Description:FIELD OF THE INVENTION
The present invention relates to the field of pesticides. The present invention in particular relates to a synergistic, broad spectrum insecticidal composition comprising Emamectin benzoate, Lambda-cyhalothrin and Bifenthrin. The present invention further relates to the process of preparation of said composition and uses thereof.
BACKGROUND OF THE INVENTION
Protection of crops and its produce from insect pest damage is essential for enhancement of agriculture produce. Even with the availability of alternative types of pest control, chemical control remains an important tool for the prevention and control of pests in agriculture, especially insect pests. Insecticides of varied types and structural groups have been reported in literature and a large number are in commercial use. However, judicious use of such chemical pesticides is absolutely needed to protect the environment and natural ecosystems. In this regard, application of insecticidal combinations has emerged as an attractive approach to ensure that the chemical load in the environment is reduced. However, not all chemical insecticidal actives are compatible with each other, and therefore requires a great amount of experimentation and effort to develop an efficacious pesticidal combination, which is stable and effective in controlling the insect pests in economically important agricultural crops.
CN 102037994 A enables the combination of Phenthoate (Cidial) and Emamectin benzoate emulsifiable concentrates formulation comprising solvents and surfactants.
WO 2000/002453 A1 generically enables the combination of Phenthoate and Emamectin benzoate emulsifiable concentrates formulation comprising use of surfactants, and an aromatic solvent.
There is therefore a continued need to develop insecticidal compositions that provide multiple advantages including to enable increase in the crop yields, to provide a composition with high synergistic action, to provide a combination where the dosage of the active ingredients is reduced as compared to the individual recommended dosage, to avoid excess loading of the toxicant to the environment and to negligibly impact environmental safety. The present invention aims to provide such an insecticidal composition with aforesaid advantageous features. More importantly, the present invention aims at providing a composition wherein the insecticidal activity of the insecticidal composition is considerably higher than the sum of the activities of the individual active compounds and that results into good enhancement in crop yield.
OBJECTS OF THE INVENTION
It is an object of the present invention to provide a novel, stable, synergistic, insecticidal composition comprising Emamectin benzoate, Lambda cyhalothrin and Bifenthrin as its ingredients.
It is another object of the present invention to provide an insecticidal composition comprising Emamectin benzoate and Lambda cyhalothrin as insecticidal active ingredients and Bifenthrin as a synergist.
It is yet another object of the present invention to provide an insecticidal composition with enhanced bioefficacy at reduced dosage as compared to individual insecticides when used in isolation.
It is yet another object of the present invention to provide an insecticidal composition that results in good enhancement in crop yield.
It is yet another object of the present invention to provide an insecticidal composition that is environmentally safe and non-phytotoxic.
It is yet another object of the present invention to provide an insecticidal composition that has broad spectrum activity and can be used in a wide variety of agricultural crops.
It is yet another object the present invention is to provide emulsifiable concentrate composition comprising combination of Emamectin, lambda cyhalothrin and Bifenthrin or its salt, surfactants, hydrocarbon solvent, co-solvent and optionally other agrochemical auxiliaries.

SUMMARY OF THE INVENTION
Accordingly, in one aspect, the present invention provides a novel, stable,synergistic, environmentally safe, broad spectrum, cost effective insecticidal composition comprising Emamectin benzoate as the first active ingredient, lambda cyhalothrin as the second active ingredient and Bifenthrin as the third active ingredient as a synergist.
In another aspect, the present invention provides a novel, stable, synergistic, environment friendly, broad spectrum, cost effective insecticidal composition that is effective in controlling Stem borer and Leaf folder insect-pests on Rice crop.
In another aspect, the present invention provides a stable formulation comprising said insecticidal composition. In a preferred aspect, said insecticidal composition is formulated as an Emulsion concentrate.
In yet another aspect, the present invention provides a process for preparing said insecticidal composition.

