Blood culture bottles (BCB) contains the aerobic and anaerobic culture medium and the charge resins used
for the culturing of highly pathogenic microbial cells for the detection of infection causing deadly
bacteremia. These bottles are highly recommended in hospitals and pathology laboratories for pathogens
detection. The benefit of using these culture bottles is the Low risk of secondary contamination due to
direct inoculation. Blood culture bottles (BCB) media provide a unique & superior formulation to enhance
recovery of aerobic and anaerobic bacteria, fungi, and yeast. The cationic resins used in Blood culture
bottles (BCB) neutralize most antimicrobial and preservative components present in product formulation.
As per GSR 343 (E) March 28, 2016 it is mandatory to pre-treat the laboratory waste, microbiological waste,
blood samples,and blood bags through disinfection or sterilization methods on-site of generation before
being transported for tertiary disposal as prescribed by the World Health Organization (WHO) or National
AIDs Control Organization (NACO). According to this guideline of these agencies no one not directly release
the Blood culture bottles (BCB) without disinfection/ sterilization.
The rubber septum of the blood culture bottle must be disinfected wr7i0t%h ec thanol prior to inoculation. For
sterilization and disinfection, the legacy technology was used such as an toclave, ETO etc. these all have
huge limitations
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PROVISIONAL The following specification particularly
The following specification describes the
invention
1
describes the invention and the manner in
which is to be performed
4. DESCRIPTION (Description shall start from next stage)
Description
The microwave is one of the recommended procedures for this pretreatment at the point of
generation. The sectors that under the preview of biomedical waste includes hospitals, nursing homes,
clinics, dispensaries, veterinary institutions, animal houses, pathological laboratories, blood banks,
AYUSH hospitals, clinical establishments, research or educational institutions, health camps, medical or
surgical camps, vaccination camps, blood donation camps, first aid rooms of schools, forensic
laboratories and research labs. Thus, the present invention has great commercial value.
Due to microwave exposure,lateron, a new problem also arises that is the microwaves are nonionic
electromagnetic waves which are synchronizedoscillations of electric and magnetic fields. Thus there is
a strong interaction betweenmicrowaves and charged atoms. SinceBiood culture bottles (BCB) bottles
contain charged resins and thusshows strong interaction with microwaves leading to excessive heating.
If directly exposed tomicrowaves Blood culture bottles (BCB) bottles may explode. However, there are
snags in the existing recommended procedures:
As of Now, there is no specific method for Blood culture bottles (BCB) disinfection/sterilization,
therefore, Disinfection/sterilization method proposed here has no competition as of now.
So to solve the problem Disinfection/ Sterilization and explosion of Blood culture bottles (BCB) a
process of microwave treatment is to optimize in such a way that there is no microbial contamination
present during the disinfection/ sterilization cycle plus no explosion of bottles occurs during pretreatment
process at respective places.
Brief of invention
The present invention is a Microwave based method for the disinfection/sterilization and to prevent
the explosion of Blood culture bottles (BCB).There is an urgent need to develop appropriate and
alternative technology for Blood culture bottles (BCB)sterilization and disinfection/ decontamination
for the safer use and disposal. Microwave being green and low-cost technology provides an efficient
and quick solution to the Blood culture bottles (BCB)disinfection I sterilization process. It has been
shown in previous studies that Microwaves have effective microbicidal and sporicidal properties. The
traditional method of conductive heating using steam and hot-air, or even radiation heating, is a slow
and inefficient method for transferring energy into the system since it depends on convection currents
and on the thermal conductivity of the various materials that must be penetrated. This generally
results in the temperature of the vessel being higher than that of the stuffings. In contrast, Microwave
chemistry is based on the efficient heating of materials by microwave dielectric heating effects.
