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Method For Collecting Nucleic Acid

Abstract: A method of collecting a nucleic acid from a biological sample, comprising the steps of: a) mixing a support comprising a water-soluble neutral polymer adsorbed on a cerium oxide surface thereof with a solution containing a nucleic acid to adsorb the nucleic acid to the support, b) separating the support on which the nucleic acid was adsorbed from the solution mixed in the step a), and c) collecting the nucleic acid by adding an eluent to the support on which the nucleic acid was adsorbed and which is separated in the step b).

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Notices, Deadlines & Correspondence

Patent Information

Application #
Filing Date
10 October 2018
Publication Number
42/2018
Publication Type
INA
Invention Field
BIOTECHNOLOGY
Status
Email
patent@depenning.com
Parent Application

Applicants

TORAY INDUSTRIES, INC.
1-1, Nihonbashi-Muromachi 2-chome, Chuo-ku, Tokyo 103-8666.

Inventors

1. SEKIGUCHI, Shota
c/o Basic Research Center, Toray Industries, Inc., 10-1n Tebiro 6-chome, Kamakura-shi, Kanagawa 248-8555.
2. SAWADA, Shinjiro
c/o Basic Research Center, Toray Industries, Inc., 10-1, Tebiro 6-chomen Kamakura-shin Kanagawa 248-8555.
3. NAKAGAWA, Mai
c/o Basic Research Center, Toray Industries, Inc., 10-ln Tebiro 6-chome, Kamakura-shi, Kanagawa 248-8555.

Specification

1. A method of collecting a nucleic acid from a biological sample, comprising
the steps of:
a) mixing a support comprising a water-soluble neutral polymer adsorbed on a cerium oxide surface thereof with a solution containing a nucleic acid to adsorb the nucleic acid to the support,
b) separating the support on which the nucleic acid was adsorbed from the solution mixed in the step a), and
c) collecting the nucleic acid by adding an eluent to the support on which the nucleic acid was adsorbed and which is separated in the step b).

2. The method of collecting a nucleic acid according to claim 1, wherein the water-soluble neutral polymer is a polymer with a zeta potential of not less than -10 mV and not more than +10 mV in a solution of pH 7.
3. The method of collecting a nucleic acid according to claim 1 or 2, wherein the polymer is polyvinyl alcohol, polyethylene glycol, polyvinylpyrrolidone, poly(2-ethyl-2-oxazoline), polypropylene glycol, polyacrylamide, poly(N-isopropylacrylamide) or hydroxypropylmethylcellulose.
4. The method of collecting a nucleic acid according to any one of claims 1 to 3, wherein the eluent is a buffer solution.
5. The method of collecting a nucleic acid according to any one of claims 1 to 4, wherein the biological sample is blood, urine, saliva, a mucous membrane, sweat, a cultured cell, a culture liquid of cultured cells, a tissue sample, a specimen, a microorganism, a culture liquid of a microorganism or a virus.
6. A support comprising a water-soluble neutral polymer adsorbed on a cerium oxide surface thereof.
7. The support according to claim 6, wherein the water-soluble neutral polymer is a polymer with a zeta potential of not less than -10 mV and not more than +10 mV

in a solution of pH 7.
8. The support according to claim 6 or 7, wherein the water-soluble neutral polymer is polyvinyl alcohol, polyethylene glycol, polyvinylpyrrolidone, poly(2-ethyl-2-oxazoline), polypropylene glycol, polyacrylamide, poly(N-isopropylacrylamide) or hydroxypropylmethylcellulose.
9. A kit for collecting a nucleic acid, comprising the support according to any one of claims 6 to 8 and a buffer solution.

Documents

Application Documents

# Name Date
1 201847038369.pdf 2018-10-10
2 201847038369-TRANSLATIOIN OF PRIOIRTY DOCUMENTS ETC. [10-10-2018(online)].pdf 2018-10-10
3 201847038369-STATEMENT OF UNDERTAKING (FORM 3) [10-10-2018(online)].pdf 2018-10-10
4 201847038369-PROOF OF RIGHT [10-10-2018(online)].pdf 2018-10-10
5 201847038369-PRIORITY DOCUMENTS [10-10-2018(online)].pdf 2018-10-10
6 201847038369-POWER OF AUTHORITY [10-10-2018(online)].pdf 2018-10-10
7 201847038369-FORM 1 [10-10-2018(online)].pdf 2018-10-10
8 201847038369-DECLARATION OF INVENTORSHIP (FORM 5) [10-10-2018(online)].pdf 2018-10-10
9 201847038369-COMPLETE SPECIFICATION [10-10-2018(online)].pdf 2018-10-10
10 201847038369-CLAIMS UNDER RULE 1 (PROVISIO) OF RULE 20 [10-10-2018(online)].pdf 2018-10-10
11 Correspondence by Agent_Form 1_23-10-2018.pdf 2018-10-23
12 201847038369-FORM 3 [12-03-2019(online)].pdf 2019-03-12