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“Method For Culturing Animal Hepatocyte”
Abstract: Disclosed is a technique for culturing a hepatocyte three-dimensionally to form a spheroid readily. Also disclosed is a technique for forming a spheroid in which a transporter MRP2 (which is a transporter involved in the bile duct excretion) can be highly expressed compared with conventional techniques. The conditions under which a hepatocyte can be formed into a spheroid on a nanopillar (NP) sheet readily, specifically the conditions relating to the temperature of type-I collagen to be applied on the NP sheet, are now found. In addition, the conditions under which the expression level of a gene involved in the excretion of the formed spheroid can be increased are also found. Specifically, a spheroid is formed in advance, and a biological matrix is subsequently overlaid on the spheroid.
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Notices, Deadlines & Correspondence
Notices, Deadlines & Correspondence
Patent Information
Application #
Filing Date
06 July 2011
Publication Number
32/2012
Publication Type
INA
Invention Field
BIOTECHNOLOGY
Status
Email
Parent Application
Patent Number
Legal Status
Grant Date
2017-01-13
Renewal Date
Applicants
HITACHI LTD.
6-6 Marunouchi 1-chome Chiyoda-ku Tokyo Japan
Inventors
1. TAKAHASHI Ryosuke
c/o HITACHI LTD. Intellectual Property Group 12th Floor Marunouchi Center Building 6-1 Marunouchi 1-chome Chiyoda-ku Tokyo Japan
2. HISADA Akiko
c/o HITACHI LTD. Intellectual Property Group 12th Floor Marunouchi Center Building 6-1 Marunouchi 1-chome Chiyoda-ku Tokyo Japan
3. SONODA Hiroshi
c/o HITACHI LTD. Intellectual Property Group 12th Floor Marunouchi Center Building 6-1 Marunouchi 1-chome Chiyoda-ku Tokyo Japan
Specification
PLEASE SEE THE SPECIFICATION ATTACHED.
Documents
Application Documents
| # | Name | Date |
|---|---|---|
| 1 | 18-delnp-2011-Form-5-(03-01-2011).pdf | 2011-01-03 |
| 2 | 18-delnp-2011-Form-3-(03-01-2011).pdf | 2011-01-03 |
| 3 | 18-delnp-2011-Form-1-(03-01-2011).pdf | 2011-01-03 |
| 4 | 5218-delnp-2011-GPA-(12-07-2011).pdf | 2011-07-12 |
| 5 | 5218-delnp-2011-Form-5-(12-07-2011).pdf | 2011-07-12 |
| 6 | 5218-delnp-2011-Form-3-(12-07-2011).pdf | 2011-07-12 |
| 7 | 5218-delnp-2011-Form-1-(12-07-2011).pdf | 2011-07-12 |
| 8 | 5218-delnp-2011-Crrospondence others-(12-07-2011).pdf | 2011-07-12 |
| 9 | 5218-DELNP-2011-Form-18-(01-08-2011).pdf | 2011-08-01 |
| 10 | 5218-DELNP-2011-Correspondence Others-(01-08-2011).pdf | 2011-08-01 |
| 11 | 5218-delnp-2011-Form-3-(12-08-2011).pdf | 2011-08-12 |
| 12 | 5218-delnp-2011-Correspondence-Others-(12-08-2011).pdf | 2011-08-12 |
| 13 | Form-5.pdf | 2011-08-21 |
| 14 | Form-3.pdf | 2011-08-21 |
| 15 | Form-1.PDF | 2011-08-21 |
| 16 | Drawings.pdf | 2011-08-21 |
| 17 | 5218-delnp-2011-Form-3-(09-12-2011).pdf | 2011-12-09 |
| 18 | 5218-delnp-2011-Correspondence Others-(09-12-2011).pdf | 2011-12-09 |
| 19 | 5218-delnp-2011-Form-3-(07-06-2012).pdf | 2012-06-07 |
| 20 | 5218-delnp-2011-Correspondence-Others-(07-06-2012).pdf | 2012-06-07 |
| 21 | 5218-delnp-2011-Form-3-(11-06-2013).pdf | 2013-06-11 |
