FIELD OF THE INVENTION
In June 1999, Richard Smalley, Nobel laureate in chemistry, addressed the US House Committee on Science on the benefits of nanotechnology. "The impact of nanotechnology on the health, wealth, and lives of people," he said, "will be at least the equivalent of the combined influences of microelectronics, medical imaging, computer-aided engineering and man-made polymers developed in this century."
Current nanotechnology has developed many different kinds of materials in a nano-sized material. When examined their physical and biological activity, there was significant difference in the material characters of the nano-sized materials. Recent advances in the field of nanotechnology, particularly the ability to prepare highly ordered nano particulates of any size and shape, have led to the development of new biocidal agents. Nano-materials are called "a wonder of modern medicine".
Nano particles have various functions that are not observed in bulk phase and have been studied extensively because of their exclusive catalytic, optical, electronic, magnetic and antimicrobial wound healing , antioxidant and anti-inflammatory properties . Over the past few decades, nano encapsulated vitamin, whose structures exhibit significantly novel and improved physical, chemical, and biological properties and functionality due to their nano-scale size, have elicited much interest.
OBJECT OF INVENTION
GREEN SYNTHESIS
Biosynthesis of nanoparticles is an approach of synthesizing nanoparticles using plants having biomedical applications. This approach is an environment-friendly, cost-effective, biocompatible, safe, green approach .Green synthesis includes synthesis through plants, bacteria, fungi, algae etc. NPs synthesized from biomimetic approach show more catalytic

activity and limit the use of expensive and toxic chemicals. These plant extract secrete some
phytochemicals that act as both reducing agent and capping or stabilization agent.
The "green" synthetic strategy requires the capping materials to be environmentally benign,
non-toxic and renewable. In this context, plant extract coating seems to be a promising
route to accomplish this task, based on its reliability, high protecting performance, good
stability, biocompatibility and ease of implementation.
Plants are most preferred source of NPs synthesis because they lead to large-scale
production and production of stable, varied in shape and size NPs. Bio-encapsulation
involves encapsulating vitamin with the help of phytochemicals like polysaccharides,
polyphenols compounds, amino acids, alkaloids, terpenoids secreted from the plant.
BACKGROUND OF THE INVENTION
Vitamin C, also known as ascorbic acid and L-ascorbic acid, is a vitamin found in various
foods and sold as a dietary supplement
We need vitamin Cto stay healthy: it is a vital nutrient. Without enough vitamin C, humans
can develop a disease known as scurvy.
Vitamin C was discovered in 1912, isolated in 1928, and in 1933 was the first vitamin to
be chemically produced.
Vitamin C is a water-soluble vitamin that your body needs for numerous daily functions.
It is on the World Health Organization Model List of Essential Medicines, which lists the most
effective and safe medicines needed in a health system
Vitamin C is available as an inexpensive generic and over-the-counter medication
Foods containing vitamin C include citrus fruits, kiwifruit, broccoli, Brussels sprouts, raw bell
peppers, and strawberries.[5] Prolonged storage or cooking may reduce vitamin C content in
foods

PRIOR ART
Patent application number EP1838610A1
The method/device/apparatus includes a vitamin C emitting filter, a manufacturing method thereof and a use thereof are disclosed. The vitamin C emitting filter can be manufactured by immersing the carrier in a mixed solution of the silver and the vitamin C or spraying the mixed solution of the silver and the vitamin C onto the carrier. Also, the vitamin C emitting filter can be manufactured by coating a carrier with a silver and coating a vitamin C on the carrier coated with the silver by processing a vitamin C in the carrier. Accordingly, the vitamin C emitting filter can provide the antibacterial function without influence on the property of the vitamin C and can constantly emit the vitamin C in air.
Patent application number EP1688130A1
Stable vitamin C {L-ascorbic acid) compositions for topical application, comprising micronized L-ascorbic acid in a substantially non-aqueous carrier having a pH greater than that of skin, and preferably not lower than approximately 5.5 and not greater than approximately 7.5, are described. The composition may consist essentially of L-ascorbic acid and capric/caprylic triglycerides or may further comprise papain as an enzymatic exfoliant in addition to humectants, emollients, viscosity-increasing agents, surfactants, and preservatives. Methods of preparing the micronized L-ascorbic acid for use in the described formulations, by a "wet" micronization process, are also described. The compositions are useful as UV protectants, promoters of collagen synthesis and in the removal and/or treatment of wrinkles.
Patent application number CN101558826A
The invention discloses glycine nano-selenium and a preparation method thereof. 0.9 weight portion of selenium and 85 to 95 weight portions of glycine form granular nano-selenium, the particle size of the nano-selenium is between 20 and 80 nm, and the average particle

