HETEROCYCLYLCOMPOUNDS
FIELD OF THE INVENTION
The present invention relates to anticancer compounds, their pharmaceutically
acceptable salts, combinations with suitable medicament and pharmaceutical
compositions thereof containing one or more such compounds, and methods of
treating various cancers.
CROSS-REFERENCE TO A RELATED APPLICATION
The present application claims the benefit of Indian Provisional Patent Application
No. 0288/KOL/2012, filed 14* March 2012, the disclosure of which is
incorporated herein by reference in its entirety.
BACKGROUND OF THE INVENTION
Cancer cells possess certain characteristics that allow them a growth advantage.
These include six main alterations in cell physiology such as self-sufficiency in
growth signals, insensitivlty to growth-inhibitory signals, evasion of apoptosis,
indefinite proliferative potential, sustained angiogenesis, tissue invasion and
metastasis (Hanahan and Weinberg, Cell, 2000, Vol. 100, 57-70). These changes
are triggered by genomic instability and inflammation which generates a
microenvlronment conducive for tumor growth. In addition to the above mentioned
traits, reprogramming of cellular energy metabolism and evasion of immune
destruction has also been observed in a majority of cancers.
The enhanced survival in cancer cells is further potentiated by the presence of
aberrantly activated signalling pathways. A large majority of cancers are known to
have mutations in growth factor signalling cascades that lead to constitutive
activation of these pathways. Such constitutive activations has been observed in
growth factor receptors which include but are not limited to epidermal growth
factor receptor - EGFR, fibroblast growth factor receptor - FGFR, Hepatocyte
growth factor receptor - HGF, etc. Furthermore, activating mutations have been
reported in certain receptor as well as non receptor tyrosine kinases which include
but are not limited to MET receptor tyrosine kinase, EGFR-tyrosine kinase, Bcr-Abl
tyrosine kinase, Src tyrosine kinase etc. Activation of Ser-Thr kinases such as Ras
and lipid kinases such as PI3-kinases also leads to oncogenesis. Chronic activation
of the growth factor/cytokine/hormone-associated signalling leads to activation of
immediate downstream components such as Src, Ras, PI3-kinase, etc. These
kinases further activate effectors such as MEK, ERK, AKT, eventually leading to
activation of transcription factors that endow the cells with a high proliferative
potential, improved survival, subversion of metabolic pathways and inhibition of
apoptosis (Hanahan and Weinberg, Cell, 2000, Vol. 100, 57-70; Hanahan and
Weinberg Cell 201 1, Vol. 144, 646-674)
MEK kinase (Mitogen Activated Protein Kinase Kinase (MAPKK)) is an important
component of the Ras-RAF-MEK-ERK cell survival pathway. The Ras pathway is
activated by binding of growth factors, cytokines, and hormones to their cognate
receptors. In cancer cells, this pathway is, however, constitutively activated and
leads to increased cancer cell survival, cell proliferation, angiogenesis and
metastasis. The tumors that show constitutive activation of the Ras or the MEK
kinase include but are not limited to those of the colon, pancreas, breast, brain,
ovary, lungs and skin (Sebolt-Leopold and Herrera, Nat. Rev. Cancer 2004, 4 937-
947; Fukazawa et al., Mol. Cancer Ther. 2002, Vol. 1, 303-309). Activation of Ras
(due to upstream signalling or as a result of activating point mutations in the Ras
oncogene) lead to the phosphorylation and activation of Raf kinase that in turn
phosphorylate and activate MEK kinase. MEK1/2 kinase phosphorylates and
activates the ERKl/2 kinase (also referred to as MAP Kinase) that further
phosphorylates and regulates the function of proteins such as Mcl- 1, Bim and Bad
that are involved in cell survival and apoptosis. Thus, activation of this
phosphorylation mediated cascade leads to enhanced cell proliferation, cell
survival, decreased cell death that are necessary for initiation and maintenance of
the tumorigenic phenotype (Curr. Opin. Invest. Drugs, 2008, 9, 614).
The Ras-Raf-MEK-ERK cascade plays a pivotal role in survival and proliferation of
cancer cells. As such, inhibition of this pathway at any of these levels would lead to
the inhibition of cancer cell growth, proliferation and survival. Indeed, it has
already been reported that inhibition of Ras or Raf leads to inhibition of tumor
growth in animal models as well as in cancer patients. However, the success with
these inhibitors has been limited to only certain types of cancers (e.g. Sorafenib
which inhibits Raf kinase has been approved for renal cell carcinoma). Hence,
inhibiting MEK is a novel approach towards controlling this pathway in cancer
cells. Moreover, the possibility of designing allosteric inhibitors also allows
enhanced selectivity that is crucial for decreasing the toxic effects associated with
kinase inhibitors.
The MEK-ERK Pathway is activated in numerous inflammatory conditions (Kyriakis
and Avruch, 1996, Vol. 271, No. 40, pp. 24313-24316; Hammaker et al., J .
Immunol. 2004, 172, 1612-1618), including rheumatoid arthritis, inflammatory
bowel disease and COPD. MEk regulates the biosynthesis of the inflammatory
cytokines TNF, IL-6 and IL- 1. It has been shown that MEK inhibitors interfere with
the production/secretion of these cytokines. Array BioPharma has developed a
first- in-class MEK inhibitor (ARRY 438162) and initiated clinical trials in
rheumatoid arthritis (RA) patients.
International patent applications WO/2003/053960, WO/2005/023251,
WO/2005/121 142, WO/2005/051906, WO/2010/121646 describe MEK
inhibitors.
BRIEF SUMMARY OF THE INVENTION
The present invention provides anticancer compounds of the general formula (I),
their pharmaceutically acceptable salts, combinations with suitable medicament
and pharmaceutical compositions thereof and use thereof in treating various
cancers.
Wherein, R -R5 are described in detail below.
The compounds of the present inventions are potent inhibitors of MEK and show
tumor regression effect with promisingly less side effects.
DETAILED DESCRIPTION OF THE INVENTION
The present invention relates to heteroaryl compounds of the general formula I,
their pharmaceutically acceptable salts, their combinations with suitable
medicament and pharmaceutical compositions thereof. The present invention also
includes processes of preparation of the compounds and their use in methods of
treatment. The compounds are of formula (I) below:
wherein,
R1 is selected from the group consisting of hydrogen, substituted- or
unsubstituted-alkyl, substituted- or unsubstituted- cycloalkyl, and substituted- or
unsubstituted- heterocyclyl;
R2 is selected from the group consisting of -R -E, -SO2R7, and -C(=0)R 8;
R3 is selected from the group consisting of hydrogen, substituted- or
unsubstituted- alkyl, and substituted- or unsubstituted- cycloalkyl;
R4 is selected from the group consisting of hydrogen, halogen, substituted- or
unsubstituted-alkyl, and substituted- or unsubstituted- cycloalkyl;
R5 is substituted- or unsubstituted- aryl, wherein the substituents are selected
from the group consisting of Ra and Rb;
Ra and Rb are each independently selected from the group consisting of hydrogen,
halogen and haloalkyl;
R6 is selected from the group consisting of direct bond, -[C(Rc)Rd]nNR9 , -
[C(Rc)Rd]nO-, -NHC(=0)[C(R c)Rd]p , -S(0) 2NH-, -NHC(=0)[CR (R )]NR9-, -
NHC(=0)[CR (R )]0-, and -NHS(0) 2- ;
R and Rd are each independently selected from the group consisting of hydrogen
and substituted- or unsubstituted alkyl;
E is four membered heterocyclic ring substituted- or unsubstituted- with alkyl,
halogen, -C(=0)OR and -OR ;
Re is selected from the group consisting of hydrogen, substituted- or
unsubstituted-alkyl and substituted or unsubstituted cycloalkyl;
R7 is selected from the group consisting of substituted- or unsubstitutedcycloalkyl,
and substituted- or unsubstituted- cycloalkenyl;
R8 is selected from the group consisting of substituted- or unsubstitutedalkyl,
substituted- or unsubstituted- alkenyl, substituted- or unsubstitutedalkynyl,
substituted- or unsubstituted- cycloalkyl, and substituted- or
unsubstituted- cycloalkenyl;
R9 is selected from the group consisting of hydrogen, substituted- or unsubstitutedalkyl,
substituted- or unsubstituted- alkenyl, substituted- or unsubstitutedalkynyl,
substituted- or unsubstituted- cycloalkyl and substituted or
unsubstituted- cycloalkenyl;
n is an integer selected from the group consisting of 0, 1 and 2 ;
p is an integer selected from 0 and 1;
when the alkyl group and alkenyl group is substituted, the alkyl group and alkenyl
group is substituted with 1 to 4 substituents independently selected from the
group consisting of oxo, halogen, nitro, cyano, perhaloalkyl, cycloalkyl, aryl,
heteroaryl, heterocyclyl, -OR 10b , -SO 2R 10a , -C(=O)OR 10a , -OC(=O)R 0 ,
C(=0)N(H)R o,-OR 0a , -C(=0)N(alkyl)R , -N(H)C(=O)R 0a , -N(H)R , -N(alkyl)R -
N(H)C (=0)N(H)R10 , -N(H)C(=0)N(alkyl)R10 , -NH-S02-alkyl, and -NH-S02-cycloalkyl;
when the cycloalkyl group and cycloalkenyl group is substituted, the cycloalkyl
group and cycloalkenyl group is substituted with 1 to 3 substituents independently
selected from the group consisting of oxo, halogen, nitro, cyano, alkyl, alkenyl,
perhaloalkyl, aryl, heteroaryl, heterocyclyl, -OR 10b , -SO 2R 10a , -C(=O)R 10a , -
C(=O)OR 0a , -OC(=O)R 0a , -C(=0)N(H)R , -C(=0)N(alkyl)R , -N(H)C(=O)R 0a , -
N(H)R , -N(alkyl)R , -N(H)C (=0)N(H)R , and -N(H)C(=0)N(alkyl)R , -NH-S02-alkyl,
and -NH-S02-cycloalkyl;
when the aryl group is substituted, the aryl group is substituted with 1 to 3
substituents independently selected from the group consisting of halogen, nitro,
cyano, hydroxy, alkyl, alkenyl, perhaloalkyl, cycloalkyl, cycloalkenyl, heterocycle, -
O-alkyl, -O-perhaloalkyl, -N(alkyl)alkyl, -N(H)alkyl, -NH2, -S0 2-alkyl, -S0 2-
perhaloalkyl, -N(alkyl)C(=0)alkyl, -N(H)C(=0)alkyl, -C(=0)N(alkyl)alkyl,
C(=0)N(H)alkyl, -C(=0)NH2, -S0 2N(alkyl)alkyl, -S0 2N(H)alkyl, -S0 2NH2, -NH-S0 2-
alkyl, and -NH-S0 2-cycloalkyl;
when the heteroaryl group is substituted, the heteroaryl group is substituted with
1 to 3 substituents independently selected from the group consisting of halogen,
nitro, cyano, hydroxy, alkyl, alkenyl, perhaloalkyl, cycloalkyl, cycloalkenyl,
heterocycle, -O-alkyl, O-perhaloalkyl, -N(alkyl)alkyl, -N(H)alkyl, -NH2, -S0 2-alkyl, -
S0 2-perhaloalkyl, -N(alkyl)C(=0)alkyl, -N(H)C(=0)alkyl, -C(=0)N(alkyl)alkyl, -
C(=0)N(H)alkyl, -C(=0)NH2, -S0 2N(alkyl)alkyl, -S0 2N(H)alkyl, -S0 2NH2, -NH-S0 2-
alkyl, and -NH-S0 2-cycloalkyl;
when the heterocyclyl group is substituted, the heterocyclyl group is substituted
with 1 to 3 substituents. When the substituents are on a ring carbon of the
'heterocycle', the substituents are independently selected from the group consisting
of halogen, nitro, cyano, oxo, alkyl, alkenyl, perhaloalkyl, cycloalkyl, cycloalkenyl,
aryl, heteroaryl, heterocyclyl, -OR10b , -C(=O)OR10a , -OC(=O)R10a, -C(=0)N(H)R °, -
-N(H)Rio, -N(alkyl)Rio, -
N(H)C(=0)N(alkyl)R10 . When the heterocyclic group is substituted on a ring
nitrogen of the 'heterocycle', the substituents are selected from the group
consisting of alkyl, alkenyl, cycloalkyl, cycloalkenyl, aryl, heteroaryl, -SO2R10a , -
C(=O)R 0a , C(=O)OR 0a -C(=0)N(H)R , -C(=0)N(alkyl)R , -NH-S0 2-alkyl, and -NHS0
2-cycloalkyl;
R10 is selected from the group consisting of hydrogen, alkyl, alkenyl, cycloalkyl,
cycloalkenyl, aryl, heteroaryl, and heterocyclyl;
Rioa S selected from the group consisting of alkyl, alkenyl, perhaloalkyl, cycloalkyl,
cycloalkenyl, aryl, heteroaryl, and heterocyclyl;
R10b is selected from the group consisting of hydrogen, alkyl, alkenyl, perhaloalkyl,
cycloalkyl, cycloalkenyl, aryl, heteroaryl, and heterocyclyl;
In certain embodiments, R1 is selected from the group consisting of hydrogen,
substituted- or unsubstituted- alkyl, and substituted- or unsubstituted- cycloalkyl.
In other embodiments, R1 is selected from the group consisting of hydrogen, methyl
and cyclopropyl.
In certain embodiments, R3 is substituted- or unsubstituted- alkyl.
In other embodiments, R3 is methyl.
In certain embodiments, R4 is substituted- or unsubstituted- alkyl.
In other embodiments, R4 is methyl.
In certain embodiments, R5 is substituted- or unsubstituted- phenyl, wherein the
substituents are independently selected from Ra and Rb.
In certain embodiments, Ra and Rb are particularly selected from hydrogen, halogen
and haloalkyl.
In other embodiments, Ra and Rb are independently fluorine or iodine.
In certain embodiments, R6 is selected from the group consisting of direct bond, -
[C(Rc)Rd]nNR9 , -[C(Rc)Rd]nO-, and -NHC(=0)[C(Rc)Rd]P .
In other embodiments, R6 is selected from the group consisting of direct bond, -
NH-, -0-, -CH2O-, and -NHC(=0)-.
In certain embodiments, E is selected from the group consisting of substituted- or
unsubstituted- 3-oxetane, 1-azetidine, l-azetidine-2-one and 3-azetidine; wherein
substituents are independently selected from methyl, fluoro, -C(=0)OR e, and -ORe.
In certain embodiments, Re is selected from hydrogen and substituted- or
unsubstitued-alkyl.
In other embodiments, Re is selected from hydrogen, tert-butyl, and
In certain embodiments, R7 is substituted- or unsubstituted- cycloalkyl.
In other embodiments, R7 is cyclopropyl.
In certain embodiments, R8 is substituted- or unsubtituted- cycloalkyl.
In other embodiments, R8 is cyclopropyl.
In certain embodiments, R9 is hydrogen.
In one embodiment, the present invention is a compound of formula la:
(la)
wherein,
R , R3, R4, Re, E , Ra and Rb are as defined in formula (I).
In another embodiment, the present invention is a compound of formula (lb):
Wherein;
R1, R3 , R4 , R7 , Ra and R are as defined in formula (I);
In another embodiment, the present invention is a compound of formula (Ic):
Wherein:
R , R3, R4, R8, Ra and Rb are as defined in formula (I).
General terms used in any of the formulae herein can be defined as follows;
however, the meaning stated should not be interpreted as limiting the scope of the
term per se.
The term "alkyl", as used herein, means a straight chain or branched hydrocarbon
containing from 1 to 20 carbon atoms. Preferably the alkyl chain may contain 1 to
10 carbon atoms. More preferably alkyl chain may contain up to 6 carbon atoms.
Representative examples of alkyl include, but are not limited to, methyl, ethyl, npropyl,
iso-propyl, n-butyl, sec-butyl, iso-butyl, tert-butyl, n-pentyl, isopentyl,
neopentyl, and n-hexyl.
The term "alkenyl" as used herein, means an 'alkyl' group as defined hereinabove
containing 2 to 20 carbon atoms and containing at least one double bond.
Representative examples of alkenyl include, but are not limited to, pent-2-enyl,
hex-3-enyl, allyl, vinyl, and the like.
'Alkyl', 'alkenyl' as defined hereinabove may be substituted with one or more
substituents selected independently from the group comprising of oxo, halogen,
nitro, cyano, haloalkyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, -OR10b , -SO2R10a , -
C(=O)R 0a , -C(=O)OR 0 , -OC(=O)R 0a , -C (=0)N(H)R ° , -C(=0)N(alkyl)R ° , -
N(H)C(=O)R 0a , -N(H)R , -N(alkyl)R °, -N(H)C (=0)N(H)R °, -N(H)C(=0)N(alkyl)R ° , -
NH-S02 -alkyl and -NH-SCh-cycloaikyl; wherein, R 10 is selected from the group
consisting of hydrogen, alkyl, alkenyl, cycloalkyl, cycloalkenyl, aryl, heteroaryl, and
heterocyclyl; R 10a is selected from the group consisting of alkyl, alkenyl, haloalkyl,
perhaloalkyl, cycloalkyl, cycloalkenyl, aryl, heteroaryl, heterocyclyl; R 10b is selected
from the group consisting of hydrogen, alkyl, alkenyl, haloalkyl, perhaloalkyl,
cycloalkyl, cycloalkenyl, aryl, heteroaryl, and heterocyclyl.
The term "haloalkyl" means alkyl, as the case may be, substituted with one or
more halogen atoms, where alkyl groups are as defined above. The term "halo" is
used herein interchangeably with the term "halogen" and means F, CI, Br or I.
Examples of "haloalkyl" include but are not limited to trifluoromethyl,
difluoromethyl, 2,2,2-trifluoroethyl, pentafluoroethyl, pentachloroethyl, 4,4, 4-
trifluorobutyl, 4,4-diiluorocyclohexyl, chloromethyl, dichloromethyl,
trichloromethyl, 1-bromoethyl and the like. The term "perhaloalkyl" group is
defined hereinabove wherein all the hydrogen atoms of the said alkyl group are
substituted with halogen, exemplified by triiluoromethyl, pentailuoroethyl and the
like.
The term "cycloalkyl" as used herein, means a monocyclic, bicyclic, or tricyclic
non-aromatic ring system containing from 3 to 14 carbon atoms, preferably
monocyclic cycloalkyl ring containing 3 to 6 carbon atoms. Examples of monocyclic
ring systems include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl,
and cyclooctyl. Bicyclic ring systems are also exemplified by a bridged monocyclic
ring system in which two non-adjacent carbon atoms of the monocyclic ring are
linked by an alkylene bridge. Representative examples of bicyclic ring systems
include, but are not limited to, bicyclo[3.1.1]heptane, bicyclo[2.2. l]heptane,
bicyclo[2.2.2]octane, bicyclo[3.2.2]nonane, bicyclo[3.3. l]nonane, and
bicyclo[4.2.1]nonane, bicyclo[3.3.2]decane, bicyclo[3.1.0] hexane,
bicyclo[410]heptane, bicyclo[3. 2.0]heptanes, octahydro-lH-indene. Tricyclic ring
systems are also exemplified by a bicyclic ring system in which two non-adjacent
carbon atoms of the bicyclic ring are linked by a bond or an alkylene bridge.
