DESC:FIELD OF THE INVENTION
[0001] The present disclosure generally relates to a disinfectant composition. Specifically, the present invention relates to a multipurpose biodegradable disinfectant composition and disinfectant hand sanitizer comprising amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid, and one or more pharmaceutically or cosmetically acceptable excipients. Both the disinfectant compositions of the present disclosure possess excellent long lasting antimicrobial, antifungal and virucidal property.

BACKGROUND OF THE INVENTION
[0002] Background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.
[0003] Antiseptics and disinfectants were used extensively in hospitals and other health care settings for a variety of topical and hard-surface applications. In particular, they are an essential part of infection control practices and aid in prevention of nosocomial infections. However, after onset of covid-19 pandemic disinfectants have become integral part of human life.
[0004] Mounting concerns over potential microbial contamination and infection risks of general health, the consumer markets have also flooded with products of antiseptics and disinfectants category by general public for household as well as personal commercial operations, including restaurants, nursing homes, hospitals, and laboratories.
[0005] A wide variety of chemicals agents are used in disinfectants, many of which have been used for hundreds of years, including alcohols, phenols, iodine, and chlorine. Most of these active agents demonstrate broad-spectrum of anti-microbial activity and they are used to reduce microbes on living tissue or medical equipment and other inmate objects. However excessive uses of these products have prompted some speculation on development of microbial resistance in microbes. The mechanism of resistance to antiseptic and disinfectant solutions includes cellular impermeability, biofilm formation, efflux, and mutations at the target site or over expression at the target site.
[0006] Despite best efforts to eliminate these infectious micro-organisms, they continue to emerge and re-emerge, contributing to human illness and death, especially as a result of infections acquired in the hospital. The successful eradication of these pathogens with antiseptic/disinfectant solutions have been complicated by development of highly resistant strains. It has been also reported that disinfectant designed for control of micro-organisms are subjected to microbial contamination.
[0007] US20120321582A1 discloses an aqueous and biodegradable air sanitizer formulation comprising Active – Amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid (less than 10%) as antimicrobial compound.
[0008] EP3542628A1 discloses a disinfectant composition which uses Active – Amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid as surfactant.
[0009] Various disinfectant composition and methods of preparation of disinfectant composition are available in the art. However, the compositions available in the market have serious drawbacks like alcohol-based, shorter protection, frequent use of the disinfectant, unstable, and hence, efficacy drastically goes down as the time progresses.
[0010] There is, therefore, an unmet need to develop a disinfect composition or hand sanitizer which is biodegradable, stable, safe, effective, alcohol-free and has long lasting antimicrobial protection.

OBJECTS OF THE INVENTION
[0011] An object of the present invention is to provide a multipurpose biodegradable disinfectant or hand sanitizer composition.
[0012] Another object of the present invention is to provide a multipurpose biodegradable disinfectant or hand sanitizer composition which is alcohol-free composition.
[0013] Another object of the present invention is to provide a multipurpose biodegradable disinfectant or hand sanitizer composition which provides long lasting antimicrobial activity.
[0014] Another object of the present invention is to provide a multipurpose biodegradable disinfectant or hand sanitizer composition which is highly safe, stable and effective.
[0015] Another object of the present invention is to provide a method of preparation of a multipurpose biodegradable disinfectant or hand sanitizer composition.
[0016] Yet another object of the present invention is that disinfectant composition can be used on the surface of living as well as nonliving things and it gives protection from microbes.

SUMMARY OF THE INVENTION
[0017] The present disclosure generally relates to a disinfectant composition. Specifically, the present invention relates to a multipurpose biodegradable disinfectant composition comprising of amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid, and one or more pharmaceutically or cosmetically acceptable excipients. The disinfectant composition of the present disclosure possesses excellent long lasting antimicrobial, antifungal and virucidal property.
[0018] In one aspect, the present invention relates to a multipurpose biodegradable disinfectant or sanitizer composition comprising of:
(a) amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid;
(b) blue dye;
(c) Citric Acid;
(d) Glycerin;
(e) perfume;
(f) optionally acid thickener and salt solution; and
(g) water.
[0019] In another aspect, the present invention relates to a multipurpose biodegradable disinfectant liquid or hand sanitizer composition comprising of:
(a) amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid in an amount ranges from about 0.1% to about 1.5% by weight of the composition;
(b) 1% blue dye in an amount ranges from about 0.005% to about 0.5% by weight of the composition;
(c) 25% citric acid in an amount ranges from about 0.1% to about 4% by weight of the composition;
(d) glycerin in an amount ranges from about 1% to about 4% by weight of the composition;
(e) perfume in an amount ranges from about 0.1% to about 1% by weight of the composition;
(f) optionally acid thickener in an amount ranges from about 2.5% to about 3.5% by weight of the composition in case of disinfectant sanitizer gel;
(g) optionally salt solution in an amount ranges from about 2% to about 6% by weight of the composition; and
(h) water as required.
[0020] In another aspect of the present invention, the acid thickener is alkoxylated tertiary amine selected from Ethomeen C/12, Ethomeen C/15, Ethomeen C/25, Ethomeen T/12, Ethomeen T/20, Ethomeen T/25, Ethomeen T/30 and Ethomeen T/60.
[0021] In another aspect of the present invention, the Perfume (Refreshing Aroma C0238) is yellow, clear liquid, having specific gravity of 0.905 and refractive index of 1.479. Perfume is soluble in 80% alcohol in 1:1 proportion yielding clear solution. GC pattern matches with the Supplier’s GC chromatogram.
[0022] In another aspect of the present invention, the formulation of the present invention is in the form of liquid, spray, gel, lotion, powder or cream.
[0023] Various objects, features, aspects and advantages of the inventive subject matter will become more apparent from the following detailed description of preferred embodiments.

