Title of the Invention: A method for recovering and reusing phosphoric acid from fermentation broth or waste fermentation broth
Technical Field
[One]
The present application relates to a method for recovering phosphoric acid from a fermentation broth or a waste liquid thereof and a method for reusing the recovered phosphoric acid for fermentation.
[2]
Background
[3]
In some cases, phosphoric acid is necessary to produce the target substance through fermentation. For example, International Publication WO 2014/182125 mentions that a source of phosphoric acid can be provided for O-phosphoserine fermentation, which is a precursor used in the production of L-cysteine. In addition, WO 2014/064244 discloses a method for producing methionine from sulfides and converting enzymes from O-phosphohomoserine, from which fermentative production of the precursor O-phosphohomoserine is performed. It can be seen that phosphate is essential as a source of phosphorus. Therefore, a plan for recovering the residual phosphoric acid from the fermentation broth containing a large amount of phosphate or its waste liquid and reusing it as a phosphorus source should be devised.
[4]
Conventional phosphoric acid recovery process is generally separated from phosphoric acid-containing ore material by organic solvent extraction process, and then neutralized with calcium salt, ammonium salt, potassium salt, sodium salt, etc. to recover phosphoric acid (US3375068). , US3466141A, US4543239, EP0087323A1, US7687046, US8658117). However, the process of extracting the organic solvent is costly due to the use of solvents and the recovery of the solvent is added, the process is complicated and economical, and the usable organic solvent can act as a toxin in the fermentation process even in a small amount, it is difficult to apply to the fermentation process. On the other hand, there is no patent or reference to a process for recovering or producing phosphoric acid by a method other than an organic solvent extraction method in a fermentation process waste liquid which is not a phosphoric acid-containing ore material.
[5]
Detailed description of the invention
Technical challenges
[6]
The present inventors have made intensive efforts to develop a method for recovering phosphoric acid from a fermentation broth or a fermentation liquor. This application was completed by confirming that a fermentation product in an amount similar to that of pure phosphoric acid was obtained.
[7]
Challenge solution
[8]
One object of the present application is to provide a method for recovering and reusing phosphoric acid from fermentation broth or fermentation broth.
[9]
Another object of the present disclosure is to provide phosphoric acid as an O-phosphoserine (OPS) fermentation broth; Or it provides a method for recovery and reuse from the fermentation waste liquid from which cysteine ​​or derivatives thereof are removed.
[10]
Another object of the present invention is to provide a phosphoric acid is O-phosphohomoserine (OPHS) fermentation broth; Or it provides a method for recovery and reuse from the fermentation waste liquid from which methionine or derivatives thereof are removed.
[11]
Yet another object of the present application is to provide a method for recovering phosphoric acid from a fermentation wastewater containing phosphoric acid.
[12]
Effects of the Invention
[13]
When phosphoric acid is recovered in a fermentation process that is not an ore material, a method of recovering phosphoric acid by a method other than an organic solvent extraction method and reusing the fermentation process is first disclosed herein. The method for recovering and reusing phosphoric acid from the fermentation medium of the present application can recover phosphoric acid from fermentation broth or fermentation broth and reuse it in the fermentation process without using an organic solvent, which can act as a toxin in the fermentation process, which is a problem of organic solvents. There is no problem in that the point, the additional cost of using the solvent, and the complicated process due to the addition of the solvent recovery process, it can be very useful in the fermentation process.
[14]
Brief description of the drawings
[15]
Figure 1 shows briefly the process of recovering phosphate from the fermentation broth or the fermentation broth containing phosphoric acid.
[16]
Figure 2 is a fermentation broth produced by using an enzyme conversion reaction from O-phosphoserine (O-phosphoserine (OPS) or O-phosphohomoserine (OPHS) fermentation broth; Or a process of recovering phosphoric acid from the fermentation waste liquid remaining after removing cysteine, cysteine ​​derivative, methionine or methionine derivative from the fermentation broth, and using the phosphoric acid recovery material for O-phosphoserine or O-phosphohomoserine fermentation. It is shown briefly.
[17]
3 is a fermentation broth produced using an enzyme conversion reaction from O-phosphoserine or O-phosphohomoserine fermentation broth; Or from the fermentation broth remaining after the cysteine, cysteine ​​derivative, methionine or methionine derivative is removed from the fermentation broth, phosphoric acid is recovered in two stages, and the recovered phosphoric acid is again used for O-phosphoserine or O-phosphohomoserine fermentation. The process is shown briefly.
[18]
Best Mode for Carrying Out the Invention
[19]
One aspect of the present application for achieving the above object is (a) concentrating a fermentation broth or fermentation broth containing phosphoric acid (concentration step);
[20]
(b) adjusting the pH of the concentrated concentrate to 8-11 (pH adjustment step);
[21]
(c) crystallizing the phosphate from the pH-adjusted concentrate and separating it from the mother liquor (crystallization step); And
[22]
(d) providing a method for recovering and reusing phosphoric acid from a fermentation broth or a fermentation broth, comprising using the crystallized phosphate as a source of phosphoric acid in a fermentation broth (reuse step).
[23]
[24]
The fermentation broth of step (a) (concentration step) herein is a medium obtained by culturing a microorganism producing a fermentation product in a medium containing phosphoric acid, a culture comprising a microorganism cultured with the medium, or a It may mean an enzyme conversion solution.
