FIELD OF THE INVENTION:
The present invention relates to a liquid hand wash composition for cleaning means. More particularly, the present invention relates to antimicrobial composition of liquid hand wash comprising of surfactant, cosurfactant, antimicrobial agent, thickener, colorant, binder, water. The present invention further relates to method for the preparation of liquid hand wash for cleaning means.
BACKGROUND AND PRIOR ART OF THE INVENTION:
Conventional soaps used for hand washing do not indicate whether the soap has been used for an appropriate amount of time for the process to be effective. Properly washing your hands is one of the best ways to prevent infection and the spread of diseases. Doctors, nurses and other people who work in medical settings have to wash their hands frequently to avoid spreading infection between patients or to themselves. Those who prepare food must keep clean hands so they do not put germs into the food they are making. Also, childcare workers must wash their hands often so they do not make children sick. In addition to professionals whose hygiene is regulated, individuals should be conscious of the germs on their hands. Turning doorknobs, handling money and coughing may transfer bacteria and viruses to one's hands, which can then be spread to other people's hands or to your mouth and eyes and cause infection. Approaches ensuring effective hand cleansing include antibacterial hand rubs. One drawback with such hand rubs is that such rubs may dry hands more than washing with soap. Another approach known involves applying a dye that glows under a black light to hands prior to washing hands, washing hands, and then analyzing the effectiveness of the hand washing process by examining the hands under

a black light. In addition, some hospitals use electronic devices that alert an employee if the employee's hands were not effectively washed. Such approaches may be expensive and fail to achieve widespread use. The present invention has many advantages as it is an easy, convenient, and inexpensive approach to facilitate effective hand washing.
There is continuing need for very mild skin cleansers with effective antibacterial liquid hand wash. The addition of an antibacterial effective amount of an antibacterial agent to a very mild liquid composition can potentially bring about undesirable effects such as skin dryness and skin irritation. The undesirable effects can manifest themselves after repeated washings as well as after infrequent use. These undesirable characteristics have been addressed in a liquid cleansing composition which is ultra mild to the skin while providing effective antibacterial activity. Additional agents providing additional lather activity while essentially retaining the ultra mildness can also be employed in the composition.
Another important concern for a wash formulation is the aesthetics of the product. For example, the public has come to associated foaming ability with cleaning ability, and, as a result, consumers are less likely to purchase a wash formulation that does not foam while washing. This consumer perception drives those in the market to formulate washing products which produce copious amounts of foam. As mentioned above, irritancy is a concern. The color and odor of a wash formulation is also important. When soaps are used in conjunction with antimicrobial agents, they do not present the best of each of these desired properties. Although the presence of the soap allows for a reduction in the amount of antimicrobial agent while maintaining a relatively high log kill, antimicrobial soap products tend to irritate the skin. Thus, skin conditioning agents need added to produce an aesthetically pleasing hand wash.

Currently, various method and composition have been disclosed for the preparation of liquid hand wash for cleaning means and it has reported some drawbacks as optimum antibacterial activity with undesirable smell, irritancy to skin and reported clogging of tip of dispenser by solidifying on standing etc.
U.S. Patent application no. US 6,117,828 A discloses a hand wash compositions wherein, composition of hand wash or personal wash shampoo comprising surfactant(s), cationic wheat protein and an antibacterial agent. The cationic wheat protein provides enhanced skin feel relative to other proteins.
U.S. Patent application no. US 5,681,802 relate to a liquid skin cleansing composition and disclosing composition containing a mild surfactant system, a pH buffer, and an antibacterial agent.
As evident from the prior art, it becomes challenging to provide compositions which have maximum antimicrobial properties, mildness, smell, lather, performance, skin feel. The present invention relates to composition of liquid hand wash which does not clog the tip of a liquid dispenser by solidifying on standing. Thus, the present invention holds an advantage of simple, economic efficient and safer composition of liquid hand wash for cleaning means. The present invention also provides simple, applicable and cost effective method for preparation of liquid hand wash for cleaning means.
The discussion of documents, acts, materials, devices, articles and the like is included in this specification solely for the purpose of providing a context for the present invention. It is not