Representative examples of tricyclic-ring systems include, but are not limited to,
tricyclo[3.3.1.0 3 7]nonane and tricyclo[3.3.1.1 3 7]decane (adamantane) . The term
cycloalkyl also include spiro systems wherein one of the ring is annulated on a
single carbon atom such ring systems are exemplified by spiro [2. 5 ]octane,
spiro[4.5]decane, spiro [bicyclo [4. 1.0]heptane-2, 1'-cyclopentane] , hexahydro-2'Hspiro
[cyclopropane- 1,1'-pentalene] .
The term "cycloalkenyl" as used herein, means a cycloalkyl group as defined
above containing at least one double bond.
'cycloalkyl' and 'cycloalkenyl' as defined hereinabove may be substituted- or
unsubstituted- with one or more substituents selected independently from the
group consisting of oxo, halogen, nitro, cyano, hydroxyl, hydroxyalkyl, alkyl,
alkenyl, perhaloalkyl, aryl, heteroaryl, heterocyclyl, -OR 10b , -SO2R10a , -C(=O)R10a , -
N(H)Rio, -N(alkyl)Rio, -NH-S0 2-alkyl and
-NH-S02 -cycloalkyl; wherein, R 10 is selected from the group consisting of hydrogen,
alkyl, alkenyl, cycloalkyl, cycloalkenyl, aryl, heteroaryl, and heterocyclyl; R 10a is
selected from the group consisting of alkyl, alkenyl, perhaloalkyl, cycloalkyl,
cycloalkenyl, aryl, heteroaryl, heterocyclyl; R 10b is selected from the group
consisting of hydrogen, alkyl, alkenyl, perhaloalkyl, cycloalkyl, cycloalkenyl, aryl,
heteroaryl, and heterocyclyl.
The term "aryl" refers to a monovalent monocyclic, bicyclic or tricyclic aromatic
hydrocarbon ring system. Examples of aryl groups include phenyl, naphthyl,
anthracenyl, fluorenyl, indenyl, azulenyl, and the like. Aryl group also include
partially saturated bicyclic and tricyclic aromatic hydrocarbons such as
tetrahydro-naphthalene. The said aryl group also includes aryl rings fused with
heteroaryl or heterocyclic rings such as 2,3-dihydro-benzo[l,4]dioxin-6-yl; 2,3-
dihydro-benzo[l,4]dioxin-5-yl; 2,3-dihydro-benzofuran-5-yl; 2,3-dihydrobenzofuran-
4-yl; 2,3-dihydro-benzofuran-6-yl; 2,3-dihydro-benzofuran-6-yl; 2,3-
dihydro-lH-indol-5-yl; 2,3-dihydro-lH-indol-4-yl; 2,3-dihydro-lH-indol-6-yl; 2,3-
dihydro-lH-indol-7-yl; benzo[l,3]dioxol-4-yl; benzo[l,3]dioxol-5-yl; 1,2,3,4-
tetrahydroquinolinyl; 1,2,3,4-tetrahydroisoquinolinyl; 2,3-dihydrobenzothien-4-yl,
2-oxoindolin-5-yl.
Aryl as defined hereinabove may be substituted- or unsubstituted- with one or
more substituents selected independently from the group consisting of halogen,
nitro, cyano, hydroxy, alkyl, alkenyl, perhaloalkyl, cycloalkyl, cycloalkenyl,
heterocycle, -O-alkyl, -O-perhaloalkyl, -N(alkyl)alkyl, -N(H)alkyl, -NH2, -S0 2-alkyl, -
SCb-perhaloalkyl, -N(alkyl)C(=0)alkyl, -N(H)C(=0)alkyl, -C(=0)N(alkyl)alkyl, -
C(=0)N(H)alkyl, -C(=0)NH2, -S0 2N(alkyl)alkyl, -S0 2N(H)alkyl, -S0 2NH2, -NH-S0 2-
alkyl and -NH-S0 2-cycloalkyl;
The term "heteroaryl" refers to a 5-14 membered monocyclic, bicyclic, or tricyclic
ring system having 1-4 ring heteroatoms selected from O, N, or S, and the
remainder ring atoms being carbon (with appropriate hydrogen atoms unless
otherwise indicated), wherein at least one ring in the ring system is aromatic.
Heteroaryl groups may be substituted-or unsubstituted- with one or more
substituents. In one embodiment, 0, 1, 2, 3, or 4 atoms of each ring of a heteroaryl
group may be substituted by a substituent. Examples of heteroaryl groups include
pyridyl, 1-oxo-pyridyl, furanyl, thienyl, pyrrolyl, oxazolyl, oxadiazolyl, imidazolyl,
thiazolyl, isoxazolyl, quinolinyl, pyrazolyl, isothiazolyl, pyridazinyl, pyrimidinyl,
pyrazinyl, triazinyl. triazolyl, thiadiazolyl, isoquinolinyl, benzoxazolyl, benzofuranyl,
indolizinyl, imidazopyridyl, tetrazolyl, benzimidazolyl, benzothiazolyl,
benzothiadiazolyl, benzoxadiazolyl, indolyl, azaindolyl, imidazopyridyl, quinazolinyl,
purinyl, pyrrolo [2,3 ]pyrimidinyl, pyrazolo[3,4]pyrimidinyl, and benzo (b)thienyl, 2,3-
thiadiazolyl, lH-pyrazolo[5, 1-c]- 1,2,4-triazolyl, pyrrolo[3,4-d]- 1,2,3-triazolyl,
cyclopentatriazolyl, 3H-pyrrolo[3,4-c] isoxazolyl and the like.
Heteroaryl as defined hereinabove may be substituted- or unsubstituted- with one
or more substituents selected independently from the group consisting of halogen,
nitro, cyano, hydroxy, alkyl, alkenyl, perhaloalkyl, cycloalkyl, cycloalkenyl,
heterocycle, -O-alkyl, O-perhaloalkyl, -N(alkyl)alkyl, -N(H)alkyl, -NH2, -S0 2-alkyl, -
S0 2-perhaloalkyl, -N(alkyl)C(=0)alkyl, -N(H)C(=0)alkyl, -C(=0)N(alkyl)alkyl, -
C(=0)N(H)alkyl, -C(=0)NH2, -S0 2N(alkyl)alkyl, -S0 2N(H)alkyl, -S0 2NH2, -NH-S0 2-
alkyl and -NH-S0 2-cycloalkyl.
The term "heterocycle" or "heterocyclic" as used herein, means a 'cycloalkyl' group
wherein one or more of the carbon atoms replaced by -0-, -S-, -S(0 2)-, -S(O)-, -
N(Rm)-, -Si(Rm)R - , wherein, Rm and R are independently selected from the group
consisting of hydrogen, alkyl, alkenyl, alkynyl, aryl, heteroaryl, cycloalkyl, and
heterocyclyl. The heterocycle may be connected to the parent molecular moiety
through any carbon atom or any nitrogen atom contained within the heterocycle.
Representative examples of monocyclic heterocycle include, but are not limited to,
azetidinyl, azepanyl, aziridinyl, diazepanyl, 1,3-dioxanyl, 1,3-dioxolanyl, 1,3-
dithiolanyl, 1,3-dithianyl, imidazolinyl, imidazolidinyl, isothiazolinyl,
isothiazolidinyl, isoxazolinyl, isoxazolidinyl, morpholinyl, oxadiazolinyl.
oxadiazolidinyl, oxazolinyl, oxazolidinyl, piperazinyl, piperidinyl, pyranyl,
pyrazolinyl, pyrazolidinyl. pyrrolinyl, pyrrolidinyl, tetrahydrofuranyl,
tetrahydrothienyl, thiadiazolinyl, thiadiazolidinyl, thiazolinyl, thiazolidinyl,
thiomorpholinyl, 1.1-dioxidothiomorpholinyl (thiomorpholine sulfone). thiopyranyl,
and trithianyl. Representative examples of bicyclic heterocycle include, but are not
limited to 1,3-benzodioxolyl, 1,3-benzodithiolyl, 2,3-dihydro-l,4-benzodioxinyl, 2,3-
dihydro-l-benzofuranyl, 2,3-dihydro-l-benzothienyl, 2,3-dihydro-l H-indolyl and
1,2,3,4-tetrahydroquinolinyl. The term heterocycle also include bridged
heterocyclic systems such as azabicyclo [3. 2.1]octane, azabicyclo[3.3.1]nonane and
the like.
Heterocyclyl group may be substituted- or unsubstituted- on ring carbons with one
or more substituents selected independently from the group consisting of halogen,
nitro, cyano, oxo, alkyl, alkenyl, perhaloalkyl, cycloalkyl, cycloalkenyl, aryl,
heteroaryl, heterocyclyl, -OR10b , -C(=O)OR10a , -OC(=O)R10a , -C(=0)N(H)R10 , -
C(=0)N(alkyl)R10 , -N(H)C(=O)R10a, -N(H)R10 , -N(alkyl)R10 , -N(H)C(=0)N(H)R10 , -
N(H)C(=0)N(alkyl)R10 ; the substituents on ring nitrogen of 'heterocycle' is selected
from alkyl, alkenyl, cycloalkyl, cycloalkenyl, aryl, heteroaryl, -SO2R10a , -C(=O)R10a ,
C(=O)OR 0a -C(=0)N(H)R , -C(=0)N(alkyl)R , -NH-S0 2-alkyl and -NH-S0 2-
cycloalkyl; R10a is selected from alkyl, alkenyl, perhaloalkyl, cycloalkyl,
cycloalkenyl, aryl, heteroaryl, heterocyclyl; R10b is selected from hydrogen, alkyl,
alkenyl, perhaloalkyl, cycloalkyl, cycloalkenyl, aryl, heteroaryl, and heterocyclyl.
The term xo' means a divalent oxygen (=0) attached to the parent group. For
example oxo attached to carbon forms a carbonyl, oxo substituted on cyclohexane
forms a cyclohexanone, and the like.
The term 'annulated' means the ring system under consideration is either
annulated with another ring at a carbon atom of the cyclic system or across a bond
of the cyclic system as in the case of fused or spiro ring systems.
The term 'bridged' means the ring system under consideration contain an alkylene
bridge having 1 to 4 methylene units joining two non adjuscent ring atoms.
It should be understood that the formulas (I), (la), (lb) and (Ic) structurally
encompasses all tautomers and pharmaceutically acceptable salts that may be
contemplated from the chemical structure of the genera described herein.
A compound its racemates, tautomers and pharmaceutically acceptable salt thereof
as described hereinabove wherein the compound of general formula I, (la), (lb) and
(Ic) is selected from the group consisting of:
l-(3-(cyclopropylsulfonyl)phenyl)-5-((2-fluoro-4-iodophenyl)amino)-6,8-dimethyl
pyrido [4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 1)
3-cyclopropyl-l-(2-fluoro-4-iodophenyl)-5-((3-(3-hydroxyazetidin-lyl)
phenyl)amino) -6,8-dimethylpyrido[2,3-d]pyrimidine-2,4,7(lH,3H,8H)-trione
(Compound 2)
3-cyclopropyl-l-(3-(cyclopropylsulfonyl)phenyl)-5-((2-fluoro-4-
iodophenyl)amino)-6,8- dimethylpyrido [4 ,3-d ]pyrimidine- 2,4,7(1H,3H,6H)-trione
(Compound 3)
3-cyclopropyl-5-((2-fluoro-4-iodophenyl)amino)-6,8-dimethyl-l-(3-(2-
oxoazetidin-l-yl)phenyl)pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione
(Compound 4)
3-cyclopropyl-5-((2-fluoro-4-iodophenyl)amino)-6,8-dimethyl-l-(3-(oxetan-3-
ylamino) phenyl)pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 5)
tert-butyl 3-((3-(3-cyclopropyl-5-((2-fluoro-4-iodophenyl)amino)-6,8-dimethyl-
2,4,7-trioxo-3,4,6,7-tetrahydropyrido[4,3-d]pyrimidin- 1(2H)-
yl)phenyl)carbamoyl) azetidine- 1-carboxylate (Compound 6)
3-cyclopropyl-5-((2-fluoro-4-iodophenyl)amino)-6,8-dimethyl-l-(3-(oxetan-3-
yloxy) phenyl)pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 7)
l-(3-(azetidin-l-yl)phenyl)-3-cyclopropyl-5-((2-fluoro-4-iodophenyl)amino)-6,8-
dimethyl pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 8)
3-cyclopropyl-5-((2-fluoro-4-iodophenyl)amino)-l-(3-(3-hydroxyoxetan-3-
yl)phenyl)-6,8-dimethylpyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione
(Compound 9)
N-(3-(3-cyclopropyl-5-((2-iluoro-4-iodophenyl)amino)-6,8-dimethyl-2,4,7-trioxo-
3,4,6,7-tetrahydropyrido[4,3-d]pyrimidin-l(2H)-yl)phenyl)-3-methyloxetane-3-
carboxamide (Compound 10)
l-(3-(cyclopropanecarbonyl)phenyl)-3-cyclopropyl-5-((2-iluoro-4-
iodophenyl)amino)-6,8-dimethylpyrido [4 ,3-d ]pyrimidine-2,4,7(1H,3H,6H)-trione
(Compound 11)
l-(3-(cyclopropylsulfonyl)phenyl)-5-((2-fluoro-4-iodophenyl)amino)-3,6,8-
trimethyl pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 12)
5-((2-fluoro-4-iodophenyl)amino)-3,6,8-trimethyl-l-(3-((oxetan-3-yloxy)methyl)
phenyl)pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 13)
5-((2-iluoro-4-iodophenyl)amino)-l-(3-(3-hydroxyoxetan-3-yl)phenyl)-3,6,8-
trimethylpyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 14)
5-((2-iluoro-4-iodophenyl)amino)-3,6,8-trimethyl-l-(3-(oxetan-3-yloxy)phenyl)
pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 15)
l-(3-(azetidin-l-yl)phenyl)-5-((2-iluoro-4-iodophenyl)amino)-3,6,8-
trimethylpyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 16)
5-((2-iluoro-4-iodophenyl)amino)-3,6,8-trimethyl-l-(3-(oxetan-3-
ylamino)phenyl) pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound
17)
N-(3-(5-((2-iluoro-4-iodophenyl)amino)-3,6,8-trimethyl-2,4,7-trioxo-3,4,6,7-
tetrahydropyrido[4,3-d]pyrimidin-l(2H)-yl)phenyl)-3-methyloxetane-3-
carboxamide (Compound 18)
2-((l-(3-(5-((2-fluoro-44odophenyl)amino)-3,6,8-trimethyl-2,4,7-trioxo-3,4,6,7-
tetra hydropyrido[4,3-d]pyrimidin- 1(2H)-yl)phenyl)azetidin-3-yl)oxy)acetamide
(Compound 19)
5-((2-iluoro-4-iodophenyl)amino)-l-(3-(3-iluorooxetan-3-yl)phenyl)-3,6,8-
trimethyl pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 20)
N-(3-(3-cyclopropyl-5-((2-iluoro-4-iodophenyl)amino)-6,8-dimethyl-2,4,7-trioxo-
3,4,6, 7-tetrahydropyrido [4 ,3-d ]pyrimidin- 1(2H)-yl)phenyl)azetidine- 3-
carboxamide (Compound 21)
The present disclosure provides a method for inhibiting MEK enzymes comprising
contacting said MEK enzyme with a composition comprising a compound of I, la,
lb, Ic, their tautomeric forms or their pharmaceutically acceptable salts, sufficient
to inhibit said enzyme, wherein said enzyme inhibited MEK kinase, which occurs
within cell.
The invention also provides a method of treatment of a MEK mediated disorder in
an individual suffering from said disorder, comprising administering to said
individual an effective amount of a composition comprising a compound of formula
I, la, lb, Ic, their tautomeric forms or their pharmaceutically acceptable salts. The
said method of treatment may also be combined with an additional therapy such as
radiation therapy, chemotherapy, or combination thereof.
MEK mediated disorders as stated above include inflammatory diseases, infections,
autoimmune disorders, stroke, ischemia, cardiac disorder, neurological disorders,
fibrogenetic disorders, proliferative disorders, hyperproliferative disorders, tumors,
leukemias, neoplasms, cancers, carcinomas, metabolic diseases and malignant
diseases.
The invention further provides a method for the treatment or prophylaxis of a
proliferative disease in an individual comprising administering to said individual an
effective amount of a composition comprising a compound of formula I, la, lb, Ic,
their tautomeric forms or their pharmaceutically acceptable salts. The proliferative
disease includes cancer, psoriasis, restenosis, autoimmune disease, or
atherosclerosis.
The invention also provides a method for the treatment or prophylaxis of an
inflammatory disease in an individual comprising administering to said individual
an effective amount of a composition comprising a compound of formula I, la, lb,
Ic, their tautomeric forms or their pharmaceutically acceptable salts. The
inflammatory disease includes rheumatoid arthritis or multiple sclerosis.
The invention also provide a method for degrading, inhibiting the growth of or
killing cancer cells comprising contacting the cells with an amount of a
composition effective to degrade, inhibit the growth of or kill cancer cells, the
composition comprising a compound of formula I, la, lb, Ic, their tautomeric forms
or their pharmaceutically acceptable salts.
The invention also provide a method of inhibiting tumor size increase, reducing the
size of a tumor, reducing tumor proliferation or preventing tumor proliferation in
an individual in need thereof comprising administering to said individual an
effective amount of a composition to inhibit tumor size increase, reduce the size of
a tumor, reduce tumor proliferation or prevent tumor proliferation, the composition
comprising a compound of formula I, la, lb, Ic, their tautomeric forms or their
pharmaceutically acceptable salts.
The MEK-ERK pathway is activated in numerous inflammatory conditions (Kyriakis
and Avruch 1996, Vol. 271, No. 40, pp. 24313-24316; Hammaker et al., J
Immunol 2004; 172; 16 12- 16 18), including rheumatoid arthritis, inflammatory
bowel disease and COPD. MEK regulates the biosynthesis of the inflammatory
cytokines TNF, IL-6 and IL- 1. It has been shown that MEK inhibitors interfere with
the production/secretion of these cytokines.
The present invention describes the inhibitors of MEK kinase for treatment of
disorders that are driven by hyperactivation, abnormal activation, constitutive
activation, gain-of-function mutation of the MEK kinase and/or its substrate
kinases that include but are not limited to ERK. Such disorders encompass
hyperproliferative disorders that include but are not limited to psoriasis, keloids,
hyperplasia of the skin, benign prostatic hyperplasia (BPH), solid tumors such as
cancers of the respiratory tract (including but not limited to small cell and nonsmall
cell lung carcinomas), brain (including but not limited to glioma,
meduUoblastoma, ependymoma, neuroectodermal and pineal tumors), breast
(including but not limited to invasive ductal carcinoma, invasive lobular carcinoma,
ductal- and lobular carcinoma in situ), reproductive organs (including but not
limited to prostate cancer, testicular cancer, ovarian cancer, endometrial cancer,
cervical cancer, vaginal cancer, vulvar cancer, and sarcoma of the uterus) , digestive
tract (including but not limited to esophageal, colon, colorectal, gastric, gall
blabber, pancreatic, rectal, anal, small intestine and salivary gland cancers),
urinary tract (including but not limited to bladder, ureter, kidney, renal, urethral
and papillary renal cancers), eye (including but not limited to intraocular
melanoma, and retinoblastoma), liver (including but not limited to hepatocellular
carcinoma, and cholangiocarcinoma) , skin (including but not limited to melanoma,
squamous cell carcinoma, Kaposi's sarcoma, Merkel cell skin cancer, nonmelanoma
skin cancer), head and neck (including but not limited to laryngeal,
nasopharyngeal, hypopharyngeal, oropharyngeal cancer, lip and oral cavity cancer
and squamous cell cancer), thyroid, parathyroid, and their metastases. The
hyperrproliferative disorders also include, leukemias (including but not limited to
acute lymphoblastic leukemia, acute myeloid leukemia, chronic melogenous
leukemia, chronic lymphocytic leukemia, and hairy cell leukemia), sarcomas
(including but not limited to soft tissue sarcoma, osteosarcoma, lymphosarcoma,
rhabdomyosarcoma), and lymphomas (including but not limited to non-Hodgkin's
lymphoma, AIDS-related lymphoma, cutaneous T cell lymphoma, Burkitt's
lymphoma, Hodgkin's disease, and lymphoma of the central nervous system).
