Claims:We claim:
1. A method of synthesizing a multivitamin and mineral composition, comprises:
mixing
a guava leaves extract powder type 1, wherein the guava leaves extract powder type 1 is obtained by a process (1),
a guava leaves extract powder type 2, wherein the guava leaves extract powder type 2 is obtained by a process (2),
a dried lemon peel extract powder type 1, wherein the dried lemon peel extract powder type 1 is obtained by a process (3),
a dried lemon peel extract powder type 2, wherein the dried lemon peel extract powder type 2 is obtained by a process (4),
a powder of amla fruit extract, wherein the powder of amla fruit extract is obtained by a process (5),
a powder of sesbania grandiflora leaves extract, wherein the powder of sesbania grandiflora leaves extract is obtained by a process (6),
a powder of holy basil leaves extract, wherein the powder of holy basil leaves extract is obtained by a process (7),
a powder of annatto seeds extract type 1, wherein the powder of annatto seeds extract type 1 is obtained by a process (8),
a powder of annatto seeds extract type 2, wherein the powder of annatto seeds extract type 2 is obtained by a process (9),
a powder of moringa leaves extract type 1, wherein the powder of moringa leaves extract type 1 is obtained by a process (10),
a powder of moringa leaves extract type 2, wherein the powder of moringa leaves extract type 2 is obtained by a process (11), to obtain a mixture;
milling and blending the mixture;
sifting the mixture through a 40 mesh sifter; and
packing the mixture in an air tight container.

2. The method as claimed in claim 1, wherein the process (1) comprises the steps of:
taking a raw material, wherein the raw material is bio-fortified dried guava leaves;
crushing the dried leaves to a size in the range of 8mm - 10mm;
carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water added in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
concentrating the supernatant;
passing the filtrate through a column packed with ion exchange resin (Amberlite);
eluting the column with acidic water (5% organic lemon juice in water) and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.

3. The method as claimed in claim 1, wherein the process (2) comprises the steps of:
taking a raw material, wherein the raw material is bio-fortified dried guava leaves;
crushing the dried leaves to a size in the range of 8mm - 10mm;
carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water containing 5% lemon juice added in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
passing the filtrate through a column packed with cation exchange resin;
eluting the column with acidic water (5% organic lemon juice in water) and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%; and
spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.

4. The method as claimed in claim 1, wherein the process (3) comprises the steps of:
taking a raw material, wherein the raw material is bio-fortified dried lemon peel;
crushing the dried lemon peel to a size in the range of 8mm - 10mm using a hammer mill;
carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water added in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
passing the filtrate through a column packed with ion exchange resin (Amberlite);
eluting the column with acidic water (5% organic lemon juice in water) and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.

5. The method as claimed in claim 1, wherein the process (4) comprises the steps of:
taking a raw material, wherein the raw material is bio-fortified dried lemon peel;
carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water containing 5% lemon juice added in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
dissolving the filtrate from column elution in 50% ethanol;
passing the filtrate through a column packed with macro porous absorbent resin;
eluting the column with different percentage of ethanol, wherein different percentage of ethanol is in the range of 50-80%, and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%; and
spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.

6. The method as claimed in claim 1, wherein the process (5) comprises the steps of:
taking a raw material, wherein the raw material is bio-fortified amla fruit;
crushing the raw material, squeezing the raw material and filtering the raw material to obtain a juice;
carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the juice through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
passing the filtrate through a column packed with poly-4-vinylpyridine ion exchange resin;
eluting the column with hot water in presence of 5% lemon water;
collecting the eluate and concentrating the eluate at 70°C to 80°C to obtain TDS of 30 to 40%;
spray drying the eluate at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
7. The method as claimed in claim 1, wherein the process (6) comprises the steps of:
taking a raw material, wherein the raw material is bio-fortified dried sesbania grandiflora leaves;
crushing the dried lemon peel to a size in the range of 8mm - 10mm using a hammer mill;
carrying out an extraction of the raw material, wherein the extraction is carried out by using the raw material and aqueous ethanol mixed in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and aqueous ethanol, repeating the extraction step one more time and combining the filtrates;
carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
passing the filtrate through a column packed with agarose polyaldehyde beads resin;
eluting the column with 9% lemon juice and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.

8. The method as claimed in claim 1, wherein the process (7) comprises the steps of:
taking a raw material, wherein the raw material is bio-fortified dried holy basil leaves;
crushing the raw material to a size in the range of 8mm - 10mm using a hammer mill;
carrying out an extraction of the raw material, wherein the extraction is carried out by using the raw material and aqueous ethanol mixed in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and aqueous ethanol, repeating the extraction step one more time and combining the filtrates, and concentrate to 4:1 ratio;
adding 50% ethanol and keeping the concentrate over night, and collecting a supernatant;
carrying out a membrane filtration of the supernatant, wherein the membrane filtration carried out by passing the supernatant through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately, and concentrate the supernatant;
passing the filtrate through a column packed with ion exchange resin (Amberlite);
eluting the column with acidic water (5% organic lemon juice in water), and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.

