Specification
(1) FIELD OF THE INVENTION
The present invention generally relates to the field ofa functional carbonated ready-todrink
formulation. The invention, particularly relates toa functional carbonated ready-todrink
formulation comprising seven ingredients WithaniaSomnifera; BacopaMonneiri;
Asphaltum; ChlorophytumBorivilianum; Panax; TribulusTerrestris; and Asparagus
Racemosus.
(2) BACKGROUND OF THE INVENTION
A soft drink is a drink that typically contains carbonated water, a sweetener, and a natural
or artificial flavoring. The sweetener may be sugar, high-fructose corn syrup, fruit juice, or
some combination of these. Soft drinks may also contain caffeine, colorings, preservatives,
and other ingredients. Further, if more herbs are added to the soft drink then is known as
"herbal soft drink". Many times, "herbal soft drink" can also refer as "functional
beverage". The functional beverage functional beverage is a non-alcoholic drink. Some
include ingredients like herbs, vitamins, minerals, amino acids, or additional raw fruit or
vegetables. Examples of functional beverages include sports and performance drinks,
energy drinks, ready to drink (RTD) teas, enhanced fruit drinks, soy beverages, and
enhanced water. Functional beverages have become popular among people who want
specific health benefits from their foods and beverages. Both convenience and health have
been identified as important factors in consumers' decision-making about food and
beverage purchases. Functional drinks are advertised as having various health benefits. For
example, some claim to improve heart health, immunity, digestion, and joint health, while
others promote themselves as satiating and energy-boosting.
A number of different types of herbal or functional formulations are available in the prior
art. Prior art document, EP1572221A1 discloses a novel herbal soft drink comprising
decoction of plants selected from Sidasps., Vitisvinifera, Withaniasomnifera,
Boerhaaviadiffusa and Tinosporacordifolia for the protection and prevention of health and
in particular, but not exclusively with antioxidant, immunoenhancing, hepatoprotective,
cardiotonic, diuretic, digestive, choleretic, nervine relaxant properties. Another prior art
document, JP2013183723A discloses a beverage containing reed leaf powder,
ashwagandha leaf powder and rice powder. Yet another prior art document, US5417994
discloses microstaoie son annK oeverages containing at least two eaioie acias cnosen trom
phosphoric, citric and malic acids as a microstabilizer, in combination with water, a flavor
system of one or more flavor extracts and distillates, and a sweetener, such as HFCS. Yet
another prior art document, US20090175964 discloses a composition comprising theanine
and a herb selected from Shankhpushpi and/or Shatavari for use as a medicament in the
treatment or prevention of a disease associated with reduced immunity in an individual.
The disease may, for example, be cold or influenza. Yet another prior art document,
KR101367885 discloses a composition comprising the extract of combined herb of Panax
ginseng and Vitis genus plant treating and preventing neuro-degenerative disease and
enhancing memory power. Another prior art document, US20060062863discloses
compositions for anti-obesity, health-restorative and health promotional benefits to
mammals, more particularly humans. This composition is obtained from higher plants
belonging to genus Chlorophytum. The extraction process for obtaining such composition,
as well as pharmaceutical, nutritional and veterinary use products thereof, are also
disclosed. Yet another prior art document, WO2015142611 discloses a non-carbonated preoperative
beverage containing Panax ginseng. Non-patent literature prior art document
discloses a preparation of Green Tea and Brahmi extract powder hard vegetable capsule by
manual filling machine.The hard vegetable capsule is used for healthy brain (see:
http://iippr.humaniournals.coni/fomiulation-and-evaluation-of-hard-vegetable-capsule-fo
healthv-brain-using-powder-of-green-tea-and-brahmi-extract/ ). Yet another prior art
document, US20160235801 discloses a method and formulation for fluids, such as drinking
water, containing plant phytochemicals, brahmi extract. These phytochemicals have been
shown to exhibit antioxidant properties, promote memory and provide additional health
benefits, as well as replace bottled water or other fluids as a means to ensure proper
hydration. Another prior art document, WO2009054002 discloses a soft drink based on
Indian gooseberry extract and a process of production thereof comprising the following
steps: - washing and drying of fresh Indian gooseberries, - cutting the berries into small
pieces and further drying for a couple of weeks, - extracting the dried berries with water, -
filtering, boiling and pasteurizing the extract, - adding sugar or sweeteners and
preservatives, - treating with UV-light and filtering, - carbonating, and - packaging of the
resulting soft drink.
