Description:FIELD OF THE INVENTION

Our Invention is related to a Hyperbaric Pressure Chamber with Automatic Pressure Control System.

BACKGROUND OF THE INVENTION

We looked at how exposure to hyperbaric oxygen affected the skeletal muscles' ability to oxidize in mice of various ages.

For two weeks, we subjected 5-, 34-, 55-, and 88-week-old mice to 6 hours a day of 36% oxygen at 950 mmHg.

Under normobaric settings (21% oxygen at 760 mmHg), the activities of the mitochondrial marker enzyme succinate dehydrogenase (SDH) in the tibialis anterior muscle of hyperbaric mice were compared with those of age-matched animals.

Furthermore, quantitative histochemical analysis was used to assess the SDH activities of type IIA and type IIB fibers in the muscle.

In normobaric mice, the muscle's SDH activity decreased with age. The muscle's type IIA and type IIB fibers showed comparable outcomes. In hyperbaric mice, the decline in muscle SDH activity was decreased at 57 and 90 weeks.

In hyperbaric mice, the lower SDH activities of type IIA and type IIB fibers were seen at 90 weeks, 57 weeks, and 90 weeks, respectively.

We draw the conclusion that the hyperbaric oxygen used in this investigation lessens the aging-related decline in skeletal muscle oxidative capability.

OBJECTIVES OF THE INVENTION
1. The objective of the invention is to provide a “HYPERBARIC PRESSURE CHAMBER WITH AUTOMATIC PRESSURE CONTROL SYSTEM” is a Pressure chamber, pressure sensor (PSI), AVR controller, Wi-Fi module, Auto On/Off relay, Solvonoid valve, LCD display ,Online pressure monitoring application.
2. The other objective of the invention is to provide a subject the growing cancer cells to increased pressure, an inert gas (N2) was used in addition to the usual 5% CO2. The following setups were done to best study the effect on pressure-treated cancer and normal cells (experimentals) versus the corresponding non-pressure-treated ones (controls):
3. The other objective of the invention is to provide a Control cancer cells (1x105 cells/mL) were placed in 25 cm2 flasks kept in a 5% CO2 incubator and maintained at normal atmospheric pressure (14.5 psi) while experimental pressure-treated cancer cells (1x105 cells/mL) were placed in a pressure chamber connected to CO2+N2 cylinder and maintained at a pressure of 18 psi inside the incubator.
4. The other objective of the invention is to provide a Control normal cells (1x105 cells/mL) were placed in 25 cm2 flasks kept in a 5% CO2 incubator and maintained at normal atmospheric pressure (14.5 psi) while experimental pressure-treated normal cells were placed in a pressure chamber connected to CO2+N2 cylinder and maintained at a pressure of 18 psi inside the incubator.
SUMMARY OF THE INVENTION

The partial pressure of oxygen is enhanced and the concentration of dissolved oxygen in the plasma is increased when there is a rise in atmospheric pressure and high oxygen concentration.

Oxidative metabolism in cells and tissues is increased by a rise in air pressure and oxygen content, which also raises the activity of mitochondrial oxidative enzymes.

In addition, when air pressure and oxygen concentration rise, so does the concentration of carbon dioxide, which promotes the release of oxygen from hemoglobin and induces blood vessels to dilate.

For the animal trials, we created a hyperbaric chamber that included an oxygen tank with an oxygen concentrator and an air compressor that uses a computer-assisted method to automatically maintain the high air pressure and oxygen concentration.

We identified the ideal air pressure (950 mmHg) and oxygen content (36%), which are necessary to achieve the desired effects on the neuromuscular system's oxidative capacity.

BRIEF DESCRIPTION OF THE DIAGRAM
Figure 1(a): Schematic drawing of the experimental setup.
Figure 1(b): Designed Pressure Chamber.
DESCRIPTION OF THE INVENTION
Hyperbaric Pressure Chamber consists of a pressure sensor which is connected to a control system. Control system has a LCD display, keypad and an AVR controller with enabled data transmission system.

The device has the ability to transfer online pressure datasheet 24*7 to a connected mobile or computer system (Fig. 1a). Pressure chamber is connected through a flexible silicon pipe to a gas cylinder containing CO2+N2 mixture.

There is a solvonoid valve between cylinder and pressure chamber which is connected to the controller system to maintain the pressure.

If the pressure within the chamber falls below 18 psi (set pressure), then the solvonoid valve automatically opens to allow CO2+N2 gas mixture to pass through it and maintain the desired pressure inside the chamber.

After the desired pressure is attained, the solvonoid valve closes automatically till the pressure drops again (Fig. 1b).
, Claims:I/WE CLAIMS
1) Our Invention “HYPERBARIC PRESSURE CHAMBER WITH AUTOMATIC PRESSURE CONTROL SYSTEM” is a Pressure chamber, pressure sensor (PSI), AVR controller, Wi-Fi module, Auto On/Off relay, Solvonoid valve, LCD display ,Online pressure monitoring application. To subject the growing cancer cells to increased pressure, an inert gas (N2) was used in addition to the usual 5% CO2. The following setups were done to best study the effect on pressure-treated cancer and normal cells (experimentals) versus the corresponding non-pressure-treated ones (controls): Control cancer cells (1x105 cells/mL) were placed in 25 cm2 flasks kept in a 5% CO2 incubator and maintained at normal atmospheric pressure (14.5 psi) while experimental pressure-treated cancer cells (1x105 cells/mL) were placed in a pressure chamber connected to CO2+N2 cylinder and maintained at a pressure of 18 psi inside the incubator. Control normal cells (1x105 cells/mL) were placed in 25 cm2 flasks kept in a 5% CO2 incubator and maintained at normal atmospheric pressure (14.5 psi) while experimental pressure-treated normal cells were placed in a pressure chamber connected to CO2+N2 cylinder and maintained at a pressure of 18 psi inside the incubator.
2) According to claim1# the invention is to a “HYPERBARIC PRESSURE CHAMBER WITH AUTOMATIC PRESSURE CONTROL SYSTEM” is a Pressure chamber, pressure sensor (PSI), AVR controller, Wi-Fi module, Auto On/Off relay, Solvonoid valve, LCD display ,Online pressure monitoring application.
3) According to claim1,2# the invention is to a subject the growing cancer cells to increased pressure, an inert gas (N2) was used in addition to the usual 5% CO2. The following setups were done to best study the effect on pressure-treated cancer and normal cells (experimentals) versus the corresponding non-pressure-treated ones (controls):
4) According to claim1,2,3# the invention is to a Control cancer cells (1x105 cells/mL) were placed in 25 cm2 flasks kept in a 5% CO2 incubator and maintained at normal atmospheric pressure (14.5 psi) while experimental pressure-treated cancer cells (1x105 cells/mL) were placed in a pressure chamber connected to CO2+N2 cylinder and maintained at a pressure of 18 psi inside the incubator.
5) According to claim1,2,3# the invention is to a Control normal cells (1x105 cells/mL) were placed in 25 cm2 flasks kept in a 5% CO2 incubator and maintained at normal atmospheric pressure (14.5 psi) while experimental pressure-treated normal cells were placed in a pressure chamber connected to CO2+N2 cylinder and maintained at a pressure of 18 psi inside the incubator.