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Immobilization Of Enzyme For Removal Of Contaminants
Abstract: Process for immobilization of an enzyme, characterized by the steps comprising (a) activating said support with 1-ethyl-3 (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysulfosuccinimide, (b) covalent coupling of activated support with said enzyme at a pH ranging from about 4.5 to 6.5 and temperature of 4°C, (c) packing of column with enzyme coupled support, (d) passing culture supernatant containing capsular polysaccharide of interest through the column followed by a suitable contact time, and (e) diafiltration with molecular weight cut-off ranging from about 100 kDa to 300 kDa of eluate to remove degraded nucleic acid ,protein and polysaccharide.The covalently immobilized enzymes affords substantial reduction of enzyme as a residual contaminant in the supernatant. The said immobilized enzymes can be recycled for atleast 4 times.
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Notices, Deadlines & Correspondence
Notices, Deadlines & Correspondence
Patent Information
Application #
Filing Date
22 April 2009
Publication Number
45/2010
Publication Type
INA
Invention Field
BIOTECHNOLOGY
Status
Email
Parent Application
Patent Number
Legal Status
Grant Date
2016-12-09
Renewal Date
Applicants
Serum Institute of India Ltd.
212/2 Off Soli Poonawalla Road Hadapsar Pune 411 028 Maharashtra India.
Inventors
1. KAPRE Subhash V.
c/o Serum Institute of India Ltd. of 212/2 Off Soli Poonawalla Road Hadapsar Pune 411 028 Maharashtra India.
2. CHHIKARA Manoj Kumar
c/o Serum Institute of India Ltd. of 212/2 Off Soli Poonawalla Road Hadapsar Pune 411 028 Maharashtra India.
Specification
attached
Documents
Orders
| Section | Controller | Decision Date |
|---|---|---|
Application Documents
| # | Name | Date |
|---|---|---|
| 1 | 1074-MUM-2009-FORM 18(14-11-2011).pdf | 2011-11-14 |
| 1 | 1074-MUM-2009-FORM-27 [27-09-2024(online)].pdf | 2024-09-27 |
| 2 | 1074-MUM-2009-CORRESPONDENCE(14-11-2011).pdf | 2011-11-14 |
| 2 | 1074-MUM-2009-RELEVANT DOCUMENTS [27-09-2023(online)].pdf | 2023-09-27 |
| 3 | Other Document [05-01-2016(online)].pdf | 2016-01-05 |
| 3 | 1074-MUM-2009-RELEVANT DOCUMENTS [30-09-2022(online)].pdf | 2022-09-30 |
| 4 | Form 13 [05-01-2016(online)].pdf | 2016-01-05 |
| 4 | 1074-MUM-2009-RELEVANT DOCUMENTS [28-09-2021(online)].pdf | 2021-09-28 |
| 5 | 1074-MUM-2009-RELEVANT DOCUMENTS [19-03-2020(online)].pdf | 2020-03-19 |
| 5 | 1074-MUM-2009-GENERAL POWER OF ATTORNEY-(18-04-2016).pdf | 2016-04-18 |
| 6 | 1074-MUM-2009-RELEVANT DOCUMENTS [26-02-2019(online)].pdf | 2019-02-26 |
| 6 | 1074-MUM-2009-FORM 2(TITLE PAGE)-(18-04-2016).pdf | 2016-04-18 |
| 7 | 1074-MUM-2009-FORM 1-(18-04-2016).pdf | 2016-04-18 |
| 7 | 1074-MUM-2009-ABSTRACT(22-4-2010).pdf | 2018-08-10 |
| 8 | 1074-MUM-2009-CORRESPONDENCE-(18-04-2016).pdf | 2016-04-18 |
| 8 | 1074-MUM-2009-ABSTRACT(GRANTED)-(9-12-2016).pdf | 2018-08-10 |
| 9 | 1074-MUM-2009-CERTIFICATE OF INCORPORATION CONSEQUENT-(18-04-2016).pdf | 2016-04-18 |
| 9 | 1074-MUM-2009-CLAIMS(22-4-2010).pdf | 2018-08-10 |
