DESC:Uploaded ,CLAIMS:We claim:
1. A composition comprising one or more unique genomic regions of table 1 for detection of disease.
2. A method of identifying unique genomic regions of claim 1 from the genome is comprising of the steps:
a) processing the genome into sliding fragments
b) aligning each of these fragments (a) with respective genome databases to identify consensus sequences and use of these consensus sequences as unique kmer fragments
c) fusion of unique kmer fragments (b).
3. The composition of claim 1, wherein the said composition is used for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-Cov2) or SARS-Cov2 variants.
4. The method of claim 2, wherein the said method is used to design detection kits for infectious disease.
5. The method of claim 2, wherein the said method is used to design a highly specific real time PCR detection kit for infectious disease.
6. A method for multiplexing multiple unique genomic regions of claim 1.
7. A method for detection of SARS-Cov2 simultaneously in mass population through amplicon sequencing by using the unique genomic regions of claim 1.
8. A method for detection of disease simultaneously in mass population through amplicon sequencing by using the method of claim 2.
9. The method of claim 2, wherein the said method is further used for Next-generation sequencing (NGS) based technologies.
10. The method of claim 2, wherein the said method is used for detection for SARS-Cov2 or SARS-Cov2 variants.
11. A method for designing targeted NGS panel for detection of SARS-Cov2 and/or SARS-Cov2 variants from a mixed population of viruses.
12. A method for designing molecular probes wherein the said method is used in NGS for future mutations in evolutionarily unique regions in any virus or other infectious disease-causing organisms
13. A high-throughput method for detection of infectious disease using targeted sequencing of evolutionarily unique regions
14. A high-throughput method for detection of SARS-Cov2 or SARS-Cov2 variants using targeted sequencing of evolutionarily unique regions to be used in detection and/or genome surveillance