530.00/1
Title: Improvements relating tn skin dressings
Field of the Invention
This invention relates to skin dressings for application to a part of a human or animal body
for treatment of skin (for therapeutic or cosmetic purposes), and relates particularly (but not
exclusively) to wound dressings for treatment of compromised skin, particularly skin lesions,
Le. any interruption in the surface of the skin, whether caused by injury or disease, including
skin ulcers, burns, cuts, punctures, lacerations, blunt traumas, acne lesions, boils etc. The
term "skin dressing" covers dressings such as patches, plasters, bandages and gauze etc. for
use in connection with transdermal delivery of agents. The term also includes material in
amorphous or liquid form. The term covers dressings for application to body surfaces
generally, including internal and external tissues, particularly the skin including the scalp.
The invention is based on the beneficial properties of nitric oxide (NO).
Background to the Invention
Nitric oxide has a multitude of effects in living tissues. The mechanism of these effects is
nearly always based on interaction of nitric oxide either with metal component (typically
iron) or with thiol groups of key enzymes and other proteins. Depending on the particular
enzyme, such interaction can lead to either activation or inhibition of the protein. An
example of an effect based on the activation of an enzyme is that of vasodilatation: nitric
oxide binds to the haem iron of the enzyme guanylate cyclase, which results in
conformational change exposing the catalytic site of the enzyme. This leads to catalytic
conversion of GTP to cGMP. This conversion initiates the whole cascade of reactions
leading to protein phosphorylation and muscle relaxation (vasodilatation). Other effects
based on activation of enzymes or growth factors by nitric oxide include stimulation of cell
division (proliferation) and cell maturation, stimulation of cell differentiation and formation
of cell receptors, neovascularisation, formation of fibroblasts in the wound and thereby
enhancement of collagen formation, etc.
Topical delivery of nitric oxide can be a very useful feature in various therapeutic or
cosmetic applications including wound healing, treatment of skin or nail infections, sexual
dysfunction etc.
Under normal conditions, nitric oxide (NO) is a short-lived, unstable gaseous substance. Its
instability is due to the unpaired electron of nitrogen. It is therefore beneficial to deliver
nitric oxide topically in the form of a nitric oxide donor which diffuses into the body site and
releases nitric oxide, either spontaneously or on activation.
Particularly useful nitric oxide donors are nitrosothiols. Nitrosothiols are donors of nitric
oxide which can be released by their spontaneous decomposition:

The rate of decomposition varies considerably depending on the side chain of the thiol. For
example, whilst nitrosocysteine can be almost totally decomposed within minutes under
normal conditions, it takes hours/days to achieve almost complete decomposition of
nitrosoglycerol. The decomposition can be accelerated markedly in the presence of Cu2+ and
Hg2*. Nitrosothiols are also able to donate nitric oxide directly onto another thiol group. This
process, which is called trans-nitrosation, is quite common in vivo:

Nitrosothiols can be produced by reaction between thiols and nitrite in acidic environment.
The reaction mechanism involves formation of nitrosonium cation (NO*) which, in turn,
reacts with a thiol to produce corresponding nitrosothiol:

S-nitrosothiols can thus be produced conveniently by mixing a thiol (e.g. thioglycerol) with a
source of nitrite (e.g. potassium nitrite) in acidic solution. The reaction proceeds at pH < 6,
the rate of the reaction increasing with the acidity of the solution:

WO2006709S193 discloses a skin dressing adapted, on activation, to release one or more S-
nitrosothiols. The dressing comprises one or more components containing a source of nitrite,
a source of thiol and a source of protons.
It is a well known fact mat the rate of the nitrosothiol generation can be controlled by pH. In
principle, the rate increases with increasing acidity of the formulation containing a source of
nitrite and a thiol. However, whilst it is possible to ensure such rapid generation of
nitrosothiols simply by adjusting pH, the acidity required may prevent applicability of such
formulation (for example when applied onto intact or wounded skin).
Whilst some applications may require only a slow rate of nitrosothiol generation, there are
other applications that benefit from a rapid burst of nitrosothiols. It is possible to ensure such
rapid generation of nitrosothiols simply by adjusting pH, but the acidity required may
prevent applicability of such formulation (for example when applied onto intact or wounded
skin).
