition of Dimethomorph and component(A) first fungicide and component(B) second fungicide is effective for management of mixed infection of fungal diseases in following crops Cotton (Gossypium spp.), Jute (Corchorusoliotorus), Paddy (Oryza sativa), Wheat (Triticumaestavum), Barley (Hordeumvulgare), Maize (Zea mays), Sorghum (Sorghum bicolor), Ragi (Eleusinecoracana), Pearl millet (Pennisetumglaucum), Sugarcane (Saccharumofficinarum) , Sugarbeet (Beta vulgaris), Soybean (Glycin max), Peanut (Arachishypogaea), Sunflower (Helianthus annuus) , Mustard (Brassica juncea), Rape seed (Brassica napus), Linseed (Linumusitatissimum), Sesame (Sesamumindicum), Castor (Ricinuscommunis), Green gram (Vigna radiate), Black gram (Vignamungo), Chickpea (Ciceraritinum), Cowpea (Vignaunguiculata), Redgram (Cajanuscajan), Frenchbean (Phaseolus vulgaris), Indian bean (Lablab purpureus), Horse gram (Macrotylomauniflorum), Field pea (Pisumsativum), Cluster bean (Cyamopsistetragonoloba), Lentils (Lens culinaris), Brinjal (Solanummelongena), Cabbage (Brassica oleracea var. capitata), Cauliflower (Brassica oleracea var. botrytis), Okra (Abelmoschusesculentus) , Onion (Allium cepa L.), Tomato (Solanumlycopersicun) , Potato (Solanumtuberosum) , Sweet potato (Ipomoea batatas), Chilly (Capsicum annum), Garlic (Allium sativum), Cucumber (Cucumissativus), Muskmelons (Cucumismelo), Watermelon (Citrulluslanatus), Bottle gourd (Lagenariasiceraria), Bitter gourd (Momordicacharantia), Radish (Raphanussativus), Carrot (Dacuscarota subsp. sativus), Turnip (Brassica rapasubsprapa), Apple (Melusdomestica), Banana (Musa spp.), Citrus groups (Citrus spp.), Grape (Vitisvinifera), Guava (Psidiumguajava), Litchi (Litchi chinensis), Mango (Mangiferaindica), Papaya (Carica papaya), Pineapple (Ananascomosus), Pomegranate (Punicagranatum) , Sapota (Manilkarazapota), Tea (Camellia sinensis), Coffea (Coffea Arabica), Turmeric (Curcuma longa), Ginger (Zingiberofficinale), Cumin (Cuminumcyminum), Fenugreek (Trigonellafoenum-graecum), Fennel (Foeniculumvulgare), Coriander (Coriandrumsativum), Ajwain (Trachyspermumammi), Psyllium (Plantago ovate), Black Pepper (Piper nigrum), Stevia (Stevia rebaudiana), Safedmusli (Chlorophytumtuberosum), Drum stick (Moringaoleifera), Coconut (Coco nucifera), Mentha ( Mentha spp.), Rose (Rosa spp.), Jasmine (Jasminum spp.), Marigold ( Tagetes spp.), Common daisy (Bellisperennis), Dahlia (Dahlia hortnesis), Gerbera ( Gerbera jamesonii), Carnation (Dianthus caryophyllus)

The fungicidal composition of Dimethomorph and component(A) first fungicide and component(B) second fungicide is according to the invention have very good fungicidal properties and can beemployed for controlling phytopathogenic fungi such as Ascomycetes, Basidiomycetes, Chytridiomycetes, Deuteromycetes, Oomycetes, Plasmodiophoromycetes, Zygomycetes, and the like.Examples which may be mentioned, but not by limitation, are some pathogens of fungal diseaseswhich come under the above generic terms: Diseases caused by pathogens causing powdery mildew such as, for example, Blumeria species such as, for example, Blumeriagraminis; Podosphaera species such as, for example, Podosphaeraleucotricha;Sphaerotheca species such as, for example, Sphaerothecafuliginea; Uncinula species such as, for example, Uncinulanecator; Leveillula species such as, for example Leveillulataurica, Erysiphe species such as for example Erysiphepolygoni, diseases caused by pathogens of rust diseases such as, for example, Gymnosporangium species such as, for example, Gymnosporangiumsabinae, Hemileia species such as, for example, Hemileiavastatrix; Phakopsora species such as, for example, Phakopsorapachyrhizi and Phakopsorameibomiae; Puccinia species such as, for example, Pucciniagraminis, Pucciniarecondita or Pucciniatriticina, Pucciniastriiformis; Uromyces species such as, for example, Uromycesphaseoli; diseases caused by pathogens of smut diseases such as, for example, Sporisorium species such as , for example, Sporisoriumscitamineum; Ustilago species such as, for example Ustilagomaydis, Tilletia species such as for example Tilletiatritici, Ustilaginoidea species such as , for example Ustilaginoideavirens, diseases caused by pathogens of ergot diseases such as, for example Claviceps species, Clavicepspurpurea; leaf spot, leaf blight and leaf wilt diseases caused by, for example, Alternaria species such as, for example, Alternariasolani; Cercospora species such as, for example, Cercosporabeticola; Cladiosporum species such as, for example, Cladiosporiumcucumerinum; Cochliobolus species such as, for example, Cochliobolussativus (conidial form: Drechslera, syn: Helminthosporium); Colletotrichum species such as, for example, Colletotrichumlindemuthanium; Cycloconium species such as, for example, Cycloconiumoleaginum; Diaporthe species such as, for example, Diaporthecitri; Elsinoe species such as, for example, Elsinoefawcettii; Gloeosporium species such as, for example, Gloeosporiumlaeticolor; Glomerella species such as, for example, Glomerellacingulata; Guignardia species such as, for example, Guignardiabidwelli; Leptosphaeria species such as, for example, Leptosphaeriamaculans; Magnaporthe species such as, for example, Magnaporthegrisea;Mycosphaerella species such as, for example, Mycosphaerellagraminicola; Phaeosphaeria species such as, for example, Phaeosphaerianodorum; Pyrenophora species such as, for example, Pyrenophorateres;Ramularia species such as, for example, Ramulariacollo-cygni; Rhynchosporium species such as, for example, Rhynchosporiumsecalis; Septoria species such as, for example, Septoriaapii;Typhula species such as, for example, Typhulaincarnata; Venturia species such as, for example, Venturiainaequalis; root and stalk diseases, caused by, for example, Corticium species such as, for example, Corticiumgraminearum; Fusarium species such as, for example, Fusariumoxysporum;Gaeumannomyces species such as, for example, Gaeumannomycesgraminis; Rhizoctonia species such as, for example, Rhizoctoniasolani; Tapesia species such as, for example, Tapesiaacuformis;Thielaviopsis species such as, for example, Thielaviopsisbasicola; ear and panicle diseases (including maize cobs), caused by, for example, Alternaria species such as, for example, Alternaria spp.;Aspergillus species such as, for example, Aspergillusflavus; Cladosporium species such as, for example, Cladosporium spp.; Claviceps species such as, for example, Clavicepspurpurea;
Fusarium species such as, for example, Fusariumculmorum; Gibberella species such as, for example, Gibberellazeae; Monographella species such as, for example, Monographellanivalis;Smut diseases caused by, for example, Sphacelotheca species such as, for example, Sphacelothecareiliana; Tilletia species such as, for example, Tilletia caries; Urocystis species such as, for example, Urocystisocculta; Ustilago species such as, for example, Ustilagonuda; fruit rot caused by, for example, Aspergillus species such as, for example, Aspergillusflavus; Botrytis species such as, for example, Botrytis cinerea; Penicillium species such as, for example, Penicilliumexpansum; Sclerotinia species such as, for example, Sclerotiniasclerotiorum; Verticilium species such as, for example, Verticiliumalboatrum; seed- and soil-borne rots and wilts, and seedling diseases, caused by, for example, Fusarium species such as, for example, Fusariumculmorum; Phytophthora species such as, for example, Phytophthoracactorum; Pythium species such as, for example, Pythiumultimum; Rhizoctonia species such as, for example, Rhizoctoniasolani; Sclerotium species such as, for example, Sclerotiumrolfsii; cankers, galls and witches' broom diseases, caused by, for example, Nectria species such as, for example, Nectriagalligena; wilts caused by, for example, Monilinia species such as, for example, Monilinialaxa; deformations of leaves, flowers and fruits, caused by, for example, Taphrina species such as, for example, Taphrinadeformans; degenerative diseases of woody species, caused by, for example, Esca species such as, for example, Phaemoniellaclamydospora; flower and seed diseases, caused by, for example, Botrytis species such as, for example, Botrytis cinerea; diseases of plant tubers caused by, for example, Rhizoctonia species such as, for example, Rhizoctoniasolani; diseases caused by bacterial pathogens such as, for example, Xanthomonas species such as, for example, Xanthomonascampestrispv. oryzae; Pseudomonas species such as, for example, Pseudomonas syringaepv. lachrymans; Erwinia species such as, for example, Erwiniaamylovora etc.