size is 60nm. The preparation method thereof comprises the following steps that: 2 to 3 weight portions of sodium selenite is dissolved into 15 to 25 weight portions of water to obtain a sodium selenite solution at normal temperature and normal pressure, 0.5 to 1.5 weight portions of polyvinylpyrrolidone (PVP) surfactant is added into the solution to form an emulsion, then 3 to 6 weight portions of vitamin C is added into the emulsion, the vitamin C and the emulsion react-for 20 to 40 minutes at normal temperature, a reaction liquid is absorbed by 80 to 120 weight portions of glycine, and finally the obtained product is dried, crushed, sieved and packaged. The invention solves the problems that red liquid nano-selenium is not favorable for packaging and transportation, and adopts the glycine as an adsorbent and a stabilizing agent of nano-selenium to ensure that the liquid nano-selenium forms the solid glycine nano-selenium; and after the glycine nano-selenium is prepared into a nanometer additive, the nanometer additive is easy to digest and absorb, can ensure the rapid growth of beasts and birds, and can also greatly reduce the using amount, thereby reducing raising technology and improving economical benefit.
Patent application number WO2004000274A1
Present invention relates to the field of compositions of biologically active agents (nutritional supplements, drugs and pharmaceutical agents), their dosage form and new method of administration into the human body. The invention combines the idea of application of nanoemulsions as transport systems for agents and the oral delivery method of said compositions through absorption through the buccal mucosa. Said nanoemulsions contain both water-soluble and fat-soluble biologically active agents, which do not need the conventional emulsifier additives in order to show physical stability. The phase separation of the fat and water soluble particles of the solution is avoided by the addition of pure natural phospholipids. The nanoemulsions with mean droplet diameters ranging from 0,00005 to 0,0001 mm are dispersed by a mechanical pump spray into the oral cavity, where they are absorbed through the buccal mucosa and reach the blood stream and benefit the body.

Patent application number EP1263415A4
Stable vitamin C (L-ascorbic acid) compositions for topical application, comprising micronized L-ascorbic acid in a substantially non-aqueous carrier having a pH greater than that of skin, and preferably not lower than approximately 5.5 and not greater than approximately 7.5, are described. The composition may consist essentially of L-ascorbic acid and capric/caprylic triglycerides or may further comprise papain as an enzymatic exfoliant in addition to humectants, emollients, viscosity-increasing agents, surfactants, and preservatives. Methods of preparing the micronized L-ascorbic acid for use in the described formulations, by a 'wet' micronization process, are also described. The compositions are useful as UV protectants, promoters of collagen synthesis and in the removal and/or treatment of wrinkles.
Patent application number EP1263415A4
Stable vitamin C (L-ascorbic acid) compositions for topical application, comprising micronized L-ascorbic acid in a substantially non-aqueous carrier having a pH greater than that of skin, and preferably not lower than approximately 5.5 and not greater than approximately 7.5, are described. The composition may consist essentially of L-ascorbic acid and capric/caprylic triglycerides or may further comprise papain as an enzymatic exfoliant in addition to humectants, emollients, viscosity-increasing agents, surfactants, and preservatives. Methods of preparing the micronized L-ascorbic acid for use in the described formulations, by a 'wet' micronization process, are also described. The compositions are useful as UV protectants, promoters of collagen synthesis and in the removal and/or treatment of wrinkles.
Patent application number . US7101563B1
Stable vitamin C (L-ascorbic acid) compositions for topical application, comprising micronized L-ascorbic acid in a substantially non-aqueous carrier having a pH greater than that of skin, and preferably not lower than approximately 5.5 and not greater than approximately 7.5, are described. The composition may further comprise papain as an enzymatic exfoliant in

