Title of Invention: Method for preparing indole or indazole compound
technical field
[One]
Cross Citation with Related Applications
[2]
This application claims the benefit of priority based on Korean Patent Application No. 10-2019-0073017 dated June 19, 2019, and all contents disclosed in the literature of the Korean patent application are incorporated as a part of this specification.
[3]
technical field
[4]
The present invention relates to a method for preparing an indole or indazole compound comprising a nitro group, which is an intermediate structure necessary for synthesizing a pharmaceutically useful indole or indazole compound.
background
[5]
Numerous research results have been reported on compounds having an indole structure as the parent nucleus. For example, patent WO2006/112549 reporting activity against glucokinase, and patent WO1995/007276 reporting useful as an antitumor and cardiovascular inhibitory agent. , and patent WO2004/018428, which reports that it can be used as an antibiotic, are representative.
[6]
Among them, the present invention relates to a method for preparing an indole or indazole compound containing a nitro group as an intermediate structure necessary for obtaining an indole or indazole compound exhibiting a preventive or therapeutic and ameliorating effect on cell necrosis and related diseases. .
[7]
Conventionally, as an intermediate containing an ether group is synthesized during the synthesis of an intermediate containing an indole or indazole compound containing a nitro group from benzoic acid containing a nitro group, a halogenation reaction, a reaction to form an acetylene intermediate, and cyclization from benzoic acid as a starting material When performing the reaction, there was a problem in that the ratio of side reactions was high due to low reactivity, and column purification processes such as filtration and purification using celite were essential at each step in order to remove by-products due to side reactions. As such, when the column purification process is performed, each synthesis step cannot be performed in situ in the same reactor, thereby reducing process efficiency, and the column purification process is difficult to scale up and cannot be applied to the process for mass production. there was
[8]
In addition, when an intermediate including an ether group is used, a process of substituting an ether group with a carboxylic acid must be necessarily accompanied, thereby complicating the process step.
[9]
Accordingly, there is an urgent need for research that can simplify the manufacturing process of the intermediate structure required to obtain an indole or indazole compound that exhibits preventive or therapeutic and ameliorating effects on cell necrosis and related diseases and increase the efficiency of the process.
[10]
[Prior art literature]
[11]
[Patent Literature]
[12]
International Publication WO2006/112549 (2006.10.26)
[13]
International Publication WO1995/007276 (1995.03.16)
[14]
International Publication WO2004/018428 (2004.03.04)
DETAILED DESCRIPTION OF THE INVENTION
technical challenge
[15]
The present invention is an intermediate structure necessary to obtain an indole or indazole compound exhibiting a preventive or therapeutic and ameliorating effect on cell necrosis and related diseases, wherein the indole or indazole compound containing a nitro group is prepared from a benzoic acid compound containing a nitro group In the method, it is possible to simplify a complicated process of more than six conventional steps, increase the reactivity of each intermediate step reaction to increase the yield of the product, and as it becomes possible to replace column purification with solid purification An object of the present invention is to provide a method for preparing an indole or indazole compound containing a nitro group that can be applied to a mass production process.
means of solving the problem
[16]
According to one aspect of the present invention, there is provided a method for preparing a compound represented by Formula 1, comprising the step (step a) of preparing a compound represented by Formula 3 by adding a halogen donor to the compound represented by Formula 2, The halogen donor is N-iodosuccinimide, potassium iodide (KI), iodine (I2), N-bromosuccinimide, N-chlorosuccinimide and N-fluorobenzene-sulfonimide It provides a manufacturing method comprising one or more selected from the group consisting of:
[17]
[Formula 1]
[18]

[19]
In Formula 1,
[20]
n is an integer from 1 to 3,
[21]
m is 0 or 1,
[22]
b is an integer from 1 to 3;
[23]
A represents phenyl or 5 membered heteroaryl or heterocycle each containing 1 to 3 heteroatoms selected from N, O and S atoms and optionally substituted by R, wherein R represents hydrogen, C 1 -C 4 -alkyl optionally substituted by hydroxy or amino ,
[24]
X represents C or N, with the proviso that when X is N, m is 0 and when X is C, m is 1,
[25]
R 1 represents hydrogen, C 1 -C 6 -alkyl or —(CH 2 ) r NR 8 R 9 , wherein r is an integer from 2 to 5, and R 8 and R 9 are each independently hydrogen or C 1 -C 3 -alkyl, with the proviso that when X is N R 1 is hydrogen,
[26]
R 2 represents hydrogen, halogen or C 1 -C 6 -alkoxy, or -(CH 2 ) p C(O) 2 R 8 , -(CH 2 ) p OR 8 , -(CH 2 ) p NR 8 R 9 , -NHR 10 , -N(H)S(O) 2 R 8 or -NHC(O) 2 R 10 , or the heterocycle moiety represents 1 or 2 heteroatoms selected from N, O and S atoms A 5- to 6-membered ring comprising -(CH 2 ) Represents p -heterocycle- R 10 , wherein p is an integer from 0 to 3, R 8 and R 9 are as defined above, R 10 is hydrogen, oxo, C 1 -C 6 -alkylcarbonyl, C 1 -C 6 -alkoxy or C 1 -C 6 -alkyl or 5 to 6 membered heterocycle comprising 1 or 2 nitrogen atoms as heteroatoms,
[27]
R 3 represents hydrogen, halogen, C 1 -C 6 -alkyl or phenyl, or -(CH 2 ) q - wherein the heterocycle contains 1 or 2 heteroatoms selected from N and O atoms and is a 5 to 6 membered ring heterocycle, wherein q is an integer from 1 to 3 with the proviso that when X is N R 3 is hydrogen or phenyl,
[28]
wherein alkyl, alkoxy, aryl, cycloalkyl, heterocycle and heteroaryl may be optionally substituted, the substituents being hydroxy, C 1 -C 6 -alkylamino, di(C 1 -C 6 -alkyl)amino, at least one selected from the group consisting of carboxy, C 1 -C 6 -alkyl, C 1 -C 6 -alkoxy, carboxy-C 1 -C 6 -alkyl and oxo,
[29]
[Formula 2]
[30]

[31]
In Formula 2,
[32]
c is an integer from 0 to 2,
[33]
[Formula 3]
[34]

[35]
In Formula 3, Y is fluoro, chloro, bromo or iodo,
[36]
c is as defined in Formula 2 above.
