NOVEL HUMAN LXRα VARIANTS
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority to provisional U.S. Application Serial
No. 60/496007, filed on August 18, 2003, which is herein incorporated by reference in its
entirety.
FIELD OF THE INVENTION
[0002] The present invention relates to novel liver X receptors (LXR) and nucleic
acid sequences encoding such receptors.
BACKGROUND OF THE INVENTION
[0003] Gene expression is regulated in eukaryotic cells by the interplay of
transcription factors. Steroid hormones (e.g., glucocorticoids, mineralocorticoids,
estrogens, progestins, androgens and vitamin D) were found to bind to their nuclear
receptors which are transcription factors and by this means regulate expression of gene
coding for specific proteins and control critical cellular activities such as differentiation,
proliferation and apoptosis (Meier, Recept. Signal Transduct. Res. 1997,17, 319-335).
The liver X receptors (LXRs) are a family of transcription factors that were first identified
as orphan members of the nuclear receptor superfamily. The identification of a specific
class of oxidized derivatives of cholesterol as ligands for the LXRs has been crucial to
helping understand the function of these receptors in vivo and first suggested their role in
the regulation of lipid metabolism. LXRs, members of the nuclear receptor super-family,
include LXRα (also termed RLD-1) and ubiquitous receptor (UR, also called LXR0).
LXR-dependent pathways include but are not limited to cholesterol-7a!pha-hydroxylase
to increase the consumption of cholesterol via the bile acid route, expression of ABC
proteins with the potential to stimulate reverse cholesterol transport and increase plasma
HDL-C levels (Venkateswaran eta!., J. Biol. Chem. 275, 2000,14700-14707; Costet et
al., J. Biol. Chem. 2000 275(36):28240-28245; Ordovas, Nutr. Rev. 58, 2000, 76-79,
Schmitz and Kaminsky, Front. Biosci. 6, 2001, D505-D514), and/or inhibit intestinal
cholesterol absorption (Mangelsdorf, Xllth Internationa! Symposium on Atherosclerosis,
Stockholm, June 2000). In addition, possible cross talk between fatty acid and
cholesterol metabolism mediated by liver LXR have been hypothesized (Tobin et a!.,
Mol. Endocrinol. 14, 2000, 741-752).

[0004] In summary, ongoing research suggests that there exists complexity in
LXR-dependent pathways and LXR variants may contribute to these pathways
differently.
[0005] In order to understand the LXR-dependent pathways and mechanism of
LXR action, it is important to isolate and characterize novel subtypes, variants, and/or
isoforms of the LXR. Identification of the underlying LXR subtype, variant, or isoform
responsible for a particular disease state or pathological condition can permit a more
accurate means of prognosticating the LXR-related disease outcomes. Furthermore, the
presence or amount of expression of such polynucleotides and/or the polypeptides
encoded by such polynucleotides can be used for diagnosing associated pathological
conditions, diagnosing a susceptibility to an associated pathological condition; develop
gene-specific and isoform-specific therapies for diseases or disorders influenced by LXR,
follow the progress of a therapy for an LXR-related disease or disorder, and/or develop
new pharmaceutical drug targets.
[0006] With the recognition that these variants can be as critical to metabolic and
physiologic function as proteins that are separately encoded, there is a need to identify
and to characterize additional variants of the LXRα proteins. The present invention
satisfies this need and provides related advantages as well.
SUMMARY OF THE INVENTION
[0007] The invention relates to the identification of nucleic acid sequences
encoding novel LXRα variants (e.g., LXRα-64, LXRα-42e+, and LXRoc-42e-) and certain
activities and features of those variants. Accordingly, the invention relates to an isolated
nucleic acid molecule encoding a human liver X receptor alpha (LXRα) variant
polypeptide such as an isolated nucleic acid molecule encoding SEQ ID NO:4,6, 8,17,
or 19, an isolated nucleic acid molecule encoding an amino acid sequence having at
least 90% (e.g., 90%, 95%, or 99%) identity with the SEQ ID NO:4, 6, 8,17, or 19, an
isolated nucleic acid molecule that hybridizes with the isolated nucleic acid molecule of
described above under hybridization conditions of 6X SSC (1M NaCI), 50 % formamide,
1 % SDS at 42 °C, and a wash in 1X SSC at 42°C, and a wash at 68°C, in 0.2XSSC, and
0.1% SDS; and an isolated nucleic acid molecule that is complementary to any of the
LXRα variant sequences described herein. The LXRα variant nucleic acid molecule can
also be a fragment of a full length LXRα variant mRNA or cDNA. In general, at least a
portion of the fragment is sequence that is not found in a wild type LXRα mRNA or

cDNA. In some embodiments, the isolated nucleic acid molecule consists of SEQ ID
NO:3, 5, 7, 16, or 18.
[0008] In certain embodiments, the isolated nucleic acid molecule is a DNA
molecule. The isolated nucleic acid molecule can be an RNA molecule, or can contain
synthetic nucleotides and naturally occurring nucleotides. In some cases, the isolated
nucleic acid molecule includes the nucleic acid sequence of SEQ ID NO:3, 5, 7,16, or 18
or a fragment thereof, or can consist of the nucleic acid sequence of SEQ ID NO:3, 5, 7,
16, or 18. In certain embodiments, a nucleic acid molecule of the invention can encode
a polypeptide that has LXR-responsive pathway activity, e.g., can form a dimer with a
wild-type LXRα, can form a heterodimer with a retinoid X receptor (RXR) (e.g., an RXRa,
RXRp, or RXRy), or can affect the expression or activity of an LXR-responsive pathway
molecule such as expression of ABCA1 or SREBP-1C.
[0009] In another embodiment, the invention relates to a polypeptide (an LXRα
variant polypeptide, e.g., an LXRα-64 polypeptide, an LXRα-42e+ polypeptide, an LXRα-
42e- polypeptide, or a fragment thereof) encoded by an isolated LXRα variant nucleic
acid molecule described herein. In some cases, the polypeptide can form a dimer with a
wild-type LXRα. In some cases, the polypeptide can form a heterodimer with an RXR
(e.g., an RXRa, RXRp or RXRy). Formation of the heterodimer can, in certain
embodiments, inhibit formation of a heterodimer between the RXR and a nuclear
receptor with which the RXR naturally heterodimerizes. In this case, the formation of the
heterodimer can result in modulation (e.g., a decrease or increase) of an activity
associated with dimerization of the RXR and the nuclear receptor with which it naturally
heterodimerizes. In another embodiment, an LXRα variant polypeptide can form a
heterodimer that inhibits formation of an RXR homodimer. In some cases, the inhibition
results in modulation (e.g., an increase or decrease) of an activity induced by the RXR
homodimer. In certain embodiments, an LXRα variant polypeptide or fragment thereof
can exhibit dominant negative activity with respect to an LXR (e.g., a wild type LXRα). In
certain embodiments, the polypeptide described herein is a fragment of an LXRα variant
and can exhibit at least one function of an LXRα variant, e.g., binding to an antibody that
specifically binds to the LXRα variant.
[0010] Also included in the invention is a construct (e.g., a plasmid, including
without limitation, pCMV/myc, pcDNA 3.1, or a derivative thereof) that includes an

isolated nucleic acid molecule of an LXRα variant or a fragment thereof. The isolated
nucleic acid molecule can be operatively linked to a regulatory sequence.
[0011] In another embodiment, the invention relates to a host cell comprising an
isolated nucleic acid molecule as described herein (e.g., an LXRα variant or a derivative
thereof) or a descendent of the cell. Also included is host cell comprising a construct
described supra. The host cell can be a prokaryotic cell (e.g., an E. co//cell), or an
eukaryotic cell such as a mammalian cell, e.g., a mouse cell, rat cell, monkey cell, or
human cell (such as a human embryonic cell or other type of stem cell). Examples of
host cells, without limitation include a human hepatoma cell (HepG2), a Chinese hamster
ovary cell (CHO), a monkey COS-1 cell, and a human embryonic kidney cell (HEK 293).
Other examples of host cells include, without limitation, a Saccharomyces cerevisiae cell,
a Schizosaccharomyces pombe cell, and a Pichia pastoris cell.
[0012] In one aspect the invention is an isolated LXRα variant polypeptide that
includes the amino acid sequence of an LXRα-64, LXRα-42e+, or and LXRα-42e-, e.g.,
the isolated polypeptide includes the amino acid sequence of SEQ ID NO:4, 6, 8,17, 19,
a naturally-occurring allelic variant thereof, or a fragment thereof. The isolated
polypeptide can consist of the amino acid sequence of SEQ ID NO:4, 6, 8, 17,19, or a
fragment thereof. In general, a fragment does not share homology with more than 25
contiguous amino acids of SEQ ID NO:2 (e.g., 20, 15,10, or 5 contiguous amino acids).
In certain embodiments, the isolated LXRα variant polypeptide includes heterologous
amino acid sequences.
[0013] In another aspect, the invention relates to a method for detecting the
presence of an LXRα variant polypeptide (e.g., an LXRα-64, LXRα-42e+, or LXRα-42e-)
in a sample. The method includes contacting the sample with a compound (e.g., an
antibody such as a monoclonal antibody) that selectively binds to an LXRα variant
polypeptide (or a fragment thereof) and determining whether the compound binds to the
polypeptide in the sample. The invention also includes a kit that includes a compound
that selectively binds to an LXRα variant polypeptide (e.g., an LXRα-64, LXRα-42e+, or
LXRα-42e-) and instructions for use.
[0014] An embodiment of the invention includes an antibody that specifically
binds to an isolated LXRα variant polypeptide described herein (e.g., an LXRα-64,
LXRα-42e+, or LXRα-42e-), or a fragment thereof. In some cases, the antibody does
not bind significantly to wild type LXRα. The antibody is, in certain embodiments, a

