DESC:FIELD OF INVENTION:
The present invention generally relates to production of hydrogen for use in hydrogen therapy. More particularly, the present invention relates to a device for generating oxygen and hydrogen with controlled quantity ratio in presence of electrocatalyst.

BACKGROUND AND PRIOR ART:
Hydrogen plays an important role across all industrial sectors as it is essential for various chemical processes and uses such as oil refining, production of ammonia through the Haber process, production of methanol through reduction of carbon monoxide, etc.

Hydrogen gas was first artificially produced in the early 16th century by reaction of acids on metals. Since then, there have been various methods of producing hydrogen which include steam reforming of natural gas and other light hydrocarbons from fossil fuels, partial oxidation of heavier hydrocarbons, coal gasification, biomass gasification, methane pyrolysis, and electrolysis of water. The four main sources for commercial production of hydrogen are natural gas, oil, coal, and electrolysis. Electrolysis consists of using electricity to split water into hydrogen and oxygen. The method of electrolysis of water for producing hydrogen is 70–80% efficient with a 20–30% conversion loss.

Further, the use of hydrogen as medical gas in hydrogen therapy for effectively reducing melanoma tumors in mice was discovered and reported by Dole and colleagues from Baylor University and Texas A&M in the year 1975. As such, hydrogen gas has been gaining significant attention from academic researchers, medical doctors, and physicians around the world for its therapeutic potential. It was further proved that administration of hydrogen gas via inhalation or ingestion of an aqueous-solution containing dissolved hydrogen, could also exert therapeutic biological effects. Thus, hydrogen emerges to be used in various medical and clinical applications due to its immediate medical applications to help with many of the current health crises.

Hydrogen gas (H2) has thus emerged as gas signaling molecule which possess multiple bioactivities, including anti-inflammation, anti-reactive oxygen species, anti-cancer, hormone-regulating, and apoptosis-resistance properties. The use of hydrogen as a therapeutic agent can also reduce the destructive cytokine storm and lung injury caused by SARS-CoV-2 viruses, seen during COVID19 pandemic, in the early stage, stimulating ropy sputum drainage, and ultimately reducing the incidence of severe disease. Hence, molecular hydrogen treatment is emerging as a new therapy.

However, the challenge of chemically generated hydrogen and oxygen is that, the hydrogen and oxygen produced by electrolysis of water using NaOH is very less. Thus, there is a need to improve hydrogen and oxygen production effectively to fill the huge demand for medical hydrogen and oxygen.

Thus, there is a need for fabricating a portable device for producing H2 and O2 gas, and to provide affordable healthcare.

The present invention thus discloses a device to produce hydrogen and oxygen in a cost-effective manner, and to meet the huge demand for patients who need hydrogen therapy worldwide. The said device according to present invention can cater to the need of a broad spectrum of patients suffering from inflammation, to even those undergoing adjuvant therapies, to those suffering from Parkinsonism, and any envisaged disease thereof, where hydrogen therapy is or to be proven to be of use.

SUMMARY OF THE INVENTION:
It is an object of the present invention to significantly and efficiently increase the production of hydrogen and oxygen.

It is another object of the present invention to produce hydrogen and oxygen in a cost effective and affordable manner.

It is another object of the present invention to tremendously enhance the generation of hydrogen and oxygen.

It is another object of the present invention to generate hydrogen and oxygen in a controlled quantity ratio by electrolyzing water.

The present invention thus discloses a simple, portable, cost-effective device for electrolyzing water using aqueous salts as a catalyst, and generating oxygen and hydrogen with controlled quantity ratio.

Accordingly, the present invention discloses a hydrogen aeration machine automat (HAMA) device for generating hydrogen and oxygen in controlled quantity ratio comprising, a reaction inducing tube containing an electrolyte solution, with one or more opening each having a removably covered closure means; plurality of electrodes fastened at each closure means of said reaction inducing tube through a class stick and immersed in the electrolyte solution; a gas pump and a hydrogen exhaust outlet, secured within one of said closure means having negative electrodes to increase hydrogen and oxygen output; wherein one of the removably covered closure means of said reaction inducing tube is coupled with negative electrodes, gas pump and a hydrogen exhaust outlet; and the other removably covered closure means of said reaction inducing tube is coupled with positive electrodes and an oxygen exhaust outlet.

The said reaction inducing tube contains water and catalyst solution and is preferably a U-shaped hollow cylindrical pipe with removable closure means for covering the openings.