DETAILED DESCRIPTION OF THE INVENTION
Those skilled in the art will be aware that the invention described herein is subject to variations and modifications other than those specifically described. It is to be understood that the invention described herein includes all such variations and modifications. The invention also includes all such steps, features, compositions and compounds referred to or indicated in this specification, individually or collectively, and all combinations of any two or more of said steps or features.
Unless otherwise specified, all terms used in disclosing the invention, including technical and scientific terms, have the meaning as commonly understood by one of the ordinary skill in the art to which the invention belongs. For further guidance, term definitions may be included to better appreciate the teaching of the present invention.
As used herein, the term “plant” refers to any plant or part thereof including serial and subterranean parts of the plant. It is contemplated that the parts of the plant may be for example, flowers, fruits or vegetables, shoots, leaves, needles, stalks, stems, fruiting bodies, seeds also roots and that parts of 5 the plants may or may not be attached to the remainder of the plant.
The term “locus” of a plant as used herein is intended to embrace the place on which the plants are growing, where the plant propagation materials of the plants are sown or where the plant propagation materials of the plants will be placed into the soil.
The term “plant propagation material” is understood to denote generative parts of a plant, such as seeds, vegetative material such as cuttings or tubers, roots, fruits, tubers, bulbs, rhizomes and parts of plants, germinated plants and young plants which are to be transplanted after germination or after emergence from the soil. These young plants may be protected before transplantation by a total or partial treatment by immersion.
It is to be understood that the singular forms “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise.
It should be emphasized that the term “comprises/comprising” when used in this specification is taken to specify the presence of stated features, steps or components but does not preclude the presence or addition of one or more other features, steps, components or groups thereof.
The term "insects" as used herein, includes all organisms in the class "Insecta."
The term "Insecticidal" as used herein, refers to the ability of a pesticide to increase mortality or inhibit growth rate of insects.
The terms “weight percent”, “wt-%”, “percent by weight”, “% by weight” and variations thereof, as used herein, refer to the concentration of a substance as the weight of that substance divided by the total weight of the composition and multiplied by 100. It is understood that, as used here, “percent “, “%” and the like are intended to be synonymous with “weight percent”, “wt. %”, etc.
The term “synergist” as used herein refers to a material which enhances the activity of other materials such as insecticides so that the overall activity of the composition is greater than the sum of the individual ingredients.
Features that are described and/or illustrated with respect to one embodiment may be used in the same way or in a similar way in one or more other embodiments and/or in combination with or instead of the features of the other embodiments.

Emamectin benzoate chemically known as (4R)-4-deoxy-4-(methylamino)avermectin B1 benzoate (1:1) is an avermectin insecticide. It is a contact insecticide with negligible toxicity to non-target organisms. It allosterically activates glutamate-gated chloride channels (GluCls) causing paralysis in the insects
Lambda-cyhalothrin chemically known as (R)-cyano-(3-phenoxyphenyl)methyl (1S,3S)-rel-3-[(1Z)-2-chloro-3,3,3-trifluoro-1-propen-1-yl]-2,2-dimethylcyclopropanecarboxylate is a pyrethroid ester insecticide. It is a contact insecticide and it acts by disrupting the gating mechanism of sodium channels that are involved in the generation and conduction of nerve impulses, causing rapid paralysis and death of the insects.
“Bifenthrin” means 2-methylbiphenyl-3-ylmethyl (Z)-(lRS)-cis-3-(2-chloro-3, 3, 3-trifluoroprop-l-enyl)-2, 2-dimethyl cyclopropanecarboxylate. Bifenthrin is effective against a wide spectrum of pests when ingested, but typically is more active than chlorantraniliprole through direct contact, thus functioning as a low to moderately effective ovicide and adulticide in addition to being a good larvicide.
Embodiments of the present invention provide insecticidal combination and composition comprising said combination that possess an enhanced efficacy over the individual active ingredient compounds, when used in isolation.
The present invention thus provides an insecticidal composition comprising a) Emamectin Benzoate as the first active ingredient b) Lambda cyhalothrin as the second active ingredient and c) Bifenthrin as the third active ingredients. Wherein Emamectin Benzoate is present in an amount ranging from 0.1-20% w/w, Lambda cyhalothrin is present in an amount ranging from 0.5-25% w/wand Bifenthrin is present in an amount ranging from 0.5-30% w/w the insecticidal composition.
In a preferred embodiment, Emamectin Benzoate is present in an amount 1.25%w/w, Lambda cyhalothrin is present in an amount of 1.5%w/w and Bifenthrin is present in an amount of 4.5% w/w of the insecticidal composition.
In an embodiment, the insecticidal composition of the present invention comprising the said actives exhibits synergistic effect in control of insects in agricultural crops, particularly arthropod insects in Rice crop, particularly Stem borer and Leaf folder.
In an embodiment, the insecticidal composition of the present invention may be applied to a variety of agricultural crops like brinjal, colecrops, cucurbits, cotton, tea, ornamentals, horticultural crops and oil seed crops.
In a more preferred embodiment, the insecticidal composition of the present invention is formulated in the form of a Emulsion concentrate comprising a surfactant, a solvents and co solvents.
In an embodiment, the at least a surfactant agent is selected from the group comprising 4-(5-dodecyl)benzenesulfonate, docusate (dioctylsodiumsulfosuccinate), alkyl ether phosphates, benzalkaonium chloride (BAC), and perfluorooctanesulfonate (PFOS), Blend of Ionic and Non inonicsurfactant.The at least a surfactant agent weight concentration in said Insecticidal composition is in the range of -06 -20 % w/w.
In an embodiment, the atleast the hydrocarbon solvent as used herein is selected from and not limited to C1 to C5 alkyl groups, aliphatic or aromatic hydrocarbons, paraffins or toluene, xylenes and naphthalene derivatives, in particular 1-methyl-naphthalene, 2-methylnaphthalene, mixtures of C6-C16-aromatic compounds, C9-solvent, cyclohexanol, butanol, methyl ethyl ketone, isopropanol. Preferred hydrocarbon solvent used in the composition of the present invention is C9-solvent.The at least a solvent weight concentration in said Insecticidal composition is in the range of 1-5% w/w.
In an embodiment.the at least co solvent is selected from the group consisting of NMP, cyclohexane,dimethylformamide, isophoron and N-methylpyrrolidon.The at least a solvent weight concentration in said Insecticidal composition is in the range of 05-90% w/w.
In an embodiment, the insecticidal composition is formulated in a form selected from the group comprising water-soluble concentrates (SL), emulsifiable concentrates (EC), emulsions (EW), micro-emulsions (ME), Suspension concentrates (SC), oil-based suspension concentrates (OD), flowable suspensions (FS), water-dispersible granules (WG), water-soluble granules (SG), wettable powders (WP), water soluble powders (SP), granules (GR), encapsulated granules (CG), fine granules (FG), macrogranules (GG), dry flowables (DF), aqueous Suspo-emulsions (SE), capsule suspensions (CS) and microgranules (MG). In a preferred embodiment, the Insecticidal composition is in the form of suspension concentrates (SC), water-dispersible granule (WDG) and wettable powder (WP).
In a preferred embodiment, the Insecticidal composition of the present invention is formulated as an Emulsion concentrate.