However, above mentioned references and many other similar references have one or more
of the following shortcomings: (a) containing only few of the ingredients; (b) noncarbonated drinks/beverages; (cj expensive; (d) having some side ettects; (e) having only
fewer or specific health benefits; (f) having higher sugar content; and (g) not in the form of
ready-to-drink.There remains a constant need in society for an innovative and novel
beverages or drinks containing herbal ingredients.
(3) SUMMARY OF THE INVENTION
In the view of the foregoing disadvantages inherent in the known types of herbal soft
drinknow present in the prior art, the present invention provides a functional carbonated
ready-to-drink formulation. As such, the general purpose of the present invention, which
will be described subsequently in greater detail, is to provide a new and improved
functional carbonated ready-to-drink formulation comprising at least five ingredients
selected from the group ofherbs WithaniaSomnifera; BacopaMonneiri; Asphaltum;
ChlorophytumBonvilianum; Panax; TribulusTerrestris; and Asparagus Racemosus.
An object of the invention is to provide a functional carbonated ready-to-drink formulation
comprising at least five ingredients selected from the group of WithaniaSomnifera;
BacopaMonneiri; Asphaltum; ChlorophytumBonvilianum; Panax; TribulusTerrestris; and
Asparagus Racemosus.
It is another object of the present invention to provide the functional carbonated ready-todrink
formulation, wherein w/w ratio of WithaniaSomnifera :BacopaMonneiri is 3:2.
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein w/w ratio of Asphaltum :ChlorophytumBorivilianum is 1:3.
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein w/w ratio of Panax : Asparagus Racemosus is 0.5:1.
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein w/w ratio of WithaniaSomnifera .TribulusTerrestris is 2:1.
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein w/w ratio of TribulusTerrestris :BacopaMonneiri is 0.75:1.
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein w/w ratio of Panax : Asparagus Racemosus :
TribulusTerrestris is 1:2:2.5.
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, further comprises aloevera, a food-grade gum, and sugar.
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein the food-grade gum is selected from the group consisting of
guar gum, acacia gum, xanthan gum, tragacnath, locust bean gum, carrageenan,
arabinogalactam, tara, gellan, or mixture thereof.
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein the food-grade gum is selected from the group consisting of
xanthan gum, guar gum or mixture thereof.
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein the ready-to-drink formulation has at least 5 degree Brix
sugar content.
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein the ready-to-drink formulation having the sugar content in
the range of 5 to 25 degree Brix,
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein the ready-to-drink formulation having the sugar content in
the range of 9 to 12 degree Brix.
It is also object of the present invention to provide a functional carbonated ready-to-drink
formulation comprising: WithaniaSomnifera is in range of about 1080 to 1320 mg per liter
of said ready-to-drink formulation; BacopaMonneiri is in range of about 720 to 880 mg
per liter of said ready-to-drink formulation; Asphaltum is in range of about 252 to 308 mg
per liter of said ready-to-drink formulation; ChlorophytumBonvilianum is in range of
5 of 26
• Atty. doc. No. 001
y about 756 to 924 mg per liter of said ready-to-drink formulation; Panax is uiYange of abouf w "^ ""*•
216 to 264 mg per liter of said ready-to-drink formulation; TribulusTerrestris is in range of
about 540 to 660 mg per liter of said ready-to-drink formulation; and Asparagus
Racemosus is in range of about 432 to 528 mg per liter of said ready-to-drink formulation.
5
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein WithaniaSomnifera is 1200 mg per liter of said ready-todrink
formulation; BacopaMonneiri is 800 mg per liter of said ready-to-drink formulation;
Asphaltum is 280 mg per liter of said ready-to-drink formulation;
10 ChlorophytumBorivilianum is 840 mg per liter of said ready-to-drink formulation; Panax is
240 mg per liter of said ready-to-drink formulation; TribulusTerrestris is 600 mg per liter
of said ready-to-drink formulation; and Asparagus Racemosus is 480 mg per liter of said
ready-to-drink formulation.
15 Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, further comprises aloevera, a food-grade gum, and sugar.
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein the food-grade gum is selected from the group consisting of
20 guar gum, acacia gum, xanthan gum, tragacnath, locust bean gum, carrageenan,
arabinogalactam, tara, gellan, or mixture thereof.
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein the food-grade gum is selected from the group consisting of
xanthan gum, guar gum or mixture thereof.