[37]
[38]
According to another aspect of the present invention, there is provided a method for preparing a compound represented by the formula (6), comprising the step of synthesizing a compound represented by the following formula (6) from the compound represented by the formula (1).
[39]
[Formula 6]
[40]

[41]
In Formula 6,
[42]
R 1 , R 2 , R 3 , n, m are as defined in Formula 1 above,
[43]
R 5 represents hydrogen, C 1 -C 6 -alkyl, C 3 -C 6 -cycloalkyl, heterocycle or heterocyclyl-C 1 -C 6 -alkyl, wherein the heterocycle is selected from N and O atoms It is a 3 to 8 membered ring containing 1 to 3 heteroatoms, with the proviso that when X is N, R 5 is hydrogen,
[44]
R 6 represents -(CR 8 R 9 )uZDWR 14 , where u is an integer of 0 to 3, and Z represents a direct bond or a group consisting of -C(O)- and -C(O)O- is selected from, D represents a direct bond, C 4 -C 6 -cycloalkyl, 5 to 6 membered heteroaryl containing 1 or 2 N atoms, or from N, O and S atoms Represents a 5-6 membered heterocycle containing selected 1 or 2 heteroatoms, W represents a direct bond or -NR 8 -, -C(O)-, -C(O)O-, -C(O) )NR 12 - or -S(O) t -, R 12 represents hydrogen, C 1 -C 3 -alkyl or C 6 -C 10 -aryl, t is an integer of 1 or 2, R 14is a 5 to 6 membered heterocycle comprising 1 to 3 heteroatoms selected from hydrogen, hydroxy, C 1 -C 6 -alkyl, N, O and S atoms, or C 6 -C 10 -ar-C 1 - Represents C 6 -alkyl, with the proviso that when X is N, R 6 represents C 4 -C 6 -cycloalkyl or 5 to 6 containing 1 or 2 heteroatoms selected from N, O and S atoms. represents a one heterocycle,
[45]
R 7 represents -Y'R 11 , wherein Y' is a direct bond or represents -(CR 8 R 9 ) h Y"-, where h is an integer of 0 to 3, R 11 is hydrogen, halogen, C 1 -C 6 -alkyl and -(CH 2 ) v B-R 13 , v is an integer from 0 to 3, and B contains 1 or 2 heteroatoms selected from N, O and S atoms and represents a 5 to 6 membered heterocycle or C 6 -C 10 -aryl, R 13 represents hydrogen, cyano, halogen, hydroxy, oxo, thiol, carboxy or carboxy -C 1 -C 6 -alkyl , R 8, R 9 is as defined in claim 1, and Y" is selected from the group consisting of -O-, -C(O)- and -C(O)O-, with the proviso that when X is N, R 7 is hydrogen or C 1 -C 6 -alkyl,
[46]
wherein alkyl, alkoxy, aryl, cycloalkyl, heterocycle and heteroaryl may be optionally substituted, the substituents being hydroxy, C 1 -C 6 -alkylamino, di(C 1 -C 6 -alkyl)amino, at least one selected from the group consisting of carboxy, C 1 -C 6 -alkyl, C 1 -C 6 -alkoxy, carboxy-C 1 -C 6 -alkyl and oxo.
Effects of the Invention
[47]
The method for producing an indole or indazole compound of the present invention simplifies the process and increases the reactivity of each intermediate step reaction compared to the conventional method for producing an indole or indazole compound containing a nitro group, thereby increasing the yield of the product, and each step Since the ratio of by-products can be lowered by improving the reactivity of each target reaction, the column purification process using Celite can be omitted, thereby greatly improving the efficiency of the process, and as the column purification process is replaced by a solid purification process, It can be easily applied to the process for production.
Modes for carrying out the invention
[48]
Hereinafter, the present invention will be described in more detail to help the understanding of the present invention.
[49]
The terms or words used in the present specification and claims should not be construed as being limited to their ordinary or dictionary meanings, and the inventor may properly define the concept of the term in order to best describe his invention. Based on the principle that there is, it should be interpreted as meaning and concept consistent with the technical idea of ​​the present invention.