DETAILED DESCRIPTION OF THE INVENTION
[0024] The following is a detailed description of embodiments of the disclosure. The embodiments are in such detail as to clearly communicate the disclosure. However, the amount of detail offered is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the present disclosure as defined by the appended claims.
[0025] Unless the context requires otherwise, throughout the specification which follow, the word “comprise” and variations thereof, such as, “comprises” and “comprising” are to be construed in an open, inclusive sense that is as “including, but not limited to.”
[0026] Reference throughout this specification to “one embodiment” or “an embodiment” means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases “in one embodiment” or “in an embodiment” in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
[0027] As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural referents unless the content clearly dictates otherwise. It should also be noted that the term “or” is generally employed in its sense including “and/or” unless the content clearly dictates otherwise.
[0028] The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein.
[0029] The headings and abstract of the invention provided herein are for convenience only and do not interpret the scope or meaning of the embodiments.
[0030] All publications herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. Where a definition or use of a term in an incorporated reference is inconsistent or contrary to the definition of that term provided herein, the definition of that term provided herein applies and the definition of that term in the reference does not apply.
[0031] Various terms as used herein are shown below. To the extent a term used in a claim is not defined below, it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.
[0032] The term, “pharmaceutically or cosmetically acceptable excipients” as used herein refers an inactive ingredient in the composition which has no role in the therapeutic effect of the composition however helps in controlling the bio-availability, maintaining the viscosity and improving the stability of the composition.
[0033] As used herein, the compound “amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid” is antimicrobial compound and commonly known as “Ampholyt”.
[0034] As used herein, the “salt solution” refers to solubilizing sodium chloride crystals in demineralized water in required proportion.
[0035] The term, “about” as used herein refers to ±5% of the recited value. For example, about 10% includes 9.5% to 10.5%.
[0036] In a general embodiment, the present invention relates to a disinfectant composition. Specifically, the present invention relates to a multipurpose biodegradable disinfectant composition comprising of amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid, and one or more pharmaceutically or cosmetically acceptable excipients. The disinfectant composition of the present disclosure possesses excellent long lasting antimicrobial, antifungal and virucidal property.
[0037] In an embodiment, the present invention relates to a multipurpose biodegradable disinfectant or sanitizer composition comprising of:
(a) amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid;
(b) blue dye;
(c) Citric Acid;
(d) Glycerin;
(e) perfume;
(f) optionally acid thickener and salt solution; and
(g) water.
[0038] In an embodiment of the present invention, the acid thickener is selected from Ethomeen C/12, Ethomeen C/15, Ethomeen C/25, Ethomeen T/12, Ethomeen T/20, Ethomeen T/25, Ethomeen T/30 and Ethomeen T/60. Preferably, the acid thickener is Ethomeen C/12, Ethomeen C/15, or Ethomeen C/25. More preferably, the acid thickener is Ethomeen C/12.
[0039] In various embodiments, the disinfectant composition of the present invention can be used on the surface of living and non-living things.
[0040] In another embodiment, the disinfectant composition of the present invention can be spread on the porous and non-porous surfaces and keeps the floors free from harmful microbes for up to 4 weeks.
[0041] In some embodiment, the present invention relates to a multipurpose biodegradable gel hand sanitizer composition comprising of:
(a) amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid in an amount ranges from about 0.1% to about 1.5% by weight of the composition;
(b) 1% blue dye in an amount ranges from about 0.005% to about 0.5% by weight of the composition;
(c) 25% citric acid in an amount ranges from about 0.1% to about 4% by weight of the composition;
(d) glycerin in an amount ranges from about 1% to about 4% by weight of the composition;
(e) perfume in an amount ranges from about 0.1% to about 1% by weight of the composition;
(f) optionally, acid thickener in an amount ranges from about 2.5% to about 3.5% by weight of the composition;
(g) optionally, salt solution in an amount ranges from about 2% to about 6% by weight of the composition; and
(h) water as required.
[0042] In yet another embodiment, the present invention relates to a method of preparing a multipurpose biodegradable liquid disinfectant composition comprising the steps of:
(a) adding amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid in water under stirring to obtain homogenous solution;
(b) adding citric acid (for pH to be adjusted between 6-7), glycerin and blue dye one by one to the homogenous solution under stirring to obtain homogenous mixture; and
(c) adding perfume to the homogenous mixture to obtain the multipurpose biodegradable liquid disinfectant composition.
[0043] In yet another embodiment, the present invention relates to a method of preparing a multipurpose biodegradable gel hand sanitizer formulation comprising the steps of:
(a) adding amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid to water under stirring to obtain a homogeneous solution;
(b) adding acid thickener followed by 25% citric acid solution under stirring to obtain a mixture; and
(c) adding glycerin, perfume and dye under stirring to the mixture and adding salt solution slowly and stirring the mixture for 20 minutes to obtain a homogeneous gel as a hand sanitizer composition.
[0044] According to the present invention, the formulation comprising of amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid is water based formulation and free of alcohol. The water-based formulation of the present invention provides immediate and also long-lasting antimicrobial efficacy.
[0045] In another embodiment, the liquid disinfectant formulation of the present invention can be in the form of misting formulation and when sprayed in room gives protection for up to 8 hours thereby reducing the ingestion by users and protects undue exposure to unwanted chemicals.
[0046] In yet another embodiment, the hand sanitizer formulation of the present invention gives protection up to four hours. Hence, the frequency of usage of the present formulations is lessened considerably thereby reduces the chances of sanitizer contact to skin.
[0047] In another embodiment of the present invention, the hand sanitizer formulation comprising of amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid is free of any skin related disorders like drying, whitening, scaling and the like.
[0048] According to the present invention, the disinfectant formulation is stable and there is no decrease in efficacy of the sanitizer after stability conditions.
[0049] In another embodiment of the present invention, the multipurpose biodegradable disinfectant liquid formulation is useful to spray on the porous and non-porous surface, on hands surface, and for the aerial misting purpose. Accordingly, the multipurpose biodegradable disinfectant composition of the present invention is said to be three in one disinfectant composition.
[0050] In another embodiment of the present invention, the biodegradable disinfectant hand sanitizer gel is useful for sanitizing the human hands surface.
[0051] According to the present invention, the disinfectant composition comprising of amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid shows excellent antimicrobial activity. The composition of the present invention provides long lasting antimicrobial effect for up to four hours.
[0052] In another, embodiment of the present invention, the disinfectant composition is a high-level broad-spectrum disinfectant liquid for use on all porous and non-porous surfaces, hands & as aerial mist. It offers safe and effective disinfection for all equipment, surfaces in any setting.
[0053] In yet another embodiment, the disinfectant composition of the present invention is highly effective against bacteria, viruses, fungi, spores, black mould, moss and algae in critical damp areas inside and outside of buildings.
[0054] In another embodiment, the disinfectant composition of the present invention is alcohol-free formulation ensures it is non-toxic, non-irritant, non-corrosive mist on the surfaces killing & destroying up to 99.9% microbes both airborne and static. Due to very good spreading properties on the surface, the treatment is faster than traditional disinfectant methods.
[0055] In various embodiments, the disinfectant composition of the present invention possesses excellent long lasting antimicrobial, antifungal and virucidal property.
[0056] While the foregoing describes various embodiments of the disclosure, other and further embodiments of the disclosure may be devised without departing from the basic scope thereof. The scope of the invention is determined by the claims that follow. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.
[0057] The present invention is further explained in the form of following examples. However, it is to be understood that the following examples are merely illustrative and are not to be taken as limitations upon the scope of the invention.
[0058] Example 1: Multipurpose Disinfectant liquid Composition
Composition:
S. NO INGRIDENTS PERCENT (%)
1. Amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloro acetic acid 1
2. Blue dye 1% 0.015
3. Glycerin 2
4. Perfume (Sporty Fresh (MFN12369) Mayas) 0.4
5. Citric Acid 25% 0.25
6. Water DM Qs