suggested or represented that any or all of these matters formed part of the prior art base or were common general knowledge in the field relevant to the present invention as it existed before the priority date of each claim of this application.
OBJECTIVES OF THE INVENTION
• A primary objective of the present invention is to provide liquid hand wash composition for cleaning means.
• Another objective of the present invention is to provide antimicrobial composition of liquid hand wash for cleaning means.
• Another objective of the present invention is to provide simple and economically efficient composition for the preparation of liquid hand wash.
• Another objective of the present invention is to provide method for the preparation of liquid hand wash for cleaning means.
• Another objective of the present invention is to provide liquid hand wash with safer and low cost composition for cleaning means.
• A further objective of the invention is to provide a stable liquid composition that has a pleasant smell and does not leave a sustained medicinal odor on the hands or skin after use.
• Another objective of the present invention is to provide a composition that is mild and does not irritate or dry the skin after frequent use.

SUMMARY OF INVENTION
The present invention relates to a liquid hand wash composition for cleaning means.
According to the present invention there is provided a composition of liquid hand wash containing surfactant, antimicrobial agent, ethylene glycol monostearate, cocamidopropyl betaine, methyl paraben, sodium chloride, citric acid, water, colorant, perfume, preservatives.
Accordingly, the percentage of each ingredient in preparation of liquid hand wash is 1) atleast 15-30% w/w of Surfactant 2) atleast 0-1.5% w/w Ethylene glycol monostearate 3) atleast 0-4 %w/w Cocamidopropyl betaine 4) atleast 0.50 - 1% w/w Antimicrobial agent 5) atleast 0.05% w/w Methyl paraben 6) atleast 1-1.10% w/w Sodium chloride 7) atleast 0.20-0.50 % w/w Perfume 8) atleast 0.02-0.05 % w/w Colorant 9) atleast 0.01-0.02 % w/w Citric acid and atleast 70-75% w/w Demineralized water
According to the one more embodiment of the present invention, it provides the process for preparation of said composition comprising step of taking Sodium lauryl ethyl sulphate and heating of said ingredient at 75° C.then add Ethyl glycol monostearate while heating and mix above ingredients till it dissolved completely. Then addition of demineralised water in above said mixture and mixing at speed of 30rpm and further addition of colorant in to above said mixture and mixes it for 2 minute. Again mixing of above said mixture of Sodium lauryl sulphate and ethyl glycol monostearate for 2 minutes, then add cocamido propyl betaine , methyl paraben and citric acid in above said mixture and mix it for 3 minutes. Further addition of triclosan in the mixture and mix it for 2 minutes. Finally addition of sodium chloride and perfume in to resultant reaction mixture and mixing carried out for 10 minutes. Mixing all the ingredients thoroughly till

mixture get homogenize. Finally resultant product is obtained and analysis carried out by various test.
STATEMENT OF THE INVENTION:
The present invention relates to a composition of liquid hand wash for cleaning means. More particularly, the present invention relates to liquid hand wash composing of surfactant, antimicrobial agent, ethylene glycol monostearate, cocamidopropyl betaine, methyl paraben, sodium chloride, water, colorant, perfume, preservatives. The present invention further relates to method for preparation of liquid hand wash for cleaning means.
The present invention relates to composition of liquid hand wash comprising 1) atleast 15-30% w/w of Surfactant 2) atleast 0-1.5% w/w Ethylene glycol monostearate 3) atleast 0-4 %w/w Cocamidopropyl betaine 4) atleast 0.50 - 1% w/w Antimicrobial agent 5) atleast 0.05% w/w Methyl paraben 6) atleast 1-1.10% w/w Sodium chloride 7) atleast 0.20-0.50 % w/w Perfume 8) atleast 0.02-0.05 % w/w Colorant 9) atleast 0.01 - 0.02% w/w Citric acid and atleast 70-75% w/w Demineralized water.
The invention provides a process for preparation of said composition comprising step of taking Sodium lauryl ethyl sulphate and heating of said ingredient at 750 C.then add Ethyl glycol monostearate while heating and mix above ingredients till it dissolved completely. Then addition of demineralised water in above said mixture and mixing at speed of 30rpm and further addition of colorant in to above said mixture and mixes it for 2 minute. Again mixing of above said mixture of Sodium lauryl sulphate and ethyl glycol monostearate for 2 minutes, then add cocamido propyl betaine , methyl paraben and citric acid in above said mixture and mix it for 3 minutes. Further addition of triclosan in the mixture and mix it for 2 minutes. Finally addition of