9. The method as claimed in claim 1, wherein the process (8) comprises the steps of:
taking a raw material, wherein the raw material is annatto seeds;
crushing the raw material to a size in the range of 8mm - 10mm using a hammer mill;
carrying out an extraction of the raw material, wherein the extraction is carried out by using the raw material and aqueous ethanol mixed in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and aqueous ethanol, repeating the extraction step one more time and combining the filtrates;
carrying out a membrane filtration of the supernatant, wherein the membrane filtration carried out by passing the supernatant through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately, and concentrate the supernatant;
passing the filtrate through a column packed with non polar polystyrene adsorbent (SM-2 resin);
eluting the column with ethanol, and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.

10. The method as claimed in claim 1, wherein the process (9) comprises the steps of:
taking a raw material, wherein the raw material is annatto seeds;
crushing the raw material to a size in the range of 8mm - 10mm using a hammer mill;
carrying out an extraction of the raw material, wherein the extraction is carried out by using the raw material and 50% aqueous ethanol mixed in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and aqueous ethanol, repeating the extraction step one more time and combining the filtrates, and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
11. The method as claimed in claim 1, wherein the process (10) comprises the steps of:
taking a raw material, wherein the raw material is bio-fortified dried moringa leaves;
crushing the raw material to a size in the range of 8mm - 10mm using a hammer mill;
carrying out an extraction of the raw material, wherein the extraction is carried out by using the raw material and water mixed in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, repeating the extraction step one more time and combining the filtrates;
carrying out a membrane filtration of the supernatant, wherein the membrane filtration carried out by passing the filtrates through a membrane with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately, and concentrate the supernatant;
passing the filtrate through a column packed with ion exchange resin (Amberlite);
eluting the column with acidic water (5% organic lemon juice in water), and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
12. The method as claimed in claim 1, wherein the process (11) comprises the steps of:
taking a raw material, wherein the raw material is bio-fortified dried moringa leaves;
crushing the raw material to a size in the range of 8mm - 10mm using a hammer mill;
carrying out an extraction of the raw material, wherein the extraction is carried out by using the raw material and water containing 5% lemon juice mixed in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water containing 5% lemon juice, repeating the extraction step one more time and combining the filtrates;
carrying out a membrane filtration of the supernatant, wherein the membrane filtration carried out by passing the filtrates through a membrane with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60 psi), and a filtrate and supernatant is collected separately, and concentrate the supernatant;
passing the filtrate through a column packed with cation exchange resin (Amberlite);
eluting the column with acidic water (5% organic lemon juice in water), and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.

13. A multivitamin and mineral composition comprising:
a guava leaves extract powder type 1;
a guava leaves extract powder type 2;
a dried lemon peel extract powder type 1;
a dried lemon peel extract powder type 2;
a powder of amla fruit extract;
a powder of sesbania grandiflora leaves extract;
a powder of holy basil leaves extract;
a powder of annatto seeds extract type 1;
a powder of annatto seeds extract type 2;
a powder of moringa leaves extract type 1; and
a powder of moringa leaves extract type 2.
14. The composition as claimed in claim 11, wherein the composition is in the powdered form.
15. The composition as claimed in claim 11, wherein the composition comprises vitamin C, vitamin D, vitamin E, niacin, vitamin B6, vitamin B12, folic acid and biotin, and calcium, magnesium, iodine, potassium, copper, zinc, phosphorus, manganese, chromium and selenium.
16. The composition as claimed in claim 11, wherein the composition is effective in the treatment of diarrhea, Irritable Bowel Syndrome (IBS), antibiotic associated diarrhea (AAD) related to Clostridium difficile, Clostridium difficile associated diarrhoea (CDAD) and recurrent Clostridium difficile infections (RCDI).
17. The method as claimed in claim 1, wherein the dietary supplement further comprises dietary supplemental amounts of one or more compounds selected from vitamins and minerals other than calcium or phosphorus.
, Description:A) TECHNICAL FIELD OF INVENTION
[001] The present invention generally relates tomultivitamin and mineral supplement composition and particularly to a method of synthesizing enriched vitamins and minerals from biofortified raw materials. The present invention more particularly relates to a composition and a method of synthesizing natural vitamins and minerals extracted and enriched using agronomic biofortified crops for use in intestinal inflammation associated with dysbiosis of the gut microbiota.