[0059] Manufacturing process
To water add glycerin under stirring and then add Amine, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid under stirring to obtain homogenous solution. Adjust pH of solution from 6-7 by using citric acid (25%) solution. Finally add perfume and then dye.
Characteristics, specification and evaluation report of multipurpose biodegradable disinfectant liquid
Sr. No. Test Unit Specification Observation
Sample-1 Sample-2 Average
1 Colour - Light Blue Light Blue Light Blue Light Blue
2 Appearance - Liquid Liquid Liquid Liquid
3 Odour - As per standard As per standard As per standard As per standard
4 pH @ 290C - 6.0 - 7.0 6.54 6.52 6.53
5 Specific Gravity g/ml 0.9 – 1.2 1.05 1.07 1.06
6 Water Content % 90 % min. 95.4 96.2 95.3

[0060] Stability study of multipurpose biodegradable disinfectant liquid
The disinfectant liquid, example 1 of the present invention was studied for its accelerated storage stability for a period of 8 weeks @ 40 ± 20 c as per CIPAC MT 46.3. The test item was stored at room temperature 0th day (time zero) and at 40 ± 2°C for a period of 8 Weeks. The test item was evaluated for their chemical and physical stability on 0th day and 8 weeks i.e. after completion of storage period. All test parameter after 8 weeks storage at 40 ± 2°C was compared with all test parameter of the 0th day (time zero) analysis.
[0061] Summary of Analytical results of Multipurpose disinfectant Liquid - 0th Day
Sr. No. Test Unit Specification Observation
Sample-1 Sample-2 Average
1 Colour - Light Blue Light Blue Light Blue Light Blue
2 Appearance - Liquid Liquid Liquid Liquid
3 Odour - As per standard As per standard As per standard As per standard
4 pH @ 290C - 6.0 - 7.0 6.62 6.66 6.64
5 Specific Gravity g/ml 0.9 – 1.2 1.01 1.03 1.02
6 Water Content % 90 % min. 95.6 96.2 95.4

[0062] Summary of Analytical results of Multipurpose disinfectant Liquid after 8 weeks @ 40 ± 2?C
Sr. No. Test Unit Specification Observation
Sample-1 Sample-2 Average
1 Colour - Light Blue Light Blue Light Blue Light Blue
2 Appearance - Liquid Liquid Liquid Liquid
3 Odour - As per standard As per standard As per standard As per standard
4 pH @ 290C - 6.0 - 7.0 6.50 6.52 6.51
5 Specific Gravity g/ml 0.9 – 1.2 1.08 1.06 1.07
6 Water Content % 90 % min. 95.2 95.4 95.3

The stored product showed no significant chemical and physical changes after storage for 8 weeks storage at 40 ± 2°C. Based on the chemical and physical results obtained from the study, it can be concluded that the sample is stable after storage at 40 ± 2°C temperature for 8 weeks. This accelerated storage test provides useful guidance on performance after storage as the product passes the accelerated storage test and has satisfactory shelf life (of at least 2 years) in hot or temperate climates.
[0063] EFFICACY DATA
[0064] Antiviral activity of Multi-Purpose Disinfectant Liquid against non-envelope virus: The study on quantitative Assessment of Antiviral Activity is performed by following ISO 21702: 2019. Test sample was applied on ceramic surface and allowed to dry. Pre-sterilized samples were loaded with diluted viral suspension to 106 PFU/ ml. Virus suspension 0.4 ml was added to 50 mm x 50 mm of Test substrate. It was covered with 40 mm x 40 mm LDPE film. Following exposure time, Virus was eluted and neutralized by serial tenfold dilution and assayed to determine surviving Viruses in comparison with Control without test product in sq. cms. Virus assay was quantitative as Plaque forming unit (PFU) visible as area of Clearance
Results:
A. After 1 hour of ageing
1. Contact time at 10 minutes
Parameter Mean of 3 replicates
Average number of plaques recovered from treated (At) 1011
Log of Plaques recovered from Treated (At) Log At 3.0
Antiviral Activity ® (log Ut- At) 1.8
Virus Reduction Percentage (%) 98.74
Untreated: Average number of Plaques recovered at 0 hours (U0): 6.20 x 104 PFU/sq. cm. (Log = 4.79); Untreated: Average number of Plaques recovered at 10 minutes (Ut): = 8.00 x 104 PFU/sq. cm. (Log = 4.90).