[50]
In the definition of the substituent of the compound of formula (1) according to the present invention, the term 'alkyl' means an aliphatic hydrocarbon radical. Alkyl can be “saturated alkyl” containing no alkenyl or alkynyl moieties, or “unsaturated alkyl” containing at least one alkenyl or alkynyl moiety. “Alkenyl” refers to a group containing at least one carbon-carbon double bond, and “alkynyl” refers to a group containing at least one carbon-carbon triple bond. Alkyl, when used alone or in combination with alkoxy, may be branched or straight-chain, respectively.
[51]
Alkyl groups may have from 1 to 20 carbon atoms unless otherwise defined. The alkyl group may be a medium sized alkyl having from 1 to 10 carbon atoms. The alkyl group may be a lower alkyl having 1 to 6 carbon atoms. Typical alkyl groups include, but are not limited to, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, t-butyl, pentyl, hexyl, ethenyl, propenyl, butenyl, and the like. For example, C 1 -C 4 -alkyl has 1 to 4 carbon atoms in the alkyl chain and consists of methyl, ethyl, propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl and t-butyl. selected from the group.
[52]
The term 'alkoxy', unless otherwise defined, means alkyloxy having 1 to 10 carbon atoms.
[53]
The term 'cycloalkyl' means a saturated aliphatic 3-10 membered ring, unless otherwise defined. Typical cycloalkyl groups include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and the like.
[54]
The term 'aryl' includes at least one ring having a shared pi electron system, for example monocyclic or fused-ring polycyclic (i.e., rings that share adjacent pairs of carbon atoms) groups. . That is, unless otherwise defined, in the present specification, aryl means a 4-10 membered, preferably 6-10 membered aromatic monocyclic or multicyclic ring including phenyl, naphthyl, and the like.
[55]
Unless otherwise defined, the term 'heteroaryl' includes 1 to 3 heteroatoms selected from the group consisting of N, O and S, and 3 to 10 membered aromatic that may be fused with benzo or C 3 -C 8 cycloalkyl. ring, preferably a 4 to 8 membered ring, more preferably a 5 to 6 membered ring. Examples of monocyclic heteroaryl include thiazole, oxazole, thiophene, furan, pyrrole, imidazole, isoxazole, isothiazole, pyrazole, triazole, triazine, thiadiazole, tetrazole, oxadia sol, pyridine, pyridazine, pyrimidine, pyrazine and the like. Examples of bicyclic heteroaryls include indole, indoline, benzothiophene, benzofuran, benzimidazole, benzoxazole, benzisoxazole, benzthiazole, benzthiadiazole, benztriazole, quinoline, isoquinoline, purine. , puropyridine and a group similar thereto, but are not limited thereto.
[56]
The term 'heterocycle', unless otherwise defined, contains 1 to 3 heteroatoms selected from the group consisting of N, O and S, may be fused with benzo or C 3 -C 8 cycloalkyl, may be saturated or 1 or It means a 3-10 membered ring containing two double bonds, preferably a 4-8 membered ring, more preferably a 5-6 membered ring. Examples of heterocycles include pyrroline, pyrrolidine, imidazoline, imidazolidine, pyrazoline, pyrazolidine, pyran, piperidine, morpholine, thiomorpholine, piperazine, hydrofuran, and the like. However, it is not limited only to these.
[57]
Other terms and abbreviations used in this specification may be interpreted as meanings commonly understood by those skilled in the art to which the present invention belongs, unless otherwise defined.
[58]
The present invention relates to a method for preparing an indole or indazole compound containing a nitro group represented by the following formula (1), which is an intermediate structure necessary for synthesizing a pharmaceutically useful indole or indazole compound, comprising benzoic acid represented by the following formula (2) A step of preparing a compound represented by Formula 3 by adding a halogen donor to the compound containing the compound (step a), wherein the halogen donor is N-iodosuccinimide, potassium iodide (KI), iodine ( I 2 ), N-bromosuccinimide, N-chlorosuccinimide, and N-fluorobenzene-sulfonimide are characterized as comprising at least one selected from the group consisting of:
[59]
[Formula 1]
[60]

[61]
In Formula 1,
[62]
n is an integer from 1 to 3,
[63]
m is 0 or 1,
[64]
b is an integer from 1 to 3;
[65]
A represents phenyl or 5 membered heteroaryl or heterocycle each containing 1 to 3 heteroatoms selected from N, O and S atoms and optionally substituted by R, wherein R represents hydrogen, C 1 -C 4 -alkyl optionally substituted by hydroxy or amino ,
[66]
X represents C or N, with the proviso that when X is N, m is 0 and when X is C, m is 1,
[67]