25
Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein the ready-to-drink formulation has at least 5 degree Brix
sugar content.
30 Yet it is another object of the present invention to provide the functional carbonated readyto-
drink formulation, wherein the ready-to-drink formulation having the sugar content in
the range of 5 to 25 degree Brix.
6 of 26
• Yet it is another object of the present invention to"pfbvide lhTfun^tTonarc^6bna!ed'rea3y^
to-drink formulation, wherein the ready-to-drink formulation having the sugar content in
the range of 9 to 12 degree Brix.
In this respect, before explaining at least one embodiment of the invention in detail, it is to
be understood that the invention is not limited in its application to the details of
construction and to the arrangements of the components set forth in the following
description or illustrated in the drawings. The invention is capable of other embodiments
and of being practiced and carried out in various ways. Also, it is to be understood that the
phraseology and terminology employed herein are for the purpose of description and
should not be regarded as limiting.
These together with other objects of the invention, along with the various features of
novelty which characterize the invention, are pointed out with particularity in the
disclosure. For a better understanding of the invention, its operating advantages and the
specific objects attained by its uses, reference should be had to the accompanying drawings
and descriptive matter in which there are illustrated preferred embodiments of the
invention.
4) DETAILED DESCRIPTION OF THE INVENTION
In the following detailed description, reference is made to the accompanying drawings
which form a part hereof, and in which is shown by way of illustration specific
embodiments in which the invention may be practiced. These embodiments are described
in sufficient detail to enable those skilled in the art to practice the invention, and it is to be
understood that the embodiments may be combined, or that other embodiments may be
utilized and that structural and logical changes may be made without departing from the
spirit and scope of the present invention. The following detailed description is, therefore,
not to be taken in a limiting sense, and the scope of the present invention is defined by the
appended claims and their equivalents.
The description will clearly enable one skilled in the art to make and use the invention, and
describes several embodiments, adaptations, variations and alternatives and uses of the
invention, including what we presently believe is the best mode for carrying out the
invention. It is to be clearly understood that routine variations and adaptations can be made
to the invention as described, and such variations and adaptations squarely fall within the
spirit and scope of the invention.
In other words, the invention is described in connection with such embodiments, but the
invention is not limited to any embodiment. The scope of the invention is limited only by
the claims and the invention encompasses numerous alternatives, modifications and
equivalents. Numerous specific details are set forth in the following description in order to
provide a thorough understanding of the invention. These details are provided for the
purpose of example and the invention may be practiced according to the claims without
some or all of these specific details. For the purpose of clarity, technical material that is
known in the technical fields related to the invention has not been described in detail so
that the invention is not unnecessarily obscured.
The present invention will now be described in brief with reference to the accompanying
drawings. Now, refer in more detail to the exemplary drawings for the purposes of
illustrating non-limiting embodiments of the present invention.
? As used herein, the term "comprising" and its derivatives including "comprises" and
"comprise" include each of the stated integers or elements but does not exclude the
inclusion of one or more further integers or elements.
• As used herein, the singular forms "a", "an", and "the" include plural referents unless the
context clearly dictates otherwise. For example, reference to "a substituent" encompasses a
single substituent as well as two or more substituents, and the like.
• As used herein, the terms "for example", "for instance", "such as", or "including" are
meant to introduce examples that further clarify more general subject matter. Unless
otherwise specified, these examples are provided only as an aid for understanding the
applications illustrated in the present disclosure, and are not meant to be limiting in any
fashion.
• As used herein, the term "ready-to-drink formulation" shall refer to "beverage", "drink",
"soft drink", "liquid", "cola", "soda", "carbonated drink", "RTD", "Ready-to-take",
v "arinjcaDie", "liquid taKen oy mouur, or comoination or mixtures or airrerent Torms ur
/
objects.
• As used herein, the term "carbonated" shall refer to "carbon dioxide purged!',
"carbon dioxide included", "carbon dioxide mixed",
"carbon dioxide containing", "soda", "C02", or combination or mixture of different terms
of object.
Carbonated water is a primary ingredient of soft drinks. Carbonated water was created by
Joseph Priestly in the year 1767. Carbonated formulation is water into which low levels of
pressurized carbon dioxide has been dissolved. Carbonated drink has long been considered
a wholesome alternative to regular water. Carbonated drink has that characteristic fizz and
slightly sour flavor which is why it is preferred by those who dislike the flat taste of plain
still water. And aside from it being a zero-calorie drink, soda water offers a handful of
other health benefits as well. Carbonated drink has many benefits for the gastrointestinal
tract. Carbonated drink eases stomachaches, quells nausea and has been proven to alleviate
constipation.
