DESC:KIT TO DETECT MILK ADULTERATION ,CLAIMS:1. A rapid milk-adulteration detection kit comprising
i. a substrate; and
ii. one or more test strips attached to the substrate,
wherein the one or more test strips is independently treated with reagents for detection of one or more milk adulterants selected from a group consisting of glucose, hydrogen peroxide, cane sugar, malt dextrin, urea, ammonia, sulfates, neutralizers, formalin, detergent, starch, and nitrate ions.
2. A rapid milk-adulteration detection kit comprising one or more test strips, the test strips may optionally attached to a substrate, wherein
i. at least one test strip is capable of converting a first adulterant to oxygen;
ii. at least one test strip is capable of converting a second adulterant to ammonia gas;
iii. at least one test strip is capable of detecting adulterants selected from urea, sulfate ions, alkalinity, formalin, detergent, starch, or nitrate ions;
3. The rapid milk-adulteration detection kit as claimed in claim 2, wherein the first adulterant is glucose, hydrogen peroxide, cane sugar or malt dextrin.
4. The rapid milk-adulteration detection kit as claimed in claim 2, comprising of:
i. a test strip coated with a mixture of reagents citric acid, citric hydroxide, glucoseoxidase, hemoglobin and o-toridine;
ii. a test strip coated with a mixture of hemoglobin and o-toridine dissolved in phosphate buffer;
iii. a test strip coated with a mixture of reagents citric acid, citric hydroxide, glucoseoxidase, hemoglobin, o-toridine and glucoamylase;
iv. a test strip coated with a mixture of reagents citric acid, citric hydroxide, glucoseoxidase, hemoglobin, o-toridine and alphaglucosidase;
v. a test strip coated with a mixture of urease and bromothymol blue dissolved in buffer;
vi. a test strip coated with dimethylsulfide dissolved in water;
vii. a test strip coated with p-rosalic acid dissolved in water;
viii. a test strip and acidified Schiff’s reagent;
ix. a test strip, crystal violet reagent dissolved in water, wherein the crystal violet reagent solution is optionally acidified ;
x. a test strip coated with a mixture of iodine and potassium iodide dissolved in water;
xi. a three layered sandwiched test strip, having first layer coated with alkaline solution; third layer coated with bromothymol solution and placing intermediate layer a blank LH-004 gas filter paper; and
xii. a zinc powder test strip, GR reagent and 1M-HCl.
5. A method of detection of adulteration in milk comprising contacting diluted milk sample with a test strip of the kit as claimed in claim 2 or 4, drying the test strip and observing change in color of the test strip.
6. The method as claimed in claim 5, wherein color change from off-white to blue color of test strip of claim 4(i) indicates presence of glucose as adulterant;
7. The method as claimed in claim 5, wherein color change of the test strip of claim 4(ii) from white /off-white to navy blue color indicates presence of hydrogen peroxide as adulterant.
8. The method as claimed in claim 5, wherein color change of the test strip of claim 4 (iii) from off white to navy blue indicates presence of cane sugar as adulterant;
9. The method as claimed in claim 5, wherein color change of the test strip of claim 4 (iv) from off white to navy blue indicates the presence of malt dextrin as adulterant.
10. The method as claimed in claim 5, color change of the test strip of claim 4 (v) from orange to navy blue indicates presence of urea.
11. The method as claimed in claim 5, color change of test strip of claim 4 (vi) from dark blue to pink indicates presence of sulfate.
12. The method as claimed in claim 5, color change of test strip of claim 4(vii) from orange to pink indicates presence of neutralizer.
13. The method as claimed in claim 5, color change of the sandwiched test strip of claim 4(xi) to navy blue indicates presence of ammonia.
14. The method as claimed in claim 5, wherein the test strip of claim 4(viii) is immersed in a milk solution, adding few drops of acidified Schiff reagent to the test strip and observing color change of test strip from white to pink indicates presence of formalin.