R 1 represents hydrogen, C 1 -C 6 -alkyl or —(CH 2 ) r NR 8 R 9 , wherein r is an integer from 2 to 5, and R 8 and R 9 are each independently hydrogen or C 1 -C 3 -alkyl, with the proviso that when X is N R 1 is hydrogen,
[68]
R 2 represents hydrogen, halogen or C 1 -C 6 -alkoxy, or -(CH 2 ) p C(O) 2 R 8 , -(CH 2 ) p OR 8 , -(CH 2 ) p NR 8 R 9 , -NHR 10 , -N(H)S(O) 2 R 8 or -NHC(O) 2 R 10 , or the heterocycle moiety represents 1 or 2 heteroatoms selected from N, O and S atoms A 5- to 6-membered ring comprising -(CH 2 ) Represents p -heterocycle- R 10 , wherein p is an integer from 0 to 3, R 8 and R 9 are as defined above, R 10 is hydrogen, oxo, C 1 -C 6 -alkylcarbonyl, C 1 -C 6 -alkoxy or C 1 -C 6 -alkyl or 5 to 6 membered heterocycle comprising 1 or 2 nitrogen atoms as heteroatoms,
[69]
R 3 represents hydrogen, halogen, C 1 -C 6 -alkyl or phenyl, or -(CH 2 ) q - wherein the heterocycle contains 1 or 2 heteroatoms selected from N and O atoms and is a 5 to 6 membered ring heterocycle, wherein q is an integer from 1 to 3 with the proviso that when X is N R 3 is hydrogen or phenyl,
[70]
wherein alkyl, alkoxy, aryl, cycloalkyl, heterocycle and heteroaryl may be optionally substituted, the substituents being hydroxy, C 1 -C 6 -alkylamino, di(C 1 -C 6 -alkyl)amino, at least one selected from the group consisting of carboxy, C 1 -C 6 -alkyl, C 1 -C 6 -alkoxy, carboxy-C 1 -C 6 -alkyl and oxo,
[71]
[Formula 2]
[72]

[73]
In Formula 2,
[74]
c is an integer from 0 to 2,
[75]
[Formula 3]
[76]

[77]
In Formula 3, Y may be fluoro, chloro, bromo or iodo,
[78]
c is as defined in Formula 2 above.
[79]
[80]
Specifically, in Formula 1, R 1 may be hydrogen, C 1 -C 6 -alkyl, or di(C 1 -C 3 -alkyl)amino-C 2 -C 3 -alkyl.
[81]
In addition, the R 2 is hydrogen, halogen, carboxy, carboxy -C 1 -C 3 -alkyl, C 1 -C 3 -alkoxycarbonyl, C 1 -C 3 -alkoxycarbonyl-C 1 -C 3 -alkyl, hydroxy-C 1 -C 3 -alkyl, C 1 -C 3 -alkoxy, -(CH 2 ) p NR 8 R 9 , -NHR 10 , -N(H)S ( O) 2 R 10　or -NHC(O) 2It may represent R 10 or -(CH 2 ) p -heterocycle-R 10 , wherein heterocycle, p, R 8 , R 9　and R 10 are as described above.
[82]
Furthermore, R 3 represents hydrogen, methyl or halogen, or represents phenyl optionally substituted by C 1 -C 3 -alkoxy, or the heterocycle comprises 1 or 2 heteroatoms selected from N and O atoms and 1 or heterocyclyl-C 1 -C 3 -alkylene, which is a 5 to 6 membered ring optionally substituted by two oxo groups.
[83]
In addition, b may be specifically 1 to 2, more specifically 1.
[84]
In addition, in Formula 2, c may specifically have a value of b-1, more specifically 0 to 1, and even more specifically 0.
[85]
In addition, in Formula 3, Y may be specifically bromo or iodo, more specifically iodo, and c is as defined in Formula 2 above.
[86]
[87]
In addition, the compound represented by Formula 1 may be represented by the following Formula (1a) or (1b):
[88]
[Formula 1a]
[89]

[90]
[Formula 1b]
[91]

[92]
In the above formula, n, A, R 1 , R 2 , R 3 and b are as defined in claim 1.
[93]
[94]
As an example, the compound represented by Formula 1 or Formula 1a may be 7-nitro-2-phenyl-1H-indol-5-yl)methanol, and the compound represented by Formula 2 may be 4-amino-3- It may be nitrobenzoic acid.
[95]
[96]
Further, according to an embodiment of the present invention, the halogen donor in step a) may preferably be N-bromosuccinimide or N-iodosuccinimide.
[97]
[98]
In addition, step a) may be performed in the presence of an acid catalyst. Here, the acid catalyst may include at least one of sulfuric acid, nitric acid, acetic acid, trifluoroacetic acid or hydrochloric acid, and preferably, sulfuric acid.
[99]
In addition, the step a) may be to add the compound represented by Formula 2, a halogen donor, in the presence of an acid catalyst at a temperature of 0 to 25 ℃, after the addition, the temperature is raised to 60 to 120 ℃, and then 2 to 10 hours It may be to proceed with the halogenation reaction of the compound represented by the formula (2) by stirring for a while.
[100]
[101]
In addition, according to an embodiment of the present invention, the step (step b) of adding a compound represented by the following formula (4) after step a) may be further included.
[102]
[Formula 4]
[103]

[104]
In Formula 4,
[105]
R 2 , A, n and m are as defined in Formula 1 above,
[106]
R 4 may be acetylene.
[107]
[108]
Step b) of the present invention may be a linking reaction of linking the acetylene compound represented by Formula 4 to the halogenated benzoic acid compound, and specifically, step b) may be performed in the presence of a metal catalyst, in which case the metal catalyst may include a metal such as Cu(I) and Pd(II), and the metal catalyst may be a compound in the form of a halide including the metal specifically. For example, the metal catalyst may be CuI, Pd(PPh 3 ) 2 Cl 2 .
