The present invention relates to "Gelatine filter manufacturing and making of the same" and more particularly to Manufacturing of the same.
[0002] BACKGROUND OF THE.INVENTION
[0003] The meaning of "Gelatine filter" is by Gelatine. Gelatin Filter is white, sterile, 37mm,
3|im: Gelatin Filters 37 mm | Sterile, polyethylene bags each 38,5 cm2 Diameter: 37 mm and the manufacturing method of t,he gelatin is implemented by virtue of the following technical schemes: pre-soaking a leather material for 2 days by using lime water containing 1% of calcium oxide; cutting the leather material into small blocks, soaking into the 2-4% lime water, and periodically changing the lime water; transferring the leather material into a neutralization tank, and continuously stirring; removing calcium salt which is formed by combining collagen and calcium oxide in the leather material by using acid, and then removing the excess acid and generated salts by using clean water; firstly feeding a certain amount of water into a gelatin cooking pot, heating to the minimum gelatin cooking temperature, then pouring the water-washed leather material into the gelatin cooking pot, then feeding water again to ensure that the leather material is just immersed, and slowly heating to the gelatin cooking temperature; heating a gelatin solution in a concentration pan to ensure that moisture is evaporated, discharging the concentrated gelatin solution, and adding a certain amount of preservatives; and injecting the gelatin solution added with the preservatives into a gelatin freezing disc, and freezing into blocks in brine ice bath.
OBJECTS OF THE INVENTION
[0004] The main objective of the invention of gel-filtration chromatography is that separation can be performed under conditions specifically designed to maintain the stability and activity of the molecule of interest without compromising resolution
[0005] The objective of gelatine filter invention is for High moisture content
a. Maintain microbe viability for short sampling periods
b. Completely water soluble
c. Dissolve easily when placed on agar
d. Prerequisite for virus sampling
e. Pre-sterilised by gamma irradiation
f. Ideal for monitoring in pharmaceutical plants
g. Can be used to monitor in areas where disinfectants or antibiotics are present
[0006] The main objective of this invention is to utilise in the following sectors :

a. Equipment of airtanalyzers for Indoor Air Quality
b. Equipment for air Analysers of Outdoor environmental measurements
c. Clean Room Monitoring air filter
d. Veterinary clinics
e. Hospitals
f. Pharmaceutical manufacturing
g. And other air quality monitoring areas
SUMMARY OF THE INVENTION
[0007] Gelatin is a protein substance derived from collagen, a natural protein present in the tendons, ligaments, and tissues of mammals. It is produced by boiling the connective tissues, bones and skins of animals, usually cows and pigs. Gelatin's ability to form strong, transparent gels and flexible films that are easily digested, soluble in hot water, and capable of forming a positive binding action have made it a valuable commodity in food processing, pharmaceuticals, photography, and paper production.
[0008] As a foodstuff, gelatin is the basis for jellied desserts; used in the preservation of fruit and meat, and to make powdered milk, merinque, taffy, marshmallow, and fondant. It is also used to clarify beer and wine. Gelatin's industrial applications include medicine capsules, photographic plate coatings, and dying and tanning supplies.
[0009] The Manufacturing Process
[0010] Inspection and cutting
[0011] 1 When the animal parts arrive at the food processing plant, they are inspected for quality. Rotted parts are discarded. Then, the bones, tissues, and skins are loaded into chopping machines that cut the parts into small pieces of about Sin (12.7cm) in diameter.
[0012] Degreasing and roasting
[0013] 2 The animal parts are passed under high-pressure water sprays to wash away debris. They are then degreased by soaking them in hot water to reduce the fat content to about 2%. A conveyer belt moves the degreased bones and skins to an industrial dryer where they are roasted for approximately 30 minutes at about 200° F (100° C).
[0014] Acid and akaline treatment
[0015] 3 The animal parts are soaked in vats of lime or some other type of acid or akali for approximately five days. This process removes most of the minerals and bacteria and facilitates the release of collagen. The acid wash is typically a 4% hydrochloric acid with a pH of less than 1.5. The alkaline wash is a potassium or sodium carbonate with a pH above 7.
[0016] Boiling
[0017] 4 The pieces of bone, tissue, and skin are loaded into large aluminum extractors and boiled in distilled water. A tube running from the extractor allows workers to draw off the