Examples-1:
The examples below are given solely for the purpose of illustration and are not to be construed as limitations of the present invention, as many variations thereof are possible without departing from the spirit and scope of the invention.
Example 2: Preparation of Insecticidal composition as Emulsion concentrates (EC).
In an embodiment, the chemical composition of the present insecticidal is depicted below in Table 1 (a) below:

Table-1 (a): Composition of the Insecticidal composition
S.no. Ingredients Percentage range
1 Emamectin Benzoate technical (Basis of 100.0%) 0.1-20% w/w
2 Lambda Cyhalothrin technical (Basis of 100.0%) 0.5-25% w/w
3 Bifenthrin Technical (Basis of 100.0%) 0.5-30% w/w
4 Blend of ionic and non-ionic emulsifier 06-20 % w/w
5 NMP Co-solvent 05-25% w/w
6 Cyclohexane Co-solvent 05-20.0% w/w
7 C-9 Solvent 05-90% w/w

In a preferred embodiment, the chemical composition of the present insecticidal composition is depicted below in Table 1(b)
Table-1 (b): Preferred composition of the Insecticidal composition.
Sno Ingredients Percentage
%w/w

1 Emamectin Benzoate technical (Basis of 100.0%) 1.25% w/w
2 Lambda Cyhalothrin technical (Basis of 100.0%) 1.5% w/w
3 Bifenthrin Technical (Basis of 100.0%) 4.5% w/w
4 Blend of ionic and non-ionic emulsifier 12% w/w
5 NMP Co-solvent 10% w/w
6 Cyclohexane Co-solvent 20% w/w
7 C-9 Solvent QS
Total Quantity 100%

Preparation method of Emulsion concentrates:
(a) Adding and mixing of NMP and Emamectin benzoate and mixed it for half an hour to obtain pre-mix.
(b) Adding cyclohexane into the premix and stir @ 1500RPM for another half an hour.
(c) Adding lambda cyhalothrin and Bifenthrin one by one to the reaction mixture followed by adding C-9 solvent under continuous stirring it for 1 hour.
(d) Adding blend of emulsifier in appropriate quantity and mix it for half an hour to get clear solution.

Table-1(c): Quality parameters of EC formulation
Parameter Desired quality
Description/ physical appearance Material shall be in the form of white to free flowing suspension
Emulsion stability shall not be less 5 than 50% w/w
Persistence of foam Not more than 60ml in 12 min
Water content 0.2% max.
pH of 1% aq. Solution 4.0- 9.0
Heat stability test Take 100ml sample & keep it in 54°C±2 for 14 days check for all parameters listed above thereafter
Cold stability test Take 100ml sample & keep it in 5°C±2 for 7 days, check for all parameters listed above thereafter.

Example-3: Field evaluation of the bio-efficacy of the present Insecticidal composition.

The presently disclosed insecticidal tri-mix was tested for its bioefficacy against Stem borer and Leaf folder on Rice crop. The plants were aligned in a plot size of 25 square meter. The trial was carried out in a Pusa-1509 variety of Rice in karnal, Haryana. The trial was laid out in a random block design consisting of a total of 13 treatments in three replications.The1st application just after initiation of insects at ETL level (35-40 DAT of rice) and followed by 15 days interval.

Measured quantity of the chemical was added to required volume of water @ 500L/ha for spraying. The spray tank was filled with ½ the quantity of clean required volume of water and then the measured chemical (according to the dose) was added followed by the rest half quantity of water. The solution was stirred well and thorough coverage were ensured.

Table-2: below provides the treatment details in Rice crop.
Treatments Treatment details Dose (g a.i./ha) Dose (Formulation/ha)
T-1 Untreated - -
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml
T-4 Bifenthrin 10 % EC 50 500 ml
T-5 Emamectin Benzoate 01.90% +Lambda-cyhalothrin 5% +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml
T-6 Emamectin Benzoate 1.90% + Lambda-cyhalothrin 5 % +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml
T-7 Emamectin Benzoate 1.90% + Lambda-cyhalothrin 5 % +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml
T-8 Emamectin Benzoate 1.90% + Lambda-cyhalothrin 5 % +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml
T-9 Emamectin Benzoate 1.90% + Lambda-cyhalothrin 5 % +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml
T-10 Emamectin Benzoate 1.90% + Lambda-cyhalothrin 5 % +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml
T-11 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 7.031 + 8.438 + 25.312 562.5 ml
T-12 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 9.375 + 11.25 + 33.75 750 ml
T-13 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 11.719 + 14.062 + 42.187 937.5 ml

EVALUATION OF BIO-EFFICACY IN RICE CROP
Evaluation of bio-efficacyfor Stem borer & Leaf folder.

Stem borer: The efficacy of various insecticides against rice stem borer was judged on the basis of the percent dead heart at early stage and white ear heads at grain filling stage. The percent incidence of dead heart/white earheads was calculated as follows:

Percent incidence =(Number of dead heart/white ears )/(Total number of tillers/ panicles) x100

The observations on the dead hearts (DH) and white earheads (WEH) were recorded on ten hills selected randomly for each plot and tagged. Dead hearts were recorded at 0, 5, 10 and 15 days after application (DAA). While white earheads were recorded 10 days before crop maturity (10 DBCM).

Rice Leaf folder: To study the efficacy of insecticide, pest population was recorded in different phases i.e. before and after application. The damaged leaves and total leaves from 10 randomly selected hills were observed in each plot at 0,5, 10, 15 days after application (DAA). The percent incidence of leaf damage (LD) was calculated as follows:
Percent incidence =(Number of damaged leaves)/(Total number of leaves) x100

Table-3: Bio-efficacy of Emamectin Benzoate 1.25 % + Lambda-cyhalothrin 1.5 % +Bifenthrin 4.5 % EC against Stem borer (After First Application).
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) DH-0 DAA1 DH-5 DAA1 %
Incidence DH-10 DAA1 %
Incidence DH-15 DAA1 %
Incidence
T-1 Untreated - - 4 12 14.58 23 27.94 31 37.66
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 5 6 7.29 11 13.36 19 23.08
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 5 8 9.72 14 17.01 23 27.94
T-4 Bifenthrin 10 % EC 50 500 ml 4 8 9.72 14 17.01 22 26.73
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 5 6 7.29 6 7.29 6 7.29
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 4 5 6.07 5 6.07 5 6.07
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 4 5 6.07 5 6.07 5 6.07
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 5 6 7.29 6 7.29 6 7.29
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 4 5 6.07 5 6.07 5 6.07
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 5 5 6.07 5 6.07 5 6.07
T-11 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 7.031 + 8.438 + 25.312 562.5 ml 4 7 8.50 8 9.72 9 10.93
T-12 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 9.375 + 11.25 + 33.75 750 ml 5 5 6.07 5 6.07 5 6.07
T-13 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 11.719 + 14.062 + 42.187 937.5 ml 5 5 6.07 5 6.07 5 6.07

Table-4: Bio-efficacy of EmamectinBenzoate1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC against Stem borer (After Second Application)
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) DH-0 DAA2 DH-5 DAA2 %
Incidence DH-10 DAA2 %
Incidence DH-15 DAA2 %
Incidence WEH-10 DBCM %
Incidence
T-1 Untreated - - 31 33 40.09 34 41.31 37 44.95 16 37.21
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 19 20 24.30 22 26.73 22 26.73 6 10.53
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 23 26 31.59 27 32.80 29 35.23 9 17.65
T-4 Bifenthrin 10 % EC 50 500 ml 22 25 30.37 26 31.59 29 35.23 9 17.65
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 6 6 7.29 6 7.29 6 7.29 4 5.48
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 5 5 6.07 5 6.07 5 6.07 3 4.05
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 5 5 6.07 5 6.07 5 6.07 3 4.05
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 6 6 7.29 6 7.29 6 7.29 4 5.48
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 5 5 6.07 5 6.07 5 6.07 3 4.05
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 5 5 6.07 5 6.07 5 6.07 3 4.05
T-11 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 7.031 + 8.438 + 25.312 562.5 ml 9 10 12.15 10 12.15 11 13.36 12 16.67
T-12 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 9.375 + 11.25 + 33.75 750 ml 5 5 6.07 5 6.07 5 6.07 3 4.05
T-13 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 11.719 + 14.062 + 42.187 937.5 ml 5 5 6.07 5 6.07 5 6.07 3 4.05