[109]
In addition, step b) may be performed for 2 to 10 hours at a temperature of 30 to 100 °C.
[110]
[111]
In addition, according to an embodiment of the present invention, after step b), adding a base in the presence of a metal catalyst may further include a step (step c) of preparing a compound represented by the following Chemical Formula 5 (step c), at this time Step b) and step c) may be performed in the same reactor (in-situ reaction).
[112]
[Formula 5]
[113]

[114]
In Formula 5, R 1 to R 3 , A, X, n and m are as defined in Formula 1 above, and c is as defined in Formula 2 above.
[115]
[116]
In the present invention, step b), which is a linking reaction, and step c), which is a cyclization reaction to form indole or indolane, can be continuously performed in the same reactor, and the compound represented by Formula 3 is represented by Formula 4 The intermediate compound formed by reacting with the compound can be directly reacted with a base without going through a separate work-up or purification process after synthesis to prepare the compound represented by Formula 5, and a separate work-up or purification process can be performed. Since the compound represented by Formula 5 in high yield can be obtained even without going through, the efficiency of the process can be greatly improved.
[117]
In step c), the base is, for example, potassium t-butoxide (KOBu t ), potassium carbonate (K 2 CO 3 ), DBU (1,8-Diazabicyclo[5.4.0]undec-7-ene), triethylamine ( Et 3 N), diisopropylethylamine (EtN(iPr) 2- ), N-methyl morpholine, methyl pyrrolidone, and may be at least one of N-methylpropionamide (NMPA), preferably DBU may include
[118]
In addition, the step c) may be performed for 10 to 30 hours, preferably from 15 to 20 hours, by raising the temperature to 50 to 150° C. after adding the cheap price.
[119]
[120]
In addition, according to an embodiment of the present invention, the preparation method may further include a step (step d) of preparing the compound represented by Formula 1 by adding a reducing agent after step c). In this case, step d) may be to prepare a compound represented by Formula 1 through a reduction reaction.
[121]
In addition, the reducing agent may be at least one of BH 3 -DMS (borane dimethyl sulfide), NaBH 4 , diisobutylaluminum hydride (DIBAL) and lithium aluminum hydride (LAH), and preferably BH 3 -DMS (borane phosphorus dimethyl sulfide).
[122]
In addition, in step d), the reducing agent may be added at a temperature of 0 to 30 ℃, specifically, may be added at 5 to 30 ℃, or 10 to 15 ℃. In addition, after the addition of the reducing agent, the temperature may be raised to 10 to 80 °C, and stirred for 2 to 10 hours to synthesize the compound represented by Chemical Formula 1.
[123]
[124]
In addition, according to another embodiment of the present invention, the present invention may include a method for preparing a compound represented by the formula (6), including the step of synthesizing a compound represented by the following formula (6) from the compound represented by the formula (1) can
[125]
[Formula 6]
[126]

[127]
In Formula 6,
[128]
R 1 , R 2 , R 3 , n, m are as defined in Formula 1 above,
[129]
R 5 represents hydrogen, C 1 -C 6 -alkyl, C 3 -C 6 -cycloalkyl, heterocycle or heterocyclyl-C 1 -C 6 -alkyl, wherein the heterocycle is selected from N and O atoms It is a 3 to 8 membered ring containing 1 to 3 heteroatoms, with the proviso that when X is N, R 5 is hydrogen,
[130]
R 6 represents -(CR 8 R 9 )uZDWR 14 , where u is an integer of 0 to 3, and Z represents a direct bond or a group consisting of -C(O)- and -C(O)O- is selected from, D represents a direct bond, C 4 -C 6 -cycloalkyl, 5 to 6 membered heteroaryl containing 1 or 2 N atoms, or from N, O and S atoms Represents a 5-6 membered heterocycle containing selected 1 or 2 heteroatoms, W represents a direct bond or -NR 8 -, -C(O)-, -C(O)O-, -C(O) )NR 12 - or -S(O) t -, R 12 represents hydrogen, C 1 -C 3 -alkyl or C 6 -C 10 -aryl, t is an integer of 1 or 2, R 14is a 5 to 6 membered heterocycle comprising 1 to 3 heteroatoms selected from hydrogen, hydroxy, C 1 -C 6 -alkyl, N, O and S atoms, or C 6 -C 10 -ar-C 1 - Represents C 6 -alkyl, with the proviso that when X is N, R 6 represents C 4 -C 6 -cycloalkyl or 5 to 6 containing 1 or 2 heteroatoms selected from N, O and S atoms. represents a one heterocycle,
[131]
R 7 represents -Y'R 11 , wherein Y' is a direct bond or represents -(CR 8 R 9 ) h Y"-, where h is an integer of 0 to 3, R 11 is hydrogen, halogen, C 1 -C 6 -alkyl and -(CH 2 ) v B-R 13 , v is an integer from 0 to 3, and B contains 1 or 2 heteroatoms selected from N, O and S atoms and represents a 5 to 6 membered heterocycle or C 6 -C 10 -aryl, R 13 represents hydrogen, cyano, halogen, hydroxy, oxo, thiol, carboxy or carboxy -C 1 -C 6 -alkyl , R 8, R 9 is as defined in claim 1, and Y" is selected from the group consisting of -O-, -C(O)- and -C(O)O-, with the proviso that when X is N, R 7 is hydrogen or C 1 -C 6 -alkyl,
[132]
wherein alkyl, alkoxy, aryl, cycloalkyl, heterocycle and heteroaryl may be optionally substituted, the substituents being hydroxy, C 1 -C 6 -alkylamino, di(C 1 -C 6 -alkyl)amino, at least one selected from the group consisting of carboxy, C 1 -C 6 -alkyl, C 1 -C 6 -alkoxy, carboxy-C 1 -C 6 -alkyl and oxo.