2. Contact time at 1 hour
Parameter Mean of 3 replicates
Average number of plaques recovered from treated (At) 1716
Log of Plaques recovered from Treated (At) Log At 3.2
Antiviral Activity ® (log Ut- At) 1.6
Virus Reduction Percentage (%) 97.9
Untreated: Average number of Plaques recovered at 0 hours (U0): 6.20 x 104 PFU/sq. cm. (Log = 4.79); Untreated: Average number of Plaques recovered at 1 hours (Ut): = 8.20 x 104 PFU/sq. cm. (Log = 4.91).
3. Contact time at 24 hours
Parameter Mean of 3 replicates
Average number of plaques recovered from treated (At) 2503
Log of Plaques recovered from Treated (At) Log At 3.4
Antiviral Activity (R) 1.5
Virus Reduction Percentage (%) 97
Untreated: Average number of Plaques recovered at 0 hours (U0): 6.20 x 104 PFU/sq. cm. (Log = 4.79); Untreated: Average number of Plaques recovered at 24 hours (Ut): = 8.50 x 104 PFU/sq. cm. (Log = 4.92).
B. After 24 hour of ageing
1. Contact time at 10 minutes
Parameter Mean of 3 replicates
Average number of plaques recovered from treated (At) 1588
Log of Plaques recovered from Treated (At) Log At 3.2
Antiviral Activity (R) 1.7
Virus Reduction Percentage in % 98.2
Untreated: Average number of Plaques recovered at 0 hours (U0): 6.10 x 104 PFU/sq. cm. (Log = 4.78); Untreated: Average number of Plaques recovered at 10 minutes (Ut): = 9.00 x 104 PFU/sq. cm. (Log = 4.95)
2. Contact time at 1 hour
Parameter Mean of 3 replicates
Average number of plaques recovered from treated (At) 2000
Log of Plaques recovered from Treated (At) Log At 3.3
Antiviral Activity (R) 1.6
Virus Reduction Percentage in % 97.8
Untreated: Average number of Plaques recovered at 0 hours (U0): 6.10 x 104 PFU/sq. cm. (Log = 4.78); Untreated: Average number of Plaques recovered at 1 hours (Ut): = 9.10 x 104 PFU/sq. cm. (Log = 4.95)
3. Contact time at 24 hour
Parameter Mean of three replicates
Average number of plaques recovered from treated (At) 3333
Log of Plaques recovered from Treated (At) Log At 3.52
Antiviral Activity (R) 1.38
Virus Reduction Percentage in % 96.08
Untreated: Average number of Plaques recovered at 0 hours (U0): 6.20 x 104 PFU/sq. cm. (Log = 4.78); Untreated: Average number of Plaques recovered at 24 hours (Ut): = 8.50 x 104 PFU/sq. cm. (Log = 4.97)
Note: Ageing – Incubation of plate at 37 °C for MS2 Bacteriophage virus in the CO2 incubator
Where:
R = Antiviral activity
U0 = Log of PFU recovered from Untreated specimen immediately after inoculation, in PFU/ cm2
Ut = Log of PFU recovered from Untreated specimen after 10 mins/ 1 hrs / 24 hrs. after inoculation, in PFU/ cm2
At = Log of PFU recovered from Treated specimen after 10 mins/ 1 hrs / 24 hrs. after inoculation, in PFU/ cm2
Conclusion: It is concluded from the study that Multi-Purpose Disinfectant Liquid has shown 97.05% reduction of virus in 24 hours contact time initially at 1hour of ageing & 96.08 % reduction of virus in 24 hours contact time at 24 hour of ageing when tested by ISO 21702: 2019 standard.
[0065] Antiviral efficacy of Multi-Purpose Disinfectant Liquid against envelope virus
Test Procedure:
Test sample was applied on ceramic surface and allowed to dry. Pre-sterilized samples were loaded with diluted viral suspension. Virus suspension, 0.4 ml was added to 50 mm x 50 mm of Test surfaces. It was covered with 40 mm x 40 mm LDPE film. After the completion of contact time, virus was eluted and neutralized by serial tenfold dilution using neutralizing medium and assayed to determine surviving virus in comparison with Control without test product in sq. cm. Virus assay was quantitative as Plaque forming unit (PFU) visible as area of Clearance.
Results
Virus Contact Duration Group Logarithm of infectivity titer of virus
(Log TCID50/cm2) Average titer Infectivity of virus (Log TCID50/cm2)
Influenza virus suspension: (2.50 × 108 PFU / ml) 0 hours Control (Uo) R 1 5.44 5.49
R 2 5.4
R 3 5.62
24 hours Control (Ut) R 1 5.3 5.45
R 2 5.48
R 3 5.56
24 hours Test (At) R 1 2.5 2.20
R 2 2
R 3 2.1
Antiviral Activity R = Ut – At
(24 hours contact) - 3.25 (99.9%)

Where:
R = Antiviral activity
R1/R2/R3=Replications
U0 = is the average of common logarithmic from three control/ untreated specimen immediately after inoculation
Ut = is the average of common logarithmic from three control/ untreated specimen immediately after 24 hours
At = is the average of common logarithmic from three treated specimen after 24 hours
Conclusion:
It is concluded from the study that Multi-Purpose Disinfectant Liquid has shown 99.9 % reduction of virus in 24 hours of contact period when applied on ceramic surface and tested using ISO 21702: 2019 standard.
[0066] Quantitative suspension evaluation multipurpose disinfectant liquid for basic bactericidal activity against Pseudomonas aeruginosa and Staphylococcus aureus using EN 1040:2005 method and basic fungicidal activity against Aspergillus niger and Candida albicans using EN 1605:2005 method
Data:
Test Organism Control (cfu/ml) Log10 value Contact time 1 min. 2 min. 5 min.
Staphylococcus aureus 20000000


7.3

Test (CFU/ml) 9300 3533 166
Log10 4.0 3.5 2.2
Log reduction 3.3 3.8 5.1
% Reduction 100.0 100.0 100.0
Pseudomonas aeruginosa 16000000


7.2

Test (CFU/ml) 9600 7500 166.6
Log10 4.0 3.9 2.2
Log reduction 3.2 3.3 5.0
% Reduction 99.9 100.0 100.0