polyclonal antibody. In other embodiments, the antibody is a monoclonal antibody. The
antibody can include a detectable label. Also included is a fragment of an antibody such
as a Fab fragment of an antibody that specifically binds to an LXRα variant. The
invention also relates to a composition that includes an antibody described herein or a
fragment thereof and a pharmaceutically acceptable carrier.
[0015] An aspect of the invention includes a method of identifying a new LXRα
variant nucleic acid molecule (e.g., an LXRoc-64, LXRα-42e+, or LXRαe-). The method
includes hybridizing a sample comprising one or more nucleic acid molecules with an
LXRα variant nucleic acid molecule or a fragment thereof under stringent hybridization
conditions, identifying a nucleic acid molecule in the sample that hybridizes with the
LXRα variant nucleic acid molecule, thereby identifying a putative LXRα variant nucleic
acid molecule, and determining the sequence of the putative LXRα variant nucleic acid
molecule, wherein a putative LXRα variant nucleic acid molecule having a sequence that
is not identical to the sequence of an LXRα variant is a new LXRα variant nucleic acid.
In some cases, the new LXRα variant nucleic acid molecule encodes a known LXRα
variant polypeptide. In some cases the new LXRα variant nucleic acid molecule
encodes an LXRα polypeptide that is not identical to a known LXRα variant (e.g., an
LXRα-64, LXRα-42e+, or LXRαe-). A new LXRα variant polypeptide can include one or
more conservative substitutions compared to a known LXRα variant polypeptide.
[0016] In one aspect, the invention relates to a method of detecting expression of
an LXRα variant (e.g., an LXRα-64, LXRα-42e+, or LXRα-42e-) in a biological sample.
The method includes hybridizing the biological sample with an LXRα variant nucleic acid
molecule or fragment thereof (as described herein) and determining whether the nucleic
acid molecule hybridizes to a nucleic acid molecule in the sample, wherein hybridization
indicates that the LXRα variant is expressed. In some embodiments, the amount of
hybridization is determined (e.g., an absolute amount or a relative amount compared to a
control or reference amount).
[0017] Another aspect of the invention relates to a method of decreasing RXR
dimer formation in a cell. The method includes contacting the cell with an LXRα variant
polypeptide (e.g., an LXRα-64, LXRα-42e+, or LXRα-42e-) or fragment thereof, thereby
inhibiting RXR dimer formation (e.g., RXR heterodimerization is inhibited or RXR
homodimerization is inhibited).

[0018] In yet another aspect the invention relates to a method of identifying an
LXRoc variant (e.g., an LXRoc-64, LXRα-42e+, or LXRoc-42e-) ligand. The method
includes providing a sample comprising an LXRα variant polypeptide, contacting the
sample with a test compound, determining whether the test compound can bind to the
LXRα variant, such that a compound that can bind to the LXRα variant is an LXRα
variant ligand. In some embodiments, the Kd of the ligand is less than 1 x 106,less than
1 x 109, between 1 X106 and 1 X 1012, between 1 x 109 and 1 X 1012. In some cases, an
RXR is present in the sample. The method can include determining whether the LXRα
variant ligand can bind a wild type LXRα, e.g., determining that the LXRα variant ligand
does not bind to a wild type LXRα. In some cases, the identified LXRα variant ligand
has a higher affinity for an LXRα variant compared to a wild type LXRα.
[0019] An aspect of the invention relates to modulating (e.g., increasing or
decreasing) the expression of an LXRα-regulated gene. The method includes
modulating expression or activity of an LXRα variant (e.g., an LXRα-64, LXRα-42e+. or
LXRα-42e-). Examples of the LXRα-regulated gene include, without limitation, an
SREBP-1C (sterol regulatory binding element 1c), FAS, CYP7A1 (cholesterol 7-alpha
hydroxylase), ApoE, CETP (cholesterol ester transfer protein), LPL (lipoprotein lipase),
ABCA1 (ATP-binding cassette transporter-1), ABCG1, ABCG5, ABCG8, ABCG4, and
PLTP (phospholipid transfer protein).
[0020] In yet another aspect, the invention relates to a method of modulating
LXRα variant (e.g., an LXRα-64, LXRα-42e+, or LXRα-42e-) expression or activity in a
subject. The method includes introducing into a subject an LXRα variant nucleic acid
molecule or a fragment thereof in an amount and for a time sufficient for the LXRα
variant to be expressed and modulate LXRα expression or activity. In some
embodiments the LXRα variant inhibits expression or activity (e.g., induction of
expression of an LXRα-dependent pathway gene) of a wild-type LXRα. In some cases,
the activity is LXRα heterodimerization, e.g., ligand-stimulated heterodimerization.
[0021] In another aspect, the invention includes a method of modulating
expression or activity of an RXR in a subject. The method includes introducing into a
subject an LXRα variant (e.g., an LXRα-64, LXRα-42e+, or LXRα-42e-) nucleic acid
molecule or a fragment thereof in an amount and for a time sufficient for the LXRα
variant to be expressed and modulate expression or activity of the RXR. In some
embodiments, heterodimerization of the RXR (e.g., heterodimerization of RXR with a

PPARα, PPARγ, PPARδ, RAR, XR, or PXR) is modulated (e.g., inhibited) or
homodimerization of the RXR is modulated (e.g., inhibited). The RXR can be, e.g., an
RXRa, RXRp, or RXRy.
[0022] In yet another aspect, the invention includes a method for treating an
individual having an RXR-related disease or disorder, the method comprising
administering to the individual a pharmaceutically effective amount of an LXRα variant
(e.g., an LXRα-64, LXRoc-42e+, or LXRαe-) or a fragment thereof.
[0023] The invention also relates to a pharmaceutical composition that includes a
cell that can express an LXRα variant (e.g., an LXRoc-64, LXRoc-42e+, or LXRαe-) or
fragment thereof, and optionally, includes a pharmaceutically acceptable carrier; an
isolated LXRα variant nucleic acid molecule or fragment thereof as described herein and
a pharmaceutically acceptable carrier; or an LXRα variant (e.g., an LXRα-64, LXRα-
42e+, or LXRαe-) polypeptide as described herein and a pharmaceutically acceptable
carrier.
[0024] Unless otherwise defined, all technical and scientific terms used herein
have the same meaning as commonly understood by one of ordinary skill in the art to
which this invention belongs. Although methods and materials similar or equivalent to
those described herein can be used in the practice or testing of the present invention,
suitable methods and materials are described below. All publications, patent
applications, patents, and other references mentioned herein are incorporated by
reference in their entirety. In addition, the materials, methods, and examples are
illustrative only and not intended to be limiting.
[0025] Other features and advantages of the invention will be apparent from the
detailed description, drawings, and from the claims.
BRIEF DESCRIPTION OF THE
DRAWINGS AND SEQUENCE DESCRIPTIONS
[0026] The invention can be more fully understood from the following detailed
description, the drawings, and sequences, which form a part of this application.
[0027] Fig. 1A depicts a sequence comparison of wild type LXRα (native) cDNA
with a portion of the variant LXRα-64 cDNA (referred to in Example 2). The top line
illustrates a portion of the wild type LXRα sequence and the bottom line depicts portions
of the LXRα-64 sequence. The numbers represent the nucleotide position from the start
codon of each cDNA sequence.

[0028] Fig. 1B depicts a sequence comparison of the predicted amino acid
sequences of human LXRα (native) with LXRα-64 corresponding to the sequences in
Fig. 1 A. The top line depicts a portion of the native LXRα amino acid sequence and the
bottom line is a portion of the LXRα -64 amino acid sequence. The numbers represent
the amino acid positions in the predicted sequences. The additional sequence that is
specific for the LXRα-64 variant is underlined.
[0029] Fig. 2A depicts a sequence comparison of a portion of wild type LXRα
cDNA with a portion of the novel variant LXRα-42e+ cDNA (referred to in Example 2).
The top line depicts portions of the native LXRα sequence and the bottom line is a
portion of the LXRα-42e+ sequence. The numbers represent the nucleotide positions
from the start codon of the cDNAs.
[0030] Fig. 2B depicts a sequence comparison of the predicted amino acid
sequences of a human LXRα (wild type) with LXRα-42e+. The top line is a portion of the
native LXRα sequence and the bottom line is a portion of the new variant. The numbers
represent the amino acid positions. Sequence that is specific for the LXRα-42e+ variant
is underlined.
[0031] Fig. 3A depicts a sequence comparison of a portion of a wild type LXRα
cDNA with a portion of the novel variant LXRα-42e- cDNA (referred to in Example 2).
The top line depicts portions of the wild type LXRα sequence and the bottom line is a
portion of the LXRα-42e- sequence. The numbers represent the nucleotide positions
from the start codon of the cDNAs.
[0032] Fig. 3B depicts a sequence comparison of the predicted amino acid
sequences of a wild type human LXRα with LXRα-42e-. The top line is a portion of the
wild type LXRα sequence and the bottom line is a portion of the new variant. The
numbers represent the amino acid positions. Sequence that is specific for the LXRα-
42e- variant is underlined.
[0033] Figure 4 is a diagrammatic representation of LXRα-64 mRNA.
[0034] Figure 5 is a diagrammatic representation of LXRα-42e+ mRNA.
[0035] Figure 6 is a diagrammatic representation of LXRα-42e"mRNA.
[0036] Fig. 7A is a bar graph depicting the results of experiments assaying the
relative RNA expression of LXRα-64 in various tissues.