Table-5: Bio-efficacy of EmamectinBenzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % ECagainst Leaf folder (After First Application)
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) LD-0 DAA1 LD-5 DAA1 %
Incidence LD-10 DAA1 %
Incidence LD-15 DAA1 %
Incidence
T-1 Untreated - - 4 12 14.58 23 27.94 31 37.66
T-1 Untreated - - 10 59 15.73 103 27.47 133 35.47
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 11 26 6.93 39 10.40 59 15.73
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 10 27 7.20 41 10.93 61 16.27
T-4 Bifenthrin 10 % EC 50 500 ml 11 26 6.93 40 10.67 60 16.00
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 10 11 2.93 12 3.20 13 3.47
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 11 11 2.93 11 2.93 11 2.93
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 10 10 2.67 10 2.67 10 2.67
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 10 12 3.20 12 3.20 13 3.47
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 11 11 2.93 11 2.93 11 2.93
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 10 10 2.67 10 2.67 10 2.67
T-11 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 7.031 + 8.438 + 25.312 562.5 ml 11 21 5.60 26 6.93 29 7.73
T-12 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 9.375 + 11.25 + 33.75 750 ml 10 11 2.93 11 2.93 11 2.93
T-13 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 11.719 + 14.062 + 42.187 937.5 ml 11 11 2.93 11 2.93 11 2.93

Table-6: Bio-efficacy of EmamectinBenzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC against Leaf folder(After Second Application)
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) LD-0 DAA2 LD-5 DAA2 %
Incidence LD-10 DAA2 %
Incidence LD-15 DAA2 %
Incidence
T-1 Untreated - - 133 151 40.27 159 42.40 173 46.13
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 59 61 16.27 63 16.80 67 17.87
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 61 63 16.80 65 17.33 69 18.40
T-4 Bifenthrin 10 % EC 50 500 ml 60 62 16.53 64 17.07 66 17.60
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 13 13 3.47 13 3.47 13 3.47
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 11 11 2.93 11 2.93 11 2.93
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 10 10 2.67 10 2.67 10 2.67
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 13 13 3.47 13 3.47 13 3.47
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 11 11 2.93 11 2.93 11 2.93
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 10 10 2.67 10 2.67 10 2.67
T-11 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 7.031 + 8.438 + 25.312 562.5 ml 29 31 8.27 33 8.80 37 9.87
T-12 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 9.375 + 11.25 + 33.75 750 ml 11 11 2.93 11 2.93 11 2.93
T-13 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 11.719 + 14.062 + 42.187 937.5 ml 11 11 2.93 11 2.93 11 2.93

Example-4: Evaluation of phytotoxicity of the present Insecticidal composition
Visual observations were recorded at 3, 7 and 10 days after application (DAA) of testing products. The parameters observed were leaf injury on tip/surface, necrosis, vein clearing, epinasty, hyponasty and wilting. For the rating on phytotoxicity the core Scale of 0 – 10 was followed for leaf injury on 5 tips/surface.

Table 7: Phytotoxicity symptoms scoring and rating for leaf injury on tip/surface

Leaf injury on tips/surface Rating
0% 0
1-10% 1
11-20% 2
21-30% 3
31-40% 4
41-50% 5
51-60% 6
61-70% 7
71-80% 8
81-90% 9
91-100% 10

Table-8: PhytotoxicityofEmamectinBenzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC against Stem borer(After First Application)
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) 12 Hours AA1 1 DAA1 3 DAA1 5 DAA1 7 DAA1 10 DAA1
T-1 Untreated - - 0 0 0 0 0 0
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 0 0 0 0 0 0
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 0 0 0 0 0 0
T-4 Bifenthrin 10 % EC 50 500 ml 0 0 0 0 0 0
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 0 0 1 3 3 3
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 0 0 1 4 5 5
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 0 0 1 5 6 6
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 0 0 0 0 0 0
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 0 0 0 0 0 0
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 0 0 0 0 0 0
T-11 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 7.031 + 8.438 + 25.312 562.5 ml 0 0 0 0 0 0
T-12 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 9.375 + 11.25 + 33.75 750 ml 0 0 0 0 0 0
T-13 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 11.719 + 14.062 + 42.187 937.5 ml 0 0 0 0 0 0

Table-9: PhytotoxicityofEmamectinBenzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC against Stem borer (After Second Application)
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) 12 Hours AA2 1 DAA2 3 DAA2 5 DAA2 7 DAA2 10 DAA2
T-1 Untreated - - 0 0 0 0 0 0
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 0 0 0 0 0 0
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 0 0 0 0 0 0
T-4 Bifenthrin 10 % EC 50 500 ml 0 0 0 0 0 0
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 0 0 1 3 3 3
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 0 0 1 4 5 5
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 0 0 1 5 6 6
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 0 0 0 0 0 0
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 0 0 0 0 0 0
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 0 0 0 0 0 0
T-11 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 7.031 + 8.438 + 25.312 562.5 ml 0 0 0 0 0 0
T-12 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 9.375 + 11.25 + 33.75 750 ml 0 0 0 0 0 0
T-13 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 11.719 + 14.062 + 42.187 937.5 ml 0 0 0 0 0 0