The present inventions can be widely used to protect the agricultural, horticultural and forest plant species from the diseases caused by pathogens from the group of the Oomycetes, such as, for example, Pythium species such as, Pythium blight (high temperature) by Pythiumaphanidermatum, Pythium blight (low temperature) by Pythiumgraminicola, Pythiumultimum, Pythium damping off diseases in nurseries of tobacco, tomato, chillies, egg-plant; Phytophthora species such as, Phytophthorainfestans causing late blight in potato and tomato, Gummosis and Root rot in citrus by Phytophthora spp., seed rot, seedling blight, fruit rot in chilli and cucurbits by Phytophthoracapsici, downy mildew of cucurbits by Pseudoperonosporacubensis, downy mildew of millets by Sclerosporagraminicola, downy mildew of maize by Peronosclerosporasroghi, white rust of crucifers by Albugo candida, downy mildew of grape by Plasmoparaviticola.

A per one embodiment, the synergistic fungicidal composition of present invention is effective for disease selected from Pythium blight (high temperature) by Pythium aphanidermatum, Pythium blight (low temperature) by Pythium graminicola, Pythium ultimum, Pythium damping off diseases in nurseries of tobacco, tomato, chillies, egg-plant; Phytophthora species such as, Phytophthora infestans causing late blight in potato and tomato, Gummosis and Root rot in citrus by Phytophthora spp., seed rot, seedling blight, fruit rot in chilli and cucurbits by Phytophthora capsici, downy mildew of cucurbits by Pseudoperonospora cubensis, downy mildew of millets by Sclerospora graminicola, downy mildew of maize by Peronosclerospora sroghi, white rust of crucifers by Albugo candida, downy mildew of grape by Plasmopara viticola, leaf spot, leaf blight caused by Alternaria solani; Cercospora species, Colletotrichum species, Septoria species in fruits and vegetables

The fungicidal composition of the present invention in addition to Dimethomorph and component(A) first fungicide and component(B) second fungicide further comprises inactive excipients including but not limited to dispersant, anti-freezing agent, anti-foam agent, wetting agent, suspension aid, anti-microbial agent, thickener, quick coating agent or sticking agents (also referred to as “stickers” or “binders”) and buffering agent.

Surfactants that are used as dispersants have the ability to adsorb strongly onto a particle surface and provide a charged or steric barrier to re-aggregation of particles. The most commonly used surfactants are anionic, non-ionic, or mixtures of the two types. For wettable powder formulations, the most common dispersants are sodium lignosulphonates. For suspension concentrates, very good adsorption and stabilization are obtained using polyelectrolytes, such as sodium naphthalene sulphonate formaldehyde condensates. Tristyrylphenolethoxylate phosphate esters are also used. Nonionics such as alkylarylethylene oxide condensates and EO-PO block copolymers are sometimes combined with anionics as dispersants for suspension concentrates. In recent years, new types of very high molecular weight polymeric surfactants have been developed as dispersants. These have very long hydrophobic ‘backbones’ and a large number of ethylene oxide chains forming the ‘teeth’ of a ‘comb’ surfactant. These high molecular weight polymers can give very good long-term stability to suspension concentrates because the hydrophobic backbones have many anchoring points onto the particle surfaces. Examples of dispersants used herein include but not limited to sodium lignosulphonates; sodium naphthalene sulphonate formaldehyde condensates; tristyrylphenolethoxylate phosphate esters; aliphatic alcohol ethoxylates; alky ethoxylates; EO-PO block copolymers; and graft copolymers or mixtures thereof.

Anti-freezing agent as used herein can be selected from the group consisting of polyethylene glycols, methoxypolyethylene glycols, polypropylene glycols, polybutylene glycols, glycerin and ethylene glycol.

Water-based formulations often cause foam during mixing operations in production. In order to reduce the tendency to foam, anti-foam agents are often added either during the production stage or before filling into bottles. Generally, there are two types of anti-foam agents, namely silicones and non-silicones. Silicones are usually aqueous emulsions of dimethyl polysiloxane while the non-silicone anti-foam agents are water-insoluble oils, such as octanol and nonanol, or silica. In both cases, the function of the anti-foam agent is to displace the surfactant from the air-water interface.

Examples of wetting agents used in wettable powder, suspension concentrate, and water-dispersible granule formulations include but not limited to sodium lauryl sulphate; sodium dioctylsulphosuccinate; alkyl phenol ethoxylates; and aliphatic alcohol ethoxylates or mixtures thereof.

Suspension aid in the present description denotes a natural or synthetic, organic or inorganic material with which the active substance is combined in order to facilitate its application to the plant, to the seeds or to the soil. This carrier is hence generally inert, and it must be agriculturally acceptable, in particular to the plant being treated. The carrier may be solid (clays, natural or synthetic silicates, silica, resins, waxes, solid fertilizers, and the like or mixtures thereof) or liquid (water, alcohols, ketones, petroleum fractions, aromatic or paraffinic hydrocarbons, chlorinated hydrocarbons, liquefied gases, and the like or mixtures thereof).

Biocides / Microorganisms cause spoilage of formulated products. Therefore anti-microbial agents are used to eliminate or reduce their effect. Examples of such agents include, but are not limited to: propionic acid and its sodium salt; sorbic acid and its sodium or potassium salts; benzoic acid and its sodium salt; p-hydroxy benzoic acid sodium salt; methyl p-hydroxy benzoate; and biocide such as sodium benzoate, 1,2-benzisothiazoline-3-one, 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, potassium sorbate, parahydroxy benzoates or mixtures thereof.

Thickening, gelling, and anti-settling agents generally fall into two categories, namely water-insoluble particulates and water-soluble polymers. It is possible to produce suspension concentrate formulations using clays and silicas. Examples of these types of materials, include, but are limited to, montmorillonite, e.g. bentonite; magnesium aluminum silicate; and attapulgite. Water-soluble polysaccharides have been used as thickening-gelling agents for many years. The types of polysaccharides most commonly used are natural extracts of seeds and seaweeds are synthetic derivatives of cellulose or mixtures thereof. Examples of these types of materials include, but are not limited to, guar gum; locust bean gum; carrageenam; xanthan gum; alginates; methyl cellulose; sodium carboxymethyl cellulose (SCMC); hydroxyethyl cellulose (HEC) or mixtures thereof. Other types of anti-settling agents are based on modified starches, polyacrylates salts, polyvinyl alcohol and polyethylene oxide or mixtures.

As per one embodiment, the dispersing agent is selected from guar gum; locust bean gum; carrageenam; xanthan gum; alginates; methyl cellulose; sodium carboxymethyl cellulose (SCMC); hydroxyethyl cellulose (HEC), modified starches, polyacrylates salts, polyvinyl alcohol and polyethylene oxide or mixtures thereof. The dispersing agent is present in the range from 0.2 to 20% w/w, preferably in the range from 0.5 to 5 % w/w.