B) BACKGROUND OF INVENTION
[002] The gut microbiota maintains a symbiotic relationship with the host and regulates several important functions including host metabolism, immunity, and intestinal barrier function. Intestinal inflammation is commonly associated with dysbiosis of the gut microbiota. Alterations in the gut microbiota and associated changes in metabolites as well as disruptions in the intestinal barrier are evidence of the relationship between the gut microbiota and intestinal inflammation. Recent studies have found that many factors may alter the gut microbiota, with the effects of diet being commonly studied.
[003] There are number of ways wherein an unhealthy gut might manifest itself. Some of the most common signs are upset stomach, high sugar diet, unintentional weight changes, sleep disturbances and constant fatigue, skin irritation, autoimmune conditions, food intolerances, etc. Diet is one of many important factors which can affect the gut microbiota and many other aspects of human health and disease. The gut microbiota uses ingested nutrients as an energy source in fundamental biological processes, and changes in diet type or pattern may change the composition of the gut microbiota, as species more suited to metabolizing novel diet types will increase in population, while other species may become less abundance. These changes in the composition of the gut microbiota may affect host physiology and disease resistance.
[004] Fortification is the practice of deliberately increasing the content of an essential micronutrient, i.e. vitamins and minerals (including trace elements) in a food, so as to improve the nutritional quality of the food supply and provide a public health benefit with minimal risk to health.Biofortification is the process by which the nutritional quality of food crops is improved through agronomic practices, conventional plant breeding, or modern biotechnology. Biofortification differs from conventional fortification in that biofortification aims to increase nutrient levels in crops during plant growth rather than through manual means during processing of the crops. Biofortification may therefore present a way to reach populations where supplementation and conventional fortification activities may be difficult to implement and/or limited.
[005] The agronomical techniques include the application of various abiotic elicitors, precursor molecules, pathway intermediates, zinc containing fertilizers, use of soil amendments like lime, gypsum, organic amendments like fym, application of bio-fertilizers like rhizobium (for legumes), azotobacter and azospirillum (non-leguminous crops) and azolla (water fern). Biofortification of curry leaves, holy basil leaves, guava fruits, guava leaves, annato seeds, lemon fruits, amla fruits, Sesbania grandiflora, and bamboo shoots are being employed commercially.
[006] However, there is still a need for a nutritional composition that is capable of promoting a well-balanced intestinal microbiota. There is a need to develop a composition and a method of synthesizing a composition or products that are enriched with essential vitamins and minerals which promote the growth of the gut microbiota and promote or restore an optimal intestinal microbiota ecosystem in an individual beneficial for it.
[007] The value additions and above mentioned shortcomings, disadvantages and problems are addressed herein, as detailed below.

C) OBJECT OF INVENTION
[008] The primary object of the present invention is to provide a multivitamin and mineral composition and a method of synthesizing a composition comprising essential vitamins and minerals from biofortified raw materials.
[009] Another object ofthe present invention is to provide a multivitamin and mineral composition used to promote the growth of the gut microbiota and to promote or restore an optimal intestinal microbiota ecosystem in an individual beneficial for it.
[0010] Yet another object of the present invention is to provide a multivitamin and mineral composition which is in the form of a powder.
[0011] Yet another object of the present invention is to provide a novel method of synthesizing the multivitamin and mineral composition.
[0012] These and other objects and advantages of the embodiments herein will become readily apparent from the following detailed description taken in conjunction with the accompanying drawings.