WithaniaSomnifera (Ashwagandha) is to restore strength and vigor to those lacking in
dynamism and energy, such as convalescents, Ashwagandha stimulates the immune system
and promotes the healthy functioning of organism, like adaptogenic plants.
WithaniaSomnifera benefits the entire body and mind, and. its range uses is very broad. It
protects the body from numerous diseases, helps to prevent cancer and it also possesses
aphoridisiac virtues, acting as a powerful regulator. WithaniaSomnifera is a plant that
effectively combats all forms of stress and promotes sleep and quality of sleep.
BacopaMonneiri (Brahmi) or extracts thereof have been found to sedate, potentiate
barbiturate hypnosis, and improve learning on various tasks; glutamatergic mechanisms
may be involved. 16 Ingredients of Brahmi that may be responsible for its cognitive effects
include the saponinsbacosides A and B. 18-20 Brahmi extracts have been reported to be
non-toxic, non-teratogenic and non-mutagenic in rats and monkeys; single and multiple
dosing studies in healthy human volunteers have also elicited no adverse effects.
a --ii* T
/Yspnanum ^mmjn; nas oeen wiueiy useu as a iraumonai meuicine anu nas many oeneius. ~ 'M ^
Asphaltum has many beneficial properties such as anti-ageing, regenerative properties,
energy synthesis, blood purifications, increase in sexual potency, prevents and cures
diabetes, solves bone related problems, and spiritual enlightenment.
ChlorophytumBorivilianum (SafedMusli), also called White Musli, is a potent aphrodisiac
and string adaptogenic herb used in ayurveda and herbal science. It is effective for
improving male performance, inducing spermatogenesis and providing strength to the
muscles.ChlorophytumBorivilianum is one of important herb in ayurveda, which provides *
highly beneficial and advisable for diseases like osteoarthritis, male weakness, reduced
vitality, and diabetes in thin or weak people, cancer, muscular weakness, fatigue,
oligospermia, bodybuilding etc.
Panax (ginseng) includes any one of 11 species of slow-growing perennial plants with
fleshy roots, belonging to the genus Panax of the family Araliaceae. Panax ginseng is
particularly recommended for those lacking in energy, as it is a very strong and healthy
stimulant. Panax ginseng contains active complex carbohydrates called ginsenosides, each
with the capacity to stimulate or soothe the nervous system, balance metabolic processes,
decrease blood sugar, improve muscle tone and stimulate the endocrine system. It enhances
the brain's adrenocorticotropic hormone (ACTH) activity without involving the adrenal
glands.
TribulusTerrestris(gokhru) is a plant from ayurveda where the root and fruits are used for
male virility and general vitality, respectively. The roots enhance libido and sexual wellbeing
without affecting testosterone while the fruits appear to be potently protective of
organ function.
Asparagus racemosus (Shatavari) has been widely used in folk medicine and is today a
highly commercially important target species. Shatavari (sometimes spelt as Shatawari),
also known as Shatamuli includes the herb Asparagus racemosus. It belongs to the family
of Liliaceae. The plant is found throughout central and southern India and in lower
Himalayas. The herb contains steroidal saponins. It has been traditionally used for making
general tonics and is especially used as a tonic for female reproductive function and to
.Atty. doc. No. 001
/
• 9 •& / T ^ 7 - f t f i - Q f t V f c ! •-"1 S I *-»
increase lactation. Additionally it has been used for providing relief from dysentery,
spasms, and also for antioxidant, immune stimulant, and antibacterial benefits.
10
Materials Used
The following materials have been used in the preparation of Functional Carbonated
Ready-to-drink Formulation.
WithaniaSomnifera liquid extract
The liquid extracts of WithaniaSomniferaor Ashwagandhawas obtained from the supplier.
The WithaniaSomnifera liquid extract was extracted from its root. The WithaniaSomnifera
liquid extract is of dark brown color and has passed the standard hysic chemical tests,
heavy metals content tests and microbiological tests which are shown in the following
table.
Sr, No.
A
1.
2.
3.
4.
5.
6.
7.
B.
8.
9.
10.
11.
c
12.
13.
14.
15.
16.
17.
