FIELD OF THE INVENTION:
The present invention relates to combination of herbicides for controlling undesirable plants. The present invention more specifically relates to a synergistic combination of herbicides.
BACKGROUND OF THE INVENTION:
Weeds are undesirable plants that can severely damage yield in crops. Farmers usually control these plants at the pre-plant stage as well as after sowing. Modern herbicides are used to either control or suppress these undesirable plants so as to allow sown crops a greater share of nutrient.
Chemical herbicides have provided an effective way of controlling weeds over the years. Chemical herbicides can generally be applied pre-planting, pre-emergence and/or post emergence. The use of chemical herbicides is sometimes the only practical and selective process of managing certain weeds. Herbicides control weeds by either stopping or changing the weed's normal growth patterns. It has been observed that weeds are capable of developing resistance to herbicides through genetic mutation and soon become tolerant to that particular herbicide. One of the frequently adopted practices to control tolerance is resistance management where two or more herbicide active substances with different mechanisms of actions are combined together and contribute the desired properties to control weed growth. The use of mixtures or sequences of herbicides with differing mechanism of actions are important specially to prevent or overcome resistance caused by single herbicides.2019030091A2
Combinations of herbicides are used to control a broader range of weeds. However, the combination of herbicides may not always result in the desired effect. Combination of herbicides may lead to an additive effect or an antagonistic effect. It may also result in phytotoxicity to the crops making it an undesirable combination. Agronomists must therefore, carefully select the

herbicides that can be combined to offer a synergistic effect that would control weeds while having no phytotoxic effect on the crop and reduce the chances of development of herbicide resistant weeds.
There is therefore a need in the art for combinations that have advantageous properties such as a herbicidal combination that is synergistic, helps in resistance management, reduces dosage of herbicides used thus causing minimal damage to the environment, a herbicidal combination that has excellent residual effects. The present invention aims at providing such a herbicidal combination for particular crops such as onion and garlic crop.
OBJECTS OF THE INVENTION:
It is an object of the present invention to provide a herbicidal composition comprising Quizalofop ethyl (l-15%w/w), Oxyfluorfen (5-35%w/w) and Vegetable oil (l-10%w/w) which is free from the existing drawbacks of the herbicidal compositions.
Yet another objective of the present invention is to provide a chemically stable synergistic herbicidal composition, which is effective in controlling both narrow as well as broad leaved weeds in agricultural crops particularly on onion crop.
Still another objective of the present invention is to provide a chemically stable synergistic herbicidal composition, which demonstrates a high controlling effect with reduced crop protection cost, increased crop yield and reduced environmental load.
Yet another objective of the present invention is to provide a chemically stable synergistic composition which has a broader spectrum of activity and reduces the risk of developing resistance.
SUMMARY OF THE INVENTION:

The present invention relates to a novel, synergistic herbicidal composition for control of narrow-leaved and broad-leaved weeds in agricultural crops, particularly onion (Allium cepa)
Specifically, it pertains to a herbicidal composition comprising Quizalofop ethyl, Oxyfluorfen and vegetable oil as active ingredients in specific weight percentages along with at least one agriculturally acceptable excipient. The present invention further relates to a process for preparation of said novel, synergistic herbicidal composition.
An aspect of the present invention relates to a novel, synergistic herbicidal composition comprising a combination of a) Quizalofop ethyl in an amount ranging from 1-15% w/w of the herbicidal composition b) Oxyfluorfen in an amount ranging from 5-35% w/w of the herbicidal composition c) Vegetable oil in an amount ranging from 1-10% w/w of the herbicidal composition d) at least one agriculturally acceptable excipient.
Another aspect of the present invention relates to a process for preparation of said novel, synergistic herbicidal composition comprising the specified steps.
Yet another aspect of the present invention relates to the novel, synergistic herbicidal composition, wherein said herbicidal composition is in the form of solid or liquid.
DETAILED DESCRIPTION OF THE INVENTION
Definitions:
The following terms have the indicated meanings when used herein:
As used herein, a herbicidally effective amount is an amount of active ingredient which causes an adversely modifying effect in targeted weeds. Such effects