| 10 | 1074-MUM-2009-CLAIMS(GRANTED)-(9-12-2016).pdf | 2018-08-10 |
| 10 | OTHERS [30-05-2016(online)].pdf | 2016-05-30 |
| 11 | 1074-MUM-2009-CORRESPONDENCE(17-6-2009).pdf | 2018-08-10 |
| 11 | Other Document [30-05-2016(online)].pdf | 2016-05-30 |
| 12 | 1074-MUM-2009-CORRESPONDENCE(22-4-2010).pdf | 2018-08-10 |
| 12 | Marked Copy [30-05-2016(online)].pdf | 2016-05-30 |
| 13 | 1074-MUM-2009-CORRESPONDENCE(IPO)-(9-12-2016).pdf | 2018-08-10 |
| 13 | Form 13 [30-05-2016(online)].pdf | 2016-05-30 |
| 14 | 1074-MUM-2009-CORRESPONDENCE(IPO)-(DECISION)-(9-12-2016).pdf | 2018-08-10 |
| 14 | Examination Report Reply Recieved [30-05-2016(online)].pdf | 2016-05-30 |
| 15 | 1074-MUM-2009-CORRESPONDENCE(IPO)-(FER)-(12-6-2015).pdf | 2018-08-10 |
| 15 | Description(Complete) [30-05-2016(online)].pdf_94.pdf | 2016-05-30 |
| 16 | 1074-MUM-2009-CORRESPONDENCE(IPO)-(HEARING NOTICE)-(26-9-2016).pdf | 2018-08-10 |
| 16 | Description(Complete) [30-05-2016(online)].pdf | 2016-05-30 |
| 17 | Claims [30-05-2016(online)].pdf | 2016-05-30 |
| 17 | 1074-MUM-2009-DESCRIPTION(COMPLETE)-(22-4-2010).pdf | 2018-08-10 |
| 18 | 1074-MUM-2009-DESCRIPTION(GRANTED)-(9-12-2016).pdf | 2018-08-10 |
| 18 | Abstract [30-05-2016(online)].pdf | 2016-05-30 |
| 19 | 1074-MUM-2009-DRAWING(GRANTED)-(9-12-2016).pdf | 2018-08-10 |
| 19 | HEARING ADJOURNMENT [10-10-2016(online)].pdf | 2016-10-10 |
| 20 | 1074-MUM-2009-CORRESPONDENCE(IPO)-(HEARING NOTICE)-(10-11-2016).pdf | 2016-11-10 |
| 20 | 1074-MUM-2009-FORM 1(17-6-2009).pdf | 2018-08-10 |
| 21 | 1074-mum-2009-form 2(22-4-2010).pdf | 2018-08-10 |
| 21 | Other Patent Document [05-12-2016(online)].pdf | 2016-12-05 |
| 22 | 1074-MUM-2009-FORM 2(GRANTED)-(9-12-2016).pdf | 2018-08-10 |
| 22 | Form 27 [07-02-2017(online)].pdf | 2017-02-07 |
| 23 | 1074-MUM-2009-FORM 2(TITLE PAGE)-(22-4-2010).pdf | 2018-08-10 |
| 23 | 1074-MUM-2009-RELEVANT DOCUMENTS [07-03-2018(online)].pdf | 2018-03-07 |
| 24 | 1074-MUM-2009-FORM 2(TITLE PAGE)-(GRANTED)-(9-12-2016).pdf | 2018-08-10 |
| 24 | others_20160530172409.pdf | 2018-08-10 |
| 25 | 1074-MUM-2009-FORM 5(22-4-2010).pdf | 2018-08-10 |
| 25 | Form-3.pdf | 2018-08-10 |
| 26 | 1074-MUM-2009-POWER OF AUTHORITY(17-6-2009).pdf | 2018-08-10 |
| 26 | Form-1.pdf | 2018-08-10 |
| 27 | 1074-MUM-2009_EXAMREPORT.pdf | 2018-08-10 |
| 27 | complete spec_20160530172442.pdf | 2018-08-10 |
| 28 | 15509-22 fer response_20160530172348.pdf | 2018-08-10 |
| 28 | clean claims_20160530172538.pdf | 2018-08-10 |
| 29 | abstract_20160530172518.pdf | 2018-08-10 |
| 30 | 15509-22 fer response_20160530172348.pdf | 2018-08-10 |
| 30 | clean claims_20160530172538.pdf | 2018-08-10 |
| 31 | 1074-MUM-2009_EXAMREPORT.pdf | 2018-08-10 |
| 31 | complete spec_20160530172442.pdf | 2018-08-10 |
| 32 | 1074-MUM-2009-POWER OF AUTHORITY(17-6-2009).pdf | 2018-08-10 |
| 32 | Form-1.pdf | 2018-08-10 |
| 33 | 1074-MUM-2009-FORM 5(22-4-2010).pdf | 2018-08-10 |
| 33 | Form-3.pdf | 2018-08-10 |
| 34 | 1074-MUM-2009-FORM 2(TITLE PAGE)-(GRANTED)-(9-12-2016).pdf | 2018-08-10 |
| 34 | others_20160530172409.pdf | 2018-08-10 |
| 35 | 1074-MUM-2009-RELEVANT DOCUMENTS [07-03-2018(online)].pdf | 2018-03-07 |