Summary of the Invention
The present invention relates to a skin dressing adapted, on activation, to generate one or
more S-nitrosothiols by reaction between a thiol and a nitrite salt in the dressing, the skin
dressing comprising a source of Cu2+, Zn2+ and/or Fe2+ ions.
The invention is based on the surprising finding that the presence of cations of transition
metals, such as Cu , Zn or Fe , preferably Cu , can increase the rate of nitrosothiol
production in a system containing a source of nitrite and a thiol. This is achieved without any
changes of the pH of the formulation. This effect provides an alternative control over the rate
of nitrosothiol generation and allows a more rapid nitrosothiol generation to be achieved at
milder pH, which is less irritating and harmful to skin.
Whilst the effect of two metal cations (Hg+ and Cu4) on the rate of nitrosothiol
decomposition has been well established for several decades, the use of transition metals to
increase the rate of nitrosothiol production was not previously considered.
It will be appreciated that nitrite is a compound with a pK, of 3.4 (at 25 °C). Thus, nitrite
can act as a buffer in the system, capable of maintaining pH in the range of from 3 to 4.
After activating the dressing, e.g. by bringing two components together, at a skin site, the pH
will be such mat S-nitrosothiols begin to be generated. During this reaction nitrite will help
to maintain an acidic environment. However, its buffering capacity will reduce as the
reaction proceeds.
Preferably there are no additional buffers with a pKa of from 1 to 4 in the dressing so that the
pH of the dressing rises as the reaction proceeds, increasing the pH from an acidic value (e.g.
below 5, e.g. from 3 to 4) to a more neutral value (e.g. above 5, e.g. from 6 to 7).
Although the rise in pH will reduce and eventually may stop production of S-nitrosothiols,
the presence of a source of Cu2+, Zn2+ and/or Fe2+ ions, particularly Cu2+, accelerates
production of S-nitrosothiols, allowing production to continue at higher pH values.
For example, if rapid generation of nitric oxide is required in order to achieve a localised
vasodilatation and consequent increase of blood flow then the pH of the formulation
immediately after activation must be very low so that the nitrosylation of thiol proceeds very
readily. However, in the presence of cations of transition metals the same rapid rate of thiol
nitrosylation can be achieved at a milder pH.
The thiol may be preferably selected from the group consisting of 1-thioglycerol, 1-
thioglucose, methyl- or ethyl-ester of cysteine.
Other suitable thiols include glutathione (L-glutathione, as this is the physiologically
important version), cysteine, N-acetylcysteine, mercaptoethylamine, 3-mercaptopropanoic
acid.
The one or more S-nitrosothiols are generated by reacting together reagents in the dressing.
The dressing includes a nitrite, e.g. potassium nitrite, and a thiol, e.g. 1-thioglycerol that
react together in the dressing on activation to generate and release S-nitrosothiol.
The reagents are suitably provided in separate components of the dressing that are kept apart
prior to use. To activate the dressing, the two dressing components are brought into contact
(in the presence of a source of water and protons, if required), resulting in production in the
dressing of the S-nitrosothiol that is then typically released from the dressing. Preferably, the
two dressing components are packaged separately. Alternatively, they can be brought into
contact and packaged together in a sealed container so that they are ready for use as a one
component system when unpackaged.
Preferably the dressing comprises a first component comprising the thiol and a second
component comprising the nitrite.
In this two component arrangement, typically the first component is acidic, having a pH in
the range of from 2 to 5, preferably from 3 to 4. The second component may have a pH in
the range of from 5 to 12, preferably from 6 to 11 and more preferably from 7 to 10. A small
amount of buffer with a pKa. in the range of from 7 to 12 is optionally present in the second
component
Preferably the first component additionally comprises a buffer with a pKa of from 4.5 to 7.0,
preferably of from 5 to 6, most preferably about 5.5. As discussed above, the pH of the first
component preferably has a value of from 3 to 4. At this pH a very high proportion of the
buffer will be present in protonated form and can thus serve as a useful source (or reservoir)
ofprotons.
Since the buffering capacity of this buffer is minimal at pH between about 3 to 4, nitrite will
be a dominant buffer in the composition following activation. As the coversion of nitrite to
S-nitrosothiol proceeds, accompanied by consumption of protons, the buffering capacity of
nitrite will diminish and pH will increase. The buffering contribution of the source of
protons buffer (with e.g. pKa about 5.5) will be minimal in the initial stages, but it will
prevent the pH from rising too sharply above 4.5, where the conversion of nitrite to S-
nitrosothiol is rather inefficient The pH will only reach those levels if most nitrite is
converted, at which point tew pH is no longer required as the build-up of nitric oxide has
been achieved.
Thus, there is a co-operation between nitrite (pKa 3.4) and the source of protons buffer (pKa
about 5.5) in terms of proton exchange, ensuring an efficient conversion of nitrite whilst
maintaining mild pH environment.
Suitable amounts of the reagents can be readily determined to achieve desired rate and yield
of production of one or more S-nitrosothiols. In general, amounts of each reagent in the
range 0.1% - 5% (w/w), based on the dressing, are likely to be appropriate.
The or each dressing component may be in the form of a layer, e.g. in the form of a sheet,
slab or film, that may produce from, an amorphous material, not having any fixed form or
shape, that can be deformed and shaped in three dimensions, including being squeezed
through a nozzle.
The or each dressing component conveniently comprises a carrier or support, typically in the
form of a polymeric matrix. The or each component of the system can be in the form of
liquid, amorphous gel or in the form of a layer e.g. in the form of a sheet, slab or dry film. A
particularly convenient support comprises a polymer based on polyacrylic acid which
contains dissociable groups with pKa between 5 to 6.
The carrier or support conveniently comprises a hydrated hydrogel. A hydrated hydrogel
means one or more water-based or aqueous gels, in hydrated form. A hydrated hydrogel
thus includes a source of water, for activation of the dressing. A hydrated hydrogel can also
act to absorb water and other materials exuded from a wound site, enabling the dressing to
perform a valuable and useful function by removing such materials from a wound site. The
hydrated hydrogel also provides a source of moisture, mat can act in use to maintain a
wound site moist, aiding healing.
Suitable hydrated hydrogels are disclosed in WO 03/090800. The hydrated hydrogel
conveniently comprises hydrophilic polymer material. Suitable hydrophilic polymer
materials include polyacrylates and methacrylates, e.g. as supplied by First Water Ltd in the
form of proprietary hydrogels, including poly 2-acryiamido-2-methylpropane sulphonic acid
(poly-AMPS) and/or salts thereof (e.g. as described in WO 01/96422), polysaccharides e.g.
polysaccharide gums particularly xanthan gum (e.g. available under the Trade Mark Keltrol),
various sugars, polycarboxylic acids (e.g. available under the Trade Mark Gantrez AN-169
BF from ISP Europe), poly(methyl vinyl ether co-maleic anhydride) (e.g. available under the
Trade Mark Gantrez AN 139, having a molecular weight in the range 20,000 to 40,000),
polyvinyl pyrrolidone (e.g. in the form of commercially available grades known as PVP K-
30 and PVP K-90), polyethylene oxide (e.g. available under the Trade Mark Polyox WSR-
301), polyvinyl alcohol (e.g. available under me Trade Mark Elvanol), cross-linked
polyacrylic polymer (e.g. available under the Trade Mark Carbopol EZ-1), celluloses and
modified celluloses including hydroxypropyl cellulose (e.g. available under the Trade Mark
Klucel EEF), sodium carboxymethyl cellulose (e.g. available under the Trade Mark
Cellulose Gum 7LF) and hydroxyethyl cellulose (e.g. available under the Trade Mark
Natrosol 250 LR).
Mixtures of hydrophilic polymer materials may be used in a gel.
In a hydrated hydrogel of hydrophilic polymer material, the hydrophilic polymer material is
desirably present at a concentration of at least 1%, preferably at least 2%, more preferably at
least 5%, yet more preferably at least 10%, or at least 20%, desirably at least 25% and even
more desirably at least 30% by weight based on the total weight of the gel. Even higher
amounts, up to about 40% by weight based on the total weight of the gel, may be used.
Good results have been obtained with use of a hydrated hydrogel of poly-AMPS and/or salts
thereof in an amount of about 30% by weight of the total weight of the gel.
The hydrated hydrogel material is typically in the form of a solid layer, sheet or film of
material that is typically cross-linked, and that may incorporate a mechanical reinforcing
structure. The size and shape of the layer, sheet or film can be selected to suit the intended
use of the dressing. Thicknesses in the range 0.05 to 5 mm, preferably 0.5 to 3 mm are
particularly suitable.
Alternatively, the hydrated hydrogel may be in the form of an amorphous gel not having a
fixed form or shape, that can be deformed and shaped in three dimensions, including being
squeezed through a nozzle. Amorphous gels are typically not cross-linked or have low
levels of cross-linking. A shear-thinning amorphous gel may be used. Such a gel is liquid
when subjected to shear stress (e.g. when being poured or squeezed through a nozzle) but set
when static. Thus the gel may be in the form of a pourable or squeezable component that
may be dispensed, e.g. from a compressible tube or a syringe-like dispenser, comprising a
piston and cylinder, typically with a nozzle of about 3 mm diameter. Amorphous gels allow
efficient mixing of the two-component system. Such a gel may be applied in the form of a
surface layer, or into a wound cavity as a fully conformable gel that fills the available space
and contacts the wound surface.
A typical example of an amorphous gel formulation is: 15% w/w AMPS (sodium salt),
0.19% polyethylene glycol diacrylate and 0.01% hydroxycyclohexyl phenyl ketone, with the
volume made up to 100% with analytical grade DI water. The reagents are thoroughly
mixed and dissolved, then polymerised for between 30-60 seconds, using a UV-A lamp
delivering approximately 100 mW/cm2, to form the required hydrogel. This may be
contained in plastic syringes from which the amorphous gel may then be dispensed from a
syringe to a target site, as a surface layer or to fill a cavity.
The components of the dressing can also be in the dry form. Examples of suitable dry
support polymer materials comprise polyvinylalcohol (PVA), alginate or
carboxymethylcellulose. The stability of the active substances, especially thiols, will
generally be better in the dry form.
In one embodiment the invention comprises a first component comprising a layer of
hydrated hydrogel, preferably poly-AMPS and/or salts thereof, containing a source of nitrite,
e.g. potassium nitrite, and a second component comprising a dry polymeric matrix,
preferably dried PVA, containing a thiol, e.g. 1-thioglyceroL The first component may be
used in contact with the skin, as the hydrated hydrogel has beneficial properties for skin
contact, as discussed above, with the second component being placed on top of the first
component. When the two components are brought into contact, this has the effect of
activating the dressing. The water in the hydrated hydrogel of the first component functions
to provide a suitable aqueous environment allowing generation of S-nitrosothioL
In another embodiment, the dressing comprises two components which are amorphous. The
components can be in the form of e.g. a gel, semi-solid, paste, cream, lotion or liquid e.g. an
aqueous solution. Hydrated hydrogels may be conveniently employed, as discussed above.
In embodiments of this type, each component preferably contains a reagent which, when
brought together, activate to release one or more S-nitrosothiols. Preferably one component
contains a nitrite and the other contains a thiol. Alternatively the nitrite and thiol could be
kept together at a high enough pH to prevent reaction thereof, e.g. at a pH above 7, the
second component containing a source of acidity. Another possibility is that one component
contains anhydrous S-nitrosothiol and the second component contains water.
The two amorphous components are kept separate until it is desired to apply the dressing to a
body surface. Conveniently they are packaged in a container having a nozzle, through which
the amorphous components can be delivered. Preferably, the two components are packaged
in a two compartment dispenser, preferably being operable to deliver both components
simultaneously. The two components can also be brought into contact and packaged together
in a sealed container so that they are ready for use as a one component system when
unpackaged.
In yet another embodiment the dressing comprises two dry components. Examples of
suitable dry support polymer materials comprise polyvinylalcohol (PVA), alginate or
carboxymethylcellulose. The two components can either be kept separate during storage and
activated by bringing them together and adding moisture. Alternatively, they can be kept
together during storage and be activated by adding moisture.
Preferred embodiments comprise two dressing components, one containing nitrite and the
other containing thiol, e.g. glutathione. The two components can take a wide variety of
material forms, as discussed above. However, the following examples of combinations are
currently preferred:
The dressing optionally includes, or is used with, a skin contact layer, preferably comprising
a hydrated hydrogel of poly-AMPS and/or salts thereof, as mentioned above.
The dressing optionally includes, or is used with, a covering or outer layer for adhering the
dressing to the skin of a human or animal in known manner.
Dressings in accordance with the invention can be manufactured in a range of different sizes
and shapes for treatment of areas of skin e.g. wounds of different sizes and shapes.
Appropriate amounts of reagents for a particular dressing can be readily determined by
experiment.
Dressing components are suitably stored prior to use in sterile, sealed, water-impervious
packages, e.g. dual chamber plastic tubes or laminated aluminium foil packages.