Test
Physico chemical tests
Color
Odor
Water solubility
Propylene glycol stability
pH (1% w/v aqueous solution)
Specific gravity (20 UC)
Dry residue (% - 105 UC)
Heavy Metals
Lead
Arsenic
Mercury
Cadmium
Microbiological Tests
Total Aerobic Bacteria
Yeast and Moulds
Salmonella sp.
Escherichia Coli
Pseudomonas aeroginosa
Staphylococcus aureus
Results
Dark brown liquid
Characteristic
Compiles
Compiles
5.21
1.036
1.27%
Compiles
Compiles
Compiles
Compiles
Compiles
Compiles
Absent
Absent
Absent
Absent
Standards
Dark brown liquid
Characteristic
Soluble
Soluble
4.0-7.0
1.000-1.050
1 % - 2 %
10 ppm
3 ppm
1 ppm
0.3 ppm
NMTlOOOcfu/gm
NMTlOOcfu/gm
Absent
Absent
Absent
Absent
11 of 26
. Atty. doc. No. 001
/
18.
\ a
Total Coliforms
JTm T fci "1 3 ff^
Compiles
i i i • i
NMTlOcfu/gm
BacopaMonneiriLiquid Extract
The liquid extracts of BacopaMonnerior Brahmiwas obtained from the supplier. The
BacopaMonneri liquid extract was extracted from its whole plant. The BacopaMonneiri
liquid extract is of brown color and has passed the standard hysic chemical tests, heavy
metals content tests and microbiological tests which are shown in the following table.
Sr. No.
A
1.
2.
'3.
4.
5.
6.
7.
B.
8.
9.
10.
11.
C.
12.
13.
14.
15.
16.
17.
18.
Test
Physico chemical tests
Color
Odor
Water solubility
Propylene glycol stability
pH (1% w/v aqueous solution)
Specific gravity (20 UC)
Dry residue (%-105 UC)
Heavy Metals
Lead
Arsenic
Mercury
Cadmium
Microbiological Tests
Total Aerobic Bacteria
Yeast and Moulds
Salmonella sp.
Escherichia Coli
Pseudomonas aeroginosa
Staphylococcus aureus
Total Coliforms
Results
Brown color liquid
Characteristic
Compiles
Compiles
5.5
1.02
3.4%
Compiles
Compiles
Compiles
Compiles
Compiles
Compiles
Absent
Absent
Absent
Absent
Compiles
Standards
Brown color liquid
Characteristic
Soluble
Soluble
4.0-7.0
1.000-1.050
1 % - 5 %
10 ppm
3 ppm
1 ppm
0,3 ppm
NMT lOOOcfu/gm
NMTlOOcfu/gm
Absent
Absent
Absent
Absent
NMTlOcfu/gm
Asphaltum Liquid Extract
The liquid extracts of Asphaltumor Shilajitwas obtained from the supplier. The Asphaltum
liquid extract was extracted from its stone. The Asphaltum liquid extract is of brown color
12 of 26
,Atty. doc. No. 001
\ and has passed the standard hysic chemical tests, neavy^mefafs" "cdnfent tetfsJan8* ***•*-*"
y
microbiological tests which are shown in the following table.
Sr. No.
A
1.
2.
3.
4.
5.
6.
7.
B.
8.
9.
10.
11.
C.
12.
13.
14.
15.
16.
17.
18.
Test
Physico chemical tests
Color
Odor
Water solubility
Propylene glycol stability
pH (1% w/v aqueous solution)
Specific gravity (20 °C)
Dry residue (%-105 UC)
Heavy Metals
Lead
Arsenic
Mercury
Cadmium
Microbiological Tests
Total Aerobic Bacteria
Yeast and Moulds
Salmonella sp.
Escherichia Coli
Pseudomonas aeroginosa
Staphylococcus aureus
Total Coliforms
Results
Brown color liquid
Characteristic
Compiles
Compiles
5.68
1.032
1.08%
Compiles
Compiles
Compiles
Compiles
Compiles
Compiles
Absent
Absent
Absent
Absent
Compiles
Standards
Brown color liquid
Characteristic
Soluble
Soluble
4.0^7.0
1.000-1.050
0.5% - 1 . 5%
10 ppm
3 ppm
1 ppm
0.3 ppm
NMTlOOOcfu/gm
NMTlOOcfii/gm
Absent
Absent
Absent
Absent
NMTlOcfu/gm
ChlorophvtumBorivilianum Liquid Extract
The liquid extracts of ChlorophytumBorivilianumor Musliwas obtained from the supplier.