[133]
In addition, the method of the present invention may further include a step of purifying after synthesizing each product in step a), step c), and step d), in which case the purification is to be performed by a solid purification method. can
[134]
Specifically, solid purification according to an embodiment of the present invention may be performed by adding water, a polar organic solvent, or a mixture thereof to the compound synthesized for each step to form a solid, and then filtering and washing the solid. . In this case, the polar organic solvent may specifically include at least one of alcohol and dichloromethane, and more specifically, the alcohol may include at least one of methanol, ethanol, propanol, and butanol.
[135]
In addition, before adding water, a polar organic solvent, or a mixture thereof to the compound, a compound concentrate can be obtained first by distillation under reduced pressure, and, if necessary, the temperature of the reactor containing the compound before distillation under reduced pressure is adjusted to room temperature (25±5° C.) ) may further comprise a step of cooling.
[136]
[137]
Hereinafter, the present invention will be described in more detail by way of Examples. However, the following examples are intended to illustrate the present invention, and the scope of the present invention is not limited thereto.
[138]
[139]
Example
[140]
[141]
Example 1: Synthesis of (7-nitro-2-phenyl-1H-indol-5-yl)methanol)
[142]
[143]
1) Synthesis of (4-amino-3-iodo-5-nitrobenzoic acid)
[144]
Commercially purchased (4-amino-3-nitrobenzoic acid) (manufactured by Sinochem Ningbo, China) (5.0 kg), NIS (N-Iodosuccinimide, 9.3 kg), H 2 SO 4 (0.50 kg) and THF (25.0 L) was put into the reactor at room temperature and stirred, then the temperature of the reactor was heated to 80 °C, and then stirred for 2 hours. At this time, the reaction was terminated when the result of analyzing the reaction mixture by HPLC was 5.0% or less of the peak of (4-amino-3-nitrobenzoic acid).
[145]
After confirming the completion of the reaction, the temperature of the reactor was cooled to room temperature, and then dichloromethane (DCM, Dichloromethane, 50.0 L) was added to the concentrated solution obtained by distillation under reduced pressure to form a solid. The formed solid was stirred at room temperature for at least 1 hour, followed by filtration and washing [1st: DCM (40.0 L), 2nd: H 2 O (30.0 L), 3rd: EtOH/H 2 O= 3/7, 30.0 L] It was dried under N 2 pressure for 16 hours to synthesize (4-amino-3-iodo-5-nitrobenzoic acid) (7.6 kg, Yield: 89.8%, Purity: 96.4%).
[146]
1H NMR (500MHz, DMSO-d6) δ 13.1 (br s, 1H), 8.52 (d, J=1.8 Hz, 1H) 8.36 (d, J=1.8 Hz, 1H), 7.50 (s, 2H).
[147]
[148]
2) Synthesis of (7-nitro-2-phenyl-1H-indole-5-carboxylic acid)
[149]
1) of (4-amino-3-iodo-5-nitrobenzoic acid) (7.6 kg), triethylamine (TEA, 7.5 kg), phenylacetylene (Phenylacetylene, 3.0 kg) and 1,4-dioxane (1,4-Dioxane, 76.0 L) was put into the reactor at room temperature and stirred, and then CuI [Copper(I) Iodide, 47.0 g] and Pd(PPh 3 ) 2 Cl 2 (173.0 g), which are reaction catalysts, were added to the reactor. was heated to 60 °C. The reaction mixture was stirred for 2 h. Thereafter, the reaction was terminated when the result of analyzing the reaction mixture by HPLC was 1.0% or less of the peak of (4-amino-3-iodo-5-nitrobenzoic acid).
[150]
Thereafter, DBU (1,8-Diazabicyclo[5.4.0]undec-7-ene, 22.5 kg) was added to the reaction mixture, the temperature of the reactor was heated to 110° C., and the reaction was terminated after stirring for 18 hours. After the reaction mixture was cooled to room temperature, the pH was adjusted to 3 with 3N-HCl aqueous solution (114.0 L), H 2 O (38.0 L) was added, and the mixture was stirred for 2 hours or more, followed by filtration and washing [1st: H 2 O (38.0 L)) , 2nd: EtOH/H 2 O = 3/7, 23.0 L] and then dried under N 2 pressure for 16 hours (7-nitro-2-phenyl-1H-indole-5-carboxylic acid) (6.1 Kg, Yield: 87.4 %, Purity: 98.0% PAR) was synthesized.