The quick coating agent can be a conventionally available sticker, for example polyesters, polyamides, poly- carbonates, polyurea and polyurethanes, acrylate polymers and copolymers, styrene copolymers, butadiene copolymers, polysaccharides such as starch and cellulose derivatives, vinylalcohol, vinylacetate and vinylpyrrolidone polymers and copolymers, polyethers, epoxy, phenolic and melamine resins, polyolefins and define copolymersand mixtures thereof. Examples of preferred polymers are acrylate polymers such as poly(methacrylate), poly(ethyl methacrylate), poly(methylmethacrylate), acrylate copoylmers and styrene-acrylic copolymers as defined herein below, poly(styrene-co maleic anhydride), cellulosic polymers such as ethyl cellulose, cellulose acetate, cellulose acetatebutyrate, acetylated mono, di, and triglycerides, poly(vinylpyrrolidone), vinyl acetate polymers and copolymers, poly(alkylene glycol), styrene butadiene copolymers, poly(orthoesters), alkyd resins, and mixtures of two or more of these. Polymers that are biodegradable are also useful in the present invention. As used herein, a polymer is biodegradable if is not water soluble, but is degraded over a period of several weeks when placed in an application environment. Examples of biodegradable polymers that are useful in the present invention include biodegradable polyesters, starch, polylactic acid starch blends, polylactic acid, poly(lactic acid-glycolic acid) copolymers, polydioxanone, cellulose esters, ethyl cellulose, cellulose acetate butyrate, starch esters, starch esteraliphatic polyester blends, modified corn starch, polycaprolactone, poly(namylmethacrylate), wood rosin, polyanhydrides, polyvinylalcohol, polyhydroxybutyratevalerate, biodegradable aliphatic polyesters, and polyhydroxybutyrate or mixtures thereof.

Buffering agent as used herein is selected from group consisting of calcium hydroxyapatite, Potassium Dihydrogen Phosphate, Sodium Hydroxide, carbonated apatite, calcium carbonate, sodium bicarbonate, tri-calcium phosphate, calcium phosphates, carbonated calcium phosphates, amine monomers, lactate dehydrogenase and magnesium hydroxide.

The solvent for the formulation of the present invention may include water, water-soluble alcohols and dihydroxy alcohol ethers. The water-soluble alcohol which can be used in the present invention may be lower alcohols or water-soluble macromolecular alcohols. The term "lower alcohol", as used herein, represents an alcohol having 1-4 carbon atoms, such as methanol, ethanol, n-propanol, isopropanol, n-butanol, tert-butanol, etc. Macromolecular alcohol is not limited, as long as it may be dissolved in water in a suitable amount range, e.g., polyethylene glycol, sorbitol, glucitol, etc. The examples of suitable dihydroxyalcohol ethers used in the present invention may be dihydroxy alcohol alkyl ethers or dihydroxy alcohol aryl ethers. The examples of dihydroxy alcohol alkyl ether include ethylene glycol methyl ether, diethylene glycol methyl ether, propylene glycol methyl ether, dipropylene glycol methyl ether, ethylene glycol ethyl ether, diethylene glycol ethyl ether, propylene glycol ethyl ether, dipropylene glycol ethyl ether, etc. The examples of dihydroxy alcohol aryl ethers include ethylene glycol phenyl ether, diethylene glycol phenyl ether, propylene glycol phenyl ether, dipropylene glycol phenyl ether, and the like. Any of the above mentioned solvent can be used either alone or in combination thereof.

The water dispersible granules (WDG) or wettable powder (WP) can be applied after disintegration and dispersion in water. Water dispersible granules can be formed by a) agglomeration, b) spray drying, or c) extrusion techniques.

WDG formulations offer a number of advantages in packaging, ease of handling and safety. The WDG are preferably of uniform size and which are free flowing, low dusting and readily disperse in water to form a homogenous solution of very small particles which may pass through conventional spray nozzles. Ideally WDG formulations when dispersed in water under gentle agitation for five minutes have residues of less than 0.01% on a 150 µm sieve screen and less than 0.5% on a 53 µm screen. The granules can usually be measured accurately by volume which is convenient for the end user.

The SC formulation can be prepared by below described method;
STEP-I: Adding anti-microbial agent and gum in water under continuous stirring followed by slow addition. Continuing stirring until homogeneous dispersion is formed.
STEP-II: Mixing anti-freezing agent, dispersant, wetting agent, anti-microbial agent and anti-foaming agent in water for 30 minute until homogeneous solution is formed. Finally add Dimethomorph and component (A) first fungicide and component (B) second fungicide slowly under continuous stirring at 30 minute till homogeneous dispersion is obtained. Milling the slurry through bead mill until required particle size is achieved.
Step-III: Adding rest of water, anti-foaming agent and gum solution under continuous stirring to get desired viscosity of the suspension. Continue stirring for about 4 hr to obtain homogeneous formulation.

The process for preparing the present novel formulation can be modified accordingly by any person skilled in the art based on the knowledge of the manufacturing the formulation. However all such variation and modification is still covered by the scope of present invention.

EXAMPLES

Example 1: Dimethomorph 16%+Metalaxyl-M 2.5%+Mancozeb 45% WG
Chemical Composition Percent (% w/w)
Dimethomorph a.i. 16.0
Metalaxyl-M a.i. 2.5
Mancozeb a.i. 45.0
Alkylated naphthalene sulfonate, sodium salt 7.0
Polyacrylate polymer sodium salt 2.0
Sodium alkyl naphthalene sulfonate blend 1.0
Silicone antifoam 1.0
Lactose 5.0
Corn starch 8.0
China Clay QS
TOTAL 100.0

Procedure:
Step 1 Charge the required quantity of filler, wetting agent, dispersing agent, and suspending agent, & technical in premixing blender for homogenization for 30 minutes.
Step 2 Pre-blended material is then grinded through Jet mill/ air classifier mills. Finely grinded material is blended in post blender till it becomes homogeneous. (for approx 1.5 hr)
Step 3 Finely grinded powder is mixed with required quantity of water to form extrudable dough.
Step 4 Dough is passed through extruder to get granules of required size.
Step 5 Wet granules are passed through Fluidized bed drier and further graded using vibrating screens.
Step 6 Final product is sent for QC approval.
Step 7 After approval material is packed in required pack sizes.

Storage stability study-Dimethomorph 16%+Metalaxyl-M 2.5%+Mancozeb 45% WG
Parameters Specification (In house) Initial Stability (for 14 days)
At 54±2 0C At 0±2 0C
Description Off white colour granules Complies Complies Complies
Dimethomorph content percent by mass 15.20 to 16.80 16.15 16.11 16.15
Metalaxyl-M content percent by mass 2.375 to 2.75 2.54 2.52 2.54
Mancozeb content percent by mass 42.75 to 47.25 45.22 45.17 45.21
Dimethomorph suspensibility percent min. 70 96.58 95.54 96.11
Metalaxyl-M suspensibility percent min. 70 95.14 92.95 94.58
Mancozeb suspensibility percent min. 70 87.85 85.01 85.81
pH range (1% aq. Suspension) 5.5 to7.5 7.09 7.07 7.09
Wettability sec. max. 60 10 11 10
Wet Sieve(45 micron ) percent by mass min. 98.5 99.6 99.3 99.5
Bulk density (g/ml) 0.45 to 0.75 0.49 0.49 0.49
Moisture content percent by mass max. Max. 2.0% 1.2 1 1

Room Temperature storage
Parameters Specification (In house) Study Duration
1 month 6 month 12 months 24 months
Description Off white colour granules Complies Complies Complies Complies
Dimethomorph content percent by mass 15.20 to 16.80 16.14 16.13 16.13 16.12
Metalaxyl-M content percent by mass 2.375 to 2.75 2.54 2.54 2.54 2.52
Mancozeb content percent by mass 42.75 to 47.25 45.21 45.19 45.18 45.17
Dimethomorph suspensibility percent min. 70 96.95 96.41 95.32 95.53
Metalaxyl-M suspensibility percent min. 70 94.41 94.22 93.87 93.75
Mancozeb suspensibility percent min. 70 85.76 85.48 85.39 84.87
pH range (1% aq. Suspension) 5.5 to7.5 7.2 7.2 7.2 7.1
Wettability sec. max. 60 10 10 11 11
Wet Sieve(45 micron ) percent by mass min. 98.5 99.6 99.5 99.5 99.4
Bulk density (g/ml) 0.45 to 0.75 0.49 0.49 0.49 0.49
Moisture content percent by mass max. Max. 2.0% 1.2 1.2 1.1 1