Table-10: Phytotoxicity of EmamectinBenzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC against Leaf folder (After First Application)
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) 12 Hours AA1 1 DAA1 3 DAA1 5 DAA1 7 DAA1 10 DAA1
T-1 Untreated - - 0 0 0 0 0 0
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 0 0 0 0 0 0
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 0 0 0 0 0 0
T-4 Bifenthrin 10 % EC 50 500 ml 0 0 0 0 0 0
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 0 0 1 3 3 3
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 0 0 1 4 5 5
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 0 0 1 5 6 6
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 0 0 0 0 0 0
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 0 0 0 0 0 0
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 0 0 0 0 0 0
T-11 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 7.031 + 8.438 + 25.312 562.5 ml 0 0 0 0 0 0
T-12 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 9.375 + 11.25 + 33.75 750 ml 0 0 0 0 0 0
T-13 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 11.719 + 14.062 + 42.187 937.5 ml 0 0 0 0 0 0

Table-11: Phytotoxicity of EmamectinBenzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC against Leaf folder (After Second Application)
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) 12 Hours AA2 1 DAA2 3 DAA2 5 DAA2 7 DAA2 10 DAA2
T-1 Untreated - - 0 0 0 0 0 0
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 0 0 0 0 0 0
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 0 0 0 0 0 0
T-4 Bifenthrin 10 % EC 50 500 ml 0 0 0 0 0 0
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 0 0 1 3 3 3
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 0 0 1 4 5 5
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 0 0 1 5 6 6
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 0 0 0 0 0 0
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 0 0 0 0 0 0
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 0 0 0 0 0 0
T-11 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 7.031 + 8.438 + 25.312 562.5 ml 0 0 0 0 0 0
T-12 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 9.375 + 11.25 + 33.75 750 ml 0 0 0 0 0 0
T-13 Emamectin benzoate 1.25 % + Lambda-cyhalothrin 1.5 % + Bifenthrin 4.5 % EC 11.719 + 14.062 + 42.187 937.5 ml 0 0 0 0 0 0

Table-12: Bio-efficacy of Emamectin benzoate 1.0 % + Lambda-cyhalothrin2.0 % + Bifenthrin 3.0 % EC against Stem borer (After First Application).
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) DH-0 DAA1 DH-5 DAA1 %
Incidence DH-10 DAA1 %
Incidence DH-15 DAA1 %
Incidence
T-1 Untreated - - 4 12 14.58 23 27.94 31 37.66
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 5 6 7.29 11 13.36 19 23.08
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 5 8 9.72 14 17.01 23 27.94
T-4 Bifenthrin 10 % EC 50 500 ml 4 8 9.72 14 17.01 22 26.73
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 5 6 7.29 6 7.29 6 7.29
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 4 5 6.07 5 6.07 5 6.07
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 4 5 6.07 5 6.07 5 6.07
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 5 6 7.29 6 7.29 6 7.29
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 4 5 6.07 5 6.07 5 6.07
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 5 5 6.07 5 6.07 5 6.07
T-11 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 5.62 + 11.25 + 16.875 562.5 ml 4 8 9.72 9 10.93 10 12.15
T-12 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 7.5 + 15 + 22.5 750 ml 5 6 7.29 7 8.50 8 9.72
T-13 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 9.375 + 18.75 + 28.125 937.5 ml 5 6 7.29 7 8.50 8 9.72

Table-13: Bio-efficacy of Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC against Stem borer (After Second Application).
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) DH-0 DAA2 DH-5 DAA2 %
Incidence DH-10 DAA2 %
Incidence DH-15 DAA2 %
Incidence WEH-10 DBCM %
Incidence
T-1 Untreated - - 31 33 40.09 34 41.31 37 44.95 16 37.21
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 19 20 24.30 22 26.73 22 26.73 6 10.53
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 23 26 31.59 27 32.80 29 35.23 9 17.65
T-4 Bifenthrin 10 % EC 50 500 ml 22 25 30.37 26 31.59 29 35.23 9 17.65
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 6 6 7.29 6 7.29 6 7.29 4 5.48
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 5 5 6.07 5 6.07 5 6.07 3 4.05
T-7 EmamectinBenzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 5 5 6.07 5 6.07 5 6.07 3 4.05
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 6 6 7.29 6 7.29 6 7.29 4 5.48
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 5 5 6.07 5 6.07 5 6.07 3 4.05
T-10 EmamectinBenzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 5 5 6.07 5 6.07 5 6.07 3 4.05
T-11 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 5.62 + 11.25 + 16.875 562.5 ml 10 11 13.36 12 14.58 13 15.79 12 16.67
T-12 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 7.5 + 15 + 22.5 750 ml 8 9 10.93 10 12.15 10 12.15 5 6.76
T-13 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 9.375 + 18.75 + 28.125 937.5 ml 8 9 10.93 10 12.15 10 12.15 5 6.76