In use, the dressing component or components are removed from their packaging and located
in appropriate order on the skin of a human or animal, e.g. over a wound or other region of
skin to be treated for cosmetic or therapeutic purposes. The dressing may also be used as an
adjuvant for transdermal delivery, as noted above. The dressing is activated, in the case of
multiple component dressings, by bringing the components into contact, resulting in release
from the dressing of one or more S-nitrosothiols (after generation in the dressing after
activation). S-nitrosothiols decompose spontaneously to produce nitric oxide, which has
beneficial effects on tissues and also causes vasodilation.
The invention will be further described, by way of illustration, in the following Examples,
and with reference to the accompanying figures, in which:
Figure 1 is a graph of absorbance (338 nm) versus time (in minutes) showing the effect of
cupric cations on the rate of nitrosylation of 1-thioglycerol at pH 4.0. The absorbance (338
nm) is directly proportional to the concentration of S-nitroso-1-thioglycerol in the solution.
Figure 2 is a graph of absorbance (338 nm) versus time (in minutes) showing the effect of
cupric cations on the rate of nitrosylation of 1-thioglycerol at pH 4.5. The absorbance (338
nm) is directly proportional to the concentration of S-nitroso-1-thioglycerol in the solution.

Figure 3 is a graph of absorbance (338 ran) versus time (in minutes) showing the effect of
cupric cations on the rate of nitrosylation of 1-thioglycerol at pH 5.0. The absorbance (338
nm) is directly proportional to the concentration of S-nitroso-1-thioglycerol in the solution.
Figure 4 is a graph of absorbance (338 nm) versus time (in minutes) showing the effect of
zinc cations on the rate of nitrosylation of 1-thioglycerol at pH 4.0. The absorbance (338 nm)
is directly proportional to the concentration of S-nitroso-1-thioglycerol in the solution.
Figure 5 is a graph of absorbance (496 nm) versus time (in minutes) showing the effect of
ferrous cations on the rate of nitrosylation of 1-thiogrycerol at pH 4.0. The absorbance (496
nm) is directly proportional to the concentration of S-nitroso-1-thioglycerol in the solution.
Figure 6 is a graph of absorbance (496 nm) versus time (in minutes) showing the effect of
ferrous cations on the rate of nitrosylation of 1-thioglycerol at pH 4.5. The absorbance (496
nm) is directly proportional to the concentration of S-nitroso- 1-thioglycerol in the solution.
Examples
Materials and Methods
Chemicals & other materials
Water (conductivity < 10 uS cm"1; either analytical reagent grade, Fisher or Sanyo Fistreem
MultiPure)
Sodium nitrite, from Sigma (S2252)
1-Thioglycerol, fromFluka (88641)
Hydrochloric acid, from Fisher (J/4310/17)
Ferrous sulphate, from Aldrich (21,542-2)
Cupric nitrate, from Aldrich (467855)
Zinc sulphate, from Sigma (Z4750)
Sodium citrate, from Fisher (BPE327-500)
Measurement of S-nitrosotfaiol concentration in aqueous solutions using direct absorbance
measurement at 338 nm
Nitrosothiols are known to absorb UV light around 338 nm (8338 = approximately 900 M'1
cm'1). The nitrosylation rate can therefore be followed by direct absorbance measurement at
338 nm (Cook et aL Analytical Biochemistry, 238, 150-158, 1996). A solution containing
nitrite (5 mM) and a given concentration of transition metal (either Cu2+ or Zn2+) was
prepared in citrate buffer (50 mM, either pH 4.0 or pH 4.5) and absorbance (338 nm) was
measured. 1-Thioglycerol was added to the mixture to achieve 5 mM concentration and
changes in absorbance (338 nm) were followed as a function of time. Due to interference
from iron species this method could not be used when studying the effect of Fe2+ cations on
the rate of nitrosylation rate.