addition to humectants, emollients, viscosity-increasing agents, surfactants, and preservatives. Methods of preparing the micronized L-ascorbic acid for use in the described formulations, by a "wet" micronization process, are also described. The compositions are useful as UV protectants, promoters of collagen synthesis and in the removal and/or treatment of wrinkles
Patent application number 201821042806
Abstract In This Invention the waste steam convert into a more than three usefully thing Tower Generation, Filter Water and Protein, Vitamin, through Pressure Cooker Steam." In this technology which produces three main by products that is "Electricity, Distilled Water" and Protein, Vitamin by using pressure cooker steam. This invention is very cost effective because doesn"t require any large amount of fuel, coal, wood to produce electricity. This technology does not use reserve earth material for production of electricity. My invention . focus on the electricity generation from the pressure cooker steam which is used for normal cooking purpose inside the kitchen initially cooker used to cook food in less time and at low cost. But that cooker only introduced to generate electricity. Here with the help of pressure cooker steam turbine is moved and mechanical energy sends to generator to create electricity. Once pressure cooker vessels steam goes out, that time that steam is provided to the turbine to get start and generate electricity. After electricity generation again that waste steam is used to transform into distilled water and abstraction Protein, Vitamin. This can be possible with the help of condenser and air cooling system. Steam came out is cooled in the condenser and air cooling system. That transformed water is collected in the pipe and went through activated carbon filter to remove impurities. Thus the filtered water is collected in the water storage and used for household purpose. And available impurities apply the abstraction technique of Protein, Vitamin, to abstract the available Protein, Vitamin in these impurities this invention produces three products which are more demanding nowadays. Water is the natural source depends on the rain cannot be created by any ways. But by using such a methodology water can be reused multiple times from water to steam and again from steam to water. Such a technology can be placed in the house, hotels, restaurants where
large amount of steam goes out during cooking time, this is the small scale project but this
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technology can be applied on the natural sources of water where steam goes out due to sun or any other reason. This invention will solve the world"s biggest problem in low cost.
Patent application number 201741037449
ABSTRACT ESTIMATION OF VITAMIN C LEVELS BY IMPROVEMENT IN THE METHODOLOGY Disease of vitamin C deficiency that might reflect its role as an antioxidant include an increased risk of coronary heart disease, and an increased risk of death by strike in elderly people. Some scorbutic patients may develop anaemia, display radiological changes characteristic of osteoporosis or die suddenly from heart failure. Aim of this invention is to estimate Vitamin C levels accurately by best method.
Patent application number 201627037881
The invention relates to a composition comprising A) microcapsules comprising at least one fat soluble active substance selected from a vitamin K compound or a provitamin or a prodrug of a vitamin K compound embedded in a matrix comprising a hydrocolloid and optionally one or more other matrix components and B) at least one dietary mineral; as well as uses and products comprising such compositions.
Patent application number 201741013728
A process for the preparation of a nano foliar spray fertilizer containing one or more micronutrients in nano size, a plant protector, an organic natural chelator and a wetting agent that fortify the crop with iron, zinc, carotenoids and vitamin Acomprises the following steps: preparation of an essential micronutrients such as zinc nano panicles, iron nano particles, plant growth booster such as an aqueous extract of Spintlina platensis (5%). plant protector and a water absorbing hydrogel prepared in a natural organic acid such as a,chitosan prepared ( \% ) in ■ aqueous extract of Tamarinclus indica(2OgAO0m\), a wetting agent such as glycerol, a biocontrol agent such as 20 ml of neem leaf extract and 5 ml of neem oil. The finished fertilizer contains 1% by weight of zinc and I % by weight of iron.