D) SUMMARY OF INVENTION
[0013] The various embodiments of the present invention provide amethod of synthesizing a multivitamin and mineral composition. The method comprisesmixing a guava leaves extract powder type 1, a guava leaves extract powder type 2, a dried lemon peel extract powder type 1, a dried lemon peel extract powder type 2, a powder of amla fruitextract, a powder of sesbania grandiflora leavesextract, a powder of holy basil leavesextract,a powder of annatto seeds extract type 1, a powder of annatto seeds extract type 2, a powder of moringa leaves extract type 1, and a powder of moringa leaves extract type 2,to obtain a mixture. The mixture ismilled and blended. The mixture is sifted through a 40 mesh sifter, and packed in an air tight container.
[0014] According to an embodiment of the present invention, the guava leaves extract powder type 1 is obtained by a process (1).
[0015] According to an embodiment of the present invention, theguava leavesextract powder type 2 is obtained by a process (2).
[0016] According to an embodiment of the present invention, thedried lemon peel extract powder type 1 is obtained by a process (3).
[0017] According to an embodiment of the present invention, thedried lemon peel extract powder type 2 is obtained by a process (4).
[0018] According to an embodiment of the present invention, thepowder of amla fruit extract is obtained by a process (5).
[0019] According to an embodiment of the present invention, thepowder of sesbania grandiflora leaves extract is obtained by a process (6).
[0020] According to an embodiment of the present invention, thepowder of holy basil leaves extract is obtained by a process (7).
[0021] According to an embodiment of the present invention, thepowder of annatto seedsextract type 1 is obtained by a process (8).
[0022] According to an embodiment of the present invention, thepowder of annatto seeds extract type 2 is obtained by a process (9).
[0023] According to an embodiment of the present invention,the powder of moringa leaves extract type 1 is obtained by a process (10).
[0024] According to an embodiment of the present invention,the powder of moringa leaves extract type 2 is obtained by a process (11).
[0025] According to an embodiment of the present invention, the process (1) comprises the steps of:
a. taking a raw material, wherein the raw material is bio-fortified dried guava leaves;
b. crushing the dried leaves to a size in the range of 8mm - 10mm;
c. carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water added in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
d. carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
e. concentrating the supernatant;
f. passing the filtrate through a column packed with ion exchange resin (Amberlite);
g. eluting the column with acidic water (5% organic lemon juice in water) and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
h. spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0026] According to an embodiment of the present invention, theprocess (2) comprises the steps of:
a. taking a raw material, wherein the raw material is bio-fortified dried guava leaves;
b. crushing the dried leaves to a size in the range of 8mm – 10mm;
c. carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water containing 5% lemon juice added in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
d. carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
e. passing the filtrate through a column packed with cation exchange resin;
f. eluting the column with acidic water (5% organic lemon juice in water) and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%; and
g. spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0027] According to an embodiment of the present invention, the process (3) comprises the steps of:
a. taking a raw material, wherein the raw material is bio-fortified dried lemon peel;
b. crushing the dried lemon peel to a size in the range of 8mm - 10mm using a hammer mill;
c. carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water added in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
d. carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
e. passing the filtrate through a column packed with ion exchange resin (Amberlite);
f. eluting the column with acidic water (5% organic lemon juice in water) and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
g. spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0028] According to an embodiment of the present invention,the process (4) comprises the steps of:
a. taking a raw material, wherein the raw material is bio-fortified dried lemon peel;
b. carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water containing 5% lemon juice added in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
c. carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
d. dissolving the filtrate from column elution in 50% ethanol;
e. passing the filtrate through a column packed with macro porous absorbent resin;
f. eluting the column with different percentage of ethanol, wherein different percentage of ethanol is in the range of 50-80%, and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%; and
g. spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0029] According to an embodiment of the present invention,theprocess (5) comprises the steps of:
a. taking a raw material, wherein the raw material is bio-fortified amla fruit;
b. crushing the raw material, squeezing the raw material and filtering the raw material to obtain a juice;
c. carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the juice through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
d. passing the filtrate through a column packed with poly-4-vinylpyridine ion exchange resin;
e. eluting the column with hot water in presence of 5% lemon water;
f. collecting the eluate and concentrating the eluate at 70°C to 80°C to obtain TDS of 30 to 40%;
g. spray drying the eluate at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0030] According to an embodiment of the present invention,theprocess (6) comprises the steps of:
a. taking a raw material, wherein the raw material is bio-fortified dried sesbania grandiflora leaves;
b. crushing the dried lemon peel to a size in the range of 8mm - 10mm using a hammer mill;
c. carrying out an extraction of the raw material, wherein the extraction is carried out by using the raw material and aqueous ethanol mixed in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and aqueous ethanol, repeating the extraction step one more time and combining the filtrates;
d. carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
e. passing the filtrate through a column packed with agarose polyaldehyde beads resin;
f. eluting the column with 9% lemon juice and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
g. spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0031] According to an embodiment of the present invention,theprocess (7) comprises the steps of:
a. taking a raw material, wherein the raw material is bio-fortified dried holy basil leaves;
b. crushing the raw material to a size in the range of 8mm - 10mm using a hammer mill;
c. carrying out an extraction of the raw material, wherein the extraction is carried out by using the raw material and aqueous ethanol mixed in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and aqueous ethanol, repeating the extraction step one more time and combining the filtrates, and concentrate to 4:1 ratio;
d. adding 50% ethanol and keeping the concentrate over night, and collecting a supernatant;
e. carrying out a membrane filtration of the supernatant, wherein the membrane filtration carried out by passing the supernatant through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately, and concentrate the supernatant;
f. passing the filtrate through a column packed with ion exchange resin (Amberlite);
g. eluting the column with acidic water (5% organic lemon juice in water), and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
h. spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0032] According to an embodiment of the present invention,theprocess (8) comprises the steps of:
a. taking a raw material, wherein the raw material is annatto seeds;
b. crushing the raw material to a size in the range of 8mm - 10mm using a hammer mill;
c. carrying out an extraction of the raw material, wherein the extraction is carried out by using the raw material and aqueous ethanol mixed in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and aqueous ethanol, repeating the extraction step one more time and combining the filtrates;
d. carrying out a membrane filtration of the supernatant, wherein the membrane filtration carried out by passing the supernatant through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately, and concentrate the supernatant;
e. passing the filtrate through a column packed with non polar polystyrene adsorbent (SM-2 resin);
f. eluting the column with ethanol, and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
g. spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0033] According to an embodiment of the present invention,the process (9) comprises the steps of:
a. taking a raw material, wherein the raw material is annatto seeds;
b. crushing the raw material to a size in the range of 8mm - 10mm using a hammer mill;
c. carrying out an extraction of the raw material, wherein the extraction is carried out by using the raw material and 50% aqueous ethanol mixed in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and aqueous ethanol, repeating the extraction step one more time and combining the filtrates, and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
d. spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0034] According to an embodiment of the present invention, the process (10) comprises the steps of:
a. taking a raw material, wherein the raw material is bio-fortified dried moringa leaves;
b. crushing the raw material to a size in the range of 8mm - 10mm using a hammer mill;
c. carrying out an extraction of the raw material, wherein the extraction is carried out by using the raw material and water mixed in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, repeating the extraction step one more time and combining the filtrates;
d. carrying out a membrane filtration of the supernatant, wherein the membrane filtration carried out by passing the filtrates through a membrane with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately, and concentrate the supernatant;
e. passing the filtrate through a column packed with ion exchange resin (Amberlite);
f. eluting the column with acidic water (5% organic lemon juice in water), and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
g. spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0035] According to an embodiment of the present invention,the process (11) comprises the steps of:
a. taking a raw material, wherein the raw material is bio-fortified dried moringa leaves;
b. crushing the raw material to a size in the range of 8mm - 10mm using a hammer mill;
c. carrying out an extraction of the raw material, wherein the extraction is carried out by using the raw material and watercontaining 5% lemon juice mixed in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and watercontaining 5% lemon juice, repeating the extraction step one more time and combining the filtrates;
d. carrying out a membrane filtration of the supernatant, wherein the membrane filtration carried out by passing the filtrates through a membrane with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately, and concentrate the supernatant;
e. passing the filtrate through a column packed with cation exchange resin (Amberlite);
f. eluting the column with acidic water (5% organic lemon juice in water), and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
g. spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0036] According to an embodiment of the present invention,a multivitamin and mineral composition comprises a guava leaves extract powder type 1, a guava leaves extract powder type 2, a dried lemon peel extract powder type 1, a dried lemon peel extract powder type 2, a powder of amla fruitextract, a powder of sesbania grandiflora leavesextract, a powder of holy basil leavesextract, a powder of annatto seeds extract type 1; anda powder of annatto seedsextract type 2, a powder of moringa leaves extract type 1, and a powder of moringa leaves extract type 2.
[0037] According to an embodiment of the present invention,the composition is in the powdered form.
[0038] According to an embodiment of the present invention,the composition comprises vitamin C, vitamin D, vitamin E, niacin, vitamin B6, vitamin B12, folic acid and biotin, and calcium, magnesium, iodine, potassium, copper, zinc, phosphorus, manganese, chromium and selenium.
[0039] According to an embodiment of the present invention, the composition is effective in the treatment of diarrhoea, Irritable Bowel Syndrome (IBS), antibiotic associated diarrhoea (AAD) related to Clostridium difficile, Clostridium difficile associated diarrhoea (CDAD) and recurrent Clostridium difficile infections (RCDI).
[0040] According to an embodiment of the present invention, the dietary supplement further comprises dietary supplemental amounts of one or more compounds selected from vitamins and minerals other than calcium or phosphorus.
[0041] These and other aspects of the embodiments herein will be better appreciated and understood when considered in conjunction with the following description and the accompanying drawings. It should be understood, however, that the following descriptions, while indicating preferred embodiments and numerous specific details thereof, are given by way of illustration and not of limitation. Many changes and modifications may be made within the scope of the embodiments herein without departing from the spirit thereof, and the embodiments herein include all such modifications.

