Claims:We claim:
1. A natural extract formulation comprising extracts of Onion (Allium cepa), Garlic (Allium sativum), Lilac tassel flower (Emilia sonchifolia), Malabar nut (Adathoda vasica), Indian borage (Plectranthus amboinicus), Turmeric (Curcuma longa), Nutmeg (Myristica fragrans) & Black pepper (Piper nigrum).

2. The natural extract formulation of claim 1, comprises Onion (Allium cepa): 22-28%, Garlic (Allium sativum): 22-28%, Lilac tassel flower (Emilia sonchifolia): 12-18%, Malabar nut (Adathoda vasica): 12-18%, Indian borage (Plectranthus amboinicus): 12-18%, Turmeric (Curcuma longa): 1-3%, Nutmeg (Myristica fragrans): 0.1-0.5% and Black pepper (Piper nigrum): 0.05-0.15%.

3. The natural extract formulation of claim 1 and 2, includes organic and aqueous solvent extracts.

4. The natural extract formulation of claim 1, further comprises a water soluble additive.

5. The natural extract formulation of claim 1 and 4, wherein the water soluble additive is Polysorbate 80.

6. The natural extract formulation of claim 1, 4 and 5, wherein the water soluble additive is in a concentration ranging from 1-2%.

7. The natural extract formulation of any of the preceeding claims, is a water soluble formulation.

8. The natural extract formulation is administered to poultry in a concentration from about 1ppm to about 15 ppm.

9. The natural extract formulation of claim 8, is administered to poultry in a preferable concentration from about 5ppm to about 10ppm.

10. The natural extract formulation of any of the preceeding claims is formulated in the form of solution, powder, granules, water dispersible tablet.

11. The natural extract formulation of any of the preceeding claims is provided to the poultry by dissolving in drinking water.

12. A method of improving overall poultry health, immunity, laying bird egg count and decreasing bird mortality using the natural extract formulation comprising extracts of Onion (Allium cepa), Garlic (Allium sativum), Lilac tassel flower (Emilia sonchifolia), Malabar nut (Adathoda vasica), Indian borage (Plectranthus amboinicus), Turmeric (Curcuma longa), Nutmeg (Myristica fragrans) and Black pepper (Piper nigrum).

13. The natural extract formulation of any of the preceeding claims prevents poultry diseases such as chronic respiratory disease, osteoarthritis and jaundice, helps in promoting poultry weight gain, improves the quality and flavor of meat and is free of any antibiotic residue.
Dated this 1st day of July, 2018
FOR ARISHINA LIFE SCIENCES PVT. LTD.


(APEKSHA SAWANT)
IN/PA-1995
, Description:
FORM – 2
THE PATENTS ACT, 1970
(39 of 1970)
The Patents Rules, 2006

COMPLETE SPECIFICATION
(See Section 10 and Rule 13)

NATURAL EXTRACTS FORMULATION FOR IMPROVING POULTRY HEALTH

Arishina Life Sciences Pvt Ltd, 9/304, Chiramel House, Near St. Joseph’s Convent Mission Quarters, Trichur, Kerala – 680001, India

The following specification particularly describes the invention and the manner in which it is to be performed.
Field of the invention

The present invention relates to a water soluble formulation derived from natural sources for improving the overall poultry health and number of eggs in laying birds. More particularly, the invention relates to a water soluble formulation derived from herbs and spices for poultry health and increasing eggs count in laying birds.

Background of the invention

Poultry is one of the fastest growing segments of the agricultural sector. From a backyard activity it has got transformed into well-organized, scientifically oriented and technologically driven industry. In the coming years due to urbanization and rising income the affordability of chicken meat and eggs has increased leading to a greater demand for poultry products. The egg production has gone up by billions from few millions and the broiler production has gone to million tons from nowhere. Tremendous technical work has gone into broiler production in the fields of genetics, nutrition, breeder management, hatchery management, housing and disease management. The broiler growing period has gradually come down to less than 40 days from 60 days.

Success of poultry production rests primarily on the quality of the bird employed, comforting environment and provision for good feed, the last being most expensive of all other inputs, deserves befitting attention. Considerable research centers on improving the efficiency of feed utilization. In addition to cost associated with feed, disease control constitutes a high priority in raising poultry. Due to the large number of poultry confined in small area, emphasis is directed to preventing disease at the onset rather than curing the disease.

The production of antibiotic-free (ABF) poultry is now a common trend worldwide, since growth promotant antibiotics (GPA) have been banned by some governments and because many consumers are increasingly avoiding food products that contain antibiotics. There are many categories in ABF production, depending on the type of certification, market claimsor products to be offered to consumers. Some of these main groups are: Standard ABF programs (use of chemical antibacterials, chemical anticoccidials and ionophores, but no antibiotics); Organic production (no antibiotics, ionophores coccidiostats, chemical anticoccidials or chemical antibacterials); Program that does not allow chemical antibacterials (but allow chemical or ionophore coccidiostats). Each system may raise certain difficulties for producers managing flock health like they could implicate higher costs of production, lower levels of productivity and difficulties in achieving low mortality and good poultry welfare.

As breeders can transfer intestinal microbes and immunity to their progeny, companies practicing ABF programs should make sure that intestinal health is adequate in breeders and that their vaccination programs are effective, to prevent issues in the progeny.