Test Organism Control (cfu/ml) Log10 value Contact time 5 min. 15 min. Interfering substance (dirty condition)
Aspergillus brasiliensis
1500000
6.18


Test (CFU/ml) 5266 1166 3 g/l bovine albumin solution
Log10 3.7 3
Log reduction 2.48 3.18
% Reduction 99.6 99.9
Candida albicans
2100000
6.32
Test (CFU/ml) 5533 1133 3 g/l bovine albumin solution

Log10 3.7 3.1
Log reduction 2.6 3.3
%Reduction 99.7 99.9
Data: Average of three replicates
Conclusion: It is concluded from the study that multipurpose disinfectant liquid exhibited bactericidal activity by demonstrated a log reduction of more than 5 logs and achieved greater than 99.999 % in Staphylococcus aureus and 99.999 % in Pseudomonas aeruginosa of percent log reduction under test conditions at 5 minutes of contact time. It has also exhibited fungicidal activity demonstrated by a log reduction of 3 and achieved greater than 99.9 % in both Aspergillus brasiliensis and Candida albicans of percent log reduction under test conditions at 15 minutes of contact time.
[0067] Quantitative Suspension test of multipurpose disinfectant liquid for basic Sporicidal activity against Clostridium perfringes and Bacillus cereus spores using EN 13704:2018 method:
Data:

Test Organism Test Method Control (cfu/ml) Log10 value Contact time (Up to 60 min.) 5 min. 30 min. 60 min.
Clostridium perfringes


EN 13704 - Dilution neutralization Sporicidal phase 2 step 1 15500000


7.19


CFU/ml 29800 21800 5866
Log10 3.8 4.3 3.7
Log reduction 2.7 2.83 3.4
% Reduction 99.8 99.8 99.9
Bacillus cereus


EN 13704 - Dilution neutralization Sporicidal phase 2 step 1 11000000


7.04


CFU/ml 27700 11100 6866.6
Log10 4.4 4.0 3.8
Log reduction 2.5 2.9 3.2
%Reduction 99.7 99.8 99.9
Interfering substance (dirty condition): 3 g/l bovine albumin solution; Data is from three replicates
Conclusion: It is concluded from the study that multipurpose disinfectant liquid exhibited sporicidal activity demonstrated by a log reduction of 3 and achieved greater than 99.9 percent log reduction under test conditions at 60 minutes of contact time for both Clostridium perfringes and Bacillus cereus. Therefore, multipurpose disinfectant liquid is 99.9% effective against spores are said to have achieved 3-log reduction against those microbes.
[0068] Antimicrobial efficiency & longevity of Multipurpose Disinfectant Liquid against Bacteria such as; Pseudomonas aeruginosa and Staphylococcus aureus and fungi such as; Aspergillus brassiliences and Candida albicans after freshly spraying on Porous and Non-Porous Surface by following standard method of JIS Z 2801: 2010
Staphylococcus aureus
(% Reduction) Pseudomonas aeruginosa
(% Reduction)
Porous Non porous Porous Non porous
Surfaces Cement Wooden Ceramic Stainless steel Cement Wooden Ceramic Stainless steel
0 Day 99.99 99.99 99.96 99.95 99.99 99.99 99.9 99.99
1 Week 99.95 99.94 99.94 99.93 99.99 99.99 99.9 99.99
2 Week 99.9 99.9 99.9 99.91 99.9 99.9 99.9 99.9
3 Week 99.92 99.92 99.93 99.93 99.9 99.91 99.9 99.91
4 Week 99.92 99.94 99.92 99.93 99.9 99.9 99.91 99.92
5 Week 98.93 98.95 98.84 98.87 98.81 98.72 98.65 98.66

Aspergillus brasiliensis
(% Reduction) Candida albicans
(% Reduction)
Porous Non porous Porous Non porous
Surfaces Cement Wooden Ceramic Stainless steel Cement Wooden Ceramic Stainless steel
0 Day 99.99 99.99 99.98 99.99 99.99 99.99 99.95 99.94
1 Week 99.98 99.98 99.97 99.97 99.93 99.93 99.92 99.92
2 Week 99.95 99.95 99.94 99.95 99.94 99.95 99.95 99.96
3 Week 99.9 99.91 99.91 99.9 99.95 99.95 99.95 99.96
4 Week 99.91 99.92 99.92 99.92 99.92 99.96 99.93 99.94
5 Week <90.00 <90.00 <90.00 <90.00 98.9 99 98.92 98.94

