Description:TECHNICAL FIELD

[0001] The present invention relates to the hair care compositions. More specifically, the present invention relates to development of an herbal hair coloring composition using the natural dye isolated from Soymida febrifuga bark for imparting natural hair color and hair conditioning to an individual.

BACKGROUND ART

[0002] Hair colorants/ dyes are used globally both in commercial salons and in home. Their use is also increasing rapidly. Hair colorants are cosmetics that have been employed primarily to cover grey hair but additionally to enhance the color of natural hair or to completely alter it.

[0003] Most of the hair dyes sold in market are synthetic chemicals derived from petroleum feedstocks and their production and use is a major environmental and health concern. These hair colors typically contain chemicals that are toxic to the body or are applied to the hair after it has been bleached.

[0004] Yet, while synthetic dye is less expensive for the business, it has a significant negative impact on the environment and human health due to its danger and carcinogenic properties, which are of major concern globally. Chemical hair dyes also require considerable time to apply and have several undesirable side effects, including itchiness, dandruff, irritation, and black patches on the forehead. Besides that, these synthetic hair dyes are potent and result in additional depigmentation and hair dryness.

[0005] People have become far more aware of the nature of products these days, and they tend to favor those derived from natural sources as they are advantageous over those derived from synthetic chemicals. There is a need for a natural hair color that can be applied straight to the hair without bleaching them since hair color is not only a trend but also a necessity.

[0006] Unlike to synthetic dyes, hair colorants made from natural dyes are not only easy to use but also do not have any negative side effects. Products for coloring hair naturally are made with color that derives from organic sources. The dye made from botanical materials is easy to used product that require no professional assistance.

[0007] US6506374B1 disclose a hair coloring composition suitable for application to hair by containing one or more hair substantive direct dyes, in a controlled evaporation rate vehicle, and on an optional polymeric rub off shield, and a controlled viscosity to be reasonably free flowing yet not drip or cause build-up on the hair.

[0008] FR2966728A1 discloses a coloring composition of human keratin fibers such as hair, in the form of foam comprising: (a) one or more oxyethylenated fatty alcohol (s) comprising at least 50 oxyethylenated units; (b) one or more alkaline agent (s) (c) one or more oxidizing agent (s), (d) one or more oxidation dye precursor (s), optionally one or more several surfactants other than oxyethylenated fatty alcohols comprising at least 50 oxyethylenated units. It also relates to a process for treating human keratin fibers from this composition in the form of foam and aerosol or non-aerosol devices for the generation of this foam.

[0009] Several hair colorants are available in market. However, such hair colorants contain chemical additives that not only damage the hair quality but also can create hazardous side effects. Also, these hair colorant on being chemically synthesized leads to several by-product that are harmful for environment.
[0010] In order to overcome the aforementioned drawbacks, there is a need in the art to develop a hair color composition comprising dye isolated from such as Soymida febrifuga as a natural hair colorant as a substitute of chemically synthesized hazardous hair colorants.

OBJECTS OF THE INVENTION

[0011] The principal object of the present invention is to overcome the disadvantages of the prior art.

[0012] An object of the present invention is to develop a composition comprising biodegradable hair colorant made from a material of plant origin.

[0013] Another object of the present invention is to provide a composition as an alternative to the synthetic dyes, thereby limiting exposure of the toxic component in the environment.

[0014] Another object of the present invention is to provide a composition having additives that are safe, cheap, and readily available.

[0015] The foregoing and other objects of the present invention will become readily apparent upon further review of the following detailed description of the embodiments as illustrated in the accompanying drawings.

SUMMARY OF THE INVENTION

[0016] The present invention relates to develop an herbal hair coloring composition that facilitates adequate safety, effectiveness, and ease of application.

[0017] According to an embodiment of the present invention, a herbal hair coloring composition, comprising i) an herbal decoction (10%) of the S. febrifuga dye in the range of 25- 35 %; ii) a thickening agent i.e., xanthan gum in the range of 0.5 – 1.5 %; iii) a humectant i.e. glycerinin the range of 3.0 – 5.0 %; iv) a chelating agent i.e. sodium gluconate in the range of 0.1-1%; v) a preservative i.e. ethylhexylglycerin, phenoxyethanol (Euxyl PE 9010 in the range of 0.1-1%; and vi) distilled water in the range of 60-70%.

[0018] According to another embodiment of the present invention, the method for preparation of the herbal decoction comprises the following steps, i) extracting the bark powder of the S. febrifuga with water at alkaline pH on a water bath for 40-50 minutes to obtain a red-brown extract; ii) filtering through muslin cloth, followed by centrifuged at 3800-4200 rpm for 10-20 minutes at room temperature to obtain a supernatant; iii) lyophilizing supernatant in Allied Frost Lyophilizer for 24-36 hr to obtain dark red-brown dry powder; and iv) dissolving dark red-brown dry powder in water to obtain a herbal decoction.

[0019] According to another embodiment of the present invention, the method for preparation of the herbal hair coloring composition comprises the following step, i) mixing the thickening agent and humectant in a planetary mixer to obtain a mixture solution A; ii) blending the chelating agent and distilled water to obtain a mixture solution B; iii) adding the mixture solution B to mixture solution A, followed by stirring at 90-110 rpm for 25-35 minutes to obtain a homogeneous solution; and iv) incorporating the preservative and herbal decoction to the homogeneous solution, followed by agitation at 70-90 rpm for 5-15 minutes.

[0020] While the invention has been described and shown with reference to the preferred embodiment, it will be apparent that variations might be possible that would fall within the scope of the present invention.

BRIEF DESCRIPTION OF DRAWINGS

[0021] So that the manner in which the above-recited features of the present invention can be understood in detail, a more particular description of the invention, briefly summarized above, may have been referred by embodiments, some of which are illustrated in the appended drawings. It is to be noted, however, that the appended drawings illustrate only typical embodiments of this invention and are therefore not to be considered limiting of its scope, for the invention may admit to other equally effective embodiments.
[0022] These and other features, benefits, and advantages of the present invention will become apparent by reference to the following text figure, with like reference numbers referring to like structures across the views, wherein:
[0023] Figure 1 illustrates a decoction of the lyophilized dye powder in water;
[0024] Figure 2 illustrates a pictorial representation of the prepared hair coloring composition;
[0025] Figure3 illustrates a pictorial representation for color efficacy of different decoctions of the lyophilized dye powder;
[0026] Figure 4 illustrates a pictorial representation of application efficacy of developed hair colorant;
[0027] Figure 5 illustrates a pictorial representation of microscopic evaluation of smoothness of hair fibre before and after application of hair colorant
[0028] Figure 6 illustrates a pictorial representation of effect of shampoo washings after application on dyed hair.
DETAILED DESCRIPTION OF THE INVENTION

[0029] While the present invention is described herein by way of example using embodiments and illustrative drawings, those skilled in the art will recognize that the invention is not limited to the embodiments of drawing or drawings described and are not intended to represent the scale of the various components. Further, some components that may form a part of the invention may not be illustrated in certain figures, for ease of illustration, and such omissions do not limit the embodiments outlined in any way. The drawings and the detailed description thereto are not intended to limit the invention to the particular form disclosed, but on the contrary, the invention is to cover all modifications, equivalents, and alternatives falling within the scope of the present invention as defined by the appended claim.
[0030] As used throughout this description, the word "may" be used in a permissive sense (i.e., meaning having the potential to), rather than the mandatory sense, (i.e. meaning must). Further, the words "a" or "an" mean "at least one” and the word “plurality” means “one or more” unless otherwise mentioned. Furthermore, the terminology and phraseology used herein are solely used for descriptive purposes and should not be construed as limiting in scope. Language such as "including," "comprising," "having," "containing," or "involving," and variations thereof, is intended to be broad and encompass the subject matter listed thereafter, equivalents, and additional subject matter not recited, and is not intended to exclude other additives, components, integers, or steps. Likewise, the term "comprising" is considered synonymous with the terms "including" or "containing" for applicable legal purposes. Any discussion of documents, acts, materials, devices, articles, and the like are included in the specification solely for the purpose of providing a context for the present invention. It is not suggested or represented that any or all these matters form part of the prior art base or were common general knowledge in the field relevant to the present invention.

[0031] In this disclosure, whenever a composition or an element or a group of elements is preceded with the transitional phrase “comprising”, it is understood that we also contemplate the same composition, element, or group of elements with transitional phrases “consisting of”, “consisting”, “selected from the group of consisting of, “including”, or “is” preceding the recitation of the composition, element or group of elements and vice versa.

[0032] The present invention is described hereinafter by various embodiments with reference to the accompanying drawing, wherein reference numerals used in the accompanying drawing correspond to the like elements throughout the description. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiment set forth herein. Rather, the embodiment is provided so that this disclosure will be thorough and complete and will fully convey the scope of the invention to those skilled in the art. In the following detailed description, numeric values and ranges are provided for various aspects of the implementations described. These values and ranges are to be treated as examples only and are not intended to limit the scope of the claims. In addition, several materials are identified as suitable for various facets of the implementations. These materials are to be treated as exemplary and are not intended to limit the scope of the invention.

[0033] The present invention pertains to formulate a hair coloring composition containing a dye isolated from Soymida febrifuga that is better in terms of color, retentive period on hairs, and conditioning of hairs.

[0034] The herbal hair coloring composition, comprises i) an herbal decoction (10%) of the S. febrifuga dye in the range of 25- 35 %; ii) a thickening agent i.e.,xanthan gum in the range of 0.5 – 1.5 %; iii) a humectant i.e. glycerinin the range of 3.0 – 5.0 %; iv) a chelating agent i.e. sodium gluconate in the range of 0.1-1%; v) a preservative i.e. ethylhexylglycerin, phenoxyethanol (Euxyl PE 9010 ) in the range of 0.1-1%; and vi) distilled water in the range of 60-70%.

[0035] According to another embodiment of the present invention, the method for preparation of the herbal decoction comprises the following steps, i) extracting the bark powder of the S. febrifuga with water at alkaline pH on a water bath for 40-50 minutes to obtain a red-brown extract; ii) filtering through muslin cloth, followed by centrifuged at 3800-4200 rpm for 10-20 minutes at room temperature to obtain a supernatant; iii) lyophilizing supernatant in Allied Frost Lyophilizer for 24-36 hr to obtain dark red-brown dry powder; and iv) dissolving dark red-brown dry powder in water to obtain a herbal decoction.

[0036] The method for preparation of the herbal hair coloring composition comprises the following step, i) mixing the thickening agent and humectant in a planetary mixer to obtain a mixture solution A; ii) blending the chelating agent and distilled water to obtain a mixture solution B; iii) adding the mixture solution B to mixture solution A, followed by stirring at 90-110 rpm for 25-35 minutes to obtain a homogeneous solution; and iv) incorporating the preservative and herbal decoction to the homogeneous solution, followed by agitation at 70-90 rpm for 5-15 minutes.

EXAMPLE
Extraction of dye
[0037] The natural dye was extracted from S. febrifuga bark using a statistically optimized method. In brief, d ye was obtained by extracting the bark powder (5-6g) with water (100 mL) alkaline pH (9-11) on a water bath for 40-45 minutes.
[0038] The obtained red-brown aqueous extract was allowed to cool, filtered through muslin cloth, and then centrifuged at 3800-4200 rpm for 10-20 minutes at room temperature. The obtained supernatants were collected and lyophilized in Allied Frost Lyophilizer for 24-36 hr to yield a dark red-brown powder (25 to 30%) as shown in Figure 1.

Preparation of the herbal coloring composition
[0039] Mixing 1g of xanthan gum and4g of glycerin in a planetary mixer to obtain a mixture solution A. In the meanwhile, blending the 0.2g sodium gluconate and 64g distilled water to obtain a mixture solution B. Further, the mixture solution B to mixture solution A is added, followed by stirring at 100 rpm for 30 minutes to obtain a homogeneous solution. Further, the method comprises of incorporating the 0.8g Euxyl PE 9010 and 30g of 10% herbal decoction to the homogeneous solution, followed by agitation at 80 rpm for 10 minutes to obtain the herbal hair coloring composition as referred in Figure 2.

[0040] Furthermore, the obtained lyophilized dye powder was examined to use in final product formulations. The lyophilized dye powders were prepared into of 5, 10, 15, 20 and 25% decoctions in water (Figure 3) to determine the color imparting efficacy on Fischer Saller Scale.

[0041] The lyophilized powder was weighed in desired quantity in water and heated at 60°C for 2hrs. The decoction was then cooled and filtered to obtain the concentrated natural colorant. The decoctions of varied concentrations were then applied on hair swatches and kept for duration of 30-45 mins. The swatches were subjected to 5 minutes of heat application with hair straightening machine after every 10 minutes to enhance the color penetration.
Table-1 Efficacy of different concentrations of decoctions of lyophilized dye powder and their readings of swatches on the Fischer Saller Scale
Decoction (%) Efficacy Ratings
5 Very less efficacy A
10 Less Efficacy VI
15 Less Efficacy VI
20 Reddish brown colour, Less Efficacy VI
25 Reddish brown colour, Less Efficacy VI

[0042] Referring to figure 3 and table 1, no visible difference was observed in the color imparting ability of the decoctions prepared from the dye at concentrations above 10%. Thus, to develop the hair coloring decoction a 10% decoction of the lyophilized dye was used which provides better efficacy on hair. Also, physical parameter such as pH and absorbance of the decoction is studied and observation were noted in table 2.
Table-2 Physical parameters of decoctions made from the dye
Specification Range Test
pH 7.00-7.50 7.39
Absorbance 4.1- 4.3 4.2

Evaluation of developed hair color composition
Stability studies
Freeze Thaw test
[0043] A freeze thaw test was carried out on hair coloring composition. 100 g of the samples was taken and kept at 4°C for 12 hrs then kept at 45°C for 12 hours. Seven cycles of the above were carried out. The result was noted in tabulated form in table 3.
Table 3: Freeze thaw study observation table for hair coloring composition
Parameters Before 7 Cycles After 7 Cycles
Colour Reddish brown Reddish brown
Texture Smooth Smooth
Flow Thick viscous Thick viscous
pH (25) 7.39 7.32
Viscosity (25) 25,000 cps 25,150 cps
Coalescence of emulsion None None
Phase separation None None
Precipitation of ingredients None None

Centrifugation test
[0044] The centrifugation test is carried out to find the effect of gravity on the finished composition. 5 g of composition was kept in a centrifuge and the centrifuge was operated at a speed of 3750 rpm for two hours. After that the hair coloring composition was removed and observed. The observations were noted in tabulated form in table 4.
Table 4: Effect of centrifugation on hair color decoction
Parameters Before After
Color Reddish brown Reddish brown
Fragrance Smooth Smooth
Texture Thick viscous Thick viscous
pH (25) 7.39 7.32
Viscosity (25) 25,000 cps 25,100 cps
Coalescence of emulsion None None
Phase separation None None
Precipitation of ingredients None None

Accelerated Stability Study
[0045] The hair coloring composition was subjected to different temperatures in refrigerator 4+°C, in stability testing chamber 45°C at 70% relative humidity and Room Temperature for the period of three months. The samples were evaluated for following parameters. The results for the following parameter are represented in tabulated form in table 5, 6, 7.
Determination of pH: 5 g of hair coloring composition was added to 45 ml of water and mixed thoroughly. The solutions were then tested for pH using pH meter.
Determination of Viscosity: The viscosity of hair coloring composition was determined in centipoise by using Brookfield’s Viscometer at 25°C, using spindle 64 at 6rpm.
Determination of organoleptic properties: The hair coloring composition was tested for color, odor, and texture.
Determination of separation of ingredients: The hair coloring composition was also evaluated for separation of ingredients in the form of precipitation and phase separation.
Table 5: Stability of the hair coloring composition at 4ºC
Parameters Day 1 Day 3 Day 7 Day 14 Day 21 Day 28 Day 35 Day 42 Day 49 Day 64 Day
79 Day
94
Color RB RB RB RB RB RB RB RB RB RB RB RB
Texture Soft Soft Soft Soft soft Soft soft Soft Soft soft soft soft
Flow Thick Thick Thick Thick Thick Thick Thick Thick Thick Thick Thick Thick
pH (25) 7.39 7.4 7.47 7.4 7.4 7.5 7.38 7.5 7.38 7.6 7.42 7.37
Viscosity (25) cps 25
000 26,
000 25,
900 26,
100 26,
200 26,
000 26,
100 25,
900 26,
100 26,
300 26,
000 26,
100
Coalescence of emulsion No No No No No No No No No No No No
Phase separation No No No No No No No No No No No No
Precipitation of ingredients No No No No No No No No No No No No

Table 6: Stability of hair coloring composition at Ambient Temperature
Parameters Day 1 Day 3 Day 7 Day 14 Day 21 Day 28 Day 35 Day 42 Day 49 Day 64 Day
79 Day
94
Color RB RB RB RB RB RB RB RB RB RB RB RB
Fragrance Soft soft Soft Soft soft Soft soft Soft Soft Soft soft soft
Texture Soft soft Soft Soft soft Soft soft Soft Soft Soft soft soft
Flow Thick Thick Thick Thick Thick Thick Thick Thick Thick Thick Thick Thick
pH (25) 7.39 7.35 7.37 7.4 7.41 7.37 7.38 7.43 7.32 7.5 7.39 7.4
Viscosity (25) 25
000 25,
800 26,
100 25,
500 26,
100 26,
100 25,
900 25,
800 25,
900 26,
000 26,
100 26,
000
Coalescence of emulsion No No No No No No No No No No No No
Phase separation No No No No No No No No No No No No
Precipitation of ingredients No No No No No No No No No No No No

Table 7: Stability of hair coloring composition at 45 °C and 70% Relative humidity
Parameters Day 1 Day 3 Day 7 Day 14 Day 21 Day 28 Day 35 Day 42 Day 49 Day 64 Day
79 Day
94
Color RB RB RB RB RB RB RB RB RB RB RB RB
Fragrance Soft soft Soft Soft soft Soft soft Soft Soft Soft soft soft
Texture Soft soft Soft Soft soft Soft soft Soft Soft Soft soft soft
Flow Thick Thick Thick Thick Thick Thick Thick Thick Thick Thick Thick Thick
pH (25) 7.39 7.35 7.37 7.4 7.41 7.37 7.38 7.43 7.32 7.5 7.39 7.4
Viscosity (25) 25
000 25,
800 26,
100 25,
500 26,
100 26,
100 25,
900 25,
800 25,
900 26,
000 26,
100 26,
000
Coalescence of emulsion No No No No No No No No No No No No
Phase separation No No No No No No No No No No No No
Precipitation of ingredients No No No No No No No No No No No No

[0046] The hair coloring composition was found to be stable at the end of the accelerated stability study. Hence it can be said that the hair coloring composition has a shelf life of 2 years.
Evaluation of application efficacy of developed hair coloring composition
[0047] About 2g of hair coloring composition was used per hair swatch of 15 cm length and weight of 2 g. The gel was thoroughly and evenly applied on the dry hair swatch. The swatch was wrapped in the foil and was subjected to periodic heat treatments to further aid the percolation of the color within the hair strands. After 45 minutes, the swatch was unwrapped from the foil, was rinsed in with water and washed with an appropriate shampoo having a pH between 4.5-5.0. The swatches were dried using a hair dryer. The readings of the swatches were further evaluated on the Fischer Saller Scale is noted in tabulated form in table 8 and observed in Figure 4.
Table 8: Reading of the swatches
Hair Swatches Ratings
A A
B VII
Evaluation of hair coloring composition on hair quality
[0048] The hair coloring composition was further compared for conditioning efficacy by comparing with a marketed hair dye. Both the hair coloring compositions were evaluated with a view to evaluate the effect of extract on hair. A comparative analysis of the hair coloring compositions was done on basis of its efficacy parameters.

[0049] Efficacy parameters- Both the hair coloring composition were studied for the activity aspects by analyzing the combing efficacy and by microscopical studies. All the studies carried pointed out that the hair coloring composition containing extract not only help in better coloring of hair but also help in conditioning of hair. It was found to be at par with marketed hair coloring composition.

[0050] Two hair swatches of 15 cm length and 5g weight from same origin were taken. The swatches where first wetted with water. Then 2 gm of developed hair coloring composition D and marketed product DA (Godrej hair dye) were applied on the hair swatches and kept for a period of 30 minutes.

[0051] These were then washed off with running water and tested for dry combing and physical appearance. The swatches were subjected to the evaluation of following criteria’s Ease of combing: wet combing, dry combing, shine, flyaway and feel. Ease of combing is done on both wet and dry hair.

[0052] The force required to comb the hair swatches is compared for all the samples. Shine was evaluated by visual evaluation by a panel of 16 people. The swatches were attached at the root end and mounted on a cylinder. Sources of four bulbs were kept in a row 10 inches above the tresses and the luster was evaluated. Flyaway of the swatches was evaluated by visual method. The subjects were required to fill up a questionnaire after hair coloring composition evaluation.

[0053] Subjects filled up questionnaire with ratings hair on following attributes: wet combing, dry combing, flyaway, shine, overall feel of hair, tangling of hair. The observations were noted in Figure 5 and Table 9 and Table 10.
Table 9: Effect of developed hair colouring composition D on conditioning of hair (Herein, M stands for male and F stands for female)
volt No. M/F Wet comb Dry Comb Fly away Shine Feel Detangling
1 M 4 3 3 2 1 2
2 F 3 3 3 2 2 1
3 F 3 3 3 2 2 1
5 M 4 2 3 2 2 1
6 F 4 3 3 2 2 1
7 F 4 3 3 2 2 2
8 M 4 3 3 1 2 2
10 F 4 4 3 2 2 2
11 F 3 3 2 1 2 1
12 F 3 3 2 2 2 1
13 M 3 3 2 2 2 1
14 F 4 3 3 2 2 1
15 M 4 2 3 2 2 1
16 M 4 3 3 2 3 2
average 3.642857 2.928571 2.785714 1.857143 2 1.357143
std devi ± 0.497245 0.474631 0.425815 0.363137 0.392232 0.497245
Table 10: Effect of marketed dye DA on conditioning of hair
volt No. m/f Wet comb Dry Comb Fly away Shine Feel Detangling
1 M 2 2 2 1 2 2
2 F 2 2 3 2 1 2
3 F 2 2 3 2 3 2
5 M 3 2 3 1 2 1
6 F 2 2 2 2 2 1
7 F 2 2 2 3 2 1
8 M 4 2 4 2 1 1
10 F 3 2 3 3 1 1
11 F 2 2 3 2 1 1
12 F 2 2 3 2 1 4
13 M 2 2 3 2 1 1
14 F 3 3 2 2 1 1
15 M 3 3 2 2 1 1
16 M 2 2 2 2 1 1
Average 2.428571 2.142857 2.642857 2 1.428571 1.428571
std devi ± 0.646206 0.363137 0.633324 0.5547 0.646206 0.851631
All attributes mentioned above were assessed using the 5-point scale:
5=Excellent; 4=Very Good; 3= Good; 2= Average; 1= Poor.
In case of tangling the ratings were as below
1=Excellent; 2=Very Good; 3= Good; 4= Average; 5= Poor

Evaluation of retentivity of color on hair
[0054] To observe the longevity of color on hair the following test was performed. The hair swatches were first wetted with water and 2g of developed hair color gel was applied using thumb and index finger in top to bottom direction with swapping of sides after each spreading and left for 30 minutes.

[0055] The swatches were then rinsed off with running water. The swatches were then combed and dried with a hair drier. Next day the swatches were given 7 consecutive washes with Sun silk shampoo. The individual hair from swatches was then observed for color under optical microscope. The observations were noted in Figure 6.

[0056] Various modifications to these embodiments are apparent to those skilled in the art from the description and the accompanying drawings. The principles associated with the various embodiments described herein may be applied to other embodiments. Therefore, the description is not intended to be limited to the 5 embodiments shown along with the accompanying drawings but is to be providing the broadest scope consistent with the principles and the novel and inventive features disclosed or suggested herein. Accordingly, the invention is anticipated to hold on to all other such alternatives, modifications, and variations that fall within the scope of the present invention and appended claims.
, Claims:I/We Claim:
1) An herbal hair coloring composition, comprising:
i) an herbal decoction (10%) in the range of 25- 35 %;
ii) a thickening agent in the range of 0.5 – 1.5 %;
iii) a humectant in the range of 3.0 – 5.0 %;
iv) a chelating agent in the range of 0.1-1%;
v) a preservative in the range of 0.1-1%; and
vi) distilled water in the range of 60-70%.

2) The composition as claimed in claim 1, wherein the herbal decoction is the solution of S. febrifuga bark dye in water .
3) The composition as claimed in claim 2, wherein the method for preparation of the herbal decoction comprises the following steps,
vii) extracting the bark powder of the S. febrifuga with water at alkaline pH on a water bath for 40-50 minutes to obtain a red-brown extract
viii) filtering through muslin cloth, followed by centrifuged at 3800-4200 rpm for 10-20 minutes at room temperature to obtain a supernatant;
ix) lyophilizing supernatant in Allied Frost Lyophilizer for 24-36 hr to obtain dark red-brown dry powder; and
x) dissolving dark red-brown dry powder in water to obtain the herbal decoction.

4) The composition as claimed in claim 1, wherein the thickening agent is xanthan gum.

5) The composition as claimed in claim 1, wherein the humectant is glycerin.

6) The composition as claimed in claim 1, wherein the chelating agent is sodium gluconate.

7) The composition as claimed in claim 1, wherein the preservative is ethylhexylglycerin, phenoxyethanol (Euxyl PE 9010).

8) A method for preparation of the herbal hair coloring composition comprises the following step,
i) mixing the thickening agent and humectant in a planetary mixer to obtain a mixture solution A;
ii) blending the chelating agent and distilled water to obtain a mixture solution B;
iii) adding the mixture solution B to mixture solution A, followed by stirring for 25-35 minutes to obtain a homogeneous solution; and
iv) incorporating the preservative and herbal decoction to the homogeneous solution, followed by agitation at 70-90 rpm for 5-15 minutes to obtain hair coloring composition.
9) The method as claimed in claim 8, wherein the stirring is performed at 90-110 rpm.