Some diseases that affect poultry are infectious, parasitic, respiratory, metabolic and nutritional. Chronic respiratory disease (hereinafter referred as CRD) has been reported in poultry. CRD is a specific disease caused by organism Mycoplasma gallisepticum. Infection with Mycoplasma gallisepticum is associated with slow onset, chronic respiratory disease in poultry. The spreading of infection is extensive due to exposure to wild birds and stress conditions like handling, overcrowding, poor litter management etc. Parent birds infected with Mycoplasma gallisepticum can transmit the organisms through eggs to their offspring (vertical transmission). In addition, infection can occur by contact or by airborne dust or droplets (horizontal transmission).The birds affected by respiratory distress show lack of appetite, decreased weight gain and poor feed conversion ratios. Drop in egg production between 20-30% can occur in laying birds. Birds that are infected with Mycoplasma gallisepticum remain carriers of the disease throughout their lives. Various antibiotics have been prescribed fortreating CRD in poultry. Commonly used antibiotics are Enterofloxacine, Levofloxacine, Oxytetracyclins, Neomycine, Ciprofloxacine etc. Some antibiotics such as Tylosin or tetracyclines, fluoroquinolones (available by prescription through a licensed veterinarian) can reduce clinical symptoms but will not completely eliminate Mycoplasma gallisepticum. However, the therapeutic benefits of the aforementioned antibiotics are often accompanied by unwanted side effects. More importantly, some antibiotics cannot be used for birds raised for meat and eggs. Even if birds have been treated with antibiotics, they can still spread Mycoplasma gallisepticum to other birds. Treatment of birds using antibiotics can be very expensive.

The most common non-infectious disease in poultry is Osteoarthritis. This can be a primary condition in birds or be secondary to problems or injury of the joints or supporting tissues of the bird. It is an age related degeneration of joint tissue that occurs in older birds due to longer duration spent by bird on their feet. Abnormal cartilage mass in the proximal head of the tibiotarsus bone is the main cause of osteochondrosis. In this condition the tibial cartilage does not mature enough to ossify (turn into bone). This leaves the growth plate prone to fracture, infection and deformed bone development. It has been seen in all fast growing types of meat birds but is most common in broiler chickens. Deficiency of nutrients like calcium, phosphorus, manganese and zinc in feed may cause this condition. Providing an appropriate feed will help in managing osteoarthritis and minimizing systemic inflammation in poultry birds.

Another disease that affects the poultry birds is jaundice which is most commonly seen in the mucosal tissue in the eyes and eye lids as well as in the tongue and inner part of mouth. Fatty liver hemorrhagic syndrome is a common condition in broilers. Hot humid climate with high level of protein in the feed may cause this condition.

The treatment of the aforesaid diseases in poultry involves use of antibiotics, synthetic chemicals, herbal medicine and electrolytes. Some of the publications are –
US3344022A relates to use of a series of quinoxaline-di-N-oxides as agents for the control of infectious sinusitis in turkeys, chronic respiratory and complicated chronic respiratory infections in poultry and for the promotion of weight gain and feed consumption of animals.

US6682762B2 provides a poultry and livestock feed additive composition containing electrolytes, roughage and mineral oil to increase dietary electrolyte balance.

CN101406246A provides herbal medicine feed additive for chickens comprising Radix, astragalus, Houttuynia, Codonopsis, wolfberry, scutellaria, light bamboo, orange peel, angelica, eucommia, ash bark, Pulsatilla, Baimaoteng, white peony root, Rehmannia, tea leaves and pine needles.

CN101647521A discloses an egg promoting feed additive for poultry mainly prepared from codonopsis pilosula leaves, astragalus root, epimedium, glossy privet fruit, folium artemisiae argyi, notherwort herb, acanthopanax root, hawthorn, humifuse euphorbaia herb, scurfpea fruit, pine leaves and radix polygoni multiflori preparata.

Currently used antibiotics have many limitations. Apart from being prescription drugs requiring a continuous medical supervision during treatment, they have serious side effects. Treatment of birds using antibiotics can be very expensive. Moreover, antibiotics cannot be used as a part of treatment in birds raised for meat and eggs. The disadvantage of using known formulations in poultry is that they have limited bioavailability and thereby limited replaceability of antibiotics. These drawbacks and rationales have lead the inventors of the present invention to develop a composition that is efficacious, economical and effective for improving poultry health and which increases the meat quality and egg count.

Object of the invention

An object of the present invention relates to a natural extract formulation that improves the overall health of poultry, improves the immunity, decreases the mortality and increases the egg count in the laying birds.

Another object of the present invention is to prevent poultry diseases such as chronic respiratory disease, osteoarthritis and jaundice.

Yet another object of the invention is to provide a natural formulation that helps in promoting poultry weight gain by enhancing the feed consumption efficiency.

Still another object of the present invention is to provide a formulation that improves the quality and flavor of meat that is free of any antibiotic residues.

Summary of the invention

In an embodiment, the present invention relates to a water soluble formulation of natural origin. More particularly the present invention relates to a water soluble natural extract comprising of Onion, Garlic, Lilac tassel flower, Malabar nut, Indian borage, Turmeric, Nutmeg and Black pepper.