15. The method as claimed in claim 5, wherein a few drops of acidified crystal violet reagent and 1 to 2 ml of milk sample is mixed, the milk-reagent solution is placed on a test strip, wiping the excess milk-reagent solution and observing color change of the test strip from white to purple indicates presence of detergents.
16. The method as claimed in claim 5, color change of test strip of claim 4(x) from white to blue indicates presence of starch.
17. The method as claimed in claim 5, wherein the zinc strip is contacted with acidified milk sample and few drops of GR reagent is added to the zinc strip coated with milk sample and observing color change from white to red purple indicates the presence of nitrate.
18. A rapid milk-adulteration detection kit for detecting presence of glucose as adulterant in milk comprising a test strip coated with a mixture of citric acid, citric hydroxide, glucoseoxidase, hemoglobin and O-toridine, wherein the test strip’s color after contacting with subject milk changes from off-white to blue color.
19. A rapid milk-adulteration detection kit for detecting presence of hydrogen-peroxide as adulterant in milk comprising a test strip coated with a mixture of hemoglobin and O-toridine dissolved in phosphate buffer, wherein the test strip’s color after contacting with subject milk, changes from off-white to blue color.
20. A rapid milk-adulteration detection kit for detecting presence of cane sugar as adulterant comprising a test strip coated with a mixture of citric acid, citric hydroxide, glucoseoxidase, glucoamylase, hemoglobin and O-toridine, wherein the test strip’s color after contacting with subject milk, changes from off-white to navy blue color.
21. A rapid milk-adulteration detection kit for detecting presence of malt-dextrin as adulterant comprising a test strip coated with a mixture of citric acid, citric hydroxide, glucoseoxidase, glucoamylase, alfa-glucodidase, hemoglobin and O-toridine, wherein the test strip’s color after contacting with subject milk, changes from off-white to blue color.
22. A rapid milk-adulteration detection kit for detecting presence of urea as a adulterant comprising a test strip coated with a mixture of urease and bromothymol blue dissolved in buffer, wherein the test strip’s color after contacting with subject milk, changes from orange to navy blue.
23. A rapid milk-adulteration detection kit for detecting presence of sulphates as a adulterant comprising a test strip coated with dimethylsulfide dissolved in water, wherein the test strip’s color after contacting with subject milk, changes from dark blue to pink.
24. A rapid milk-adulteration detection kit for detecting presence of neutralizer as a adulterant comprising a test strip coated with p-rosalic acid dissolved in water, wherein the test strip’s color after contacting with subject milk, changes from orange to pink.
25. A rapid milk-adulteration detection kit for detecting presence of formalin as an adulterant comprising acidified Schiff’s reagent and a test strip dipped in subject milk, wherein adding the Schiff’s reagent to the test strip, the color changes from white to pink.
26. A rapid milk-adulteration detection kit for detecting presence of detergent as a adulterant comprising a test strip, crystal violet reagent dissolved in water, the crystal violet reagent solution is optionally acidified, wherein by placing 1 to 2 mil of milk sample mixed with few drops of acidified crystal violet reagent, the test strip develops a color change from white to purple.
27. A rapid milk-adulteration detection kit for detecting presence of starch as a adulterant comprising a test strip coated with a mixture of iodine and potassium iodide dissolved in water, wherein the test strip develops a color change from white to blue.
28. A rapid milk-adulteration detection kit for detecting presence of nitrate as a adulterant comprising a zinc powder strip, GR reagent and 1M-HCl, wherein the zinc strip is contacted with acidified milk sample and a few drops of GR reagent is added to the zinc strip contacted with milk sample and observing color change from white to red purple indicates the presence of nitrate.

29. A rapid milk-adulteration detection kit for detecting presence of ammonia as a adulterant comprising a three layered sandwiched test strip, having first layer coated with alkaline solution; third layer coated with bromothymol solution and placing intermediate layer a blank LH-004 gas filter paper, wherein the test strip’s color change to blue indicates the presence of ammonia.