FORM 2
The Patents Act,1970,
{39 of 1970)
&
The Patents Rule, 2003
Complete Specification
(See Section 10 rule 13),
Rashtriya Chemicals and Fertilizers ltd.
(A Government of India Undertaking)
Research and Development- Bioresearch
Mumbai- 400074
'Nano Nutrient composition ' Indian Company

The field of invention;
The field of invention relates to a fertilizer composition for supplying nutrient to crops and also provides soil conditioning; more particularly invention relates to nano nutrient fertilizer composition comprising soil conditioning agents, extract of sea weed, plant growth stimulant, micronutrients in the nano form;
Background of invention and prior art;
Fertilizer is nothing but natural or artificial substance containing the chemical elements that improve growth and productiveness of plants. Fertilizers enhance the natural fertility of the soil or replace the chemical elements taken from the soil by previous crops.
Fertilizer can be in granular form in combination with natural organic ingredients and nanoparticles, enhances the yield of the crop, maintains the soil fertility and health, enhances the microbial growth in soil and increases the soil health index. The product shows effects with recommended dose of fertilizers and also with reduce fertilizer dosages. The nano nutrient composition consists of a granular form useful for soil application. The soil application also acts as a soil amending substance, improving
the soil physico-chemical and biological properties.
»... U.S. Patent 4008065 discloses granulated fertilizer composition fertilizer adjuvant selected from the
group consisting of sulfite waste liquor, starch, molasses, sugar, water glass, gypsum, clay, natural or
synthetic water-soluble polymers and mixtures thereof.
U.S.Patent 5,840,656 teaches combinations of fertilizer with certain organic compounds to increase fertilizer efficiency, plant productivity, growth, and nutrient accumulation. These beneficial effects are accomplished using combinations of a fertilizer and an amino acid selected from .gamma,-aminobutyric acid, glutamic acid, and a mixture of .gamma.-aminobutyric acid and glutamic acid. A source of proteinaceous amino acids and a carbon skeleton may also be used with the fertilizer and the amino acid. The specification describes compositions and methods employing such combinations
to take advantage of their beneficial effects,
* -.
US Patent No 8486944B2 discloses a composition to enhance efficiency of Photosynthesis and thereby increase agriculture/horticulture yield, containing approximately 5% to 90% Melamine, approximately 10% to 80% Ortho Phosphoric acid, approximately 8 Sea Weed Extract;

Therefore, in the present application it is an object to provide an improved fertilizer composition having properties to improve soil condition better yield, as well as to provide crop nutritional properties,
Object of the present invention;
It is an object of the present invention to provide fertilizer composition having properties to improve soil condition better yield, as well as to provide crop nutritional properties,
It is also an object of the present invention to provide a fertilizer composition having soil conditioning agents to improve soil quality;
It is also an object of the present invention to provide a fertilizer composition for crop nutrition for appropriate growth having biostimulants; plant growth agents, micro-nutrients in the form of nanoparticles;
Summary of the invention;
In one of the aspect of the invention it is provided that a fertilizer composition having soil conditioner or optionally mixed with organic fertilizer such as vermicompost along with biostimulants, sea weed extract, micronutrient in the form of nanoparticles is mixed to form fertilizer composition;
Detailed description of the invention;
In one of the important aspect of the invention fertilizer composition having provide fertilizer composition having properties to improve soil condition better yield, as well as to provide crop nutritional properties,
The fertilizer composition is having soil conditioning agents, biostimulants, organic matter, micronutrient in the form of nanoparticles;
The soil conditioning agents due to their ability to improve soil quality, inorganic soil amendments
'■■.. include lime and gypsum, in particular gypsum is used in present invention;