include deviations from natural development, killing, regulation, desiccation, retardation, and the like.
As used herein, post-emergence control of weeds means preventing, reducing, killing, or otherwise adversely modifying the development of the weeds with negligent adverse effect on the crop plants
As used herein, the "undesirable plants" are, for example but not limited to, harmful grasses or weeds, leafy plants, and gramineous plants.
As used herein, the term "weed" refers to the unwanted plant which competes for resources with the crop or plant of interest.
As used herein, the term "herbicide" refers to a substance which adversely affects the existence and growth of the target weed.
As used herein the term "effective amount" is that quantity of active agent, applied in an amount which will provide the required control of weed on commercial crops. The amount is dependent upon many factors including, for example, type of formulations, the crop, weed sought to be controlled and environmental conditions.
As used herein, the term "agrochemical excipient" refers to an ingredient, additive, component or supplement, liquid or solid, suitable for incorporation in agricultural compositions.
As used herein, the terms "formulation" and "composition" may be used interchangeably and refer to a mixture of two or more compounds, elements, molecules, etc. In some aspects the terms "formulation" and "composition" may be used to refer to a mixture of one or more active ingredients with a carrier or other excipients.

As used herein, the terms "comprise", "comprising", "include", "includes", and "including" are meant to be non-limiting i.e. other steps and other ingredients which do not affect the end of result can be added. The above terms encompass the terms "consisting of" and "consisting essentially of".
Quizalofop ethyl is a selective systemic herbicide of Aryloxyphenoxy-propionates group. It is a post emergent herbicide for the control of grassy weeds in broad leaved and some other crops. It is a good systemic herbicide, absorbed from the leaf surface, with translocation throughout the plant, moving in both the xylem and phloem. It is very quickly absorbed by the weeds and translocated and kill the weeds. The affected weeds are unable to regenerate. It is recommended for the control of weeds in soybean, cotton, groundnut, black gram and onion.
Oxyfluorfen is a herbicide of the diphenyl-ether group used for selective weed control in a wide range of fruit trees, vegetables, field crops, ornamentals, forestry, sugarcane and non-crop areas. It controls a wide spectrum of annual broad-leaved and grass weeds. It is used in pre-plant, pre-emergence and post-emergence applications in bulb crops like onion field crops. It has prolonged residual activity and negligible leaching.
Vegetable oils are oils extracted from seeds, or less often, from other parts of fruits. Like animal fats, vegetable fats are mixtures of triglycerides. Soybean oil, rapeseed oil, sunflower oil, linseed oil, cocoa butter and other essential and aromatic oil from medicinal plants . Olive oil, palm oil, and rice bran oil are examples of fats from other parts of fruits. In common usage, vegetable oil may refer exclusively to vegetable fats which are liquid at room temperature.
The present invention relates to a novel, synergistic broad-spectrum herbicidal composition for control of narrow leaved weeds and broad-leaved weeds in Onion crop (Allium cepa). Specifically it pertains to synergistic herbicidal composition comprising Quizalofop ethyl, Oxyfluorfen and Vegetable oil in specific weight percentages along with at least one agriculturally acceptable

excipient for control of narrow leaved weeds and broad-leaved weeds in Onion (Allium cepa) crop. The invention further relates to a process for preparing the composition thereof.
Surprisingly it was observed that the herbicidal composition of the present invention provides a superior weed control at much lower levels of active ingredient combination as compared to what is achieved when the active ingredients are applied alone. Accordingly, in an embodiment the present invention relates to a synergistic herbicidal composition comprising 1) Quizalofop ethyl in an amount ranging from 1-15% w/w of the herbicidal composition 2) Oxyfluorfen in an amount ranging from 5-35% w/w of the herbicidal composition 3) Vegetable oil in an amount ranging from 1-10% w/w w/w of the herbicidal composition 4) at least one agriculturally acceptable excipient.
In an embodiment, the herbicidal composition further includes at least one agriculturally acceptable excipient selected from the group comprising a wetting agent, dispersing agent, a binder, carrier, antifoaming agent, etc.
In an embodiment, the herbicidal composition of the present invention may be formulated in a form selected from the group comprising water-soluble concentrates (SL), emulsifiable concentrates (EC), emulsions (EW), micro-emulsions (ME), Suspension concentrates (SC), oil-based suspension concentrates (OD), flowable suspensions (FS), water-dispersible granules (WDG), water-soluble granules (SG), wettable powders (WP), water soluble powders (SP), granules (GR), encapsulated granules (CG), fine granules (FG), macrogranules (GG), aqueous Suspo-emulsions (SE), capsule suspensions (CS) and microgranules (MG). Preferred formulations are suspension concentrates (SC), wettable granules (WG) and wettable powders (WP).
In an embodiment the herbicidal composition of the present invention is formulated in a form of Wettable granule (WG). In an embodiment, the water