The present invention relates to a water soluble composition of natural extracts comprising Onion 22-28%, Garlic 22-28%, Lilac tassel flower 12-18%, Malabar nut 12-18%, Indian borage 12-18%, Turmeric 1-3%, Nutmeg 0.1-0.5% and Black pepper 0.05-0.15%.

In another embodiment, the water soluble natural extract composition is administered to poultry like chicken, turkey, game birds, pigeons, duck, geese and wild birds in a concentration from about 1ppm to about 13ppm. More particularly the concentration of the natural extract composition ranges from about 5ppm to 10ppm.

Yet another embodiment of the present invention, relates to a water soluble composition for improving overall poultry health; prevent chronic respiratory disease, arthritis and jaundice; promote weight gain; increase feed consumption efficiency; improve immunity; improve quality and flavor of meat; lower mortality rate and increase egg production.

In still another embodiment, the present invention relates to a process for preparing the water soluble natural extract composition comprising blending together the extracts of Onion, Garlic, Lilac tassel flower, Malabar nut, Indian borage, Turmeric, Nutmeg and Black pepper as per the ratio. The contents are mixed to get a uniform liquid blend. The blended composition can be formulated in the form of powders, granules, water dispersible tablets and the like by the processes know to a person skilled in the art and should be administered by dissolving in drinking water.

Detailed description of the invention

In the past five decades the demand for poultry meat has risen steeply at around three times to the rate of population growth, according to the United Nations' Food and Agriculture Organization (FAO). In order to supply this increasing demand both more efficient production of meat and greater effectiveness of resource utilization in poultry operations is necessary. Tremendous scientific works has gone into broiler production in the field of nutrition, breeder management, genetics, hatchery management, housing and disease management.

To increase the revenue of poultry enterprise, it is utmost important to know how to improve the Feed Conversion Ratio and how to reduce the feed costs. The feed conversion ratio (FCR) is the amount of feed ingested by an animal which can be converted into one kilo of live weight. Poultry industries have used feed efficiency (feed to gain or gain to feed ratio) as a benchmark for profitability. For example, in the poultry industry, even a slight increase in broiler growth rate coupled with improved feed efficiency brings the broiler chicken to market maturity faster at a lower cost.

The composition of the present invention relates to a method of increasing the feed rate of poultry and decreasing the length of time required to reach market weight. This leads to a more efficient use of the animal facilities and reduces the resources needed to produce meat.

In an embodiment, the present invention relates to a water soluble formulation of natural origin. The advantage of the composition of the present invention is that it replaces antibiotics with supplements derived from natural sources. Further the water soluble composition enhances the bioavailability of the active ingredients.

In another embodiment the water soluble composition of the present invention comprises the extracts of Onion (Allium cepa), Garlic (Allium sativum), Lilac tassel flower (Emilia sonchifolia), Malabar nut (Adathoda vasica), Indian borage (Plectranthus amboinicus), Turmeric (Curcuma longa), Nutmeg (Myristica fragrans) and Black pepper (Piper nigrum). The percentage of each ingredient in the composition is as follows:
Onion Extract: 22-28%
Garlic Extract: 22-28%
Lilac tassel flower Extract: 12-18%
Malabar nut Extract: 12-18%
Indian borage Extract: 12-18%
Turmeric Extract: 1-3%
Nutmeg Extract: 0.1-0.5%
Black Pepper Extract: 0.05-0.15%.

The composition further comprises water soluble additives like Polysorbate 80 in a concentration of 1-2%.

The onion extract is obtained using fresh or dehydrated bulbs. The flaked bulbs are added in an extractor along with water and boiled at 80-100°C. The extract obtained is cooled, filtered and concentrated under vacuum at less than 80°C to obtain water soluble onion extract.

The garlic extract is obtained using fresh or dehydrated bulbs. The flaked bulbs are added in an extractor along with water and boiled at 80-100°C. The extract obtained is cooled, filtered and concentrated under vacuum at less than 80°C to obtain water soluble garlic extract.

The lilac tassel flower extract is obtained from fresh or dried herb. The flaked /powdered herbs are added in an extractor along with water or a mixture of ethanol and water (50:50) and boiled at 80-100°C. The extract obtained is cooled, filtered and concentrated under vacuum at less than 80°C to obtain water soluble lilac tassel flower extract.

The Malabar nut extract is obtained from fresh or dried herb. The flaked / powdered herbs are added in an extractor along with water or a mixture of ethanol and water (50:50) and boiled at 80-100°C. The extract obtained is cooled, filtered and concentrated under vacuum at less than 80°C to obtain water soluble Malabar nut extract.

The Indian borage extract is obtained from fresh or dried herb. The herbs are added in an extractor along with water or a mixture of ethanol and water (50:50) and boiled at 80-100°C. The extract obtained is cooled, filtered and concentrated under vacuum at less than 80°C to obtain water soluble Indian borage extract.

The turmeric extract was obtained by grinding turmeric rhizome and sieving. 1 part of the obtained powder was extracted using 3 parts of ethanol/ water mixture (50:50). The contents were concentrated to obtain turmeric oleoresin (curcumin content- 30%). The extraction can also be carried out using solvents like acetone, hexane and ethyl acetate.

The nutmeg extract was obtained by grinding dried fruit. 1 part of the obtained powder was extracted using 3 parts of ethanol/ water mixture (50:50). The contents were concentrated to obtain nutmeg oleoresin (myristicin content- 10%). The extraction can also be carried out using solvents like acetone, hexane and ethyl acetate.