[25]
The type of the 'fermentation product' is not limited as long as the fermentation broth contains phosphoric acid. Specifically, the fermentation product may be a target substance, a derivative or a precursor thereof produced by fermentation. In addition, the target material may be an amino acid, for example cysteine ​​or methionine, but is not limited thereto. In addition, the derivative of the target substance may be a derivative of an amino acid, specifically, may be a derivative of cysteine ​​or a derivative of methionine. More specifically, the derivatives of cysteine ​​are N-acetyl-cysteine, cystine, metal-cysteine ​​complexes (e.g. zinc cysteine ​​complexes, manganese cysteine ​​complexes, iron cysteine ​​complexes, copper cysteine ​​complexes, magnesium cysteine ​​complexes, etc.) and cysteine ​​salts (e.g. : Cysteine ​​hydrochloride, and the like) may be one or more selected from, but is not limited thereto. The derivative of methionine may be at least one selected from N-acetyl-methionine, metal-methionine complex (eg, zinc methionine complex, manganese methionine complex, iron methionine complex, copper methionine complex, magnesium methionine complex, etc.), methionine salt, and the like. This is not restrictive. In addition, the precursor of the target material may be a precursor of an amino acid, specifically, may be a precursor of cysteine ​​or a precursor of methionine. More specifically, it may be O-phosphoserine (O-phosphoserine, OPS) or O-phosphohomoserine (O-phosphohomoserine, OPHS), but is not limited thereto.
[26]
In addition, the 'fermentation broth' includes (a) a target substance containing phosphoric acid, a precursor or derivative thereof thereof, (b) a precursor-producing strain, a substrate and a conversion enzyme of the target substance, a target substance or a derivative thereof; And a fermentation broth containing phosphoric acid, or (c) a microorganism expressing a convertase or converting enzyme in a precursor fermentation broth of a target substance; And a target substance or derivative thereof produced by adding a substrate; And fermentation broths containing phosphoric acid, but are not limited thereto.
[27]
More specifically, the fermentation broth is converted based on (i) O-phosphoserine fermentation broth containing phosphoric acid, (ii) O-phosphohomoserine fermentation broth containing phosphoric acid, or (iii) the fermentation broth. Enzymes or microorganisms expressing the same; And amino acids or derivatives thereof prepared by the addition of sulfides; And it may be a fermentation broth containing phosphoric acid. In addition, the (iii) fermentation broth, specifically, O-phosphoserine sulfidylase (O-Phosphoserine sulfhydrylase (OPSS) or microorganisms expressing it; And cysteine ​​or derivatives thereof prepared by addition of sulfides; And it may be a fermentation broth containing phosphoric acid. In addition, the (iii) fermentation broth is O-Phospho-homoserine dependent methionine synthase (O-Phospho-homoserine dependent methionine synthase) or a microorganism expressing it; And methionine or derivatives thereof prepared by the addition of sulfides; And a fermentation broth containing phosphoric acid.
[28]
Herein, the "fermentation waste liquid" may be a liquid in which part or all of the fermentation product is separated from the fermentation broth, but is not limited thereto. Specifically, the fermentation waste liquid may be a solution in which some or all of the amino acids, derivatives or precursors thereof are separated and removed from the fermentation broth containing amino acids, derivatives or precursors thereof, and phosphoric acid, but is not limited thereto. For example, the solution may be a solution in which part or all of an amino acid or a derivative thereof is separated and removed from a fermentation broth including an amino acid, a derivative thereof, and a phosphoric acid. It may be a liquid in which part or all of the amino acid or its derivative is separated and removed from the fermentation broth containing the amino acid, derivative thereof and phosphoric acid. More specifically, for example, O-phosphoserine sulfidylase (O-Phosphoserine sulfhydrylase (OPSS) or a microorganism expressing the same; And cysteine ​​or derivatives thereof prepared by addition of sulfides; And a solution in which part or all of cysteine ​​or a derivative thereof is separated and removed from a fermentation broth containing phosphoric acid, and the O-Phospho-homoserine dependent methionine synthase or O-phosphohomoserine fermentation broth may be expressed. microbe; And methionine or derivatives thereof prepared by adding sulfides; And some or all of methionine or a derivative thereof is separated from the fermentation broth containing phosphoric acid, but is not limited thereto.
[29]
[30]
The 'concentration' of the concentration step herein is not limited to one method capable of increasing the concentration of phosphate ions contained in the fermentation broth or fermentation broth, it may be carried out by a method known in the art. Specifically, the method may be performed by evaporation, heating, depressurization, ventilation, or freezing, but is not limited thereto.
[31]
Concentration herein is carried out to increase the recovery of phosphoric acid, the concentration step is the concentration of phosphate ions of the pH-adjusted concentrate is specifically, 60g / L or more or 70g / L or more, more specifically, 60g / L to 300g / L or The fermentation broth or the fermentation broth may be concentrated to 70 g / L to 250 g / L, but is not limited thereto.
[32]
In addition, the pH of the fermentation broth or fermentation broth may be adjusted herein before the concentration step. Specifically, the pH of the fermentation broth or the fermentation broth may be adjusted to 7 or more, 7.5 or more, 8 or more, 8.5 or more or 9 or more, more specifically, 8 to 11. Adjusting the pH prior to concentration can reduce the content of ammonium ion impurities in the recovered phosphate.
[33]
[34]
(B) step (pH adjustment step) is the step of adjusting the pH of the concentrate before crystallization of phosphate. PH of the concentrate obtained in the concentration step is not limited as long as the phosphate can be crystallized, the pH of the concentrate can be adjusted from neutral to basic, specifically, 7 or more, 7.5 or more, 8 or more, 8.5 or more or 9 or more , More specifically, from 8 to 11.
