Claims:WE CLAIM:
1. A synergistic composition for attracting mosquitoes, comprising Butylated Hydroxy Toluene ranging from about 0.1% w/w to about2% w/w, Isopropanol ranging from about 0.1%w/w to about 2% w/w, dissipating agent ranging from about 94%w/w to 98%w/w and perfume ranging from about 0.1% w/w to 2%w/w
2. The synergistic composition as claimed in claim 1, wherein Butylated Hydroxy Toluene is 1 % w/w, Isopropanol is 1% w/w, dissipating agent is 97% w/w and perfume is 1% w/w.
3. The synergistic composition as claimed in claim 1 and Claim 2, wherein the perfume is selected from natural fragrance.
4. The synergistic composition as claimed in claim 1 and Claim 2, wherein the dissipating agent is selected from a group comprising Deodorized kerosene and ionized water.
5. The synergistic composition as claimed in claim 3, wherein the perfume is Sandal R fragrance comprising hexahydro-4,6,6,7,8,8-hexamethyl cyclopenta-2-benzopyran , and 1,2,3,4,5,6,7,8-octahydro-2,3,8,8-tetramethyl acetonaphthone.
6. A process for preparation of synergistic composition for attracting mosquitoes comprising Butylated Hydroxy Toluene ranging from about 0.1% w/w to about2% w/w , Isopropanol ranging from about 0.1%w/w to about 2% w/w, dissipating agent ranging from about 94%w/w to 98%w/w and perfume ranging from about 0.1% w/w to 2%w/w, said process comprising acts of
i) mixing Butylated Hydroxy Toluene, and isopropanol and stirring to obtain a homogenous solution;
ii) adding dissipating agent to the homogenous solution to obtain a mixture; and

iii) adding perfume to the mixture and stirring to obtain the synergistic composition for attracting mosquitoes comprising Butylated Hydroxy Toluene ranging from about 0.1% w/w to about2% w/w , Isopropanol ranging from about 0.1%w/w to about 2% w/w, dissipating agent ranging from about 94%w/w to 98%w/w and perfume ranging from about 0.1% w/w to 2%w/w.
7. The process as claimed in claim5, wherein Butylated Hydroxy Toluene, and isopropanaol are mixed in 1:1 ratio and stirred for a period ranging from about 4 minutes to about 6minutes preferably 5 minutes at a speed ranging from about25 rpm to about 35 rpm preferably 30rpm.
8. The process as claimed in claim 5 wherein the perfume is added to the homogenous mixture and stirred for a period ranging from about 1 to about 3 minutes preferably one minute at a speed ranging from about 120 rpm to about 130 rpm preferably125 rpm.
9. The process as claimed in claim 5, wherein the preparation of the composition is carried out at a temperature ranges from about 35°C to about 45°C.