E) BRIEF DESCRIPTION OF DRAWINGS
[0042] The other objects, features and advantages will occur to those skilled in the art from the following description of the preferred embodiment and the accompanying drawings in which:
[0043] FIG. 1 is a flowchart showing the steps involved in the method of synthesizing a multivitamin and mineral composition, according to an embodiment of the present invention.

F) DETAILED DESCRIPTION OF DRAWINGS
[0044] In the following detailed description, a reference is made to the accompanying drawings that form a part hereof, and in which the specific embodiments that may be practiced is shown by way of illustration. The embodiments are described in sufficient detail to enable those skilled in the art to practice the embodiments and it is to be understood that the logical, mechanical, electronic and other changes may be made without departing from the scope of the embodiments. The following detailed description is therefore not to be taken in a limiting sense.
[0045] The various embodiments of the presentprovide a multivitamin and mineral compositioncomprising natural vitamins and minerals extracted and enriched using agronomic biofortified crops. The composition is used is situation of intestinal inflammation associated with dysbiosis of the gut microbiota.
[0046] FIG. 1 is a flowchart showing the steps involved in the method of synthesizing a multivitamin and mineral composition, according to an embodiment of the present invention. With respect of FIG. 1, the method comprises mixing a guava leaves extractpowder type 1, a guava leaves extractpowder type 2, a dried lemon peel extractpowder type 1, a dried lemon peel extractpowder type 2, a powder of amla fruitextract, a powder of sesbania grandiflora leavesextract, a powder of holy basil leavesextract, a powder of annatto seeds extracttype 1, a powder of annatto seeds extracttype 2, powder of moringa leaves extract type 1, and a powder of moringa leaves extract type 2 to obtain a mixture(101). The mixture is milled and blended(102). The mixture is sifted through a 40 mesh sifter(103), and packed in an air tight container(104).
[0047] According to an embodiment of the present invention, the guava leaves powder extracttype 1 is obtained by a process (1).
[0048] According to an embodiment of the present invention, the guava leaves powder extracttype 2 is obtained by a process (2).
[0049] According to an embodiment of the present invention, the dried lemon peel powderextract type 1 is obtained by a process (3).
[0050] According to an embodiment of the present invention, the dried lemon peel powder extracttype 2 is obtained by a process (4).
[0051] According to an embodiment of the present invention, the powder of amla fruit extractis obtained by a process (5).
[0052] According to an embodiment of the present invention, the powder of sesbania grandiflora leaves extractis obtained by a process (6).
[0053] According to an embodiment of the present invention, the powder of holy basil leaves extractis obtained by a process (7).
[0054] According to an embodiment of the present invention, the powder of annatto seedsextract type 1 is obtained by a process (8).
[0055] According to an embodiment of the present invention, the powder of annatto seeds extracttype 2 is obtained by a process (9).
[0056] According to an embodiment of the present invention, the powder of moringa leaves extract type 1 is obtained by process (10).
[0057] According to an embodiment of the present invention, the powder of moringa leaves extract type 2 is obtained by process (11).
[0058] According to an embodiment of the present invention, the raw materials comprises mango, curry leaves, holy basil leaves, guava fruits, guava leaves, annato seeds, lemon fruits, amla fruits, sesbania grandiflora, bamboo shoots, mustard seed, lantana camara and moringa leaves.
[0059] According to an embodiment of the present invention, the composition is a dietary supplement.
[0060] According to an embodiment of the present invention, the composition comprises a combination of vitamins and minerals.
[0061] According to an embodiment of the present invention, the vitamins are vitamin C, vitamin D, vitamin E, niacin, vitamin B6, vitamin B12, folic acid and biotin.
[0062] According to an embodiment of the present invention, the minerals are calcium, magnesium, iodine, potassium, copper, zinc, phosphorus, manganese, chromium and selenium.
[0063] According to an embodiment of the present invention, the multivitamin and mineral composition is a fortifying beverage or food product with one or more vitamins and minerals, said vitamin composition comprises a powder containing one or more vitamins and minerals.
[0064] According to an embodiment of the present invention, a multi-vitamin and mineral supplement composition is provided for a lactating woman comprising vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B7, vitamin B9 and minerals comprising calcium, magnesium, iodine, potassium, copper, zinc, phosphorus, manganese, chromium and selenium.
[0065] According to an embodiment of the present invention, a multi-vitamin and mineral composition is a daily dietary multivitamin and mineral supplement comprisingbioflavonoids.
PROCESS AND PROCEDURE IN DETAIL
[0066] Guava fruit and leaves extracts powder type 1 (Process 1):
[0067] Raw material: Bio-fortified dried guava leaves free from foreign material are selected.
[0068] Crushing: Crush the dried leaves to reduce the size to 8mm - 10mm using hammer mill.
[0069] Extraction: Charge the raw material and water in the ratio 1:3, into the extractor. Raise the temperature to 70°C to 80°C and circulate the water for 3 hours. After 3 hour filter the extract and collect the filtrate.
[0070] Repeat the above extraction step for one more time. Combine both the filtrates.
[0071] Membrane filtration: Pass the filtrates through membrane with pore size 0.03 to 10 microns, with feed operating pressure approximately 100 to 400kPa (15 to 60psi). Collect both the filtrate and supernatant separately. Concentrate the supernatant.
[0072] Column elution/separation: Pass the filtrate through the column packed with ion exchange resin (Amberlite). Elute the column with acidic water (5% organic lemon juice in water). Collect the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[0073] Spray drying: Spray dry the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.

[0074] Guava fruit and leaves extracts powder type 2 (process 2):
[0075] Raw material: Bio-fortified dried guava leaves free from foreign material are selected.
[0076] Crushing: Crush the dried leaves to reduce the size to 8mm - 10mm using hammer mill.
[0077] Extraction: Charge the raw material and water containing 5% lemon juice in the ratio 1:3, into the extractor. Raise the temperature to 70°C to 80°C and circulate the water for 3 hours. After 3 hour filter the extract and collect the filtrate.
[0078] Repeat the above extraction step for one more time. Combine both the filtrates.
[0079] Membrane filtration: Pass the filtrates through membrane with pore size 0.03 to 10 microns, with feed operating pressure approximately 100 to 400kPa (15 to 60psi). Collect both the filtrate and supernatant separately.
[0080] Column elution/separation : Pass the filtrate through the column packed with cation exchange resin. Elute the column with acidic water (5% organic lemon juice in water).
[0081] Collect the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[0082] Spray drying: Spray dry the concentrated aqueous solution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.

[0083] Dried lemon peel extract powder type 1 (Process 3):
[0084] Raw material: Biofortified dried lemon peel free from foreign material is selected.
[0085] Crushing: Crush the dried lemon peel to reduce the size to 8mm - 10mm using hammer mill.
[0086] Extraction: Charge the raw material and water in the ratio 1:3, into the extractor. Raise the temperature to 70°C to 80°C and circulate the water for 3 hours. After 3 hour filter the extract and collect the filtrate.
[0087] Repeat the above extraction step for one more time. Combine both the filtrates.
[0088] Membrane filtration: Pass the filtrates through membrane with pore size 0.03 to 10 microns, with feed operating pressure approximately 100 to 400kPa (15 to 60psi). Collect both the filtrate and supernatant separately. Concentrate the supernatant.
[0089] Column elution/separation: Pass the filtrate through the column packed with ion exchange resin (Amberlite). Elute the column with acidic water (5% organic lemon juice in water). Collect the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[0090] Spray drying: Spray dry the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.