[0037] Fig. 7B is a bar graph depicting the results of experiments assaying the
relative RNA expression of LXRα-42 (LXRα-42e+ and LXRα-42e" combined) in various
tissues.
[0038] Fig. 8A is a bar graph depicting the results of experiments assaying gene
regulation of RNA expression of LXRα-64 in THP-1 cells.
[0039] Fig. 8B is a bar graph depicting the results of experiments assaying gene
regulation of RNA expression of LXRα-42 in THP-1 cells.
[0040] Fig. 9 is a bar graph depicting the results of experiments assaying LXRα-
64 and LXRα-42 inhibition of LXR ligand-dependent activation of a reporter gene.
[0041] Fig. 10 is a bar graph depicting the results of experiments assaying the
inhibition of LXR ligand-dependent activation of a reporter gene by LXRα-64 and LXRα-
42. The difference between this experiment and the experiment whose results are
shown in Fig. 9 is that 293 cells were cotransfected with the wild type LXRoc and each of
the new variants simultaneously .
[0042] Fig. 11 is a bar graph depicting the results of experiments assaying
SREBP-1C expression in HEK293 cells transfected with expression vectors encoding
RXRa (RXRa), wild type LXRoc (LXRα) and RXRa, or LXRα-64 (L64) and RXRoc in the
presence or absence of an LXRα agonist (TO901317), an RXRa agonist (9RA), or both
agonists. Samples are RXRa + pCMV (control vector), RXRa + L64, RXRa + LXRα.
Expression is displayed as a fold change compared to control.
[0043] Fig. 12 is a bar graph depicting the results of experiments assaying
ABCA1 expression in HEK 293 cells transfected with expression vectors encoding RXRa
(RXRa), wild type LXRα (LXRα) and RXRa, or LXRα-64 (L64) and RXRa in the
presence or absence of an LXRα agonist (TO901317), an RXRa agonist (9RA), or both
agonists. Samples are RXRa + pCMV (control vector), RXRa + L64, RXRa + LXRα.
Expression is displayed as a fold change compared to control.
[0044] A brief list of sequence descriptions is provided below and sequences are
provided after the Examples and in the figures.
SEQ ID NO:1 is the nucleotide sequence that codes for the wild type LXRα.
SEQ ID NO:2 is the deduced amino acid sequence of wild type LXRα
SEQ ID NO:3 is the nucleotide sequence that codes for the variant, LXRα-64.
SEQ ID NO:4 is the deduced amino acid sequence of variant, LXRα-64.
SEQ ID NO:5 is the nucleotide sequence that codes for the variant, LXRα-42e+.

SEQ ID NO:6 is the deduced amino acid sequence of variant, LXRα-42e+.
SEQ ID NO:7 is the nucleotide sequence that codes for the variant, LXRα-42e~.
SEQ ID NO:8 is the deduced amino acid sequence of variant, LXRα-42eSEQ ID NO:9 is the nucleotide sequence of the forward primer LXRα-For.
SEQ ID NO:10 is the nucleotide sequence of the reverse primer LXRα-rev.
SEQ ID NO:11 is the nucleotide sequence of the forward primer L64-for.
SEQ ID NO:12 is the nucleotide sequence of the reverse primer L64-rev.
SEQ ID NO:13 is the nucleotide sequence of the L64 TaqMan probe.
SEQ ID NO:14 is part of LXRα promoter sequence used for the iuciferase assay
(referred to in Example 6)
SEQ ID NO:15 is the nucleotide sequence of the LXR response element (LXRE).
SEQ ID NO:16 is the unique nucleotide sequence of LXRα-64 variant which
contains additional sequence compared to the wild type that connects exons 6
and 7 of wild type LXRα, creating a longer exon 6 in LXRα-64 variant. The new
exon 6 includes all of exon 6 as described for wild-type LXRα in addition to extra
sequence that is derived from sequence in intron 6 of wild type LXRα that is
located between exon 6 and exon 7.
SEQ ID NO:17 is the deduced amino acid sequence encoded by SEQ ID NO:16.
SEQ ID NO:18 is the unique nucleotide sequence of LXRα-42e that combines
with exon 8 of wild type LXRα to create a longer exon 8 in the LXRα-42 variant.
This sequence is 234 nucleotides in length and contains a stop codon (TAG) at
position 126, thus the following 108 nucleotides are untranslated. It is found in
both LXRα-42e- and LXRα-42e+.
SEQ ID NO:19 is the deduced amino acid sequence encoded by SEQ ID NO:18.
SEQ ID NO:20 is the nucleotide sequence of the LXRα response element
(LXRE) used in the present invention.
SEQ ID NO:21 is the nucleotide sequence of the primer L42-For.
SEQ ID NO:22 is the nucleotide sequence of the primer L42-Rev.
SEQ ID NO:23 is the nucleotide sequence of an L42 probe.
SEQ ID NO:24 is a portion of the nucleotide sequence of a wild type (native)
LXRα cDNA.
SEQ ID NO:25 is a portion of the nucleotide sequence of an LXRα-64 cDNA.
SEQ ID NO:26 is a portion of the nucleotide sequence of an LXRα-64 cDNA.

SEQ ID NO:27 is a portion of the amino acid sequence of a wild type LXRα
cDNA.
SEQ ID NO:28 is a portion of the amino acid sequence of an LXRα-64 cDNA.
SEQ ID NO:29 is a portion of the nucleotide sequence of a wild type LXRα
cDNA.
SEQ ID NO:30 is a portion of the nucleotide sequence of an LXRα-42e+ cDNA.
SEQ ID NO:31 is a portion of the nucleotide sequence of an LXRoc-42e+ cDNA.
SEQ ID NO:32 is a portion of the amino acid sequence of a wild type LXRα.
SEQ ID NO:33 is a portion of the amino acid sequence of an LXRα-42e+ cDNA.
SEQ ID NO:34 is a portion of the nucleotide sequence a wild type LXRα cDNA.
SEQ ID NO:35 is a portion of the nucleotide sequence an LXRoc-42e- cDNA.
SEQ ID NO:36 is a portion of the nucleotide sequence LXRα-42e- cDNA.
SEQ ID NO:37 is a portion of the nucleotide sequence a wild type LXRα cDNA.
SEQ ID NO:38 is a portion of the nucleotide sequence LXRα-42e- eDNA.
SEQ ID NO:39 is a portion of the amino acid sequence of a wild type LXRα.
SEQ ID NO:40 is a portion of the amino acid sequence of an LXRα-43e-.
DETAILED DESCRIPTION OF THE INVENTION
[0045] Applicants have succeeded in identifying and characterizing new splice
variants of an LXRα gene that encode novel LXRα variants referred to herein as LXRα-
64, LXRα-42e+ and LXRα-42e", respectively. The newly identified sequences produce
variants that differ structurally and functionally from known LXRα proteins. LXRα-64,
LXRα-42e+ and LXRα-42e" variants are encoded by the polynucleotide sequences of
SEQ ID NO:3, SEQ ID NO:5, and SEQ ID NO:7, respectively, and represent alternative
variants of the full-length. LXRα cDNA.
[0046] Genomic organization analysis showed that" the newly isolated variants;
LXRα-64, LXRα-42e+, and LXRα-42e"share certain protein domains and structural
organization with wild type LXRα (Figs. 4, 5, and 6). RT-PCR analysis revealed that the
variant mRNA transcripts of the present invention are most abundant in liver (Figs. 7A
and 7B). More particularly, LXRα-64 is most highly expressed in liver, small intestine,
and pancreas. LXRα-42e+ and LXRα-42e" are most highly expressed in liver. There is
significantly less expression in other tissues. The N-terminal, DNA binding, and hinge
domains of the three LXRα subtypes are identical to the corresponding regions of wild

