Description:The present invention of the for a method for the use of Bacillus clausii in animal feed for animals namely poultry, swine and canine. Bacillus clausii is mixed in an amount of 2.5 billion CFU per gram and doses range 50-250 gm per ton of feed. This specific mixture is then fed to the animals such as poultry, swine and canine. This use of Bacillus clausii in poultry increases weight gain and/or improves the Feed Conversion Ratio (FCR) in broiler. Such use of Bacillus clausii also reduces diarrhea problem in above-mentioned animals. For determining the efficacy of Bacillus clausii, several trials were conducted, the results of which are as follows:

The practices were conducted in commercial broiler and layer farms.

In broilers: In broiler farms, four trials were conducted with different dose and concentration of Bacillus clausii.

In one trial day old chickens were fed specific diet as control group (T1) feed without Bacillus clausii and another group (T2) was reformulate with Bacillus clausii @ 250 gm dose per ton of feed than the control group. Diet and their specification shown in Table 1 and 2. The Bacillus clausii brand was obtained from DSAND group India. All manage mental conditions were remain same for both group. At the end of trial result concluded in Table 3. Result indicates significantly better profitability in Bacillus clausii group T2.

Table 1: Composition of Feed and their Specification

WITHOUT BACILLUS CLAUSII
Ingredients Prestarter Starter Finisher
Maize 567.99 574.81 613.05
Soybean meal 340.33 317.74 267.81
Calcite/LSP 6.12 6.06 6.00
Di calcium phosphate 3.53 3.89 4.56
Meat & Bone meal 50.00 50.00 50.00
Oil 19.27 35.04 46.29
Methionine 1.77 2.05 2.09
Lysine 0.89 0.42 0.00
SodaBicarb 1.00 1.00 1.00
Salt 2.50 2.50 2.50
Trace Min 1.25 1.25 1.25
Vit. Premix 0.60 0.50 0.50
Bacillus clausii 0.00 0.00 0.00
Acidifier 1.00 1.00 1.00
Antioxidant 0.10 0.10 0.10
Herbal Choline 1.00 1.00 1.20
Livtonic 0.40 0.40 0.40
Betain 0.50 0.50 0.50
Emulsifier 0.50 0.50 0.50
Toxin Binder 1.00 1.00 1.00
Herbal Anti Diarroeal 0.25 0.25 0.25
TOTAL 1,000.00 1,000.00 1,000.00
Analysis Specifications
Prestarter Starter Finisher
C.Protein 23.00 22.00 20.00
ME Mcal/Kg 3.00 3.10 3.20
Calori protein ratio 130.43 140.91 160.00
C.Fibre 3.75 3.63 3.45
EE + fat % 4.43 5.98 7.14
Amino Acids
Lysine 1.30 1.20 1.03
Methionine 0.53 0.55 0.52
Threonine 0.95 0.90 0.81
Minerals
Calcium 1.00 1.00 1.00
Available Phosphorus 0.45 0.45 0.45

Table 2: Composition of Feed and their Specification

WITH BACILLUS CLAUSII
Ingredients Pre-Starter Starter Finisher
Maize 608.42 615.24 653.14
Soybean meal 313.70 291.10 241.78
Calcite/LSP 6.15 6.09 6.04
Di calcium phosphate 3.78 4.14 4.80
Meat & Bone meal 50.00 50.00 50.00
Oil 4.93 20.70 32.05
Methionine 1.44 1.72 1.75
Lysine 1.23 0.75 0.00
SodaBicarb 1.00 1.00 1.00
Salt 2.50 2.50 2.50
Trace Min 1.25 1.25 1.25
Vit. Premix 0.60 0.50 0.50
Bacillus clausii 0.25 0.25 0.25
Acidifier 1.00 1.00 1.00
Antioxidant 0.10 0.10 0.10
Herbal Choline 1.00 1.00 1.20
Livtonic 0.40 0.40 0.40
Betain 0.50 0.50 0.50
Emulsifier 0.50 0.50 0.50
Toxin Binder 1.00 1.00 1.00
Herbal Anti Diarroeal 0.25 0.25 0.25
TOTAL 1,000.00 1,000.00 1,000.00
Analysis Specifications
Prestarter Starter Finisher
C.Protein 22.13 21.13 19.13
ME Mcal/Kg 2.95 3.05 3.15
Calori protein ratio 133.34 144.39 164.72
C.Fibre 3.71 3.59 3.41
EE + fat % 3.12 4.67 5.84
Amino Acids
Lysine 1.26 1.16 0.97
Methionine 0.49 0.50 0.48
Threonine 0.91 0.86 0.77
Minerals
Calcium 1.00 1.00 1.00
Available Phosphorus 0.45 0.45 0.45

Table 3: Performance of Broiler Chicken

GROUP Duration (Days) Avg. Body Wt. FCR Feed Consumption COST OF FEED (?) Cost of Production (?) Net Profit per bird (?)*
T1 35 2.09 1.67 3.49 88.10 114.10 11.30
T2 34 2.13 1.65 3.51 86.28 112.28 15.52

Conclusion:

Based on the results of the preceding trials, it has been determined that the application of Bacillus clausii at various doses and concentrations improves FCR and economics in broilers. Finally, Bacillus clausii is a probiotic bacteria that has been found to have several benefits in poultry. It can aid in the promotion of a healthy intestinal environment, the reduction of stress, the improvement of feed conversion, and the reduction of the need for antibiotics. These advantages can lead to enhanced general health and performance of poultry, resulting in increased production and profitability for poultry producers.

To guarantee its efficiency, Bacillus clausii should be used under the supervision of a veterinarian or poultry specialist, and suitable storage and application techniques should be followed. Furthermore, before utilising Bacillus clausii in poultry production, it is important to be aware with the rules and regulations governing the use of probiotics in animals.

Economic Significance and Technical Advancement

• Use of Bacillus clausii in poultry increases weight gain and/or improves the Feed Conversion Ratio (FCR) in broiler.
• Use of Bacillus clausii and its use in poultry reduces diarroea problem.

While much emphasis has been put herein on the specific aspects of this invention, it will be realised that different modifications can be made, and that many changes in the preferred embodiments can be made without straying from the concept of the invention.

Throughout this specification the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or group of elements, integers or steps.

The use of the expression "at least" or "at least one" suggests the use of one or more elements or ingredients or quantities, as the use may be in the embodiment of the invention to achieve one or more of the desired objects or results.

"Whenever a range of values is specified, a value up to 10% below and above the lowest and highest numerical value respectively, of the specified range, is included in the scope of the invention". , Claims:I claim the following:

1) A method for the use of Bacillus Clausii in animal feed (for poultry, swine and canines), comprising of:

• Mixing of Bacillus clausii in an amount of 2.5 billion CFU per gram and doses range 50-250 gm per ton of feed;
• Feeding the mixed feed to animals to the poultry and also in drinking water.

2) Method for the use of Bacillus Clausii in animal feed, as claimed in claim (1), wherein Bacillus Clausii is effective in reducing the pathogenic dysbiosis in poultry gut.

3) Method for the use of Bacillus Clausii in animal feed, as claimed in claim (1), wherein Bacillus Clausii is helpful in increasing weight gain and improving the Feed Conversion Ratio (FCR) and reducing feed cost of the poultry.

4) Method for the use of Bacillus Clausii in animal feed, as claimed in claim (1), wherein Bacillus Clausii is used in animal feed which includes broiler, layer and breeders.