[151]
1H NMR (500MHz, DMSO-d6) δ 11.6 (s, 1H), 8.03 (s, 1H), 7.96 (m, 3H), 7.46 (t, J= 7.7 Hz, 2H) 7.36 (d, J= 7.3 Hz) , 1H), 7.10 (s, 1H)
[152]
[153]
3) Synthesis of ((7-nitro-2-phenyl-1H-indol-5-yl)methanol)
[154]
(7-nitro-2-phenyl-1H-indole-5-carboxylic acid) (5.8 kg) of 2) and tetrahydrofuran (THF, 61 L) of 2) were put into a reactor at room temperature, stirred, and the reaction mixture was After cooling to 12±2 ℃, 5 M borane dimethyl sulfide (BH 3 -DMS; Borane dimethyl sulfide, 12.3 L) was slowly added dropwise so that the internal temperature did not exceed 40 ℃. After the dropwise addition was completed and the temperature of the reactor was heated to 40 °C, the reaction mixture was stirred for 2 hours, and then the reaction mixture was analyzed by HPLC (7-nitro-2-phenyl-1H-indole-5-carboxylic acid) ), the reaction was terminated when it was 1.0% or less of the peak.
[155]
After confirming the completion of the reaction, H 2 O (55 L) was slowly added dropwise to the reaction mixture, the layers were separated to store the organic layer, and the water layer was extracted with ethyl acetate (EtOAc; ethyl acetate, 29 L) and mixed with the stored organic layer under reduced pressure. distilled. An ethanol/water mixed solvent (ethanol:6.1 L/water: 12.2 L) was added to the obtained concentrate, stirred for 2 hours or more, and then filtered. After washing using an ethanol/water mixed solvent (EtOH/H 2 O=1/3, 9.0 L) as a washing solution, then drying under N 2 pressure for 16 hours ((7-nitro-2-phenyl-1H-indole- 5-yl) methanol) (4.6 kg, Yield: 83.5%, Purity: 98.4%) was synthesized.
[156]
1H NMR (500MHz, DMSO-d6) δ 11.6 (s, 1H), 8.07 (s, 1H), 8.00 (m, 3H), 7.49 (t, J= 7.7 Hz, 2H) 7.40 (d, J= 7.3 Hz) , 1H), 7.14 (s, 1H), 5.42 (t, J= 6.7 Hz, 1H), 4.66 (d, J= 6.7 Hz, 2H).
Claims
[Claim 1]
A method for preparing a compound represented by Formula 1, comprising adding a halogen donor to the compound represented by Formula 2 to prepare a compound represented by Formula 3 (step a), wherein the halogen donor is N-iodo At least one selected from the group consisting of succinimide , potassium iodide (KI), iodine (I 2 ), N-bromosuccinimide, N-chlorosuccinimide and N-fluorobenzene-sulfonimide A manufacturing method comprising: [Formula 1] In Formula 1, n is an integer of 1 to 3, m is 0 or 1, b is an integer of 1 to 3, A represents phenyl, or each 5 membered heteroaryl or heterocycle comprising 1 to 3 heteroatoms selected from N, O and S atoms and optionally substituted by R, wherein R represents hydrogen or optionally substituted by hydroxy or amino C 1 -C 4 -alkyl, X represents C or N, with the proviso that when X is N m is 0 and when X is C m is 1 and R 1 is hydrogen, C 1 -C 6 - alkyl or -(CH 2 ) r NR 8R 9 , wherein r is an integer from 2 to 5, R 8 and R 9 each independently represent hydrogen or C 1 -C 3 -alkyl, with the proviso that when X is N, R 1 is hydrogen, and R 2 represents hydrogen, halogen or C 1 -C 6 -alkoxy, or -(CH 2 ) p C(O) 2 R 8 , -(CH 2 ) p OR 8 , -(CH 2 ) p NR 8 R 9 , -NHR 10, -N(H)S(O) 2 R 8 or -NHC(O) 2 R 10 , or 5 to 6 heterocycle moieties comprising 1 or 2 heteroatoms selected from N, O and S atoms Represents a membered ring -(CH 2 ) p -heterocycle- R 10 , wherein p is an integer of 0 to 3, R 8 and R 9 are as defined above, and R 10 is hydrogen, oxo, C 1 - C 6 -alkylcarbonyl, C 1 -C 6 -alkoxy or C 1 -C 6 -alkyl or 5 to 6 membered heterocycle containing 1 or 2 nitrogen atoms as heteroatoms, R 3 is hydrogen , halogen, C 1 -C 6 -alkyl or phenyl, or -(CH 2 ) q -heterocycle, wherein the heterocycle contains 1 or 2 heteroatoms selected from N and O atoms and is a 5 to 6 membered ring , wherein q is an integer from 1 to 3, with the proviso that when X is N, R 3 is hydrogen or phenyl, wherein alkyl, alkoxy, aryl, cycloalkyl, heterocycle and heteroaryl may be optionally substituted, and the substituents are hydrogen hydroxy, C 1 -C 6 -alkylamino, di(C 1 -C 6 -alkyl)amino, carboxy, C 1 -C 6 -alkyl, C 1 -C 6 -alkoxy, carboxy-C 1 -C 6 -alkyl and at least one selected from the group consisting of oxo, [Formula 2] In Formula 2, c is an integer of 0 to 2, [Formula 3] In Formula 3, Y is fluoro, chloro, bromo or iodo, and c is as defined in Formula 2 above.