type LXRcx, whereas the C-terminal domain and the ligand binding domain (LBD) exhibit
some variability. In contrast with wild type LXRα, LXRα-64 variant has an extra 64
amino acids in its ligand binding domain, LXRoc-42e+ has an alternative 42 amino acids
starting at residue 367 of the wild type LXRα sequence and the C terminal from residue
368 to the end of the wild type LXRα (80 amino acids) is not present in this variant, and
therefore lacks a portion of the ligand binding domain that is present in the wild type
LXRα. LXRα-42e' contains 349 amino acids and lacks 60 amino acids that are encoded
by exon 6 of wild type LXRα. Starting at amino acid 237 of LXRα-42", there is 100%
identity for 71 amino acids with the wild type LXRα. This is followed by 42 amino acids
that are completely different from wild type. Like LXRα-42+, the C-terminal of wild type
LXRα is not present in LXRα-42e-.
[0047] It is also demonstrated herein that the novel LXRα variants are functional
in that they can act as dominant negative modulators of wild-type LXRα activity. In
addition, LXRα-64 and LXRα-42e+ and LXRα-42e" variants have been found to be
upregulated by LXR or RXR agonists in human monocyte/macrophage THP-1 cells (Fig.
8). Furthermore, LXR ligand-dependent activation was found to be sharply decreased
when the novel LXRα-64, LXRα-42e+, and LXRα-42e" variants were co-transfected with
a reporter gene (Figs. 9 and 10). Ligand-dependent induction of LXR-dependent
pathway genes was also decreased in the presence of LXRα-64 in the presence of an
LXRα agonist (Figs. 11 and 12), and in some cases, even in the absence of an LXRα
agonist (Fig. 11).
[0048] The three novel LXRα variants have aiso been shown to antagonize the
biological/biochemical activity of a naturally-occurring (wild type) LXRα protein by acting
as dominant negative genes. A portion of an LXRα protein, e.g., a DNA binding domain
(DBD), can also activate, somewhat less efficiently than a wild type LXRα, the
biological/biochemical activities of a wild type LXRα protein.
[0049] Increasing the expression or activity of an LXRα variant (e.g., LXRα-64) is
useful for treating disorders associated with the expression of SREBP-C1. For example,
disrupting the activity of an LXRα, e.g., by overexpressing an LXRα-64 or increasing the
activity of an LXRα-64 that is expressed in a cell (e.g., by administering a compound that
differentially binds to LXRα-64 compared to wild type LXRα) can provide a method of
inhibiting the insulin induction of SREBP-C1, and therefore provides a method of
inhibiting undesirable induction of fatty acid synthesis by insulin. In another example,

overexpressing an LXROG variant (e.g., LXRα-64) or selectively activating an LXRα
variant (for example, with a compound that differentially binds to the LXRα-variant) can
result in inhibition of SREBP-C1, and therefore provides a method of treating
hypertriglyceridemia, which is a condition that is a strong predictor of heart disease. In
i another example, lowered SREBP-C1 expression (by increased expression or activity of
an LXRα variant such as LXRoc-64) can result in lower expression of VLDL-TGs (very
low density lipoprotein triglycerides), a desirable effect in certain disorders such as
diabetes and certain types of hyperlipoproteinemia. Wild type LXR has the effect of
upregulating ABCA1, which is involved in reverse cholesterol transport and it has been
found that an LXRα variant can inhibit basal expression of SREBP-1C, which is involved
in triglyceride synthesis.
[0050] Nuclear receptors that heterodimerize with RXR and activation of these
heterodimers results in increased expression of specific genes. In the case of
undesirable expression of one or more of these genes (e.g., LXR-mediated upregulation
of SREBPIc), then overexpression of an LXRα-64 is beneficial to a subject if expression
of the LXRα variant binds to the RXR, thereby decreasing the availability of the RXR for
heterodimerization and therefore reducing induction undesirable gene expression.
[0051] As more fully described below, the present invention provides isolated
nucleic acids that encode each of the novel variants of LXRα homologues and fragments
thereof. The invention further provides vectors for propagation and expression of the
nucleic acids of the present invention, host cells comprising the nucleic acids and
vectors of the present invention, proteins, protein fragments, and protein fusions of the
present invention, and antibodies specific for all of any one of the variants. The
invention provides pharmaceutical or physiologically acceptable compositions
comprising, the polypeptides, polynucleotides and/or antibodies of the present invention,
as well as, typically, a physiologically acceptable carrier. The present invention
additionally provides diagnostic, investigational, and therapeutic methods based on the
LXRα-64, LXRα-42e+ and LXRα-42e" nucleic acid fragments, polypeptides and
antibodies of the present invention.

Definitions
[0052] The following definitions and abbreviations are provided for the full
understanding of terms and abbreviations used in this specification.
[0053] As used herein and in the appended claims, the singular forms "a", "an",
and "the" include the plural reference unless the context clearly indicates otherwise.
Thus, for example, a reference to "a host cell" includes a plurality of such host cells, and
a reference to "an antibody" is a reference to one or more antibodies and equivalents
thereof known to those skilled in the art, and so forth.
[0054] The abbreviations in the specification correspond to units of measure,
techniques, properties or compounds as follows: "min" means minutes, "h" means
hour(s), "pL" means microliter(s), "mL" means milliliter(s), "mM" means millimolar, "M"
means molar, "mmole" means millimole(s), "kb" means kilobase, "bp" means base
pair(s), and "IU" means International Units.
"Dulbecco's-modified Eagle Medium" is abbreviated DMEM.
"High performance liquid chromatography" is abbreviated HPLC.
"High throughput screening" is abbreviated HTS.
"Open reading frame" is abbreviated ORF.
"Polyacrylamide gel electrophoresis" is abbreviated PAGE.
"Sodium dodecyl sulfate-polyacrylamide gel electrophoresis" is abbreviated SDS-
PAGE.
"Polymerase chain reaction" is abbreviated PCR.
"Reverse transcriptase polymerase chain reaction" is abbreviated RT-PCR.
"Liver X receptor alpha" is abbreviated LXRα.
"Retinoid X receptor" is abbreviated RXR. RXR refers to all RXRs including
RXRa, RXRp, RXRy, and combinations thereof.
"DNA binding domain" is abbreviated DBD.
"Ligand binding domain" is abbreviated LBD.
"Untranslated region" is abbreviated UTR.
"Sodium dodecyl sulfate" is abbreviated SDS.
[0055] In the context of this disclosure, a number of terms shall be utilized. As
used herein, the term "nucleic acid molecule" refers to the phosphate ester form of
ribonucleotides (RNA molecules) or deoxyribonucleotides (DNA molecules), or any
phosphoester analogs, in either single-stranded form, or a double-stranded helix.
Double-stranded DNA-DNA, DNA-RNA and RNA-RNA helices are possible. The term

nucleic acid molecule, and in particular DNA or RNA molecule, refers only to the primary
and secondary structure of the molecule, and does not limit it to any particular tertiary
forms. Thus, this term includes double-stranded DNA found, inter alia, in linear (e.g.,
restriction fragments) or circular DNA molecules, plasmids, and chromosomes. In
discussing the structure of particular double-stranded DNA molecules, sequences may
be described according to the normal convention of giving only the sequence in the 5' to
3' direction along the nontranscribed strand of DNA (i.e., the strand having a sequence
corresponding to the mRNA).
[0056] A "recombinant nucleic acid molecule" is a nucleic acid molecule that has
undergone a molecular biological manipulation, or is derived from a molecule that has
undergone biological manipulation, i.e., non-natuially-occurring nucleic acid molecule.
Furthermore, the term "recombinant DNA molecule" refers to a nucleic acid sequence
that is not naturally-occurring, or can be made by the artificial combination of two
otherwise separated segments of sequence, i.e., by ligating together pieces of DNA that
are not normaiiy continuous. By "recombinantiy produced" is meant production of a non-
naturally-occurring combination, often accomplished by either chemical synthesis
means, or by the artificial manipulation of isolated segments of nucleic acids, e.g., by
genetic engineering techniques using restriction enzymes, ligases, and similar
recombinant techniques as described by, for example, Sambrook et al., Molecular
Cloning, second edition, Cold Spring Harbor Laboratory, Plainview, N.Y.; (1989), or
Ausubel et al., Current Protocols in Molecular Biology. John Wiley & Sons, New York,
NY (1989), and DNA Cloning: A Practical Approach. Volumes I and II (ed. D. N. Glover)
IREL Press, Oxford, (1985).
[0057] In some cases, a recombinant nucleic acid molecule is constructed to
replace a codon with a redundant codon encoding the same or a conservative amino
acid, while typically introducing or removing a sequence recognition site. Alternatively, a
recombinant nucleic acid molecule is designed to join together nucleic acid segments of
desired functions to generate a single genetic entity comprising a desired combination of
functions not found in the common naturally occurring forms of a manipulated sequence.
Restriction enzyme recognition sites can be the target of such artificial manipulations,
but other site-specific targets, e.g., promoters, DNA replication sites, regulation
sequences, control sequences, or other useful features may be incorporated by design.
Examples of recombinant nucleic acid molecules include recombinant vectors, such as
cloning or expression vectors that contain DNA sequences, which are in a 5' to 3'