[35]
In addition, the pH adjustment step herein may be performed by adding a basic substance to the concentrate, specifically, it may be performed by adding a hydroxide. Specifically, the hydroxide may be sodium hydroxide or an aqueous sodium hydroxide solution, but is not limited thereto.
[36]
[37]
Step (c) (crystallization step) herein is the step of recovering phosphate from the pH adjusted concentrate obtained in the pH adjustment step. Recovery of the phosphate herein is performed by producing crystals of the phosphate from the pH adjusted concentrate without using an organic solvent and separating the resulting crystals from the mother liquor.
[38]
Temperature may be adjusted and / or nucleus added for crystallization of the phosphate salt, but is not limited thereto. Specifically for the crystallization of phosphate, between the pH adjustment step and the crystallization step; Or in the crystallization step, further comprising cooling the pH adjusted concentrate. More specifically, the pH adjusted concentrate may be left at room temperature, the pH adjusted concentrate may be cooled before the crystallization step, or the pH adjusted concentrate may be cooled in the crystallization step, but is not limited thereto. On the other hand, the phosphoric acid recovery may be affected by the cooling temperature, but the cooling temperature is not limited as long as crystals of the phosphate can be produced. Specifically, the cooling temperature may be 50 ° C. or less, specifically 0 ° C. to 30 ° C., more specifically 10 ° C. to 20 ° C., but is not limited thereto.
[39]
In addition, specifically, the method may further include adding phosphate crystals as crystal nuclei between the pH adjustment step and the crystallization step or during the crystallization step for crystallization of the phosphate, but is not limited thereto.
[40]
The term 'phosphate' herein means a salt of phosphate or a hydrate thereof, and the type of salt is not limited as long as the phosphoric acid can be recovered through crystallization. Specifically, the phosphate may be at least one selected from trisodium phosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate, and hydrates thereof, and more specifically, may be disodium hydrogen phosphate or a hydrate thereof, but is not limited thereto. The number of water molecules bound to the hydrate is not limited as long as the phosphate hydrate can be crystallized, but specifically 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, Or 13 or more. In addition, the hydrate may specifically be disodium hydrogen phosphate heptahydrate or disodium hydrogen phosphate 12 hydrate, but is not limited thereto.
[41]
Phosphoric acid and phosphate may be used interchangeably herein.
[42]
The method used to separate the phosphate crystals produced herein is not limited as long as it can be solid-liquid separation. Specifically, a centrifuge, a filter press, a compression filter, a rotary vacuum filter, or a membrane separator may be used. This is not restrictive.
[43]
The mother liquid herein refers to a liquid obtained by separating and removing the resulting phosphate crystal from the pH-adjusted concentrate, and may include, but are not limited to, uncrystallized phosphate ions.
[44]
[45]
In order to increase the recovery rate of phosphoric acid, a second crystallization step of recrystallizing phosphate from the mother liquor may be additionally included in one aspect of the method herein. The present invention can obtain high phosphate recovery rate by adding the phosphate crystal obtained in the crystallization step described above (primary recovery phosphate) and the phosphate salt crystal obtained in the secondary crystallization step (secondary recovered phosphate). Specifically, the secondary crystallization step includes (i) concentrating the mother liquor (reconcentrating step), (ii) adjusting the pH of the mother liquor (pH readjustment step), and (iii) from the pH adjusted mother liquor. And crystallizing and separating the phosphate (recrystallization step). Steps (i) to (iii) may be performed in the same manner as the above-described concentration step, pH adjustment step and crystallization step. Specifically, the pH of the mother liquor may be adjusted to 7 or more, 7.5 or more, 8 or more, 8.5 or more or 9 or more, more specifically, 8 to 11.
[46]
Also specifically, the secondary crystallization step may further comprise adding phosphate crystals to the mother liquor as nuclei, for example between the pH readjustment step and the recrystallization step or during the recrystallization step, the phosphate in the mother liquor. The method may further include, but is not limited to, adding crystals to the nucleus.
[47]
In addition, the present application may be carried out two or more times of the crystallization step, in order to increase the phosphoric acid recovery.
[48]
The method herein comprises the pH adjustment step; Or adding an alcohol between the pH adjustment step and the crystallization step. Since alcohol has a low solubility in phosphate, when it is mixed with a phosphate solution, it reduces the phosphate solubility of the solution, thereby increasing the recovery of phosphoric acid. The alcohol is not limited as long as it can increase the recovery rate of phosphoric acid, but may be specifically methanol, ethanol, propanol, butanol or a combination thereof.
[49]
[50]
Step (d) (reuse step) herein is the step of using the phosphate crystals recovered in the crystallization step as phosphoric acid and / or phosphorus sources in various fermentation media requiring phosphoric acid and / or phosphorus sources. The fermentation medium herein is not limited as long as it is a medium used for fermentation requiring phosphoric acid and / or phosphorus sources for the purposes herein, but specifically, fermentation used for the production of O-phosphoserine or O-phosphohomoserine. May be a medium.