Conclusion: It is concluded from the results of this study that the Multipurpose Disinfectant Liquid exhibited in test organisms, showing 99.9% bacterial and fungal growth reduction over control under the test surfaces and conditions employed in a given contact time up to 4 weeks.
[0069] Microbiological efficacy testing of multipurpose disinfectant liquid on Bacteria and Fungus on hands
Reference method: ASTM E2276 – Bacterial Finger pad Method and ASTM E2613- Fungicidal Finger pad Method. Bacterial Strains: Escherichia coli ATCC 8739; Staphylococcus aureus ATCC 6538; Fungal Strains: Aspergillus brasiliensis ATCC 16404; Candida albicans ATCC 10237
For bacterial, number cfu's per plate from dilution 10-5 count obtained from 0.1 ml volume of culture plate and for fungi, number of cfu's per plate from dilution 10-4 count obtained from 0.1 ml volume of culture plate
Organism Staphylococcus aureus Pseudomonas aeruginosa Aspergillus niger Candida albicans
Suspension (Cells/ml) 1.5 x 106 5.00 x 105 4.00 x 105
Subject
Thumb pad L /R No of cfu's per plate from dilution 10-5 count obtained from 0.1 ml volume of culture plate No of cfu's per plate from dilution 10-4 count obtained from 0.1 ml volume of culture plate
Initial Count of Test Microorganism (Pre-Values) Count of surviving Test Microorganism (Post - values) Initial Count of Test Microorganism (Pre-Values) Count of surviving Test Microorganism (Post - values) Initial Count of Test Microorganism (Pre-Values) Count of surviving Test Microorganism (Post - values) Initial Count of Test Microorganism (Pre-Values) Count of surviving Test Microorganism (Post - values)
1 L 267000000 1000000 234000000 0 13400000 0 11900000 0
R 158000000 0 124000000 1000000 14500000 0 13600000 0
2 L 194000000 0 185000000 0 13200000 0 12700000 0
R 271000000 0 219000000 0 12200000 0 10700000 0
3 L 284000000 0 273000000 0 13200000 0 13200000 100000
R 291000000 0 246000000 0 12600000 0 10900000 0
4 L 278000000 0 148000000 0 14500000 0 13400000 0
R 257000000 0 22000000 0 13800000 0 13700000 0
5 L 196000000 0 136000000 0 13200000 0 11900000 0
R 286000000 0 245000000 0 12900000 100000 16400000 0
Average 248200000 100000 203000000 100000 13350000 10000 12840000 10000
Log10 8.39 5 8.31 5 7.13 4 7.11 4
Log Reduction 3.39 3.31 3.13 3.11
% Reduction 99.96 99.95 99.93 99.92
Results: The test product, multipurpose disinfectant liquid of the present invention is effective towards all the test organisms i.e. Bacteria - Pseudomonas aeruginosa & Staphylococcus aureus and Fungal - Aspergillus niger & Candida albicans and has demonstrated a log reduction of more than 3 logs and has achieved more than 99.9% reduction.
[0070] Antimicrobial efficiency & longevity of Multipurpose Disinfectant Liquid against Pseudomonas aeruginosa, Salmonella abony, Escherichia coli and Staphylococcus aureus after freshly spraying in closed glass chamber against air borne microbes.
Method: This method is based on USP<1116> Microbiological Evaluation of clean rooms. This test was conducted in 6 m3 Glass chamber using settle plates method (Passive monitoring uses “settle plates”, which are standard Petri dishes containing culture media, which are exposed to the air for a given time in order to collect biological particles which “sediment” out and are then incubated.) Two chambers were used for the study, before initiating the study the chambers were cleaned. One chamber was used as control chamber and the plates inoculated with Bacteria separately were placed in the chamber on each wall in the centre and at a height of 2 ft from the ground. The plates were exposed in the same environment for 4 hours and then removed and incubated for 24 hours and 48 hours.
In the second chamber, the product (1% concentration) was sprayed using a mechanical spraying machine for 10 seconds and releasing 95 grams in 6 m3 chamber (15.8 grams / m3) and the plates inoculated with Bacteria separately were exposed after 1 hour from spray in the chamber on each wall in the centre and at a height of 2 ft from the ground. The plates were exposed in the same environment for 4 hours (exposure time inside the chamber was for 1 hour) and then removed and incubated for 24 hours and 48 hours.
For checking the residual impact, the settle plates inoculated with bacteria and fungus were exposed after 4 hour, 8 hour 12 hour and 24 hours from start.
1 Hr after Misting 4 Hr after Misting 8 Hr. after Misting 12 Hr. after Misting 24 Hr after Misting
Staphylococcus aureus Initial count of test organism per ml of test mixture (Cfu/ml) 33000000 33000000 33000000 33000000 33000000
Log10 7.52 7.52 7.52 7.52 7.52
Count of surviving test microorganism per ml (Cfu/ml) 2700 2500 6700 2100000 2600000
Log10 3.43 3.4 3.83 6.32 6.41
Log Reduction 4.09 4.12 3.69 1.2 1.11
% kill of test organism 99.99 99.99 99.90 90 90
Escherichia coli Initial count of test organism per ml of test mixture (Cfu/ml) 33000000 33000000 33000000 33000000 33000000
Log10 7.52 7.52 7.52 7.52 7.52
Count of surviving test microorganism per ml (Cfu/ml) 2500 2300 3700 2100000 1800000
Log10 3.4 3.36 3.57 6.32 6.26
Log Reduction 4.12 4.16 3.95 1.2 1.26
% kill of test organism 99.99 99.99 99.90 90 90
Salmonella abony Initial count of test organism per ml of test mixture (Cfu/ml) 33000000 33000000 33000000 33000000 33000000
Log10 7.52 7.52 7.52 7.52 7.52
Count of surviving test microorganism per ml (Cfu/ml) 2600 2100 3700 2300000 2100000
Log10 3.41 3.32 3.57 6.36 6.32
Log Reduction 4.11 4.2 3.95 1.16 1.2
% kill of test organism 99.99 99.99 99.90 90 90
Pseudomonas aeruginosa Initial count of test organism per ml of test mixture (Cfu/ml) 33000000 33000000 33000000 33000000 33000000
Log10 7.52 7.52 7.52 7.52 7.52
Count of surviving test microorganism per ml (Cfu/ml) 2500 2000 3400 1000000 1300000
Log10 3.4 3.3 3.53 6 6.11
Log Reduction 4.12 4.22 3.99 1.52 1.41
% kill of test organism 99.99 99.99 99.90 90 90