liquid that now contains gelatin. The liquid is sterilized by flash-heating it to about 375° F (140° C) for approximately four seconds.
[0018] Evaporating and grinding
[0019] 5 From the extractor, the liquid is piped through filters to separate out bits of bone, tissue or skin that are still attached. From the filters, the liquid is piped into evaporators, machines that separate the liquid from the solid gelatin. The liquid is piped out and discarded. The gelatin is passed through machines that press it into sheets. Depending on its final application, the gelatin sheets are passed through a grinder that reduces them to a fine powder. The chemical structure of gelatin is what makes gelatin water soluble; form digestible gels and films that are strong, flexible, and transparent; and form a positive binding action that is useful in food processing, pharmaceuticals, photography, and paper production.
[0020] Flavoring and coloring
[0021] 6 If the gelatin is to be used by the food industry, sweeteners, flavorings, and colorings may be added at this point. PreVset amounts of these additives are thoroughly mixed into the powdered gelatin.

CMj
/CH* CBj
f 1 CHOH
/\ CKj CH,
co= 1 1 ON, N—CH NH
/\/ \/= KH CO CO CH =
1 1 1
CHj NN N—CH
/\/Y / = CO==NH CO CH=CO CO t
1 R 1 J)
[0022] Packaging: Sterile, polyethylene bags each with 5 filters Filtration area: 38,5 cm2
Gelatine filters in conjunction with the MD8 air samplers (gelatine filter method) are used for
collection of airborne microbes and viruses. Gelatine filter disposables are individually
packaged, presterilised and ready-to-connect units, each consisting of a gelatine membrane
filter and a holder. Gelatine membrane filters are still available as filter discs, suitable for the
filter holder 17655 (80 mm diameter) supplied with the MD8 airscan® air samplers as well as
in smaller diameters.
[0023] The packaging process is automated, with preset amounts of gelatin poured into overhead funnels through which the gelatin flows down into bags made of either polypropylene or multi-ply paper. The bags are then vacuumed sealed.
[0024] Flow rate for air at 1 bar: P = 0.05 bar: ~2,7 l/min Sterilisation: Supplied presterilized by gamma irradiation Temperature: max. 60°C Ambient conditions: Room temperature, max.

thickness approx. 250u.m Retention rates Bac. subtilis niger spores 99.9995% at 0.25m/s inlet velocity Coli-Phages: phage Tl, 99.9% at 0.3m/s inlet velocity and 50% rel. air humidity Phage T3, 99.94% at 0.3 m/s inlet velocity and 80% rel. air humidity.
[0025] Gelatine filters in conjunction with the MD8 air samplers (gelatine filter method) are used for collection of airborne microbes and viruses - individually, sterile packaged membranes guarantee reliable and accurate results.
The Making process Gelatin
[0026] Unflavored gelatin is produced from animal collagen and can be mixed ahead of time
to set almost any liquid, including drinks, jams, jellies and fillings. By using powdered or sheet
gelatin from the grocery store, you can customize the firmness of your dessert. This article
will show you how to prepare gelatin using both the powdered and sheet forms. It will also
give you some ideas on how you can further customize your gelatin.
[0027] Types of Gel Filtration Chromatography
Group Separations
• The components of a sample are separated into two major groups according to the size range.
• A group separation can be used to remove high or low molecular weight contaminants (such as phenol red from culture fluids) or to desalt and exchange buffers.
High-resolution fractionation of biomolecules
• The components of a sample are separated according to differences in their molecular size.
• High-resolution fractionation can be used to isolate one or more components, to separate monomers from aggregates, to determine the molecular weight or to perform a molecular weight distribution analysis.
[0028] Gelatin powder is gelatin that has been dried and broken up into individual grains, which has the advantage if dispersing more easily throughout a dish. Gelatin sheets are made from gelatin that is dried in a flat sheet. Sheets result in a clearer, more transparent final product than powder.'As far as we could tell from our research, gelatin sheets and gelatin leaves are just different names for the same product.
DETAILED DESCRIPTION OF THE INVENTION
[0029] Ingredients Powdered Gelatin
Yi cup (112.50 milliliters) cold water
1 tablespoon powdered gelatin (1 packet)
1 Vi cups (337.5 millilitres) hot water
Gelatin Sheets
4 gelatin sheets
2 cup (225 milliliters) cold water

