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Kit For Isothermal Dna Amplification Starting From An Rna Template
Abstract: A method of amplifying RNA template is provided. The method comprises reverse transcribing a ribonucleic acid (RNA) template to form a cDNA using a first reaction mixture comprising RNA template at least one primer capable of hybridizing to the RNA template a reverse transcriptase and deoxynucleoside triphosphates (dNTPs); and amplifying the cDNA to form an amplified product using a second reaction mixture comprising at least one strand displacement DNA polymerase at least one inosine containing primer and a nuclease that is capable of nicking DNA 3 to an inosine residue of the primer. The method is accomplished under an isothermal condition without denaturing the cDNA template. A method of quantifying RNA template in a sample and a method of detecting RNA template in a sample are also provided. Kits for amplifying deoxynucleic acid (DNA) from ribonucleic acid (RNA) template are also provided.
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Notices, Deadlines & Correspondence
Notices, Deadlines & Correspondence
Patent Information
Application #
Filing Date
29 December 2014
Publication Number
36/2016
Publication Type
INA
Invention Field
CHEMICAL
Status
Email
Parent Application
Patent Number
Legal Status
Grant Date
2021-06-17
Renewal Date
Applicants
GENERAL ELECTRIC COMPANY
1 River Road
Schenectady New York 12345
Inventors
1. NELSON John Richard
One Research Circle
Niskayuna New York 12309
2. DUTHIE Robert Scott
One Research Circle
Niskayuna New York 12309
3. GROSSMANN Gregory Andrew
One Research Circle
Niskayuna New York 12309
4. HELLER Ryan Charles
One Research Circle
Niskayuna New York 12309
Specification
Documents
Application Documents
| # | Name | Date |
|---|---|---|
| 1 | 11179-DELNP-2014-RELEVANT DOCUMENTS [01-08-2023(online)].pdf | 2023-08-01 |
| 1 | Other relevant documents.pdf | 2014-12-30 |
| 2 | GPA.pdf | 2014-12-30 |
| 2 | 11179-DELNP-2014-US(14)-HearingNotice-(HearingDate-30-09-2020).pdf | 2021-10-17 |
| 3 | Form PCT-IB-304.pdf | 2014-12-30 |
| 3 | 11179-DELNP-2014-IntimationOfGrant17-06-2021.pdf | 2021-06-17 |
| 4 | Form 5.pdf | 2014-12-30 |
| 4 | 11179-DELNP-2014-PatentCertificate17-06-2021.pdf | 2021-06-17 |
| 5 | Form 3.pdf | 2014-12-30 |
| 5 | 11179-DELNP-2014-8(i)-Substitution-Change Of Applicant - Form 6 [16-10-2020(online)]-1.pdf | 2020-10-16 |
| 6 | Form 2 + Specification.pdf | 2014-12-30 |
| 6 | 11179-DELNP-2014-8(i)-Substitution-Change Of Applicant - Form 6 [16-10-2020(online)].pdf | 2020-10-16 |
| 7 | 11179-delnp-2014-Correspondence Others-(13-01-2015).pdf | 2015-01-13 |
| 7 | 11179-DELNP-2014-ASSIGNMENT DOCUMENTS [16-10-2020(online)]-1.pdf | 2020-10-16 |
| 8 | 11179-DELNP-2014.pdf | 2015-01-16 |
| 8 | 11179-DELNP-2014-ASSIGNMENT DOCUMENTS [16-10-2020(online)].pdf | 2020-10-16 |
| 9 | 11179-DELNP-2014-PA [16-10-2020(online)]-1.pdf | 2020-10-16 |
| 9 | 11179-delnp-2014-Correspondence Others-(22-01-2015).pdf | 2015-01-22 |
| 10 | 11179-delnp-2014-Assignment-(22-01-2015).pdf | 2015-01-22 |
| 10 | 11179-DELNP-2014-PA [16-10-2020(online)].pdf | 2020-10-16 |
| 11 | 11179-DELNP-2014-PETITION UNDER RULE 137 [14-10-2020(online)].pdf | 2020-10-14 |
| 11 | Marked-up copy.pdf | 2015-03-12 |
| 12 | 11179-DELNP-2014-Written submissions and relevant documents [14-10-2020(online)].pdf | 2020-10-14 |
| 12 | Form 13.pdf | 2015-03-12 |
| 13 | 11179-DELNP-2014-FORM-26 [29-09-2020(online)].pdf | 2020-09-29 |
| 13 | Covering letter.pdf | 2015-03-12 |
| 14 | 11179-DELNP-2014-Correspondence to notify the Controller [26-09-2020(online)].pdf | 2020-09-26 |
| 14 | Amended claims.pdf | 2015-03-12 |
| 15 | 11179-DELNP-2014-ABSTRACT [06-02-2020(online)].pdf | 2020-02-06 |
| 15 | 11179-delnp-2014-Form-3-(21-10-2015).pdf | 2015-10-21 |
| 16 | 11179-DELNP-2014-CLAIMS [06-02-2020(online)].pdf | 2020-02-06 |
| 16 | 11179-delnp-2014-Correspondence Others-(21-10-2015).pdf | 2015-10-21 |