dispersible granules of the present invention comprise a wetting agent, a dispersing agent, a defoaming agent, a binder and a suitable carrier.
In an embodiment the present composition comprises one or more dispersing agents, wherein said dispersing agent is selected from the group comprising sodium lignosulphonates, sodium naphthalene sulphonate- formaldehyde condensates, aliphatic alcohol ethoxylates, tristyrylphenol ethoxylates and esters, ethylene oxide/propylene oxide block copolymers. Preferably the dispersing agent is sodium salt of naphthalene sulfonate condensate
In an embodiment said dispersing agent may be present in an amount ranging from 6-10% w/w of the herbicidal composition.
In an embodiment, the present composition comprises one or more wetting agents, wherein said wetting agent is selected from the group comprising blend of alkyl naphthalene sulfonate, sodium salt, Sodium laurel sulphate. In a preferred embodiment, the wetting agent is Sodium laurel sulphate.
In an embodiment, the wetting agent may be present in an amount ranging from 4-8 w/w of the herbicidal composition.
In an embodiment, the present composition comprises one or more anti foaming agents, wherein said anti- foaming agent is selected from the group comprising silicone emulsions, long-chain alcohols, fatty acids, organic fluorine compounds
In an embodiment, the wetting agent may be present in an amount ranging from 0.2 to 0.5 w/w of the herbicidal composition.
In an embodiment, the present composition comprises one or more carriers, wherein said carrier is selected from the group comprising Dextrose, Lactose, Soluble starch, Galactose, Amylodextrin, Maltose, Mannitol, Sucrose, Sorbitol and China clay.

In an embodiment, the carrier may be present in an amount ranging from 5-10%w/w of the herbicidal composition.
In an embodiment, the present composition comprises one or more binders, wherein said binder is selected from the group comprising Starch paste, Hydroxypropyl methylcellulose (HPMC), Polyvinyl Pyrrolidone (PVP), Lactose monohydrate. In a preferred embodiment, said binder is lactose monohydrate.
In an embodiment, the present herbicidal composition comprises Quizalofop ethyl in an amount ranging from 1-15% w/w of the herbicidal composition 2) Oxyfluorfen in an amount ranging from 5-35% w/w of the herbicidal composition 3) Vegetable oil in an amount ranging from 1-10% w/w w/w of the herbicidal composition.
In an embodiment, the present herbicidal composition comprises Quizalofop ethyl in an amount of 8.8% w/w of the herbicidal composition 2) Oxyfluorfen in an amount of 19% w/w of the herbicidal composition 3) Vegetable oil in an amount of 1% w/w of the herbicidal composition.
In an embodiment, the present herbicidal composition comprising Quizalofop ethyl (8.8%), Oxyfluorfen (19%) and vegetable oil (1%) at a dosage of 600ml/hectare shows a 95% inhibition in the weed density of Eleucine indica (narrow leaved weed).
In an embodiment, the present herbicidal composition comprising Quizalofop ethyl (8.8%), Oxyfluorfen (19%) and vegetable oil (1%) at a dosage of 600ml/hectare shows a 100% inhibition in the weed density of Portulaca spp. (Broad leaved weed)
In an embodiment, the present herbicidal composition comprising Quizalofop ethyl (8.8%), Oxyfluorfen (19%) and vegetable oil (1%) at a dosage of