The said plurality of electrodes is fastened at each closure means of said reaction inducing tube through a class stick and immersed in the water and catalyst solution (electrolytic solution).

Further, said reaction inducing tube comprises at least six number of said electrodes secured on each removable closure means.

The said electrolyte solution according to the present invention, for electrolyzing water and generating oxygen and hydrogen comprises a mixture of cobalt salts, urea and base as catalyst, NaOH solution, water and persulfate.

In accordance with one of the embodiment, the persulfate is in the range of 1-10 mg.

Further, the said gas pump (4) and plurality of electrodes (2) at both closure means (8), are connected to a battery for power supply.

Further, the said battery, electrodes and gas pump are interconnected using plurality of connecting wires.

The said housing unit encloses said reaction inducing tube along with the battery, and comprises the hydrogen exhaust outlet, the oxygen exhaust outlet and an LED button switch.

The method of generating hydrogen and oxygen in controlled quantity ratio using said hydrogen aeration machine automat (HAMA) device comprises the steps of, filling the reaction inducing tube with an electrolyte solution; supplying power to the electrodes and actuating the gas pump for enhancing hydrogen generation; generating hydrogen from negative electrode and oxygen from positive electrode; discharging hydrogen gas and oxygen gas from the hydrogen exhaust outlet and the oxygen exhaust outlet respectively.

Further, the said gas pump, coupled with hydrogen generation from negative electrodes helps to produce hydrogen to about 68%, as compared to the conventional chemically generated hydrogen which produces less gas volume.

The present invention thus provides a Hydrogen Aeration Machine Automat (HAMA) device by combining a pneumatic system with a reaction inducing tube, for electrolyzing water using aqueous catalyst, and generating oxygen and hydrogen in controlled quantity ratio.

BRIEF DESCRIPTION OF DRAWINGS:
The figures below show an exemplary embodiment:
Figure 1 illustrates a schematic diagram of the Hydrogen Aeration Machine Automat (HAMA) device.
Figure 1.1 illustrates a photographic representation of said Hydrogen Aeration Machine Automat (HAMA) device.
Figure 2 illustrates a schematic graph representation of comparison of retention time for pure oxygen from oxygen cylinder and a sample from said Hydrogen Aeration Machine Automat (HAMA) device.
Figure 3 illustrates a schematic graph representation of comparison of Mass Spectroscopy of pure oxygen from oxygen cylinder and the sample from said Hydrogen Aeration Machine Automat (HAMA) device.
Figure 4 illustrates a schematic graph representation of absorption of gas sample from said Hydrogen Aeration Machine Automat (HAMA) device at various time intervals.
Figure 5 illustrates a schematic graph representation of reduction in absorbance of methylene blue.
Figure 6 illustrates a schematic graph representation of concentration of methylene blue at different time intervals with reduction.
Figure 7 illustrates a schematic graph representation of change in concentration of methylene blue due to reduction.
Figure 8 illustrates a schematic graph representation of effect of said Hydrogen Aeration Machine Automat (HAMA) device in containing cancer demonstrated using HeLa cell lines.
List of reference numerals
Part ref Description
10 Hydrogen Aeration Machine Automat (HAMA) device
1 Reaction inducing tube
2 Screw electrode
3 Battery
4 Gas pump
5 Connecting cables
6 Hydrogen exhaust outlet
7 Oxygen exhaust outlet
8 Removable closure means
9 Housing unit

DETAILED DESCRIPTION OF THE INVENTION:
The present invention will now be described in detail with reference to optional and preferred embodiments so that various aspects of the invention will be more clearly understood, however, should not be construed to limit the scope of the invention. The invention is further described herein in detail with the help of figures appended at the end of the specification. The figures illustrate the preferred embodiment as well as other embodiments that define the scope of the present invention. However, it may be understood that the figures presented herein are intended to exemplify the scope of the invention only. The person skilled in art may note that by no means the figures limit the scope of the invention. Any variation in the drawings by any other person will be falling in the scope of the present invention.

Hydrogen gas (H2) has emerged as gas signaling molecule which possess multiple bioactivities, including anti-inflammation, anti-reactive oxygen species, anti-cancer, hormone-regulating, and apoptosis-resistance properties. Hence, there is an urgent need to improve hydrogen and oxygen production effectively to fill the huge demand for medical hydrogen and oxygen.

The present invention relates to a device for electrolyzing water using an electrocatalyst and generating oxygen and hydrogen with controlled quantity ratio.

Figure 1 and Figure 1.1 discloses a schematic view of the Hydrogen Aeration Machine Automat (HAMA) device (10) for electrolyzing water using an electrocatalyst and generating oxygen and hydrogen with controlled quantity ratio. The said reaction inducing tube (1) containing water and catalyst solution comprises one or more openings each having a removable closure means (8). The said plurality of electrodes (2) is fastened at each closure means (8) of said reaction inducing tube (1) through a class stick and immersed in the water and catalyst solution (electrolytic solution). Further, one of the removably covered closure means (8) of said reaction inducing tube (1) is coupled with negative electrodes, said gas pump (4) and a hydrogen exhaust outlet (6), and the other removably covered closure means (8) of said reaction inducing tube (1) is coupled with positive electrodes and an oxygen exhaust outlet (7). The said battery (3) is a rechargeable battery and is connected to said negative electrodes, positive electrodes and the gas pump (4).

Figure 2 discloses a schematic representation of comparison of retention time for pure oxygen from oxygen cylinder and a sample from said Hydrogen Aeration Machine Automat (HAMA) device. The retention time for pure oxygen taken from the oxygen cylinder is 1.55, and that of oxygen from the sample mixture is 1.54, thus the retention time of the sample mixture from Hydrogen Aeration Machine Automat (HAMA) device is almost the same as that of the pure oxygen from the oxygen cylinder.

Figure 3 illustrates a schematic representation of comparison of mass spectroscopy of pure oxygen from oxygen cylinder and the sample from said Hydrogen Aeration Machine Automat (HAMA) device. The peak at 28 m/Z value of MS of the sample is due to the nitrogen gas which is the major component of the ongoing reaction. The peak at 32 m/Z value is for oxygen and the 40 is that of Argon which is added to the Tedler bag for flushing out environmental nitrogen.

Figure 4 illustrates a schematic graph representation of absorption of gas sample from said HAMA device (1) at various time intervals of 0 (t0), 10 (t1), 20 (t2) and 30 (t3) minutes.

Figures 5-7 and table 1 illustrates the production of hydrogen by reducing methylene blue to leuco methylene blue.

Figure 5 illustrates a schematic graph representation of reduction in absorbance of methylene blue. Further, at wavelength 663 nm, the absorbance of the aqueous methylene blue solution is reduced with the progress of the hydrogen addition to the system.

Figure 6 illustrates a schematic graph representation of change in concentration of methylene blue at different time with reduction. The absorbance of 0.03 mM solution before the addition of hydrogen is 0.4, thus molar extinction coefficient will be 13333.3 cm-1/M.

Figure 7 illustrates a schematic graph representation of change in concentration of methylene blue (MB) due to reduction. Further, with increasing volume of the sample air supplied, the concentration of MB solution decreases, which is only possible if the sample air has hydrogen, which is added to MB to give leuco-MB.
7a) The concentration of MB solution= 0.03mM, 0 min of sample air purging
7b) The concentration of MB solution= 0.021mM, 10 min of sample air purging
7c) The concentration of MB solution= 0.019mM, 20 min of sample air purging
7d) The concentration of MB solution= 0.018mM, 30 min of sample air purging
The decreasing concentration with increasing purging of the gas from HAMA device clearly indicates the production of hydrogen that reduces MB to leuco-MB and thereby reducing the concentration of the MB, proving H2 production with the machine. The quantitative calculation is shown in Table 1. The below shown table describes the calculation of hydrogen production:

Figure 8 illustrates a schematic graph representing the effect of HAMA device (1) in containing cancer using HeLa cell lines. Hypoxia condition was induced to the media by purging the HeLa Cell line for 3 min with HAMA output gas and then allowing the cell to sustain in the media for a different amount of time shows the result that cell viability is decreasing significantly which can be implied as “the HeLa undergo Necrosis under the applied condition”. The media contains DMEM media+10% fetal bovine serum+1% anti-bacterial solution. The cell viability decreased from 97.05% to 57.14% within 100 minutes (almost 40%). The live cell numbers (per ml) decreased from 66 to 32 within 100 minutes and the dead cell numbers (per ml) increased from 2 to 24. Such observation indicates that the hypoxia induced in the media by HAMA output gas suppress the Cancer Hela cell significantly.

In a preferred embodiment according to the present invention, said hydrogen aeration machine automat (HAMA) device (10) for generating hydrogen and oxygen in controlled quantity ratio comprises, a reaction inducing tube (1) containing an electrolyte solution, with one or more opening each having a removably covered closure means (8); plurality of electrodes (2) fastened at each closure means (8) of said reaction inducing tube (1) through a class stick and immersed in the electrolyte solution; a gas pump (4) and a hydrogen exhaust outlet (6), secured within one of said closure means (8) having negative electrodes to increase hydrogen and oxygen output; wherein one of the removably covered closure means (8) of said reaction inducing tube (1) is coupled with negative electrodes, gas pump (4) and a hydrogen exhaust outlet (6); and the other removably covered closure means (8) of said reaction inducing tube (1) is coupled with positive electrodes and an oxygen exhaust outlet (7).

In a same preferred embodiment according to the present invention, said reaction inducing tube (1) is a U-shaped cylindrical pipe selected from a medically compatible plastic contraption.

In a same preferred embodiment according to the present invention, said electrodes (2) are selected from screw electrodes.

In a same preferred embodiment according to the present invention, a battery (3) is connected to said gas pump (4) and plurality of electrodes (2) at both closure means (8), for supplying power.

In a same preferred embodiment according to the present invention, said openings of the reaction inducing tube (1) comprises at least six number of said electrodes secured on the removable closure means (8).

In a same preferred embodiment according to the present invention said reaction induction tube (1) along with the battery (3), the hydrogen exhaust outlet (6) and the oxygen exhaust outlet (7) are enclosed in a housing unit (9) with a LED button switch.

In a same preferred embodiment according to the present invention said housing unit is a wooden rectangular box.

In a same preferred embodiment according to the present invention said battery (3), electrodes (2) and gas pump (4) are interconnected using plurality of connecting wires.

In a same preferred embodiment according to the present invention, the method of generating hydrogen and oxygen in controlled quantity ratio using a hydrogen aeration machine automat (HAMA) device (10) comprises the steps of, filling the reaction inducing tube (1) with an electrolyte solution; supplying power to the electrodes (2) and actuating a gas pump (4) for enhancing hydrogen generation; generating hydrogen from negative electrode and oxygen from positive electrode; discharging hydrogen gas and oxygen gas from the hydrogen exhaust outlet (6) and the oxygen exhaust outlet (7) respectively.

In a preferred embodiment according to the present invention, said electrolyte solution for electrolyzing water and generating oxygen and hydrogen, comprises a mixture of cobalt salts, urea and base as catalyst, NaOH solution, water and 1-10mg of persulfate.

In an exemplary embodiment according to the present invention, said reaction inducing pipe (1) comprises an aqueous salt solution containing cobalt acetate and urea as the catalyst.

In a further exemplary embodiment according to the present invention, said reaction inducing pipe (1) comprises a reaction solution containing water, sodium hydroxide and cobalt acetate as catalyst in fixed proportions.

In a preferred embodiment according to the present invention, said battery (3) is a 12V rechargeable battery capable to supply power for almost 4 hours.

In a further preferred embodiment according to the present invention, the gas pump (4) combination with the hydrogen exhaust outlet (6) boosts water splitting and enhances hydrogen generation and oxygen gets mixed with less volume as side product.

In a preferred embodiment according to the present invention, a hydrogen exhaust outlet (6) is connected from the top of the said housing unit (9) and the oxygen exhaust outlet (7) is linked from the back side of the said housing unit (9).

In the same preferred embodiment according to the present invention a LED switch button is fixed at the front of the said housing unit (9).

In the further preferred embodiment according to the present invention said HAMA device (10) weighs around 800 g and is easy to be carried around.

In a most preferred embodiment according to the present invention, the said HAMA device (10) comprises, a U-shape PVC pipe (1), twelve screw electrode of 3.5 inch long and 1/4-inch radius (2), a battery (IN 220V and Out 12V with 2A current) (3), a gas pump (4) and connecting cables (5) including connecting wires.

In an exemplary embodiment according to the present invention, said HAMA device (10) comprises, connecting cables (5) of 10-gauge wire red wire for positive indication and 10-gauge black wire for negative indication; 12 numbers of screw electrode of 3.5 inch long and ¼ inch radius; a transformer (IN 240V and OUT 24V with 2A current); a diode bridge of 6A and 500V; a gas pump; a u-shaped PVC pipe; a cyphonically connected water inlet; water; a mixture of cobalt salts, urea and base as catalyst, NaOH solution and 1-10mg of persulfate.

In a preferred embodiment according to the present invention, a subject can breathe-in the hydrogen-rich gas by switching on the power supply LED switch on the housing unit (9).