sodium chloride and perfume in to resultant reaction mixture and mixing carried out for 10 minutes. Mixing all the ingredients thoroughly till mixture get homogenize. Finally resultant product is obtained and analysis carried out by various test.
DESCRIPTION OF THE INVENTION:
While the making and using of various embodiments of the present invention are discussed in detail below, it should be appreciated that the present invention provides many applicable inventive concepts that can be embodied in a wide variety of specific contexts. The specific embodiments discussed herein are merely illustrative of specific ways to make and use the invention and do not delimit the scope of the invention.
To facilitate the understanding of this invention, a number of terms are defined below. Terms defined herein have meanings as commonly understood by a person of ordinary skill in the areas relevant to the present invention. Terms such as "a", "an" and "the" are not intended to refer to only a singular entity, but include the general class of which a specific example may be used for illustration. The terminology herein is used to describe specific embodiments of the invention, but their usage does not delimit the invention, except as outlined in the claims.
The present invention relates to a composition of liquid hand wash for cleaning means. More particularly, the present invention relates to liquid hand wash composing of surfactant, antimicrobial agent, ethylene glycol monostearate, cocamidopropyl betaine, methyl paraben, sodium chloride, water, colorant, perfume, preservatives. The present invention further relates to method for preparation of liquid hand wash for cleaning means.

The main embodiment of the present invention relates to composition of liquid hand wash comprising I) atleast 15-30% w/w of Surfactant 2) atleast 0-1.5% w/w Ethylene glycol monostearate 3) atleast 0-4 %w/w Cocamidopropyl betaine 4) atleast 0.50 - 1% w/w Antimicrobial agent 5) atleast 0.05% w/w Methyl paraben 6) atleast 1-1.10% w/w Sodium chloride 7) atleast 0.20-0.50 % w/w Perfume 8) atleast 0.02-0.05 % w/w Colorant 9) atleast 0.01 - 0.02% w/w>Citric acid and atleast 70-75% w/w Demineralized water.
Another embodiment of the present invention is relates to safe, stable and low cost liquid hand wash containing antimicrobial composition.
According to main embodiment, surfactant system may include at least one surfactant selected from the group consisting of an anionic surfactant, a cationic surfactant, a nonionic surfactant, an amphoteric surfactant, and any combination thereof.
According to main embodiment, thickener used is selected from the group consisting of inorganic salt thickeners such as sodium chloride, potassium chloride, magnesium chloride, potassium sulfate, and sodium sulfate.
According to main embodiment, antimicrobial agent is selected from the group consisting of bisguanidine (e.g., chlorhexidine digluconate), diphenyl compounds, benzyl alcohols, trihalocarbanilides, quaternary ammonium compounds, ethoxylated phenols, and phenolic compounds, such as halo-substituted phenolic compounds, like p-chloro-m-xylenol (known as "pcmx") arid 2,4,4'-trichloro-2' hydroxy-diphenylether (known as "triclosan").
According to main embodiment, colorant used is selected from group consisting of ponaceau 4 R, etc.

According to main embodiment, perfume used is selected from group consisting of perfume peach etc.
According to main embodiment, binder used is selected from group consisting of methyl paraben etc.
Another essential embodiment of the present invention is to provide method for the preparation of liquid hand wash for cleaning means. A method for preparation of liquid hand wash comprises steps of:
a. Taking Sodium lauryl ethyl sulphate and heating of said ingredient at 75° C.
b. Addition of Ethyl glycol monostearate while heating
c. Mixing above ingredients till it dissolved completely.
d. Addition of demineralised water in above said mixture and mixing at speed of 30rpm.
e. Further addition of colorant in to above said mixture and mixes it for 2 minute.
f. Mixing of above said mixture of Sodium lauryl sulphate and ethyl glycol monostearate
for 2 minutes.
g. Addition of cocamido propyl betaine ,methyl paraben and citric acid in above said
mixture and mix it for 3 minutes.
h. Further addition of triclosan in the mixture and mix it for 2 minutes.
i. Finally addition of sodium chloride and perfume in to resultant reaction mixture and mixing carried out for 10 minutes.
j. Mixing all the ingredients thoroughly till mixture get homogenize.
k. Finally resultant product is obtained and analysis carried out by various test. The final product of liquid hand wash for cleaning means is stored, packed and ready to market place.

