[Claim 1] An anti-human NGF antibody Fab5 fragment comprising:
a heavy-chain variable region consisting of an amino acid sequence shown by SEQ
IDNO:6;and
a light-chain variable region consisting of an amino acid sequence shown by SEQ
IDNO:4.
[Claim 2] The Fab' fragment according to Claim 1, wherein a heavy-chain constant
region of the Fab5 fragment is a human Igyl constant region.
[Claim 3] The Fab5 fragment according to Claim 1, wherein a light-chain constant
region of the Fab' fragment is a human IgK constant region.
[Claim 4] The Fab' fragment according to Claim 1, wherein the heavy-chain
constant region of the Fab' fragment is the human Igyl constant region, and the light-chain constant region of the Fab3 fragment is the human IgK constant region.
[Claim 5] The Fab' fragment according to Claim 1, comprising;
a heavy-chain fragment consisting of an amino acid sequence shown by SEQ ID NO:10, SEQ ID NO:14, or SEQ ID NO:16; and
a light chain consisting of an amino acid sequence shown by SEQ ID NO: 12.
[Claim 6] The Fab' fragment according to any one of Claims I to 5, wherein the Fab'
fragment is conjugated to polyethylene glycol
[Claim 7] A polynucleotide comprising a sequence that encodes the heavy-chain
fragment of the Fab' fragment according to Claim 1.
[Claim 8] A polynucleotide comprising a sequence that encodes the light chain of
the Fab' fragment according to Claim 1.
[Claim 9] An expression vector comprising the polynucleotide according to Claim 7
and/or Claim 8=
[Claim 10] A host cell transformed with the expression vector according to Claim 9.

[Claim 11] The host cell according to Claim 105 which is selected from a group
consisting of the following (a) and (b)s
(a) a host cell transformed with an expression vector comprising a polynucleotide comprising a sequence that encodes the heavy-chain fragment of the Fab' fragment according to Claim 1 and a polynucleotide comprising sequence that encodes the light chain of the Fab3 fragment; and
(b) a host cell transformed with an expression vector comprising a polynucleotide comprising a sequence that encodes the heavy-chain fragment of the Fab' fragment according to Claim 1 and with an expression vector comprising a polynucleotide comprising a sequence that encodes the light chain of the Fab' fragment.
[Claim 12] Amethodof producing the anti-human NGF antibody Fab5 fragment according to Claim 1, comprising expressing an anti-human NGF antibody Fab' fragment by culturing the host cell according to Claim 10 or 11.