Example 2: Dimethomorph 16%+Metalaxyl-M 2.5%+Mancozeb 45% WP
Chemical Composition Percent
(% w/w)
Dimethomorph a.i. 16.0
Metalaxyl-M a.i. 2.5
Mancozeb a.i. 45.0
Alkylated naphthalene sulfonate, sodium salt 7.0
Polyacrylate polymer sodium salt 2.0
Sodium alkyl naphthalene sulfonate blend 1.0
Silicone antifoam 1.0
Precipitated Silica 5.0
China Clay QS
TOTAL 100.0

Procedure: As per Example 1
Storage stability study-Dimethomorph 16%+Metalaxyl-M 2.5%+Mancozeb 45% WP
Parameters Specification (In house) Initial Stability (for 14 days)
At 54±2 0C At 0±2 0C
Description Off white colour Complies Complies Complies
Dimethomorph content percent by mass 15.20 to 16.80 16.17 16.10 16.16
Metalaxyl-M content percent by mass 2.375 to 2.75 2.56 2.54 2.56
Mancozeb content percent by mass 42.75 to 47.25 45.25 45.18 45.24
Dimethomorph suspensibility percent min. 70 98.52 97.48 98.45
Metalaxyl-M suspensibility percent min. 70 97.65 95.85 97.40
Mancozeb suspensibility percent min. 70 87.95 87.02 87.55
pH range (1% aq. Suspension) 5.5 to7.5 7.1 7.1 7.1
Wettability sec. max. 60 10 11 10
Wet Sieve(45 micron ) percent by mass min. 98.5 99.6 99.3 99.5
Bulk density (g/ml) 0.45 to 0.75 0.48 0.48 0.48
Moisture content percent by mass max. Max. 2.0% 1.6 1.2 1

Room temperature storage
Parameters Specification (In house) Study Duration
1 month 6 month 12 months 24 months
Description Off white colour Complies Complies Complies Complies
Dimethomorph content percent by mass 15.20 to 16.80 16.17 16.15 16.14 16.12
Metalaxyl-M content percent by mass 2.375 to 2.75 2.55 2.55 2.55 2.54
Mancozeb content percent by mass 42.75 to 47.25 45.25 45.25 45.24 45.21
Dimethomorph suspensibility percent min. 70 98.48 98.41 98.35 97.95
Metalaxyl-M suspensibility percent min. 70 97.55 97.41 96.98 96.55
Mancozeb suspensibility percent min. 70 87.85 87.61 86.58 86.25
pH range (1% aq. Suspension) 5.5 to7.5 7.1 7.1 7.1 7.1
Wettability sec. max. 60 10 10 11 11
Wet Sieve(45 micron ) percent by mass min. 98.5 99.6 99.5 99.5 99.4
Bulk density (g/ml) 0.45 to 0.75 0.48 0.48 0.48 0.48
Moisture content percent by mass max. Max. 2.0% 1.6 1.6 1.4 1.2

Example 3: -Dimethomorph 25%+Metalaxyl-M 5%+Boscalid 6.25% WG
Chemical Composition Percent (% w/w)
Dimethomorph a.i. 25.0
Metalaxyl-M a.i. 5.0
Boscalid a.i. 6.25
Alkylated naphthalene sulfonate, sodium salt 7.0
Polyacrylate polymer sodium salt 2.0
Sodium alkyl naphthalene sulfonate blend 1.0
Silicone antifoam 1.0
Lactose 5.0
Corn starch 10.0
China Clay QS
TOTAL 100.0

Procedure: As per Example 1
Storage stability study-Dimethomorph 25%+Metalaxyl-M 5%+Boscalid 6.25% WG
Parameters Specification (In house) Initial Stability (for 14 days)
At 54±2 0C At 0±2 0C
Description Off white colour granules Complies Complies Complies
Dimethomorph content percent by mass 23.75 to 26.25 25.24 25.14 25.20
Metalaxyl-M content percent by mass 4.75 to 5.50 5.12 5.06 5.10
Boscalid content percent by mass 5.94 to 6.87 6.36 6.18 6.30
Dimethomorph suspensibility percent min. 70 95.50 94.30 95.46
Metalaxyl-M suspensibility percent min. 70 96.58 94.31 96.22
Boscalid suspensibility percent min. 70 95.15 94.37 95.07
pH range (1% aq. Suspension) 5.5 to7.5 7.1 7.1 7.1
Wettability sec. max. 60 10 11 10
Wet Sieve (45 micron ) percent by mass min. 98.5 99.6 99.3 99.5
Bulk density (g/ml) 0.45 to 0.75 0.48 0.48 0.48
Moisture content percent by mass max. Max. 2.0% 1.6 1.2 1

Room temperature storage
Parameters Specification (In house) Study Duration
1 month 6 month 12 months 24 months
Description Off white colour granules Complies Complies Complies Complies
Dimethomorph content percent by mass 23.75 to 26.25 25.24 25.22 25.20 25.18
Metalaxyl-M content percent by mass 4.75 to 5.5 5.11 5.10 5.10 5.08
Boscalid content percent by mass 5.94 to 6.87 6.35 6.32 6.32 6.30
Dimethomorph suspensibility percent min. 70 95.46 95.38 94.88 94.45
Metalaxyl-M suspensibility percent min. 70 96.38 96.25 95.55 94.88
Boscalid suspensibility percent min. 70 95.05 94.90 94.75 94.50
pH range (1% aq. Suspension) 5.5 to7.5 7.1 7.1 7.1 7.1
Wettability sec. max. 60 10 10 11 11
Wet Sieve (45 micron ) percent by mass min. 98.5 99.6 99.5 99.5 99.4
Bulk density (g/ml) 0.45 to 0.75 0.48 0.48 0.48 0.48
Moisture content percent by mass max. Max. 2.0% 1.6 1.6 1.4 1.2

Example 4: Dimethomorph 16%+Cyazofamid 5%+Mancozeb 50% WG
Chemical Composition Percent (% w/w)
Dimethomorph a.i. 16.0
Cyazofamid a.i. 5.0
Mancozeb a.i. 50.0
Alkylated naphthalene sulfonate, sodium salt 6.0
Polyacrylate polymer sodium salt 2.0
Sodium alkyl naphthalene sulfonate blend 1.0
Silicone antifoam 1.0
Corn starch 10.0
China Clay QS
TOTAL 100.0

Procedure: As per example 1
Storage stability study-Dimethomorph 16%+Cyazofamid 5%+Mancozeb 50% WG
Parameters Specification (In house) Initial Stability
(for 14 days)
At
54±2 0C At
0±2 0C
Description Off white colour granules Complies Complies Complies
Dimethomorph content percent by mass 15.20 to 16.80 16.22 16.15 16.21
Cyazofamid content percent by mass 4.75 to 5.5 5.25 5.11 5.22
Mancozeb content percent by mass 47.5 to 52.5 50.55 50.17 50.52
Dimethomorph suspensibility percent min. 70 95.90 94.40 95.76
Cyazofamid suspensibility percent min. 70 96.91 94.31 96.50
Mancozeb suspensibility percent min. 70 95.15 94.37 95.09
pH range (1% aq. Suspension) 5.5 to7.5 7.1 7.1 7.1
Wettability sec. max. 60 10 11 10
Wet Sieve(45 micron) percent by mass min. 98.5 99.6 99.3 99.5
Bulk density (g/ml) 0.45 to 0.75 0.49 0.49 0.49
Moisture content percent by mass max. Max. 2.0% 1.6 1.2 1

Room temperature storage
Parameters Specification (In house) Study Duration
1 month 6 month 12 months 24 months
Description Off white colour granules Complies Complies Complies Complies
Dimethomorph content percent by mass 15.20 to 16.80 16.21 16.20 16.18 16.15
Cyazofamid content percent by mass 4.75 to 5.5 5.24 5.23 5.21 5.15
Mancozeb content percent by mass 47.5 to 52.5 50.54 50.52 50.46 50.23
Dimethomorph suspensibility percent min. 70 95.86 95.58 94.98 94.89
Cyazofamid suspensibility percent min. 70 96.85 96.25 95.55 94.76
Mancozeb suspensibility percent min. 70 95.07 94.87 94.75 94.67
pH range (1% aq. Suspension) 5.5 to7.5 7.1 7.1 7.1 7.1
Wettability sec. max. 60 10 10 11 11
Wet Sieve(45 micron ) percent by mass min. 98.5 99.6 99.5 99.5 99.4
Bulk density (g/ml) 0.45 to 0.75 0.49 0.49 0.49 0.49
Moisture content percent by mass max. Max. 2.0% 1.6 1.6 1.4 1.2