The present invention describes the inhibitors of MEK kinase for treatment of
certain disorders involving aberrant regulation of the mitogen extracellular kinase
activity including but not limited to hepatomegaly, heart failure, cardiomegaly,
diabetes, stroke, Alzheimer's disease, cystic fibrosis, septic shock or asthma.
The present invention describes the inhibitors of MEK kinase for treatment of
diseases and disorders associated with aberrant, abnormal and/or excessive
angiogenesis. Such disorders associated with angiogenesis include but are not
limited to, tumor growth and metastases, ischemic retinal vein occlusion, diabetic
retinopathy, macular degeneration, neovascular glaucoma, psoriasis,
inflammation, rheumatoid arthritis, vascular graft restenosis, restenosis and instent
restenosis.
The compounds mentioned in this invention can be used as a single (sole)
therapeutic agent or in combination with other active agents, including
chemotherapeutic agents and anti-inflammatory agents. Such combinations
include but are not limited to combining the MEK kinase inhibitors with an ti
mitotic agents, anti- antiangiogenic agents, alkylating agents, antihyperproliferative
agents, antimetabolites, DNA-intercalating agents, cell cycle
inhibitors, kinase inhibitors, growth factor inhibitors, enzyme inhibitors,
topoisomerase inhibitors, biological response modifiers or anti- hormones.
The term 'room temperature' denotes any temperature ranging between about 20°C
to about 40°C, except and otherwise it is specifically mentioned in the specification.
The intermediates and the compounds of the present invention may be obtained in
pure form in a manner known per se, for example, by distilling off the solvent in
vacuum and re-crystallizing the residue obtained from a suitable solvent, such as
pentane, diethyl ether, isopropyl ether, chloroform, dichloromethane, ethyl acetate,
acetone or their combinations or subjecting it to one of the purification methods,
such as column chromatography (e.g., flash chromatography) on a suitable support
material such as alumina or silica gel using eluent such as dichlorome thane, ethyl
acetate, hexane, methanol, acetone and their combinations. Preparative LC-MS
method is also used for the purification of molecules described herein.
Salts of compound of formula I can be obtained by dissolving the compound in a
suitable solvent, for example in a chlorinated hydrocarbon, such as methyl chloride
or chloroform or a low molecular weight aliphatic alcohol, for example, ethanol or
isopropanol, which was then treated with the desired acid or base as described in
Berge S.M. et al. "Pharmaceutical Salts, a review article in Journal of
Pharmaceutical sciences volume 66, page 1-19 (1977)" and in handbook of
pharmaceutical salts properties, selection, and use by P.H.Einrich Stahland
Camille G.wermuth, Wiley- VCH (2002). Lists of suitable salts can also be found in
Remington's Pharmaceutical Sciences, 18th ed., Mack Publishing Company, Easton,
PA, 1990, p. 1445, and Journal of Pharmaceutical Science, 66, 2-19 (1977). For
example, the salt can be of an alkali metal (e.g., sodium or potassium), alkaline
earth metal (e.g., calcium), or ammonium.
The compound of the invention or a composition thereof can potentially be
administered as a pharmaceutically acceptable acid-addition, base neutralized or
addition salt, formed by reaction with inorganic acids, such as hydrochloric acid,
hydrobromic acid, perchloric acid, nitric acid, thiocyanic acid, sulfuric acid, and
phosphoric acid, and organic acids such as formic acid, acetic acid, propionic acid,
glycolic acid, lactic acid, pyruvic acid, oxalic acid, malonic acid, succinic acid,
maleic acid, and fumaric acid, or by reaction with an inorganic base, such as
sodium hydroxide, potassium hydroxide. The conversion to a salt is accomplished
by treatment of the base compound with at least a stoichiometric amount of an
appropriate acid. Typically, the free base is dissolved in an inert organic solvent
such as diethyl ether, ethyl acetate, chloroform, ethanol, methanol, and the like,
and the acid is added in a similar solvent. The mixture is maintained at a suitable
temperature (e.g., between 0 °C and 50 °C). The resulting salt precipitates
spontaneously or can be brought out of solution with a less polar solvent.
The compounds of formula I of the present invention can exist in tautomeric forms,
such as keto-enol tautomers. Such tautomeric forms are contemplated as an
objective of this invention and such tautomers may be in equilibrium or
predominant in one of the forms.
The prodrugs can be prepared in situ during the isolation and purification of the
compounds, or by separately reacting the purified compound with a suitable
derivatizing agent. For example, hydroxy groups can be converted into esters via
treatment with a carboxylic acid in the presence of a catalyst. Examples of
cleavable alcohol prodrug moieties include substituted or unsubstituted, branched
or unbranched lower alkyl ester moieties, e.g., ethyl esters, lower alkenyl esters, dilower
alkylamino lower-alkyl esters, e.g., dimethylaminoethyl ester, acylamino
lower alkyl esters, acyloxy lower alkyl esters (e.g., pivaloyloxymethyl ester), aryl
esters, e.g., phenyl ester, aryl-lower alkyl esters, e.g., benzyl ester, substituted- or
unsubstituted, e.g., with methyl, halo, or methoxy substituents aryl and aryl-lower
alkyl esters, amides, lower-alkyl amides, di-lower alkyl amides, and hydroxy
amides.
The term "prodrug" denotes a derivative of a compound, which derivative, when
administered to warm-blooded animals, e.g. humans, is converted into the
compound (drug). The enzymatic and/or chemical hydrolytic cleavage of the
compounds of the present invention occurs in such a manner that the proven drug
form (parent carboxylic acid drug) is released, and the moiety or moieties split off
remain nontoxic or are metabolized so that nontoxic metabolic products are
produced. For example, a carboxylic acid group can be esterified, e.g., with a
methyl group or ethyl group to yield an ester. When an ester is administered to a
subject, the ester is cleaved, enzymatically or non-enzymatically, reductively,
oxidatively, or hydrolytically, to reveal the anionic group. An anionic group can be
esterified with moieties (e.g., acyloxymethyl esters) which are cleaved to reveal an
intermediate compound which subsequently decomposes to yield the active
compound.
The inhibitors mentioned in the present invention can be combined with
antiinflammatory agents or agents that show therapeutic benefit for conditions
including but not limited to hepatomegaly, heart failure, cardiomegaly, diabetes,
stroke, alzheimer's disease, cystic fibrosis, septic shock or asthma, diabetic
retinopathy, ischemic retinal vein occlusion, macular degeneration, neovascular
glaucoma, psoriasis, inflammation, rheumatoid arthritis, restenosis, in-stent
restenosis, and vascular graft restenosis.
The term "aberrant kinase activity" refers to any abnormal expression or activity of
the gene encoding the kinase or of the polypeptide t encodes. Examples of such
aberrant kinase activity Include but are not limited to over-expression of the gene
or polypeptide, gene amplification, mutations that produce constltutlvely active or
hyperactive kinase activity, gene mutations, deletions, substitutions, additions,
and the like.
Thus the present Invention further provides a pharmaceutical composition,
containing the compounds of the general formula (I) as defined above, Its
tautomeric forms, Its pharmaceutically acceptable salts n combination with the
usual pharmaceutically acceptable carriers, diluents, exclplents and the like.
The pharmaceutically acceptable carrier (or exclplent) s preferably one that s
chemically Inert to the compound of the Invention and one that has no detrimental
side effects or toxicity under the conditions of use. Such pharmaceutically
acceptable carriers or exclplents Include saline (e.g., 0.9% saline), Cremophor EL
(which s a derivative of castor oil and ethylene oxide available from Sigma
Chemical Co., St. Louis, MO) (e.g., 5% Cremophor EL/5% ethanol/90% saline, 10%
Cremophor EL/90% saline, or 50% Cremophor EL/50% ethanol), propylene glycol
(e.g., 40% propylene glycol/ 10% ethanol/50% water), polyethylene glycol (e.g., 40%
PEG 400/60% saline), and alcohol (e.g., 40% ethanol/60% water). A preferred
pharmaceutical carrier s polyethylene glycol, such as PEG 400, and particularly a
composition comprising 40% PEG 400 and 60% water or saline. The choice of
carrier will be determined n part by the particular compound chosen, as well as by
the particular method used to administer the composition. Accordingly, there s a
wide variety of suitable formulations of the pharmaceutical composition of the
present Invention.
The following formulations for oral, aerosol, parenteral, subcutaneous,
Intravenous, Intraarterial, Intramuscular, Interperitoneal, rectal, and vaginal
administration are merely exemplary and are n no way limiting.
The pharmaceutical compositions can be administered parenterally, e.g.,
intravenously, intraarterially, subcutaneously, intradermally, intrathecally, or
intramuscularly. Thus, the invention provides compositions for parenteral
administration that comprise a solution of the compound of the invention dissolved
or suspended in an acceptable carrier suitable for parenteral administration,
including aqueous and non-aqueous, isotonic sterile injection solutions.
Overall, the requirements for effective pharmaceutical carriers for parenteral
compositions are well known to those of ordinary skill in the art. See
Pharmaceutics and Pharmacy Practice, J.B. Lippincott Company, Philadelphia, PA,
Banker and Chalmers, eds., pages 238-250 (1982), and ASHP Handbook on
Injectable Drugs, Toissel, 4th ed., pages 622-630 (1986). Such compositions
include solutions containing anti-oxidants, buffers, bacteriostats, and solutes that
render the formulation isotonic with the blood of the intended recipient, and
aqueous and non-aqueous sterile suspensions that can include suspending agents,
solubilizers, thickening agents, stabilizers, and preservatives. The compound can
be administered in a physiologically acceptable diluent in a pharmaceutical carrier,
such as a sterile liquid or mixture of liquids, including water, saline, aqueous
dextrose and related sugar solutions, an alcohol, such as ethanol, isopropanol (for
example in topical applications), or hexadecyl alcohol, glycols, such as propylene
glycol or polyethylene glycol, dimethylsulf oxide, glycerol ketals, such as 2,2-
dimethyl-l,3-dioxolane-4-methanol, ethers, such as poly(ethyleneglycol) 400, an
oil, a fatty acid, a fatty acid ester or glyceride, or an acetylated fatty acid glyceride,
with or without the addition of a pharmaceutically acceptable surfactant, such as a
soap or a detergent, suspending agent, such as pectin, carbomers, methylcellulose,
hydroxypropylmethylcellulose, or carboxymethylcellulose, or emulsifying agents
and other pharmaceutical adjuvants.
Oils useful in parenteral formulations include petroleum, animal, vegetable, and
synthetic oils. Specific examples of oils useful in such formulations include
peanut, soybean, sesame, cottonseed, corn, olive, petrolatum, and mineral oil.
Suitable fatty acids for use in parenteral formulations include oleic acid, stearic
acid, and isostearic acid. Ethyl oleate and isopropyl myristate are examples of
suitable fatty acid esters.
Suitable soaps for use in parenteral formulations include fatty alkali metal,
ammonium, and triethanolamine salts, and suitable detergents include (a) cationic
detergents such as, for example, dimethyl dialkyl ammonium halides, and alkyl
pyridinium halides, (b) anionic detergents such as, for example, alkyl, aryl, and
olefin sulfonates, alkyl, olefin, ether, and monoglyceride sulfates, and
sulfosuccinates, (c) nonionic detergents such as, for example, fatty amine oxides,
fatty acid alkanolamides, and polyoxyethylene polypropylene copolymers, (d)
amphoteric detergents such as, for example, alkyl^-aminopropionates, and 2-
alkyl-imidazoline quaternary ammonium salts, and (e) mixtures thereof.
The parenteral formulations typically will contain from about 0.5% or less to about
25% or more by weight of a compound of the invention in solution. Preservatives
and buffers can be used. In order to minimize or eliminate irritation at the site of
injection, such compositions can contain one or more nonionic surfactants having
a hydrophile-lipophile balance (HLB) of from about 12 to about 17. The quantity of
surfactant in such formulations will typically range from about 5% to about 15%
by weight. Suitable surfactants include polyethylene sorbitan fatty acid esters,
such as sorbitan monooleate and the high molecular weight adducts of ethylene
oxide with a hydrophobic base, formed by the condensation of propylene oxide with
propylene glycol. The parenteral formulations can be presented in unit-dose or
multi-dose sealed containers, such as ampoules and vials, and can be stored in a
freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid
excipient, for example, water, for injections, immediately prior to use.
Extemporaneous injection solutions and suspensions can be prepared from sterile
powders, granules, and tablets.
Topical formulations, including those that are useful for transdermal drug release,
are well known to those of skill in the art and are suitable in the context of the
present invention for application to skin.
Formulations suitable for oral administration can consist of (a) liquid solutions,
such as an effective amount of a compound of the invention dissolved in diluents,
such as water, saline, or orange juice; (b) capsules, sachets, tablets, lozenges, and
troches, each containing a pre-determined amount of the compound of the
invention, as solids or granules; (c) powders; (d) suspensions in an appropriate
liquid; and (e) suitable emulsions. Liquid formulations can include diluents, such
as water and alcohols, for example, ethanol, benzyl alcohol, and the polyethylene
alcohols, either with or without the addition of a pharmaceutically acceptable
surfactant, suspending agent, or emulsifying agent. Capsule forms can be of the
ordinary hard- or soft-shelled gelatin type containing, for example, surfactants,
lubricants, and inert fillers, such as lactose, sucrose, calcium phosphate, and
cornstarch. Tablet forms can include one or more of lactose, sucrose, mannitol,
corn starch, potato starch, alginic acid, microcrystalline cellulose, acacia, gelatin,
guar gum, colloidal silicon dioxide, croscarmellose sodium, talc, magnesium
stearate, calcium stearate, zinc stearate, stearic acid, and other excipients,
colorants, diluents, buffering agents, disintegrating agents, moistening agents,
preservatives, flavoring agents, and pharmacologically compatible excipients.
Lozenge forms can comprise the compound ingredient in a flavor, usually sucrose
and acacia or tragacanth, as well as pastilles comprising a compound of the
invention in an inert base, such as gelatin and glycerin, or sucrose and acacia,
emulsions, gels, and the like containing, in addition to the compound of the
invention, such excipients as are known in the art.
A compound of the present invention, alone or in combination with other suitable
components, can be made into aerosol formulations to be administered via
inhalation. A compound or epimer of the invention is preferably supplied in finely
divided form along with a surfactant and propellant. Typical percentages of the
compounds of the invention can be about 0.01% to about 20% by weight,
preferably about 1% to about 10% by weight. The surfactant must, of course, be
nontoxic, and preferably soluble in the propellant. Representative of such
surfactants are the esters or partial esters of fatty acids containing from 6 to 22
carbon atoms, such as caproic, octanoic, lauric, palmitic, stearic, linoleic, linolenic,
olesteric and oleic acids with an aliphatic polyhydric alcohol or its cyclic anhydride.
Mixed esters, such as mixed or natural glycerides can be employed. The surfactant
can constitute from about 0. 1% to about 20% by weight of the composition,
preferably from about 0.25% to about 5%. The balance of the composition is
ordinarily propellant. A carrier can also be included as desired, e.g., lecithin, for
intranasal delivery. These aerosol formulations can be placed into acceptable
pressurized propellants, such as dichlorodiiluoromethane, propane, nitrogen, and
the like. They also can be formulated as pharmaceuticals for non-pressured
preparations, such as in a nebulizer or an atomizer. Such spray formulations can
be used to spray mucosa.
Additionally, the compound of the invention can be made into suppositories by
mixing with a variety of bases, such as emulsifying bases or water-soluble bases.
Formulations suitable for vaginal administration can be presented as pessaries,
tampons, creams, gels, pastes, foams, or spray formulas containing, in addition to
the compound ingredient, such carriers as are known in the art to be appropriate.
The concentration of the compound in the pharmaceutical formulations can vary,
e.g., from less than about 1% to about 10%, to as much as 20% to 50% or more by
weight, and can be selected primarily by fluid volumes, and viscosities, in
accordance with the particular mode of administration selected.
For example, a typical pharmaceutical composition for intravenous infusion could
be made up to contain 250 ml of sterile Ringer's solution, and 100 mg of at least
one compound of the invention. Actual methods for preparing parenterally
administrable compounds of the invention will be known or apparent to those
skilled in the art and are described in more detail in, for example, Remington's
Pharmaceutical Science (17th ed., Mack Publishing Company, Easton, PA, 1985).
It will be appreciated by one of ordinary skill in the art that, in addition to the
aforedescribed pharmaceutical compositions, the compound of the invention can
be formulated as inclusion complexes, such as cyclodextrin inclusion complexes,
or liposomes. Liposomes can serve to target a compound of the invention to a
particular tissue, such as lymphoid tissue or cancerous hepatic cells. Liposomes
can also be used to increase the half-life of a compound of the invention. Many
methods are available for preparing liposomes, as described in, for example, Szoka
et al., Ann. Rev. Biophys. Bioeng., 9, 467 (1980) and U.S. Patents 4,235,871,
4,501,728, 4,837,028, and 5,019,369.
The compounds of the invention can be administered in a dose sufficient to treat
the disease, condition or disorder. Such doses are known in the art (see, for
example, the Physicians' Desk Reference (2004)). The compounds can be
administered using techniques such as those described in, for example,
Wasserman et al., Cancer, 36, pp. 1258-1268 (1975) and Physicians' Desk
Reference, 58th ed., Thomson PDR (2004).
Suitable doses and dosage regimens can be determined by conventional rangefinding
techniques known to those of ordinary skill in the art. Generally, treatment
is initiated with smaller dosages that are less than the optimum dose of the
compound of the present invention. Thereafter, the dosage is increased by small
increments until the optimum effect under the circumstances is reached. The
present method can involve the administration of about 0.1 m g to about 50 mg of at
least one compound of the invention per kg body weight of the individual. For a 70
kg patient, dosages of from about 10 m g to about 200 mg of the compound of the
invention would be more commonly used, depending on a patient's physiological
response.
By way of example and not intending to limit the invention, the dose of the
pharmaceutically active agent(s) described herein for methods of treating or
preventing a disease or condition as described above can be about 0.001 to about 1
mg/kg body weight of the subject per day, for example, about 0.001 mg, 0.002 mg,
0.005 mg, 0.010 mg, 0.015 mg, 0.020 mg, 0.025 mg, 0.050 mg, 0.075 mg, 0.1 mg,
0.15 mg, 0.2 mg, 0.25 mg, 0.5 mg, 0.75 mg, or 1 mg/kg body weight per day. The
dose of the pharmaceutically active agent(s) described herein for the described
methods can be about 1 to about 1000 mg/kg body weight of the subject being
treated per day, for example, about 1 mg, 2 mg, 5 mg, 10 mg, 15 mg, 0.020 mg, 25
mg, 50 mg, 75 mg, 100 mg, 150 mg, 200 mg, 250 mg, 500 mg, 750 mg, or 1000
mg/kg body weight per day.