10. A method of attracting the mosquitoes, said method comprising act of coating a surface or a device containing composition comprising Butylated Hydroxy Toluene ranging from about 0.1% w/w to about2% w/w, Isopropanol ranging from about 0.1%w/w to about 2% w/w, dissipating agent ranging from about 94%w/w to 98%w/w and perfume ranging from about 0.1% w/w to 2%w/w and attracting mosquitoes, preferably female mosquitoes.
, Description:FIELD OF INVENTION
The present invention relates to the field of insect attractants. More specifically, the invention relates to a synergistic composition for attracting mosquitoes.In particular, the invention relates to a synergistic composition comprising ButylatedHydroxy Toluene (BHT) Isopropanol, dissipating agent, and perfume, for attracting female mosquitoes. The invention also provides a process for the preparation of the mosquito attractant composition.
BACKGROUND OF INVENTION
Mosquito belonging to the family Culicidae are the most common pests which transmit various forms of diseases including malaria, chikangunya, dengue, Zika and the like, affecting human health as well as killing millions of people all over the world. These insects feed on the blood of various kinds of hosts including man and act as vectors of diseases. They bite infected humans and pick up the virus along with their blood-meal. Specifically, the female mosquitoes pick up the parasite from infected people when they bite to obtain blood to nurture their eggs. The mosquitoes then bite other humans and transmit the virus to them. There is a need for a strategy to eradicate these disease causing organisms by using safe, environment friendly, benign products.Though a number of products to eradicate mosquitoes are available in the market, most of them being pesticides or insecticides cause harmful side effects both to humans as well as to the environment.
Mosquitoes are usually drawn to humans through human body scent. The mosquito has a scent tracking ability which can recognise sweat, dead cells,temperature of the body and also exhaled carbon dioxide. Attempts are being made to design attractants to mimic the scent profile of humans in order to attract, capture and destroy these pests.
US5799436 describes an apparatus which utilizes an attractant composition to attract mosquitoes and flies. The composition used in the device is isoctenol (1-octen-3-ol) used in combination with heat and carbon dioxide. The said invention however has a limited mosquito attracting capacity and hence is not effective in heavily mosquito infested zones.
US 8734773 describes a method which comprises isobutyric acid, isovaleric acid, octanal, nonanal, decanal, dodecanal, tridecanal, 2-methylphenol, and a carrier for preparing an insect attractant composition, by selecting at least two compounds found in composition are associated with human odours. The aforementioned method of preparing the insect attractant composition is elaborate and time consuming and also expensive.
US 20070157508 describes a method which comprises steps of preparing a mosquito bait composition which emits an odour similar to that produced from human body wherein the mosquito bait composition is prepared from lactic acid, amino acid, sodium chloride, glycerin, acylglycerol, triacylglycerol, lipid acid, stearic acid, lipoprotein, cholesterol, cholesterol ester, steroid and wax..
The aforementioned process of preparation of the attractant is tedious and time consuming also the availability of the ingredients as well as the cost associated with the process are not facile.
Hence there is a need for an effective remedy for bringing about maximum attraction of mosquitoes and also a solution for attracting mosquitoes which is safe, independent of toxic components, andenvironment friendly.
SUMMARY OF INVENTION
Accordingly, the present invention is in relation to a synergistic composition for attracting mosquitoes, in order to capture and/or kill the harmful pests. The synergistic composition comprises Butylated Hydroxy Toluene, Isopropanol, dissipating agent and perfume. The invention also provides for a process for the preparation of the composition which comprises mixing of isopropanol and Butylated Hydroxy Toluene in 1:1 proportion and then adding dissipating agent to it. Perfume is then added to the mixtureof the composition to obtain the synergistic composition. The synergistic composition thus obtainedattracts more than 80% mosquitoes,preferably female mosquitoes, from the surrounding area in which the said composition has been exposed. Also, the composition offers no side effects and can be used without any limitations in the presence of children, elderly persons or sick people.
DETAILED DESCRIPTION OF INVENTION
The foregoing description of the embodiments of the invention has been presented for the purpose of illustration. It is not intended to be exhaustive or to limit the invention to the precise form disclosed as many modifications and variations are possible in light of this disclosure for a person skilled in the art in view of the description and claims. Unless defined otherwise, all technical and scientific terms used herein have the same meanings as commonly understood by person skilled in the art.
The present invention is in relation to a synergistic composition for attracting mosquitoes, comprising Butylated Hydroxy Toluene ranging from about 0.1% w/w to about2% w/w , Isopropanol ranging from about 0.1%w/w to about 2% w/w, dissipating agent ranging from about 94%w/w to 98%w/w andperfumeranging from about 0.1% w/w to 2%w/w.
In an embodiment of the invention the synergistic composition for attracting mosquitoes comprises 1% w/wButylated Hydroxy Toluene , 1 % w/w Isopropanol, 97% w/w dissipating agentand1% w/w perfume.
In another embodiment of the invention, the synergistic composition , wherein the perfume is selected from natural fragrance.
In another embodiment of the invention, the synergistic composition, wherein the dissipating agent is selected from group comprising Deodorized Kerosene and ionised water.
In yet another embodiment of the invention, the synergistic composition wherein the perfume is Sandal R fragrance comprising hexahydro-4,6,6,7,8,8-hexamethyl cyclopenta-2-benzopyran , and 1,2,3,4,5,6,7,8-octahydro-2,3,8,8-tetramethyl acetonaphthone.
The present invention is also in relation to aprocess for preparation of synergistic composition for attracting mosquitoes comprising,Butylated Hydroxy Toluene ranging from about 0.1% w/w to about2% w/w , Isopropanol ranging from about 0.1%w/w to about 2% w/w, dissipating agent ranging from about 94%w/w to 98%w/w and perfume ranging from about 0.1% w/w to 2%w/w said process comprising acts of
i) mixing Butylated Hydroxy Toluene, and Isopropanol and stirring to obtain a homogenous solution;
ii) adding dissipating agent to the homogenous solution to obtain a mixture; and
iii) adding perfume to the mixture and stirring to obtain the synergistic composition for attracting mosquitoes comprising Butylated Hydroxy Toluene ranging from about 0.1% w/w to about2% w/w , Isopropanol ranging from about 0.1%w/w to about 2% w/w, dissipating agent ranging from about 94%w/w to 98%w/w and perfume ranging from about 0.1% w/w to 2%w/w.
In an embodiment of the invention the process, wherein Butylated Hydroxy Toluene, and Isopropanol are mixed in 1:1 ratio and stirred for a period ranging from about 4 minutes to about 6minutes preferably 5 minutes at a speed ranging from about25 rpm to about 35 rpm preferably 30rpm.
In another embodiment of the invention, the process wherein the perfume is added to the homogenous mixture and stirred for a period ranging from about 1 to about 3 minutes preferably one minute at a speed ranging from about 120 rpm to about 130 rpm preferably125 rpm.
In yet another embodiment of the invention the process, wherein the preparation of the composition is carried out at a temperature ranges from about 35°C to about 45°C.
The present invention is also in relation to a process of attracting mosquitoes, theprocess comprising act of coating a surface or a device containing composition comprising about 0.1% to 2% w/w Butylated Hydroxy Toluene, about 0.1% to 2% w/w Isopropanol, about 94% to 98%w/w dissipating agent and about 0.1% to 2% w/w perfume and attracting mosquitoes preferably female mosquitoes.
The present invention is in relation to a synergistic composition for attracting mosquitoes, in particular female aedes mosquito, for capturing and /or killing the pest. Mosquito are generally attracted to humans through their body odour either through sweat, dead cells, and other factors including exhaled Carbon dioxide and body heat from a human being. The attractant composition of the present invention mimics the human odour which is sensed by the mosquitoes at a distance and get attracted towards the source.
Also, the mosquitoes look for warm blooded animals who have blood with high protein content. When a device comprising a composition which emanates odour that mimics the blood with high protein content, the mosquitoes are drawn towards the device rather than any human specimen standing beside the device. Similarly, the synergistic composition of the present invention attracts mosquito, and draws the mosquitoes towards the emanating source which can then be trapped.
The invention also describes a process for the preparation of the synergistic composition which comprises mixing Butylated Hydroxy Toluene(BHT) and Isopropanol (IP). The solution thus obtained is stirred until the BHT is completely dissolved in Isopropanol. Dissipating agent selected from a group of Deodorized kerosene and ionized water is then added to the BHT-IP solution. Perfume is added to the BHT-IP-DOK solution and stirred The perfume is selected from a range of natural fragrances. The perfume used in this invention is sandalwood(R) which is a composition ofhexahydro-4,6,6,7,8,8-hexamethyl cyclopenta--2-benzopyran and1,2,3,4,5,6,7,8-octahydro-2,3,8,8-tetramethyl acetonaphthone.
Various combinations of the composition are tested for understanding the efficacy of the composition. Individually the BHT, IP, DOK or the perfume do not possess attractant property. However, the components in a specific combination or ratio, function as an attractant.
The combinations with varying proportions of the constituents in the composition to test the synergy wherein the dissipating agent is deodorized kerosene are provided below.
Sl.No BHT w/w IP w/w DOK w/w Perfume w/w Success of mosquito attracting %
1 10% 10% 80 % 0 % 0%
2 .1 % .1 % 94.8% 5 % 5%
3 1% 1% 97% 1% 80%
4 0% 1% 98% 1% 0%
5 1% 0% 98% 1% 0%