| 35 | 1074-MUM-2009-FORM 2(TITLE PAGE)-(22-4-2010).pdf | 2018-08-10 |
| 36 | 1074-MUM-2009-FORM 2(GRANTED)-(9-12-2016).pdf | 2018-08-10 |
| 36 | Form 27 [07-02-2017(online)].pdf | 2017-02-07 |
| 37 | 1074-mum-2009-form 2(22-4-2010).pdf | 2018-08-10 |
| 37 | Other Patent Document [05-12-2016(online)].pdf | 2016-12-05 |
| 38 | 1074-MUM-2009-CORRESPONDENCE(IPO)-(HEARING NOTICE)-(10-11-2016).pdf | 2016-11-10 |
| 38 | 1074-MUM-2009-FORM 1(17-6-2009).pdf | 2018-08-10 |
| 39 | 1074-MUM-2009-DRAWING(GRANTED)-(9-12-2016).pdf | 2018-08-10 |
| 39 | HEARING ADJOURNMENT [10-10-2016(online)].pdf | 2016-10-10 |
| 40 | 1074-MUM-2009-DESCRIPTION(GRANTED)-(9-12-2016).pdf | 2018-08-10 |
| 40 | Abstract [30-05-2016(online)].pdf | 2016-05-30 |
| 41 | 1074-MUM-2009-DESCRIPTION(COMPLETE)-(22-4-2010).pdf | 2018-08-10 |
| 41 | Claims [30-05-2016(online)].pdf | 2016-05-30 |
| 42 | 1074-MUM-2009-CORRESPONDENCE(IPO)-(HEARING NOTICE)-(26-9-2016).pdf | 2018-08-10 |
| 42 | Description(Complete) [30-05-2016(online)].pdf | 2016-05-30 |
| 43 | 1074-MUM-2009-CORRESPONDENCE(IPO)-(FER)-(12-6-2015).pdf | 2018-08-10 |
| 43 | Description(Complete) [30-05-2016(online)].pdf_94.pdf | 2016-05-30 |
| 44 | 1074-MUM-2009-CORRESPONDENCE(IPO)-(DECISION)-(9-12-2016).pdf | 2018-08-10 |
| 44 | Examination Report Reply Recieved [30-05-2016(online)].pdf | 2016-05-30 |
| 45 | 1074-MUM-2009-CORRESPONDENCE(IPO)-(9-12-2016).pdf | 2018-08-10 |
| 45 | Form 13 [30-05-2016(online)].pdf | 2016-05-30 |
| 46 | 1074-MUM-2009-CORRESPONDENCE(22-4-2010).pdf | 2018-08-10 |
| 46 | Marked Copy [30-05-2016(online)].pdf | 2016-05-30 |
| 47 | 1074-MUM-2009-CORRESPONDENCE(17-6-2009).pdf | 2018-08-10 |
| 47 | Other Document [30-05-2016(online)].pdf | 2016-05-30 |
| 48 | 1074-MUM-2009-CLAIMS(GRANTED)-(9-12-2016).pdf | 2018-08-10 |
| 48 | OTHERS [30-05-2016(online)].pdf | 2016-05-30 |
| 49 | 1074-MUM-2009-CLAIMS(22-4-2010).pdf | 2018-08-10 |
| 49 | 1074-MUM-2009-CERTIFICATE OF INCORPORATION CONSEQUENT-(18-04-2016).pdf | 2016-04-18 |
| 50 | 1074-MUM-2009-ABSTRACT(GRANTED)-(9-12-2016).pdf | 2018-08-10 |
| 50 | 1074-MUM-2009-CORRESPONDENCE-(18-04-2016).pdf | 2016-04-18 |
| 51 | 1074-MUM-2009-ABSTRACT(22-4-2010).pdf | 2018-08-10 |
| 51 | 1074-MUM-2009-FORM 1-(18-04-2016).pdf | 2016-04-18 |
| 52 | 1074-MUM-2009-FORM 2(TITLE PAGE)-(18-04-2016).pdf | 2016-04-18 |
| 52 | 1074-MUM-2009-RELEVANT DOCUMENTS [26-02-2019(online)].pdf | 2019-02-26 |
| 53 | 1074-MUM-2009-RELEVANT DOCUMENTS [19-03-2020(online)].pdf | 2020-03-19 |
| 53 | 1074-MUM-2009-GENERAL POWER OF ATTORNEY-(18-04-2016).pdf | 2016-04-18 |
| 54 | Form 13 [05-01-2016(online)].pdf | 2016-01-05 |
| 54 | 1074-MUM-2009-RELEVANT DOCUMENTS [28-09-2021(online)].pdf | 2021-09-28 |
| 55 | Other Document [05-01-2016(online)].pdf | 2016-01-05 |
| 55 | 1074-MUM-2009-RELEVANT DOCUMENTS [30-09-2022(online)].pdf | 2022-09-30 |
| 56 | 1074-MUM-2009-RELEVANT DOCUMENTS [27-09-2023(online)].pdf | 2023-09-27 |
| 56 | 1074-MUM-2009-CORRESPONDENCE(14-11-2011).pdf | 2011-11-14 |
| 57 | 1074-MUM-2009-FORM 18(14-11-2011).pdf | 2011-11-14 |
| 57 | 1074-MUM-2009-FORM-27 [27-09-2024(online)].pdf | 2024-09-27 |