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Herbal Drink

Abstract: This invention related to a functional carbonated ready-to-drink formulation.The invention, particularly relates to a functional carbonated ready-to-drink formulation comprising seven ingredients WithaniaSomnifera; BacopaMonneiri; Asphaltum; ChlorophytumBorivilianum; Panax; TribulusTerrestris; and Asparagus Racemosus. The functional carbonated ready-to-drink formulation further also includes gum mixture and aloevera. The functional carbonated ready-to-drink formulation has at least 5 degree brix sugar content. The advantages of taking this innovative functional carbonated ready-todrink formulation over other available drinks is that this contains mixture of herbs that are proven to provide energy naturally, acts as immune boosters, anti-stress, better blood circulation etc.

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Patent Information

Application #
Filing Date
06 April 2017
Publication Number
19/2017
Publication Type
INA
Invention Field
PHARMACEUTICALS
Status
Email
Parent Application

Applicants

RAJESH KHANNA
A-17 LAWRENCE ROAD NEW DELHI, DELHI-110035 INDIA

Inventors

1. RAJESJ KHANNA
R/O 8650-BHARGHAR, ROSHNARA ROAD, DELHI-07
2. SHIVAM ARORA
R/O D-77, SECTOR-20, NOIDA

Specification

(1) FIELD OF THE INVENTION The present invention generally relates to the field ofa functional carbonated ready-todrink formulation. The invention, particularly relates toa functional carbonated ready-todrink formulation comprising seven ingredients WithaniaSomnifera; BacopaMonneiri; Asphaltum; ChlorophytumBorivilianum; Panax; TribulusTerrestris; and Asparagus Racemosus. (2) BACKGROUND OF THE INVENTION A soft drink is a drink that typically contains carbonated water, a sweetener, and a natural or artificial flavoring. The sweetener may be sugar, high-fructose corn syrup, fruit juice, or some combination of these. Soft drinks may also contain caffeine, colorings, preservatives, and other ingredients. Further, if more herbs are added to the soft drink then is known as "herbal soft drink". Many times, "herbal soft drink" can also refer as "functional beverage". The functional beverage functional beverage is a non-alcoholic drink. Some include ingredients like herbs, vitamins, minerals, amino acids, or additional raw fruit or vegetables. Examples of functional beverages include sports and performance drinks, energy drinks, ready to drink (RTD) teas, enhanced fruit drinks, soy beverages, and enhanced water. Functional beverages have become popular among people who want specific health benefits from their foods and beverages. Both convenience and health have been identified as important factors in consumers' decision-making about food and beverage purchases. Functional drinks are advertised as having various health benefits. For example, some claim to improve heart health, immunity, digestion, and joint health, while others promote themselves as satiating and energy-boosting. A number of different types of herbal or functional formulations are available in the prior art. Prior art document, EP1572221A1 discloses a novel herbal soft drink comprising decoction of plants selected from Sidasps., Vitisvinifera, Withaniasomnifera, Boerhaaviadiffusa and Tinosporacordifolia for the protection and prevention of health and in particular, but not exclusively with antioxidant, immunoenhancing, hepatoprotective, cardiotonic, diuretic, digestive, choleretic, nervine relaxant properties. Another prior art document, JP2013183723A discloses a beverage containing reed leaf powder, ashwagandha leaf powder and rice powder. Yet another prior art document, US5417994 discloses microstaoie son annK oeverages containing at least two eaioie acias cnosen trom phosphoric, citric and malic acids as a microstabilizer, in combination with water, a flavor system of one or more flavor extracts and distillates, and a sweetener, such as HFCS. Yet another prior art document, US20090175964 discloses a composition comprising theanine and a herb selected from Shankhpushpi and/or Shatavari for use as a medicament in the treatment or prevention of a disease associated with reduced immunity in an individual. The disease may, for example, be cold or influenza. Yet another prior art document, KR101367885 discloses a composition comprising the extract of combined herb of Panax ginseng and Vitis genus plant treating and preventing neuro-degenerative disease and enhancing