[51]
[52]
Another aspect of the present application,
[53]
(A) in the fermentation medium containing phosphoric acid, using a microorganism to produce O-phosphoserine (O-phosphoserine, OPS) (OPS fermentation step);
[54]
(b) O-phosphoserine sulfhydrylase (OPSS) or in the presence of a microorganism expressing the same, O-phosphoserine produced in step (a) by reacting with a sulfide sulfide fermentation solution of cysteine ​​or derivatives thereof Preparing a step (conversion step);
[55]
(c) the fermentation broth; Or concentrating the fermentation waste liquid from which the cysteine ​​or derivative thereof is removed from the fermentation broth (concentration step);
[56]
(d) adjusting the pH of the concentrated concentrate to 8-11 (pH adjustment step);
[57]
(e) crystallizing the phosphate from the pH adjusted concentrate (separation step) from the mother liquor; And
[58]
(f) providing a method for recovering and reusing phosphoric acid from a fermentation broth or a fermentation broth, comprising the step of using the crystallized phosphate as a source of phosphoric acid in a fermentation medium (reuse step).
[59]
[60]
In the present application, step (a) (OPS fermentation step) is a step of producing O-phosphoserine using fermentation medium and microorganisms containing phosphoric acid. O-phosphoserine is an ester of serine and phosphoric acid, and the production of O-phosphoserine requires phosphoric acid. In addition, the microorganism may use a known microorganism capable of producing O-phosphoseline, non-limiting examples, microorganisms with enhanced O-phosphoserine releasing activity (WO 2014/182125, WO 2014/182119) Can be used. In addition, examples of microorganisms with high O-phosphoserine production capacity include, but are not limited to, intrinsic phosphoserine phosphatase (SerB), and / or phosphoglycerate dehydrogenase (SerA) or And microorganisms having enhanced activity of phosphoserine aminotransferase (SerC) (WO 2012/053794), but are not limited thereto.
[61]
[62]
In the present application, step (b) (conversion step) is a step of converting O-phosphoserine obtained in the OPS fermentation step into a cysteine ​​or a derivative thereof under the catalytic action of O-phosphoserine sulfidylase enzyme.
[63]
In addition, the conversion reaction may be performed using the microorganism expressing the enzyme as well as the O-phosphoserine sulfidylase enzyme herein. The enzyme and the microorganism expressing the enzyme can be obtained through means and methods known to those skilled in the art. Specifically, the O-phosphoserine sulfidylase enzyme may be used as enzymes described in WO 2013/089478, WO 2012/053794, WO 2012/053777, but is not limited thereto.
[64]
In addition, the sulfide herein is not limited as long as it can react with O-phosphoserine under the catalytic action of O-phosphoserine sulfidylase enzyme, CH 3 SH, Na 2 S, NaSH, (NH 4 ) 2 S, It may be at least one selected from the group consisting of H 2 S and Na 2 S 2 O 3 .
[65]
[66]
The steps (c) to (e) herein are the same as the above-described concentration step, pH adjustment step, crystallization step.
[67]
[68]
Here, step (f) (reuse step) is a step of using the phosphate crystal recovered in the crystallization step as a phosphoric acid and / or phosphorus source in a fermentation medium requiring phosphoric acid and / or a phosphorus source. Specifically, the fermentation medium may be a fermentation medium used for the production of O-phosphoserine, but is not limited thereto.
[69]
[70]
Another aspect of the present application,
[71]
(a) producing O-phosphohomoserine (OPHS) using a microorganism in a fermentation medium containing phosphoric acid (OPHS fermentation step);
[72]
(b) O-phosphohomoserine dependent methionine synthase or O-phosphohomoserine dependent methionine synthase or in the presence of a microorganism expressing the same, the O-phosphohomoserine produced in step (a) by reacting with sulfide to methionine Or preparing a fermentation broth of a derivative thereof (conversion step);
[73]
(c) the fermentation broth; Or concentrating the fermentation waste liquid from which methionine or its derivatives are removed from the fermentation broth (concentration step);
[74]
(d) adjusting the pH of the concentrated concentrate to 8-11 (pH adjustment step);
[75]
(e) crystallizing the phosphate from the pH adjusted concentrate (separation step) from the mother liquor; And
[76]
(f) providing a method for recovering and reusing phosphoric acid from a fermentation broth or a fermentation broth, comprising the step of using the crystallized phosphate as a source of phosphoric acid in a fermentation medium (reuse step).
[77]
[78]
Step (a) herein (OPHS fermentation step) is a step of producing O-phosphohomoserine using a fermentation medium and microorganisms containing phosphoric acid. O-phosphohomoserine is an ester of threonine and phosphoric acid, and the production of O-phosphohomoserine requires phosphoric acid. In addition, the microorganism may use a known microorganism capable of producing O-phosphohomoserine.
[79]
[80]
The step (b) herein (conversion step) is a step of converting O-phosphohomoserine obtained in the OPHS fermentation step into a methionine or a derivative thereof under the catalytic action of the OPHS dependent methionine synthase.
[81]
In addition, the conversion reaction may be performed using the microorganisms expressing the enzyme as well as the OPHS dependent methionine synthetase. Specifically, the enzyme and the microorganism expressing the enzyme may be obtained through means and methods known to those skilled in the art, for example, but may be obtained through the method disclosed in WO 2014/064244, but is not limited thereto. .
[82]
In addition, the sulfide herein is not limited as long as it can react with O-phosphohomoserine under the catalytic action of OPHS dependent methionine synthase, CH 3 SH, Na 2 S, NaSH, (NH 4 ) 2 S, H It may be at least one selected from the group consisting of 2 S and Na 2 S 2 O 3 .
[83]
[84]
The steps (c) to (e) herein are the same as the above-described concentration step, pH adjustment step, crystallization step.
