Claims:1. A method for producing albino free double haploid plants of rice through cultures of anthers containing microspores, said method comprising;
(a) obtaining rice boots;
(b) incubating rice boots in moist conditions at a temperature in the range of 8-12°C for 1-12 days;
(c) isolating population of anthers containing microspores from rice boots;
(d) culturing the isolated anthers containing microspores in dark at a temperature in the range of 25 ± 2°C for 4-10 weeks in medium A to obtain calli, wherein said medium A comprises N6 media, 2,4 Dicholorophenoxyaceticacid (2,4 D), 6 benzyl amino purine (BAP), and a carbon source;
(e) transferring the calli to medium B, and incubating under cool white fluorescent light of intensity in the range of 2000-3000 luxwith 16h of light period and 8 h of dark period at a temperature in the range of 25 ± 2°C and relative humidity in the range of 50-60% for 1.5-4 weeks to obtain shoots; wherein said medium B comprises MS minimal salts, a naphthalene acetic acid (NAA), kinetin, BAP, and a carbon source; and
(f) transferring the shoots to medium C for rooting to obtain rice plantlets, wherein medium C comprises MS salts, a napthalene acetic acid (NAA), kinetin, and a carbon source; and
(g) selecting for albino free double haploid rice plantlets. .

2. The method as claimed in claim 1, wherein said method further comprises after step (f) incubating said rice plantlets in conditions favorable for hardening.

3. The method as claimed in claim 1, wherein said rice boot is fromOriyza sativa varietyindica.

4. The method as claimed in claim 1, wherein said medium A comprises N6 media, 2,4D in the concentration range of 1-2.5mg/L, BAP in the concentration range of 0.5 to 2.5 mg/L, and a carbon source.

5. The method as claimed in claim 1, wherein said medium B comprises MS minimal salts, NAA in the concentration range of 0.01-2mg/L, kinetin in the concentration range of 0.01-1.5mg/L, BAP in the concentration range of 0.5-2.5mg/L, a carbon source.

6. The method as claimed in claim 1, wherein said medium C comprises MS salts, NAA in the concentration range of 0.01-2mg/L, kinetin in the concentration range of 0.01-1.5mg/L, a carbon source.

7. The method as claimed in claim 5, wherein said MS minimal salts consists of calcium chloride, ammonium nitrate, potassium nitrate, cobalt chloride, potassium idodide, potassium dihydrophosphate, boric acid, sodium molybedate, magnesium sulphate, manganese sulphate, copper suplate, zinc sulphate, ferrous sulphate, disodium ethylenediaminetetraacectic acid.

8. A medium for producing albino free plants, wherein said medium comprises:
calcium chloride dihydrate; ammonium nitrate;potassium nitrate; cobalt chloride hexahydrate; potassium idodide; potassium dihydrophosphate; boric acid; sodium molybedate dihydrate; magnesium sulphate heptahydrate; manganese sulphate monohydrate; copper sulphate pentahydrate; zinc sulphate heptahydrate; ferrous sulphate heptahydrate, and disodium ethylenediaminetetraacectic acid dihydrate.

9. The medium as claimed in claim 8, wherein calcium chloride dihydrate is present at a concentration of 440mg/L, ammonium nitrate is present at a concentration of 1650mg/L, potassium nitrate is present at a concentration of 1900mg/L, cobalt chloride hexahydrate is present at a concentration of 0.025mg/L, potassium idodide is present at a concentration of 0.83mg/L, potassium dihydrophosphate is present at a concentration of 170mg/L, boric acid is present at a concentration of 6.2mg/L, sodium molybedate dihydrate is present at a concentration of 0.25mg/L, magnesium sulphate heptahydrate is present at a concentration of 370mg/L, manganese sulphate monohydrate is present at a concentration of 16.9mg/L, copper sulphate pentahydrate is present at a concentration of 0.1 mg/L, zinc sulphate hepta hydrate is present at a concentration of 8.6mg/L, ferrous sulphate heptahydrate is present at a concentration of 17.8mg/L, disodium ethylenediaminetetraacectic acid dihydrate is present at a concentration of 37.21mg/L.
, Description:As Attached