The terms "treat," "prevent," "ameliorate," and "inhibit," as well as words stemming
therefrom, as used herein, do not necessarily imply 100% or complete treatment,
prevention, amelioration, or inhibition. Rather, there are varying degrees of
treatment, prevention, amelioration, and inhibition of which one of ordinary skill in
the art recognizes as having a potential benefit or therapeutic effect. In this
respect, the disclosed methods can provide any amount of any level of treatment,
prevention, amelioration, or inhibition of the disorder in a mammal. For example,
a disorder, including symptoms or conditions thereof, may be reduced by, for
example, 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, or 10%.
Furthermore, the treatment, prevention, amelioration, or inhibition provided by the
inventive method can include treatment, prevention, amelioration, or inhibition of
one or more conditions or symptoms of the disorder, e.g., cancer. Also, for
purposes herein, "treatment," "prevention," "amelioration," or "inhibition" can
encompass delaying the onset of the disorder, or a symptom or condition thereof.
In accordance with the invention, the term subject includes an "animal" which in
turn includes a mammal such as, without limitation, the order Rodentia, such as
mice, and the order Lagomorpha, such as rabbits. In one aspectthat the mammals
are from the order Carnivora, including Felines (cats) and Canines (dogs). In
another aspect the mammals are from the order Artiodactyla, including Bovlnes
(cows) and Swine (pigs) or of the order Perssodactyla, including Equines (horses).
In a further aspect, the mammals are of the order Primates, Ceboids, or Simoids
(monkeys) or of the order Anthropoids (humans and apes). In yet another aspect,
the mammal is the human.
General Method of preparation
The compounds of general formula (I) where all the symbols are as defined earlier
can be prepared by methods given in below schemes or examples illustrated herein
below.
However, the disclosure should not be construed to limit the scope of the invention
arriving at compound of formula (I) disclosed hereinabove.
Scheme 1 (R is H)
Compound of formula (I) where R1 is H, can b e prepared as depicted in Scheme 1,
details of which are given below.
Scheme 1
Step-1
Compound of formula (II) where R1 is N-protecting group, can be converted to
compound of formula (III) by reacting compound of II (Z is any suitable leaving
group like CI, Br, I, -0(SO) 2(4-MePh), -0(SO) 2CH3, -0(SO) 2CF3 etc.) with R2NH2 in
presence of a suitable base like 2,6-Lutidine, l,8-Diazabicyclo[5.4.0]undec-7-ene
(DBU), K2C0 3, CsaCOs, NaH, KH, n-BuLi, lithium bis (trimethylsilyl) amide (LiHMDS)
etc., in a solvent like THF, DMF, DMSO etc., at temperature ranging from about -
78SC to about 150 C.
Step-2
Compound of formula- (III) where R1 is N-protecting group, can b e converted to
compound of formula- (IV) by reacting compound of formula (III) with suitable base
such as NaOMe, K2C0 3 etc. in a solvent like Methanol, Ethanol, THF, DMF etc. at
temperature ranging from about -78 SC to about 150 SC.
Step-3
Compound of formula- (IV) where R1 is N-protecting group, can be converted to
compound of formula- (I) by reacting compound of formula (IV) with suitable Ndeprotection
agents such as AICI3, Pd-C/H2 etc. in a solvent like Anisole, Toluene,
Xylene, THF, DMF, DMSO etc. at temperature ranging from about -78 SC to about
150 SC.
Scheme-2:
Compound of formula (I) where R1 is selected from the group consisting of
substituted- or unsubstituted- alkyl, substituted- or unsubstituted- alkenyl,
substituted- or unsubstituted- alkynyl, substituted- or unsubstituted- cycloalkyl,
substituted- or unsubstituted- cycloalkenyl, substituted- or unsubstituted- aryl,
substituted- or unsubstituted- heteroaryl, and substituted- or unsubstitutedheterocyclyl,
can be prepared as depicted in Scheme 2, details of which are given
below
Scheme 2
Step-1
Compound of formula (II) where where R1 is selected from substituted- or
unsubstituted- alkyl, substituted- or unsubstituted- alkenyl, substituted- or
unsubstituted- alkynyl, substituted- or unsubstituted- cycloalkyl, substituted- or
unsubstituted- cycloalkenyl, substituted- or unsubstituted- aryl, substituted- or
unsubstituted- heteroaryl, and substituted- or unsubstituted- heterocyclyl, can be
converted to compound of formula (III) by reacting compound of II (Z is any suitable
leaving group like CI, Br, I, -0(SO) 2(4-MePh), -0(SO) 2CH3, -0(SO) 2CF3 etc.) with
R NH2 in presence of a suitable base like 2,6-Lutldlne, 1,8-
Dlazablcyclo[5.4.0]undec-7-ene (DBU), K2C0 3, Cs2C0 3, NaH, KH, n-BuLl, lithium
bls(trimethylsllyl)amlde (L1HMDS) etc., n a solvent like THF, DMF, DMSO etc., at
temperature ranging from about -78 SC to about 150 SC.
Step-2
Compound of formula- (III) where R1 s selected from substituted- or unsubstltutedalkyl,
substituted- or unsubstltuted- alkenyl, substituted- or unsubstltutedalkynyl,
substituted- or unsubstltuted- cycloalkyl, substituted- or unsubstltutedcycloalkenyl,
substituted- or unsubstltuted- aryl, substituted- or unsubstltutedheteroaryl,
and substituted- or unsubstltuted- heterocyclyl,, can be converted to
compound of formula- (I) by reacting compound of formula (III) with suitable base
such as NaOMe, K2C0 3 etc. n a solvent like Methanol, Ethanol, THF, DMF etc. at
temperature ranging from about -78 SC to about 150 SC.
The Intermediates and the compounds of the present Invention are obtained n
pure form n a manner known per se, for example by distilling off the solvent n
vacuum and re- crystallizing the residue obtained from a suitable solvent, such as
pentane, diethyl ether, Isopropyl ether, chloroform, dlchloro methane, ethyl acetate,
acetone or their combinations or subjecting t to one of the purification methods,
such as column chromatography (e.g. flash chromatography) on a suitable support
material such as alumina or silica gel using eluent such as dlchlorome thane, ethyl
acetate, hexane, methanol, acetone and their combinations. Preparative LC-MS
method s also used for the purification of molecules described herein.
Salts of compound of formula I are obtained by dissolving the compound n a
suitable solvent, for example n a chlorinated hydrocarbon, such as methyl chloride
or chloroform or a low molecular weight aliphatic alcohol, for example, ethanol or
Isopropanol, which was then treated with the desired acid or base as described n
Berge S. M. et al. "Pharmaceutical Salts, a review article n Journal of
Pharmaceutical sciences volume 66, page 1-19 (1977)" and n handbook of
pharmaceutical salts properties, selection, and use by P.H.Einrich Stahland
Camille G.wermuth , Wiley- VCH (2002).
Examples
The following examples are provided to further illustrate the present invention and
therefore should not be construed in any way to limit the scope of the present
invention. All H MR spectra were determined in the solvents indicated and
chemical shifts are reported in d units downfield from the internal standard
tetramethylsilane (TMS) and interproton coupling constants are reported in Hertz
(Hz).
Unless otherwise stated, work-up includes distribution of the reaction mixture
between the organic and aqueous phase indicated within parentheses, separation
of layers and drying the organic layer over sodium sulphate, filtration and
evaporation of the solvent. Purification, unless otherwise mentioned, includes
purification by silica gel chromatographic techniques, generally using a mobile
phase with suitable polarity. The following abbreviations are used in the text:
DMSO-d6: Hexadeuterodimethyl sulfoxide; DMSO: Dimethylsulfoxide, DMF: N,Ndimethyl
formamide, DMA: Dimethylacetamide, THF: Tetrahydrofuran, TEA:
Trifluoroacetic acid, DAST: Diethylaminosulfur trifluoride; DCM: Dichlorome thane,
m-CPBA: meta-Chloroperoxybenzoic acid, EDC: l-Ethyl-3-(3-
dimethylaminopropyl)carbodiimide, J Coupling constant in units of Hz, RT or rt:
room temperature (22-26°C), Aq.: aqueous, AcOEt: ethyl acetate, equiv. or eq.:
equivalents and hr. or h : hour(s)
Intermediates:
Intermediate-i: Synthesis of 3-(cyclopropylsulfonyl)aniline
Step 1: Synthesis of 3-chloropropyl)(3-nitrophenyl)sulfane
To a suspension of 3-nitrobenzenethiol (2.3 g, 14.82 mmol) and NaOH (1. 186 g,
29.6 mmol) in Ethanol (40.0 ml) was added l-bromo-3-chloropropane (1.75 ml,
17.79 mmol). The resulting mixture was stirred at RT under inert atmosphere for
18 h . Solvent was evaporated under vacuum and the residue was partitioned
between DCM (200 ml) and water (100 ml). The organic layer was separated and
washed with brine (100 ml), dried over anhydrous sodium sulphate and evaporated
under vacuum. The residual oil was purified by column chromatography over
silicagel, using 15 % EtOAc in Hexane as eluent to afford (3-chloropropyl)(3-
nitrophenyl)sulfane (2.92 g, 12.60 mmol, 85 % yield).
Ή NMR(400 MHz,CDCl3) 8 8.14-8. 16 (m, 1H), 8.05-8.00 (m, 1H), 7.65-7.60 (m,
1H), 7.49-7.43 (m, 1H), 3.70 (t, J = 6.2 Hz, 2H), 3.2 (t, J = 7.2 Hz, 2H), 2.18-2.12
(m, 2H). GCMS: 231.04 [M+]
Step 2 : Synthesis of l-((3-chloropropyl)sulfonyl)-3-nitrobenzene
m-CPBA (7.45 g, 32.4 mmol) was added to a solution of (3-chloropropyl)(3-
nitrophenyl)sulfane (3 g, 12.95 mmol) in CHCI3 (50 mL). Resulting mixture was
stirred at RT for 18 hrs and filtered to remove most of the benzoic acid. The filtrate
was diluted with CHC13 (100 ml) and washed with 10 % aq. NaOH (100 ml).
Organic phase was dried over anhydrous sodium sulphate and evaporated under
vacuum. Residue was purified by silicagel column chromatography eluting with
30% EtOAc:Hexane to afford l-((3-chloropropyl)sulfonyl)-3-nitrobenzene (2.75 g).
H NMR(400 MHz,CDCl3) 8 8.79 (brs, 1H), 8.55 (d, J =8.0 Hz, 1H), 8.28 (d, J =7.6
Hz, 1H), 7.88-7.84(m, 1H), 3.68 (t, J = 6.0 Hz, 2H), 3.37 (t, J = 7.6 Hz, 2H), 2.32-
2.25 (m, 2H). GCMS: 262.96 [M+]
Step 3 : Synthesis of l-(cyclopropylsulfonyl)-3-nitrobenzene
Potassium tert-butoxide (2.13 g, 18.96 mmol) was added to a solution of l-((3-
chloropropyl)sulfonyl)-3-nitrobenzene (2 g, 7.58 mmol) in t-BuOH (10 ml) at RT.
Resulting solution was stirred at RT for 5 h . Solvent was evaporated under
vacuum. Residue was partitioned between EtOAc (150 ml) and water (150 ml).
Organic phase was removed and dried over sodium sulphate. Solvent was
evaporated under vacuum to obtain l -(cyclopropylsulfonyl)-3-nitrobenzene (1.206
g, 5.3 1 mmol, 70 % yield), which was carried forward to the next step without
further purification.
H NMR(400 MHz,CDCl3) 8 8.78 (brs, IH), 8.52 (d, J = 8.0 Hz, IH) , 8.26 (d, J =7.6
Hz, IH), 7.84-7.80 (m, IH) , 2.55-2.52 (m, IH) , 1.45- 1.38 (m, 2H), 1.15- 1.13 (m,
2H). GCMS: 227.0 1 [M+]
Step 4 : Synthesis of 3-(cyclopropylsulfonyl)aniline
Triethylsilane (14 ml, 88 mmol) was added dropwise to a suspension of 1-
(cyclopropylsulfonyl)-3-nitrobenzene (2 g, 8.80 mmol) and Pd/C (10%, 250 mg) in
MeOH (25 ml). Resulting suspension was stirred at RT for 20 min. and filtered
through celite. The filtrate was evaporated under vacuum and triturated in hexane
to obtain the crystals which were collected by filtration to afford 3-
(cyclopropylsulfonyl)aniline (1.44 gm).
Ή NMR(400 MHz,DMSO-d6) 8 7.24 (t, J = 8.0 Hz, IH), 7.03-7.02 (m, IH), 6.95-6.92
(m, IH), 6.84-6.8 1 (m, IH) , 5.66 (s, 2H) , 2.74-2.70 (m, IH) , 1.05-0.95 (m, 4H) .
GCMS: 197.03 [M+] .
Intermediate-ii: Synthesis of N-(3-aminophenyl)-3-methyloxetane-3-
carboxamide
Step-1: Synthesis of 3-methyl-N-(3-nitrophenyl) oxetane-3-carboxamide
To a stirred solution of 3-nitroaniline (0.297 g, 2.153 mmol) in pyridine (1 ml), was
added 3-methyloxetane-3-carboxylic acid (0.250 g, 2.153 mmol) and EDC.HC1
(0.6 19 g, 3.23 mmol). The reaction mixture was stirred at room temperature for 2
hrs. and then concentrated under vacuum, the residue was diluted with water (10
ml) and extracted with ethyl acetate (3 x 7ml). The combined organic layer was
washed with brine and water, dried over sodium sulfate and concentrated under
vacuum to afford the title compound (400 mg).
HNMR (400 MHz, CDC13): d 8.45 (t, 1H, J=2Hz), 8.06 (bs, 1H), 8.02-7.99 (m, 2H),
7.54 (t, 1H, J=4 Hz), 4.95 (d, 2H, J=6.4Hz), 4.65 (d, 2H, J=6.4Hz), 1.68 (s, 3H).
GCMS: 236 (M+)
Step-2: Synthesis of N-(3-aminophenyl)-3-methyloxetane-3-carboxamide
To a stirred solution of 3-methyl-N-(3-nitrophenyl) oxetane-3-carboxamide (0.5 g,
2.1 17 mmol) in Ethyl acetate (5 ml), 10% Pd/C (0.225 g) was added and the
reaction mixture was stirred room temperature for 10 hrs under hydrogen
atmosphere. The reaction mixture was filtered through celite and concentrated
under vacuum to afford the title product (0.400 g).
GCMS: 206 (M+).
Intermediate-iii: Synthesis of 3-(3-aminophenyl) oxetan-3-ol
Step-1: Synthesis of 3-(3-bromophenyl) oxetan-3-ol
To a stirred solution of l-bromo-3-iodobenzene (0.500 g, 1.767 mmol) in THF (5
ml), at 78 °C was added n-butyllithium (1.1 ml, 1.767 mmol). The reaction mixture
was stirred at same temperature for 1 hr, then oxetan-3-one (0.127 g, 1.767 mmol)
was added. The reaction mixture was stirred at -40 °C for one hour and saturated
ammonium chloride solution was added. The reaction mixture was extracted with
ethyl acetate (3 x 5 ml). Combined organic layer was dried over sodium sulfate and
concentrated under vacuum to afford the crude product which was purified by
column chromatography over silica gel using 30% ethyl acetate in hexane as eluent
to give the title product (80 mg).
HNMR (400 MHz, CDC13): d 7.80 (t, 1H, J= 2 Hz), 7.51-7.48 (m, 1H), 7.32 (t, 1H,
J=7.6Hz), 5.60-5.57 (m, 1H), 4.93-4.87 (m, 4H), 2.52 (s, 1H).
Step-2: Synthesis of 3-(3-aminophenyl) oxetan-3-ol
To a stirred mixture of 3-(3-bromophenyl)oxetan-3-ol (0.2 g, 0.873 mmol) in aq.
ammonia (1 ml), copper(II) oxide (0.069 g, 0.873 mmol) was added and the mixture
was heated at 90 °C for 24 hrs in a sealed tube. The reaction mixture was cooled to
room temperature and ethyl acetate (10 ml) was added, the reaction mixture was
filtered through celite and the filtrate was concentrated under vacuum to afford
title product (0.1 g).
iHNMR (400 MHz, CDC13) : d 7.21 (t, 1H, J=7.6Hz), 7.0-6.97 (m, 1H), 6.93-6.92 (m,
1H), 6.67-6.65 (m, 1H), 4.90 (s, 4H), 3.79 (bs, 2H), 3.02 (bs, 1H). GCMS: 165 (M+).
Intermediate-iv: Synthesis of 3-(azetidin-l-yl) aniline
Step-1: Synthesis of l-(3-nitrophenyl) azetidine
A stirred mixture of l-fluoro-3-nitrobenzene (0.305 g, 2.161 mmol), Azetidine .H
(0.2 g, 2. 161 mmol) and K2CO3 (0.747 g, 5.40 mmol) in DMSO (5 ml) was heated at
86 °C for 24 hrs. The reaction mixture was cooled to room temperature and water
was added (25 ml), the mixture was extracted with ethyl acetate (3X10 ml).
Combined organic layer was dried over sodium sulfate and concentrated under
vacuum to afford the crude compound, which was then purified by column
chromatography (40 mg).
HNMR (400 MHz, CDC13) : 7.55-7.52 (m, 1H), 7.31 (t, 1H, J= 8 Hz), 7.20 (t, 1H, J=
2.4 Hz), 6.70-6.67 (m, 1H), 3.97 (t, 4H, J= 7.6 Hz), 2.48-2.41 (m, 2H). GCMS: 178
(M+).
Step-2: Synthesis of 3-(azetidin-l-yl) aniline
To a stirred solution of l-(3-nitrophenyl)azetidine (0.040 g, 0.224 mmol) in Ethyl
acetate (2 ml) was added Pd/C (10%, O.Olmg) and the reaction mixture was heated
at 55 °C for 12 hrs under hydrogen atmosphere. The reaction mixture was cooled
to room temperature and diluted with ethyl acetate (10 ml) and the mixture was
filtered through celite, and the filtrate was concentrated under vacuum to afford
the title compound (30 mg, 90 %).
iHNMR (400 MHz, CDC13) : 7.02 (t, 1H, J= 8 Hz), 6.12-6.10 (m, 1H), 5.93-5.90 (m,
1H), 5.80-5.79 (m, 1H), 3.85 (t, 4H, J= 7.2 Hz), 3.59 (bs, 2H), 2.37-2.30 (m, 2H).
GCMS: 148 (M+)
Intermediate-v: Synthesis of tert-butyl 3-((3-
aminophenyl)carbamoyl)azetidine-1-carboxylate
Step-1: Synthesis of tert-butyl 3-((3-nitrophenyl)carbamoyl)azetidine-lcarboxylate
To 3-nitroaniline (0.137 g, 0.994 mmol) in pyridine (6 ml) were added l-(tertbutoxycarbonyl)
azetidine-3-carboxylic acid (0.5 g, 2.485 mmol) and EDC. HC1
(0.715 g, 3.73 mmol). After being stirred at room temperature for 2 hrs reaction
mixture was concentrated under vacuum. Diluted above reaction mixture with
water (10ml) extracted with 3X7ml chloroform: IPA (3:1). Washed organic layer with
brine and water, dried over sodium sulfate and concentrated to afford titled
compound (300 mg, 38 %).
