HERBAL COSMETIC FORMULATION WITH AGE DEFYING PROPERTIES

We, Mr. Amit Kumar Assistant Professor, School of Pharmaceutical Sciences, IFTM University, Moradabad, Pin- 244102, Dr. Sushil Kumar, Professor and Director, School of Pharmaceutical Sciences, IFTM University, Moradabad, U.P, India, Pin- 244102, Mr. Shahbaz Khan, Assistant Professor, Pharmacology Research Laboratory, Pharmacy Academy, IFTM University, Moradabad, U.P, India Pin- 244102, Ms. Naina Rathore, School of Pharmaceutical Sciences, IFTM University, Moradabad, Pin- 244102, Ms. Sunaina Rathore, School of Pharmaceutical Sciences, IFTM University, Moradabad, Pin- 244102, Mr. Devdutt, Jagmeet Memorial College of Pharmacy, Bijnor Pin- 266725,

THE FOLLOWING SPECIFICATION PARTICULARLY DESCRIBES THE INVENTION AND THE MANNER IN WHICH IT IS TO BE PERFORMED

FIELD OF INVENTION:
The present invention belongs to the field of pharmaceutical science. More, particularly the invention pertains to synthesis and Biological Activity of novel age defying potential of cosmetic emulsions composed of Fennel seed oil, Coriander seed essential oil, Pumpkin seed oil, Olive oil, Neem oil, (oil phase) and Aloe vera gel (aqueous phase).
BACKGROUND OF THE INVENTION:
Skin ageing is the result of a continual deterioration process because of damage to cellular DNA and protein. The ageing process is classified into two distinct types i.e. “Sequential Skin Ageing” and “Photo Ageing”. Both types have distinct Clinical and Historical features. Sequential Skin Ageing is the universal and predictable process characterized by physiological alteration in skin function. In the ageing process keratinocytes are unable to form a functional stratum corneum and rate of formation of neutral lipids slows down, resulting in dry and pale skin with wrinkle. In contrast, Photo Ageing is caused by over exposure to UV rays from sunlight. It is characterized by dry, pale and sallow skin, displaying fine wrinkles as well as deep furrows caused by the disorganization of epidermal and dermal components associated with elastosis and heliodermatis.
Cosmetic products are used to protect against exogenous and endogenous harmful agents, and enhance the beauty and attractiveness of skin. The use of cosmetics not only developing an attractive external appearance, but towards achieving longevity of good health by reducing skin disorders .The synthetic or natural ingredients present in a skin care formulation that supports the health, texture, integrity of skin, moisturizing, maintaining the elasticity of skin by reduction of type I collagen, photo protection etc. This property of cosmetics is due to presence of ingredients in skin care formulations, because it helps to reduce the production of free radicals in the skin and manage the skin properties for a long time. The cosmetic products are the best choice to reduce skin disorders such as hyper pigmentation, skin ageing, skin wrinkling, rough skin texture etc. The demand of herbal cosmetic is rapidly expanding. Olive oil contains abundant amounts of Vitamin A. It acts as a very good anti-oxidant which slows down the process of ageing. Vitamin C produces collagen in the body which is essential protein for making our skin elastic and it also prevents wrinkles on skin.
There are a number of chemicals intended to slow the ageing process currently being studied in animal models. One type of research is related to the observed effects of a calorie restriction (CR) diet, which has been shown to extend lifespan in some animals. Based on that research, there have been attempts to develop drugs that will have the same effect on the ageing process as a caloric restriction diet, which are known as Caloric restriction mimetic drugs. Some drugs that are already approved for other uses have been studied for possible longevity effects on laboratory animals because of a possible CR-mimic effect; they include rapamycin, metformin and other geroprotectors. MitoQ, resveratrol and pterostilbene are dietary supplements that have also been studied in this context. Other attempts to create anti-ageing drugs have taken different research paths. One notable direction of research has been research into the possibility of using the enzyme telomerase in order to counter the process of telomere shortening. However, there are potential dangers in this, since some research has also linked telomerase to cancer and to tumor growth and formation.
Domestic and international most anti-aging cosmetics are that main effect is supported additive with biochemical preparation, synthetic compound at present. Along with the proposition of natural, safety and environmental requirement, green cosmetics are more and more favored by people.But existing on the market is the many pharmacological researches based on single medicinal material of cosmetics of interpolation with natural product or plant extract, does not have the guidance of Chinese medical theory, lacks the research of compound recipe. And the simple combination that mostly is the single Chinese herbal medicine extract of the anti-aging cosmetics of Chinese herbal medicine in the market, there is not the guidance of theory of Chinese medical science; Perhaps extraction process is the simple superposition of single medicinal material, cannot play complementary effect.
Cary L et al.,2000 teaches that ageing is a natural process that develops with various intrinsic and extrinsic factors involved. Thus, the recent advancement in the field of pharmacy and cosmetics have played an influential role in providing therapeutic effects for skin and preventing factors contributing to the ageing process. Various medicines, ointments, creams, etc either from chemicals or herbs alone or in combination of both have been designed. However, the exaggerated use of chemical agents may tend to cause some side effects, therefore the use of naturals as much as possible are empathized upon. The Pharmacognosy reveals various phyto chemicals and compounds having skin protecting and regenerating properties. For e.g. Phenolic compounds, Flavonoids, and Proanthocyanidins from plants are responsible for anti-oxidative activities of herbal products and explained by their chemical structure and ability to donate free electrons and hydrogen. The extracts of the tropical Cabbage palm fern (Polypodiumleucotomos) and Yerba mate (Ilex paragualiensis) have strong photo protective properties.
CN102614470A discloses an external Chinese herbal composition for treating sequela of apoplexy. The external Chinese herbal composition comprises the following Chinese herbal medicine raw materials by weight: 30 g of Chinese alangium, 10 g of pangolin, 20 g of earthworm, 15 g of scorpion, 20 g of turmeric root-tuber, 30 g of dutchmanspipe root, 30 g of flemingia, 30 g of lycopodium clavatum, 15 g of rhizoma typhonii, 20 g of astragali radix, 30 g of clematis root, 10 g of long-noded pit viper, 10 g of centipede, 8 g of asarum, 15 g of semen momordicae and 20 g of all grass or root of common calanthe. The external Chinese herbal composition has the advantages of low treatment cost and good treatment effect.
CN104546621A discloses an anti-aging skincare composition which is prepared from the following raw materials in percentage by weight: 20.0%-35.0% of a hydrolysis mirabalan extract, 20.0%-30.0% of a semen plantaginis extract, 10.0%-15.0% of a radix scutellariae extract, 1.0%-5.0% of collagen, 0.01%-0.05% of glutathione and the balance of deionized water. The preparation method of the anti-aging skincare composition comprises the following steps: (1) mixing deionized water evenly in percentage by weight, and heating to 45-55 DEG C; (2) adding the hydrolysis mirabalan extract, semen plantaginis extract, radix scutellariae extract, collagen and glutathione to the deionized water in the step 1), and further stirring at 45-55 DEG C at the speed of 40r/min for 10-20 minutes until mixing evenly; and (3) cooling to room temperature and adjusting the pH value to 5.5-7.0, so as to obtain the composition. The anti-aging skincare composition is prepared by compounding a plurality of anti-aging components; skin ageing can be significantly delayed; and the anti-aging skincare composition has certain anti-wrinkle and moisturizing effects.