Measurement of S-nitrosothiol concentration in aqueous solutions using the Griess method
This method was used when studying the effect of Fe2+ cations on the rate of nitrosylation
rate. A solution containing nitrite (5 mM) and a given concentration of Fe2+ was prepared in
citrate buffer (either pH 4.0 or pH 4.5 or pH 5.0). 1-Thioglycerol was added to the mixture to
achieve 5 mM concentration and the concentration of S-nitroso-1-thioglycerol was measured
as a function of time using the following procedure (based on Cook et al. Analytical
Biochemistry, 238,150-158,1996):
The following reagents were prepared:
Reagent 1: Na-phosphate buffer (pH 7.4, 0.1 M)
Reagent 2: Griess reagent: 20 mg of N-(1-Naphthyl)ethylendiamine dihydrochloride
(NADD) + 500 mg of sulphanilamide dissolved in 2 mL of DMSO. (KB. This solution is
light sensitive and should be kept in the dark as much as possible)
Reagent 3: Mercuric chloride (10 mM) in DMSO (13.58 mg of HgCl2 in 5 mL of
DMSO)
The six-step procedure set out below was then followed:
Dispense 1.5 mL of Reagent 1 into aplastic cuvette
Add 200 uL of the sample (i.e. sample in which S-nitroso-1-thioglycerol concentration is to
be determined)
Add 1.17 mL of DI water
Add 100 uL of Reagent 2
Add 30 uL of Reagent 3 and give the solution a good mix
Read absorbance of the resulting mixture at 496 nm in 10 min.
The concentration of nitrosothiol concentration can be estimateded from the absorbance
reading using the molar absorption coefficient for nitrosotbiols = approximately 10,000 M"1
cm"1.
Example 1: Effect of Cu2+ on the rate of nitrosvlation of 1-thioelvcerol
This example demonstrates the effect of cupric cations on the the rate of nitrosothiol
generation at pH 4.0 (Fig. 1), pH 4.5 (Fig. 2) and pH 5.0 (Fig. 3). The rate of nitrosvlation,
followed by measuring absorbance of the samples at 338 nm, increased considerably in the
presence of cupric cations in the concentration range 10 uM to 100 uM.
Example 2: Effect of Zn2* on the rate of nitrosvlation of 1 -thioglvcerol
This example demonstrates the effect of zinc cations on the the rate of nitrosothiol
generation at pH 4.0 (Fig. 4). The rate of nitrosylation, followed by measuring absorbance of
the samples at 338 nm, increased in the presence of 100 uM zinc cations. The increase in
nitrosylation rate was more marked in the presence of 250 uM zinc cations.
Example 3: Effect of Fe2+ on the rate of nitrosvlation of 1-thioglvcerol
This example demonstrates the effect of ferrous cations on the the rate of nitrosothiol
generation at pH 4.0 (Fig. 5) and pH 4.5 (Fig. 6). The rate of nitrosylation could not be
followed by measuring absorbance of the samples at 338 nm due to interference from the
iron species. Instead, the generation of the S-nitroso-1-thioglycerol was followed by the
Griess method (Cook et al. Analytical Biochemistry, 238, 150-158, 1996). The nitrosylation
rate was found to increase in the presence of both 100 uM and 250 uM ferrous cations.
Claims
1. A skin dressing adapted, on activation, to generate one or more S-nitrosothiols by
reaction between a thiol and a nitrite salt in the dressing, the skin dressing comprising a
source of Cu2+, Zn2+ and/or Fe2+ ions.
2. A skin dressing according to claim 1, comprising a first component comprising the
thiol and a second component comprising the nitrite salt.
3. A skin dressing according to claim 1 or claim 2, which is free of any additional
materials having a pK, of from 1.0 to 4.0.
4. A skin dressing according to any one preceding claim, wherein, as the nitrite and
thiol react to generate the one or more S-nitrosothiols, the pH of the dressing in contact with
the skin increases from an acidic value to a more neutral value.
5. A skin dressing according to any one preceding claim, which comprises a buffer with
a pKa of from 4.5 to 7.0.
6. A skin dressing according to any one of claims 2 to 5, wherein the pH of the first
component is from 2.0 to 5.0.
7. A skin dressing according to any one of claims 2 to 6, wherein the pH of the second
component is from 5.0 to 12.0.
8. A skin dressing according to any one of claims 2 to 7, wherein the first and second
components are amorphous.
9. A skin dressing according to any one of claims 2 to 8 wherein the first component
and/or the second component comprise a polymeric support.
10. A skin dressing according to claim 9, wherein the polymeric support comprises
polyacrylic acid

Improvements relating to skin dressing A skin dressing adapted, on activation, to generate one or more S-nitrosoth-
iols by reaction between a thiol and a nitrite salt in the dressing, the skin dressing comprising a source of Cu ,Zn and/or Fe ions
for delivery of nitric oxide to a body site is provided.