Table-14: Bio-efficacy of Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC against Leaf folder (After First application).
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) LD-0 DAA1 LD-5 DAA1 %
Incidence LD-10 DAA1 %
Incidence LD-15 DAA1 %
Incidence
T-1 Untreated - - 10 59 15.73 103 27.47 133 35.47
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 11 26 6.93 39 10.40 59 15.73
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 10 27 7.20 41 10.93 61 16.27
T-4 Bifenthrin 10 % EC 50 500 ml 11 26 6.93 40 10.67 60 16.00
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 10 11 2.93 12 3.20 13 3.47
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 11 11 2.93 11 2.93 11 2.93
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 10 10 2.67 10 2.67 10 2.67
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 10 12 3.20 12 3.20 13 3.47
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 11 11 2.93 11 2.93 11 2.93
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 10 10 2.67 10 2.67 10 2.67
T-11 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 7.031 + 8.438 + 25.312 562.5 ml 11 22 5.87 27 7.20 30 8.00
T-12 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 9.375 + 11.25 + 33.75 750 ml 10 12 3.20 13 3.47 14 3.73
T-13 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 11.719 + 14.062 + 42.187 937.5 ml 11 12 3.20 13 3.47 14 3.73

Table-15: Bio-efficacy of Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC against Leaf folder (After Second application)
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) LD-0 DAA2 LD-5 DAA2 %
Incidence LD-10 DAA2 %
Incidence LD-15 DAA2 %
Incidence
T-1 Untreated - - 133 151 40.27 159 42.40 173 46.13
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 59 61 16.27 63 16.80 67 17.87
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 61 63 16.80 65 17.33 69 18.40
T-4 Bifenthrin 10 % EC 50 500 ml 60 62 16.53 64 17.07 66 17.60
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 13 13 3.47 13 3.47 13 3.47
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 11 11 2.93 11 2.93 11 2.93
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 10 10 2.67 10 2.67 10 2.67
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 13 13 3.47 13 3.47 13 3.47
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 11 11 2.93 11 2.93 11 2.93
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 10 10 2.67 10 2.67 10 2.67
T-11 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 7.031 + 8.438 + 25.312 562.5 ml 30 32 8.53 34 9.07 38 10.13
T-12 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 9.375 + 11.25 + 33.75 750 ml 14 15 4.00 16 4.27 17 4.53
T-13 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 11.719 + 14.062 + 42.187 937.5 ml 14 15 4.00 16 4.27 17 4.53

Table-16: Phytotoxicity of Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC against Stem borer(After First Application).
Treatments
Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) 12 Hours AA1 1 DAA1 3 DAA1 5 DAA1 7 DAA1 10 DAA1
T-1 Untreated - - 0 0 0 0 0 0
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 0 0 0 0 0 0
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 0 0 0 0 0 0
T-4 Bifenthrin 10 % EC 50 500 ml 0 0 0 0 0 0
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 0 0 1 3 3 3
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 0 0 1 4 5 5
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 0 0 1 5 6 6
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 0 0 0 0 0 0
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 0 0 0 0 0 0
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 0 0 0 0 0 0
T-11 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 7.031 + 8.438 + 25.312 562.5 ml 0 0 0 0 0 0
T-12 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 9.375 + 11.25 + 33.75 750 ml 0 0 0 0 0 0
T-13 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 11.719 + 14.062 + 42.187 937.5 ml 0 0 0 0 0 0

Table-17: Phytotoxicity of Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC against Stem borer (After Second Application)
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) 12 Hours AA2 1 DAA2 3 DAA2 5 DAA2 7 DAA2 10 DAA2
T-1 Untreated - - 0 0 0 0 0 0
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 0 0 0 0 0 0
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 0 0 0 0 0 0
T-4 Bifenthrin 10 % EC 50 500 ml 0 0 0 0 0 0
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 0 0 1 3 3 3
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin10 % EC 8.08 + 12.5 + 50 1175 ml 0 0 1 4 5 5
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 0 0 1 5 6 6
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 0 0 0 0 0 0
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 0 0 0 0 0 0
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 0 0 0 0 0 0
T-11 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 7.031 + 8.438 + 25.312 562.5 ml 0 0 0 0 0 0
T-12 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 9.375 + 11.25 + 33.75 750 ml 0 0 0 0 0 0
T-13 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 11.719 + 14.062 + 42.187 937.5 ml 0 0 0 0 0 0

Table-18: Phytotoxicity of Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC against Leaf folder (After First Application)
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) 12 Hours AA1 1 DAA1 3 DAA1 5 DAA1 7 DAA1 10 DAA1
T-1 Untreated - - 0 0 0 0 0 0
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 0 0 0 0 0 0
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 0 0 0 0 0 0
T-4 Bifenthrin 10 % EC 50 500 ml 0 0 0 0 0 0
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 0 0 1 3 3 3
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 0 0 1 4 5 5
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 0 0 1 5 6 6
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 0 0 0 0 0 0
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 0 0 0 0 0 0
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 0 0 0 0 0 0
T-11 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 7.031 + 8.438 + 25.312 562.5 ml 0 0 0 0 0 0
T-12 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 9.375 + 11.25 + 33.75 750 ml 0 0 0 0 0 0
T-13 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 11.719 + 14.062 + 42.187 937.5 ml 0 0 0 0 0 0