| 17 | 11179-DELNP-2014-COMPLETE SPECIFICATION [06-02-2020(online)].pdf | 2020-02-06 |
| 17 | Form 18 [01-06-2016(online)].pdf | 2016-06-01 |
| 18 | 11179-DELNP-2014-DRAWING [06-02-2020(online)].pdf | 2020-02-06 |
| 18 | Form 3 [23-08-2016(online)].pdf | 2016-08-23 |
| 19 | 11179-DELNP-2014-RELEVANT DOCUMENTS [22-08-2017(online)].pdf | 2017-08-22 |
| 19 | 11179-DELNP-2014-FER_SER_REPLY [06-02-2020(online)].pdf | 2020-02-06 |
| 20 | 11179-DELNP-2014-Changing Name-Nationality-Address For Service [22-08-2017(online)].pdf | 2017-08-22 |
| 20 | 11179-DELNP-2014-OTHERS [06-02-2020(online)].pdf | 2020-02-06 |
| 21 | 11179-DELNP-2014-AMENDED DOCUMENTS [22-08-2017(online)].pdf | 2017-08-22 |
| 21 | 11179-DELNP-2014-SEQUENCE LISTING [06-02-2020(online)].txt | 2020-02-06 |
| 22 | 11179-DELNP-2014-Complete Specification.pdf | 2019-11-21 |
| 22 | 11179-DELNP-2014-FER.pdf | 2019-11-21 |
| 23 | 11179-DELNP-2014-Complete Specification.pdf | 2019-11-21 |
| 23 | 11179-DELNP-2014-FER.pdf | 2019-11-21 |
| 24 | 11179-DELNP-2014-AMENDED DOCUMENTS [22-08-2017(online)].pdf | 2017-08-22 |
| 24 | 11179-DELNP-2014-SEQUENCE LISTING [06-02-2020(online)].txt | 2020-02-06 |
| 25 | 11179-DELNP-2014-OTHERS [06-02-2020(online)].pdf | 2020-02-06 |
| 25 | 11179-DELNP-2014-Changing Name-Nationality-Address For Service [22-08-2017(online)].pdf | 2017-08-22 |
| 26 | 11179-DELNP-2014-FER_SER_REPLY [06-02-2020(online)].pdf | 2020-02-06 |
| 26 | 11179-DELNP-2014-RELEVANT DOCUMENTS [22-08-2017(online)].pdf | 2017-08-22 |
| 27 | 11179-DELNP-2014-DRAWING [06-02-2020(online)].pdf | 2020-02-06 |
| 27 | Form 3 [23-08-2016(online)].pdf | 2016-08-23 |
| 28 | 11179-DELNP-2014-COMPLETE SPECIFICATION [06-02-2020(online)].pdf | 2020-02-06 |
| 28 | Form 18 [01-06-2016(online)].pdf | 2016-06-01 |
| 29 | 11179-DELNP-2014-CLAIMS [06-02-2020(online)].pdf | 2020-02-06 |
| 29 | 11179-delnp-2014-Correspondence Others-(21-10-2015).pdf | 2015-10-21 |
| 30 | 11179-DELNP-2014-ABSTRACT [06-02-2020(online)].pdf | 2020-02-06 |
| 30 | 11179-delnp-2014-Form-3-(21-10-2015).pdf | 2015-10-21 |
| 31 | 11179-DELNP-2014-Correspondence to notify the Controller [26-09-2020(online)].pdf | 2020-09-26 |
| 31 | Amended claims.pdf | 2015-03-12 |
| 32 | 11179-DELNP-2014-FORM-26 [29-09-2020(online)].pdf | 2020-09-29 |
| 32 | Covering letter.pdf | 2015-03-12 |
| 33 | 11179-DELNP-2014-Written submissions and relevant documents [14-10-2020(online)].pdf | 2020-10-14 |
| 33 | Form 13.pdf | 2015-03-12 |
| 34 | 11179-DELNP-2014-PETITION UNDER RULE 137 [14-10-2020(online)].pdf | 2020-10-14 |
| 34 | Marked-up copy.pdf | 2015-03-12 |
| 35 | 11179-delnp-2014-Assignment-(22-01-2015).pdf | 2015-01-22 |
| 35 | 11179-DELNP-2014-PA [16-10-2020(online)].pdf | 2020-10-16 |
| 36 | 11179-delnp-2014-Correspondence Others-(22-01-2015).pdf | 2015-01-22 |
| 36 | 11179-DELNP-2014-PA [16-10-2020(online)]-1.pdf | 2020-10-16 |
| 37 | 11179-DELNP-2014.pdf | 2015-01-16 |
| 37 | 11179-DELNP-2014-ASSIGNMENT DOCUMENTS [16-10-2020(online)].pdf | 2020-10-16 |
| 38 | 11179-delnp-2014-Correspondence Others-(13-01-2015).pdf | 2015-01-13 |
| 38 | 11179-DELNP-2014-ASSIGNMENT DOCUMENTS [16-10-2020(online)]-1.pdf | 2020-10-16 |
| 39 | Form 2 + Specification.pdf | 2014-12-30 |
| 39 | 11179-DELNP-2014-8(i)-Substitution-Change Of Applicant - Form 6 [16-10-2020(online)].pdf | 2020-10-16 |
| 40 | Form 3.pdf | 2014-12-30 |
| 40 | 11179-DELNP-2014-8(i)-Substitution-Change Of Applicant - Form 6 [16-10-2020(online)]-1.pdf | 2020-10-16 |
| 41 | Form 5.pdf | 2014-12-30 |
| 41 | 11179-DELNP-2014-PatentCertificate17-06-2021.pdf | 2021-06-17 |
| 42 | Form PCT-IB-304.pdf | 2014-12-30 |
| 42 | 11179-DELNP-2014-IntimationOfGrant17-06-2021.pdf | 2021-06-17 |
| 43 | 11179-DELNP-2014-US(14)-HearingNotice-(HearingDate-30-09-2020).pdf | 2021-10-17 |
| 43 | GPA.pdf | 2014-12-30 |
| 44 | 11179-DELNP-2014-RELEVANT DOCUMENTS [01-08-2023(online)].pdf | 2023-08-01 |
| 44 | Other relevant documents.pdf | 2014-12-30 |
Search Strategy
| 1 | searchstrategy_18-11-2019.pdf |