Patent application Nanocapsulated Ascorbic Acid in Combating Cerebral Ischemia Reperfusion- Induced Oxidative Injury in Rat Brain
Recent evidences suggest that cerebral ischemia-reperfusion insult plays significant role in pathogenic diseases like Alzheimer's disease (AD) and other neurodegenerative diseases. Toxic reactive oxygen species (ROS) generated by induced oxidative stress in the episodes of cerebral ischemia-reperfusion (CIR) plays major role in neurodegeneration. As the prime source of ROS generation, neuronal mitochondria, the cellular energy metabolic centre experience severe damage because of CIR-induced oxidative stress. The process of mitochondrial dysfunction is accelerated by CIR that may pave the pathway for neurodegeneration in AD among aged individuals. Prevention of CIR injury may be a shunt in order to minimize the risk of dementia of Alzheimer's type in aged individuals. The use of chemical antioxidants in CIR is not suitable as the blood- brain barrier (BBB) doesn't allow the entry of molecules from blood circulation into the brain. Thus L-ascorbic acid loaded polylactide nanocapsules were prepared and fed orally to assess the role of nanocapsulated ascorbic acid (IMAA) against CIR induced oxidative injury in mitochondrial region of rat brains. Mitochondrial injury was assessed by the extent of lipid peroxidation and in situ antioxidant enzyme status. The levels of cytochrome c (cyt c), cyclooxygenase- 2 (COX-2) and iNOS were determined. Results showed that in comparison to free ascorbic acid (AA), NAA exerted better protection to the brain mitochondria by preventing oxidative damage in ROS mediated CIR injury.
Influence of Nano-ZnO Exposure to Plants on L-Ascorbic Acid Levels: Indication of Nanoparticle-lnduced Oxidative Stress.
With the continuously growing production of nanomaterials, their presence in the environment increases. The effects of nanoparticle exposure on plants are yet not fully understood. This study aimed to provide new insights into the stress induced by the exposure of plants to nanoparticles via the determination of the content of L-ascorbic acid, known antioxidant, in the samples of bryophyte species Pleurozium schreberi (Brid.) Mitt.

Bryophyte samples, collected from a pristine environment, were exposed to 0.1 g-L-1 suspensions of nano-ZnO for five weeks. Prior to the exposure, the samples were subjected to the combination of treatments-washing or not washing as well as drying or irrigation. In order to assess the level of oxidative stress caused by the exposure to the ZnO nanoparticles under various pre-treatment conditions, the content of the L-ascorbic acid in the samples was determined by titration with dichlorophenolindophenol. Effect of both nano-ZnO exposure and pre-treatment was observed; nano-ZnO exposure significantly reduced the content of L-ascorbic acid in the bryophyte samples indicating it being consumed in the repair processes following the exposure-related physiological stress.
LITERATURE CITED
♦ Padayatty S.J., Katz A. and Wang Y. (2003). Vitamin C as an antioxidant: evaluation of its role in disease prevention. Journal of American College of Nutrition, 22:18-35.
♦ Pardue S.L. and Williams S.H. (1990). Ascorbic acid dynamics in avian neonates during stress.ln: Ascorbic acid in domestic animals.Proc. 2nd SymposiumKartauselttingen, Switzerland.
♦ Lin H., Jiao H.C., Buyse J. and Decuypere E. (2006). Strategies for preventing beat stress in poultry. World's Poultry Science Journal, 62(l):71-86.
♦ Kutlu H.R. and Forbes J.M. (1993). Changes In growth and blood parameters in heat-stressed broiler chicks in response to dietary ascorbic acid. Livestock Product Science, 36:335-350.
♦ Hodges R.E., Hood J., Canham J.E., Sauberlich H.E. and Baker EM. (1971). Clinical manifestations of ascorbic acid deficiency in man.American Journal of Clinical Nutrition, 24:432- 443.
♦ El-Boushy A.R. and Van Albada M. (1970). The effect of vitamin C on egg shell quality under high environment al temperatures. Netherland Journal of Agricultural Science, 18:62