[0091] Dried lemon peel extract powder type 2 (Process 4):
[0092] Raw material: Bio-fortified dried lemon peel free from foreign material are selected.
[0093] Extraction: Charge the raw material and water 1:3, into the extractor. Raise the temperature to 70°C to 80°C and circulate the water for 3 hours. After 3 hour filter the extract and collect the filtrate.
[0094] Repeat the above extraction step for one more time. Combine both the filtrates.
[0095] Membrane filtration: Pass the filtrates through membrane with pore size 0.03 to 10 microns, with feed operating pressure approximately 100 to 400kPa (15 to 60psi). Collect both the Filtrate and supernatant separately. Concentrate the supernatant.
[0096] Column elution/separation : Dissolve the filtrate from column elution in 50% ethanol and pass the filtrate through the column packed with macro porous adsorbent resin . Elute the column using different percentage of ethanol (50-80%) in a gradient manner. Collect the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[0097] Spray drying: Spray dry the concentrated aqueous solutions of supernatant and filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.

[0098] Powder of amla fruit extract (Process 5):
[0099] Raw material: Bio-fortified amla fruit free from foreign material are selected.
[00100] Crushing and squeezing: Crush the amla fruit, squeeze the crushed amla fruit and filter to obtain amla juice.
[00101] Membrane filtration: Pass the filtrates through membrane with pore size 0.03 to 10 microns, with feed operating pressure approximately 100 to 400kPa (15 to 60psi). Collect both the
[00102] Filtrate and supernatant separately.
[00103] Column elution/separation : Pass the filtrate through the column packed with poly 4 vinylpyridine ion exchange resin. Elute the column with hot water (75°C) in presence of 5% organic lemon juice. Collect the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[00104] Spray drying: Spray dry the concentrated aqueous solution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.

[00105] Powder of sesbania grandiflora leaves extract (Process 6):
[00106] Raw material: Biofortified dried sesbania grandiflora leaves free from foreign material are selected.
[00107] Crushing: Crush the dried leaves to reduce the size to 8mm - 10mm using hammer mill.
[00108] Extraction: Charge the raw material and aqueous ethanol in the ratio 1:3, intothe extractor. Raise the temperature to 70°C to 80°C and circulate the water for 3 hours. After 3 hours filter the extract and collect the filtrate.
[00109] Repeat the above extraction step for one more time. Combine both the filtrates.
[00110] The filtrates obtained from extraction will undergo two types of process:
[00111] Process I:Membrane filtration: Pass the filtrates through membrane with pore size 0.03 to 10 microns, with feed operating pressure approximately 100 to 400kPa (15 to 60psi). Collect both Filtrate and supernatant separately. Concentrate the supernatant.
[00112] Column elution/separation: Pass the filtrate through the column packed with agarose polyaldehyde beads (resin). Elute the column with 9% lemon juice. Collect the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[00113] Spray drying: Spray dry the concentrated solution from column elution and concentrated supernatant from membrane filtration at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.
[00114] Process II: Concentration: Concentrate the filtrates obtained from extraction.
[00115] Acidification: precipitate the filtrate by acidification using lemon juice.
[00116] Settling: After acidification, allow the filtrate for settling overnight.
[00117] Filtration: Filter the above solution and collect the supernatant.
[00118] Spray dry : Spray dry the solution at inlet temperature 180° to 190°C and outlettemperature 80°C to 100°C. Collect the dried powder.

[00119] Powder of holy basil leaves extract (Process 7)
[00120] Raw material: Bio-fortified dried holy basil leaves from foreign material are selected.
[00121] Crushing: Crush the holy basil leaves to reduce the size to 8mm - 10mm using hammer mill.
[00122] Extraction: Charge the raw material and water in the ratio 1:3, into the extractor. Raise the temperature to 70°C to 80°C and circulate the water for 3 hours. After 3 hour filter the extract and collect the filtrate.
[00123] Repeat the above extraction step for one more time. Combine both the filtrates and concentrate to 4:1 ratio.The concentrate ratio ¼ of initial volume.
[00124] Settling : Add 50% ethanol to the concentrate and keep for settling over night. Clear supernatant obtained is subjected to membrane filtration.
[00125] Membrane filtration: Pass the clear supernatant through membrane with pore size 0.03 to10 microns, with feed operating pressure approximately 100 to 400kPa (15 to 60psi). Collect both the filtrate and supernatant separately. Concentrate the supernatant.
[00126] Column elution/separation : Pass the filtrate through the column packed with ion exchange resin (Amberlite) . Elute the column with acidic water (5% organic lemon juice in water).Collect the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[00127] Spray drying: Spray dry the concentrated aqueous solution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.

[00128] Powder of annatto seeds and fruit extracts type 1 (Process 8)
[00129] Raw material: Annatto seed free from foreign material are selected.
[00130] Crushing: Crush the dried leaves to reduce the size to 8mm - 10mm using hammer mill.
[00131] Extraction: Charge the raw material and water in the ratio 1:3, into the extractor. Raise the temperature to 70°C to 80°C and circulate the water for 3 hours. After 3 hour filter the extract and collect the filtrate.
[00132] Repeat the above extraction step for one more time. Combine both the filtrates.
[00133] Membrane filtration: Pass the filtrates through membrane with pore size 0.03 to 10 microns, with feed operating pressure approximately 100 to 400kPa (15 to 60psi). Collect both Filtrate and supernatant separately. Concentrate the supernatant.
[00134] Column elution/separation : Pass the filtrate through the column packed with non polar polystyrene adsorbent (SM-2 resin). Elute the column with Ethanol. Collect the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[00135] Spray drying: Spray dry the concentrated solution from column elution and concentrated supernatant from membrane filtration at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.