The present invention is further described with the help of the following examples, which are given by way of illustration all the parts, percent's and ratios are by weight unless otherwise indicated and therefore should not be construed to limit the scope of the invention in any manner. EXAMPLES:
The composition has been described in detail giving the formula of the ingredients along with the method of preparation.
EXAMPLE 1:
To heat Sodium lauryl ethyl sulphate at 75° C. then added Ethyl glycol monostearate while heating and mix above ingredients till it dissolved completely. Then addition of demineralised water in above said mixture and mixing at speed of 30rpm and further addition of colorant in to above said mixture and mixes it for 2 minute. Again mixing of above said mixture of Sodium lauryl sulphate and ethyl glycol monostearate for 2 minutes. Then added cocamido propyl betaine , methyl paraben and citric acid in to above said mixture and mixed it for 3 minutes. Further added triclosan in the mixture and mixed it for 2 minutes. Finally addition of sodium chloride and perfume in to resultant reaction mixture and mixing carried out for 10 minutes. Mixing all the ingredients thoroughly till mixture get homogenize. Finally resultant product is obtained and analysis carried out by various test.
The various tests is carried out to characterize antimicrobial properties, stability and smoothness of prepared liquid hand wash composition.
The following table No. 1 shows the ingredient in the liquid hand wash composition containing SLES and Triclosan in % by weight.

EXAMPLE 2:
Table No. 1

SrNo. Ingredients Each disc contains (%
w/w)
1 Sodium Lauryl Ethyl Sulphate 20
2 Ethylene Glycol Monostearate 1.5
3 Cocamidopropyl betaine 4
4 Triclosan USP 0.10
5 Citric Acid 0.20
6 Methylparaben 0.15
7 Sodium Chloride 1.10
8 D M Water 72.45
9 Ponaceau 4 R 0.01
10 Perfume peach 0.40

The viscosities of the compositions were 780 and 900 cps, and it is adjusted by the addition of sodium chloride and it is as measured by a Brookfield viscosimeter with a 20 rpm with number 3 spindle. Citric acid (0.20%) was used to adjust the pH to 6.5-7.0. The target specific gravity was 1.022, and the compositions had a clear, peach colour. The pH of composition was 6.4 and the compositions passed the E2 antimicrobial test.
EXAMPLE 3:
The compositions mentioned in Table 1 were formulated and the antimicrobial properties of each were evaluated by an antimicrobial time-kill test method. The time-kill test method measures the in vitro ability of the antimicrobial composition to rapidly reduce a known population of bacteria. A 50% dilution of each composition was brought into contact with a known population of test bacterial for a specified period of time (30 seconds) at a specified temperature. The antimicrobial ingredients were neutralized at the end of the test period and the sample was plated to enumerate the surviving bacteria. The percent reduction from the original population was calculated.
In vitro efficacy of these compositions was measured against of E. coli, S. aureus, E. faecium, and S. marcescens (Group 1). Efficacy was also measured against a mixture of S. aureus (MRSA), P. mirabilis, K. pneumoniae, and S. epidermidis (Group 2). The test was conducted according to the ASTM E 2315 method, "Standard Guide for Assessment of Antimicrobial

Activity Using a Time-Kill Procedure." Contact time was 15 seconds. Results are summarized in table no. 3 Table No. 3

E. coli ATCC 10536

Clean conditions Dirty conditions

80% 50% 25% 80% 50% 25%
Day 14 Ambient >5.35 >5.35 >5.35 - - -
Day 57
Ambient - - >5.3 >5.3 >5.3
16 months >6.70 - - >6.70 - -
Day 52 at 40 C >5.32 >5.32 >5.32 - -
7 months at40C >6.45 >6.45 >6.45 >6.45 >6.45 >6.45
16 months - - >6.70 - -
EXAMPLE 4:
In vitro efficacy of these compositions was measured against of S. aureus, .Efficacy was also measured and the test was conducted according to the ASTM E 2315 method, "Standard Guide

for Assessment of Antimicrobial Activity Using a Time-Kill Procedure." Contact time was 15 seconds. Results are summarized in table no. 4
Table No. 4