■ •h
The Making Process of Gelatine Filter
[0030] Mixing Powdered Gelatin; Image titled Make Gelatin
[0031] Step 1; powdered gelatin -1 tablespoon of gelatine
[0032]
[0033] Step 2; Pour % cup (112.50 milliliters) of cold water into a large bowl
[0034] Step 3; Pour the gelatin over the water, sprinkle the gelatin as evenly, After a few
minutes; the gelatin will start to expand.
[0035] Step 4; 1 Vi cups of hot water into the gelatin.
[0036] Step 5; Stir until the gelatin dissolves, use a spoon;
[0037] Step 6 Pour the gelatin into some molds, for shaped molds, lightly grease the inside of
each mold with a flavorless oil;
[0038] Step 7 Set in the refrigerator for 4 hours
[0039] Method; 2; Using Gelat'yi Sheets;
[0040] Step 1; Place the sheets into large; flat dish.the sheets are separated; and sitting right
next to each other, pouring water over them.
[0041] Step 2; Pour enough cold water to cover the sheets, about 1 cup of water.
[0042] Step 3; Wait until the gelatin sheets bloom, expand slightly and become wrinkly, will
take about five to six minutes.
[0043] Step 3; Prepare 2 cups of warm water to a simmer in a pot, adding the gelatin sheets
to it once they bloom.;
[0044] Step 4; Lift the sheets out of the water and remove any excess moisture.
[0045] Step 5; Add the gelatin sheets to the hot water and stir until the dissolve.
[0046] Step 6; Pour the gelatin into a mold.
[0047] Step 7; Leave the gelatin in the refrigerator until it sets. It will take about 4 hours for it
to become firm.;
Advantage of the invention
[0048] The main advantage o{ Biomolecules are purified using different techniques that separate them according to the differences in their specific properties such as size, hydrophobicity, biorecognition, charge, etc.
[0049] The advantage of Gel filtration is a technique in which the separation of components is based on the difference in molecular weight or size.
[0050] It is the simplest and mildest of all the chromatography techniques and separates molecules on the basis of differences in size
[0051] The stationary phase used is a porous polymer matrix whose pores are completely filled with the solvent to be used as the mobile phase.
[0052] The molecules in the sample are pumped through specialized columns containing such microporous packing material (gel).
[0053] The basis of the separation is that molecules above a certain size are totally excluded from the pores, while smaller molecules access the interior of the pores partly or wholly. [0054] The flow of the mobile phase hence will cause larger molecules to pass through the column unhindered, without penetrating the gel matrix, whereas smaller molecules will be retarded according to their penetration of the gel.
., t.

I Claim:

1. A manufacturing method of Gelatin filter is made from Gelatin sheets are made from gelatin that is dried in a flat sheet which is Sheets result in a clearer, more transparent final product than powder, the method comprising the steps of;
a. Place the sheets into large; flat dish.the sheets are separated; and sitting right
next to each other;
b. Pour enough cold water to cover the sheets, about 1 cup of water;
c. Wait until the gelatin sheets bloom, expand slightly and become wrinkly; will
take about five to six minutes;
d. Prepare 2 cups of warm water to a simmer in a pot; adding the gelatin sheets to
it once they bloom;
e. Lift the sheets out of the water and remove any excess moisture;
f. Add the gelatin sheets to the hot water andstir until the dissolve;
g. Pour the gelatin into a mold;
h. Leave the gelatin in the refrigerator until it sets; take about 4 hours for it to become firm;
2. The method as claimed in claiml, further comprises processing of Biomolecules are purified using different techniques that separate them according to the differences in their specific properties such as size, hydrophobicity, biorecognition, charge, etc.
3. The method as claimed in claiml, further comprises processing of Gel filtration is a technique in which the separation of components is based on the difference in molecular weight or size.
4. The method as claimed in claiml, further comprises processing of all the chromatography techniques and separates molecules on the basis of differences in size.
5. The method as claimed in claiml, further comprises processing of stationary phase used is a porous polymer matrix whose pores are completely filled with the solvent to be used as the mobile phase.
6. The method as claimed in claiml, further comprises processing the molecules in the sample are pumped through specialized columns containing such microporous packing material (gel).