(sense) orientation or in a 3' to 5' (antisense) orientation. Vectors suitable for making
recombinant vectors (e.g., expression vectors) that include LXRα variant sequences and
fragments thereof are known in the art.
[0058] The terms "polynucleotide," "nucleotide sequence," "nucleic acid,"
"nucleic acid molecule," "nucleic acid sequence," "nucleic acid fragment,"
"oligonucleotide," "gene," "mRNA encoded by a gene" refer to a series of nucleotide
bases (also called "nucleotides") in DNA and RNA, and include any chain of two or more
nucleotides, RNA or DNA (either single or double stranded, coding, complementary or
antisense), or RNA/DNA hybrid sequences of more than one nucleotide in either single
chain or duplex form (although each of the above species may be particularly specified).
[0059] The polynucleotides can be chimeric mixtures or derivatives, or modified
versions thereof, and can be single-stranded or double-stranded. A polynucleotide can
be modified at a base moiety, sugar moiety, or phosphate backbone, for example, to
improve stability of the molecule or alter its hybridization parameters. An antisense
polynucleotide may comprise a modified base moiety which is selected from the group
including, but not limited to, 5-fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil,
hypoxanthine, xanthine, 4-acetylcytosine, 5-(carboxyhydroxylmethyl) uracil, 5-
carboxymethylaminomethyl-2-thiouridine, 5- carboxymethylamino methyluracil,
dihydrouracil, beta-D-galactosylqueosine, inosine, N6-isopentenyladenine, 1-
methylguanine, 1-methylinosine, 2,2- dimethylguanine, 2-methyladenine, 2-
methylguanine, 3-methylcytosine, 5- methylcytosine, N6-adenine, 7-methylguanine, 5-
methylaminomethyluracil, 5- methoxyaminomethyl-2-thiouracil, beta-D-
mannosylqueosine, 5'- methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthio-N6-
isopentenyladenine, wybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-
thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil- 5-oxyacetic acid methylester,
uracil-5-oxyacetic acid, 5-methyl-2- thiouracil, 3-(3-amino-3-N-2-carboxypropyl) uracil,
and 2,6-diaminopurine. A nucleotide sequence typically carries genetic information,
including the information used by cellular machinery to make proteins and enzymes.
These terms include double- or single-stranded genomic and cDNA, RNA, any synthetic
polynucleotide, genetically manipulated polynucleotide, and both sense and antisense
polynucleotides. This includes single- and double-stranded molecules, i.e., DNA-DNA,
DNA-RNA and RNA-RNA hybrids, as well as "protein nucleic acids" (PNAs) formed by
conjugating bases to an amino acid backbone. This also includes nucleic acids
containing modified bases, for example thio-uracil, thio-guanine, and fluoro-uracil, or

containing carbohydrate, or lipids.
[0060] A "genomic DNA" is a DNA strand that has a nucleotide sequence
homologous with a gene. By way of example, a fragment of chromosomal DNA is a
genomic DNA.
[0061] In the context of the present invention, the- following abbreviations for the
commonly occurring nucleic acid bases are used. "A" refers to adenosine, "C" refers to
cytidine, "G" refers to guanosine, "T" refers to thymidine, and "U" refers to uridine.
[0062] Polynucleotides of the invention can be synthesized using methods
known in the art, e.g., by use of an automated DNA synthesizer (such as those that are
commercially available from Biosearch, Applied Biosystems). As examples,
phosphorothioate oligonucleotides can be synthesized by the method of Stein et ai.,
Nucl. Acids Res., 16, 3209, (1988), methylphosphonate oligonucleotides can be
prepared by use of controlled pore glass polymer supports (Sarin et al., Proc. Natl. Acad.
Sci. U.S.A. 85, 7448-7451, (1988).
[0063] A number of methods have been developed for delivering antisense DNA
or RNA to cells, e.g., antisense molecules can be injected directly into a tissue site.
Modified antisense molecules that are designed to target specific cells (e.g., an
antisense nucleic acid linked to a peptide or antibody that can specifically bind to a
receptor or antigen expressed on the target cell surface) can be administered
systemically. An antisense RNA molecule can be generated by in vitro or in vivo
transcription of a DNA sequences encoding the antisense RNA molecule. Such DNA
sequences can be incorporated into a wide variety of vectors that incorporate suitable
RNA polymerase promoters such as the 17 or SP6 polymerase promoters.
Alternatively, antisense constructs that synthesize antisense RNA constitutively or
inducibly, depending on the promoter used, can be introduced stably into cell lines. To
improve intracellular concentrations of the antisense to a level sufficient to suppress
translation of targeted endogenous mRNAs, one may utilize a recombinant DNA
construct in which the antisense oligonucleotide is placed under the control of a strong
promoter. The use of such a construct to transfect target cells will result in the
transcription of sufficient amounts of single-stranded RNAs that will form complementary
base pairs with the endogenous target gene transcripts and thereby prevent translation
of the target gene mRNA. For example, a vector can be introduced in vivo such that it is
taken up by a cell and directs the transcription of an antisense RNA in the cell. Such a
vector can remain episomai or become chromosomally integrated, as long as it can be

transcribed to produce the desired antisense RNA. Such vectors can be constructed by
recombinant DNA technology methods known in the art. Vectors can be plasmid, viral,
or others known in the art that are suitable for replication and expression in mammalian
cells. Expression of a sequence encoding an antisense RNA can be facilitated using
any promoter known in the art to act in mammalian, e.g., human cells. Such promoters
can be inducible or constitutive. Such promoters include, but are not limited to, the
SV40 early promoter region (Bernoist and Chambon, Nature, 290, 304-310, (1981)), the
promoter contained in the 3' long terminal repeat of Rous sarcoma virus (Yamamoto et
al., Cell, 22, 787-797, (1980)), the herpes thymidine kinase promoter (Wagner et al.,
Proc. Natl. Acad. Sci. U.S.A. 78, 1441-1445, (1981)), and the regulatory sequences of
the met allothionein gene (Brinster et al., Nature 296, 39-42, (1982)). Any type of
plasmid, cosmid, yeast artificial chromosome, or viral vector can be used to prepare the
recombinant DNA construct that can be introduced directly into a tissue site.
Alternatively, viral vectors can be used that selectively infect the desired tissue, in which
case administration may be accomplished by another route (e.g., systemically).
[0064] Ribozymes are RNA molecules possessing the ability to specifically
cleave single-stranded RNA in a manner analogous to DNA restriction endonucleases.
Through the modification of nucleotide sequences that encode a ribozyme, it is possible
to engineer molecules that recognize specific nucleotide sequences in an RNA molecule
and cleave it (Cech, JAMA, 260, 3030, (1988)). A major advantage of this approach is
that, because they are sequence-specific, only mRNAs with specific sequences are
inactivated.
[0065] The polynucleotides described herein may be flanked by natural
regulatory (expression control) sequences, or may be associated with heterologous
sequences, including promoters, internal ribosome entry sites (IRES) and other
ribosome binding site sequences, enhancers, response elements, suppressors, signal
sequences, polyadenylation sequences, introns, 5'- and 3'- non-coding regions, and the
like. The nucleic acids can also be modified by other means known in the art. Non-
limiting examples of such modifications include methylation, "caps", substitution of one
or more of the naturally-occurring nucleotides with an analog, and internucleotide
modifications such as, for example, those with uncharged linkages (e.g., methyl
phosphonates, phosphotriesters, phosphoroamidates, or carbamates) and with charged
linkages (e.g., phosphorothioates or phosphorodithioates). Polynucleotides may contain
one or more additional covalently linked moieties, such as, for example, proteins (e.g.,

nucleases, toxins, antibodies, signal peptides, or poly-L-Iysine), intercalators (e.g.,
acridine or psoralen), chelators (e.g., met als, radioactive met als, iron, or oxidative
met als), and alkylators. The polynucleotides may be derivatized by formation of a
methyl or ethyl phosphotriester or an alkyl phosphoramidate linkage. Furthermore, the
polynucleotides herein can also be modified with a label capable of providing a
detectable signal, either directly or indirectly. Exemplary labels include radioisotopes,
fluorescent molecules, biotin, and the like.
[0066] The term "upstream" refers to a location that is toward the 5' end of the
polynucleotide from a specific reference point.
[0067] The terms "base paired" and "Watson and Crick base paired" are used
interchangeably herein to refer to nucleotides that can be hydrogen bonded to one
another by virtue of their sequence identities in a manner like that found in double-helical
DNA with thymine or uracil residues linked to adenine residues by two hydrogen bonds
and cytosine and guanine residues linked by three hydrogen bonds (see Stryer, (1995)
Biochemistry. 4th edition, which disclosure is hereby incorporated by reference in its
entirety).
[0068] The term "exon" refers to a nucleic acid sequence found in genomic DNA
that is predicted (e.g., using bioinformatics) and/or experimentally confirmed to
contribute contiguous sequence to a mature mRNA transcript.
[0069] The terms "branch site" and "3' acceptor sites" refer to consensus
sequences of 3-splice junctions in eukaryotic mRNAs. Almost all introns begin with GU
and end with AG. From the analysis of many exon-intron boundaries, extended
consensus sequences of preferred nucleotides at the 5 and 3 ends have been
established. In addition to AG, other nucleotides just upstream of the 3" splice junction
also are important for precise splicing (i.e., branch site consensus, YNYURAY and 3'
acceptor site, (Y)nNAG G).
[0070] The term "nucleic acid fragment encoding polypeptide" encompasses a
polynucleotide that includes only the coding sequence as well as a polynucleotide that
includes coding sequence and additional coding or non-coding sequence.
[0071] A nucleic acid molecule is "hybridizable" to another nucleic acid molecule,
such as a cDNA, genomic DNA, or RNA, when a single stranded form of the nucleic acid
molecule can anneal to the other nucleic acid molecule under the appropriate conditions
of temperature and solution ionic strength (Sambrook, J. et al. eds., Molecular Cloning:
A Laboratory Manual (2d Ed. 1989) Cold Spring Harbor Laboratory Press, NY. Vols. 1-3