Table-19: Phytotoxicity of Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC against Leaf folder(After Second Application)
Treatments Treatment details Dose
g(a.i./ha) Dose (Formulation/ha) 12 Hours AA2 1 DAA2 3 DAA2 5 DAA2 7 DAA2 10 DAA2
T-1 Untreated - - 0 0 0 0 0 0
T-2 Emamectin Benzoate 01.90 % EC 8.08 425 ml 0 0 0 0 0 0
T-3 Lambda-cyhalothrin 5 % EC 12.5 250 ml 0 0 0 0 0 0
T-4 Bifenthrin 10 % EC 50 500 ml 0 0 0 0 0 0
T-5 Emamectin Benzoate 01.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 6.06 + 9.375 + 37.5 881.25 ml 0 0 1 3 3 3
T-6 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 8.08 + 12.5 + 50 1175 ml 0 0 1 4 5 5
T-7 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 10.1 + 15.625 + 62.5 1468.75 ml 0 0 1 5 6 6
T-8 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 3.03 + 4.68 + 18.75 440.62 ml 0 0 0 0 0 0
T-9 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 4.04 + 6.25 + 25 587.5 ml 0 0 0 0 0 0
T-10 Emamectin Benzoate 1.90% EC + Lambda-cyhalothrin 5 % EC +Bifenthrin 10 % EC 5.05 + 7.81 + 31.25 734.38 ml 0 0 0 0 0 0
T-11 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 7.031 + 8.438 + 25.312 562.5 ml 0 0 0 0 0 0
T-12 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 9.375 + 11.25 + 33.75 750 ml 0 0 0 0 0 0
T-13 Emamectin benzoate 1.0 % + Lambda-cyhalothrin 2.0 % + Bifenthrin 3.0 % EC 11.719 + 14.062 + 42.187 937.5 ml 0 0 0 0 0 0

Result and conclusion:
As seen in Table 3 to 6, treatment number 12 and 13 (dosages of 750 ml and 937.5 ml per hectare or 300mland 375 ml/acre) indicated that maximum percent control of Stem borer and Leaf folder in rice crop over 1-15 DAA.
Also, in Table 8 to 11, treatment number11 and 13 (dosages of 562.5 ml, 750 ml and 937.5 ml per hectare or 225 ml, 300 ml and 375 ml/acre) has no phytotoxicity symptoms.
, Claims:I/We Claim:

1. An insecticidal composition comprising:
a) Emamectin benzoate in an amount ranging from 0.1 - 20% w/w,
b) Lambda-cyhalothrin an amount ranging from 0.5 - 25% w/w; and
c) Bifenthrin in an amount ranging from 0.5 to 30%w/w.

2. The insecticidal composition as claimed in claim 1, wherein Emamectin benzoate is present in an amount of 1.5% w/w, Lambda cyhalothrin is present in an amount of 1.25% w/w and Bifenthrin is present in an amount of 4.5 % w/w.

3. The insecticidal composition as claimed in claim 1, comprising at least one agriculturally acceptable excipient.

4. The insecticidal composition as claimed in claim 1, wherein said insecticidal composition is formulated in a form selected from the group consisting of water-soluble concentrates (SL), emulsifiable concentrates (EC), emulsions (EW), micro-emulsions (ME), Suspension concentrates (SC), oil-based suspension concentrates (OD), flowable suspensions (FS), water-dispersible granules (WG), water-soluble granules (SG), wettable powders (WP), water soluble powders (SP), dry flowables (DF), granules (GR), encapsulated granules (CG), fine granules (FG), macrogranules (GG), aqueous Suspo-emulsions (SE), capsule suspensions (CS), and microgranules (MG).

5. The insecticidal composition as claimed in claim 4, wherein said insecticidal composition is formulated in the form of a Emulsion concentrate (EC).

6. The insecticidal composition as claimed in claim 5, comprising at least a surfactant; at least a solvent; at least co solvent.

7. The insecticidal composition as claimed in claim 6, wherein said surfactants are selected form fatty acid esters, fatty alcohols, fatty acid amides or fatty amines with ethylene and/or propylene oxide, alkyl- , alkenyl or polyaryl-substituted phenols with ethylene and/or propylene oxide, fatty esters of polyhydric alcohol esters, Blend (Non-ionic- Alcohol ethoxylates) and Ionic- (Alkylarylsulphonates) .

8. The insecticidal composition as claimed in claim 6, wherein said co-solvent is selected from dimethyl formamide, cyclohexanone, C-9, isophoron and N-methylpyrrolidone.

9. A method for controlling insects in rice, said method comprising treating rice crop with an insecticidal composition as claimed in claim 1.

10. A process for preparing an insecticidal composition as claimed in claim 1 as an Emulsion concentrate formulation, said process comprising:
(a) Adding and mixing of NMP and Emamectin benzoate and mixed it for half an hour to obtain pre-mix.
(b) Adding cyclohexane into the premix and stir @ 1500RPM for another half an hour.
(c) Adding lambda cyhalothrin and Bifenthrin one by one to the reaction mixture followed by adding C-9 solvent under continuous stirring it for 1 hour.
(d) Adding blend of emulsifier in appropriate quantity and mix it for half an hour to get clear solution.