memory power. Another prior art document, US20060062863discloses compositions for anti-obesity, health-restorative and health promotional benefits to mammals, more particularly humans. This composition is obtained from higher plants belonging to genus Chlorophytum. The extraction process for obtaining such composition, as well as pharmaceutical, nutritional and veterinary use products thereof, are also disclosed. Yet another prior art document, WO2015142611 discloses a non-carbonated preoperative beverage containing Panax ginseng. Non-patent literature prior art document discloses a preparation of Green Tea and Brahmi extract powder hard vegetable capsule by manual filling machine.The hard vegetable capsule is used for healthy brain (see: http://iippr.humaniournals.coni/fomiulation-and-evaluation-of-hard-vegetable-capsule-fo healthv-brain-using-powder-of-green-tea-and-brahmi-extract/ ). Yet another prior art document, US20160235801 discloses a method and formulation for fluids, such as drinking water, containing plant phytochemicals, brahmi extract. These phytochemicals have been shown to exhibit antioxidant properties, promote memory and provide additional health benefits, as well as replace bottled water or other fluids as a means to ensure proper hydration. Another prior art document, WO2009054002 discloses a soft drink based on Indian gooseberry extract and a process of production thereof comprising the following steps: - washing and drying of fresh Indian gooseberries, - cutting the berries into small pieces and further drying for a couple of weeks, - extracting the dried berries with water, - filtering, boiling and pasteurizing the extract, - adding sugar or sweeteners and preservatives, - treating with UV-light and filtering, - carbonating, and - packaging of the resulting soft drink. However, above mentioned references and many other similar references have one or more of the following shortcomings: (a) containing only few of the ingredients; (b) noncarbonated drinks/beverages; (cj expensive; (d) having some side ettects; (e) having only fewer or specific health benefits; (f) having higher sugar content; and (g) not in the form of ready-to-drink.There remains a constant need in society for an innovative and novel beverages or drinks containing herbal ingredients. (3) SUMMARY OF THE INVENTION In the view of the foregoing disadvantages inherent in the known types of herbal soft drinknow present in the prior art, the present invention provides a functional carbonated ready-to-drink formulation. As such, the general purpose of the present invention, which will be described subsequently in greater detail, is to provide a new and improved functional carbonated ready-to-drink formulation comprising at least five ingredients selected from the group ofherbs WithaniaSomnifera; BacopaMonneiri; Asphaltum; ChlorophytumBonvilianum; Panax; TribulusTerrestris; and Asparagus Racemosus. An object of the invention is to provide a functional carbonated ready-to-drink formulation comprising at least five ingredients selected from the group of WithaniaSomnifera; BacopaMonneiri; Asphaltum; ChlorophytumBonvilianum; Panax; TribulusTerrestris; and Asparagus Racemosus. It is another object of the present invention to provide the functional carbonated ready-todrink formulation, wherein w/w ratio of WithaniaSomnifera :BacopaMonneiri is 3:2. Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein w/w ratio of Asphaltum :ChlorophytumBorivilianum is 1:3. Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein w/w ratio of Panax : Asparagus Racemosus is 0.5:1. Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein w/w ratio of WithaniaSomnifera .TribulusTerrestris is 2:1. Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein w/w ratio of TribulusTerrestris :BacopaMonneiri is 0.75:1. Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein w/w ratio of Panax : Asparagus Racemosus : TribulusTerrestris is 1:2:2.5. Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, further comprises aloevera, a food-grade gum, and sugar. Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein the food-grade gum is selected from the group consisting of guar gum, acacia gum, xanthan gum, tragacnath, locust bean gum, carrageenan, arabinogalactam, tara, gellan, or mixture thereof. Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein the food-grade gum is selected from the group consisting of xanthan gum, guar gum or mixture thereof. Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein the ready-to-drink formulation has at least 5 degree Brix sugar content. Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein the ready-to-drink formulation having the sugar content in the range of 5 to 25 degree Brix, Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein the ready-to-drink formulation having the sugar content in the range of 9 to 12 degree Brix. It is also object of the present invention to provide a functional carbonated ready-to-drink formulation comprising: WithaniaSomnifera is in range of about 1080 to 1320 mg per liter of said ready-to-drink formulation; BacopaMonneiri is in range of about 720 to 880 mg per liter of said ready-to-drink formulation; Asphaltum is in range of about 252 to 308 mg per liter of said ready-to-drink formulation; ChlorophytumBonvilianum is in range of 5 of 26 • Atty. doc. No. 001 y about 756 to 924 mg per liter of said ready-to-drink formulation; Panax is uiYange of abouf w "^ ""*• 216 to 264 mg per liter of said ready-to-drink formulation; TribulusTerrestris is in range of about 540 to 660 mg per liter of said ready-to-drink formulation; and Asparagus Racemosus is in range of about 432 to 528 mg per liter of said ready-to-drink formulation. 5 Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein WithaniaSomnifera is 1200 mg per liter of said ready-todrink formulation; BacopaMonneiri is 800 mg per liter of said ready-to-drink formulation; Asphaltum is 280 mg per liter of said ready-to-drink formulation; 10 ChlorophytumBorivilianum is 840 mg per liter of said ready-to-drink formulation; Panax is 240 mg per liter of said ready-to-drink formulation; TribulusTerrestris is 600 mg per liter of said ready-to-drink formulation; and Asparagus Racemosus is 480 mg per liter of said ready-to-drink formulation. 15 Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, further comprises aloevera, a food-grade gum, and sugar. Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein the food-grade gum is selected from the group consisting of 20 guar gum, acacia gum, xanthan gum, tragacnath, locust bean gum, carrageenan, arabinogalactam, tara, gellan, or mixture thereof. Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein the food-grade gum is selected from the group consisting of xanthan gum, guar gum or mixture thereof. 25 Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein the ready-to-drink formulation has at least 5 degree Brix sugar content. 30 Yet it is another object of the present invention to provide the functional carbonated readyto- drink formulation, wherein the ready-to-drink formulation having the sugar content in the range of 5 to 25 degree Brix. 6 of 26 • Yet it is another object of the present invention to"pfbvide lhTfun^tTonarc^6bna!ed'rea3y^ to-drink formulation, wherein the ready-to-drink formulation having the sugar content in the range of 9 to 12 degree Brix. In this respect, before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not limited in its application to the details of construction and to the arrangements of the components set forth in the following description or illustrated in the drawings. The invention is capable of other embodiments and of being practiced and carried out in various ways. Also, it is to be understood that the phraseology and terminology employed herein are for the purpose of description and should not be regarded as limiting. These together with other objects of the invention, along with the various features of novelty which characterize the invention, are pointed out with particularity in the disclosure. For a better understanding of the invention, its operating advantages and the specific objects attained by its uses, reference should be had to the accompanying drawings and descriptive matter in which there are illustrated preferred embodiments of the invention. 4) DETAILED DESCRIPTION OF THE INVENTION In the following detailed description, reference is made to the accompanying drawings which form a part hereof, and in which is shown by way of illustration specific embodiments in which the invention may be practiced. These embodiments are described in sufficient detail to enable those skilled in the art to practice the invention, and it is to be understood that the embodiments may be combined, or that other embodiments may be utilized and that structural and logical changes may be made without departing from the spirit and scope of the present invention. The following detailed description is, therefore, not to be taken in a limiting sense, and the scope of the present invention is defined by the appended claims and their equivalents. The description will clearly enable one skilled in the art to make and use the invention, and describes several embodiments, adaptations, variations and alternatives and uses of the invention, including what we presently believe is the best mode for carrying out the invention. It is to be clearly understood that routine variations and adaptations can be made to the invention as described, and such variations and adaptations squarely fall within the spirit and scope of the invention. In other words, the invention is described in connection with such embodiments, but the invention is not limited to any embodiment. The scope of the invention is limited only by the claims and the invention encompasses numerous alternatives, modifications and equivalents. Numerous specific details are set forth in the following description in order to provide a thorough understanding of the invention. These details are provided for the purpose of example and the invention may be practiced according to the claims without some or all of these specific details. For the purpose of clarity, technical material that is known in the technical fields related to the invention has not been described in detail so that the invention is not unnecessarily obscured. The present invention will now be described in brief with reference to the accompanying drawings. Now, refer in more detail to the exemplary drawings for the purposes of illustrating non-limiting embodiments of the present invention. ? As used herein, the term "comprising" and its derivatives including "comprises" and "comprise" include each of the stated integers or elements but does not exclude the inclusion of one or more further integers or elements. • As used herein, the singular forms "a", "an", and "the" include plural referents unless the context clearly dictates otherwise. For example, reference to "a substituent" encompasses a single substituent as well as two or more substituents, and the like. • As used herein, the terms "for example", "for instance", "such as", or "including" are meant to introduce examples that further clarify more general subject matter. Unless otherwise specified, these examples are provided only as an aid for understanding the applications illustrated in the present disclosure, and are not meant to be limiting in any fashion. • As used herein, the term "ready-to-drink formulation" shall refer to "beverage", "drink", "soft drink", "liquid", "cola", "soda", "carbonated drink", "RTD", "Ready-to-take", v "arinjcaDie", "liquid taKen oy mouur, or comoination or mixtures or airrerent Torms ur / objects. • As used herein, the term "carbonated" shall refer to "carbon dioxide purged!', "carbon dioxide included", "carbon dioxide mixed", "carbon dioxide containing", "soda", "C02", or combination or mixture of different terms of object. Carbonated water is a primary ingredient of soft drinks. Carbonated water was created by Joseph Priestly in the year 1767. Carbonated formulation is water into which low levels of pressurized carbon dioxide has been dissolved. Carbonated drink has long been considered a wholesome alternative to regular water. Carbonated drink has that characteristic fizz and slightly sour flavor which is why it is preferred by those who dislike the flat taste of plain still water. And aside from it being a zero-calorie drink, soda water offers a handful of other health benefits as well. Carbonated drink has many benefits for the gastrointestinal tract. Carbonated drink eases stomachaches, quells nausea and has been proven to alleviate constipation. WithaniaSomnifera (Ashwagandha) is to restore strength and vigor to those lacking in dynamism and energy, such as convalescents, Ashwagandha stimulates the immune system and promotes the healthy functioning of organism, like adaptogenic plants. WithaniaSomnifera benefits the entire body and mind, and. its range uses is very broad. It protects the body from numerous diseases, helps to prevent cancer and it also possesses aphoridisiac virtues, acting as a powerful regulator. WithaniaSomnifera is a plant that effectively combats all forms of stress and promotes sleep and quality of sleep. BacopaMonneiri (Brahmi) or extracts thereof have been found to sedate, potentiate barbiturate hypnosis, and improve learning on various tasks; glutamatergic mechanisms may be involved. 16 Ingredients of Brahmi that may be responsible for its cognitive effects include the saponinsbacosides A and B. 18-20 Brahmi extracts have been reported to be non-toxic, non-teratogenic and non-mutagenic in rats and monkeys; single and multiple dosing studies in healthy human volunteers have also elicited no adverse effects. a --ii* T /Yspnanum ^mmjn; nas oeen wiueiy useu as a iraumonai meuicine anu nas many oeneius. ~ 'M ^ Asphaltum has many beneficial properties such as anti-ageing, regenerative properties, energy synthesis, blood purifications, increase in sexual potency, prevents and cures diabetes, solves bone related problems, and spiritual enlightenment. ChlorophytumBorivilianum (SafedMusli), also called White Musli, is a potent aphrodisiac and string adaptogenic herb used in ayurveda and herbal science. It is effective for improving male performance, inducing spermatogenesis and providing strength to the muscles.ChlorophytumBorivilianum is one of important herb in ayurveda, which provides * highly beneficial and advisable for diseases like osteoarthritis, male weakness, reduced vitality, and diabetes in thin or weak people, cancer, muscular weakness, fatigue, oligospermia, bodybuilding etc. Panax (ginseng) includes any one of 11 species of slow-growing perennial plants with fleshy roots, belonging to the genus Panax of the family Araliaceae. Panax ginseng is particularly recommended for those lacking in energy, as it is a very strong and healthy stimulant. Panax ginseng contains active complex carbohydrates called ginsenosides, each with the capacity to stimulate or soothe the nervous system, balance metabolic processes, decrease blood sugar, improve muscle tone and stimulate the endocrine system. It enhances the brain's adrenocorticotropic hormone (ACTH) activity without involving the adrenal glands. TribulusTerrestris(gokhru) is a plant from ayurveda where the root and fruits are used for male virility and general vitality, respectively. The roots enhance libido and sexual wellbeing without affecting testosterone while the fruits appear to be potently protective of organ function. Asparagus racemosus (Shatavari) has been widely used in folk medicine and is today a highly commercially important target species. Shatavari (sometimes spelt as Shatawari), also known as Shatamuli includes the herb Asparagus racemosus. It belongs to the family of Liliaceae. The plant is found throughout central and southern India and in lower Himalayas. The herb contains steroidal saponins. It has been traditionally used for making general tonics and is especially used as a tonic for female reproductive function and to .Atty. doc. No. 001 / • 9 •& / T ^ 7 - f t f i - Q f t V f c ! •-"1 S I *-» increase lactation. Additionally it has been used for providing relief from dysentery, spasms, and also for antioxidant, immune stimulant, and antibacterial benefits. 10 Materials Used The following materials have been used in the preparation of Functional Carbonated Ready-to-drink Formulation. WithaniaSomnifera liquid extract The liquid extracts of WithaniaSomniferaor Ashwagandhawas obtained from the supplier. The WithaniaSomnifera liquid extract was extracted from its root. The WithaniaSomnifera liquid extract is of dark brown color and has passed the standard hysic chemical tests, heavy metals content tests and microbiological tests which are shown in the following table. Sr, No. A 1. 2. 3. 4. 5. 6. 7. B. 8. 9. 10. 11. c 12. 13. 14. 15. 16. 17. Test Physico chemical tests Color Odor Water solubility Propylene glycol stability pH (1% w/v aqueous solution) Specific gravity (20 UC) Dry residue (% - 105 UC) Heavy Metals Lead Arsenic Mercury Cadmium Microbiological Tests Total Aerobic Bacteria Yeast and Moulds Salmonella sp. Escherichia Coli Pseudomonas aeroginosa Staphylococcus aureus Results Dark brown liquid Characteristic Compiles Compiles 5.21 1.036 1.27% Compiles Compiles Compiles Compiles Compiles Compiles Absent Absent Absent Absent Standards Dark brown liquid Characteristic Soluble Soluble 4.0-7.0 1.000-1.050 1 % - 2 % 10 ppm 3 ppm 1 ppm 0.3 ppm NMTlOOOcfu/gm NMTlOOcfu/gm Absent Absent Absent Absent 11 of 26 . Atty. doc. No. 001 / 18. \ a Total Coliforms JTm T fci "1 3 ff^ Compiles i i i • i NMTlOcfu/gm BacopaMonneiriLiquid Extract The liquid extracts of BacopaMonnerior Brahmiwas obtained from the supplier. The BacopaMonneri liquid extract was extracted from its whole plant. The BacopaMonneiri liquid extract is of brown color and has passed the standard hysic chemical tests, heavy metals content tests and microbiological tests which are shown in the following table. Sr. No. A 1. 2. '3. 4. 5. 6. 7. B. 8. 9. 10. 11. C. 12. 13. 14. 15. 16. 17. 18. Test Physico chemical tests Color Odor Water solubility Propylene glycol stability pH (1% w/v aqueous solution) Specific gravity (20 UC) Dry residue (%-105 UC) Heavy Metals Lead Arsenic Mercury Cadmium Microbiological Tests Total Aerobic Bacteria Yeast and Moulds Salmonella sp. Escherichia Coli Pseudomonas aeroginosa Staphylococcus aureus Total Coliforms Results Brown color liquid Characteristic Compiles Compiles 5.5 1.02 3.4% Compiles Compiles Compiles Compiles Compiles Compiles Absent Absent Absent Absent Compiles Standards Brown color liquid Characteristic Soluble Soluble 4.0-7.0 1.000-1.050 1 % - 5 % 10 ppm 3 ppm 1 ppm 0,3 ppm NMT lOOOcfu/gm NMTlOOcfu/gm Absent Absent Absent Absent NMTlOcfu/gm Asphaltum Liquid Extract The liquid extracts of Asphaltumor Shilajitwas obtained from the supplier. The Asphaltum liquid extract was extracted from its stone. The Asphaltum liquid extract is of brown color 12 of 26 ,Atty. doc. No. 001 \ and has passed the standard hysic chemical tests, neavy^mefafs" "cdnfent tetfsJan8* ***•*-*" y microbiological tests which are shown in the following table. Sr. No. A 1. 2. 3. 4. 5. 6. 7. B. 8. 9. 10. 11. C. 12. 13. 14. 15. 16. 17. 18. Test Physico chemical tests Color Odor Water solubility Propylene glycol stability pH (1% w/v aqueous solution) Specific gravity (20 °C) Dry residue (%-105 UC) Heavy Metals Lead Arsenic Mercury Cadmium Microbiological Tests Total Aerobic Bacteria Yeast and Moulds Salmonella sp. Escherichia Coli Pseudomonas aeroginosa Staphylococcus aureus Total Coliforms Results Brown color liquid Characteristic Compiles Compiles 5.68 1.032 1.08% Compiles Compiles Compiles Compiles Compiles Compiles Absent Absent Absent Absent Compiles Standards Brown color liquid Characteristic Soluble Soluble 4.0^7.0 1.000-1.050 0.5% - 1 . 5% 10 ppm 3 ppm 1 ppm 0.3 ppm NMTlOOOcfu/gm NMTlOOcfii/gm Absent Absent Absent Absent NMTlOcfu/gm ChlorophvtumBorivilianum Liquid Extract The liquid extracts of ChlorophytumBorivilianumor Musliwas obtained from the supplier. The ChlorophytumBorivilianumliquid extract was extracted from its root. The ChlorophytumBorivilianum liquid extract is of brown color transparent liquid and has passed the standard hysic chemical tests, heavy metals content tests and microbiological tests which are shown in the following table. Sr. No. Test Results Standards 13 of 26 ,Atty. doc. No. 001 \ A 1. 2. 3. 4. 5. 6. 7. B. 8. 9. 10. 11. C. 12. 13. 14. 15. 16. 17. 18. a Physico chemical tests Color Odor Water solubility Propylene glycol stability pH (1% w/v aqueous solution) Specific gravity (20 °C) Dry residue (% - 105 °C) Heavy Metals Lead Arsenic Mercury Cadmium Microbiological Tests Total Aerobic Bacteria Yeast and Moulds Salmonella sp. Escherichia Coli Pseudomonas aeroginosa Staphylococcus aureus Total Coliforms e - * T *• v r?i .^ - H Brown coloi transparent liquid Characteristic Compiles Compiles 5.14 1.024 0.86 % Compiles Compiles Compiles Compiles Compiles Compiles Absent Absent Absent Absent Compiles f?i - &s fTs 'T fct ™1 53 rfl -i, n . H -B • '• » a « a *. -a Brown coloi transparent liquid Characteristic Soluble Soluble 4.0-7.0 1.000-1.050 0.5%-1.5% lOppm 3 ppm 1 ppm 0.3 ppm NMTlOOOcfu/gm NMTlOOcfu/gm Absent Absent Absent Absent NMTlOcfu/gm rf3^ 'Jt T Panax Liquid Extract The liquid extracts of Panaxor Ginsengwas obtained from the supplier. The Panax Ginsenghquid extract was extracted from its root. The Panax Ginseng liquid extract is of brown color liquid and has passed the standard hysic chemical tests, heavy metals content tests and microbiological tests which are shown in the following table. Sr. No. A 1. 2. 3. Test Physico chemical tests Color Odor Water solubility Results Brown color liquid Characteristic Compiles • Standards Brown color liquid Characteristic Soluble 14 of 26 ,Atty. doc. No. 001 / 4. 5. 6. 7. B. 8. 9. 10. 11. c. 12. 13. 14. 15. 16. 17. 18. Propylene glycol stability pH (1% w/v aqueous solution) Specific gravity (20 UC) Dry residue (%-105 UC) Heavy Metals Lead Arsenic Mercury Cadmium Microbiological Tests • Total Aerobic Bacteria Yeast and Moulds Salmonella sp. Escherichia Coli Pseudomonas aeroginosa Staphylococcus aureus Total Coliforms Compiles 4.68 1.02 3.4% Compiles Compiles Compiles Compiles Compiles Compiles Absent Absent Absent Absent Compiles Soluble 4.0-7.0 1.000-1.050 1 % - 5 % lOppm 3 ppm 1 ppm 03 ppm NMTlOOOcfu/gm NMTlOOcfu/gm Absent Absent Absent Absent NMTlOcfu/gm W >-jt -&. Asparagus Racemosus The liquid extracts of Asparagus Racemosusor Shatavariwas obtained from the supplier. The Asparagus Racemosushquid extract was extracted from its root. The Asparagus Racemosus liquid extract is of brown color liquid and has passed the standard hysic chemical tests, heavy metals content tests and microbiological tests which are shown in the following table. Sr. No. A 1. 2. 3. 4. 5. 6. 7. Test Physico chemical tests Color Odor Water solubility Propylene glycol stability pH (1% w/v aqueous solution) Specific gravity (20 UC) Dry residue (% - 105 UC) Results Brown color liquid Characteristic Compiles Compiles 3.5 1.028 5.60 % Standards Brown color liquid Characteristic Soluble Soluble 3.5-7.0 1.000-1.050 l % - 5 . 6% 15 of 26 . Atty. doc. No. 001 \ B. 8. 9. 10. 11 C. 12, 13 14. 15 16. 17, 18 Heavy Metals Lead Arsenic Mercury Cadmium Microbiological Tests Total Aerobic Bacteria Yeast and Moulds Salmonella sp. Escherichia Coli Pseudomonas aeroginosa Staphylococcus aureus Total Coliforms •9 - e •• •a. n — - # T ft,. 1 Compiles Compiles Compiles Compiles Compiles Compiles Absent Absent Absent Absent Compiles lOppm 3 ppm 1 ppm 0.3 ppm NMTlOOOcfu/gm NMTlOOcfu/gm Absent Absent Absent Absent NMTlOcfu/gm Aloevera The liquid extracts of Aloveralndicaor Aloeverawas obtained from the supplier. The Aloeveraliquid extract was extracted from its lead. The Aloevera liquid extract is of clear transparent color liquid and has passed the standard hysic chemical tests, heavy metals content tests and microbiological tests which are shown in the following table. Sr. No, A 1. 2. 3. 4. 5. 6. 7. B. 8. 9. 10. Test Physico chemical tests Color Odor Water solubility Propylene glycol stability pH (1% w/v aqueous solution) Specific gravity (20 °C) Dry residue (%-105 UC) Heavy Metals Lead Arsenic Mercury Results Clear Colo Transparent liquid Characteristic Compiles Compiles 4.28 1.032 5.60 % Compiles Compiles Compiles Standards Clear Colo] Transparent liquid Characteristic Soluble Soluble 3.0-7.0 1.000-1.050 l % - 5 . 6% 10 ppm 3 ppm 1 ppm 16 of 26 Atty. doc. No. 001 10 15 11. c. 12. 13. 14. 15. 16. 17. 18. Cadmium Microbiological Tests Total Aerobic Bacteria Yeast and Moulds Salmonella sp. Escherichia Coli Pseudomonas aeroginosa Staphylococcus aureus Total Coliforms Compiles Compiles Compiles Absent Absent Absent Absent Compiles 0.3 ppm NMT1000cfii/gm NMTlOOcfWgm Absent Absent Absent Absent NMTlOcfu/gm All materials are stored at cool, dry and dark clean space. Storage temperature not exceeding 25 °C. EXAMPLE: Example 1: 250 ml Functional Carbonated Readv-to-drink Formulation with 10 degree brix with standard herbal ingredients content A preparation method of 250 ml functional carbonated ready-to-drink formulation according to the present invention is disclosed in detailed as below. In a first container 2.25 mg of a mixture of guar gum and xanthan gum (1:1) was taken and then 3.75 ml of water added to the gum mixture. Gum mixturemixed with water was heated around the temperature 70 °C to completely dissolve the gum in water. In a second container a mixture of seven herbal ingredients and other components were taken. The exact quantity of the each ingredient and other component were taken as: WithaniaSomnifera BacopaMonneiri Asphaltum ChlorophytumBorivilianum Panax TribulusTerrestris Asparagus Racemosus Aloevera 300 mg 200 mg 70 mg 210 mg 60 mg 150 mg 120 mg 3 ml 20 The above described compositions were mixed in the second container with around 70 ml of water. In a next step the gum solution and the water containing herbal ingredients were mixed together and make a total solution. This total solution was added to the individual bottles. 17 of 26 Atty. doc. No. 001

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# Name Date
1 201711012355-Form 5-060417.pdf 2017-04-07
2 201711012355-Form 3-060417.pdf 2017-04-07
3 201711012355-Form 2(Title Page)-060417.pdf 2017-04-07
4 201711012355-Form 1-060417.pdf 2017-04-07
5 201711012355-Form 9-010517.pdf 2017-05-02
6 201711012355-Form 18-010517.pdf 2017-05-02
7 201711012355-FER.pdf 2021-10-17

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