Example 5: Dimethomorph 25%+Cyazofamid 7.8%+Pyraclostrobin 10% WG
Chemical Composition Percent (% w/w)
Dimethomorph a.i. 25.0
Cyazofamid a.i. 7.8
Pyraclostrobin a.i. 10.0
Alkylated naphthalene sulfonate, sodium salt 6.0
Polyacrylate polymer sodium salt 2.0
Sodium Lauryl Sulfate 3.0
Silicone antifoam 1.0
Corn starch 8.0
China Clay QS
TOTAL 100.0

Procedure: As per Example 1
Storage stability study-Dimethomorph 25%+Cyazofamid 7.8%+Pyraclostrobin 10% WG
Parameters Specification (In house) Initial Stability (for 14 days)
At 54±2 0C At 0±2 0C
Description Off white colour granules Complies Complies Complies
Dimethomorph content percent by mass 23.75 to 26.25 25.24 25.16 25.22
Cyazofamid content percent by mass 7.41 to 8.58 7.88 7.82 7.86
Pyraclostrobin content percent by mass 9.5 to 10.5 10.20 10.12 10.18
Dimethomorph suspensibility percent min. 70 95.85 94.22 95.52
Cyazofamid suspensibility percent min. 70 94.87 92.55 93.85
Pyraclostrobin suspensibility percent min. 70 96.56 94.05 96.21
pH range (1% aq. Suspension) 5.5 to7.5 7.1 7.1 7.1
Wettability sec. max. 60 10 13 10
Wet Sieve(45 micron ) percent by mass min. 98.5 99.6 99.4 99.5
Bulk density (g/ml) 0.45 to 0.75 0.49 0.49 0.49
Moisture content percent by mass max. Max. 2.0% 1.6 1.2 1

Room temperature storage
Parameters Specification (In house) Study Duration
1 month 6 month 12 months 24 months
Description Off white colour granules Complies Complies Complies Complies
Dimethomorph content percent by mass 23.75 to 26.25 25.23 25.22 25.20 25.19
Cyazofamid content percent by mass 7.41 to 8.58 7.88 7.87 7.86 7.85
Pyraclostrobin content percent by mass 9.5 to 10.5 10.20 10.19 10.18 10.16
Dimethomorph suspensibility percent min. 70 95.81 95.72 95.51 94.57
Cyazofamid suspensibility percent min. 70 94.81 94.52 94.05 92.87
Pyraclostrobin suspensibility percent min. 70 96.41 95.95 95.42 94.15
pH range (1% aq. Suspension) 5.5 to7.5 7.1 7.1 7.1 7.1
Wettability sec. max. 60 10 11 12 12
Wet Sieve(45 micron ) percent by mass min. 98.5 99.6 99.5 99.5 99.4
Bulk density (g/ml) 0.45 to 0.75 0.49 0.49 0.49 0.49
Moisture content percent by mass max. Max. 2.0% 1.6 1.6 1.4 1.2

Example 6: Dimethomorph 25%+Cyazofamid 7.8%+Boscalid 6.25% WG
Chemical Composition Percent (% w/w)
Dimethomorph a.i. 25.0
Cyazofamid a.i. 7.8
Boscalid a.i. 6.25
Alkylated naphthalene sulfonate, sodium salt 6.0
Polyacrylate polymer sodium salt 2.0
Sodium Lauryl Sulfate 3.0
Silicone antifoam 1.0
Corn starch 8.0
China Clay QS
TOTAL 100.0

Procedure: Example 1
Storage stability study-Dimethomorph 25%+Cyazofamid 7.8%+Boscalid 6.25% WG
Parameters Specification (In house) Initial Stability (for 14 days)
At 54±2 0C At 0±2 0C
Description Off white colour granules Complies Complies Complies
Dimethomorph content percent by mass 23.75 to 26.25 25.22 25.14 25.20
Cyazofamid content percent by mass 7.41 to 8.58 7.90 7.82 7.86
Boscalid content percent by mass 5.93 to 6.87 6.35 6.26 6.33
Dimethomorph suspensibility percent min. 70 94.55 92.41 94.52
Cyazofamid suspensibility percent min. 70 94.91 92.05 93.47
Boscalid suspensibility percent min. 70 96.57 94.15 96.28
pH range (1% aq. Suspension) 5.5 to7.5 7.1 7.1 7.1
Wettability sec. max. 60 10 12 10
Wet Sieve(45 micron ) percent by mass min. 98.5 99.6 99.3 99.5
Bulk density (g/ml) 0.45 to 0.75 0.49 0.49 0.49
Moisture content percent by mass max. Max. 2.0% 1.6 1.2 1

Room temperature storage data
Parameters Specification (In house) Study Duration
1 month 6 month 12 months 24 months
Description Off white colour granules Complies Complies Complies Complies
Dimethomorph content percent by mass 23.75 to 26.25 25.21 25.20 25.18 25.17
Cyazofamid content percent by mass 7.41 to 8.58 7.88 7.86 7.85 7.84
Boscalid content percent by mass 5.93 to 6.87 6.33 6.32 6.31 6.30
Dimethomorph suspensibility percent min. 70 93.81 93.72 93.51 93.01
Cyazofamid suspensibility percent min. 70 94.77 94.52 94.05 92.54
Boscalid suspensibility percent min. 70 96.38 95.91 95.47 94.85
pH range (1% aq. Suspension) 5.5 to7.5 7.1 7.1 7.1 7.1
Wettability sec. max. 60 10 11 12 12
Wet Sieve(45 micron ) percent by mass min. 98.5 99.6 99.5 99.5 99.4
Bulk density (g/ml) 0.45 to 0.75 0.49 0.49 0.49 0.49
Moisture content percent by mass max. Max. 2.0% 1.6 1.4 1.2 1.2

Example 7: Recipe-Dimethomorph 16.00 % + Metalaxyl-M 3.20 % +Azoxystrobin 6.40 % SC
Chemical Composition Percent (% w/w)
Dimethomorph a.i. 16.0
Metalaxyl-M a.i. 3.2
Azoxystrobin a.i. 6.4
Ethoxylated Fatty Alcohol 2.0
Acrylic graft copolymer 3.0
Alkylated naphtalene sulfonate, sodium salt 0.5
Silicone antifoam 0.5
1,2-benzisothiazolin-3-one 0.20
Mono Ethylene Glycol 5.0
Polysaccharides 0.15
Trisiloxane ethoxylate 2.0
Water QS
TOTAL 100.0

Procedure:
Step 1 Gum Solution should be made 12-18 hour prior to use. Take required quantity of water, biocide, and defoamer and homogenize, then slowly add gum powder to it and stir till complete dissolution.
Step 2 Charge required quantity of DM water need to be taken in designated vessel for Suspension concentrate production.
Step 3 Add required quantity of Wetting agent, antifreeze, dispersing agent & suspending agents and homogenize the contents for 45 – 60 minutes using high shear homogenizer.
Step 4 Then add technical and other remaining adjuvants excluding ‘thickener’ are added to it and homogenized to get uniform slurry ready for grinding.
Step 5 Before grinding half the quantity of antifoam was added and then material was subjected to grinding in Dyno mill till desired particle size is achieved.
Step 6 Half quantity of the antifoam was added after grinding process completes and before sampling for in process analysis.
Step 7 Finally add gum solution to this formulation and send to QC dept. for quality check.