(ISBN 0-87969-309-6). The conditions of temperature and ionic strength determine the
"stringency" of the hybridization. For preliminary screening for homologous nucleic
acids, low stringency hybridization conditions, corresponding to a Tm of 55°, can be
used, e.g., 5x SSC, 0.1% SDS, 0.25% milk, and no formamide; or 30% formamide, 5x
SSC, 0.5% SDS). Moderate stringency hybridization conditions correspond to a higher
Tm, e.g., 40% formamide, with 5x or 6xSCC. High stringency hybridization conditions
correspond to a higher Tm, e.g., 50% formamide, 5x or 6x SSC. In general, high
stringency conditions are hybridization conditions hybridization in 6X SSC (1M NaCI),
50 % formamide, 1 % SDS at 42 °C, followed by washing for 20 minutes in 1X SSC,
0.1 % SDS at 42°C, and then washing three times for 20 minutes each at 68°C in
0.2XSSC, 0.1% SDS. Hybridization requires that the two nucleic acids contain
complementary sequences although, depending on the stringency of the hybridization,
mismatches between bases are possible. The appropriate stringency for hybridizing
nucleic acids depends on the length of the nucleic acids and the degree of
complementation,- variables well known in the art. The greater the degree of similarity or
homology between two nucleotide sequences, the greater the value of Tm for hybrids of
nucleic acids having those sequences. The relative stability (corresponding to higher
Tm) of nucleic acid hybridizations decreases in the following order: RNA:RNA,
DNArRNA, DNA:DNA. For hybrids of greater than 100 nucleotides in length, equations
for calculating Tm have been derived (Sambrook et al. eds., Molecular Cloning: A
Laboratory Manual (2d Ed. 1989) Cold Spring Harbor Laboratory Press, NY. Vols. 1-3.
(ISBN 0-87969-309-6), 9.50-9.51). For hybridization with shorter nucleic acids, i.e.,
oligonucleotides, the position of mismatches becomes more important, and the length of
the oligonucleotide determines its specificity (Sambrook et al. eds., Molecular Cloning: A
Laboratory Manual (2d Ed. 1989) Cold Spring Harbor Laboratory Press, NY. Vols. 1-3.
(ISBN 0-87969-309-6), 11.7-11.8). The Tm of such sequences can also be calculated
and appropriate hybridization conditions determined.
[0072] Nucleic acid molecules described herein include nucleic acid sequences
that hybridize under stringent conditions to the LXRα variant coding sequences
described herein and complementary sequences thereof. For the purposes of this
invention, the term "stringent conditions" means hybridization will occur only if there is at
least 90%, e.g., at least 95% identity between the nucleic acid sequences. Accordingly,
the present invention also includes isolated nucleic fragments that are complementary to
the complete sequences as reported in the accompanying Sequence Listing as well as

those that are at least 95% identical to such sequences, and polynucleotides having
sequences that are complementary to the aforementioned polynucleotides. The
polynucleotides of the present invention that hybridize to the complement of LXRα
variant coding sequences described herein generally encode polypeptides that retain
substantially the same biological function or activity as the mature LXRα polypeptide
encoded by the cDNA of SEQ ID NO:3, SEQ ID NO:5 or SEQ ID NO:7.
[0073] A "substantial portion" of an amino acid or nucleotide sequence is a
sufficient amount of the amino acid sequence of a polypeptide or the nucleotide
sequence of a gene to putatively identify that polypeptide or gene, either by direct
evaluation of the sequence by one skilled in the art, or by computer automated
sequence comparison and identification using an algorithm such as BLAST (Basic Local
Alignment Search Tool; Altschul, S. F., et al., (1993) J. Mol. Biol. 215:403-410; see also
ncbi.nlm.nih.gov/BLAST. In general, a sequence of at least ten, e.g., at least 15, at least
20, at least 25, or at least 30 or more contiguous nucleotides is necessary to putatively
identify a polypeptide or nucleic acid sequence as homologous to a known protein or
gene. Moreover, with respect to nucleotide sequences, gene specific oligonucleotide
probes comprising 15-30 (e.g., 20-30) contiguous nucleotides may be used in sequence-
dependent methods of gene identification (e.g., Southern hybridization) and isolation
(e.g., in situ hybridization of bacterial colonies or bacteriophage plaques). In addition,
short oligonucleotides of 12-25 bases (e.g., 12-20 bases, 15-20 bases) can be used as
amplification primers in PCR in order to obtain a particular nucleic acid fragment
comprising the primers. Accordingly, a "substantial portion" of a nucleotide sequence
comprises enough of the sequence to specifically identify and/or isolate a nucleic acid
fragment comprising the sequence. The present specification teaches partial or
complete amino acid and nucleotide sequences encoding one or more particular LXR
variants. The skilled artisan, having the benefit of the sequences as reported herein,
can use all or a substantial portion of the disclosed sequences for purposes known to
those skilled in this art. Accordingly, the present invention comprises the complete
sequences as reported in the accompanying Sequence Listing, as well as substantial
portions of those sequences as defined above.
[0074] The term "complementary" is used to describe the relationship between
nucleotide bases that are capable to hybridizing to one another. For example, with
respect to DNA, adenosine is complementary to thymine and cytosine is complementary
to guanine.

[0075] "Identity" or "similarity", as known in the art, refers to relationships
between two or more polypeptide sequences or two or more polynucleotide sequences,
as determined by comparing the sequences. In the art, identity also means the degree
of sequence relatedness between polypeptide or polynucleotide sequences, as the case
may be, as determined by the match between strings of such sequences. Both identity
and similarity can be readily calculated by known methods such as those described in:
Computational Molecular Biology, Lesk, A. M., ed., Oxford University Press, New York,
1988; Biocomputing: Informatics and Genome Projects, Smith, D. W., ed., Academic
Press, New York, 1993; Sequence Analysis in Molecular Biology, von Heinje, G.,
Academic Press, 1987; Computer Analysis of Sequence Data, Part I, Griffin, A. M., and
Griffin, H. G., eds., Humana Press, New Jersey, 1994; and Sequence Analysis Primer,
Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991. Methods
commonly employed to determine identity or similarity between sequences include, but
are not limited to those disclosed in Carillo, H. and Lipman, D., SIAM J. Applied Math.
48:1073 (1988). Methods to determine identity and similarity are codified in publicly
available computer programs. Computer program methods to determine identity and
similarity between two or more sequences include, but are not limited to, GCG program
package (Devereux, J., et al., Nucleic Acids Res. 12(1): 387 (1984)), BU\STP, BLASTN,
and FASTA (Paschal, S. F. et al., J. Molec. Biol. 215: 403 (1990)).
[0076] The term "homologous" refers to the degree of sequence similarity
between two polymers (i.e., polypeptide molecules or nucleic acid molecules). The
homology percentage figures referred to herein reflect the maximal homology possible
between the two polymers, i.e., the percent homology when the two polymers are so
aligned as to have the greatest number of matched (homologous) positions.
[0077] The term "percent homology" refers to the extent of amino acid sequence
identity between polypeptides. The homology between any two polypeptides is a direct
function of the total number of matching amino acids at a given position in either
sequence, e.g., if half of the total number of amino acids in either of the sequences are
the same then the two sequences are said to exhibit 50% homology.
[0078] The term "ortholog" refers to genes or proteins that are homologs via
speciation, e.g., closely related and assumed to have common descent based on
structural and functional considerations. Orthologous proteins generally have the same
function and the same activity in different species. The term "paralog" refers to genes or
proteins that are homologs via gene duplication, e.g., duplicated variants of a gene within

a genome. See also, Fritch, W M (1970) Syst. Zool. 19:99-113. The term "ortholog" may
refer to a polypeptide from another species that corresponds to LXRα variant-like
polypeptide amino acid sequence as set forth in SEQ ID NOS:4, 6, 8, 17, or 19. For
example, mouse and human LXRα-like polypeptides are considered to be orthologs of
each other.
[0079] The term "fragment", "analog", and "derivative" when referring to the
polypeptide of the present invention (e.g., SEQ ID NOs:4, 6, 8,17, and 19), can refer to
a polypeptide that retains essentially at least one biological function or activity as the
reference polypeptide. Thus, an analog includes a precursor protein that can be
activated by cleavage of the precursor protein portion to produce an active mature
polypeptide. The fragment, analog, or derivative of the polypeptide described herein
(e.g., SEQ ID NOS:4, 6, 8, 17, and 19) may be one having conservative or non-
conservative amino acid substitution. The substituted amino acid residues may or may
not be encoded by the genetic code, or the substitution may be such that one or more of
the substituted amino acid residues includes a substituent group, is one in which the
polypeptide is fused with a compound such as polyethylene glycol to increase the half-
life of the polypeptide, or one in which additional amino acids are fused to the
polypeptide such as a signal peptide or a sequence such as polyhistidine tag which is
employed for the purification of the polypeptide or the precursor protein. Such
fragments, analogs, or derivatives are deemed to be within the scope of the present
invention.
[0080] "Conserved" residues of a polynucleotide sequence are those residues
that occur unaltered in the same position of two or more related sequences being
compared. Residues that are relatively conserved are those that are conserved amongst
i more related sequences than residues appearing elsewhere in the sequences.
[0081 ] Related polynucleotides are polynucleotides that share a significant
proportion of identical residues.
[0082] Different polynucleotides "correspond" to each other if one is ultimately
derived from another. For example, messenger RNA corresponds to the gene from
which it is transcribed. cDNA corresponds to the RNA from which it has been produced,
such as by a reverse transcription reaction, or by chemical synthesis of a DNA based
upon knowledge of the RNA sequence. cDNA also corresponds to the gene that
encodes the RNA. Polynucleotides also "correspond" to each other if they serve a