In view of the foregoing, age defying potential of cosmetic emulsions composed of Fennel seed oil, Coriander seed essential oil, Pumpkin seed oil, Olive oil, Neem oil (oil phase) and Aloe vera gel (aqueous phase) is synthesized, wherein said composition meeting the following objectives are desired.
OBJECTIVE OF THE INVENTION:
1. It is an object of the invention to provide a herbal cosmetic emulsions having age defying potential comprises, in an effective concentration of Fennel seed oil, Coriander seed essential oil, Pumpkin seed oil, Olive oil, Neem oil and Aloe vera gel.

2. Another object of the present invention is to provide a herbal cosmetic emulsions having age defying potential as claimed in claim 1, wherein Fennel seed oil is 02 %W/V, Coriander seed essential oil is 02 %W/V, Pumpkin seed oil is 10 %W/V, Olive oil is 10 %W/V, Neem oil is 5 %W/V and Aloe vera gel is 70 %W/V.

3. Another object of the present invention is to provide a herbal cosmetic emulsions having age defying potential as claimed in claim 1, wherein Fennel seed oil is 02 %W/V, Coriander seed essential oil is 02 %W/V, Pumpkin seed oil is 10 %W/V, Olive oil is 10 %W/V, Neem oil is 5 %W/V and Aloe vera gel is 70 %W/V, wherein SPF value is 21.8.
DETAILED DESCRIPTION:
For the purpose of promoting an understanding of the principles of the invention, reference will now be made to the embodiment illustrated in the figures and specific language will be used to describe the same. It will nevertheless be understood that no limitation of the scope of the invention is thereby intended, such alterations and further modifications in the illustrated system, and such further applications of the principles of the invention as illustrated therein being contemplated as would normally occur to one skilled in the art to which the invention relates.

It will be understood by those skilled in the art that the foregoing general description and the following detailed description are exemplary and explanatory of the invention and are not intended to be restrictive thereof.

Reference throughout this specification to “an aspect”, “another aspect” or similar language means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, appearances of the phrase “in an embodiment”, “in another embodiment” and similar language throughout this specification may, but do not necessarily, all refer to the same embodiment.

The terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process or method that comprises a list of steps does not include only those steps but may include other steps not expressly listed or inherent to such process or method. Similarly, one or more devices or systems or elements or structures or components proceeded by "comprises... a" does not, without more constraints, preclude the existence of other devices or other systems or other elements or other structures or other components or additional devices or additional systems or additional elements or additional structures or additional components.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The system, methods, and examples provided herein are illustrative only and not intended to be limiting.

The terms “a” and “an” herein do not denote a limitation of quantity, but rather denote the presence of at least one of the referenced items.

The terms “having”, “comprising”, “including”, and variations thereof signify the presence of a component.

Now the present invention will be described below in detail with reference to the following embodiment.

Example-1
Materials
Essential oils and carrier oils such as Coconut oil, olive oil, Avocado oil etc, were procured from
Aromatic International (USA), emulsion stabilizers such as Aloe Vera gel, Xanthan gum etc will
be procured, from suitable suppliers. Nonionic surfactants were procured from suitable suppliers
(CDH, SD finechem etc.). All other Chemicals used will be of analytical grade.
Methods
GCMS analysis of essential oils
The identification of the components of the essential oil samples was carried out by gas chromatography equipment (Shimadzu QP-2010 Plus with Thermal Desorption System TD 20, Shimadzu, Kyoto, Japan); AB-INN Wax column (60 m length × 0.25 mm id × 0.25 µm thickness) was used under the conditions: column oven temperature was 50.0°C, injection temperature was 260.0°C, pressure was 69.0 kPa, total flow was 125.2 mL/min, and column flow was 1.21 mL/min. Injected oil sample volume was 0.1 µL, split ratio was 100.0, ion source temperature was 230.00°C, interface temperature was 270.0°C, and the mass spectrometer was scanned over the 40-650 m/z. Identification of individual oil component was done by comparing the mass data of individual oil component peaks with the standard library database .
Preparation of emulsions:-
Various emulsion formulations will be prepared using the hydrophile–lipophile balance (HLB) technique. Briefly, the oil phase comprising of essential oil(s), fixed oil, hydrophobic nonionic surfactants and other excipients will be mixed with the aqueous phase made up of emulsion stabilizers containing a hydrophilic nonionic surfactant using a mechanical stirrer until the mixture becomes homogenous (Table 1). The volume will be made up using deionized water, and the emulsions will be kept in wide mouth air tight containers.

Table: 1 Composition of emulsion formulation

Name of ingredients Concentration

(%W/V)
Formulation A1 Formulation A2
Fennel seed oil 02 02
Coriander seed oil 02 02
Virgin olive oil 10 ----
Pumpkin Seed Oil ---- 10
Neem Oil 5 5
Glycerin 5 5
Non ionic surfactants 7 7
Aloe vera gel 70 70

Example-2
Determination of Antioxidant activities of Cosmetic emulsions
1, 1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity
To evaluate the antioxidant capacity of formulations, the oil samples were allowed to react with methanolic solution of DPPH. DPPH is a stable nitrogen centered free radical which changes the color from dark violet to yellow in the presence of compounds that are capable of either donating hydrogen or transferring an electron. During the reaction, the reduction in DPPH can be monitored by the decrease in its absorbance at a characteristic wavelength (?max: 515-517 nm). The free radical scavenging activities of formulations were estimated by DPPH using the method described by Elmastas et al . Different dilutions of oil samples and standard (ascorbic acid) in the concentration range of 10-250 µg/mL were prepared in methanol. 0.1 mmol/L solution of DPPH was prepared in methanol, and 3 mL of this solution was added to the equal volume of diluted sample and standard solutions, mixed well, and the tubes were incubated for 30 minute in dark at 30°C. The absorbance of each dilution was measured at 517 nm using UV-Visible Spectrophotometer. DPPH radical scavenging capability was calculated using the following equation.

Where AbC is absorbance of control and AbS is absorbance of sample/standard. % Inhibition of each sample dilution was calculated and a graph was plotted to obtain a linear regression equation, by taking concentration (µg/mL) on x-axis and % Inhibition on y-axis. From the linear regression equation, IC50 value was calculated. IC50 value is the concentration of the sample required to scavenge 50% DPPH-free radical. The experiment was done in triplicate.
Nitric oxide scavenging activity
Sodium nitroprusside decomposes in aqueous physiological solution (pH-7.4) and generates nitric oxide. Nitric oxide reacts with oxygen under aerobic conditions and generated nitrite ions that can be estimated by using Griess reagent (1 part of napthylethylenediaminedihydrochloride in distilled water and 1 part of 1% sulfanilamide in 5% phosphoric acid). Nitric oxide scavengers compete with oxygen leading to reduced production of nitrite ions. In this method, 10 mmol/L sodium nitroprusside was prepared in phosphate buffer (pH 7.4) and 2 mL of this solution was added to oil sample and standard (ascorbic acid) dilutions (10-250µg/mL) in methanol. The tubes were incubated at 25°C for 2 hours. After incubation, 0.5 mL Griess reagent was added to the incubated tubes and absorbance was measured at 546 nm using UV-Visible Spectrophotometer. The amount of nitric oxide radical was calculated by following the equation.