The black pepper extract was obtained by flaking /grinding dried black pepper. 1 part of the obtained powder was extracted using 3-4 parts of ethanol/ water mixture (50:50). The contents were concentrated to obtain black pepper oleoresin (Piperine content- 40%). The extraction can also be carried out using solvents like acetone, hexane and ethyl acetate.

In an embodiment, the present invention relates to a process for preparing the water soluble natural extract composition comprising blending together the extracts of Onion, Garlic, Lilac tassel flower, Malabar nut, Indian borage, Turmeric, Nutmeg and Black pepper as per the ratio mentioned above. The contents are mixed to get a uniform liquid blend.

The blended composition is dispensed as liquid or spray dried by processes known to a person skilled in the art. The spray dried product is then formulated as powder, granules, water dispersible tablets and the like by the processes know in the art.

In yet another embodiment the water soluble natural extract composition is administered to poultry like chicken, turkey, game birds, pigeons, duck, geese and wild birds in a concentration from about 1ppm to about 15ppm. More particularly the concentration of the natural extract composition ranges from about 5ppm to 10ppm.

In further embodiment the composition containing natural extracts is administered to the poultry beginning from 1 day of age till the entire life.

In still another embodiment the water soluble composition is used for improving overall poultry health; prevent chronic respiratory disease, arthritis and jaundice; promote weight gain; increase feed consumption efficiency; improve immunity; improve quality and flavor of meat; lower mortality rate and increase egg production.

In an embodiment, the invention is a water soluble formulation for poultry for weight gain comprising feeding the said formulation containing natural extracts to the poultry through drinking water wherein the concentration of formulation containing natural extract is from about 5ppm to about 10ppm and is administered every day.

In another embodiment, the composition of the present invention improves the overall health of poultry birds and prevents the chronic respiratory disease by feeding the said formulation containing natural extracts to the poultry through drinking water wherein the concentration of the formulation is from about 5ppm to about 10ppm and is administered every day.

In an embodiment, the invention is used for improving overall health and preventing arthritic problem in poultry by feeding the formulation through drinking water every day in the concentration from about 5ppm to about 10ppm.

In an embodiment, the invention is used for improving the feed efficiency (FE) of poultry by feeding the formulation through drinking water every day in the concentration from about 5ppm to about 10ppm.
In an embodiment, the invention is used for improving the immunity of poultry by feeding the formulation through drinking water every day in the concentration from about 5ppm to about 10ppm.

In an embodiment, the invention is used for decreasing the mortality rate to below 0.5% of poultry by feeding the formulation through drinking water every day in the concentration from about 5ppm to about 10ppm.

In an embodiment, the invention is a water soluble formulation used for improving the egg production of poultry comprising feeding the said formulation containing natural extracts to the poultry through drinking water wherein the concentration of formulation containing natural extract is from about 5ppm to about 10ppm and is administered every day.

In another embodiment, the present invention improves the quality and flavor of the meat by feeding the poultry with a formulation dissolved in drinking water in a concentration of about 5ppm to about 10ppm.

The advantage of the water soluble composition is that it eliminates the need for antibiotic administration and hence resulting in zero antibiotic residue in the egg and meat as measured using the Charm’s multipurpose Liquid Scintillation Counter (LSC) and Luminometer System and Charm’s Rapid One Step Assay (ROSA) Reader. The antibiotic content for all the trials done was either zero or not detected.

The following examples are for the purpose of illustration of the invention only and are not intended in any way to limit the scope of the present invention.

Examples of the invention

Example 1:
Step A) Preparation of water soluble formulation containing natural extracts -
Dried Turmeric rhizomes (100g) were flaked, powdered and extracted with ethanol/water mixture (50:50) in a separating funnel using cold percolation process which was carried out for a period of 3hr. The extraction procedure was repeated and the extracts were pooled before concentrating. The resulting extract was filtered and concentrated by distillation under vacuum at temperature less than 80°C to provide turmeric oleoresin (Yield- 16g; Curcumin- 30%).
Dried Black Pepper (100g) was flaked, powdered and extracted with ethanol/water mixture (50:50) in a separating funnel using cold percolation process performed for 3hr. The extraction procedure was repeated and the extracts were pooled before concentrating. The resulting extract was filtered and concentrated by distillation under vacuum at temperature less than 80°C to produce Black Pepper oleoresin (Yield- 5g; Piperine- 40%).
In a separating funnel, 100g of ground dried Nutmeg was added. It was extracted using ethanol/water mixture (50:50) by performing cold percolation process for 3hr. This process was repeated and the extracts obtained were pooled and concentrated. The resulting concentrate was filtered and distilled under vacuum at less than 80°C to produce nutmeg oleoresin (Yield- 15g; myristicin- 10%).
Raw Lilac tassel flower (100g) was flaked and boiled with 400 ml water in a round bottom flask for 1 hr at 80°C. The material was cooled, filtered and concentrated by distillation under vacuum at temperature less than 80°C to produce Lilac water extract (Yield- 100g).
Dried Malabar nut (100g) was flaked and boiled with 400 ml water in a round bottom flask for 1hr at 80-90°C. The extract obtained was cooled, filtered and concentrated by distillation under vacuum at temperature less than 80°C (Yield- 100g).
In a round bottom flask, raw onion (100g) was flaked and boiled with water (400 ml) for 1hr at 80-100°C. The extract was cooled, filtered and concentrated by distillation under vacuum at temperature less than 80°C (Yield- 100g).
100g of raw garlic was flaked and boiled with 400 ml water in a round bottom flask for 1hr at 80-100°C. The material was cooled, filtered and concentrated by distillation under vacuum below 80°C to produce garlic water extract (Yield- 100g).