600ml/hectare shows a 100% inhibition in the weed density of Lactuca spp. (Broad leaved weed)
In an embodiment, the present herbicidal composition is effective in controlling narrow leaved weeds including but not limited to Echinochloa spp., Eleucine indica, Bracharia spp. and broad leaved weeds including but not limited to Portulaca spp., Parthenium spp. Amaranthus spp., Lactuca spp., Trianthema spp. and Alternanthera spp.
EXAMPLES:
The examples below are given solely for the purpose of illustration and are not to be construed as limitations of the present invention, as many variations thereof are possible without departing from the spirit and scope of the invention.
EXAMPLE 1: Preparation of combination of the present invention:
In an embodiment, the preparation of the present invention was carried out by undertaking following steps:
EXAMPLE 1: Preparation of combination of the present invention:
The preparation of the present invention was carried out by undertaking following steps:
STEP 1: The raw material such as wetting agent, dispersing agent, filler and Quizalofop ethyl technical were added and mixed to result in a homogeneous mixture. This was followed by addition of oxyfluorfen technical and vegetable oil in the given proportion.
STEP 2: The above mixed mass was blended and grinded in a jet mill. Grinding was carried out until a mean particle size of below 10 micron was obtained.

STEP 3: After grinding, the mixture was again blended in blender (Post mixing blending) to make the mixture homogenous.
STEP 4: The dough was prepared by dough mixer and granules were prepared though an extruder
STEP 5: The granules obtained in above step were dried in hot air or Oven.
STEP 6: Finally, quality parameters were checked.
Table 1: Chemical combination (in percentage)

S. No. Chemical constituent %combination (w/w)
1 Quizalofop ethyl technical (Basis of 100.0%) 8.8% w/w
2 Oxyfluorfen technical (Basis of 100.0%) 19% w/w
3 Vegetable oil 1.00% w/w
4 Sodium bicarbonate 01 to 05 w/w
5 Lactose IP 05 to 10 w/w
6 Dispersing agent (Sodium salt of naphthalene sulfonate condensate) 06 to 10 w/w
7 Wetting agent (sodium laurel sulphate) 04 to 08 w/w
8 Antifoam 0.2 to 0.5 w/w
9 PPT silica 02 to 06 w/w

S. No. Chemical constituent %combination (w/w)
10 China clay QS to make %w/w
Total quantity 100 % w/w
Table 2: Quality parameter of the formulation

S.no. Quality Parameter Desired quality
1 Appearance Material should be free visible extraneous matter, dry and free flowing granules
2 Persistent of foam Persistent of foam should not more than 60 ml after 1 min
3 Wettability Wettability should be max 120 sec
4 Suspensibility Suspensibility should be Max 120 sec
5 Acidity as H2SO4 Acidity should by 0.5% max
EXAMPLE 2: Evaluation of bio efficacy of the present composition
The presently disclosed herbicidal trimix was tested for its bio efficacy against narrow leaved weeds and broad-leaved weeds on Onion crop during Rabi season 2019. The trial was conducted on a local variety at Nashik, Maharashtra India. The plants were aligned in a plot size of 30 sq. m., having a spacing of 15 cm between the individual crop plants and a spacing of 20 cm between the rows. The experiment was laid out in a random block design consisting of a total of 11

treatments in three replications. Further, the trial was conducted at a temperature of 16°C at a relative humidity of 65% and no winds and with optimum soil moisture. The application of the herbicidal trimix was carried out at the early post-emergence stage around 15-29 days after planting of the plants at 2-4 leaves stage of the weeds.
Measured quantity of the formulation was added to the required volume of water @ 375lit./Ha for spray application. The spray tank was filled with half the quantity of clean required volume of water and then the measured chemical (according to the dose) was added followed by the rest half quantity of water. The solution was stirred well before the application. The knapsack sprayer fitted with boom along with flood jet nozzle was used to apply the herbicidal composition.
TREATMENT DETAILS:
Table 1 below provides the treatment details of the Onion crop under present experiment. For purposes of data presentation, the present composition has been coded as CCP- 8175 WG. It is further stated that the dosages in T2-T3 are based on commercially recommended dosages for effective control of unwanted weeds.
Table 3: Treatment details:

Trial no. Treatment details Dose a.i./hectare Dose/hectare
T-1 Untreated -
T-2 Quizalofop ethyl 5 % EC 50 1000 ml
T-3 Oxyfluorfen 23.5% EC 199.8 850 ml