Field of the invention,
Microwave dielectric heating depends on the ability of a specific material to absorb microwave energy
and convert it into heat. Since, Microwaves are electromagnetic waves that consist of an electric and
magnetic field component, substances such as water which possess a dipole moment to generate heat
when irradiated with microwaves. When exposed to microwave frequencies, the dipoles of the water
align with the applied electric field. As the field oscillates, the dipole field attempts to realign itself with
the alternating electric field and, in the process, energy is lost in the form of heat through molecular
friction and dielectric loss. Thus, microwave irradiation produces efficient internal heating (in core
volumetric heating) by direct coupling of microwave energy with the molecules within the microbes or
the medium harboring them. The present invention is designed to work in Microwave equipment with
water sprinkling mechanism. Therefore, Microwaves penetrates the Blood culture bottles (BCB)
uniformly and generate heat directly in the inner core of the Blood culture bottles (BCB) piles. The
present method of the invention uses a Microwave based disinfection device with a water sprinkling
system and a single magnetron (OptiMaser, l.SkW, 30 lit) for disinfection/ sterilization of Blood culture
bottles (BCB).
2
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Background of the Invention
At the start of disinfection cycle Blood, culture bottles (BCB) containing bags were sprinkled with water
and followed by exposure of 2.45 GHz Microwaves (loo·c, 10 min). The average time is taken for the
Blood culture bottles (BCB) disinfection/sterilization cycle in OptiMaser™ (Microwave based
disinfection device) was observed to be 45 min. This exposure did not cause any changes in physical
properties in the Blood culture bottles (BCB). And to solve the second problem of BCB there is a barrier
is placed to moderate the Microwave exposure to Blood culture bottles (BCB)than explosion could be
prevented. The barrier used to reduce the intensity of microwave a double layered moist cotton fiber
cloth to cover the whole blood culture bottles (BCB). A disinfection efficacy of 10 log reduction was
achieved for bacteria, fungi, and heat resistant bacterial spores. A self-contained biological indicator
having 106B. atrophaeus spores was used for monitoring the disinfection process.
A patent search revealed that microwave-based disinfection/sterilization of Blood culture bottles (BCB)
as mentioned in the present invention is not claimed elsewhere.
The databases searched were
-Google Patent Search
- IP Patent search
- USPTO
- Patentscope
OBJECTIVES OF INVENTION
• The main objective is to develop a method for the disinfection of infected Blood culture
bottles (BCB).
• Second one objective is to develop a microwave-based method for the sterilization of Blood
culture bottles (BCB) items used in the Academic institutions, R&D laboratories, and
hospitals.
And the third one and very important is to develop or find a barrier to solve the problem of the
explosion of Blood culture bottles (BCB).

CLAIMS (Not applicable for provisional specification. Claims should start with the preamble
- "1/We claim" on separate page)
What is claimed is:
1. A device for disinfecting of Blood culture bottles (BCB) and thereof., comprising:
a) Blood culture bottles (BCB)are fed into the Microwave bin lined with microwave
compatible plastic polybag.
b) As the first step of disinfection cycle water is sprinkled into the bin to moisten the Blood
culture bottles (BCB) polybag.
c) A 10 min exposure at 100°(, 10 min hold time is sufficient to neutralize the Bacterial cells
(Gram positive and Gram negative).
d) A 10 min exposure at 100°C, 10 min hold time is sufficient to neutralize the Fungi and
Yeast cells.
e) A 10 min exposure at 100°C, 10 min hold time is sufficient to neutralize the heat-resistant
sporesof both bacterial and fungal.
2. A microwave-based method for sterilization of Blood culture bottles (BCB) :Blood culture bottles
(BCBcould be fed into a microwave compatible plastic bag with a zip lock. Blood culture bottles
(BCBbags could be arranged in the bin rack and exposed to microwaves in standardized Blood culture
bottles (BCB) disinfection cycle.
3. A microwave-based method for prevention of Blood culture bottles (BCB) from microwave intensity
explosion: Blood culture bottles (BCB could be fed into a microwave compatible plastic bag with a zip
lock. Blood culture bottles (BCB) bags could be arranged in the bin rack and covered with double
layered moist cotton cloth fabric as a barrier to reduce the intensity after exposed to microwaves in
standardized disinfection/ sterilization cycle.
The method to claim, claims 1 and 2 wherein the microwaves used has a frequency of 2.45GHz. The
method to claim, claims 3wherein the Blood culture bottles (BCB) material containing the charge resins
highly attract the microwave and blast after exposure.