The ChlorophytumBorivilianumliquid extract was extracted from its root. The
ChlorophytumBorivilianum liquid extract is of brown color transparent liquid and has
passed the standard hysic chemical tests, heavy metals content tests and microbiological
tests which are shown in the following table.
Sr. No. Test Results Standards
13 of 26
,Atty. doc. No. 001
\ A
1.
2.
3.
4.
5.
6.
7.
B.
8.
9.
10.
11.
C.
12.
13.
14.
15.
16.
17.
18.
a
Physico chemical tests
Color
Odor
Water solubility
Propylene glycol stability
pH (1% w/v aqueous solution)
Specific gravity (20 °C)
Dry residue (% - 105 °C)
Heavy Metals
Lead
Arsenic
Mercury
Cadmium
Microbiological Tests
Total Aerobic Bacteria
Yeast and Moulds
Salmonella sp.
Escherichia Coli
Pseudomonas aeroginosa
Staphylococcus aureus
Total Coliforms
e - * T *• v r?i .^ - H
Brown coloi
transparent liquid
Characteristic
Compiles
Compiles
5.14
1.024
0.86 %
Compiles
Compiles
Compiles
Compiles
Compiles
Compiles
Absent
Absent
Absent
Absent
Compiles
f?i - &s fTs 'T fct ™1 53 rfl
-i, n . H -B • '• » a « a *. -a
Brown coloi
transparent liquid
Characteristic
Soluble
Soluble
4.0-7.0
1.000-1.050
0.5%-1.5%
lOppm
3 ppm
1 ppm
0.3 ppm
NMTlOOOcfu/gm
NMTlOOcfu/gm
Absent
Absent
Absent
Absent
NMTlOcfu/gm
rf3^ 'Jt T
Panax Liquid Extract
The liquid extracts of Panaxor Ginsengwas obtained from the supplier. The Panax
Ginsenghquid extract was extracted from its root. The Panax Ginseng liquid extract is of
brown color liquid and has passed the standard hysic chemical tests, heavy metals content
tests and microbiological tests which are shown in the following table.
Sr. No.
A
1.
2.
3.
Test
Physico chemical tests
Color
Odor
Water solubility
Results
Brown color liquid
Characteristic
Compiles •
Standards
Brown color liquid
Characteristic
Soluble
14 of 26
,Atty. doc. No. 001
/
4.
5.
6.
7.
B.
8.
9.
10.
11.
c.
12.
13.
14.
15.
16.
17.
18.
Propylene glycol stability
pH (1% w/v aqueous solution)
Specific gravity (20 UC)
Dry residue (%-105 UC)
Heavy Metals
Lead
Arsenic
Mercury
Cadmium
Microbiological Tests
•
Total Aerobic Bacteria
Yeast and Moulds
Salmonella sp.
Escherichia Coli
Pseudomonas aeroginosa
Staphylococcus aureus
Total Coliforms
Compiles
4.68
1.02
3.4%
Compiles
Compiles
Compiles
Compiles
Compiles
Compiles
Absent
Absent
Absent
Absent
Compiles
Soluble
4.0-7.0
1.000-1.050
1 % - 5 %
lOppm
3 ppm
1 ppm
03 ppm
NMTlOOOcfu/gm
NMTlOOcfu/gm
Absent
Absent
Absent
Absent
NMTlOcfu/gm
W >-jt -&.
Asparagus Racemosus
The liquid extracts of Asparagus Racemosusor Shatavariwas obtained from the supplier.
The Asparagus Racemosushquid extract was extracted from its root. The Asparagus
Racemosus liquid extract is of brown color liquid and has passed the standard hysic
chemical tests, heavy metals content tests and microbiological tests which are shown in the
following table.
Sr. No.
A
1.
2.
3.
4.
5.
6.
7.
Test
Physico chemical tests
Color
Odor
Water solubility
Propylene glycol stability
pH (1% w/v aqueous solution)
Specific gravity (20 UC)
Dry residue (% - 105 UC)
Results
Brown color liquid
Characteristic
Compiles
Compiles
3.5
1.028
5.60 %
Standards
Brown color liquid
Characteristic
Soluble
Soluble
3.5-7.0
1.000-1.050
l % - 5 . 6%
15 of 26
. Atty. doc. No. 001
\ B.
8.
9.
10.