similar function, such as encoding a related polypeptide in different species, strains or
variants that are being compared.
[0083] An "analog" of a DNA, RNA or a polynucleotide, refers to a molecule
resembling a naturally-occurring polynucleotide in form and/or function (e.g., in the ability
to engage in sequence-specific hydrogen bonding to base pairs on a complementary
polynucleotide sequence) but which differs from DNA or RNA in, for example, the
possession of an unusual or non-natural base or an altered backbone. See for example,
Uhlmann et al., Chemical Reviews 90,543-584, (1990).
[0084] The term "naturally-occurring", as applied to an object, refers to the fact
that an object may be found in nature. For example, a polypeptide or polynucleotide
sequence that is present in an organism (including bacteria) that may be isolated from a
source in nature and which has not been intentionally modified by man in the laboratory
is naturally occurring. As used herein, the term "naturally-occurring" is used to refer to a
known LXRoc, which is also referred to as "wild type" LXRα. This use of the term should
not be construed to mean that the LXRα variants described herein are not naturally
occurring.
[0085] A "coding sequence" or a sequence "encoding" an expression product,
such as a RNA, polypeptide, protein, or enzyme, is a nucleotide sequence that, when
expressed, results in the production of that RNA, polypeptide, protein, or enzyme, i.e., a
nucleotide sequence can encode an amino acid sequence for a polypeptide or protein,
e.g., enzyme.
[0086] The term "codon degeneracy" refers to divergence in the genetic code
permitting variation of the polynucleotide sequence without affecting the amino acid
sequence of an encoded polypeptide. Accordingly, the present invention relates to any
nucleic acid fragment or the complement thereof that encodes all or a substantial portion
of the amino acid sequence encoding an LXRα-64, LXRα-42e+, or LXRα-42e" protein as
set forth in SEQ ID NOS:4, 6, and 8. The skilled artisan is well aware of the "codon-
bias" exhibited by a specific host cell to use nucleotide codons to specify a given amino
acid. Therefore, when synthesizing a gene for improved expression in a host cell, it is
desirable to design the gene such that its frequency of codon usage approaches the
frequency of preferred codon usage of the host cell.
[0087] The term "encoding" refers to the inherent property of specific sequences
of nucleotides in a polynucleotide, such as a gene, a cDNA, or an mRNA, to serve as
templates for synthesis of other polymers and macromolecules in biological processes

having either a defined sequence of nucleotides (i.e., rRNA, tRNA, and mRNA) or a
defined sequence of amino acids and the biological properties resulting therefrom.
Thus, a gene encodes a protein if transcription and translation of mRNA corresponding
to that gene produces the protein in a cell or other biological system. Both the coding
strand, the nucleotide sequence of which is identical to the mRNA sequence is (usually
provided in sequence listings), and the non-coding strand, used as the template for
transcription of a gene or cDNA, can be referred to as encoding the protein or other
product of that gene or cDNA.
[0088] The polynucleotide of the present invention, can be in the form of RNA or
in the form of DNA, which DNA includes cDNA and synthetic DNA. The DNA may be
single-stranded or double-stranded. If it is single-stranded, it can be the coding strand or
non-coding (antisense) strand. The coding sequence can be identical to the coding
sequence of any one of SEQ ID NOS:3, 5, 7,16,18 or a fragment thereof or may be a
different coding sequence which, as a result of degeneracy or redundancy of the genetic
code, encodes for the same polypeptide as the reference coding sequence, e.g., one of
SEQ ID NOS:3, 5, 7,16,18, or a fragment thereof.
[0089] The present invention includes variants of the herein-above described
polynucleotides described herein that encode fragments, analogs, and derivatives of the
polynucleotides characterized by the deduced amino acid sequence of SEQ ID NOS:4,
6, 8, 17, or 19. The variant of the polynucleotide can be a naturally occurring allelic
variant of the polynucleotide or a non-naturally-occurring variant of the polynucleotide.
[0090] A polynucleotide of the present invention may have a coding sequence
that is a naturally occurring allelic variant of the coding sequence characterized by the
DNA sequence of the SEQ ID NOS:4, 6 or 8,17 and 19.
[0091] The polynucleotide that encodes the mature polypeptide, i.e., an LXRα,
may include only the coding sequence for the mature polypeptide or the coding
sequence for the mature polypeptide and additional sequence such as gene control
sequence, regulatory sequence, or secretory sequence.
[0092] The present invention therefore includes polynucleotides such that the
coding sequence for the mature polypeptide may be operatively linked in the same
reading frame to a polynucleotide sequence that aids in expression and secretion of a
polypeptide from a host cell (e.g., a signal peptide). The polynucleotide may also
encode a precursor protein.

[0093] A polynucleotide of the present invention may also have coding sequence
fused in-frame to a marker sequence, such as hexa-histidine tag (Qiagen Inc.), at either
3' or 5' terminus of the gene, e.g., to allow purification of the polypeptide.
[0094] "Synthetic genes" can be assembled from oligonucleotide building blocks
that are chemically synthesized using procedures known to those skilled in the art.
These building blocks are ligated and annealed to form gene segments that are then
enzymatically assembled to construct the entire gene. "Chemically synthesized", as
related to a sequence of DNA, means that the component nucleotides were assembled
in vitro. Manual chemical synthesis of DNA may be accomplished using well-known
procedures, or automated chemical synthesis can be performed using one of a number
of commercially available machines. Accordingly, the genes can be tailored for optimai
gene expression based on optimization of nucleotide sequence to reflect the codon bias
of the host cell. The skilled artisan appreciates the likelihood of successful gene
expression if codon usage is biased towards those codons favored by the host.
Determining preferred codons can be based on a survey of genes derived from the host
cell where sequence information is available.
[0095] The term "gene" refers to a nucleic acid fragment that expresses a
specific protein, including regulatory sequences preceding (51 noncoding sequences)
and following (31 non-coding sequences) the coding sequence. "Native gene" refers to a
gene as found in nature with its own regulatory sequences. "Chimeric gene" or
"chimeric construct" refers to any gene or a construct, not a native gene, comprising
regulatory and coding sequences that are not found together in nature. Accordingly, a
chimeric gene or chimeric construct may comprise regulatory sequences and coding
sequences that are derived from different sources, or regulatory sequences and coding
sequences derived from the same source, but arranged in a manner different than that
found in nature. "Endogenous gene" refers to a native gene in its natural location in the
genome of an organism. A "foreign" gene refers to a gene not normally found in the host
organism, but which is introduced into the host organism by gene transfer. Foreign
genes can comprise native genes inserted into a non-native organism, or chimeric
genes. A "transgene" is a gene that has been introduced into the genome by a
transformation procedure.
[0096] "Target gene," "target gene sequence," "target DNA sequence," or "target
sequence" refers to a gene where the gene, its RNA transcript, or its protein product is
modulated by a transcription factor. The target sequence may include an intact gene, an

exon, an intron, a regulatory sequence or any region between genes. The target gene
may comprise a portion of a particular gene or genetic locus in the subject's genomic
DNA. "Target gene," as used herein, refers to a differentially expressed gene involved in
LXR responsive pathways. "Differential expression", refers to both quantitative as well
as qualitative differences in a gene's temporal and/or tissue expression patterns.
Examples of LXR target genes are SREBP-1c (sterol regulatory binding element 1c),
FAS, CYP7A1 (cholesterol 7-alpha hydroxylase), ApoE, CETP (cholesterol ester transfer
protein), LPL (lipoprotein lipase), ABCA1 (ATP-binding cassette transporter-1), ABCG1,
ABCG5, ABCG8, ABCG4, and PLTP (phospholipid transfer protein) (Edwards et al.
Vase. Pharmacol. 38, (2002) 249-256). The term "regulatory sequences" refer to
nucleotide sequences located upstream (51 non-coding sequences), within, or
downstream (3' non- coding sequences) of a coding sequence, and which influence),
e.g., transcription, RNA processing, RNA stability, or translation of the associated coding
sequence. Regulatory sequences may include promoters, translation leader sequences, .
introns, and polyadenylation recognition sequences.
[0097] The term "gene control sequence" refers to the DNA sequences required
to initiate gene transcription plus those required to regulate the rate at which initiation
occurs. Thus a gene control sequence may consist of the promoter, where the general
transcription factors and the polymerase assemble, plus all the regulatory sequences to
which gene regulatory proteins bind to control the rate of these assembly processes at
the promoter. For example, the control sequences that are suitable for prokaryotes may -
include a promoter, optionally an operator sequence, and a ribosome binding site.
Eukaryotic cells are known to utilize promoters, enhancers, and/or polyadenylation
signals.
[0098] The term "promoter" refers to a nucleotide sequence capable of
controlling the expression of a coding sequence or functional RNA. In general, a coding
sequence is located 3' to a promoter sequence. The promoter sequence consists of
proximal and more distal upstream elements, the latter elements often referred to as
enhancers. Accordingly, an "enhancer" is a nucleotide sequence that can stimulate
promoter activity and may be an innate element of the promoter or a heterologous
element inserted to enhance the level or tissue-specificity of a promoter. Promoters may
be derived in their entirety from a native gene, or be composed of different elements
derived from different promoters found in nature, or even comprise synthetic nucleotide
segments. It is understood by those skilled in the art that different promoters may direct