[85]
[86]
Here, step (f) (reuse step) is a step of using the phosphate crystal recovered in the crystallization step as a phosphoric acid and / or phosphorus source in a fermentation medium requiring phosphoric acid and / or a phosphorus source. Specifically, the fermentation medium may be a fermentation medium used for the production of O-phosphohomoserine, but is not limited thereto.
[87]
[88]
Another aspect of the present disclosure provides a method for recovering phosphoric acid from fermentation waste liquor.
[89]
Specifically, the method herein comprises the steps of (a) concentrating a fermentation waste liquor containing phosphoric acid (concentration step);
[90]
(b) adjusting the concentrated concentrate to pH 8-11 (pH adjustment step);
[91]
(c) crystallizing the phosphate from the pH adjusted concentrate (crystallization step); And
[92]
(d) separating the crystallized phosphate from the mother liquor may be a method of recovering phosphoric acid.
[93]
[94]
The fermentation waste liquid herein is as described above, it may be meant to include enzyme conversion waste liquid.
[95]
The steps (a) to (c) herein are the same as the above-described concentration step, pH adjustment step and crystallization step.
[96]
Herein, the method of separating the phosphate crystal of step (d) (separation step) from the mother liquor is as described in the above-mentioned crystallization step.
[97]
Embodiment for Invention
[98]
The present application is described in detail by the following examples. However, the following examples are merely to illustrate the present application, the scope of the present application is not limited by the following examples.
[99]
[100]
Example 1 Recovery of Phosphate Depending on Concentration of O-Phososerine Fermentation Waste
[101]
[102]
Example 1-1. Phosphoric Acid Recovery Using O-Phososerine Fermentation Waste
[103]
[104]
After culturing a microorganism capable of producing O-phosphoserine (OPS) in a fermentation medium containing phosphoric acid to obtain an O-phosphoserine fermentation broth, the fermentation broth was O-Phosphoserine sulfhydrylase (OPSS) was used to react with the sulfide to obtain a fermentation broth comprising cysteine ​​or cystine (WO 2012/053794). Cysteine ​​or cystine was crystallized from the fermentation broth and solid-liquid separated to obtain a fermentation effluent.
[105]
The ionic composition (g / L) of the O-phosphoserine fermentation effluent was 24.4 sodium ions, 6.4 ammonium ions, 14.8 chlorine ions, 3.4 sulfate ions, 34.3 phosphate ions. 1665 ml of water were evaporated from 2000 ml of initial waste liquor and 50% (w / w) aqueous sodium hydroxide solution (38.0 ml) was added until the pH reached 9.00. The phosphate ion concentration of this solution was 185.4 g / L. Cooling proceeded to 15 ° C., disodium hydrogen phosphate heptahydrate was obtained. The slurry was filtered through a centrifugal basket filter and washed with pure water.
[106]
The final product weighed 159.5 g. The ion composition (g / kg) of the final product was 124.6 sodium ions, 5.3 ammonium ions, 5.4 chlorine ions, 4.2 sulfate ions, 388.5 phosphate ions. The moisture content of the final product was 45.7 wt%. Total phosphoric acid recovery was 79.2 wt%.
[107]
[108]
Example 1-2. Phosphoric Acid Recovery Using O-Phososerine Fermentation Waste
[109]
[110]
The O-phosphoserine fermentation based process waste liquor ion composition (g / L) of this example was 24.7 sodium ions, 6.3 ammonium ions, 16.4 chlorine ions, 3.9 sulfate ions, 35.8 phosphate ions. 1555 ml of water was evaporated from 1945 ml of initial waste liquor and 50% aqueous sodium hydroxide solution (46.5 ml) was added until the pH reached 9.00. The phosphate ion concentration of this solution was 159.7 g / L. Cooling proceeded to 15 ° C., disodium hydrogen phosphate heptahydrate was obtained. The slurry was filtered through a centrifugal basket filter and washed with pure water.
[111]
The final product weighed 132.3 g. The ion composition (g / kg) of the final product was 127.4 sodium ions, 10.9 ammonium ions, 5.2 chlorine ions, 3.1 sulfate ions, 358.6 phosphate ions. The moisture content of the final product was 49.6 wt%. Total phosphoric acid recovery was 68.0 wt%.
[112]
[113]
Example 1-3. Phosphoric Acid Recovery Using O-Phososerine Fermentation Waste
[114]
[115]
The O-phosphoserine fermentation based process effluent ion composition (g / L) of this example was 24.6 sodium ions, 6.2 ammonium ions, 16.3 chlorine ions, 3.5 sulfate ions, 35.6 phosphate ions. 1315 ml of water was evaporated in 1975 ml of initial waste liquor and 50% aqueous sodium hydroxide solution (43.0 ml) was added until the pH reached 9.01. The phosphate ion concentration of this solution was 99.9 g / L. Cooling proceeded to 15 ° C., disodium hydrogen phosphate 12-hydrate was obtained. The slurry was filtered through a centrifugal basket filter and washed with pure water.
[116]
The final product weighed 186.0 g. The ionic composition (g / kg) of the final product is 110.7 sodium ions, 13.5 ammonium ions, 2.4 chlorine ions, 2.8 sulfate ions, 264.7 phosphate ions. The moisture content of the final product was 60.8 wt%. Total phosphoric acid recovery was 70.1 wt%.
[117]
[118]
Example 1-4. Phosphoric Acid Recovery Using O-Phososerine Fermentation Waste
[119]
[120]
The O-phosphoserine fermentation based process waste liquor ion composition (g / L) of this example was 24.5 sodium ions, 6.2 ammonium ions, 16.3 chlorine ions, 3.7 sulfate ions, 35.6 phosphate ions. 980 ml of water were evaporated in 1955 ml of initial waste liquor and 50% aqueous sodium hydroxide solution (42.0 ml) was added until the pH reached 9.04. The phosphate ion concentration of this solution was 68.4 g / L. Cooling proceeded to 15 ° C., disodium hydrogen phosphate 12-hydrate was obtained. The slurry was filtered through a centrifugal basket filter and washed with pure water.
[121]
The final product weighed 172.9 g. The ion composition (g / kg) of the final product was 112.8 sodium ions, 20.2 ammonium ions, 1.2 chlorine ions, 2.6 sulfate ions, 255.9 phosphate ions. The moisture content of the final product was 61.9 wt%. Total phosphoric acid recovery was 63.6 wt%.
[122]
Through this, it was found that the lower the phosphate ion concentration of the pH-adjusted concentrate, the lower the phosphoric acid recovery.
[123]
[124]
Example 2 Recovery of Phosphoric Acid According to Cooling Temperature
[125]
[126]
The O-phosphoserine fermentation based process waste liquor ion composition (g / L) of this example was 23.8 sodium ions, 6.5 ammonium ions, 15.1 chlorine ions, 3.4 sulfate ions, 35.0 phosphate ions. 1600 ml of water was evaporated (2000 ° C. or higher) in 2000 ml of initial waste liquor and 50% aqueous sodium hydroxide solution (39.5 ml) was added until the pH reached 9.00. The phosphate ion concentration of this solution was 159.4 g / L. This was an experimental condition similar to Example 1-2. Cooling proceeded to 25 ° C. and disodium hydrogen phosphate heptahydrate was obtained. The slurry was filtered through a centrifugal basket filter and washed with pure water.
[127]
The final product weighed 110.6 g. The ion composition (g / kg) of the final product was 126.8 sodium ions, 13.8 ammonium ions, 2.7 chlorine ions, 2.2 sulfate ions, 393.9 phosphate ions. The moisture content of the final product was 43.9 wt%. Total phosphoric acid recovery was 62.2 wt%. These results indicate that low temperatures are preferred for high phosphoric acid recovery.
[128]
[129]
Example 3. Phosphoric Acid Recovery with Further Adjustment of pH Before Concentration
[130]
[131]
The O-phosphoserine fermentation based process waste liquor ion composition (g / L) of this example was 23.4 sodium ions, 6.4 ammonium ions, 14.9 chlorine ions, 3.5 sulfate ions, 34.7 phosphate ions. Prior to the evaporation process, 50% aqueous sodium hydroxide solution (39.5 ml) was added until the pH reached 9.02. Thereafter, 1600 ml of water are evaporated from 2000 ml of initial waste liquor. Due to the evaporation of ammonia, the pH of the process solution after evaporation was 7.06. Therefore, 50% aqueous sodium hydroxide solution (16.0 ml) was added again until the pH reached 9.00. The phosphate ion concentration of this solution was 152.4 g / L. This concentration condition was similar to Examples 1-2. Cooling proceeded to 15 ° C., disodium hydrogen phosphate heptahydrate was obtained. The slurry was filtered through a centrifugal basket filter and washed with pure water.
[132]
The final product weighed 141.6 g. The ion composition (g / kg) of the final product was 156.8 sodium ions, 0.6 ammonium ions, 1.1 chlorine ions, 3.2 sulfate ions, 338.8 phosphate ions. The moisture content of the final product was 51.2 wt%. Total phosphoric acid recovery was 69.1 wt%. This method significantly reduced the content of ammonium ion impurities in the disodium hydrogen phosphate recovered than other processes.
[133]
[134]
Example 4 Recovery of Phosphoric Acid by Addition of Methanol
[135]
[136]
Example 4-1. Recovery of Phosphoric Acid by Addition of 100 ml Methanol
[137]
[138]
The O-phosphoserine fermentation based process waste liquor ion composition (g / L) of this example was 23.8 sodium ions, 6.4 ammonium ions, 15.3 chlorine ions, 6.4 sulfate ions, 37.1 phosphate ions. 1600 ml of water are evaporated from 2000 ml of initial waste liquor. Then 50% aqueous sodium hydroxide solution (48.0 ml) was added until pH reached 9.07 and 100 ml methanol was added. The phosphate ion concentration of this solution was 135.2 g / L. Cooling proceeded to 15 ° C., disodium hydrogen phosphate heptahydrate was obtained. The slurry was filtered through a centrifugal basket filter and washed with pure water.
[139]
The final product weighed 182.7 g. The ionic composition (g / kg) of the final product was 135.5 sodium ions, 24.9 ammonium ions, 13.1 chlorine ions, 2.2 sulfate ions, 328.5 phosphate ions. The moisture content of the final product was 49.6 wt%. Total phosphoric acid recovery was 81.0 wt%.
[140]
[141]
Example 4-2. Phosphoric acid recovery with 200 ml methanol addition
[142]
[143]
The O-phosphoserine fermentation based process waste liquor ion composition (g / L) of this example was 23.9 sodium ions, 6.4 ammonium ions, 15.3 chlorine ions, 3.9 sulfate ions, 37.3 phosphate ions. 1600 ml of water are evaporated from 2000 ml of initial waste liquor. Then 50% aqueous sodium hydroxide solution (47.0 ml) was added until the pH reached 9.04 and 200 ml methanol was added. The phosphate ion concentration of this solution was 115.2 g / L. Cooling proceeded to 15 ° C., disodium hydrogen phosphate heptahydrate was obtained. The slurry was filtered through a centrifugal basket filter and washed with pure water.
[144]
The final product weighed 182.0 g. The ion composition (g / kg) of the final product was 122.9 sodium ions, 38.9 ammonium ions, 15.9 chlorine ions, 2.4 sulfate ions, 328.2 phosphate ions. The moisture content of the final product was 46.6 wt%. Total phosphoric acid recovery was 80.2 wt%.
[145]
Through this, it was found that the addition of methanol in the pH adjustment step or after the pH adjustment increased the phosphoric acid recovery.
[146]
[147]
Example 5. Recovery of Phosphoric Acid by Two-Stage Crystallization
[148]
[149]
Based on the international publication WO 2014/182125, O-phosphoserine fermentation and L-cysteine ​​enzyme conversion reaction was performed. After this step, the ionic composition (g / L) of the process waste liquid was 21.7 sodium ions, 4.4 ammonium ions, 16.0 chlorine ions, 30.1 sulfate ions, 30.3 phosphate ions. 16.7 L of water was evaporated in 20 L of initial waste liquor and 50% aqueous sodium hydroxide solution (0.4 L) was added until the pH reached 9.00. The phosphate ion concentration of this solution was 162.1 g / L. Cooling proceeded to 15 ° C., disodium hydrogen phosphate heptahydrate was obtained. The disodium hydrogen phosphate heptahydrate slurry was filtered through a centrifugal basket filter and washed with pure water. The recovered disodium hydrogen phosphate heptahydrate weighed 1339.0 g. The ion composition (g / kg) of the material was 133.5 sodium ions, 18.7 ammonium ions, 5.3 chlorine ions, 1.3 sulfate ions, 323.4 phosphate ions. The moisture content of the final product was 48.2 wt%.
[150]
After slurry filtration, the ionic composition (g / L) of the filtrate was 13.3 sodium ions, 6.8 ammonium ions, 98.9 chlorine ions, 16.8 sulfate ions, 40.2 phosphate ions. To 3.2 L of the remaining filtrate, 1.1 g of disodium hydrogen phosphate 12-hydrate (sample prepared in Example 1-3) was added and stirred at 15 ° C. for 4 hours. The disodium hydrogen phosphate 12-hydrate slurry was filtered through a centrifugal basket filter and washed with pure water.
[151]
The recovered disodium hydrogen phosphate 12 hydrate weighed 299.0 g. The ion composition (g / kg) of the material was 128.2 sodium ions, 6.1 chlorine ions, 3.8 sulfate ions, 239.5 phosphate ions. Moisture content was 61.3 wt%. The final phosphate recovery of the above two step process was 83.2 wt. When the recovered disodium hydrogen phosphate heptahydrate and 12 hydrate were mixed and reused as a precursor of O-phosphoserine fermentation, the fermentation process proceeded without any problem.
[152]
[153]
Example 6 Preparation of O-Phososerine Using Recovered Disodium Phosphate
[154]
[155]
MMYE agar medium plate containing 50 μg / ml spectinomycin (2 g / L glucose, 2 mM magnesium sulfate, 0.1 mM calcium chloride, 6 g / L) using KCCM 11103P strain of WO 2012/053794 Incubated for 24 hours at 33 ° C. on sodium pyrophosphate, 0.5 g / L sodium chloride and 3 g / L disodium hydrogen phosphate, 10 g / L yeast extract, 18 g / L agar) Cells were scraped from one plate and 50 ml of flask seed medium (10 g / L glucose, 0.5 g / L magnesium sulfate, 3 g / L) containing 50 μg / ml spectinomycin in a baffle flask. Potassium dihydrogen phosphate, 10 g / L yeast extract, 0.5 g / L sodium chloride, 1.5 g / L ammonium chloride, 12.8 g / L sodium pyrophosphate, 1 g / L glycine) and inoculated at 30 rpm at 200 rpm. Seed incubation for 6 hours.
[156]
After completion of the seed culture, seed culture medium corresponding to 16% of the volume of the main culture medium was inoculated in a 1 L small fermentor filled with 300 ml of the main culture medium, and the culture was performed at pH 7.0 at 33 ° C. The culture was adjusted to pH 7.0 by adding ammonia water during incubation. After glucose was depleted in the medium, 520 g / L of aqueous glucose solution and 300 g / L of disodium hydrogen phosphate recovered herein were added to carry out fed-batch culture. After 80 hours of incubation, 29.3 g / L of O-phosphoseline was determined by HPLC.
[157]
[158]
Comparative example. Preparation of O-Phosphoserine Using Pure Phosphoric Acid
[159]
[160]
KCCM 11103P strains were cultured in the seed medium and the main medium in the same manner as in Example 6, and after the glucose was depleted, a fed-batch culture was performed by adding 520 g / L aqueous glucose solution and 200 g / L pure phosphoric acid. As a result of HPLC checking after 80 hours of incubation, 29.5 g / L of O-phosphoserine was measured.
[161]
[162]
In this way, the method of recovering and reusing the phosphoric acid of the present application from the fermentation medium can be recovered by crystallizing the phosphate from the fermentation broth or the fermentation broth, and if the recovered phosphate is reused for fermentation, the fermentation product in an amount similar to that of using pure phosphoric acid. As confirmed that it can be obtained, the method of the present application can be very useful in the fermentation process.
[163]
[164]
From the above description, those skilled in the art will appreciate that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. In this regard, the embodiments described above are to be understood in all respects as illustrative and not restrictive. The scope of the present application should be construed as including all changes or modifications derived from the meaning and scope of the following claims rather than the detailed description and equivalent concepts thereof.
Claim
[Claim 1]
(a) concentrating the fermentation broth or fermentation broth containing phosphoric acid (concentration step); (b) adjusting the pH of the concentrated concentrate to 8-11 (pH adjustment step); (c) crystallizing the phosphate from the pH-adjusted concentrate and separating it from the mother liquor (crystallization step); And (d) using the crystallized phosphate as a source of phosphoric acid in the fermentation medium (reuse step).
[Claim 2]
(A) in the fermentation medium containing phosphoric acid, using a microorganism to produce O-phosphoserine (O-phosphoserine, OPS) (OPS fermentation step); (b) O-phosphoserine sulfhydrylase (OPSS) or in the presence of a microorganism expressing the same, O-phosphoserine produced in step (a) by reacting with a sulfide sulfide fermentation solution of cysteine ​​or derivatives thereof Preparing a step (conversion step); (c) the fermentation broth; Or concentrating the fermentation waste liquid from which the cysteine ​​or derivative thereof is removed from the fermentation broth (concentration step); (d) adjusting the pH of the concentrated concentrate to 8-11 (pH adjustment step); (e) crystallizing the phosphate from the pH adjusted concentrate (separation step) from the mother liquor; And (f) using the crystallized phosphate as a source of phosphoric acid in the fermentation medium (reuse step).
[Claim 3]
(a) producing O-phosphohomoserine (OPHS) using a microorganism in a fermentation medium containing phosphoric acid (OPHS fermentation step); (b) O-phosphohomoserine dependent methionine synthase or O-phosphohomoserine dependent methionine synthase or in the presence of a microorganism expressing the same, the O-phosphohomoserine produced in step (a) by reacting with sulfide to methionine Or preparing a fermentation broth of a derivative thereof (conversion step); (c) the fermentation broth; Or concentrating the fermentation waste liquid from which methionine or its derivatives are removed from the fermentation broth (concentration step); (d) adjusting the pH of the concentrated concentrate to 8-11 (pH adjustment step); (e) crystallizing the phosphate from the pH adjusted concentrate (separation step) from the mother liquor; And (f) using the crystallized phosphate as a source of phosphoric acid in the fermentation medium (reuse step).
[Claim 4]
(a) concentrating the fermentation waste liquor containing phosphoric acid (concentration step); (b) adjusting the concentrated concentrate to pH 8-11 (pH adjustment step); (c) crystallizing the phosphate from the pH adjusted concentrate (crystallization step); And (d) separating the crystallized phosphate from the mother liquor (separating step).
[Claim 5]
The fermentation broth of claim 1, wherein the fermentation broth comprises (i) an O-phosphoserine (OPS) fermentation broth, (ii) an O-phosphohomoserine (OPHS) fermentation broth, or (iii) the fermentation broth. Based on a converting enzyme or microorganism expressing it; And a fermentation broth comprising an amino acid or a derivative thereof prepared by adding sulfide, and the fermentation broth is a waste liquid from which the amino acid or derivative thereof is removed from the fermentation broth.
[Claim 6]
The method of claim 2 or 3, wherein the sulfide is at least one selected from the group consisting of CH 3 SH, Na 2 S, NaSH, (NH 4 ) 2 S, H 2 S and Na 2 S 2 O 3 . .
[Claim 7]
The method according to claim 1 or 4, wherein the fermentation waste liquid is a waste liquid from which amino acids or derivatives thereof are removed from the fermentation broth.
[Claim 8]
The method of any one of claims 1 to 4, wherein the pH adjusting step is performed by adding hydroxide to the concentrate.
[Claim 9]
The method of claim 8, wherein the hydroxide is sodium hydroxide or aqueous sodium hydroxide solution.
[Claim 10]
The method of any one of claims 1 to 4, further comprising cooling the pH adjusted concentrate between the pH adjustment step and the crystallization step or in the crystallization step.
[Claim 11]
5. The method of claim 1, further comprising adding phosphate crystals to the nucleus between the pH adjustment step and the crystallization step or between the crystallization steps. 6.
[Claim 12]
The method of any one of claims 1 to 4, wherein the phosphate salt comprises one or more selected from the group consisting of trisodium phosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate and hydrates thereof.
[Claim 13]
The method of claim 12, wherein the hydrate is disodium hydrogen phosphate heptahydrate or disodium hydrogen phosphate hexahydrate.
[Claim 14]
The method of claim 1, further comprising crystallizing the phosphate from the mother liquor (secondary crystallization step).
[Claim 15]
The method of claim 14, wherein the secondary crystallization step comprises: (i) concentrating the mother liquor (reconcentrating step), (ii) adjusting the pH of the mother liquor to 8 to 11 (pH readjustment step), and ( iii) crystallizing and separating the phosphate from the pH adjusted mother liquor (recrystallization step).
[Claim 16]
The method of claim 15, further comprising adding phosphate crystals as nuclei to the mother liquor between the pH readjustment step and the recrystallization step.
[Claim 17]
The method of any one of claims 1 to 4, further comprising adjusting the fermentation broth or fermentation broth to pH 8-11, prior to the concentration step.
[Claim 18]
The method of claim 1, further comprising: adjusting the pH; Or adding alcohol between the pH adjustment step and the crystallization step.
[Claim 19]
The method of claim 18, wherein the alcohol is methanol, ethanol, propanol, butanol or a combination thereof.