ESI-MS m/z: 322 (M+l).
Step-2: Synthesis of tert-butyl 3-((3-aminophenyl)carbamoyl)azetidine-lcarboxylate
To a stirred solution of tert-butyl 3-((3-nitrophenyl)carbamoyl)azetidine-lcarboxylate
(0.3 g, 0.934 mmol) in methanol (10 ml) was added ammonium formate
(0.3 g, 4.76 mmol) and Pd/C (10%, 0.05 g) and the mixture was stirred at room
temperature for 10 hrs., since reaction was not complete so, Pd/C (0.05 g) and
ammonium formate (0.3 g, 4.76 mmol) were added again and the stirring was
continued for further 6 hrs. The reaction ixture was filtered through celite and the
filtrate was concentrated. The residue was purified by column chromatography
over silica gel using ethyl acetate in hexane as eluent to afford the title compound
(200 mg).
ESI-MS m/z: 292 (M+l)
Intermediate-vi: Synthesis of l-(3-aminophenyl) azetidin-3-ol
Step-1: Synthesis of l-(3-nitrophenyl)azetidin-3-ol
Azetidin-3-ol hydrochloride (1.0 g, 9.13 mmol) was taken in DMSO and l-fluoro-3-
nitrobenzene (1.0 g, 7.09 mmol) was added followed by K2C0 3 (2.449 g, 17.72
mmol). The reaction mixture was heated to 100 °C for 16 hrs. After completion of
the reaction, the reaction mixture was cooled and poured into water which was
then extracted with ethyl acetate (3 x 8ml). The organic layers were combined,
washed with water and brine, dried over sodium sulfate, and concentrated under
vacuum. The resulting crude product was purified by column chromatography to
yield the titled compound l-(3-nitrophenyl)azetidin-3-ol (0.73 g).
H NMR(400 MHz,CDCl3) 8 7.58 (dd, 1H, J= 1.6 and 8 Hz), 7.33 (t, 1H, 8 Hz) , 7.24
(t, 1H, J= 2 Hz), 6.73-6.7 1 (m, lH), 4.86-4.84 (m, 1H) , 4.28-4.26 (m, 2H), 3.80-3. 77
(m, 2H) , 2.18 (d, 1H, J=6.4 Hz) . ESI-MS [m/z = 194 [M+ l ]] .
Step-2: Synthesis of l-(3-aminophenyl) azetidin-3-ol
In ethyl acetate (10 ml) , l-(3-nitrophenyl)azetidin-3-ol (0.43 g, 2.2 14 mmol) was
taken and Pd/C (10%, 0.04 g) was added and the reaction mixture was stirred
under hydrogen atmosphere at room temperature for 10 hrs. After completion of
the reaction, the reaction mixture was filtered through celite, residue was washed
with ethyl acetate (3X5 ml) . Combined filtrate was concentrated under vacuum to
yield the title compound (0.35 g).
H NMR(400 MHz, CDC13) d 7.0 1 (t, 1H, J= 8 Hz), 6.15-6. 12 (m, 1H), 5.95-5.92 (m,
1H), 5.8 1 (t, IH, J= 2.4 Hz) , 4.73-4.69 (m, IH) , 4.16-4. 12 (m, 2H) , 3.66-3.62 (m,
4H), 1.73 (brs, IH) . ESI-MS [m/z = 164 [M+ l ]] .
Intermediate-vii: Synthesis of l-(3-aminophenyl)azetidin-2-one
To a mixture of l -(3-nitrophenyl)azetidin-2-one (600 mg, 3.12 mmol) , ammonium
formate (59 1 mg, 9.37 mmol) and methanol (40 ml) at 0°C, was added Pd/C (10%,
0.17 g) and the reaction mixture was stirred at 50°C for 3 hrs. The reaction
mixture was cooled to room temperature and filtered through celite, the filtrate was
concentrated under vacuum. The residue was taken in ethyl acetate and filtered
through celite and concentrated under vacuum to afford the yellow solid product
(230 mg) .
HNMR (400 MHz, CDC13): d 7.11 (t, IH, J=8 Hz) , 6.92-6.9 1 (m, IH) , 6.59-6.57 (m,
IH), 6.44-6.4 1 (m, IH) , 3.75 (bs, 2H), 3.60 (t, 2H, J = 4.4 Hz), 3.09 (t, 2H, J=4.4
Hz) .
Intermediate-viii: Synthesis of 3-(oxetan-3-yloxy)aniline
Step-1: Synthesis of 3-(3-nitrophenoxy)oxetane
Oxetan-3-ol (578 mg, 7.80 mmol) was taken in THF (8 ml) under nitrogen
atmosphere, cooled to 0°C, KOtBu (962 mg, 8.58 mmol) was added and the mixture
was stirred at same temperature for 30 min., l-fluoro-3-nitrobenzene (0.41 ml,
3.90 mmol) was added and the reaction mixture was stirred at room temperature
for 18 hrs. The reaction mixture was concentrated under vacuum and water (50
ml) was added, the mixture was extracted with ethyl acetate (3X 20 ml). Combined
organic layer was dried over sodium sulfate and the mixture was concentrated
under vacuum to afford the crude product, which was purified by column
chromatography to afford yellow solid product (350 mg) .
GCMS: 195 (M+)
Step-2: Synthesis of 3-(oxetan-3-yloxy)aniline
To a mixture of 3-(3-Nitrophenoxy)oxetane (0.350 g, 1.793 mmol), ammonium
formate (339 mg, 5.38 mmol) and methanol (40 ml) at 0°C, was added Pd/C (10%,
0.17 g) and the reaction mixture was stirred at 50°C for 3 hrs. The reaction
mixture was cooled to room temperature and filtered through celite, the filtrate was
concentrated under vacuum. The residue was taken in ethyl acetate and filtered
through celite and concentrated under vacuum to afford the yellow solid product
(260 mg).
Intermediate-ix: Synthesis of 2-((l-(3-aminophenyl)azetidin-3-
yl)oxy)acetamide
Step-1: Synthesis of l-(3-nitrophenyl)azetidin-3-ol
Azetidin-3-ol hydrochloride (1.863 g, 17.01 mmol), l-fluoro-3-nitrobenzene (2. g,
14.17 mmol) were taken in DMSO (20 ml) and K2C0 3 (4.90 g, 35.4 mmol) was
added to the mixture and the reaction mixture was heated at 110°C for 24 hrs. The
reaction mixture was cooled to room temperature, water was added and the
mixture was extracted with ethyl acetate (3X 75ml). Combined organic layer was
dried over sodium sulphate and concentrated under vacuum to afford the crude
product which was purified by column chromatography eluting with 0- 20% ethyl
acetate in hexane to afford the title product (1.1 g).
Step-2: Synthesis of 2-((l-(3-nitrophenyl)azetidin-3-yl)oxy)acetamide
1-(3-Nitrophenyl)azetidin-3-ol (1.2 g, 6. 18 mmol), 2-bromoacetamide (1.023 g, 7.42
mmol) were taken in DMF (20 ml) and 60% NaH (0.741 g, 18.5 mmol) was added
the to the mixture and the mixture was heated at 40 °C for 16 hrs. The reaction
mixture was cooled to room temperature, water was added and the mixture was
extracted with ethyl acetate (3X 75ml). Combined organic layer was dried over
sodium sulphate and concentrated under vacuum to afford the crude product
which was purified by column chromatography eluting with 0- 20% ethyl acetate in
hexane to afford the title product (0.8 g).
Step-3: Synthesis of 2-((l-(3-aminophenyl)azetidin-3-yl)oxy)acetamide
2-((l-(3-Nitrophenyl)azetidin-3-yl)oxy)acetamide (0.8 g, 3.18mmol) was taken in
methanol (20 ml) and at 0°C, Pd-C (10%, 0.07 g) was added. The reaction mixture
was stirred under hydrogen atmosphere at room temperature for 5 hrs. The
reaction mixture was filtered through celite and the filtrate was concentrated under
vacuum to afford the title product (0.42 gm) .
Intermediate-x: Synthesis of Nl-(oxetan-3-yl)benzene-l ,3-diamine
Step-1 : Synthesis of N-(3-nitrophenyl)oxetan-3-amine
To a solution of 3-nitroaniline (2 g, 14.48 mmol) in methanol (30 ml) , were added
oxetan-3-one (1.565 g, 2 1.72 mmol) and zinc chloride (7.89 g, 57.9 mmofjand the
reaction mixture was cooled with an ice bath. Sodium cyanoborohydride (2.73 g,
43.4 mmol) was added to the reaction mixture and the mixture was stirred at room
temperature for 5 hrs. The reaction mixture was poured over cold aq. satd.
ammonium chloride solution and extracted with ethyl acetate (3X75ml). Combined
organic layer was dried over sodium sulfate and concentrated under vacuum. The
crude residue was adsorbed purified by column chromatography using 0- 50%
ethyl acetate in hexanes as eluent to afford the title product (1.2 g) .
Step-2: Synthesis of Nl-(oxetan-3-yl)benzene-l ,3-diamine
N-(3-Nitrophenyl)oxetan-3-amine (0.55g, 2.83 mmol) was taken in methanol (10 ml)
and at 0°C, Pd-C (10%, 0.1 g) was added. The reaction mixture was stirred under
hydrogen atmosphere at room temperature for 5 hrs. The reaction mixture was
filtered through celite and the filtrate was concentrated under vacuum to afford the
title product (0.4 gm).
HNMR (400 MHz, CDC13): d 6.98 (t, 1H, J=8Hz) , 6.16-6. 13 (m, 1H), 5.98-5.95 (m,
1H), 5.84 (t, 1H, J= 2.4 Hz), 4.99 (t, 2H, J= 6.4 Hz) , 4.65-4.57 (m, 1H), 5.27 (t, 2H,
J= 6 Hz) , 4.05 (d, 1H, J= 6.4 Hz) , 3.50 (bs, 2H).
Intermediate-xi: Synthesis of 3-((oxetan-3-yloxy)methyl)aniline
Step-1: Synthesis of l-(chloromethyl)-3-nitrobenzene
(3-Nitrophenyl)methanol (1 g, 6.53 mmol) was dissolved in DCM (8 ml) and cooled
to 0°C followed by addition of thionyl chloride (2.15 ml, 19.59 mmol). The Reaction
mixture was stirred for 4 hrs at room temperature and concentrated under
vacuum, saturated solution of sodium bicarbonate was added to the residue. The
mixture was extracted with ethyl acetate (3X 75ml), combined organic layer was
dried over sodium sulfate and concentrated under vacuum to afford the title
compound (0.9 gm).
Step-2: Synthesis of 3-((3-nitrobenzyl)oxy)oxetane
A mixture of oxetan-3-ol (0.432 g, 5.83 mmol), K2C0 3 (1.61 1 g, 11.66 mmol) and 1-
(chloromethyl)-3-nitrobenzene (1 g, 5.83 mmol) in DMF (10 ml) was heated at 80°C
for 18 hrs. The reaction mixture was cooled to room temperature, water was added
and the mixture was extracted with ethyl acetate (3X 75ml). Combined organic
layer was dried over sodium sulphate and concentrated under vacuum to afford the
crude product which was purified by column chromatography to afford the title
product (0.8 g).
Step-3: Synthesis of 3-((oxetan-3-yloxy)methyl)aniline
3-((3-Nitrobenzyl)oxy)oxetane (0.3g, 1.434 mmol) was taken in methanol (10 ml)
and at 0°C, Pd-C (10%, 0.07 g) was added. The reaction mixture was stirred under
hydrogen atmosphere at room temperature for 5 hrs. The reaction mixture was
filtered through celite and the filtrate was concentrated under vacuum to afford the
title product (0.2 gm).
HNMR (400 MHz, CDC13) : d 7. 12 (t, 1H, J= 8 Hz) 6.70-6.52 (m, 3H), 4.60 (bs, 2H),
4.37-4.12 (m, 5H), 3.73 (brs, 2H).
Intermediate xii: (3-aminophenyl)(cyclopropyl)methanone
Intermediate xiv was prepared using procedure depicted in the reference "Journal
of Medicinal Chemistry, 1995, Vol. 38, #18, page 3624-3637".
Examples
The following examples demonstrate preparation of few representative compounds
embodied in formula (I), however, the same should not be construed as limiting the
scope of the invention.
Example-1: Synthesis of l-(3-(cyclopropylsulfonyl)phenyl)-5-((2-fluoro-4-iodo
phenyl)amino)-6,8-dimethylpyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione
(Compound 1)
Step-1: Synthesis of l-(2-fluoro-4-iodophenyl)-3-(4-methoxybenzyl)-6,8-dimethyl-
2,4,7-trioxo- 1,2, 3,4,7, 8-hexahydropyrido[2,3-d]pyrimidin-5-yl 4-
methylbenzenesulfonate (lb)
Under nitrogen atmosphere, to a solution of l-(2-fluoro-4-iodophenyl)-5-hydroxy-3-
(4-methoxybenzyl)-6,8-dimethylpyrido[2,3-d]pyrimidine-2,4,7(lH,3H,8H)-trione (la)
(41g, 72.8mmol) (Prepared as per reference WO2005121 142) in acetonitrile (300
ml), triethylamine (30.4 ml, 218 mmol) and trimethylamine hydrochloride (3.48 g,
36.4 mmol) was added drop-wise p-toluensulfonylchloride (27.8 g, 146 mmol) in
acetonitrile (300 ml) at 0 °C, and the mixture was stirred under Ice cooling for lhr,
and at room temperature for 24 h . To the reaction mixture was added methanol
(220 ml), and the mixture was stirred at room temperature for lh. The precipitated
crystals were collected by filtration, dried under vacuum to afford the titled
compound (40.5 g, 78%)
H NMR(400 MHz,DMSO-d6) 8: 7.95 (dd, J = 1.6 and 9.6 Hz, 1H), 7.84 (d, J =8.4
Hz, 2H), 7.72 (dd, J = 1.2 and 8.4 Hz, 2H), 7.46 (d, J = 8.4 Hz, 2H), 7.35 (t, J =8.4
Hz, IH), 7.23 (d, J =8.8 Hz, 2H), 6.86 (d, J =8.8 Hz, 2H), 4.92 (d, J = 16 Hz, IH),
4.77 (d, J = 16 Hz, IH), 3.71 (s, 3H), 2.76 (s, 3H), 2.42 (s, 3H), 1.53 (s, 3H).
MS: m/z : 717.9
Step-2: Synthesis of 5-((3-(cyclopropylsulfonyl)phenyl)amlno)-l-(2-fluoro-4-
lodophenyl)-3-(4-methoxybenzyl)-6,8-dlmethylpyrldo[2,3-d]pyrlmldlne-
2,4,7(lH,3H,8H)-trione (lc)
A mixture of l-(2-fluoro-4-lodophenyl)-3-(4-methoxybenzyl)-6,8-dlmethyl-2,4,7-
trloxo- 1,2,3,4,7,8-hexahydropyrido[2,3-d]pyrlmldln-5-yl 4-methylbenzenesulfonate
(lb) (1.0 g, 1.394 mmol), 3-(cyclopropylsulfonyl)anlllne (lntermedlate-1) (1.237 g,
6.27 mmol) and 2,6-LUTIDINE (0.487 ml, 4.18 mmol) n N,N-Dlmethylacetamlde (
0.5 ml) was heated at 140° C for 18h n sealed vial. After cooling to rt, the reaction
mixture was poured over water (100 ml) and resulting solid was filtered. Residue
was purified by flash chromatogrhaphy on a comblflash Instrument to obtain the
product.
HNMR (400 MHz, DMSO- ) 8 (ppm): 10.22 (s, IH), 7.97 (dd, J=9.2 and 1.6 Hz,
IH), 7.74 (dd, J=8 and 1.2Hz, IH), 7.56 (t, J=7.6 Hz, IH), 7.47 (d, J= 8.0Hz, IH),
7.38 (t, J=8 Hz, IH), 7.27-7.33 (m, 4H), 6.83-6.87 (m, 2H), 4.94-5.04 (m, 2H), 3.69
(s, 3H), 2.84 - 2.87 (m, IH), 2.78 (s, 3H), 1.55 (s, 3H), 1.02-1.04 (m, 2H), 1.05-1.09
(m, 2H)
MS: m/z : 743.1 [M+l]
Step-3: Synthesis of synthesis of l-(3-(cyclopropylsulfonyl)phenyl)-5-((2-fluoro-4-
iodophenyl)amino)-3-(4-methoxybenzyl)-6,8-dimethylpyrido[4,3-d]pyrimidine-
2,4,7(lH,3H,6H)-trione (Id)
Sodium methoxide (25% solution in MeOH) (0.2 ml) was added to a solution of 5-
((3-(cyclopropylsulfonyl)phenyl)amino)-l-(2-fluoro-4-iodophenyl)-3-(4-
metho^benzyl)-6,8-dimethylpyrido[2,3-d]pyrimidine-2,4,7(lH,3H,8H)-trione (lc)
(0.4 g, 0.539 mmol) in THF (3.5 ml). Resulting solution was stirred unde 2 atm for
1 h , and quenched by addition of dil. HC1. Solvents were evaporated in vacu o and
residue was triturated in water. Solid product was filtered and dried under vacuum
and used as such for the next step.
Ή NMR (400 MHz, DMSO- ) d (ppm): 11.09 (s, 1H), 7.88 (brs, 2H), 7.71 (brs, 3H),
7.15 (brs, 3H), 6.81 to 6.83 (d, J=7.2Hz, 3H ), 4.87 (brs, 2H), 3.70 (s, 3H), 3.32 (brs,
3H), 2.88-2.89 (m, 1H), 1.14 (s, 3H), 1.10-1.1 1 (m, 2H), 1.03-1.05 (d, J= 7.4Hz, 2H)
MS: / z: 743.1 [M+l].
Step-4: Synthesis of synthesis of l-(3-(cyclopropylsulfonyl)phenyl)-5-((2-fluoro-4-
iodophenyl)amino)-6,8-dimethylpyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (1)
Aluminium chloride (0.628 g, 4.71 mmol) was added to a solution of l-(3-
(cyclopropylsulfonyl)phenyl)-5-((2-fluoro-4-iodophenyl)amino)-3-(4-methoxybenzyl)-
6,8-dimethylpyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Id) (0.350 g, 0.471
mmol) in Anisole (2 ml) . Resulting mixture was stirred under 2 atm for 18 h at RT.
Reaction was quenched by addition of MeOH. Solvents were evaporated in vacuo.
Residue was acidified using dil. HC1. Resulting solid was filtered and heated to
reflux in 2-propanol (20 ml) for 1 hr. Reaction mixture was brought to RT and
filtered. Residue was purified by flash chromatography to give the pure product.
HNMR (400 MHz, DMSO-d6), d 11.66 (s, 1H), 11.20 (s, 1H), 7.93-7.83 (m, 2H),
7.83-7.73 (m, 3H), 7.56 (dd, J = 1.2 and 8.4 Hz, 1H), 6.97 (t, J = 8.8 Hz, 1H), 3.07
(s, 3H), 2.91-2.87 (m, 1H), 1.16 (s, 3H), 1.15-1.1 1 (m, 2H), 1.06-1.04 (m, 2H).
