DESC:1. TITLE OF THE INVENTION
“Method for Preparing Nanoemulsion Incorporating Quercetin and Curcumin”.

APPLICANT (S)
(a) Names:
1. Preeti
2. Saraf Swarnlata
3. Saraf Shailendra
(b) Nationality; Indian
(c) Address:
1. Preeti
Village-Kalmitar,Post-Ranigaon,Thana-Ratanpur,District-Bilaspur, 495442, Chhattisgarh,India,9340464127,preeti.goldar199@gmail.com
2. Saraf Swarnlata
Q9, Lokmanya Grih Nirman Society, Rohinipuram, Near Gol Chowk Raipur, 492010, Chhattisgarh, India.

3. Saraf Shailendra
Q9, Lokmanya Grih Nirman Society, Rohinipuram, Near Gol Chowk Raipur, 492010, Chhattisgarh, India.
PREAMBLE TO THE DESCRIPTION:
COMPLETE SPECIFICATION
The present invention relates to the “Method for Preparing Nanoemulsion Incorporating Quercetin and Curcumin.”
The main objective of the formulation of the nanoemulsion was to incorporate bioactives for topical application. Nanoemulsions are a promising carrier for the delivery of bioactives because of several advantages, such as their small droplet size, enhanced solubility and stability, transparency, versatility, etc. Bioactives curcumin and quercetin are safe, and a combination of both has a greater synergistic anticancer effect than either compound acting alone. But in order to utilize their synergistic therapeutic potential, we must overcome the issues related to the delivery of these bioactives. The incorporation of quercetin and curcumin in nanoemulsion form was performed to prepare a suitable delivery system and overcome the challenges associated with the delivery of these bioactives. Hence, nanoemulsion will be an ideal delivery system to overcome the above-mentioned problems of bioactives and to utilize their synergistic anticancer effect.
CLAIMS
1. The method for preparing nanoemulsion incorporating quercetin and curcumin for skin cancer treatment will be an excellent nanocarrier for utilizing the synergistic effect of both bioactives.
2. The nanoemulsion of claim 1 will be an ideal delivery system to solve the problems related to the delivery of bioactives.
3. This nanoemulsion will also improve skin hydration and facilitate bioactive permeation through the biological membrane.
4. Combination of both herbal bioactive quercetin and curcumin has synergistic anticancer effects.
ABSTRACT
Curcumin and quercetin, both bioactives, have potential anticancer effects individually. However, the combination of both curcumin and quercetin has a greater synergistic anticancer effect in comparison to a single effect. However, poor aqueous solubility and low bioavailability of both bioactives limit their therapeutic effectiveness. Nanoemulsions are biphasic systems, with one phase dispersed in the other phase in the form of droplets ranging in diameter from 100 to 500 nm. These nano-range droplet delivery systems have the ability to release the bioactives quickly in a free form under dilution in biological fluids. Hence, nanoemulsion loaded with both bioactives will be an ideal delivery system to overcome all problems and will be a fruitful option for the treatment of skin cancer. These nano-range droplet delivery systems have the ability to release the bioactives quickly in a free form under dilution in biological fluids. Hence, nanoemulsion loaded with both bioactives will be an ideal delivery system to overcome all problems and will be a fruitful option for the treatment of skincancer.
BACKGROUND OF THE INVENTION (Details of experimental work)
Description of composition and method of preparation of above said nanoemulsion given below
Curcumin, a bioactive compound found in turmeric, has very potent biological effects, including anti-cancer, antioxidant, and anti-inflammatory effects. Quercetin is also a bioactive compound found in various fruits, vegetables, grains, etc. Similar to curcumin, quercetin also has anticancer, antioxidant, and anti-inflammatory properties. However, the combination of curcumin and quercetin has a synergistic effect, meaning their combined action could be more beneficial than each bioactive alone. Nanotechnology-based topical nanoemulsions will be an ideal bioactive delivery for the combination of both bioactives.
Table1: Formula for Preparation of 100ml of Nanoemulsion
S.N. Ingredients Quantity
1. Distilled water 82ml
2. Tween-80 6ml
3. Polyethylene Glycol-400 2ml
4. Castor oil 10 ml
5. Drug Quercetin(90mg)
Curcumin(90 mg)

Method of Preparation:
The nanoemulsion was prepared via high-speed homogenization of a mixture consisting of a water phase and an oil phase. To prepare the nanoemulsion loaded with curcumin and Quercetin, the oil and aqueous phases of the emulsion were first prepared separately. In order to prepare the aqueous phase, surfactant and co-surfactant were added to distilled water and homogenized for 3 minutes at 5000 rpm using a homogenizer. The oil phase was prepared by adding a binary mixture of both bioactives (1:1) to the oil phase under magnetic stirring at 1500 rpm for 10 minutes. Then both phases were added. The oil phase represents 10% of the final volume of the formulation and is homogenized for 30 minutes at 6000 rpm.
Characterization of Prepared Nanoemulsion:
Droplet Size, Zeta Potential, and Polydispersity Index Measurements:
The average globule size or droplet size provides information about the size of the dispersed phase particles in the nanoemulsion. Zeta potential indicates the electric charge on the surface of the droplets in the nanoemulsion, and the polydispersity index measures the distribution of droplets inside the nanoemulsion. Globule size, zeta potential, and polydispersity index of the prepared nanoemulsion were carried out by Malvern-Zetasizer via a Nano ZS90 (dynamic light scattering technique).
SUMMARY OF THE INVENTION (CONCLUSION)
It’s concluded that the claimed nanoemulsion incorporating both bioactives was successfully prepared through high-speed homogenization techniques. The composition and method of preparation were found to be suitable for preparing nanoemulsion. Some relevant studies were carried out to evaluate the prepared nanoemulsion. As per the data of the evaluation studies, it is concluded that quercetin and curcumin-loaded nanoemulsion have average globule or droplet sizes of 201 nm (around 200 nm) and are suitable for enhanced permeability through the stratum corneum (outermost layer of skin). Zeta potentials of 18.7 mV suggest good stability of the nanoemulsion. Polydispersity Index 0.291 indicates a uniform level of particle size distribution and good consistency in nanoemulsion. Therefore, this topical nanoemulsion loaded with both bioactives may be an ideal delivery system for skin cancer treatment
,CLAIMS:CLAIMS
1. The method for preparing nanoemulsion incorporating quercetin and curcumin for skin cancer treatment will be an excellent nanocarrier for utilizing the synergistic effect of both bioactives.
2. The nanoemulsion of claim 1 will be an ideal delivery system to solve the problems related to the delivery of bioactives.
3. This nanoemulsion will also improve skin hydration and facilitate bioactive permeation through the biological membrane.
4. Combination of both herbal bioactive quercetin and curcumin has synergistic anticancer effects.