the expression of a gene in different tissues or cell types, or at different stages of
development, or in response to different environmental conditions.
[0099] The term "3' non-coding sequences" refer to nucleotide sequences
located downstream of a coding sequence and include polyadenylation recognition
sequences and other sequences encoding regulatory signals capable of affecting mRNA
processing or gene expression. The polyadenylation signal is usually characterized by
affecting the addition of polyadenylic acid tracts to the 3' end of the mRNA precursor.
[00100] The term "translation leader sequence" refers to a nucleotide sequence
located between the promoter sequence of a gene and the coding sequence. The
translation leader sequence is present in the fully processed mRNA upstream of the
translation start sequence. The translation leader sequence may affect processing of
the primary transcript to mRNA, mRNA stability or translation efficiency.
[00101] The term "operatively linked" refers to the association of two or more
nucleic acid fragments on a single nucleic acid fragment so that the function of one is
affected by the other. For example, a promoter is operatively linked with a coding
sequence when it is capable of affecting the expression of that coding sequence (i.e.,
that the coding sequence is under the transcriptional control of the promoter). Coding
sequences can be operatively linked to regulatory sequences in sense or antisense
orientation.
[00102] The term "domain" refers to an amino acid fragment with specific
biological properties. This term encompasses all known structural and linear biological
motifs. Examples of such motifs include but are not limited to helix-turn-helix motifs,
leucine zippers, glycosylation sites, ubiquitination sites, alpha helices, and beta sheets,
signal peptides which direct the secretion of proteins, sites for post- translational
modification, enzymatic active sites, substrate binding sites, and enzymatic cleavage
sites.
[00103] "DNA-binding domain" refers to the portion of any DNA binding protein
that specifically interacts with desoxyribonucleotide strands. A sequence-specific DNA
binding protein binds to a specific sequence or family of specific sequences showing a
high degree of sequence identity with each other.
[00104] The term "LBD" or "ligand-binding domain" refers to the protein domain of
a nuclear receptor, such as a steroid superfamily receptor or other suitable nuclear
receptor as discussed herein, which binds a ligand (e.g., a steroid hormone).

[00105] The term "reporter gene" means any gene that encodes a product whose
expression is detectable and/or quantifiable by physical, immunological, chemical,
biochemical, or biological assays. A reporter gene product may, for example, have one
of the following attributes, without restrictions: a specific nucleic acid chip hybridization
pattern, fluorescence (e.g., green fluorescent protein), enzymatic activity, toxicity, or an
ability to be specifically bound by a second molecule, labeled or unlabeled.
[00106] The term "RNA transcript" refers to the product resulting from RNA
polymerase-catalyzed transcription of a DNA sequence. When the RNA transcript is a
complementary copy of the DNA sequence, it is referred to as the primary transcript or it
may be an RNA sequence derived from post-transcriptional processing of the primary
transcript and is referred to as the mature RNA. "Messenger RNA (rriRNA)" refers to the
RNA that is without introns and can be translated into polypeptides by the cell. "cDNA"
refers to DNA that is complementary to and derived from an mRNA template. The cDNA
can be single-stranded or converted to double-stranded form using, for example, the
Kienow fragment of DNA polymerase I.
[00107] A sequence "complementary" to a portion of an RNA, refers to a
sequence having sufficient complementarity to be able to hybridize with the RNA,
forming a stable duplex; in the case of double-stranded antisense nucleic acids, a single
strand of the duplex DNA may thus be tested, or triplex formation may be assayed. The
ability to hybridize will depend on both the degree of complementarity and the length of
the antisense nucleic acid. The complementarity of an antisense RNA may be with any
part of the specific nucleotide sequence, i.e., at the 5' non-coding sequence, 3' non-
coding sequence, introns, or the coding sequence. Generally, the longer the hybridizing
nucleic acid, the more base mismatches with an RNA it may contain and still form a
stable duplex (or triplex, as the case may be). One skilled in the art can ascertain a
tolerable degree of mismatch by use of standard procedures to determine the melting
point of the hybridized complex.
[00108] "Functional RNA" refers to sense RNA, antisense RNA, ribozyme RNA, or
other RNA that may not be translated but yet has an effect on cellular processes.
[00109] An "anti-sense" copy of a particular polynucleotide refers to a
complementary sequence that is capable of hydrogen bonding to the polynucleotide and
can therefor be capable of modulating expression of the polynucleotide. These are
DNA, RNA or analogs thereof, including analogs having altered backbones, as
described above. The polynucleotide to which the anti-sense copy binds may be in

single-stranded form or in double-stranded form. A DNA sequence linked to a promoter
in an "anti-sense orientation" may be linked to the promoter such that an RNA molecule
complementary to the coding mRNA of the target gene is produced.
[00110] The antisense polynucleotide may comprise at least one modified sugar
moiety selected from the group including but not limited to arabinose, 2-fluoroarabinose,
xylulose, and hexose. In one embodiment, the antisense oligonucleotide may comprise
at least one modified phosphate backbone selected from the group consisting of a
phosphorothioate, a phosphorodithioate, a phosphoramidothioate, a phosphoramidate, a
phosphordiamidate, a methylphosphonate, an alkyl phosphotriester, and a formacet al or
analog thereof.
[00111] The term "sense" refers to sequences of nucleic acids that are in the
same orientation as the coding mRNA nucleic acid sequence. A DNA sequence linked
to a promoter in a "sense orientation" is linked such that an RNA molecule that contains
sequences identical to an mRNA is transcribed. The produced RNA molecule, however,
need not be transcribed into a functional protein.
[00112] A "sense" strand and an "anti-sense" strand when used in the same
context refer to single-stranded polynucleotides that are complementary to each other.
They may be opposing strands of a double-stranded polynucleotide, or one strand may
be predicted from the other according to generally accepted base-pairing rules. Unless
otherwise specified or implied, the assignment of one or the other strand as "sense" or
"antisense" is arbitrary.
[00113] The term "polynucleotide encoding polypeptide" encompasses a
polynucleotide that may include only the coding sequence as well as a polynucleotide
that may include additional coding or non-coding sequence.
[00114] The term "siRNA" or "RNAi" refers to small interfering RNAs and the
process by which they function. siRNAs are capable of causing RNA interference and
can cause post-transcriptional silencing of specific genes in cells, for example, in
mammalian cells (including human cells) and in the body, for example, mammalian
bodies (including humans). The phenomenon of RNA interference is described and
discussed in Bass, Nature, 411, 428-29, (2001); Elbahir et al., Nature, 411, 494-98,
(2001); and Fire et al., Nature, 391, 806-11, (1998), where methods of making interfering
RNA also are discussed. The siRNAs based upon the sequence disclosed herein can
be made by approaches known in the art, including the use of complementary DNA
strands or synthetic approaches. Exemplary siRNAs could have up to 29 bps, 25 bps,

22 bps, 21 bps, 20 bps, 15 bps, 10 bps, 5 bps or any integer thereabout or
therebetween.
[00115] The term "expression", as used herein, refers to the transcription and
stable accumulation of sense (mRNA) or antisense RNA derived from the nucleic acid
fragment of the invention. Expression may also refer to translation of mRNA into a
polypeptide. "Antisense inhibition" refers to the production of antisense RNA transcripts
capable of suppressing the expression of the target protein.
[00116] The term "overexpression" refers to the production of a gene product in
transgenic organisms that exceeds levels of production in normal or non-transformed
organisms. "Co-suppression" refers to the production of sense RNA transcripts capable
of suppressing the expression of identical or substantially similar foreign or endogenous
genes (U.S. Patent No. 5,231,020, incorporated herein by reference).
[00117] The term "altered levels" refers to the production of gene product(s) in
transgenic organisms in amounts or proportions that differ from that of normal or non-
transformed organisms. Over expression of the polypeptide of the present invention
may be accomplished by first constructing a chimeric gene or chimeric construct in
which the coding region is operatively linked to a promoter capable of directing
expression of a gene or construct in the desired tissues at the desired stage of
development. For reasons of convenience, the chimeric gene or chimeric construct may
comprise promoter sequences and translation leader sequences derived from the same
genes. 3' Non-coding sequences encoding transcription termination signals may also be
provided. The instant chimeric gene or chimeric construct may also comprise one or
more introns in order to facilitate gene expression. Plasmid vectors comprising the
instant chimeric gene or chimeric construct can then be constructed. The choice of
plasmid vector is dependent upon the method that will be used to transform host cells.
The skilled artisan is well aware of the genetic elements that must be present on the
plasmid vector in order to successfully transform, select and propagate host cells
containing the chimeric gene or chimeric construct. The skilled artisan will also
recognize that different independent transformation events will result in different levels
and patterns of expression (Jones et al., 1985, EMBO J. 4:2411-2418; De Almeida et al.,
1989, Mol. Gen. Genetics 218:78-86), and thus that multiple events must be screened in
order to obtain lines displaying the desired expression level and pattern. Such screening
may be accomplished by Southern analysis of DNA, Northern analysis of mRNA
expression, Western analysis of protein expression, or phenotypic analysis.

[00118] The terms "cassette" or "expression cassette" refer to a DNA coding
sequence or segment of DNA that codes for an expression product that can be inserted
into a vector at defined restriction sites. The cassette restriction sites are designed to
ensure insertion of the cassette in the proper reading frame. Generally, foreign DNA is
inserted at one or more restriction sites of the vector DNA, and then is carried by the
vector into a host cell along with the transmissible vector DNA. A segment or sequence
of DNA having inserted or added DNA, such as an expression vector, can also be called
a "DNA construct."
[00119] The term "expression system" means a host cell and compatible vector
under suitable conditions, e.g., for the expression of a protein coded for by foreign DNA
carried by the vector and introduced to the host cell. