WE CLAIM:
1. A genetically modified microorganism in which a nucleic acid encoding any
one of polypeptides described in (a) to (c) below is introduced or expression of the
polypeptide is enhanced:
5 (a) a polypeptide composed of an amino acid sequence represented by any
oneofSEQIDNOs: 1 to 6 and 213;
(b) a polypeptide composed of the same amino acid sequence as that
represented by any one of SEQ ID NOs: 1 to 6 and 213, except that one or several
amino acids are substituted, deleted, inserted, and/or added, and having an enzymatic
10 activity that catalyzes a reaction to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-
CoA;
(c) a polypeptide composed of an amino acid sequence with a sequence
identity of not less than 70% to the sequence represented by any one of SEQ ID
NOs: 1 to 6 and 213 and having an enzymatic activity that catalyzes a reaction to
15 reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA.
2. The genetically modified microorganism according to claim 1, wherein the polypeptide described in either (b) or (c) comprises a region with an amino acid sequence represented by SEQ ID NO: 212.
3. The genetically modified microorganism according to claim 2, wherein the
2 0 amino acid sequence represented by SEQ ID NO: 212 comprises a phenylalanine or
leucine residue as the 13th amino acid residue from the N terminus, a leucine or glutamine residue as the 15th amino acid residue from the N terminus, a lysine or asparagine residue as the 16th amino acid residue from the N terminus, a glycine or serine residue as the 17th amino acid residue from the N terminus, a proline or
2 5 arginine residue as the 19th amino acid residue from the N terminus, and preferably a
leucine, methionine, or valine residue as the 21st amino acid residue from the N terminus.

4. The genetically modified microorganism according to any one of claims 1 to
3. which is a genetically modified microorganism selected from the group consisting
of the genera Escherichia, Serratia , Hafnia, and Pseudomonas.
5. The genetically modified microorganism according to any one of claims 1 to
5 4. which has an ability to generate 3-oxoadipyl-CoA and coenzyme A from acetyl-
CoA and succinyl-CoA; and an ability to generate 3-hydroxyadipic acid from 3-hydroxyadipyl-CoA.
6. The genetically modified microorganism according to any one of claims 1 to
4, which has an ability to generate 3-oxoadipyl-CoA and coenzyme A from acetyl-
10 CoA and succinyl-CoA; an ability to generate 2,3-dehydroadipyl-CoA from 3-
hydroxyadipyl-CoA; and an ability to generate a-hydromuconic acid from 2,3-dehydroadipyl-CoA.
7. The genetically modified microorganism according to any one of claims 1 to
4, which has an ability to generate 3-oxoadipyl-CoA and coenzyme A from acetyl-
15 CoA and succinyl-CoA; an ability to generate 2,3-dehydroadipyl-CoA from 3-
hydroxyadipyl-CoA; an ability to generate adipyl-CoA from 2,3-dehydroadipyl-CoA; and an ability to generate adipic acid from adipyl-CoA.
8. A method of producing 3-hydroxyadipic acid, comprising culturing the
genetically modified microorganism according to any one of claims 1 to 5 in a
2 0 culture medium containing a carbon source as a material for fermentation.
9. A method of producing a-hydromuconic acid, comprising culturing the
genetically modified microorganism according to any one of claims 1 to 4 and 6 in a
culture medium containing a carbon source as a material for fermentation.
10. A method of producing adipic acid, comprising culturing the genetically
2 5 modified microorganism according to any one of claims 1 to 4 and 7 in a culture
medium containing a carbon source as a material for fermentation.
11. A method of producing one or more substances selected from the group

consisting of 3-hydroxyadipic acid, a-hydromuconic acid, and adipic acid,
comprising culturing a genetically modified microorganism in a culture medium
containing a carbon source as a material for fermentation, wherein a nucleic acid
encoding a polypeptide encoded by the 3-hydroxybutyryl-CoA dehydrogenase gene
5 of a microorganism of the genus Serratia, which forms a gene cluster with 5-
aminolevulinic acid synthase gene in the microorganism, is introduced or expression of the polypeptide is enhanced in the genetically modified microorganism.