CONCLUSION: It is concluded from results of this study that Multipurpose Disinfectant Liquid exhibited in test organisms, showing 99.9% bacterial growth reduction over control in all strains under the test conditions employed up to 8 hours.
[0071] Antimicrobial activity of multipurpose disinfectant liquid using reduction of total viable count of microbes on volunteer’s hand for instant and residual protection
Method: The Hand sanitizers were tested with volunteers. About 3 ml of the hand sanitizer liquid were asked to use by the volunteers for 30 seconds in a defined process to examine the efficacy of the hand disinfectant. After applying the hand sanitizer, Petri plates with Soybean casein digest agar were opened and the volunteers were asked to place their palm on the labelled plates at fresh and after 15 minutes, 1hr, 2hr, 3hr, 4hr, and 5hr after application. Similarly, volunteer meant for untreated control were asked to place their palm. The plates were closed immediately and incubated at 35°C for 24 hours. After incubation period, colonies were counted and expressed as percent inhibition over control plates
Multipurpose Disinfectant Liquid
Total Viable Count of microbes (CFU)/plate
Residual Time point Initial Count Untreated Control
(cfu / Plate) Count of surviving
Test Product
(cfu / Plate) * Log Reduction % Reduction
Initial >300 0.04 4 99.99
15 min. 289 0.04 4 99.99
1 Hr. 278 0.04 4 99.99
2 Hr. 296 0.24 3 99.92
3 Hr. 287 0.20 3 99.93
4 Hr. 286 0.28 3 99.91
5 Hr. 295 28.80 1 90.24
* Mean of 4 volunteers
Conclusion: It is concluded from the study that multipurpose disinfectant liquid exhibited 99.99% reduction up to 1 hour after application and further from 2 hours up to 4 hours the reduction was found to be 99.9% and at 5 hours it dipped down to 90% reduction. This proves that the product is effective up to 4 hours with 99.9% reduction.
[0072] Antimicrobial efficiency & surface longevity of multipurpose Disinfectant liquid against algal species after freshly spraying up to 5 weeks on porous and nonporous surfaces
Algal cells with a cell density of 1.20×104 cells/ml was added to each surface with a volume of 0.4ml/surface separately on each surfaces. Then the surfaces were exposed with test sample. The inoculum added in 0-hour surface (untreated) was washed out immediately with 10 mL of salt and buffer solution and the washed-out solutions inoculated in agar plates and incubated at 25°C. Similarly, the surfaces meant for 1, 2, 3, 4 up to 5 weeks were incubated at 25°C after addition of inoculum on the surfaces. After each incubation, 1, 2, 3, 4 up to 5 weeks the surfaces were washed-out with salt and buffer solution, the dilutions were inoculated in bolds basal medium and incubated for 7 days. The number of cells in each flasks were enumerated by haemocytometer.
Summary of % Reduction observed:
Duration Chlorella species (% Reduction)
Porous Surface Non-Porous Surface
Cement Wooden Ceramic Stainless steel
0 Day 99.97 99.95 99.97 99.98
1 Week 99.96 99.92 99.99 99.99
2 Week 99.91 99.89 99.99 99.99
3 Week 99.90 99.89 100.00 100.00
4 Week 99.90 99.88 100.00 100.00
5 Week 99.74 99.87 100.00 100.00

Conclusion: The results of this study demonstrated that the multipurpose Disinfectant liquid of the present invention exhibited >99 % growth reduction in Chlorella species over control and it gives extended protection up to 5 weeks.
[0073] Antimicrobial efficiency & surface longevity of Multipurpose Disinfectant liquid Product against Moss species after freshly spraying up to 5 weeks on porous and nonporous surfaces
Approximately 2 g weight of Moss biomass was applied to each test surface separately. Then the surfaces were exposed with test sample. Similarly, the surfaces meant for 1, 2, 3, 4 up to 5 weeks were incubated at 28°C after addition of inoculum on the test surfaces. After each incubation, 1, 2, 3, 4 up to 5 weeks the surfaces were washed-out with scrapper and collected biomass were weighed.

Results: Summary of % Reduction observed
Moss (% Reduction)
Porous Surface Non-Porous Surface
Duration Cement Wooden Ceramic Stainless steel
1 Week 99.90 99.99 99.99 99.99
2 Week 80.00 90.00 0 0
3 Week 70.30 80.00 0 0
4 Week 67.47 74.75 0 0
5 Week 74.28 70.06 0 0

Conclusion: It is concluded from results of this study that the Surfactant Disinfectant liquid of the present invention exhibited 99.9 % growth reduction in cement surface, wooden, ceramic, stainless steel over control up to 1 week.
[0074] Example 2: Disinfectant Hand sanitizer Composition
S.NO INGRIDENTS PERCENT (%)
1. Amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloro acetic acid 1
2. Blue dye 1% 0.03
3. Glycerin 2
4. Perfume (Refreshing Aroma C0238) 0.3
5. Citric Acid 25% 2.9
6. Ethomeen C/12 3
7. Salt solution 5
8. Water DM Qs

[0075] Manufacturing Process
To water add Amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloro acetic acid under stirring for having homogeneous solution. Then add Acid Thickener and then add Citric acid solution under stirring. Subsequently addition of glycerin, perfume and dye is done under stirring. Finally, add salt solution slowly and keep under stirring for 20 min. for having homogeneous gel without any lumps. Leave it for 2-4 hr. and then pack.
[0076] Characteristics, specification and evaluation report of multipurpose biodegradable disinfectant hand sanitizer
Sr. No. Test Unit Specification Observation
Sample-1 Sample-2 Average
1 Colour - Light Blue Light blue Light Blue Light Blue
2 Appearance - Gel Gel Gel Gel
3 Odour - As per standard As per standard As per standard As per standard
4 pH @ 29?C - 5.0 -7.0 5.91 5.89 5.90
5 Specific Gravity g/ml 0.9 – 1.2 1.05 1.07 1.06
6 Water Content % 90 % min. 93.1 93.5 93.30
7 Viscosity (By Brookfield Viscometer) Spindle number 5 speed 10 rpm @ 25?C) Cps Min 80 160 172 166

[0077] Stability study of multipurpose biodegradable disinfectant hand sanitizer
The test item, disinfectant hand sanitizer of the present invention was studied for its accelerated storage stability for a period of 8 weeks @ 40 ± 20 c as per CIPAC MT 46.3. Studies were conducted by Chemistry Department, Ross Lifescience Pvt. Ltd. Pune. The test item was stored at room temperature 0th day (time zero) and at 40 ± 2°C for a period of 8 Weeks. The test item was evaluated for their chemical and physical stability on and 8 weeks i.e. after completion of storage period. All test parameter after 8 weeks storage at 40 ± 2°C was compared with all test parameter of the 0th day (time zero) analysis.