Example-2: Synthesis of 3-cyclopropyl-l-(2-fluoro-4-iodophenyl)-5-((3-(3-
hydroxyazetidin- 1-yl)phenyl)amino)-6,8-dimethylpy rido[2 ,3-d]pyrimidine-
2,4,7(lH,3H,8H)-trione. (Compound 2)
Step-1: Synthesis of 3-cyclopropyl-l-(2-fluoro-4-iodophenyl)-5-((3-(3-
hydroxyazetidin- 1-yl)phenyl)amino)-6,8-dimethylpyrido [2 ,3- ]pyrimidine-
2,4,7(lH,3H,8 H)-trione (2b)
3-cyclopropyl- l-(2-fluoro-4-iodophenyl)-6,8-dimethyl-2,4,7-trioxo- 1,2,3,4,7,8-
hexahydropyrido[2,3-d]pyrimidin-5-yl 4-methylbenzenesulfonate (2a) (0.5 g, 0.78
mmol), l-(3-aminophenyl)azetidin-3-ol (vi) (0.13 g, 0.784 mmol) in DMA (1.5 ml),
and 2,6-lutidine (0.33 ml, 2.86 mmol) was added in the sealed tube and heated at
130°C for 10 hr under nitrogen atmosphere. After completion of the reaction, the
reaction mixture was poured into ice cold water and extracted with ethyl acetate (2
x 10 ml). The organic layers were combined, washed with saturated ammonium
chloride, water and dried over sodium sulfate. The organic layer was then
concentrated under reduced pressure to obtain a crude product which was purified
by column chromatography to yield the titled compound (2b) as a white solid (0.09
g, 18 %) [MS: m/z = 630 (M+l)].
Step-2: Synthesis of 3-cyclopropyl-5-((2-fluoro-4-iodophenyl)amino)-l-(3-(3-
hydroxyazetidin-l-yl)phenyl)-6,8-dimethylpyrido[4,3-d]pyrimidine-2,4,7(lH,3H,
6H)-trione (2)
3-cyclopropyl-l-(2-fluoro-4-iodophenyl)-5-((3-(3-hydroxyazetidin-lyl)
phenyl)amino)-6,8-dimethylpyrido[2^ (2b)
(0.03 g, 0.048 mmol) was taken in tetrahydrofuran (1 ml) at room temperature,
sodium methoxide (30% in MeOH, 23 uL) was added and the reaction mixture was
stirred at the same temperature for 2 hr under nitrogen atmosphere. The progress
of the reaction was monitored on HPLC. After complete consumption of the
substrate, the reaction mixture was concentrated and submitted as such for LCMS
and preparative HPLC to yield the titled compound (2) as white solid (0.013 g)
HNMR (400 MHz, DMSO-d6), 8 11.09 (s, IH), 7.75 (bs, IH), 7.53-7.51 (m, IH),
7.20 (t, IH), 6.90 (t, IH), 6.64 (d, IH, J=8Hz), 6.43 (d, 2H, J=2Hz), 5.63 (d,
lH,J=6.4Hz), 4.56 (bs, IH), 4.07-4.04 (m, 2H), 3.50-3.47 (m, 2H), 3.07 (s, 3H), 1.29
(s, 3H), 1.09 (t, lH,J=6.8Hz), 1.0-0.9 (m, 2H), 0.64-0.62 (m, 2H). MS: m/z = 630
(M+l)].
The compounds given below in Table 1: were prepared by procedure similar to the
one described above in Example 2 with the above stated intermediates with
appropriate variations in reactants, reaction conditions and quantities of reagents.
Table-1:
Compoun Intermedia IUPAC name Analytical data
d No. te No.
3 i 3-cyclopropyl- 1-(3- iHNMR (400 MHz, DMSO-d6), d
(cyclopropylsulfonyl) 11.06 (s, IH), 7.91-7.90 (m, 2H),
phenyl)-5-((2-fluoro-
4-iodophenyl)amino)- 7.81-7.73 (m, 3H), 7.57-7.54 (m,
6,8- IH), 6.96 (t, J = 8.4 Hz, IH), 3.09
dimethylpyrido [4,3- (s, 3H), 2.91-2.87 (m, IH), 2.64-
d]pyrimidine-
2,4,7(1H,3H,6H)- 2.60 (m, IH), 1.16 (s, 3H), 1.13-
trione 0.86 (m, 6H), 0.69-0.67 (m, 2H).
MS: m/z 662.9 (M+l).
vii 3-cyclopropyl-5-((2- HNMR (400 MHz, DMSO-d6), d
iluoro-4- 11.10(s, IH), 7.9 (dd, IH ,J= 2 and
iodophenyl)amino)-
6,8-dimethyl-l-(3-(2- 8.4 Hz ), 7.50 (dd, IH, J = 2 and
oxoazetidin- 1- 8.4 Hz), 7.43-7.41 (m, 2H), 7.33
yl)phenyl)pyrido[4,3- (d, IH, J = 6.0 Hz), 7.07 (d, IH,
d]pyrimidine-
J=6.0 Hz), 6.92 (t, IH, J=8.6 Hz),
2,4,7(1H,3H,6H)-
trione 3.65 (t, 2H, J = 4.4 Hz), 3.10-3.07
(m, 5H), 2.63-2.59 (m, IH), 1.25
(s, 3H), 0.96-0.94 (m, 2H), 0.68-
0.66 (m, 2H).
MS: m/z 628 (M+l).
X 3-cyclopropyl-5-((2- iHNMR (400 MHz, DMSO-d6), d
iluoro-4- 11.09 (s, IH), 7.79 (d, IH, J = 10.4
iodophenyl)amino)-
6,8-dimethyl-l-(3- Hz), 7.55 (d, IH, J=8.8 Hz), 7.24
(oxetan-3- (m, IH), 6.90 (t, IH, J = 8.8 Hz),
ylamino)phenyl)pyrid 6.58-6.56 (m, 2H), 6.51-6.50 (m,
o[4,3-d]pyrimidine-
IH), 6.47-6.45 (m, IH), 4.82 (t, 2,4,7(1H,3H,6H)-
trione 2H, J = 6.4 Hz), 4.55-4.50 (m, IH),
4.38 (bs, 2H), 3.07 ( s , 3H), 2.63-
2.55 (m, IH), 1.3 (s, 3H), 0.95-
0.94 (m, 2H), 0.66-0.64 (bs, 2H).
MS: m/z 630 (M+l).
V tert-butyl 3-((3-(3- MS: m/z 757 (M+l).
cyclopropyl-5-((2-
iluoro-4-
iodophenyl)amino)-
6,8-dimethyl-2,4,7-
trioxo-3,4,6,7-
tetrahydropyrido[4,3-
d]pyrimidin- 1(2H)-
yl)phenyl)carbamoyl)
azetidine- 1-
carboxylate
viii 3-cyclopropyl -5-((2- HNMR (400 MHz, DMSO-d6), 8
iluoro-4- 11.06 (s, 1H), 7.78 (d, 1H, J = 10.8
iodophenyl)amino)-
6,8-dimethyl-l -(3- Hz), 7.54 (d, 1H, J=8.0 Hz), 7.36
(oxetan-3- (t, 1H, J=8.0 Hz), 7.99 (d, 1H,
yloxy)phenyl)pyrido[4 J=8.0 Hz), 6.95-6.87 (m, 2H), 6.80
,3-d]pyrimidine-
2,4,7(1H,3H,6H)- (dd, 1H, J = 2.4 and 6.0 Hz), 5.30-
trione 5.27 (m, 1H), 4.91 (t, 2H, J = 6.6
Hz), 4.52 (t, 2H, J=5.8 Hz), 3.08 (s,
3H), 2.61 (bs, 1H), 1.23 (s, 3H),
0.96-0.94 (m, 2H), 0.67-0.65 (m,
2H).
MS: m/z 631 (M+l).
iv l - (3-(azetidin-l- MS: m/z 614 (M+l).
yl)phenyl)-3-
cyclopropyl-5-((2-
iluoro-4-
iodophenyl)amino)-
6,8-
dimethylpyrido[4,3-
d]pyrimidine-
2,4,7(1H,3H,6H)-
trione
iii 3-cyclopropyl -5-((2- HNMR (400 MHz, DMSO-d6), 8
iluoro-4- 11.33 (s, 1H), 7.70 (d, 1H, J = 8
iodophenyl)amino)- 1-
(3- (3-hydroxyoxetan- Hz), 7.55-7.46 (m, 3H), 7.31-7.30
3-yl)phenyl )-6,8- (m, 1H), 6.74-6.60 (m, 2H), 4.94-
dimethylpyrido[4,3-
d]pyrimidine- 4.86 (m, 4H), 3.21 (s, 3H), 2.78-
2,4,7(1H,3H,6H)- 2.72 (m, 2H), 1.36 (s, 3H), 1.15-
trione
1.13 (m, 2H), 0.83-0.81 (m, 2H).
MS: m/z 631 (M+l).
ii N- (3- (3-cy clo ro l HNMR (400 MHz, DMSO-d6), 8
s ((2-fluoro -4- 11.05 (s, IH), 9.94 (s, IH), 7.78 (d,
iodophenyl) amino)-
6 ,8-dimethyl -2,4 ,7- IH, J=9.6Hz), 7.67 (d, IH, J=8Hz),
trioxo-3,4,6,7- 7.61 (s, IH), 7.54 (d, IH, J=8.4Hz),
tetrahydropyrido [4,3- 7.38 (t, IH, J=7.2Hz), 7.07 (d, IH,
d]pyrimidin- 1(2H)-
J=7.2Hz), 6.91 (t, IH, J=8.4Hz),
yl)phenyl )-3-
methyloxetane -3- 4.82 (d, 2H, J=6Hz), 4.33 (d, 2H,
carboxamide J=6Hz), 3.07 (s, 3H), 2.60 (m, IH),
1.60 (s, 3H), 1.27 (s, 3H), 0.97-
0.94 (m, 2H), 0.66-0.64 (s, 2H).
MS: m/z 672 (M+l).
xli l -(3- iHNMR (400 MHz, DMSO-d6), 8
(cyclopropanecarbon 11.08 (s,lH), 8.12-8.09 (m, IH),
yl)phenyl )-3-
cyclopropyl -5-((2- 8.01 (bs, IH), 7.79 (dd, IH, J=2 Hz
iluoro -4- J = 10.4Hz), 7.69-7.62 (m, 2H),
iodophenyl) amino)- 7.56-7.54 (m,lH), 6.94 (t, IH
6,8-
J=8.4Hz), 0.68 (bs, 2H), 3.07 (s, dimethylpyrido [4,3-
d]pyrimidine- 3H), 2.90 (m, IH), 2.61-2.60 (m,
2,4,7(1H,3H,6H)- IH), 1.18 (s, 3H), 1.06-1.08 (m,
trione 4H), 0.96-0.95 (m, 2H).
MS: m/z 627.1 (M+l).
i l -(3- iHNMR (400 MHz, DMSO-d6), 8
(cyclopropylsulfonyl) 11.18 (s, IH), 7.94-7.92 (m, 2H),
phenyl )-5-((2-fluoro-
4-iodophenyl)amino)- 7.84-7.45 (m, 3H), 6.96 (t, J = 8.8
3,6,8- Hz, IH), 3.22 (s, 3H), 3.09 (s, 3H),
trimethylpyrido [4,3- 1.78-1.74 (m, IH), 1.18 (s, 3H),
d]pyrimidine-
2,4,7(1H,3H,6H)- 1.13-1.12 (m, 2H), 1.07-1.05 (m,
trione 2H).
MS: m/z 637 (M+l).
x 5-((2-fluoro-4- HNMR (400 MHz, DMSO- 6), d
iodophenyl)amino)- 11.20 (s, IH), 7.79 (dd, IH, J = 2
3,6,8-trimethyl-l-(3-
((oxetan-3- and 8 Hz), 7.55 (d, IH, J = 8.4 Hz),
yloxy)methyl)phenyl) 7.47-7.43 (m, IH), 7.39-7.33 (m,
pyrido[4,3- 3H), 6.93 (t, IH, J=8.4 Hz), 4.63-
d]pyrimidine-
2,4,7(1H,3H,6H)- 4.60 (m, 3H), 4.69 (s, 2H), 4.42-
trione 4.41 (m, 2H), 3.21 (s, 3H), 3.08 (s,
3H), 1.19 (s, 3H).
MS: m/z 619 (M+l).
iii 5-((2-fluoro-4- HNMR (400 MHz, DMSO-d6), d
iodophenyl)amino)-1- 11.22 (s, IH), 7.79 (dd, IH, J = 10
(3- (3-hydroxyoxetan3-
yl)phenyl)-3,6,8- and 1.6 Hz), 7.66 (d, IH, J = 8 Hz),
trimethylpyrido [4,3- 7.58-7.48 (m, 3H), 7.36 (dd, IH,
d]pyrimidine- J = 8.8 and 1.2 Hz), 6.93 (t, IH, J =
2,4,7(1H,3H,6H)-
8.8 Hz), 4.78 (d, 2H, J = 6.4 Hz), trione
4.65 (bs, 2H), 3.20 (s, 3H), 2.60
(bs, IH), 3.08 (s, 3H), 1.19 (s, 3H).
MS: m/z 605 (M+l).
viii 5-((2-fluoro-4- iHNMR (400 MHz, DMSO-d6), d
iodophenyl)amino)- 11.19 (s, IH), 7.79 (dd, IH, J = 2
3,6,8-trimethyl-l-(3-
(oxetan-3- and 8.4 Hz), 7.55 (d, IH, J = 8.4
yloxy)phenyl)pyrido[4 Hz), 7.37 (t, IH, J = 8.0 Hz), 7.02
,3-d]pyrimidine- (dd, IH, J = 1.2 and 6.8 Hz), 6.96-
2,4,7(1H,3H,6H)- 6.91 (m, 2H), 6.86 (dd, 1H, J= 2
trione and 8.4 Hz), 5.32-5.27 (m, 1H),
4.91 (t, 2H, J= 6.8 Hz), 4.52 (t, 2H,
J=6.0 Hz), 3.21 (s, 3H), 3.08 (s,
3H), 1.24 (s, 3H).
MS: m/z 604 (M+l).
iv l - (3-(azetidin-l- HNMR (400 MHz, CDC13), 8 11.45
yl)phenyl)-5-((2- (s, 1H), 7.53 (dd, 1H, J=2 and 10
iluoro-4-
iodophenyl)amino)- Hz), 7.45( d, 1H, J= 8.4), 7.22 (t,
3,6,8- 1H, J=8 Hz), 6.70-6.63 (m, 3H),
trimethylpyrido[4,3- 6.56-6.55 (m, 1H), 3.66 (t, 2H, J=
d]pyrimidine-
6.4 Hz), 3.39 (s, 3H), 3.34 (t, 2,4,7(1H,3H,6H)-
trione 2H,J=6.8Hz), 3.23(s, 3H), 2.10-
2.04 (m, 2H), 1.5 (s, 3H).
MS: m/z 588 (M+l).
X 5-((2-fluoro-4- iHNMR (400 MHz, DMSO-d6), 8
iodophenyl)amino)- 11.22 (s, 1H), 7.79 (dd, 1H, J= 2
3,6,8-trimethyl-l -(3-
(oxetan-3- and 12 Hz), 7.55 (d, 1H, J=8.4),
ylamino)phenyl)pyrid 7.15 (t, 1H, J=8 Hz), 6.91 (t, 1H,
o[4 ,3-d]pyrimidine- J= 8.8 Hz), 6.59 (d, 2H, J= 6.8),
2,4,7(1H,3H,6H)-
6.49 (d, 2H, J= 8Hz), 4.82 (t, 2H, trione
J=6.4), 4.54-4.50 (m, 1H), 4.40-
4.37 (m, 2H), 3.2 (s, 3H), 3.07(s,
3H), 1.32 (s, 3H).
MS: m/z 604 (M+l).
ii N-(3-(5-((2-fluoro-4- iHNMR (400 MHz, DMSO-d6), d
iodophenyl)amino)- 11.18 (s, 1H), 9.93 (s, 1H), 7.78 (d,
3,6,8-trimethyl-
2,4,7 -trioxo-3,4,6,7- 1H, J=8.8Hz) 7.67 (s, 1H), 7.64 (d,
tetrahydropyrido [4,3- 1H, J=8 Hz), 7.54 (d, 1H,
d]pyrimidin- 1(2H)- J=8.8Hz), 7.40 (t, 1H, J=8Hz), 7.1 1
yl)phenyl)-3-
methyloxetane-3- (dd, 1H, J=1.2 and 8Hz), 6.93 (t,
carboxamide 1H, J=8.8Hz), 4.82 (d, 2H, J=6Hz),
4.33 (d, 2H, J=6Hz), 3.2 (s, 3H),
3.08 (s, 3H), 1.60 (s, 3H), 1.28 (s,
3H).
MS: m/z 646 (M+l).
19 Ix 2-((l-(3-(5-((2-fluoro- HNMR (400 MHz, DMSO-d6), d
4-iodophenyl)amino)- 11.24 (s, 1H), 7.77 (d, 1H, J = 9.6
3,6,8-trimethyl-
2,4,7-trioxo-3,4,6,7- Hz), 7.53 (d, 1H, J=8 Hz), 7.33 (s,
tetrahydropyrido [4,3- 1H), 7.26-7.21 (m, 2H), 6.91(t,
d]pyrimidin- 1(2H)- 1H, J = 8.4 Hz), 6.68 (d, 1H, J=8.4
yl)phenyl) azetidin- 3-
), 6.48- 6.46 (m, 2H), 4.47 (m, 1H),
yl)oxy)acetamide
4.06-4.02 (m, 2H), 3.82 (s, 2H),
3.68-3.65 (m, 2H), 3.19 ( s , 3H),
3.08 (s, 3H), 1.31 (s, 3H).
MS: m/z 661 (M+l).
Example 3 : Synthesis of 5-((2-fluoro-4-iodophenyl)amino)-l-(3-(3-
fluorooxetan-3-yl)phenyl)-3,6,8-trimethylpyrido[4,3-d]pyrimidine-
2,4,7(lH,3H,6H)-trione (Compound 20).
To a solution of 5-((2-Fluoro-4-iodophenyl)amino)-l-(3-(3-hydroxyoxetan-3-
yl)phenyl)-3,6,8-trimethylpyrido[4,3-d]pyrimidine-2,4J(lH,3H,6H)-trione
(compound 14) (0.02 g, 0.033 mmol) in DCM (7 ml), DAST (0.017ml, 0.132 mmol)
was added at -78 °C, the reaction mixture was stirred at room temperature for
lhr. Reaction mixture was concentrated and crude compound was purified by
column chromatography over silica gel using ethyl acetate (40%) in hexane as
eluent. Obtained solid was triturated in diethyl ether to afford the titled compound
(0.012 g).
HNMR (400 MHz, DMSO-d6), d 11.21 (s, 1H), 7.79 (dd, 1H, J= 2.0 and 8.4 Hz),
7.63-7.59 (m, 3H), 7.55 (d, 1H, J=10) 7.49 (d, 1H, J= 7.6 Hz), 6.94 (t, 1H, J=
8.8Hz), 5.02-4.87 (m, 4H), 3.21 (s, 3H), 3.08 (s, 3H), 1.19 (s, 3H). ESI-MS: [m/z =
607 (M+l)].