Step B) Formulation preparation -
In a flask, the following extracts in the mentioned ratio were added -
Lilac tassel flower extract : 12-18 %
Malabar Nut extract : 12-18 %
Indian Borage extract : 12-18 %
Onion Extract : 22-28 %
Garlic Extract : 22-28 %
Nutmeg Extract : 0.1-0.5 %
Pepper Extract : 0.05-.15 %
Turmeric Extract : 1-3 %
All the contents were heated to 90-100°C until homogeneous solution was obtained. The solution was cooled and Polysorbate 80 (1-2%) was added. The temperature was raised to 85-90°C, contents were mixed and solution obtained was cooled to room temperature.

Example 2:
Trial on broiler birds to understand the efficacy of the formulation containing natural extracts -
A trial was carried out on Komerla broiler birds to understand the efficacy of the formulation containing natural extracts. A total of 1,515 of 1 day old chicks were obtained from a commercial hatchery and allocated at random to the experimental batches. The control batch contained 815 chicks and the treatment batch contained 700 chicks. The birds were housed in pens with a stocking density of 1.2-1.31 sq feet/bird. The chicks were observed till 40 days of age. Birds were subjected to 24 hours light throughout the trial and had access to feed and drinking water.
The chicks were fed with feed (SKM brand Starter and Finisher feeds comprising yellow corn, soybean meal, vitamin premix and trace mineral mix with no added growth promoter or veterinary antibiotics). Starter feeds were fed from 0 to 24 days of age and finisher feeds from 25 to 40 days of age. Vaccines were administered through drinking water on 5th day of age Newcastle disease vaccine (LaSota strain); on 12th day of age Infectious Bursal disease vaccine (live virus) and on 21st day of age Newcastle disease vaccine (LaSota strain).

All regular antibiotics and liver tonics were given to the control batch. Treatment batch was exempted from antibiotics and liver tonics.

Normal drinking water at neutral pH was fed to the control batch whereas the formulation of the present invention containing natural extracts (5ppm) was dissolved in drinking water and fed to the treatment batch. Quantity of water consumed by both batches was measured on daily basis.

Observations included growth, body weight, feed intake, feed efficiency, general health, drinking water intake, mortality rate, meat quality and antibiotics content in meat and liver.

No adverse events were noted in the treatment batch during the study. Significant difference was observed in body weight of birds between treatment and control batch.
There was 1 death observed between 0 and 10 days, 11 and 20 days and 21 and 30 days in treatment batch. No death was observed between 31 and 40 days in treatment batch. 5 deaths were observed between 1-10 days, 11 deaths between 11-20 days, 10 deaths between 21-30 days and 5 deaths between 31-40 days in control batch.

The mortality percentage was 0.43% in treatment batch and 3.84% in control batch. Water consumption was 6.54 liter/ bird and 6.40 liter/ bird in treatment and control batch respectively. FCR was 1.77 and 1.78 kg respectively.
Mortality
Very low mortality occurred during the study in treatment batch. A total of 3 mortalities (0.43%) occurred during the treatment period (0-40 days) whereas total of 31 mortalities were observed in control batch (3.84%) during the period (0-40 days).
Body Weight
The final average body weight of the control batch was 2.19 kg/bird within 40 days. There was significant increase in final body weight of treatment batch within 40 days. The average body weight/bird was 2.28 kg. The large body weight increase (86.10gm/bird) in treatment batch noted above with control batch with broiler chicken indicates the magnitude of this study, effect and advantage of the formulation. The formulation improved body resistance, livability and overall performance of the birds. It maintain normal metabolic functioning of the bird and positive effect on weight gain.

The trial and its observations are summarized in table below:

Trial No: ZM/D/2017-18
GENERAL INFORMATION
Observations Treatment batch Control batch
Number of Birds 700 815
Date started 9th Sept 2017 9th Sept 2017
Date finished 19th Oct 2017 19th Oct 2017
Total number of days 40 40
Antibiotic given None All regular
Other medicine None Liver tonic given for all days
Average WBT(°C) 28.55 28.55
Area Utilisation (Sq feet/ bird) 1.31 1.29
Average pH of drinking water 7.03 7.03
Quantity of water consumed (Ltr/Bird) 6.54 6.40
Dosage of Formulation applied (ml/Ltr) 6.20 0.00
Total Formulation consumed (ml/Bird) 40.570 0.00
OUTCOME
Mortality DAYS Death % Mortality Death % Mortality
0-10 1 0.14 5 0.61
11-20 1 0.14 11 1.35
21-30 1 0.14 10 1.22
31-40 0 0 5 0.61
TOTAL 3 0.43 31 3.80
Weight Trial period 40 days 40 days
Total number of birds weighed 697 784
Total weight (kg) 1590 1721
Average Weight/bird (kg) 2.28 2.195
Difference in bird weight (g) 86.1
Feed conversion ratio [FCR] (quantity of Feed consumed per unit weight of bird) Trial period 40 days 40 days
Total number of birds weighed 697 784
Total weight (kg) 1590 1721
Quantity of feed consumed (kg) 2815 3056
FCR 1.77 1.78