[0078] Summary of Analytical results of disinfectant hand sanitizer - 0th Day
Sr. No. Test Unit Specification Observation
Sample-1 Sample-2 Average
1 Colour - Light Blue Light Blue Light Blue Light Blue
2 Appearance - Gel Gel Gel Gel
3 Odour - As per standard As per standard As per standard As per standard
4 pH @ 29?C - 5.0 -7.0 5.91 5.93 5.92
5 Specific Gravity g/ml 0.9 – 1.2 1.09 1.07 1.08
6 Water Content % 90 % min. 93.4 93.6 93.5
7 Viscosity (By Brookfield Viscometer) Spindle number 5 speed 10 rpm @ 25?C) Cps Min 80 172 176 174

[0079] Summary of Analytical results of the disinfectant hand sanitizer after 8 weeks @ 40 ± 2?C
Sr. No. Test Unit Specification Observation
Sample-1 Sample-2 Average
1 Colour - Light Blue Light Blue Light Blue Light Blue
2 Appearance - Gel Gel Gel Gel
3 Odour - As per standard As per standard As per standard As per standard
4 pH @ 29?C - 5.0 -7.0 5.96 5.99 5.98
5 Specific Gravity g/ml 0.9 – 1.2 1.02 1.04 1.03
6 Water Content % 90 % min. 93.7 93.5 93.6
7 Viscosity (By Brookfield Viscometer) Spindle number 5 speed 10 rpm @ 25?C) Cps Min 80 170 176 173
[0080] The stored product showed no significant chemical and physical changes after storage for 8 weeks storage at 40 ± 2°C. Based on the chemical and physical results obtained from the study, it can be concluded that the sample is stable after storage at 40 ± 2°C temperature for 8 weeks. This accelerated storage test provides useful guidance on performance after storage as the product passes the accelerated storage test and has satisfactory shelf life (of at least 2 years) in hot or temperate climates.
[0081] Efficacy Data
Active moiety (viz. amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid) used in both the formulations namely; Multipurpose biodegradable disinfectant liquid and Disinfectant Hand sanitizer Composition is same and in similar proportion (1%). Therefore biological efficacy data generated for both formats is more or less same without any considerable differences.
[0082] A skilled artisan will appreciate that the quantity and type of each ingredient can be used in different combinations or singly. All such variations and combinations would be falling within the scope of present disclosure.

,CLAIMS:1. A multipurpose biodegradable disinfectant liquid composition comprises:
(a) amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid;
(b) blue dye;
(c) citric Acid;
(d) glycerin;
(e) Perfume (Refreshing Aroma C0238); and
(f) water.

2. The multipurpose biodegradable disinfectant liquid composition as claimed in claim 1, wherein the composition comprises:
(a) amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid in an amount ranges from about 0.1% to about 1.5% by weight of the composition;
(b) 1% blue dye in an amount ranges from about 0.005% to about 0.5% by weight of the composition;
(c) 25% citric acid in an amount ranges from about 0.1% to about 4% by weight of the composition;
(d) glycerin in an amount ranges from about 1% to about 4% by weight of the composition;
(e) perfume (sporty fresh (MFN12369) mayas) in an amount ranges from about 0.1% to about 1% by weight of the composition; and
(f) water as required.

3. A method of preparing the multipurpose biodegradable disinfectant liquid composition as claimed in claim 1, wherein the method comprising the steps of:
(a) adding glycerin to water under stirring followed by adding amine, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid under stirring to obtain homogenous solution;
(b) adjusting pH of the homogenous solution in the range of 6-7 using 25% citric acid to obtain a mixture;
(c) finally adding Perfume (Sporty Fresh (MFN12369) Mayas)followed by dye to obtain the multipurpose disinfectant composition as liquid composition.

4. The multipurpose biodegradable disinfectant liquid composition as claimed in claim 1, wherein the formulation is in the form of liquid, spray, gel, lotion, powder or cream and is biodegradable, alcohol-free and stable.

5. A disinfectant hand sanitizer composition comprises:
(a) amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid;
(b) blue dye;
(c) glycerin;
(d) Perfume (Refreshing Aroma C0238);
(e) citric Acid;
(f) ethomeen C/12;
(g) salt solution; and
(h) water.

6. The disinfectant hand sanitizer composition as claimed in claim 5, wherein the composition comprises;
(a) amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid in an amount ranges from about 0.1% to about 1.5% by weight of the composition;
(b) 1% blue dye in an amount ranges from about 0.005% to about 0.5% by weight of the composition;
(c’) 25% citric acid in an amount ranges from about 0.1% to about 4% by weight of the composition;
(d) glycerin in an amount ranges from about 1% to about 4% by weight of the composition;
(e) perfume (refreshing Aroma C0238) in an amount ranges from about 0.1% to about 1% by weight of the composition; and
(f) acid thickener in an amount ranges from about 2.5% to about 3.5% by weight of the composition in case of disinfectant sanitizer gel;
(g) salt solution in an amount ranges from about 2% to about 6% by weight of the composition; and
(h) water as required.

7. A method for preparation of disinfectant hand sanitizer composition as claimed in claim 5, the method comprises the steps of:
(a) adding amines, N-C10–C16-alkyltrimethylenedi-, reaction products with chloroacetic acid to water under stirring to obtain a homogeneous solution;
(b) adding acid thickener followed by 25% citric acid solution under stirring to obtain a mixture;
(c) adding glycerin, perfume (refreshing aroma C0238) and dye under stirring to the mixture and adding salt solution slowly and stirring the mixture for 20 minutes to obtain a homogeneous gel as a hand sanitizer composition.

8. The disinfectant hand sanitizer composition as claimed in claim 5, wherein the composition is in the form of liquid, spray, gel, lotion, powder or cream and is biodegradable, alcohol-free and stable.

9. The biodegradable disinfectant liquid composition as claimed in claim 1, and disinfectant hand sanitizer composition as claimed in 5, wherein the composition are excellent in controlling harmful organisms for extended duration; wherein, compositions give 24 hours protection from non-envelop and envelop viruses; whereas, from Bacteria the protection is sought for extended duration viz., 4 hours protection on hands, 6 weeks protection on surface and 8 hours in aerial conditions; whereas, for fungi protection is sought for extended duration, viz., 4 weeks on surface and 8 hours in aerial conditions; whereas, the protection from Algae is sought for 5 weeks; from Moss 1 week and 60 minutes from Bacterial Spores.