The composition with 1%w/w Butylated Hydroxy Toluene, 1% w/w Isopropanol, 97% w/w deodorised Kerosene and 1% w/w perfume is identified to be producing the best result as compared to other compositions and hence used for experimental purpose as explained below:
EXPERIMENTAL
Example 1:
Two chambers are set besides each other wherein the two chambers are connected through an interconnecting opening. The first chamber comprises mosquitoes while second chamber comprises a human specimen and a device containing the mosquito attractant composition. As the odour from the attractant composition is released from the device the mosquitoes present in the first chamber are drawn towards the device placed in the second chamber. Around 80% of the mosquitoes present in the first chamber are attracted to the mosquito attractant composition placed in the device.
Example 2:
Preparation of the synergistic composition for attracting mosquitoes:
At a temperature ranging from about 35°C to about 45°C, 1% w/w Butylated Hydoxy Toluene (BHT) is taken in a container. 1%w/w isopropanol (IP) is then added to BHT. The two components on mixing in 1:1 proportion are stirred for about 5 minutes at 30 rpm or until the BHT completely dissolves in IP to obtain BHT-IP mixture. 97% w/w of dissipating agent is added to the BHT -IP mixture. 1% w/w perfume is then added to the BHT-IP-dissipating agent mixture. The mixture is stirred for about a minute at 125rpm. The composition thus obtained is used as a mosquito attractant.
Other combinations were prepared similar to example 1 as given in Table 2
Table-2: Examples of different combinations of the composition of present invention using DOK as the dissipating agent
Sl No Isopropanol (w/w) ButylatedHydroxy Toluene(w/w) Deodorized Kerosene(w/w) Perfume (w/w)
Example 2 0.9% w/w 0.8%w/w 97.3% w/w 1%
Example 3 1% 0.9% 97.1% 1%
Example 4 0.9 1% 97.1% 1%
Example 5 1% 1% 97.25 .75%

Table 3: Examples of different combinations of the composition of present invention using ionised water as the dissipating agent
Sl No Isopropanol (w/w) ButylatedHydroxy Toluene(w/w) Ionised water(w/w) Perfume (w/w)
Example 5 1% 1% 97% 1%
Example 6 1% .9% 97.1% 1%
Example 7 .9% 1% 97.1% 1%
Example 8 1% 1% 97.25 .75%

Thus the present invention provides an economical environmental friendly synergistic composition for use as a mosquito attractant. The invention also discloses a simpleprocess for the preparation of the mosquito attractant composition involving eco-friendly reagents.
The aforesaid description is enabled to capture the nature of the invention. It is to be noted however that the aforesaid description and the appended figures illustrate only a typical embodiment of the invention and therefore not to be considered limiting of its scope for the invention may admit other equally effective embodiments.
It is an object of the appended claims to cover all such variations and modifications as can come within the true spirit and scope of the invention