Storage stability study-Dimethomorph 16.00 % + Metalaxyl-M 3.20 % +Azoxystrobin 6.40 % SC
Parameters Specification (In house) Initial Stability
(for 14 days)
At
54±2 0C At
0±2 0C
Description White to Off White colour flowable liquid Complies Complies Complies
Dimethomorph content percent by mass 15.20 to 16.80 16.20 16.08 16.19
Metalaxyl-M content percent by mass 3.04 to 3.52 3.27 3.24 3.27
Azoxystrobin content percent by mass 6.08 to 7.04 6.46 6.41 6.45
Dimethomorph suspensibility percent mini. 80 98.95 97.05 98.69
Metalaxyl-M suspensibility percent mini. 80 97.01 95.50 96.55
Azoxystrobin suspensibility percent mini. 80 96.95 95.06 95.75
pH range (1% aq. Suspension) 5.5 to7.5 7.2 7.1 7.2
Pourability 95 % min 98.50 97.70 97.60
Specific gravity 1.05 – 1.15 1.07 1.08 1.07
Viscosity at spindle no.62, 20 rpm 350 -800 cps 510 530 535
Particle size (micron) D50 <3, D90 <10 2.2, 7.9 2.4, 8.5 2.7, 8.5
Persistent foam ml (after 1 minute) max. 60 3 5 5

Room temperature storage data
Parameters Specification (In house) Study Duration
1 month 6 month 12 months 24 months
Description White to Off White colour flowable liquid Complies Complies Complies Complies
Dimethomorph content percent by mass 15.20 to 16.80 16.19 16.18 16.17 16.12
Metalaxyl-M content percent by mass 3.04 to 3.52 3.27 3.27 3.25 3.25
Azoxystrobin content percent by mass 6.08 to 7.04 6.46 6.45 6.44 6.43
Dimethomorph suspensibility percent mini. 80 97.91 97.78 97.71 97.55
Metalaxyl-M suspensibility percent mini. 80 96.95 96.50 96.25 95.50
Azoxystrobin suspensibility percent mini. 80 96.85 96.71 96.51 95.81
pH range (1% aq. Suspension) 5.5 to7.5 7.2 7.2 7.2 7.1
Pourability 95 % min 98.50 98.40 97.20 96.80
Specific gravity 1.05 – 1.15 1.07 1.07 1.08 1.08
Viscosity at spindle no.62, 20 rpm 350 -800 cps 510 515 518 526
Particle size (micron) D50 <3, D90 <10 2.2, 8 2.2, 8.1 2.3, 8.2 2.4, 8.3
Persistent foam ml (after 1 minute) max. 60 3 3 5 5

Example 8: Bio efficacy trial
Biological Examples:
The synergistic fungicidal action of the inventive mixtures can be demonstrated by the experiments below. A synergistic effect exists wherever the action of a combination (ready-mix) of active ingredient is greater than the sum of the action of each of the components alone. Therefore a synergistically effective amount or an effective amount of a synergistic composition or combination is an amount that exhibits greater pesticide activity than the sum of the pesticide activities of the individual components. In the field of agriculture, it is often understood that the term “synergy” is as defined by Colby S.R. in an article entitled “ Calculation of the synergistic and antagonistic responses of herbicide combinations” published in the journal Weeds, 1967, 15, p.20-22, incorporated herein by reference in its entirety. The action expected for a given combination of two active components can be calculated as follows:
Colby’s Formula:

To study the synergistic effect of below mentioned combinations were studied in laboratory as well as field trials were carried out on different crops.
Table 1 : Lists of combinations evaluated for synergism
Active Ingredient I Active Ingredient II Active Ingredient III

Dimethomorph Metalaxyl-M Mancozeb
Dimethomorph Metalaxyl-M Azoxystrobin
Dimethomorph Metalaxyl-M Pyraclostrobin
Dimethomorph Metalaxyl-M Boscalid
Dimethomorph Cyazofamid Mancozeb
Dimethomorph Cyazofamid Azoxystrobin
Dimethomorph Cyazofamid Pyraclostrobin
Dimethomorph Cyazofamid Boscalid

Trial 1
Laboratory screening of mixtures given in Table 1, were carried out to evaluate their efficacy against various fungi causing fungal diseases in crop plants. The synergistic effect on growth of the pathogen in vitro were studied by poison food technique. Different concentration of active ingredient were prepared by dissolving the requisite quantity of each active ingredient in warm potato dextrose agar medium before autoclaving. After autoclaving the medium was then dispensed uniformly into 90 mm diameter petriplate and inoculated at the 2 mm mycelial discs of pathogen taken from 7 to 10 days old culture. Pathogen inoculated in unamended medium served as control. Each treatment was maintained in four replications. The inoculated plates were incubated at 30+/- 2 C for 3 days and the diameter of the fungal colony was measured by measuring the two opposite circumference of the colony growth at 3 days interval for 15 days. The growth of fungus was monitored by measuring the radial growth (in mm) every 72 h till the fungus covers the plate completely in control plates. The percent inhibition (PI) of the fungus over the control was calculated using the following formula :
PI = A-B X 100/A
Where A= Colony diameter of fungus in control plates (mm)
B= Colony diameter of fungus in treated plates (mm)
The synergistic fungicidal action of the inventive mixtures calculated as per the Colby’s formula given above by using percent inhibition (PI) data.
Table 2 : Effect on mycelial growth of Phytophthora infestans
Treatments Concentrations (ppm) Phytophthora infestans
Mycelial growth (mm) % Inhibition over control
Dimethomorph+Metalaxyl-M+Mancozeb 80+12.5+225 0.0 100.00
Dimethomorph+Cyazofamid+Mancozeb 80+12.5+225 0.0 100.00
Dimethomorph+Metalaxyl-M 80+12.5 28.4 69.98
Dimethomorph+Cyazofamid 80+12.5 29.2 69.13
Dimethomorph+Mancozeb 80+225 37.6 60.25
Metalaxyl-M+Mancozeb 12.5+225 36.4 61.52
Cyazofamid+Mancozeb 12.5+225 38.8 58.99
Dimethomorph 80 40.2 57.51
Metalaxyl-M 12.5 57.2 39.53
Cyazofamid 12.5 56.4 40.38
Mancozeb 225 62.4 34.04
Untreated Check (UTC) 0 94.6 0.00

Table 3: Synergistic activity on mycelial growth of Phytophthora infestans
Treatments Concentrations (ppm) % Growth Inhibition of Phytophthora infestans
Obs. Value Exp. Value Ratio O/E
Dimethomorph+Metalaxyl-M+Mancozeb 80+12.5+225 100.00 84.68 1.18
Dimethomorph+Cyazofamid+Mancozeb 80+12.5+225 100.00 83.05 1.20
Dimethomorph+Metalaxyl-M 80+12.5 69.98 74.31 0.94
Dimethomorph+Cyazofamid 80+12.5 69.13 74.66 0.93
Dimethomorph+Mancozeb 80+225 60.25 71.97 0.84
Metalaxyl-M+Mancozeb 12.5+225 61.52 63.95 0.96
Cyazofamid+Mancozeb 12.5+225 58.99 60.12 0.98
Dimethomorph 80 57.51
Metalaxyl-M 12.5 39.53
Cyazofamid 12.5 40.38
Mancozeb 225 34.04
Untreated Check (UTC) 0 0.00

In vitro studies indicated the suppressive effect of Dimethomorph+Metalaxyl-M+Mancozeb and Dimethomorph+Cyazofamid+Mancozeb on the radial growth of the mycelium of Phytophthora infestans (Table 2). It can be seen that the synergism was observed between Dimethomorph + Metalaxyl-M + Mancozeb and Dimethomorph +Cyazofamid + Mancozeb in terms of excellent suppressive effect on radial growth of Phytophthora infestans (Table 3).
Trial 2 :
As per the methodology given in example 1, laboratory screening of mixtures given in table 1, were evaluated for their efficacy against early blight fungus, Alternaria solani by poison food technique. The results were presented in table 4 and table 5.
Table 4: Effect on mycelial growth of Alternaria solani
Treatments Concentrations (ppm) Alternaria solani
Mycelial growth (mm) % Inhibition over control
Dimethomorph+Metalaxyl-M+Azoxystrobin 80+12.5+40 0.0 100.00
Dimethomorph+Metalaxyl-M+Pyraclostrobin 80+12.5+40 0.0 100.00
Dimethomorph+Metalaxyl-M+Boscalid 80+12.5+25 0.0 100.00
Dimethomorph+Cyazofamid+Azoxystrobin 80+12.5+40 0.0 100.00
Dimethomorph+Cyazofamid+Pyraclostrobin 80+12.5+40 0.0 100.00
Dimethomorph+Cyazofamid+Boscalid 80+12.5+25 0.0 100.00
Dimethomorph+Metalaxyl-M 80+12.5 39.8 55.91
Dimethomorph+Cyazofamid 80+12.5 38.2 57.69
Dimethomorph+Azoxystrobin 80+40 30.4 66.33
Dimethomorph+Pyraclostrobin 80+40 28.4 68.54
Dimethomorph+Boscalid 80+25 35.6 60.57
Metalaxyl-M+Azoxystrobin 12.5+40 33.8 62.56
Metalaxyl-M+Pyraclostrobin 12.5+40 31.2 65.44
Metalaxyl-M+Boscalid 12.5+25 35.2 61.01
Cyazofamid+Azoxystrobin 12.5+40 38.6 57.24
Cyazofamid+Pyraclostrobin 12.5+40 34.2 62.12
Cyazofamid+Boscalid 12.5+25 40.4 55.25
Dimethomorph 80 56.4 37.53
Metalaxyl-M 12.5 61.8 31.55
Cyazofamid 12.5 60.6 32.88
Azoxystrobin 40 46.4 48.60
Pyraclostrobin 40 44.2 51.04
Boscalid 25 47.8 47.05
Untreated Check (UTC) 0 90.3 0.00

Table 5: Synergistic activity on mycelial growth of Alternaria solani
Treatments Concentrations (ppm) % Growth Inhibition of Phytophthora infestans
Obs. Value Exp. Value Ratio O/E
Dimethomorph+Metalaxyl-M+Azoxystrobin 80+12.5+40 100.00 78.02 1.28
Dimethomorph+Metalaxyl-M+Pyraclostrobin 80+12.5+40 100.00 79.06 1.26
Dimethomorph+Metalaxyl-M+Boscalid 80+12.5+25 100.00 77.36 1.29
Dimethomorph+Cyazofamid+Azoxystrobin 80+12.5+40 100.00 78.45 1.27
Dimethomorph+Cyazofamid+Pyraclostrobin 80+12.5+40 100.00 79.47 1.26
Dimethomorph+Cyazofamid+Boscalid 80+12.5+25 100.00 77.80 1.29
Dimethomorph+Metalaxyl-M 80+12.5 55.91 57.24 0.98
Dimethomorph+Cyazofamid 80+12.5 57.69 58.07 0.99
Dimethomorph+Azoxystrobin 80+40 66.33 67.89 0.98
Dimethomorph+Pyraclostrobin 80+40 68.54 69.41 0.99
Dimethomorph+Boscalid 80+25 60.57 66.92 0.91
Metalaxyl-M+Azoxystrobin 12.5+40 62.56 64.82 0.97
Metalaxyl-M+Pyraclostrobin 12.5+40 65.44 66.49 0.98
Metalaxyl-M+Boscalid 12.5+25 61.01 63.76 0.96
Cyazofamid+Azoxystrobin 12.5+40 57.24 65.50 0.87
Cyazofamid+Pyraclostrobin 12.5+40 62.12 67.14 0.93
Cyazofamid+Boscalid 12.5+25 55.25 64.46 0.86
Dimethomorph 80 37.53
Metalaxyl-M 12.5 31.55
Cyazofamid 12.5 32.88
Azoxystrobin 40 48.60
Pyraclostrobin 40 51.04
Boscalid 25 47.05
Untreated Check (UTC) 0 0.00

The mixture of Dimethomorph+Metalaxyl-M+Azoxystrobin, Dimethomorph+Metalaxyl-M+Pyraclostrobin, Dimethomorph+Metalaxyl-M+Boscalid, Dimethomorph+ Cyazofamid+ Azoxystrobin, Dimethomorph+Cyazofamid+Pyraclostrobin, Dimethomorph+ Cyazofamid+ Boscalid shows synergism in terms of their efficacy against Alternaria solani by suppressing mycelia growth.
Trial 3: Disease control in Potato
The synergistic mixtures of were also evaluated in potato (Solanum tuberosum) crop.
Details of Experiment:
a) Experiment design : Randomized Block Design
b) Replication : Three
c) Treatments : Twenty including untreated check.
d) Plot size : 20 sq. m.
e) Spacing : Row to Row 60 cm, Plant to platn 25 cm
f) Test Crop & Variety : Potato, Kufri Badshah
g) Time of application : Two spray at 10 days interval
h) Spray Volume : 500 l/h
i) Method of application : Foliar spray with knap sack sprayer
The Potato crop was raised in the field as per the standard agronomic practices. The spraying was done as preventive spray and second spray at 10 days after first spray. The observations on disease severity and incidence of late blight were recorded at 7 days after second application.

1) Late blight disease :
Record the number of healthy and infected leaves from 100 leaves per plot and calculate the percent disease incidence.
The disease severity recorded on 100 leaves by following 0-9 scale described as below;
Grade Percent branches & foliage infected per plant
0 No infection to branches and leaves
1 1-10% leaves infected
3 11-25% leaves infected
5 26-50% leaves infected
7 51-75% leaves infected
9 >75% leaves infected.

% Disease Incidence on leaves = No. of leaves infected X 100.
No. of leaves observed

Late Blight PDI (Severity %) =
Sum of numerical disease rating X 100
Total no. of samples X Maximum of disease rating scale

Table 6: Bioefficacy of synergistic mixtures against Late blight of Potato, Phytophthora infestans
Composition, g/h g.a.i./h Late Blight, Phytophthora infestans
Incidence (%) PDI / Severity (%)
Dimethomorph 16%+Metalaxyl-M 2.5%+Mancozeb 45%,WG, 1000 g/h 160+25+450 1.20 0.12
Dimethomorph 16%+Metalaxyl-M 2.5%+Mancozeb 45%,WP, 1000 g/h 160+25+450 1.26 0.18
Dimethomorph 20%+Metalaxyl-M 4%+Azoxystrobin 8%, WG, 800 g/h 160+32+64 1.84 0.48
Dimethomorph 20%+Metalaxyl-M 4%+Pyraclostrobin 8%, WG, 800 g/h 160+32+64 2.36 0.36
Dimethomorph 25%+Metalaxyl-M 5%+Boscalid 6.25%,WG, 800 g/h 200+40+50 4.18 1.42
Dimethomorph 16%+Cyazofamid 5%+Mancozeb 50%, WG, 1000 g/h 160+50+500 1.92 0.26
Dimethomorph 20%+Cyazofamid 6.25%+Azoxystrobin 8%,WG, 800 g/h 160+50+64 2.10 0.34
Dimethomorph 25%+Cyazofamid 7.8%+Pyraclostrobin 10%,WG, 640 g/h 160+50+64 1.32 0.62
Dimethomorph 25%+Cyazofamid 7.8%+Boscalid 6.25%,WG, 640 g/h 160+50+40 2.62 1.12
Dimethomorph 16%+Metalaxyl-M 2.5%,WG, 1000 g/h 160+25 10.84 5.84
Metalaxyl-M 2.5%+Mancozeb 45%,WG, 1000 g/h 25+450 14.28 9.28
Dimethomorph 20%+Azoxystrobin 8%, WG, 800 g/h 160+64 20.84 10.62
Dimethomorph 20%+Pyraclostrobin 8%, WG, 800 g/h 160+64 18.26 9.74
Dimethomorph 25%+Boscalid 6.25%,WG, 800 g/h 200+50 24.64 14.62
Metalaxyl-M 2.5%+Mancozeb 45%,WG, 1000 g/h 25+450 28.10 22.16
Metalaxyl-M 4%+Azoxystrobin 8%, WG, 800 g/h 32+64 26.36 18.80
Metalaxyl-M 4%+Pyraclostrobin 8%, WG, 800 g/h 32+64 28.84 22.74
Metalaxyl-M 5%+Boscalid 6.25%,WG, 800 g/h 40+50 44.68 30.72
Dimethomorph 16%+Cyazofamid 5%.WG, 1000 g/h 160+50 15.82 7.72
Cyazofamid 5%+Mancozeb 50%, WG, 1000 g/h 50+500 21.34 10.82
Cyazofamid 6.25%+Azoxystrobin 8%,WG, 800 g/h 50+64 27.66 18.72
Cyazofamid 7.8%+Pyraclostrobin 10%,WG, 640 g/h 50+64 30.64 23.16
Cyazofamid 7.8%+Boscalid 6.25%,WG, 640 g/h 50+40 33.85 28.72
Dimethomorph 25% WG, 800 g/h 200 62.48 40.72
Metalaxyl-M 5%, WG, 800 g/h 40 66.82 45.62
Cyazofamid 5%, WG, 1000 g/h 50 68.28 42.63
Mancozeb 50%, WG,1000 g/h 500 78.84 62.34
Azoxystrobin 8%, WG, 800 g/h 64 72.38 50.82
Pyraclostrobin 8%, WG, 800 g/h 64 80.28 64.58
Boscalid 6.25%,WG,640 g/h 40 84.26 75.82
Untreated Check (UTC) 0 88.82 89.84
g/h Gram per hectare, g.a.i/h Gram active ingredient per hectare, PDI Percent Disease Index, WG Water Dispersible Granules