Example 3:
Trial on broiler birds to understand the efficacy of the formulation containing natural extracts -
A supplementation trial was carried out to understand the efficacy of the formulation containing natural extracts in broiler chickens and performance was observed till 40 days of age. A total of 1,730-1-day old chicks obtained from a commercial hatchery, from 1 to 40 days of age were used and allocated at random to the experimental treatments. The birds were housed in pens of broilers at stocking density 1.31-1.38 sq feet/bird and equipped with a bell drinker and a feed trough. Komerla broiler breed was used for the study. The control batch contained 1560 birds and treatment batch contained 170 birds.

The chicks were fed with feed (SKM brand Starter and Finisher feeds comprising yellow corn, soybean meal, vitamin premix and trace mineral mix with no added growth promoter or veterinary antibiotics). Starter feeds were fed from 0 to 24 days of age and finisher feeds from 25 to 40 days of age. Vaccines were administered through drinking water on 5th day of age Newcastle disease vaccine (LaSota strain); on 12th day of age Infectious Bursal disease vaccine (live virus) and on 21st day of age Newcastle disease vaccine (LaSota strain). Regular antibiotics and liver tonics were given only to the control batch. Treatment batch was exempted from antibiotics and liver tonics. Birds were subjected to 24 hr light throughout the trial. Birds had free access to feed and drinking water.

Normal drinking water at neutral pH was fed to control batch and drinking water containing the natural extracts formulation of present invention (5ppm) fed to the treatment batch. Quantity of the water consumed by both batches was measured on daily basis.
Observations included growth, body weight, feed intake, feed efficiency, general health, drinking water intake and percent of mortality. Also, the meat quality and antibiotics content in meat and liver were studied.

The health of the birds was considered normal throughout the study, and no adverse events were noted in treatment batch. Significant differences were observed in body weight of birds between the groups studied.

There was no death between 0-40 days in treatment batch. There were 12 deaths between 1-10 days, 8 deaths between 11-20 days, 10 deaths between 21-30 days and 6 deaths between 31-40 days in control batch.

The mortality percentage was 0% in treatment batch and 2.38% in control batch. Water consumption was 6.67Liter/bird and 6.64Liter/bird in treatment and control batch respectively. FCR was 1.77 and 1.80kgs in treatment and control batch respectively.
Mortality
No mortality occurred during the study in treatment group (0-40 days). A total of 36 mortalities in control group (2.38%) was seen during the period (0-40 days).
Body Weight
The final average body weight of the control group was 2.19 kg/bird within 40 days. The treatment group significantly increased final body weight within 40 days and the average body weight was 2.28 kg/bird.

Trial No: ZM/02/2017-18
GENERAL INFORMATION
Observations Treatment batch Control batch
Number of Birds 170 1560
Date started 24th Nov 2017 24th Nov 2017
Date finished 2nd Jan 2018 2nd Jan 2018
Total number of days 40 40
Antibiotic given None All regular
Other medicine None Liver tonic given for all days
Average WBT(°C) 25.55 25.55
Area Utilisation (Sq feet/ bird) 1.38 1.31
Average pH of drinking water 7.08 7.08
Quantity of water consumed (Ltr/Bird) 6.77 6.64
Dosage of Formulation applied (ml/Ltr) 7.00 0.00
Total Formulation consumed (ml/Bird) 47.355 0.00
OUTCOME
Mortality DAYS Death % Mortality Death % Mortality
0-10 0 0 12 0.76
11-20 0 0 8 0.513
21-30 0 0 10 0.641
31-40 0 0 6 0.385
TOTAL 0 0 36 2.308
Weight Trial period 40 days 40 days
Total number of birds weighed 170 1524
Total weight (kg) 388 3341
Average Weight/bird (kg) 2.28 2.192
Difference in bird weight (g) 90.1
Feed conversion ratio [FCR] (quantity of Feed consumed per unit weight of bird) Trial period 40 days 40 days
Total number of birds weighed 170 1524
Total weight (kg) 388 3341
Quantity of feed consumed (kg) 685 6025
FCR 1.77 1.80

The antibiotic content was measured in the treatment batch. The liver sample of male and female birds was collected, packed and immediately frozen. The samples were analysed using Charm’s multipurpose Liquid Scintillation Counter (LSC) and Luminometer System and Charm’s Rapid One Step Assay (ROSA) Reader. The antibiotic tested included Chlortetracycline, Oxytetracycline, Tetracycline, Doxycycline, Sulfathiazole, Sulfachlorpyridazine, Sulfamethoxine, Enrofloxacine and Ciprofloxacin. The antibiotic content in all tested samples was either zero or not detected.

The liver samples of broiler chicken taken from local market showed the following antibiotic content - 100 ppb (Sulfonamides) in male bird and 100 ppb (Tetracycline) in female bird.