Gypsum provides an advantage for improving soil quality due to Gypsum to Improve Soil Chemical Properties the detrimental effects on plant growth of subsoil acidity, particularly at high levels of
exchangeable aluminum (AI3+), are well known. The lower the soil pH, the greater the concentration
«...
of soluble and available aluminum. For many plants growing in acid soils, it is not the pH that is especially toxic, this can greatly increase the supply of water and nutrients to crops. This is especially important in the dry season of arid areas, and a positive response to FGD gypsum is often greatest in moisture stress conditions.
In the other aspect of the invention it is provided that the bio-stimulant is added in the fertilizer * composition for the growth of crops, the bio-stimulant used in the present invention is Plant-derived protein hydrolysates (PHs) have gained prominence as plant biostimulants because of their potential to increase the germination, productivity and quality of a wide range of horticultural and agronomic crops. Application of PHs can also alleviate the negative effects of abiotic plant stress due to salinity, drought and heavy metals. Recent studies aimed at uncovering the mechanisms regulating these beneficial effects indicate that PHs could be directly affecting plants by stimulating carbon and nitrogen metabolism, and interfering with hormonal activity. Indirect effects could also play a role as PHs could enhance nutrient availability in plant growth substrates, and increase nutrient uptake and nutrient-use efficiency in plants. Moreover, the beneficial effects of PHs also could be due to the stimulation of plant microbiomes. Plants are colonized by an abundant and diverse assortment of microbial taxa that can help plants acquire nutrients and water and withstand biotic and abiotic stress. The substrates provided by PHs, such as amino acids, could provide an ideal food source for these plant-associated microbes. Indeed, recent studies have provided evidence that plant microbiomes are modified by the application of PHs, supporting the hypothesis that PHs might be acting, at least in part, via changes in the composition and activity of these microbial communities. Application of PHs has great potential to meet the twin challenges of a feeding a growing population while minimizing agriculture's impact on human health and the environment. However, to fully realize the potential of PHs;
In an another aspect of the invention it is provided that soil conditioning agent is mixed with the vermin-compost, as vermin-compost is natural organic fertilizer , which enhances plant growth, suppresses disease in plants, increases porosity and microbial activity in soil, and improves water retention and aeration. Vermicompost

In an another aspect of the invention the fertilizer composition is having Sea weed extract as important ingredient, it is known as Seaweed extracts act as biostimulants mainly due to the presence of plant hormones, in the present invention Ascophyllum species including Ascophyllum nodosum;
Ascophyllum nodosum Species;
Ascophyllum nodosum is commonly known as rockweed, and is abundantly distributed throughout the northwest coast of Europe and the northeastern coast of North America reviewed the unique characteristics of A. nodosum as a prominent source for the production and synthesis of biostimulants. One unique feature of A. nodosum is its mutualistic association with the fungal endophyte Mycosphaerella ascophylli A. nodosum from desiccation;
ANE (Ascophyllum nodosum extract) Improves Salinity Tolerance in Plants,
Soil salinity is a global problem, affecting over 800 million hectares of land, resulting in massive impacts on agricultural productivity , Mild salinity stress causes physiological drought in plants, impairing cell-water relations, inhibiting cell expansion, and, consequently, reducing growth rate . Long-term exposure to high salinity causes ionic stress by disturbing the homeostasis of intracellular ions, which results in membrane dysfunction and attenuation of metabolic activity and secondary effects, inhibiting growth, and inducing cell death. Salinity induces both ionic and osmotic stresses, thus reducing plant growth and productivity. Plants have developed strategies to adapt to salinity stress at molecular, biochemical, and physiological levels.
ANE improves plant defences against various pathogens; Changing climatic conditions and intensive agricultural practices increase the emergence of infectious plant diseases, causing a reduction in agricultural productivity. Plant diseases are caused by pathogens such as bacteria, fungi, and viruses that disrupt plant health as well as their productivity. Plants have evolved several inducible defense mechanisms in order to deter these pathogens following infection . Two types of disease resistance mechanisms in plants have been reported: systemic acquired resistance (SAR) and induced systemic resistance(ISR): In SAR, SA plays a crucial role of mediating pathogenesis-related (PR) gene activation, while in ISR, JA; and ethylene (ET) pathways are important for the induction of broad-spectrum disease resistance;
ANE Improves Soil Health
Soil health, alternatively known as soil quality, is simply defined as: "the continued capacity of soil to function as a vital living ecosystem that sustains plants, animals and humans". A healthy soil

contributes to environmental management within the biosphere (air, water, and soil) and the productivity of plants and animals under both natural and managed systems they are chelators, directly contributing to the health of the soil. ANE provides natural chelation in the soil due to the presence of residual alginates present in the hydrolyzed extract, which allows for an increase in plant-available minerals and increased soil aeration and water-holding capacity
In the other aspect of the invention the fertilizer composition further having ZnO nano-particles as a micro-nutrient, it is evident that the ZnO nano-particles is having important role in plant physiology, as ZnO Zinc (Zn) is considered an essential micronutrient and its deficiency is an important health problem around the world and is a hurdle in achieving production targets in crops,
Among NPs, zinc oxides (ZnO) NPs are their potential adverse as well as positive effects on the ecosystem have been reported. The soil application of ZnO NPs (<100 mg kg-1) increased the Zn uptake by cucumber than bulk counterparts but negatively affected the growth of the plants at higher NPs (1000 mg kg-1).
Similarly, different methods are used to apply the ZnO nano particle on wheat plant, like foliar spray, soil mixing method and seed priming method. Zinc uptake by plants from NPs depends upon the soil properties which may restrict the nutrient uptake by plants due to variation in dissolution, aggregation, and change in surface properties of NPs in the soil. Seed priming method is simple and cost effective and primed seeds can rapidly absorb and renovate the seed metabolism, resulting in a higher germination rate and decreases intrinsic physiological non uniformity in germination. Seed priming method can also improve the growth quality and production of crops;
In the most important aspect of the invention it is provided that fertilizer composition of the present invention is having the strength of the ingredients in the following range,
Gypsum: 100-500 gm
Protein hydrolysate: 0.01-150 gm
Sea weed extract: 0.05-125 gm Ascophyllum species (purchase overseas)
Nanoparticles (Zno) up to 0.001-5 gm
The following is the most preferred composition;
In an another aspect of the present invention it is evident that the T3 , which is the fertilizer composition of the present invention is having the maximum growth in an experimental studies, it is found in the okra plant the growth of the maximum is achieved by using T3 composition,

In the other aspect of the invention it is provided with a process for manufacture of composition of the present invention, the process is as follows,
Each of the.500 grams of the composition;
Finely powdered Gypsum or gypsum plus vermicompost - 362.5 gm; Sea weed extract - 62.5 gm; Protein hydrolysate- 75 gm; Zinc oxide nano-particles- 2-5 gm;
Protein hydrolysate to be mixed in 80 ml water, this is to be poured from top of the granulator. rest of the material is added to the granulator. The impeller RPM is 55 for 2 min; Chopper RPM is 85 for 1 min;
The experimental results of the fertilizer composition of the present invention evident that the advantage over the other existing fertilizer composition as shown below,
Okra Harveting data (field no.01) Table 1;

Dose Total Weight (gm)
Tl 3284.90
T2 2848.80
T3 3754.45
T4 3002.17
T5 3104.60
T6 2982.75
T7 2779.99
T8 3160.61
T9 3186.08

Examples '
1. Preparation of the fertilizer composition;
Each of the 500 grams of the composition is prepared by adding finely powdered Gypsum 362.5 gm to Sea weed extract 62.5 gm by mixing continuously then adding Protein hydrolysate in 80 ml water, this is to be poured from top of the granulator rest of the material is added to the granulator.
With constant rotating in an impeller RPM is 55 for 2 min; and blending with chopper RPM is 85 for 1 min;
2. Preparation of the fertilizer composition;
Each of the 500 grams of the composition is prepared by adding finely powdered Gypsum vermicompost mix 362.5 gm to Sea weed extract 62.5 gm by mixing continuously then adding Protein hydrolysate in 80 ml water, this is to be poured from top of the granulator rest of the material is added to the granulator.
With constant rotating in an impeller RPM is 55 for 2 min; and blending with chopper RPM is 85 for 1 min;

Claims
1. Fertilizer composition comprises soil conditioner, bio-stimulant, sea weed extract, micro-nutriment in the form of nanoparticles, wherein soil conditioner is optionally mixed with organic fertilizer;
2. Fertilizer composition comprises according to claim 1 wherein soil conditioner is gypsum;

3. Fertilizer composition comprises according to claim 1 wherein biostimulant agent is protein hydrolysate;
4. Fertilizer composition comprises according to claim 1 wherein sea weed extract is ascophyllum species,
5. Fertilizer composition comprises according to claim 1 wherein micronutrient is ZnO,
6. Fertilizer composition comprises according to claim 1 wherein the quantity
Gypsum: or gypsum + vermicompost 100-500 gm
Protein hydrolysate: 0.01-150 gm
Sea weed extract: 0.05-125 gm ascophyllum species (purchase overseas.)
Nanoparticles (Zno) up to 0.001-5 gm
For 500 grams of the fertilizer composition;
7. Fertilizer composition comprises according to claim 1 wherein ascophyllum species is Ascophyllum nodosum Species;
8. Fertilizer composition comprises according to claim 1 wherein organic fertilizer is vermicompost;
9. A process for preparation of Fertilizer composition comprises
(i) adding finely powdered gypsum or Gypsum vermicompost combination mix 362.5 gm to Sea weed extract 62.5 gm by
(ii) mixing continuously then adding Protein hydrolysate in 80 ml water,
*-.
(iii) pouring from top of the granulator rest of the material is added to the granulator.
(iiii) constant rotating in an impeller RPM is 55 for 2 min; and blending with chopper RPM is 85 for 1min;