In an embodiment, the present invention relates to the use of HAMA device for hydrogen therapy.

In another embodiment, the present invention relates to the use of HAMA device for treating cancer.

In yet another embodiment, the present invention relates to a method for treating cancer comprising administering /inhaling the hydrogen gas evolved from said HAMA device.

Advantages:
• The device is rechargeable and can run for 4 hours without current supply.
• Use of pump system and catalyst results in higher production of hydrogen with controlled generation ratio.
• The output is a gas mix of mainly hydrogen (about 40%) and oxygen.
• The device is easy to recharge and the whole device is running on a cost and energy effective pattern.
• The device is simple, portable, cost effective, can be implemented at home and is scalable as well.

The foregoing descriptions are merely specific embodiments of the present invention, but are not intended to limit the protection scope of the present invention. Any variation or replacement readily figured out by a person skilled in the art within the technical scope disclosed in the present invention shall fall within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
,CLAIMS:1. A hydrogen aeration machine automat (HAMA) device (10) for generating hydrogen and oxygen in controlled quantity ratio comprising;
a reaction inducing tube (1) containing an electrolyte solution, with one or more openings each having a removably covered closure means (8);
plurality of electrodes (2) fastened at each closure means (8) of said reaction inducing tube (1) through a class stick and immersed in the electrolyte solution;
a gas pump (4) and a hydrogen exhaust outlet (6), secured within one of said closure means (8) having negative electrodes to increase hydrogen and oxygen output;
wherein one of the removably covered closure means (8) of said reaction inducing tube (1) is coupled with negative electrodes, gas pump (4) and a hydrogen exhaust outlet (6); and the other removably covered closure means (8) of said reaction inducing tube (1) is coupled with positive electrodes and an oxygen exhaust outlet (7).

2. The HAMA device (10) as claimed in claim 1, wherein said reaction inducing tube (1) is a U-shaped cylindrical pipe selected from a medically compatible plastic contraption.

3. The HAMA device (10) as claimed in claim 1, wherein said electrodes (2) are selected from screw electrodes.

4. The HAMA device (10) as claimed in claim 1, wherein a battery (3) is connected to said gas pump (4) and plurality of electrodes (2) at both closure means (8), for supplying power.

5. The said HAMA device (10) as claimed in claim 4, wherein said battery (3) is a rechargeable battery capable of supplying power for 4 hours.

6. The said HAMA device (10) as claimed in claim 1, wherein said openings of the reaction inducing tube (1) comprises at least six number of said electrodes secured on the removable closure means (8).

7. The said HAMA device (10) as claimed in claim 1, wherein said reaction induction tube (1) along with the battery (3), the hydrogen exhaust outlet (6) and the oxygen exhaust outlet (7) are enclosed in a housing unit (9) with a LED button switch.

8. The said HAMA device (10) as claimed in claim 7, wherein said housing unit is a wooden rectangular box.

9. The HAMA device (10) as claimed in claim 1, wherein said battery (3), electrodes (2) and gas pump (4) are interconnected using plurality of connecting wires.

10. A method of generating hydrogen and oxygen in controlled quantity ratio in a hydrogen aeration machine automat (HAMA) device (10) comprising,
filling the reaction inducing tube (1) with an electrolyte solution;
supplying power to the electrodes (2) and actuating a gas pump (4) for enhancing hydrogen generation;
generating hydrogen from negative electrode and oxygen from positive electrode;
discharging hydrogen gas and oxygen gas from the hydrogen exhaust outlet (6) and the oxygen exhaust outlet (7) respectively.

11. The method as claimed in claim 10, wherein said electrolyte solution comprises a mixture of cobalt salts, urea and base as catalyst, NaOH solution, water and persulfate.

12. The method as claimed in claim 11, wherein the cobalt salt is cobalt acetate.
13. An electrolyte solution for electrolyzing water for generating oxygen and hydrogen comprising a mixture of cobalt salts, urea and base as catalyst, NaOH solution, water and persulfate.

14. The electrolyte as claimed in Claim 13, wherein the persulfate is in range of 1-10 mg.

15. The electrolyte as claimed in Claim 13, wherein the cobalt salt is cobalt acetate.

16. Use of hydrogen aeration machine automat (HAMA) device (10) for hydrogen therapy.

17. Use of hydrogen aeration machine automat (HAMA) device (10) for cancer treatment.

18. A method for treating cancer comprising administering /inhaling the hydrogen gas evolved from said HAMA device.