[Claim 2]
The method according to claim 1, wherein the halogen donor is N-bromosuccinimide or N-iodosuccinimide.
[Claim 3]
The method according to claim 1, wherein after step a), adding a compound represented by the following Chemical Formula 4 in the presence of a metal catalyst (step b) further comprises. [Formula 4] In Formula 4, R 2 , A, n and m are as defined in Formula 1, and R 4 is acetylene.
[Claim 4]
The method according to claim 3, further comprising the step of preparing a compound represented by the following formula 5 by adding a base in the presence of a metal catalyst after step b) (step c), wherein step b) and step c) are A manufacturing method that is carried out in the same reactor. [Formula 5] In Formula 5, R 1 to R 3 , A, X, n and m are as defined in Formula 1, and c is as defined in Formula 2 above.
[Claim 5]
According to claim 4, wherein the base is potassium t-butoxide (KOBu t ), potassium carbonate (K 2 CO 3 ), DBU (1,8-Diazabicyclo[5.4.0]undec-7-ene), triethylamine (Et 3 N), diisopropylethylamine (EtN(iPr) 2- ), N-methyl morpholine, methyl pyrrolidone and N-methylpropionamide (NMPA) containing at least one selected from the group consisting of A method for manufacturing phosphorus.
[Claim 6]
5. The method of claim 4, further comprising the step (step d) of preparing a compound represented by Formula 1 by adding a reducing agent after step c).
[Claim 7]
The method according to claim 1, wherein the compound represented by Formula 1 is represented by the following Formula (1a) or (1b): [Formula 1a] [Formula 1b] Wherein , n, A, R 1 , R 2 , R 3 and b are as defined in claim 1.
[Claim 8]
The compound of claim 1, wherein the compound represented by Formula 1 is (7-nitro-2-phenyl-1H-indol-5-yl)methanol, and the compound represented by Formula 2 is 4-amino-3-nitrobenzoic acid. A manufacturing method that is
[Claim 9]
A method for preparing a compound represented by Formula 6 comprising the step of synthesizing a compound represented by Formula 6 from the compound represented by Formula 1 prepared according to claim 1 : [Formula 6] In Formula 6, R 1 , R 2 , R 3 , n, m are as defined in Formula 1 above, and R 5 is hydrogen, C 1 -C 6 -alkyl, C 3 -C 6 -cycloalkyl, heterocycle or heterocyclyl-C 1 - C 6 -alkyl, wherein heterocycle is a 3 to 8 membered ring comprising 1 to 3 heteroatoms selected from N and O atoms, with the proviso that when X is N then R 5 is hydrogen and R 6 is -(CR 8 R 9 )uZDWR 14represents, wherein u is an integer from 0 to 3, Z represents a direct bond or is selected from the group consisting of -C(O)- and -C(O)O-, D represents a direct bond, C 4 -C 6 -cycloalkyl, or 5 to 6 membered heteroaryl containing 1 or 2 N atoms, or 5 to 6 containing 1 or 2 heteroatoms selected from N, O and S atoms represents a 6-membered heterocycle, W represents a direct bond or -NR 8 -, -C(O)-, -C(O)O-, -C(O)NR 12 - or -S(O) t - represents, R 12 represents hydrogen, C 1 -C 3 -alkyl or C 6 -C 10 -aryl, t is an integer of 1 or 2, and R 14 is hydrogen, hydroxy, C 1 -C 6-alkyl, 5 to 6 membered heterocycle comprising 1 to 3 heteroatoms selected from N, O and S atoms, or C 6 -C 10 -ar-C 1 -C 6 -alkyl, provided that X When is N, R 6 represents C 4 -C 6 -cycloalkyl or a 5 to 6 membered heterocycle comprising 1 or 2 heteroatoms selected from N, O and S atoms, and R 7 is -Y Represents 'R 11 , wherein Y' is a direct bond or represents -(CR 8 R 9 ) h Y"-, where h is an integer from 0 to 3, and R 11 is hydrogen, halogen, C 1 -C 6 -alkyl and -(CH 2 ) vBR 13 is selected from the group consisting of, v is an integer from 0 to 3, B is a 5 to 6 membered heterocycle containing 1 or 2 heteroatoms selected from N, O and S atoms, or C 6 -C Represent 10 -aryl, R 13 represents hydrogen, cyano, halogen, hydroxy, oxo, thiol, carboxy or carboxy -C 1 -C 6 -alkyl, R 8 , R 9 are as defined in claim 1, , Y" is selected from the group consisting of -O-, -C(O)- and -C(O)O-, with the proviso that when X is N R 7 represents hydrogen or C 1 -C 6 -alkyl , wherein alkyl, alkoxy, aryl, cycloalkyl, heterocycle and heteroaryl may be optionally substituted, the substituents being hydroxy, C 1 -C 6 -alkylamino, di(C 1 -C 6 -alkyl)amino, carboxy, C 1 -C 6 -alkyl, C 1 -C 6 -alkoxy, carboxy-C 1 -C 6 -alkyl and oxo.