T-4 CCP-8175 WG (Formulation-1) 40+87.5 500 ml
T-5 CCP-8175 WG (Formulation-1) 48 + 105 600 ml
T-6 CCP-8175 WG (Formulation-1) 56+122.5 700 ml
T-7 CCP-8175 WG (Formulation-2) 40+87.5+5 500 ml
T-8 CCP-8175 WG (Formulation-2) 48 + 105+6 600 ml
T-9 CCP-8175 WG (Formulation-2) 56+122.5+7 700 ml
T-10 CCP-8175 WG (Formulation-3) (52.8 + 114 ) + 6 600 ml
T-ll CCP-8175 WG (Formulation-3) (55+ 119)+ 6.25 625 ml
T-12 CCP-8175 WG (Formulation-3) 57.2+ 124)+ 6.5 650 ml
Note
Formulation -1 CCP-8175 WG Quizalofop 8% + Oxyfluorfen 17.5%
Formulation -2 CCP-8175 WG Quizalofop 8% Oxyfluorfen 17.5% + Vegetable oil
1%
Formulation-3 CCP-8175 WG (Quizalofop 8.8% + Oxyfluorfen 19%) + Vegetable
oil 1%
EVALUATION OF BIO EFFICACY IN ONION CROP
Weed count: A quadrate (0.5m x 0.5m) was placed at 3 randomly selected places in all the plots of the experimental field and the number of weed flora were counted uniformly at 10, 25 and 50 days after application.

Weed control: The weed control was calculated based on the no. of live weed flora at 10, 25, 50 days after application.
Percent weed control = WC - WT
x 100
WC Where WC= No. of weed in control plot
WT= No. of weed in treated plot
Table 3 (a): Effect of herbicidal treatment on weed density of Narrow leaves weeds at 21 days after application

;atments Dose (a.i/ha) Percent control of Narrow weeds (Mean 0.25 sqm) over UTC

Echinochloa spp Eleucine indica Bracharia spp
Live population % control Live population %control Live population %control
treated 13 - 17.5 12 -
izalofop lyl 5% EC 50 3 76.92 2.5 85.71 1.5 87.5
yfluorfen .5% EC 199.8 11 15.38 14 20.0 9.5 20.83
P-8175 )rmulation- 40+87.5 6 53.84 5.5 68.57 4.0 66.66

;atments Dose (a.i/ha) Percent control of Narrow weeds (Mean 0.25 sqm) over UTC

Echinochloa spp Eleucine indica Bracharia spp
Live population % control Live population %control Live population %control
P-8175 )rmulation- 48+ 105 2.5 80.76 3.5 80.00 2.5 79.16
P-8175 )rmulation- 56+122.5 2.25 82.69 3.0 82.85 2.0 83.33
P-8175 )rmulation- 40+87.5+5 2.5 80.76 2.0 88.57 3.0 75.00
P-8175 )rmulation- 48+105+6 1.0 92.30 0.0 100 0.0 100
P-8175 )rmulation- 56+122.5+7 1.0 92.30 0.0 100 0.0 100
P-8175 )rmulation- (52.8 + 114 ) + 6 1.0 90.0 2.5 86.84 3.0 72.72

Treatments Dose (a.i/ha) Percent control of Narrow weeds (Mean 0.25 sqm) over UTC

Echinochloa spp Eleucine indica Bracharia spp
Live population % control Live population %control Live population %control
CCP-8175 WG
(Formulation-3) 55 + 119) + 6.25 1.0 90.0 1.0 94.73 1.0 90.90
CCP-8175 WG
(Formulation-3) 57.2 + 124) + 6.5 0.0 100.0 1.0 94.73 0.0 100.00
Formulation-1 CCP-8175 WG Quizalofop 8% + Oxyfluorfen 17.5%
Formulation-2 CCP-8175 WG Quizalofop 8% + Oxyfluorfen 17.5% + Vegetable oil 1%
Formulation-3 CCP-8175 WG Quizalofop 8.8% + Oxyfluorfen 19% + Vegetable oil 1%
Table 3 (b): Effect of herbicidal treatment on weed density of Broad leaved weeds at 21 days after application

Treatments Dose (a.i/ha) Percent control of broad leave weeds (Mean 0.25

sqm) over UTC

Portulaca spp Parthenium spp Amaranthus spp.

Live popu latio n % control Live
populati
on % control Live
populatio
n % control
Untreated 15.5 - 7.5 10 -
Quizalofop ethyl 5% EC 50 15.5 9.67 6.0 20.00 8.5 15.00
Oxyfluorfen 23.5% EC 199.8 14 90.32 1.0 86.00 2.0 80.00
CCP-8175 WG (Formulation-1) 40+87.5 1.5 80.64 2.5 66.00 3.5 65.00
CCP-8175 WG (Formulation-1) 48+ 105 3.0 83.87 2.0 73.00 3.0 70.00
CCP-8175 WG (Formulation-1) 56+122.5 2.5 93.5 2.0 73.00 2.0 80.00
CCP-8175 WG (Formulation-2) 40+87.5+5 1.0 93.5 1.0 86.00 1.50 85.00
CCP-8175 WG (Formulation-2) 48+105+6 0.0 100 0.0 100 0.0 100
CCP-8175 WG (Formulation-2) 56+122.5+
7 0.0 100 0.0 100 0.0 100

Treatments Dose (a.i/ha) Percent control of broad leave weeds (Mean 0.25

sqm) over UTC

Portulaca spp Parthenium spp Amaranthus spp.
Live popu latio n % control Live
populati
on % control Live
populatio
n % control
CCP-8175 WG (Formulation-3) 52.8 + 114 ) + 6 1.0 94.11 2.5 73.68 2.0 85.71
CCP-8175 WG (Formulation-3 (55 + 119) + 6.25 0.0 100.0 0.0 100 1.0 92.85
CCP-8175 WG (Formulation-3) 57.2 + 124) + 6.5 0.0 100.0 0.0 100 0.0 100
Formulation-1 CCP-8175 WG Quizalofop 8% + Oxyfluorfen 17.5%
Formulation-2 CCP-8175 WG Quizalofop 8% + Oxyfluorfen 17.5% + Vegetable oil 1%
Formulation-3 CCP-8175 WG Quizalofop 8.8% + Oxyfluorfen 19% + Vegetable
5 oil 1%
Table-3(c): Effect of herbicidal treatment on weed density of Broad leaves weeds at 21 DAA

Dose

Percent control of Broad leaves weeds (Mean 0.25 sqm)

(a.i/ha) over UTC
Treatments
Lactuca spp. Trianthema spp. Alternanthera spp.
Live pop. %control Live pop. %control Live pop. %contro 1
Untreated 14 - 12.5 - 11 -
Quizalofop ethyl 5 % EC 50 12 14.28 9.0 28.0 10 9.09
Oxyfluorfen 23.5% EC 199.8 2.5 82.14 3.0 76.0 2.0 81.81
CCP-8175 WG (Form-1) 40+87.5 3.0 78.57 3.5 72.0 4.5 59.09
CCP-8175 WG (Form-1) 48 + 105 2.5 82.14 3.0 76.0 3.5 68.18
CCP-8175 WG (Form-1) 56 + 122.5 1.0 92.85 2.0 84.0 2.5 77.27
CCP-8175 WG (Form-2) 40+87.5+5 3.5 75.0 4.0 68.0 2.5 77.27
CCP-8175 WG (Form-2) 48 + 105+6 2.0 85.71 2.0 84.0 1.0 90.90
CCP-8175 WG (Form-2) 56 + 122.5+7 1.5 89.28 1.0 92.0 1.0 90.90
CCP-8175 WG (Form-3) (52.8 + 114 ) + 6 2.0 85.71 1.0 92.0 1.0 90.90

CCP-8175 WG (Form-3) (55 + 119) + 6.25 0.0 100 0.0 100 0.0 100
CCP-8175 WG (Form-3) 57.2 + 124) + 6.5 0.0 100 0.0 100 0.0 100
Formulation-1 CCP-8175 WG Quizalofop 8% + Oxyfluorfen 17.5%
Formulation-2 CCP-8175 WG Quizalofop 8% + Oxyfluorfen 17.5% + Vegetable oil 1%
Formulation-3 CCP-8175 WG Quizalofop 8.8% + Oxyfluorfen 19% + Vegetable
5 oil 1%
EXAMPLE 3: Evaluation of phytotoxicity of the present composition in Onion crop
Visual observations were recorded at 3, 7 and 10 days after application (DAA) of
testing the presently disclosed composition and certain other reference
10 products. The parameters observed were leaf injury on tip/surface, necrosis, vein
clearing, epinasty, hyponasty and wilting. The score scale (1-10) followed for leaf injury on tips/surface is depicted in the table below:
Table 4: Phytotoxicity symptoms scoring and rating for leaf injury on tip/surface

Leaf injury on tips/surface Rating
0-10% 1
11-20% 2
21-30% 3

31-40% 4
41-50% 5
51-60% 6
61-70% 7
71-80% 8
81-90% 9
91-100% 10
The observations were recorded as below:
Table-4(a) Phytotoxic effect of various treatments on Onion crop 3 DAA.

Treatments Treatment details Dose a.i./hectare 3 DAA

L N V E H w
T-1 Untreated
T-2 Quizalofop ethyl 5 % EC 50 0 0 0 0 0 0
T-3 Oxyfluorfen 23.5% EC 199.8 0 0 0 0 0 0
T-4 CCP-8175 WG (Form-1) 40+87.5 0 0 0 0 0 0

Treatments Treatment details Dose a.i./hectare 3 DAA

L N V E H w
T-5 CCP-8175 WG (Form-1) 48 + 105 0 0 0 0 0 0
T-6 CCP-8175 WG (Form-1) 56 + 122.5 0 0 0 0 0 0
T-7 CCP-8175 WG (Form-2) 40+87.5+5 0 0 0 0 0 0
T-8 CCP-8175 WG (Form-2) 48 + 105+6 0 0 0 0 0 0
T-9 CCP-8175 WG (Form-2) 56 + 122.5+7 3 0 0 0 0 0
T-10 CCP-8175 WG (Form-3) (52.8 + 114 ) + 6 0 0 0 0 0 0
T-ll CCP-8175 WG (Form-al (55 + 119) + 6.25 0 0 0 0 0 0
T-12 CCP-8175 WG (Form-al 57.2 + 124) + 6.5 0 0 0 0 0 0
DAA - Days after application L- Leaf injury on tips/surface, N-Necrosis, V- Vein clearing, E- Epinasty, H- Hyponasty, W-wilting.
Table-(4b) Phytotoxic effect of various treatments on Onion crop after 7 DAA

Treatments

Treatment details

Dose

7 DAA

a.i./hectare L N V E H w
T-l Untreated
T-2 Quizalofop ethyl 5 % EC 50 0 0 0 0 0 0
T-3 Oxyfluorfen 23.5% EC 199.8 0 0 0 0 0 0
T-4 CCP-8175 WG (Form-1) 40+87.5 0 0 0 0 0 0
T-5 CCP-8175 WG (Form-1) 48 + 105 0 0 0 0 0 0
T-6 CCP-8175 WG (Form-1) 56 + 122.5 0 0 0 0 0 0
T-7 CCP-8175 WG (Form-2) 40+87.5+5 0 0 0 0 0 0
T-8 CCP-8175 WG (Form-2) 48 + 105+6 0 0 0 0 0 0
T-9 CCP-8175 WG (Form-2) 56 + 122.5+7 0 0 0 0 0 0
T-10 CCP-8175 WG (Form-3) (52.8 + 114 ) + 6 0 0 0 0 0 0
T-ll CCP-8175 WG (Form-3) (55 + 119) + 6.25 0 0 0 0 0 0
T-12 CCP-8175 WG (Form-3) 57.2 + 124) + 6.5 0 0 0 0 0 0
DAA - Days after application,
L- Leaf injury on tips/surface, N-Necrosis, V- Vein clearing, E- Epinasty, H-Hyponasty, W-wilting

Table-(4c) Phytotoxic effect of various treatments on Onion crop after 10 DAA

Treatments Treatment details Dose a.i./hectare 10 DAA

L N V E H w
T-l Untreated
T-2 Quizalofopethyl5% EC 50 0 0 0 0 0 0
T-3 Oxyfluorfen 23.5% EC 199.8 0 0 0 0 0 0
T-4 CCP-8175 WG (Form-1) 40+87.5 0 0 0 0 0 0
T-5 CCP-8175 WG (Form-1) 48 + 105 0 0 0 0 0 0
T-6 CCP-8175 WG (Form-1) 56 + 122.5 0 0 0 0 0 0
T-7 CCP-8175 WG (Form-2) 40+87.5+5 0 0 0 0 0 0
T-8 CCP-8175 WG (Form-2) 48 + 105+6 0 0 0 0 0 0
T-9 CCP-8175 WG (Form-2) 56 + 122.5+7 0 0 0 0 0 0
T-10 CCP-8175 WG (Form-3) (52.8 +114)+ 6 0 0 0 0 0 0
T-ll CCP-8175 WG (Form-3) (55+ 119)+ 6.25 0 0 0 0 0 0
T-12 CCP-8175 WG (Form-3) 57.2+ 124)+ 6.5 0 0 0 0 0 0
DAA - Days after application,
L- Leaf injury on tips/surface, N-Necrosis, V- Vein clearing, E- Epinasty, H-Hyponasty, W-wilting.