S. aureus ATCC 6538

Clean conditions Dirty conditions

80% 50% 25% 80% 50% 25%
Day 14 Ambient >5.06 >5.06 >5.06 - - -
Day 57 Ambient ~ ~ " >5.09 >5.09 >5.09
16 months >6.50 - - >6.50 - -
Day 52 at 40 C >5.19 >5.19 >5.19 - - -
7 months at40C >6.36 >6.36 >6.36 >6.36 >6.36 >6.36
16 months - - - >6.50 - -
EXAMPLE 5:
In vitro efficacy of these compositions was measured against of P. aeru2inosa, .Efficacy was also measured and the test was conducted according to the ASTM E 2315 method, "Standard Guide

for Assessment of Antimicrobial Activity Using a Time-Kill Procedure." Contact time was 15 seconds. Results are summarized in table no. 5
Table No. 5

P. aeru2inosa ATCC 15442

Clean conditions Dirty conditions

80% 50% 25% 80% 50% 25%
Day 14 Ambien >5.32 >5.32 >5.32 - - -
Day 57 Ambien - - - >5.02 >5.02 >5.02
16 months >6.70 - - >6.70 - -
Day 52 at >5.29 >5.29 >5.29 - - -
7 months >6.58 >6.58 >6.58 >6.58 >6.58 >6.58
16 months - - >6.70 -

EXAMPLE 6:
Stability of these compositions was measured at various temperature mentioned in table no. 6 .The stable composition of liquid handwash composition is obtained and results are summarized in table no. 6 Table No. 6

Time 5°C 20°C 40°C
T=0 0.69%
T=2 0.55% 0.52%
T=4 0.54% 0.39%
T=6 0.23% 0.17%
T=8 0.39% 0.24%
T=12 0.27% 0.22%
4 months 0.33% 0.29%
5 months 0.38% 0.21%
6 months 0.36% 0.16%
7 months 0.36% 0.12%
8 months 0.25% 0.14%
9 months 0.35% 0.22%
10 months 0.30% 0.19%
11 months 0.32% 0.15%
12 months 0.43% 0.16% 0.11%
16 months 0.20% 0.07%

We claim;
1. A liquid hand wash composition comprising:
a) atleast 15-30% w/w of Surfactant
b) 0-1.5% w/w Ethylene glycol monostearate
c) 0-4 %w/w Cocamidopropyl betaine
d) 0.50 - I % w/w Antimicrobial agent

e) 0.05% w/w Methyl paraben
f) 1-1.10% w/w Sodium chloride
g) 0.20-0.50 % w/w Perfume
h) 0.02-0.05 % w/w Colorant
i) 0.01 - 0.02% w/w Citric acid and atleast j) 70-75% w/w Demineralized water.
2. The composition claimed in claim 1, wherein at least one anionic surfactant comprises sodium lauryl ether sulfate or sodium lauryl sulfate.
3. The composition claimed in claim 1, wherein at least thickener comprises inorganic salt thickeners selected form group of sodium chloride, potassium chloride, magnesium chloride, potassium sulfate, and sodium sulfate
4. The composition claimed in claim 1, wherein at least one emollient is selected from the group consisting of ethyl wene glycol monostearate, propylene glycol, glycerol, isopropyl myristate and isopropyl palmitate
5. The composition claimed in claim 1, further comprising at least one component selected from the group consisting of stabilizers, humectants, pearlizers, fragrances and dyes.

6. The composition of claim 1, wherein the composition has an effective amount of antimicrobial activity for at least about 15 months.
7. The method for preparation of liquid handwash comprising;

a) Heating Sodium lauryl ethyl sulphate at 75° C
b) Adding Ethyl glycol monostearate followed by addition of water
c) Further addition of colorant and propyl betaine ,methyl paraben and citric acid in above said mixture
d) Further addition of triclosan in the mixture and mix it for 2 minutes.
e) Addition of sodium chloride in to resultant product

8. The method for preparation of liquid handwash claimed in claim 7, wherein mixture is mixed for 3-6 mintute at spped of 30 rpm.
9. The method for preparation of liquid handwash claimed in claim 7, wherein sodium chloride is used and mixed the mixture for 10 minutes to get it homogenize.
10. The liquid handwash composition is used as an antibacterial disinfectant in the industry.