The field efficacy observations of inventive combinations shows synergism and provides excellent control of potato late blight caused by Phytophthora infestans under field conditions compared to the prior art samples.
,CLAIMS:CLAIMS

[CLAIM 1]. A synergistic fungicide composition comprising Dimethomorph and component (A) first fungicide selected from Metalaxyl-M, Cymoxanil, Cyazofamid, Fluopicolide and component (B) second fungicide selected from Captan, Chlorothalonil, Fluazinam, Folpet, Mancozeb, Metiram, Propineb, Ziram, Zoxamide, Boscalid, Azoxystrobin, Pyraclostrobin, Trifloxystrobin and one or more inactive excipients.

[CLAIM 2]. The synergistic fungicide composition as claimed in claim 1 wherein Dimethomorph is in the range of 0.1 to 25% and component (A) first fungicide is in the range of 0.1 to 25% and component (B) second fungicide is in the range of 0.1 to 50%.

[CLAIM 3]. The synergistic fungicide composition as claimed in claim 1 or 2, wherein inactive excipients are selected from the group consisting of dispersing agent, anti-freezing agent, anti-foam agent, wetting agent, suspension aid, anti-microbial agent, thickener, quick coating agent or sticking agents and buffering agent.

[CLAIM 4]. The synergistic fungicide composition as claimed in claims 1-3, wherein the composition are selected from composition comprising of water-dispersible granules (WDG), suspension concentrate (SC) and wettable powder (WP).

[CLAIM 5]. The synergistic fungicidal composition according to claim 1 or 2, is in the form of suspension concentrate (SC) comprising
a) 0.1 to 25% Dimethomorph;
b) 1% to 25% component (A) first fungicide selected from Metalaxyl-M, Cymoxanil, Cyazofamid, Fluopicolide
c) 1% to 50% component (B) second fungicide selected from Captan, Chlorothalonil, Fluazinam, Folpet, Mancozeb, Metiram, Propineb, Ziram, Zoxamide, Boscalid, Azoxystrobin, Pyraclostrobin, Trifloxystrobin and
d) 0.2 to 20% dispersing agent selected from guar gum; locust bean gum; carrageenam; xanthan gum; alginates; methyl cellulose; sodium carboxymethyl cellulose (SCMC); hydroxyethyl cellulose (HEC), modified starches, polyacrylates salts, polyvinyl alcohol and polyethylene oxide or mixtures thereof.

[CLAIM 6]. The synergistic fungicidal composition according to claim 1 or 2, is in the form of Water dispersible granules (WG) or wettable powder (WP) comprising;
a) 0.1 to 25% Dimethomorph;
b) 1% to 25% component (A) first fungicide selected from Metalaxyl-M, Cymoxanil, Cyazofamid, Fluopicolide
c) 1% to 50% component (B) second fungicide selected from Captan, Chlorothalonil, Fluazinam, Folpet, Mancozeb, Metiram, Propineb, Ziram, Zoxamide, Boscalid, Azoxystrobin, Pyraclostrobin, Trifloxystrobin and
d) 0.2 to 20% thickener selected from guar gum; locust bean gum; carrageenam; xanthan gum; alginates; methyl cellulose; sodium carboxymethyl cellulose (SCMC); hydroxyethyl cellulose (HEC), modified starches, polyacrylates salts, polyvinyl alcohol and polyethylene oxide or mixtures thereof

[CLAIM 7]. The synergistic fungicidal composition according to any preceding claim, wherein dispersing agent is polyacrylates salt.

[CLAIM 8]. The synergistic fungicidal composition as claimed in in any preceding claims, wherein the composition effective for management of mixed infection offungal diseases in following cropsCotton (Gossypium spp.), Jute (Corchorusoliotorus), Paddy (Oryza sativa), Wheat (Triticumaestavum), Barley (Hordeumvulgare), Maize (Zea mays), Sorghum (Sorghum bicolor), Ragi (Eleusinecoracana), Pearl millet (Pennisetumglaucum), Sugarcane (Saccharumofficinarum) , Sugarbeet (Beta vulgaris), Soybean (Glycin max), Peanut (Arachishypogaea), Sunflower (Helianthus annuus) , Mustard (Brassica juncea), Rape seed (Brassica napus), Linseed (Linumusitatissimum), Sesame (Sesamumindicum), Castor (Ricinuscommunis), Green gram (Vigna radiate), Black gram (Vignamungo), Chickpea (Ciceraritinum), Cowpea (Vignaunguiculata), Redgram (Cajanuscajan), Frenchbean (Phaseolus vulgaris), Indian bean (Lablab purpureus), Horse gram (Macrotylomauniflorum), Field pea (Pisumsativum), Cluster bean (Cyamopsistetragonoloba), Lentils (Lens culinaris), Brinjal (Solanummelongena), Cabbage (Brassica oleracea var. capitata), Cauliflower (Brassica oleracea var. botrytis), Okra (Abelmoschusesculentus) , Onion (Allium cepa L.), Tomato (Solanumlycopersicun) , Potato (Solanumtuberosum) , Sweet potato (Ipomoea batatas), Chilly (Capsicum annum), Garlic (Allium sativum), Cucumber (Cucumissativus), Muskmelons (Cucumismelo), Watermelon (Citrulluslanatus), Bottle gourd (Lagenariasiceraria), Bitter gourd (Momordicacharantia), Radish (Raphanussativus), Carrot (Dacuscarota subsp. sativus), Turnip (Brassica rapasubsprapa), Apple (Melusdomestica), Banana (Musa spp.), Citrus groups (Citrus spp.), Grape (Vitisvinifera), Guava (Psidiumguajava), Litchi (Litchi chinensis), Mango (Mangiferaindica), Papaya (Carica papaya), Pineapple (Ananascomosus), Pomegranate (Punicagranatum) , Sapota (Manilkarazapota), Tea (Camellia sinensis), Coffea (Coffea Arabica), Turmeric (Curcuma longa), Ginger (Zingiberofficinale), Cumin (Cuminumcyminum), Fenugreek (Trigonellafoenum-graecum), Fennel (Foeniculumvulgare), Coriander (Coriandrumsativum), Ajwain (Trachyspermumammi), Psyllium (Plantago ovate), Black Pepper (Piper nigrum), Stevia (Stevia rebaudiana), Safedmusli (Chlorophytumtuberosum), Drum stick (Moringaoleifera), Coconut (Coco nucifera), Mentha ( Mentha spp.), Rose (Rosa spp.), Jasmine (Jasminum spp.), Marigold ( Tagetes spp.), Common daisy (Bellisperennis), Dahlia (Dahlia hortnesis), Gerbera (Gerbera jamesonii), Carnation (Dianthus caryophyllus)

[CLAIM 9]. The synergistic fungicidal composition as claimed in in any preceding claims, wherein the composition effective for disease selected from Pythium blight (high temperature) by Pythium aphanidermatum, Pythium blight (low temperature) by Pythium graminicola, Pythium ultimum, Pythium damping off diseases in nurseries of tobacco, tomato, chillies, egg-plant; Phytophthora species such as, Phytophthora infestans causing late blight in potato and tomato, Gummosis and Root rot in citrus by Phytophthora spp., seed rot, seedling blight, fruit rot in chilli and cucurbits by Phytophthora capsici, downy mildew of cucurbits by Pseudoperonospora cubensis, downy mildew of millets by Sclerospora graminicola, downy mildew of maize by Peronosclerospora sroghi, white rust of crucifers by Albugo candida, downy mildew of grape by Plasmopara viticola, leaf spot, leaf blight caused by Alternaria solani; Cercospora species, Colletotrichum species, Septoria species in fruits and vegetables.