SUMMARY OF INVENTION
A nanoparticle is a piece of material that is so small it has to be measured in nanometers. One nanometer equals one-billionth of a meter. For comparison, 10,000 nanoparticles could fit in the diameter of a human hair .Nano particles refer to a particle size of roughly 1 to 100 nm .At this scale the physical, chemical and biological properties of material differ fundamentally and often unexpectedly. These nanomineral particles are having higher potential than their conventional sources and thus reduce the quantity required. The water-soluble vitamin C is probably the most well-known vitamin. Even before its discovery in 1932, physicians recognized that there must be a compound in citrus fruits preventing scurvy, a disease that killed as many as two million sailors between 1500 and 1800. Later researchers discovered that man, other primates and the guinea pig depend on external sources to cover their vitamin C requirements. Most other animals are able to synthesize vitamin C from glucose and galactose in their bodies.
DRAWING:
Fig 1, Illustrating shows the flow chart of NANO ENCAPSULATEDVITAMIN C in which Incubation period results in a change of color of the mixture to Pink which is a visual confirmation of the synthesized NPs . Further, synthesized nanoparticles are further characterized using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM)
Fig 2, shows the SEM technique was employed to visualize the size of nano encapsulated vitamin C. The formation of nano encapsulated vitamin C as well as their morphological dimensions in the SEM analysis demonstrated that the average size was from 82-124 nm with inter-particle distance.
Fig 3, is Sample of Nano encapsulated Vitamin C
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Fig 4, Shows the The dual role of the plant extract as a reducing and capping agent and presence of some functional groups was confirmed by FTIR analysis . A broad band between 3441.12 cm-1 is due to the N-H stretching vibration of group NH2 and OH the overlapping of the stretching vibration of attributed for DMSO and plant extract molecules. The observed peaks at 1028 cm-1 denote -C-OC- linkages, or -C-O- bonds. The observed peaks are mainly attributed to flavanoids and terpenoidsexcessively present in plants extract. From FT-IR results, it can be concluded that some of the bioorganics compounds from plant extract formed a strong coating/capping on the nanoparticles.
DETAILED DESCRIPTION OF THE INVENTION
Bio-availability of Vitamin C
Vitamin C, a well-known antioxidant, is an essential nutrient involved in a variety of dermatological, pharmaceutical and biological functions, such as reducing melanin, eliminating free radicals, promoting collagen biosynthesis and improving the organism immunity. Therefore, vitamin C has become the focus in various fields, particularly in the field of livestock and poultry industry. However, vitamin C is unstable and can be easily oxidized in the presence of oxygen, humidity, high temperatures and heavy metals during the storage and processing. This could cause an obvious decrease of its utilization. Furthermore, another major disadvantage of vitamin C is its low penetration rate through the skin, due to its aqueous solubility making it difficult to penetrate through the diffusional barrier of the stratum corneum or the horny layer. In order to overcome thesedisadvantages, extensive studies have been tried, such as derivatizing vitamin C into a salt, encapsulating of vitamin C by using liquid crystals,microemulsions, and inorganic nano encapsulation. However, the instability problem of vitamin C remains a challenge. Recent studies suggest that the bioavailability of nanoencapsulated components is higher than conventional sources because of the small particle size and high surface to volume ratio. It is a great challenge to prepare nano encapsulated vitamin with high entrapment and good size distribution.

PREPARATION OF PLANT EXTRACT
The plant part is washed thoroughly in running tap water and sterilized using double distilled water Then, the plant part is kept for drying at room temperature followed by weighing and then crushing it using a mortar and pestle.
DMSO (Dimethyl Sulfoxide) is added to the plant part according to the desired concentration and continuous stirring using a magnetic stirrer. The solution is filtered using Whatman filter paper and the obtained clear solution was used as a plant extract.
Advantages of Nano Vitamin C
♦ Vitamin C plays an important role in the biosynthesis of corticosterone which is a hormone that enhances energy supply during stress.
♦ Nano vitamin C is having a great potential as antioxidant in broiler chicken production even at very lower doses than the conventional sources.
♦ Effective delivery of functional compounds to target sites and thereby better bioavailability.
♦ Vitamin C supplementation in poultry feeds has been reported to have positive effects such as weight gain and improved immune response.
♦ It is also a vital component in wound healing.

CLAIMS We CLAIM
1. A method of synthesizing Nano encapsulated Vitamin C , comprising the steps of providing a solution of vitamin C& providing plant extract solution and combining the vitamin C solution and the plant extract solution while stirring at room temperature to produce the nanoencapsulated Vitamin C .
2. The method of synthesizing nano encapsulated Vitamin C according to claim 1, where L-ascorbic acid used as a Vitamin C source.
3. The method of synthesizing nano encapsulated Vitamin C according to claim 1, where DMSO used as a solvent.
4. The method of synthesizing nano encapsulated Vitamin C according to claim 1, where unknown plant extract used as a encapsulating agent.
5. The method of synthesizing nano encapsulated Vitamin C according to claim 1, wherein said nano encapsulated Vitamin C have a mean diameter in the range from about 82 to about 124 nm.