[00136] Powder of annatto seeds and fruit extracts type 2 (Process 9):
[00137] Raw material: Annatto seeds free from foreign material are selected.
[00138] Crushing: Crush the annatto seeds to reduce the size to 8mm - 10mm using hammer mill.
[00139] Extraction: Charge the raw material and 50% aqueous ethanol intheratio 1:3, into the extractor. Raise the temperature to 70°C to 80°C and circulate the water for 3 hours. After 3 hour filter the extract and collect the filtrate.
[00140] Repeat the above extraction step for one more time. Combine both the filtrates, and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[00141] Spray drying: Spray dry the concentrated aqueous solution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.
[00142] Powder of moringa leaves extract type 1 (Process 10)
[00143] Raw material: Bio-fortified dried moringa leaves free from foreign material are selected.
[00144] Crushing: Crush the dried leaves to reduce the size to 8mm - 10mm using hammer mill.
[00145] Extraction: Charge the raw material and water in the ratio 1:3, into the extractor. Raise the temperature to 70°C to 80°C and circulate the water for 3 hours. After 3 hour filter the extract and collect the filtrate.
[00146] Repeat the above extraction step for one more time. Combine both the filtrates.
[00147] Membrane filtration: Pass the filtrates through membrane with pore size 0.03 to 10 microns, with feed operating pressure approximately 100 to 400kPa (15 to 60psi). Collect both the filtrate and supernatant separately. Concentrate the supernatant.
[00148] Column elution/separation: Pass the filtrate through the column packed with ion exchange resin (Amberlite). Elute the column with acidic water (5% organic lemon juice in water). Collect the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[00149] Spray drying: Spray dry the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.
[00150] Powder of moringa leaves extract type 2 (Process 11)
[00151] Raw material: Bio-fortified dried moringa leaves free from foreign material are selected.
[00152] Crushing: Crush the dried leaves to reduce the size to 8mm - 10mm using hammer mill.
[00153] Extraction: Charge the raw material and water containing 5% lemon juice in the ratio 1:3, into the extractor. Raise the temperature to 70°C to 80°C and circulate the water for 3 hours. After 3 hour filter the extract and collect the filtrate.
[00154] Repeat the above extraction step for one more time. Combine both the filtrates.
[00155] Membrane filtration: Pass the filtrates through membrane with pore size 0.03 to 10 microns, with feed operating pressure approximately 100 to 400kPa (15 to 60psi). Collect both the filtrate and supernatant separately.
[00156] Column elution/separation : Pass the filtrate through the column packed with cation exchange resin. Elute the column with acidic water (5% organic lemon juice in water).
[00157] Collect the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[00158] Spray drying: Spray dry the concentrated aqueous solution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.
[00159] PACKING : Combine dried powder from process 1, process 2, process 3, process 4, process 5, process 6, process 7,process 8 and process 8 as desired are milled, blended an hour, sifted through 40mesh and packed in a air tight container.
[00160] According to an embodiment of the present invention, a multivitamin and mineral composition comprising the ingredients as stated in Table 1. The composition is in powdered form.
Table 1: Multivitamin and mineral composition
Guava leaves and fruit extracts
Process -1
Process -2 0.025-0.5kg
5.0 – 7.0kg
Lemon peel extract
Process -3
Process -4 0.8-1.2kg

25-30 kg
Amla extract
Process -5
20-25kg
Sesbania extract
Process -6
0.020-0.045
Holy basil extract
Process -7
25-30kg
Annatto seed and fruit extracts
Process -8
Process -9 1-1.5kg
4-5kg
Moringa leaves extract Process – 10
Process - 11
5-10kg
[00161] According to an embodiment of the present invention, the composition is effective in the treatment of diarrhoea, Irritable Bowel Syndrome (IBS), antibiotic associated diarrhoea (AAD) related to Clostridium difficile, Clostridium difficile associated diarrhoea (CDAD) and recurrent Clostridium difficile infections (RCDI).
[00162] According to an embodiment of the present invention, the dietary supplement further comprises dietary supplemental amounts of one or more compounds selected from vitamins and minerals other than calcium or phosphorus.

G) ADVANTAGES OF INVENTION
[00163] The present invention addresses the problem of gut dysbiosis wherein the composition of the present invention assists in promoting a gut lining and tight junction functioning, ultimately leading to associated effects like reduced inflammation in the intestinal lining.
[00164] The vitamins and minerals composition is used for improving the condition of the digestive system by maintaining a healthy microbiota population by mending ulcerated and damaged intestinal epithelial cell surfaces for the microbiota to populate.
[00165] The vitamins and minerals composition supplies the right amount of the right micronutrients at the right time to assure adequate intake of micronutrients needed for disease prevention and protection against nutritional losses and deficiencies due to lifestyle factors and common inadequate dietary patterns.
[00166] It is to be understood that the phraseology or terminology employed herein is for the purpose of description and not of limitation. Therefore, while the embodiments herein have been described in terms of preferred embodiments, those skilled in the art will recognize that the embodiments herein can be practiced with modification within the spirit and scope of the claims presented in the complete specification or non-provisional application.