11
C.
12,
13
14.
15
16.
17,
18
Heavy Metals
Lead
Arsenic
Mercury
Cadmium
Microbiological Tests
Total Aerobic Bacteria
Yeast and Moulds
Salmonella sp.
Escherichia Coli
Pseudomonas aeroginosa
Staphylococcus aureus
Total Coliforms
•9 - e ••
•a. n — -
# T
ft,. 1
Compiles
Compiles
Compiles
Compiles
Compiles
Compiles
Absent
Absent
Absent
Absent
Compiles
lOppm
3 ppm
1 ppm
0.3 ppm
NMTlOOOcfu/gm
NMTlOOcfu/gm
Absent
Absent
Absent
Absent
NMTlOcfu/gm
Aloevera
The liquid extracts of Aloveralndicaor Aloeverawas obtained from the supplier. The
Aloeveraliquid extract was extracted from its lead. The Aloevera liquid extract is of clear
transparent color liquid and has passed the standard hysic chemical tests, heavy metals
content tests and microbiological tests which are shown in the following table.
Sr. No,
A
1.
2.
3.
4.
5.
6.
7.
B.
8.
9.
10.
Test
Physico chemical tests
Color
Odor
Water solubility
Propylene glycol stability
pH (1% w/v aqueous solution)
Specific gravity (20 °C)
Dry residue (%-105 UC)
Heavy Metals
Lead
Arsenic
Mercury
Results
Clear Colo
Transparent liquid
Characteristic
Compiles
Compiles
4.28
1.032
5.60 %
Compiles
Compiles
Compiles
Standards
Clear Colo]
Transparent liquid
Characteristic
Soluble
Soluble
3.0-7.0
1.000-1.050
l % - 5 . 6%
10 ppm
3 ppm
1 ppm
16 of 26
Atty. doc. No. 001
10
15
11.
c.
12.
13.
14.
15.
16.
17.
18.
Cadmium
Microbiological Tests
Total Aerobic Bacteria
Yeast and Moulds
Salmonella sp.
Escherichia Coli
Pseudomonas aeroginosa
Staphylococcus aureus
Total Coliforms
Compiles
Compiles
Compiles
Absent
Absent
Absent
Absent
Compiles
0.3 ppm
NMT1000cfii/gm
NMTlOOcfWgm
Absent
Absent
Absent
Absent
NMTlOcfu/gm
All materials are stored at cool, dry and dark clean space. Storage temperature not
exceeding 25 °C.
EXAMPLE:
Example 1: 250 ml Functional Carbonated Readv-to-drink Formulation with 10
degree brix with standard herbal ingredients content
A preparation method of 250 ml functional carbonated ready-to-drink formulation
according to the present invention is disclosed in detailed as below.
In a first container 2.25 mg of a mixture of guar gum and xanthan gum (1:1) was taken and
then 3.75 ml of water added to the gum mixture. Gum mixturemixed with water was heated
around the temperature 70 °C to completely dissolve the gum in water.
In a second container a mixture of seven herbal ingredients and other components were
taken. The exact quantity of the each ingredient and other component were taken as:
WithaniaSomnifera
BacopaMonneiri
Asphaltum
ChlorophytumBorivilianum
Panax
TribulusTerrestris
Asparagus Racemosus
Aloevera
300 mg
200 mg
70 mg
210 mg
60 mg
150 mg
120 mg
3 ml
20
The above described compositions were mixed in the second container with around 70 ml
of water.
In a next step the gum solution and the water containing herbal ingredients were mixed
together and make a total solution. This total solution was added to the individual bottles.
17 of 26
Atty. doc. No. 001
Documents
Application Documents
| # |
Name |
Date |
| 1 |
201711012355-Form 5-060417.pdf |
2017-04-07 |
| 2 |
201711012355-Form 3-060417.pdf |
2017-04-07 |
| 3 |
201711012355-Form 2(Title Page)-060417.pdf |
2017-04-07 |
| 4 |
201711012355-Form 1-060417.pdf |
2017-04-07 |
| 5 |
201711012355-Form 9-010517.pdf |
2017-05-02 |
| 6 |
201711012355-Form 18-010517.pdf |
2017-05-02 |
| 7 |
201711012355-FER.pdf |
2021-10-17 |
Search Strategy
| 1 |
SearchstrategyTKDLE_29-10-2020.pdf |
| 2 |
searchstrategyPLE_29-10-2020.pdf |