Example 4 : Synthesis of N-(3-(3-cyclopropyl-5-((2-fluoro-4-iodophenyl)amino)-
6,8-dimethyl-2,4,7-trioxo-3,4,6,7-tetrahydropyrido[4,3-d]pyrimidin-l(2H)-
yl)phenyl)azetidine-3-carboxamide (21).
To a solution of tert-butyl 3-((3-(3-cyclopropyl-5-((2-fluoro-4-iodophenyl)amino)-
6,8-dimethyl-2,4,7-trioxo-3,4,6,7-tetrahydropyri
carbamoyl)azetidine-l-carboxylate (compound 6) (0.075 g, 0.099 mmol) in DCM
(5.0 ml), TFA (0.038 ml, 0.496 mmol) was added at 0 °C. Reaction mixture was
stirred at room temperature for 15 hrs, treated with satd. aq. aHCO and the
resulting mixture was extracted with DCM (3 x 10 ml). The combined organic layers
were washed with water, brine and dried over sodium sulfate. The organic layer
was concentrated under vacuum to obtain a crude product wchich was triturated
in diethyl ether to afford titled compound (0.03 g).
HNMR (400 MHz, DMSO-d6), d 11.07 (s, 1H), 10.32 (s, 1H), 8.67 (bs, 1H), 7.81-
7.80 (dd, 1H, J= 10.4 and 1.6 Hz), 7.65-7.63 (m, 1H), 7.57-7.55 (d, 1H), 7.44-7.39
(m, 1H), 7.09-7.07 (d, 1H, J= 6.4 Hz), 6.94-6.90 (t, 1H, J= 8.8 Hz), 4.81-4.79 (d,
1H, J= 8.8 Hz), 4.10-4.09 (m, 1H), 3.76-3.72 (m, 1H), 3.07 (s, 3H), 2.67-2.60 (m,
2H), 2.33-2.27 (m, 2H), 1.25 (s, 3H), 0.66 (bs, 2H), 0.96-0.94 (m, 2H). ESI-MS: [m/z
= 657 (M+l)]
PHARMACOLOGICAL ACTIVITY:
Protocol for In-Vitro Experiments:
Example-A: Identification of compounds inhibiting MEK kinase activity
In a 25 mI reaction, MEK enzyme (final concentration 2-4 m / ml , and ERK
substrate (final concentration 50-100 m / ml , were incubated with various
concentration of test compounds (diluted such that the reaction had 1% DMSO), at
25-30°C for 20 to 120 min on a shaker incubator. The reactions were initiated by
the addition of ATP. The reactions were terminated by adding an equal volume of
KinaseGlo reagent (Promega), following the manufacturer's instructions. The plates
were read on a luminometer. IC50 calculations were done using GraphPad Prism 5.
The Compounds of the invention exhibited IC50 values ranging between 1 nM to
600nM in MEK inhibition assay.
Compound No's 1, 5, 7, 10, 11, 12, 13, 14, 15, 16, 18 and 19 exhibited IC50 values
in the range 1 to 600 nM.
Example-B: Analysis of ERK phosphorylation
This assay was carried out with human melanoma cells, human and mouse colon
cancer cells. Cells were treated for l h with various concentrations of test
compounds. ERK phosphorylation analysis was performed using the Alphascreen
SureFire Phospho-ERK 1/2 Kit (Perkin Elmer), by following the manufacturer's
instructions. % inhibition of ERK phosphorylation was determined as:
100 - {(RFU test - RFU lysis buffer control) / (RFU vehicle treated control - RFU
lysis buffer control)} x 100.
The compounds prepared were tested using the above assay procedure and the
results obtained are given in Table 2. Percentage inhibition at concentrations of
pERK at IOOhM, IOhM, InM for the stated examples is setworth here. The
percentage inhibiton at the above depicted concentrations for the compounds
stated are given in the following groups.
Group-A: Compounds having 50-100% inhibition at InM.
Group-B: Compounds having 50-100% inhibition at IOhM
Group-C: Compounds having 50-100% inhibition at lOOnM.
Table-2:
Group Compounds
A 1, 8, 11, 12, 14, 15, and 17
B 2, 4, 5, 10, 13, 18, 19 and 20
C 3, 7, 9, 16 and 2 1
CLAIMS
1. A compound of the general formula I, its tautomeric forms, its
pharmaceutically acceptable salts, their combinations with suitable medicament
and pharmaceutical compositions containing them,
wherein,
R1 is selected from the group consisting of hydrogen, substituted- or
unsubstituted-alkyl, substituted- or unsubstituted- cycloalkyl, and substituted- or
unsubstituted- heterocyclyl;
R is selected from the group consisting of -R -E, -S0 2R7, and -C(0)R8;
R3 is selected from the group consisting of hydrogen, substituted- or
unsubstituted- alkyl, and substituted- or unsubstituted- cycloalkyl;
R4 is selected from the group consisting of hydrogen, halogen, substituted- or
unsubstituted-alkyl, and substituted- or unsubstituted- cycloalkyl;
R5 is substituted- or unsubstituted- aryl, wherein the substituents are selected
from with Ra and Rb;
Ra and Rb are selected from the group consisting of hydrogen, halogen and
haloalkyl;
R6 is selected from the group consisting of direct bond, -[C(R )Rd]nNR9 , -
[C(Rc)Rd]nO-, -NHC(=0)[C(Rc)Rd]p , -S(0) 2NH-, -NHC(=0)[CR (Rd)]NR9-, -
NHC(=0)[CR (R )]0-, and -NHS(0) - ;
Rc and Rd are each independently selected from the group consisting of hydrogen
and substituted- or unsubstituted- alkyl;
E is substituted- or unsubstituted-four membered heterocyclic ring, wherein
the substituents are selected from the group consisting of alkyl, halogen, -
C(=0)OR , and -OR ;
Re is selected from the group consisting of hydrogen, substituted- or
unsubstituted-alkyl, and substituted or unsubstituted cycloalkyl;
R7 is selected from the group consisting of substituted- or unsubstitutedcycloalkyl,
and substituted- or unsubstituted- cycloalkenyl;
R8 is selected from the group consisting of substituted- or unsubstitutedalkyl,
substituted- or unsubstituted- alkenyl, substituted- or unsubstitutedalkynyl,
substituted- or unsubstituted- cycloalkyl, and substituted- or
unsubstituted- cycloalkenyl;
R9 is selected from the group consisting of hydrogen, substituted- or unsubstitutedalkyl,
substituted- or unsubstituted- alkenyl, substituted- or unsubstitutedalkynyl,
substituted- or unsubstituted- cycloalkyl and substitutedor
unsubstituted- cycloalkenyl;
n is an integer selected from the group consisting of 0, 1 and 2 ;
p is an integer selected from the group consisting of 0 and 1.
when the alkyl group and alkenyl group is substituted, the alkyl group and alkenyl
group is substituted with 1 to 4 substituents independently selected from the
group consisting of oxo, halogen, nitro, cyano, perhaloalkyl, cycloalkyl, aryl,
heteroaryl, heterocyclyl, -OR10b , -SO2R10a , -C(=O)OR10a , -OC(=O)R 0 , -C(=0)N(H)R10 ,
.QRioa, -N(H)Rio, -N(alkyl)Rio
-N(H)C(=0)N(alkyl)R , -NH-S0 2-alkyl and -NH-S0 2-cycloalkyl;
when the cycloalkyl group and cycloalkenyl group is a substituted, the cycloalkyl
group and cycloalkenyl group is substituted with 1 to 3 substituents independently
selected from the group consisting of oxo, halogen, nitro, cyano, alkyl, alkenyl,
perhaloalkyl, aryl, heteroaryl, heterocyclyl, -OR10b , -SO2R10a, -C(=O)R10a , -
N(H)R10 , -N(alkyl)R10 , -N(H)C(=0)N(H)R10 , and -N(H)C(=0)N(alkyl)R10 , -NH-S0 2-alkyl
and -NH-S0 2-cycloalkyl;
when the aryl group is a substituted, the aryl group is substituted with 1 to 3
substituents independently selected from the group consisting of halogen, nitro,
cyano, hydroxy, alkyl, alkenyl, perhaloalkyl, cycloalkyl, cycloalkenyl, heterocycle, -
O-alkyl, -O-perhaloalkyl, -N(alkyl)alkyl, -N(H)alkyl, -NH2, -S0 2-alkyl, -S0 2-
perhaloalkyl, -N(alkyl)C(=0)alkyl, -N(H)C(=0)alkyl, -C(=0)N(alkyl)alkyl,
C(=0)N(H)alkyl, -C(=0)NH2, -S0 2N(alkyl)alkyl, -S0 2N(H)alkyl, -S0 2NH2, -NH-S0 2-
alkyl and -NH-S0 2-cycloalkyl;
when the heteroaryl group is a substituted, the heteroaryl group is substituted
with 1 to 3 substituents independently selected from the group consisting of
halogen, nitro, cyano, hydroxy, alkyl, alkenyl, perhaloalkyl, cycloalkyl,
cycloalkenyl, heterocycle, -O-alkyl, O-perhaloalkyl, -N(alkyl)alkyl, -N(H)alkyl, -NH2,
-S0 2-alkyl, -S0 2-perhaloalkyl, -N(alkyl)C(=0)alkyl, -N(H)C(=0)alkyl,
C(=0)N(alkyl)alkyl, -C(=0)N(H)alkyl, -C(=0)NH2, -S0 2N(alkyl)alkyl, -S0 2N(H)alkyl, -
S0 2NH2, -NH-S0 2-alkyl and -NH-S0 2-cycloalkyl;
when the heterocyclyl group is a substituted, the heterocyclyl group is substituted
with 1 to 3 substituents, when the heterocyclic group is substituted on a ring
carbon of the 'heterocycle', the substituents are independently selected from the
group consisting of halogen, nitro, cyano, oxo, alkyl, alkenyl, perhaloalkyl,
cycloalkyl, cycloalkenyl, aryl, heteroaryl, heterocyclyl, -OR 10b , -C(=O)OR 10a , -
OC(=O)R 0a , -C (=0)N(H)R , -C(=0)N(alkyl)R ° , -N(H)C(=O)R 0 , -N(H)R °, -N(alkyl)R ,
-N(H)C (=0)N(H)R , -N(H)C(=O)N(alkyl)R ; the substituents on ring nitrogen of the
'heterocycle'; substituents are independently selected from the group consisting of
alkyl, alkenyl, cycloalkyl, cycloalkenyl, aryl, heteroaryl, -SO2R 10a , -C(=O)R 10a ,
C(=O)OR 0a -C (=0)N(H)R , -C (=0)N(alkyl)R °, -NH-S0 2-alkyl and -NH-SO2-
cycloalkyl;
R 10 is selected from the group consisting of hydrogen, alkyl, alkenyl, cycloalkyl,
cycloalkenyl, aryl, heteroaryl, and heterocyclyl;
R i oa
S selected from the group consisting of alkyl, alkenyl, perhaloalkyl, cycloalkyl,
cycloalkenyl, aryl, heteroaryl, and heterocyclyl; and
R i ob s selected from the group consisting of hydrogen, alkyl, alkenyl, perhaloalkyl,
cycloalkyl, cycloalkenyl, aryl, heteroaryl, and heterocyclyl.
2. A compound of the general formula la, its tautomeric forms, its
pharmaceutically acceptable salts, their combinations with suitable medicament
and pharmaceutical compositions containing them,
(la)
wherein,
R1 is selected from the group consisting of hydrogen, substituted- or
unsubstituted-alkyl, substituted- or unsubstituted- cycloalkyl, and substituted- or
unsubstituted- heterocyclyl;
R3 is selected from the group consisting of hydrogen, substituted- or
unsubstituted- alkyl, and substituted- or unsubstituted- cycloalkyl;
R4 is selected from the group consisting of hydrogen, halogen, substituted- or
unsubstituted-alkyl, and substituted- or unsubstituted- cycloalkyl;
Ra and Rb are selected from the group consisting of hydrogen, halogen and
haloalkyl;
R6 is selected from the group consisting of direct bond, -[C(R )Rd]nNR9-, -
[C(Rc)Rd]nO-, -NHC(=0)[C(Rc)Rd]p-, -S (0 )2NH-, -NHC(=0)[CR (R )]NR9-, -
NHC(=0)[CR (R ) ]0 - , and -NHS (0)2- ;
Rc and Rd are each independently selected from the group consisting of hydrogen
and substituted- or unsubstituted- alkyl;
E is substituted- or unsubstituted-four membered heterocyclic ring, wherein
the substituents are selected from the group consisting of alkyl, halogen, -
C(=0)OR , and -OR ;
Re is selected from the group consisting of hydrogen, substituted- or
unsubstituted-alkyl, and substituted or unsubstituted cycloalkyl;
R9 is selected from the group consisting of hydrogen, substituted- or unsubstitutedalkyl,
substituted- or unsubstituted- alkenyl, substituted- or unsubstitutedalkynyl,
substituted- or unsubstituted- cycloalkyl and substitutedor
unsubstituted- cycloalkenyl;
n is an integer selected from the group consisting of 0, 1 and 2 ;
p is an integer selected from the group consisting of 0 and 1.
when the alkyl group and alkenyl group is substituted, the alkyl group and alkenyl
group is substituted with 1 to 4 substituents independently selected from the
group consisting of oxo, halogen, nitro, cyano, perhaloalkyl, cycloalkyl, aryl,
heteroaryl, heterocyclyl, -OR 10b , -SO 2R 10a , -C(=O)OR 10a , -OC(=O)R 0 , -C (=0)N(H)R ° ,
-ORio a -N(H)Rio, -N(alkyl)Rio
-N(H)C(=0)N(alkyl)R , -NH-S0 2-alkyl and -NH-S0 2-cycloalkyl;
when the cycloalkyl group and cycloalkenyl group is a substituted, the cycloalkyl
group and cycloalkenyl group is substituted with 1 to 3 substituents independently
selected from the group consisting of oxo, halogen, nitro, cyano, alkyl, alkenyl,
perhaloalkyl, aryl, heteroaryl, heterocyclyl, -OR 10b , -SO 2R 10a , -C(=O)R 10a , -
C(=O)OR 0a , -OC(=O)R 0a , -C (=0)N(H)R , -C (=0)N(alkyl)R , -N(H)C(=O)R 0a , -
N(H)Rio, -N(alkyl)Rio, -NH-S0 2-alkyl
and -NH-S0 2-cycloalkyl;
when the aryl group is a substituted, the aryl group is substituted with 1 to 3
substituents independently selected from the group consisting of halogen, nitro,
cyano, hydroxy, alkyl, alkenyl, perhaloalkyl, cycloalkyl, cycloalkenyl, heterocycle, -
O-alkyl, -O-perhaloalkyl, -N(alkyl)alkyl, -N(H)alkyl, -NH2, -S0 2-alkyl, -S0 2-
perhaloalkyl, -N(alkyl)C(=0)alkyl, -N(H)C(=0)alkyl, -C(=0)N(alkyl)alkyl,
C(=0)N(H)alkyl, -C(=0)NH2, -S0 2N(alkyl)alkyl, -S0 2N(H)alkyl, -S0 2NH2, -NH-S0 2-
alkyl and -NH-S0 2-cycloalkyl;
when the heteroaryl group is a substituted, the heteroaryl group is substituted
with 1 to 3 substituents independently selected from the group consisting of
halogen, nitro, cyano, hydroxy, alkyl, alkenyl, perhaloalkyl, cycloalkyl,
cycloalkenyl, heterocycle, -O-alkyl, O-perhaloalkyl, -N(alkyl)alkyl, -N(H)alkyl, -NH2,
-S0 2-alkyl, -S0 2-perhaloalkyl, -N(alkyl)C(=0)alkyl, -N(H)C(=0)alkyl,
C(=0)N(alkyl)alkyl, -C(=0)N(H)alkyl, -C(=0)NH2, -S0 2N(alkyl)alkyl, -S0 2N(H)alkyl, -
S0 2NH2, -NH-S0 2-alkyl and -NH-S0 2-cycloalkyl;
when the heterocyclyl group is a substituted, the heterocyclyl group is substituted
with 1 to 3 substituents, when the heterocyclic group is substituted on a ring
carbon of the 'heterocycle', the substituents are independently selected from the
group consisting of halogen, nitro, cyano, oxo, alkyl, alkenyl, perhaloalkyl,
cycloalkyl, cycloalkenyl, aryl, heteroaryl, heterocyclyl, -OR 10b , -C(=O)OR 10a , -
OC(=O)R 0a , -C (=0)N(H)R , -C(=0)N(alkyl)R ° , -N(H)C(=O)R 0 , -N(H)R °, -N(alkyl)R ,
-N(H)C (=0)N(H)R , -N(H)C(=O)N(alkyl)R ; the substituents on ring nitrogen of the
'heterocycle'; substituents are independently selected from the group consisting of
alkyl, alkenyl, cycloalkyl, cycloalkenyl, aryl, heteroaryl, -SO2R 10a , -C(=O)R 10a ,
C(=O)OR 0a -C (=0)N(H)R , -C (=0)N(alkyl)R °, -NH-S0 2-alkyl and -NH-S0 2-
cycloalkyl;
R 10 is selected from the group consisting of hydrogen, alkyl, alkenyl, cycloalkyl,
cycloalkenyl, aryl, heteroaryl, and heterocyclyl;
R i oa
S selected from the group consisting of alkyl, alkenyl, perhaloalkyl, cycloalkyl,
cycloalkenyl, aryl, heteroaryl, and heterocyclyl; and
R i ob s selected from the group consisting of hydrogen, alkyl, alkenyl, perhaloalkyl,
cycloalkyl, cycloalkenyl, aryl, heteroaryl, and heterocyclyl.
3. A compound of the general formula lb, its tautomeric forms, its
pharmaceutically acceptable salts, their combinations with suitable medicament
and pharmaceutical compositions containing them,
wherein,
R1 is selected from the group consisting of hydrogen, substituted- or
unsubstituted-alkyl, substituted- or unsubstituted- cycloalkyl, and substituted- or
unsubstituted- heterocyclyl;
R3 is selected from the group consisting of hydrogen, substituted- or
unsubstituted- alkyl, and substituted- or unsubstituted- cycloalkyl;
R4 is selected from the group consisting of hydrogen, halogen, substituted- or
unsubstituted-alkyl, and substituted- or unsubstituted- cycloalkyl;
Ra and Rb are selected from the group consisting of hydrogen, halogen and
haloalkyl;
R7 is selected from the group consisting of substituted- or unsubstitutedcycloalkyl,
and substituted- or unsubstituted- cycloalkenyl;
when the alkyl group and alkenyl group is substituted, the alkyl group and alkenyl
group is substituted with 1 to 4 substituents independently selected from the
group consisting of oxo, halogen, nitro, cyano, perhaloalkyl, cycloalkyl, aryl,
heteroaryl, heterocyclyl, -OR 10b , -SO 2R10a , -C(=O)OR 10a , -OC(=O)R 10a, -C(=0)N(H)R 10 ,
-ORioa, -N(H)Rio, -N(alkyl)R
-N(H)C(=0)N(alkyl)R10 , -NH-SCb-alkyl and -NH-S02-cycloalkyl;
when the cycloalkyl group and cycloalkenyl group is a substituted, the cycloalkyl
group and cycloalkenyl group is substituted with 1 to 3 substituents independently
selected from the group consisting of oxo, halogen, nitro, cyano, alkyl, alkenyl,
perhaloalkyl, aryl, heteroaryl, heterocyclyl, -OR 10b , -SO 2R 10a , -C(=O)R 10a , -
N(H)Rio, -N(alkyl)R , -NH-S0 2-alkyl
and -NH-S0 2-cycloalkyl;
when the aryl group is a substituted, the aryl group is substituted with 1 to 3
substituents independently selected from the group consisting of halogen, nitro,
cyano, hydroxy, alkyl, alkenyl, perhaloalkyl, cycloalkyl, cycloalkenyl, heterocycle, -
O-alkyl, -O-perhaloalkyl, -N(alkyl)alkyl, -N(H)alkyl, -NH2, -S0 2-alkyl, -S0 2-
perhaloalkyl, -N(alkyl)C(=0)alkyl, -N(H)C(=0)alkyl, -C(=0)N(alkyl)alkyl,
C(=0)N(H)alkyl, -C(=0)NH2, -S0 2N(alkyl)alkyl, -S0 2N(H)alkyl, -S0 2NH2, -NH-S0 2-
alkyl and -NH-S0 2-cycloalkyl;
when the heteroaryl group is a substituted, the heteroaryl group is substituted
with 1 to 3 substituents independently selected from the group consisting of
halogen, nitro, cyano, hydroxy, alkyl, alkenyl, perhaloalkyl, cycloalkyl,
cycloalkenyl, heterocycle, -O -alkyl, O -perhaloalkyl, -N(alkyl)alkyl, -N(H)alkyl, -NH2,
-S0 2-alkyl, -S0 2-perhaloalkyl, -N(alkyl)C(=0)alkyl, -N(H)C(=0)alkyl,
C(=0)N(alkyl)alkyl, -C(=0)N(H)alkyl, -C(=0)NH2, -S0 2N(alkyl)alkyl, -S0 2N(H)alkyl, -
S0 2NH2, -NH-S0 2-alkyl and -NH-S0 2-cycloalkyl;
when the heterocyclyl group is a substituted, the heterocyclyl group is substituted
with 1 to 3 substituents, when the heterocyclic group is substituted on a ring
carbon of the 'heterocycle', the substituents are independently selected from the
group consisting of halogen, nitro, cyano, oxo, alkyl, alkenyl, perhaloalkyl,
cycloalkyl, cycloalkenyl, aryl, heteroaryl, heterocyclyl, -OR 10b , -C(=O)OR 10a , -
-N(H)Rio, -N(alkyl)R ,
the substituents on ring nitrogen of the
'heterocycle'; substituents are independently selected from the group consisting of
alkyl, alkenyl, cycloalkyl, cycloalkenyl, aryl, heteroaryl, -SO 2R 10a , -C(=O)R 10a ,
-NH-S0 2-alkyl and -NH-SO 2-
cycloalkyl;
R10 is selected from the group consisting of hydrogen, alkyl, alkenyl, cycloalkyl,
cycloalkenyl, aryl, heteroaryl, and heterocyclyl;
R10a is selected from the group consisting of alkyl, alkenyl, perhaloalkyl, cycloalkyl,
cycloalkenyl, aryl, heteroaryl, and heterocyclyl; and
Riob
S selected from the group consisting of hydrogen, alkyl, alkenyl, perhaloalkyl,
cycloalkyl, cycloalkenyl, aryl, heteroaryl, and heterocyclyl.
4. A compound of the general formula Ic, its tautomeric forms, its
pharmaceutically acceptable salts, their combinations with suitable medicament
and pharmaceutical compositions containing them,
wherein,
R1 is selected from the group consisting of hydrogen, substituted- or
unsubstituted-alkyl, substituted- or unsubstituted- cycloalkyl, and substituted- or
unsubstituted- heterocyclyl;
R3 is selected from the group consisting of hydrogen, substituted- or
unsubstituted- alkyl, and substituted- or unsubstituted- cycloalkyl;
R4 is selected from the group consisting of hydrogen, halogen, substituted- or
unsubstituted-alkyl, and substituted- or unsubstituted- cycloalkyl;
Ra and Rb are selected from the group consisting of hydrogen, halogen and
haloalkyl;
R8 is selected from the group consisting of substituted- or unsubstitutedalkyl,
substituted- or unsubstituted- alkenyl, substituted- or unsubstitutedalkynyl,
substituted- or unsubstituted- cycloalkyl, and substituted- or
unsubstituted- cycloalkenyl;
when the alkyl group and alkenyl group is substituted, the alkyl group and alkenyl
group is substituted with 1 to 4 substituents independently selected from the
group consisting of oxo, halogen, nitro, cyano, perhaloalkyl, cycloalkyl, aryl,
heteroaryl, heterocyclyl, -OR 10b , -SO 2R10a , -C(=O)OR 0 , -OC(=O)R 0 , -C(=0)N(H)R °,
-OR 0a , -C(=0)N(alkyl)R , -N(H)C(=O)R 0a , -N(H)R , -N(alkyl)R -N(H)C (=0)N(H)R ,
-N(H)C(=0)N(alkyl)R , -NH-S02-alkyl and -NH-S02-cycloalkyl;
when the cycloalkyl group and cycloalkenyl group is a substituted, the cycloalkyl
group and cycloalkenyl group is substituted with 1 to 3 substituents independently
selected from the group consisting of oxo, halogen, nitro, cyano, alkyl, alkenyl,
perhaloalkyl, aryl, heteroaryl, heterocyclyl, -OR 10b , -SO 2R10a, -C(=O)R 10a , -
C(=O)OR 0a , -OC(=O)R 0a , -C(=0)N(H)R , -C(=0)N(alkyl)R , -N(H)C(=O)R 0a, -
N(H)Rio, -N(alkyl)R , -NH-S02-alkyl
and -NH-S02-cycloalkyl;
when the aryl group is a substituted, the aryl group is substituted with 1 to 3
substituents independently selected from the group consisting of halogen, nitro,
cyano, hydroxy, alkyl, alkenyl, perhaloalkyl, cycloalkyl, cycloalkenyl, heterocycle, -
O-alkyl, -O-perhaloalkyl, -N(alkyl)alkyl, -N(H)alkyl, -NH2, -S0 2-alkyl, -S0 2-
perhaloalkyl, -N(alkyl)C(=0)alkyl, -N(H)C(=0)alkyl, -C(=0)N(alkyl)alkyl,
C(=0)N(H)alkyl, -C(=0)NH2, -S0 2N(alkyl)alkyl, -S0 2N(H)alkyl, -S0 2NH2, -NH-S0 2-
alkyl and -NH-S0 2-cycloalkyl;
when the heteroaryl group is a substituted, the heteroaryl group is substituted
with 1 to 3 substituents independently selected from the group consisting of
halogen, nitro, cyano, hydroxy, alkyl, alkenyl, perhaloalkyl, cycloalkyl,
cycloalkenyl, heterocycle, -O-alkyl, O-perhaloalkyl, -N(alkyl)alkyl, -N(H)alkyl, -NH2,
-S0 2-alkyl, -S0 2-perhaloalkyl, -N(alkyl)C(=0)alkyl, -N(H)C(=0)alkyl,
C(=0)N(alkyl)alkyl, -C(=0)N(H)alkyl, -C(=0)NH2, -S0 2N(alkyl)alkyl, -S0 2N(H)alkyl, -
S0 2NH2, -NH-S0 2-alkyl and -NH-S0 2-cycloalkyl;
when the heterocyclyl group is a substituted, the heterocyclyl group is substituted
with 1 to 3 substituents, when the heterocyclic group is substituted on a ring
carbon of the 'heterocycle', the substituents are independently selected from the
group consisting of halogen, nitro, cyano, oxo, alkyl, alkenyl, perhaloalkyl,
cycloalkyl, cycloalkenyl, aryl, heteroaryl, heterocyclyl, -OR10b , -C(=O)OR10a , -
-N(H)Rio, -N(alkyl)Rio,
the substituents on ring nitrogen of the
'heterocycle'; substituents are independently selected from the group consisting of
alkyl, alkenyl, cycloalkyl, cycloalkenyl, aryl, heteroaryl, -SO2R10a , -C(=O)R10a,
C(=O)OR 0a -C(=0)N(H)R , -C(=0)N(alkyl)R , -NH-S0 2-alkyl and -NH-S0 2-
cycloalkyl;
R10 is selected from the group consisting of hydrogen, alkyl, alkenyl, cycloalkyl,
cycloalkenyl, aryl, heteroaryl, and heterocyclyl;
Rioa S selected from the group consisting of alkyl, alkenyl, perhaloalkyl, cycloalkyl,
cycloalkenyl, aryl, heteroaryl, and heterocyclyl; and
Riob s selected from the group consisting of hydrogen, alkyl, alkenyl, perhaloalkyl,
cycloalkyl, cycloalkenyl, aryl, heteroaryl, and heterocyclyl.
5. The compound of any one of claims 1-4, wherein R1 is selected from the
group consisting of hydrogen, alkyl, and cycloalkyl.
6. The compound of any one of claims 1-5, wherein R1 is selected from the
group consisting of hydrogen, methyl and cyclopropyl.
7. The compound of any one of claims 1-6, wherein R3 is methyl.
8. The compound of any one of claims 1-7, wherein R4 is selected as methyl.
9. The compound of any one of claims 1-8, wherein Ra and Rb are halogen.
10. The compound of any one of claims 1-9, wherein Ra and Rb are
independently fluorine and iodine.
11. The compound of any one of claims 1-10, wherein R6 is selected from the
group consisting of direct bond, -[C(R )Rd]nNR9 , -[C(R )Rd]nO-, and -
NHC(=0)[C(R )Rd]P .
12. The compound of any one of claims 1-1 1, wherein R6 is selected from the
group selected from direct bond, -NH-, -0-, -CH20-, and -NHC(=0)-.
13. The compound of any one of claims 1-12, wherein E is substitutued- or
unsubstituted- four membered heterocyclic ring.
14. The compound of any one of claims 1-13, wherein E is selected from the
group consisting of 3-oxetane, 1-azetidine, l-azetidine-2-one and 3-azetidine
substituted- or unsubstituted- with methyl, fluoro, -C(=0)ORe and -ORe; wherein Re
is hydrogen, tert-butyl, and -CH2C(=0)NH2.
15. The compound of any one of claims 1-14, wherein R7 is cyclopropyl.
16. The compound of any one of claims 1-15, wherein R8 is cyclopropyl.
17. The compound of any one of claims 1-16, wherein R1 is selected from the
group consisting of hydrogen, alkyl, cycloalkyl; R3 is alkyl; R4 is alkyl; Ra and Rb
are halogen; R6 is selected from the group consisting of direct bond, -[C(R )Rd]nNR9 ,
-[C(R )Rd]nO- and -NHC(=0)[C(R )Rd]P ; E is substitutued- or unsubstituted- four
membered heterocyclic ring; R7 is substituted- or unsubstituted- cycloalkyl; R8 is
substituted- or unsubstituted- cycloalkyl.
18. The compound of any one of claims 1-17, wherein R1 is selected from the
group consisting of hydrogen, methyl, cyclopropyl; R3 is methyl; R4 is methyl; Ra
and Rb are fluoro and iodo; R6 is direct bond, -NH , -0-, -CH20 - and -NHC(=0 ) ; E
is 3-oxetane, 1-azetidine, l-azetidine-2-one and 3-azetidine substituted- or
unsubstituted- with methyl, fluoro, tert-butoxy carbonyl, -OH and -
R7 is cyclopropyl and R8 is cyclopropyl.
19. The compound of any one of claims 1-18, wherein the compound is selected
from the group consisting of -
l-(3-(cyclopropylsulfonyl)phenyl)-5-((2-fluoro-4-iodophenyl)amino)-6,8-dimethyl
pyrido [4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 1)
3-cyclopropyl- l-(2-fluoro-4-iodophenyl)-5-((3-(3-hydroxyazetidin- 1-
yl)phenyl)amino) -6,8-dimethylpyrido[2,3-d]pyrimidine-2,4,7(lH,3H,8H)-trione
(Compound 2)
3-cyclopropyl- l-(3-(cyclopropylsulfonyl)phenyl)-5-((2-fluoro-4-
iodophenyl)amino)-6,8- dimethylpyrido [4 ,3-d ]pyrimidine- 2,4 ,7(1H,3H,6H)-trione
(Compound 3)
3-cyclopropyl-5-((2-fluoro-4-iodophenyl)amino)-6,8-dimethyl-l-(3-(2-
oxoazetidin-l-yl)phenyl)pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione
(Compound 4)
3-cyclopropyl-5-((2-fluoro-4-iodophenyl)amino)-6,8-dimethyl-l-(3-(oxetan-3-
ylamino) phenyl)pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 5)
3-cyclopropyl-5-((2-fluoro-4-iodophenyl)amino)-6,8-dimethyl-l-(3-(oxetan-3-
yloxy) phenyl)pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 7)
l-(3-(azetidin-l-yl)phenyl)-3-cyclopropyl-5-((2-iluoro-4-iodophenyl)amino)-6,8-
dimethyl pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 8)
3-cyclopropyl-5-((2-iluoro-4-iodophenyl)amino)-l-(3-(3-hydroxyoxetan-3-
yl)phenyl)-6,8-dimethylpyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione
(Compound 9)
N-(3-(3-cyclopropyl-5-((2-iluoro-4-iodophenyl)amino)-6,8-dimethyl-2,4,7-trioxo-
3,4,6,7-tetrahydropyrido[4,3-d]pyrimidin-l(2H)-yl)phenyl)-3-methyloxetane-3-
carboxamide (Compound 10)
l-(3-(cyclopropanecarbonyl)phenyl)-3-cyclopropyl-5-((2-iluoro-4-
iodophenyl)amino)-6,8-dimethylpyrido [4 ,3-d ]pyrimidine-2,4,7(1H,3H,6H)-trione
(Compound 11)
l-(3-(cyclopropylsulfonyl)phenyl)-5-((2-fluoro-4-iodophenyl)amino)-3,6,8-
trimethyl pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 12)
5-((2-fluoro-4-iodophenyl)amino)-3,6,8-trimethyl-l-(3-((oxetan-3-yloxy)methyl)
phenyl)pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 13)
5-((2-iluoro-4-iodophenyl)amino)-l-(3-(3-hydroxyoxetan-3-yl)phenyl)-3,6,8-
trimethylpyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 14)
5-((2-iluoro-4-iodophenyl)amino)-3,6,8-trimethyl-l-(3-(oxetan-3-yloxy)phenyl)
pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 15)
l-(3-(azetidin-l-yl)phenyl)-5-((2-iluoro-4-iodophenyl)amino)-3,6,8-
trimethylpyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 16)
5-((2-iluoro-4-iodophenyl)amino)-3,6,8-trimethyl-l-(3-(oxetan-3-
ylamino)phenyl) pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound
17)
N-(3-(5-((2-fluoro-4-iodophenyl)amino)-3,6,8-trimethyl-2,4,7-trioxo-3,4,6
tetrahydro pyrido[4,3-d]pyrimidin- 1(2H)-yl)phenyl)-3-methyloxetane-3-
carboxamide (Compound 18)
2-((l-(3-(5-((2-fluoro-4-iodophenyl)amino)-3,6,8-trimethyl-2,4,7-trioxo-3,4,6,7-
tetra hydropyrido[4,3-d]pyrimidin- 1(2H)-yl)phenyl)azetidin-3-yl)oxy)acetamide
(Compound 19)
5-((2-iluoro-4-iodophenyl)amino)-l-(3-(3-iluorooxetan-3-yl)phenyl)-3,6,8-
trimethyl pyrido[4,3-d]pyrimidine-2,4,7(lH,3H,6H)-trione (Compound 20)
N-(3-(3-cyclopropyl-5-((2-iluoro-4-iodophenyl)amino)-6,8-dimethyl-2,4,7-trioxo-
3,4,6,7-tetrahydropyrido[4,3-d]pyrimidin- 1(2H)-yl)phenyl)azetidine-3-
carboxamide (Compound 21)
20. A pharmaceutical composition comprising a compound or apharmaceutically
acceptable salt of any one of claims 1-19 and one or more pharmaceutically
acceptable carriers, diluents, or excipients.
21. A method for inhibiting MEK enzymes comprising contacting said MEK
enzyme with a composition comprising a compound of formula I, la, lb, Ic their
tautomeric forms, their pharmaceutically acceptable salts as claimed in any one of
claims 1-19, in an amount sufficient to inhibit said enzyme, wherein said enzyme
is inhibited.
22. The method of claim 21, wherein said MEK enzyme is MEK kinase.
23. The method of claim 2 1 wherein said contacting occurs within a cell.
24. A method of treatment of a MEK mediated disorder in an individual suffering
from said disorder, comprising administering to said individual an effective amount
of a composition comprising a compound of formula I, la, lb, Ic their tautomeric
forms, or their pharmaceutically acceptable salts as claimed in any one of claims
1-19.
25. The method of claim 24, further comprising administering an additional
therapy.
26. The method of claim 25, wherein said additional therapy is radiation
therapy, chemotherapy or a combination of both.
27. The method of claim 24, further comprising administering at least one
additional therapeutic agent.
28. The method of claim 24, wherein said MEK mediated disorder is selected
from the group consisting of inflammatory diseases, infections, autoimmune
disorders. Stroke, ischemia, cardiac disorder, neurological disorders, fibrogenetic
disorders, proliferative disorders, hyperproliferative disorders, tumors, leukemias,
neoplasms, cancers, carcinomas, metabolic diseases and malignant diseases.
29. The method of claim 24, wherein said MEK mediated disorder is a
hyperproliferative disease.
30. The method of claim 24, wherein said MEK mediated disorder is cancer,
tumors, leukemias, neoplasms, or carcinomas.
31. The method of claim 24, wherein said MEK mediated disorder is an
inflammatory disease.
32. The method of claim 24, wherein said individual is a mammal.
33. A method for the treatment or prophylaxis of a proliferative disease in an
individual in need thereof comprising administering to said individual an effective
amount of a composition comprising a compound of formula I, la, lb, Ic their
tautomeric forms, their pharmaceutically acceptable salts as claimed in claim 1-19.
34. The method of claim 33, wherein said proliferative disease is cancer,
psoriasis, restenosis, autoimmune disease, or atherosclerosis.
35. A method for the treatment or prophylaxis of an inflammatory disease in an
individual in need thereof comprising administering to said individual an effective
amount of a composition comprising a compound of formula I, la, lb, Ic their
tautomeric forms, or their pharmaceutically acceptable salts as claimed in claim 1-
19.
36. The method of claim 35, wherein said inflammatory disease is rheumatoid
arthritis or multiple sclerosis.
37. A method for degrading, inhibiting the growth of or killing cancer cells
comprising contacting the cells with an effective amount of a composition to
degrade, inhibit the growth of or kill cancer cells, wherein the composition
comprises a compound of formula I, la, lb, Ic their tautomeric forms, or their
pharmaceutically acceptable salts as claimed in claims 1-19.
38. A method of inhibiting tumor size increase, reducing the size of a tumor,
reducing tumor proliferation or preventing tumor proliferation in an individual
thereof comprising administering to said individual an effective amount of a
composition to inhibit tumor size increase, reduce the size of a tumor, reduce
tumor proliferation or prevent tumor proliferation, wherein the composition
comprises a compound of formula I, la, lb, Ic their tautomeric forms, or their
pharmaceutically acceptable salts, as claimed in claims 1-19.