| 22 | 5218-delnp-2011-Correspondence-Others-(11-06-2013).pdf | 2013-06-11 |
| 23 | 5218-delnp-2011-PCT-311.pdf | 2015-09-22 |
| 24 | 5218-delnp-2011-PCT-308.pdf | 2015-09-22 |
| 25 | 5218-delnp-2011-PCT-301.pdf | 2015-09-22 |
| 26 | 5218-delnp-2011-PCT-210.pdf | 2015-09-22 |
| 27 | 5218-delnp-2011-Correspondence Others-(16-10-2015).pdf | 2015-10-16 |
| 28 | 18-delnp-2011-PCT-Document.pdf | 2015-10-23 |
| 29 | 18-delnp-2011-PCT-306.pdf | 2015-10-23 |
| 30 | 18-delnp-2011-PCT-304.pdf | 2015-10-23 |
| 31 | 18-delnp-2011-PCT-210.pdf | 2015-10-23 |
| 32 | 18-delnp-2011-PCT-113.pdf | 2015-10-23 |
| 33 | OTHERS [22-02-2016(online)].pdf | 2016-02-22 |
| 34 | Examination Report Reply Recieved [22-02-2016(online)].pdf | 2016-02-22 |
| 35 | Description(Complete) [22-02-2016(online)].pdf | 2016-02-22 |
| 36 | 5218-delnp-2011-Translasion of the Examination Reports and Granted Claims-(22-02-2016).pdf | 2016-02-22 |
| 37 | 5218-delnp-2011-Official Text of Notification of Remarks of Third Examination-(22-02-2016).pdf | 2016-02-22 |
| 38 | 5218-delnp-2011-Official Text of Notification of Remarks of Second Examination-(22-02-2016).pdf | 2016-02-22 |
| 39 | 5218-delnp-2011-Official Text of Notification of Remarks of Fourth Examination-(22-02-2016).pdf | 2016-02-22 |
| 40 | 5218-delnp-2011-Official Text of Notification of Remarks of First Examination-(22-02-2016).pdf | 2016-02-22 |
| 41 | 5218-delnp-2011-Official Communication-(22-02-2016).pdf | 2016-02-22 |
| 42 | 5218-delnp-2011-Notification of Submission of Argument-(22-02-2016).pdf | 2016-02-22 |
| 43 | 5218-delnp-2011-Notification of Reasons for Rejection-(22-02-2016).pdf | 2016-02-22 |
| 44 | 5218-delnp-2011-Notification of Decision of Regection-(22-02-2016).pdf | 2016-02-22 |
| 45 | 5218-delnp-2011-Non-final Office Action-(22-02-2016).pdf | 2016-02-22 |
| 46 | 5218-delnp-2011-Marked Claims-(22-02-2016).pdf | 2016-02-22 |
| 47 | 5218-delnp-2011-Korea Granted claims-(22-02-2016).pdf | 2016-02-22 |
| 48 | 5218-delnp-2011-Japanese granted claims-(22-02-2016).pdf | 2016-02-22 |
| 49 | 5218-delnp-2011-Granted Patent Publication-(22-02-2016).pdf | 2016-02-22 |
| 50 | 5218-delnp-2011-Granted claims-(22-02-2016).pdf | 2016-02-22 |
| 51 | 5218-delnp-2011-Form-3-(22-02-2016).pdf | 2016-02-22 |
| 52 | 5218-delnp-2011-Final Office Action-(22-02-2016).pdf | 2016-02-22 |
| 53 | 5218-delnp-2011-EESR-(22-02-2016).pdf | 2016-02-22 |
| 54 | 5218-delnp-2011-Drawings-(22-02-2016).pdf | 2016-02-22 |
| 55 | 5218-delnp-2011-Correspondence Other-(22-02-2016).pdf | 2016-02-22 |
| 56 | 5218-delnp-2011-Claims-(22-02-2016).pdf | 2016-02-22 |
| 57 | 5218-DELNP-2011_EXAMREPORT.pdf | 2016-06-30 |
| 58 | Other Patent Document [11-01-2017(online)].pdf | 2017-01-11 |
| 59 | 5218-DELNP-2011-RELEVANT DOCUMENTS [01-03-2018(online)].pdf | 2018-03-01 |
| 60 | 5218-DELNP-2011-RELEVANT DOCUMENTS [07-03-2019(online)].pdf | 2019-03-07 |
| 61 | 5218-DELNP-2011-RELEVANT DOCUMENTS [09-03-2020(online)].pdf | 2020-03-09 |
| 62 | 5218-DELNP-2011-RELEVANT DOCUMENTS [17-08-2021(online)].pdf | 2021-08-17 |
| 63 | 5218-DELNP-2011-RELEVANT DOCUMENTS [10-09-2022(online)].pdf | 2022-09-10 |
| 64 | 5218-DELNP-2011-RELEVANT DOCUMENTS [21-08-2023(online)].pdf | 2023-08-21 |