Where AbC is absorbance of control and AbS is absorbance of sample/standard. % Inhibition of each sample dilution was calculated and a graph was plotted to obtain a linear regression equation, by taking concentration (µg/ml) on x-axis and % Inhibition on y-axis. From the linear regression equation, IC50 value was calculated. IC50 value is the concentration of the sample required to scavenge 50% Nitric oxide-free radical. The experiment was done in triplicate.
While the invention has been described with respect to specific compounds which include presently preferred modes of carrying out the invention, those skilled in the art will appreciate that there are numerous variations and permutations of the above described embodiments that fall within the spirit and scope of the invention. It should be understood that the invention is not limited in its application to the details of construction and arrangements of the components set forth herein.
Variations and modifications of the foregoing are within the scope of the present invention. Accordingly, many variations of these embodiments are envisaged within the scope of the present invention.
The foregoing descriptions of specific embodiments of the present invention have been presented for purposes of description. They are not intended to be exhaustive or to limit the present invention to the precise forms disclosed, and obviously many modifications and variations are possible in light of the above teaching.
The embodiments were chosen and described in order to best explain the principles of the present invention and its practical application, and to thereby enable others skilled in the art to best utilize the present invention and various embodiments with various modifications as are suited to the particular use contemplated. It is understood that various omissions and substitutions of equivalents are contemplated as circumstances may suggest or render expedient, but such omissions and substitutions are intended to cover the application or implementation without departing from the spirit or scope of the present invention.

We claim
1. A herbal cosmetic emulsions having age defying potential comprises, in an effective concentration of Fennel seed oil, Coriander seed essential oil, Pumpkin seed oil, Olive oil, Neem oil and Aloe vera gel.

2. The herbal cosmetic emulsions having age defying potential as claimed in claim 1, wherein Neem oil is in oil phase.

3. The herbal cosmetic emulsions having age defying potential as claimed in claim 1, wherein Aloe vera gel is in aqueous phase.

4. The herbal cosmetic emulsions having age defying potential as claimed in claim 1, wherein Fennel seed oil is 02 %W/V, Coriander seed essential oil is 02 %W/V, Pumpkin seed oil is 10 %W/V, Olive oil is 10 %W/V, Neem oil is 5 %W/V and Aloe vera gel is 70 %W/V.

5. The herbal cosmetic emulsions having age defying potential as claimed in claim 1, wherein emulsion further comprises 7%W/V Nonionic surfactants and 5%W/V Glycerin.

6. The herbal cosmetic emulsions having age defying potential as claimed in claim 1, wherein SPF value is 21.8.

ABSTRACT
HERBAL COSMETIC FORMULATION WITH AGE DEFYING PROPERTIES

The present invention belongs to the field of pharmaceutical science. More, particularly the invention pertains to A herbal cosmetic emulsions having age defying potential comprises, in an effective concentration of Fennel seed oil, Coriander seed essential oil, Pumpkin seed oil, Olive oil, Neem oil and Aloe vera gel. The emulsion further comprises 7%W/V Nonionic surfactants and 5%W/V Glycerin. The herbal cosmetic emulsions having age defying potential has SPF value of 21.8.

Claims:1. A herbal cosmetic emulsions having age defying potential comprises, in an effective concentration of Fennel seed oil, Coriander seed essential oil, Pumpkin seed oil, Olive oil, Neem oil and Aloe vera gel.

2. The herbal cosmetic emulsions having age defying potential as claimed in claim 1, wherein Neem oil is in oil phase.

3. The herbal cosmetic emulsions having age defying potential as claimed in claim 1, wherein Aloe vera gel is in aqueous phase.

4. The herbal cosmetic emulsions having age defying potential as claimed in claim 1, wherein Fennel seed oil is 02 %W/V, Coriander seed essential oil is 02 %W/V, Pumpkin seed oil is 10 %W/V, Olive oil is 10 %W/V, Neem oil is 5 %W/V and Aloe vera gel is 70 %W/V.

5. The herbal cosmetic emulsions having age defying potential as claimed in claim 1, wherein emulsion further comprises 7%W/V Nonionic surfactants and 5%W/V Glycerin.

6. The herbal cosmetic emulsions having age defying potential as claimed in claim 1, wherein SPF value is 21.8.