Example 4:
Trial on broiler birds to understand the efficacy of the formulation containing natural extracts -
A supplementation trial was carried out to understand the efficacy of the formulation containing natural extracts in broiler chickens and performance was observed until 40 days of age. A total of 1,600-1-day old chicks obtained from a commercial hatchery, from 1 to 40 days of age were used and allocated at random to the experimental treatments. The birds were housed in pens at stocking density 1.38–1.40sq feet/bird and equipped with a bell drinker and a feed trough. Komerla broiler breed was used for the study. Total 1,600 chicks were gaged in 7 days combined and separated into two batches. The control batch contained 1112 birds and treatment batch contained 488 birds.

The chicks were fed with feed (SKM brand Starter and Finisher feeds comprising yellow corn, soybean meal, vitamin premix and trace mineral mix with no added growth promoter or veterinary antibiotics). Starter feeds were fed from 0-24 days and finisher feeds from 25-40 days of age. Vaccine was administered on 5th day of age Newcastle disease vaccine (LaSota strain); on 12th day of age Infectious Bursal disease vaccine (live virus) and on 21st day of age Newcastle disease vaccine (LaSota strain). All regular antibiotics and liver tonics were given to the control batch. Treatment batch was exempted from antibiotics and liver tonics. Birds were subjected to 24hr light throughout the trial. Birds had access to feed and drinking water.

Normal drinking water at neutral pH was fed to control batch and drinking water with the natural extract formulation (5ppm) was fed to the treatment batch. Quantity of the water consumed by both the batches was measured on daily basis.

Observations included growth, body weight, feed intake, feed efficiency, general health, drinking water intake and percent of mortality. Also, the meat quality and antibiotics content in meat and liver were studied.

The health of the birds was considered normal throughout the study, and no adverse events were noted in treatment batch. Significant differences were observed in body weight of birds between the groups studied.

There was 1 death between 0-40 days in treatment batch. There were 7 deaths between 1-10 days, 16 deaths between 11-20 days, 15 deaths between 21-30 days and 8 deaths between 31-40 days in control batch.
The mortality percentage was 0.20% in treatment batch and 4.16% was in control batch. Water consumption was 6.71Liter/bird and 6.63Liter/bird in treatment and controlled batch respectively. FCR was 1.66 and 1.64 kg in treatment and control batch respectively.
Mortality
1 mortality occurred during the study in treatment group (0-40 days). A total of 46 mortalities in control group (4.16%) during the period (0-40 days).
Body Weight
The final average body weight of the controls was 2.36 kg/bird within 40 days. The treatment group significantly increased final body weight within 40 days. The average body weight was per bird was 2.44 kg.

TRIAL No: ZM/C/2017-18
GENERAL INFORMATION
Observations Treatment batch Control batch
Number of Birds 488 1112
Date started 1st Aug 2017 1st Aug 2017
Date finished 8th Sep 2017 8th Sep 2017
Total number of days 39 39
Antibiotic given None All regular
Other medicine None Liver tonic given for all days
Average WBT(°C) 29.05 29.05
Area Utilisation (Sq feet/ bird) 1.38 1.40
Average pH of drinking water 7.08 7.08
Quantity of water consumed (Ltr/Bird) 6.71 6.63
Dosage of Formulation applied (ml/Ltr) 7.60 0.00
Total Formulation consumed (ml/Bird) 50.981 0.00
OUTCOME
Mortality DAYS Death % Mortality Death % Mortality
0-10 0 0 7 0.629
11-20 0 0 16 1.439
21-30 1 0.2 15 1.349
31-40 0 0 8 0.719
TOTAL 1 0.2 46 4.137
Weight Trial period 39 days 39 days
Total number of birds weighed 487 1066
Total weight (kg) 1187 2510
Average Weight/bird (kg) 2.437 2.355
Difference in bird weight (g) 82.8
Feed conversion ratio [FCR] (quantity of Feed consumed per unit weight of bird) Trial period 39 days 39 days
Total number of birds weighed 487 1066
Total weight (kg) 1187 2510
Quantity of feed consumed (kg) 1970 4115
FCR 1.66 1.64

Example 5:
Trial on broiler birds to understand the efficacy of the formulation containing natural extracts -
Komerla broiler birds were used for the trials. 1 day old chicks were obtained from a commercial hatchery and allocated at random to the control and treatment batches. The birds were housed in pens with a stocking density of 1.29-1.31 sq feet/ bird. The chicks were observed until 40 days of age. Birds were subjected to 24hr light throughout the trials and were given access to feed and drinking water.

The chicks were fed with feed (SKM brand Starter and Finisher feeds comprising yellow corn, soybean meal, vitamin premix and trace mineral mix with no added growth promoter or veterinary antibiotics). Starter feeds were fed from 0 to 24 days of age and finisher feeds from 25 to 40 days of age. Vaccines were administered through drinking water in the following order-on 5th day of age Newcastle disease vaccine (LaSota strain); on 12th day of age Infectious Bursal disease vaccine (live virus) and on 21st day of age -Newcastle disease vaccine (LaSota strain).

Regular antibiotics and liver tonics were given only to the control batch.
Normal drinking water at neutral pH was fed to the control batch. The formulation containing natural extracts of present invention in a concentration of 5ppm, was dissolved in drinking water and fed to the treatment batch.

Quantity of water consumed by birds of both the batches was measured on daily basis.
Observations included growth, body weight, feed intake, feed efficiency, general health, drinking water intake, mortality rate, meat quality and antibiotics content in meat and liver.