Result and observations:
The prominent grassy weed species in the weedy plot at the time of application
were on narrow leaves weed Echinocloa spp., Eleucine indica, Brachia spp. While
among the broad leaves weed the major weed flora included Portulaca spp.,
5 Parthenium spp., and Amaranthus spp.
It is clearly observed that application of the present novel herbicide Trimix coded
as CCP-8175 WG (Quizalofop ethyl 8.8% + oxyfluorfen 19% + Vegetable oil 1%
WG) at a dosage of 55 + 119 + 6.25 gm active ingredient (a.i) per hectare
provided effective control of both the narrow leaved weeds (94.73%) and broad
10 leaved weeds (100%) at 2-4 leaves stage of weeds without showing any
phytotoxicity related symptoms on onion crop.

I/We Claim:
1) A synergistic herbicidal composition comprising:
(a) Quizalofop including its agriculturally acceptable salts and esters thereof.
(b) Oxyfluorfen; and
(c) Vegetable oil

2) The herbicidal composition as claimed in claim 1, wherein the agriculturally acceptable form or ester of Quizalofop is Quizalofop ethyl.
3) The herbicidal composition as claimed in claim 1, wherein the amount of Quizalofop ranges from 1-15% w/w, the amount of Oxyfluorfen ranges from 5-35% w/w, and the amount of Vegetable oil ranges from 1-10% w/w.
4) The herbicidal composition of claim 3, wherein the amount of Quizalofop is 8.8% w/w, amount of Oxyfluorfen is 19% w/w, and amount of Vegetable oil is 1% w/w.
5) The herbicidal composition as claimed in claim 1, wherein said herbicidal composition is formulated in a form selected from the group consisting of emulsifiable concentrate (EC) wettable powder (WP), soluble (Liquid) concentrate (SL), Soluble powder (SP), Capsule suspension (CS) Water dispersible granules (WDG), Micro-emulsions (ME), Wettable granules (WG) and Suspension concentrate (SC) .
6) The herbicidal composition as claimed in claim 5, wherein the said herbicidal composition is formulated as or is in the form of Wettable granules (WG).
7) The herbicidal composition as claimed in claim 6, wherein said herbicidal composition further comprises agriculturally acceptable excipients selected from the group comprising at least an anti-freezing agent, at least a

surfactant, at least a dispersing agent, at least a wetting agent, at least a defoamer, at least a thickener, at least an antibacterial agent, demineralized water, and combinations thereof.
8) The herbicidal composition as claimed in claim 7, wherein said at least a dispersing agent is present in an amount 6-10% w/w, at least a wetting agent is present in an amount 4-8%, at least a antifoaming is present in an amount of 0.20-0.5%w/w, at least a carrier is present in an amount 5-10%w/w, at least a filler is present in an amount 2-6% w/w, and water to make up to 100% w/w.
9) A process of preparing a composition as claimed in claim 7 comprising the following steps:
i) Adding at least one wetting agent, dispersing agent, fillers and Quizalofop technical and mixing to obtain a homogeneous mix.
ii) Adding Oxyfluorfen technical and Vegetable oil to the mixture of step (0-
iii) Adding rest of the adjuvants to the mixture of step (ii) to obtain a homogeneous mass.
iv) Blending and grinding the homogeneous mass obtained in step (iii)
v) Grinding the homogenous mass obtained in step (iv) until a mean particle size in the range of 1-10 micron is obtained.
vi) Again blending the mass obtained in step (v) (Post mixing blending) to obtain the homogeneous mass.
vii) Preparing a dough by taking the homogenous mass obtained in step (vi)

viii) Preparing the granules by passing the mass through an extruder.
ix) Drying the granules obtained in step (viii) in hot air or oven to obtain the wettable granule (WG).