There was 3 death between 0-40 days in treatment batch. There were 3 deaths between 1-10 days, 8 deaths between 11-20 days, 9 deaths between 21-30 days and 3 deaths between 31-40 days in control batch.

The mortality percentage was 0.31% in treatment batch and 4.22% in control batch. FCR was 1.60 and 1.73 kg in treatment and control batch respectively.

TRIAL No: ZM/01/2017-18
GENERAL INFORMATION
Observations Treatment batch Control batch
Number of Birds 970 545
Date started 9th Sep 2017 9th Sep 2017
Date finished 19thOct 2017 19thOct 2017
Total number of days 41 41
Antibiotic given None All regular
Other medicine None Liver tonic given for all days
Average WBT(°C) 29.55 29.55
Area Utilisation (Sq feet/ bird) 1.29 1.31
Average pH of drinking water 6.98 6.98
Quantity of water consumed (Ltr/Bird) 7.40 7.22
Dosage of Formulation applied (ml/Ltr) 7.50 0.00
Total Formulation consumed (ml/Bird) 55.470 0.00
OUTCOME
Mortality DAYS Death % Mortality Death % Mortality
0-10 0 0 3 0.550
11-20 2 0.21 8 1.468
21-30 1 0.10 9 1.651
31-40 0 0 3 0.550
TOTAL 3 0.31 23 4.220
Weight Trial period 41 days 41 days
Total number of birds weighed 967 522
Total weight (kg) 2321 1215
Average Weight/bird (kg) 2.40 2.328
Difference in bird weight (g) 72.6
Feed conversion ratio [FCR] (quantity of Feed consumed per unit weight of bird) Trial period 41 days 41 days
Total number of birds weighed 967 522
Total weight (kg) 2321 1215
Quantity of feed consumed (kg) 3720 2100
FCR 1.60 1.73

It will be readily apparent to one skilled in the art that varying substitutions and modifications may be made to the invention disclosed herein. Thus, it should be understood that although the present invention has been specifically disclosed by the preferred embodiments and optional features, modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, and such modifications and variations are considered to be falling within the scope of the invention.

It is to be understood that the phraseology and terminology used herein is for the purpose of description and should not be regarded as limiting.

FOR ARISHINA LIFE SCIENCES PVT. LTD.

(APEKSHA SAWANT)
IN/PA-1995

We claim:
1. A natural extract formulation comprising extracts of Onion (Allium cepa), Garlic (Allium sativum), Lilac tassel flower (Emilia sonchifolia), Malabar nut (Adathoda vasica), Indian borage (Plectranthus amboinicus), Turmeric (Curcuma longa), Nutmeg (Myristica fragrans) & Black pepper (Piper nigrum).

2. The natural extract formulation of claim 1, comprises Onion (Allium cepa): 22-28%, Garlic (Allium sativum): 22-28%, Lilac tassel flower (Emilia sonchifolia): 12-18%, Malabar nut (Adathoda vasica): 12-18%, Indian borage (Plectranthus amboinicus): 12-18%, Turmeric (Curcuma longa): 1-3%, Nutmeg (Myristica fragrans): 0.1-0.5% and Black pepper (Piper nigrum): 0.05-0.15%.

3. The natural extract formulation of claim 1 and 2, includes organic and aqueous solvent extracts.

4. The natural extract formulation of claim 1, further comprises a water soluble additive.

5. The natural extract formulation of claim 1 and 4, wherein the water soluble additive is Polysorbate 80.

6. The natural extract formulation of claim 1, 4 and 5, wherein the water soluble additive is in a concentration ranging from 1-2%.

7. The natural extract formulation of any of the preceeding claims, is a water soluble formulation.

8. The natural extract formulation is administered to poultry in a concentration from about 1ppm to about 15 ppm.

9. The natural extract formulation of claim 8, is administered to poultry in a preferable concentration from about 5ppm to about 10ppm.

10. The natural extract formulation of any of the preceeding claims is formulated in the form of solution, powder, granules, water dispersible tablet.

11. The natural extract formulation of any of the preceeding claims is provided to the poultry by dissolving in drinking water.

12. A method of improving overall poultry health, immunity, laying bird egg count and decreasing bird mortality using the natural extract formulation comprising extracts of Onion (Allium cepa), Garlic (Allium sativum), Lilac tassel flower (Emilia sonchifolia), Malabar nut (Adathoda vasica), Indian borage (Plectranthus amboinicus), Turmeric (Curcuma longa), Nutmeg (Myristica fragrans) and Black pepper (Piper nigrum).

13. The natural extract formulation of any of the preceeding claims prevents poultry diseases such as chronic respiratory disease, osteoarthritis and jaundice, helps in promoting poultry weight gain, improves the quality and flavor of meat and is free of any antibiotic residue.
Dated this 1st day of July, 2018
FOR ARISHINA LIFE SCIENCES PVT. LTD.


(APEKSHA SAWANT)
IN/PA-1995
NATURAL EXTRACTS FORMULATION FOR IMPROVING POULTRY HEALTH

Abstract of the invention:
The present invention relates to a water soluble formulation derived from natural sources for improving the overall poultry health and number of eggs in laying birds. More particularly, the invention relates to a water soluble formulation derived from herbs and spices for poultry health and eggs count in laying birds.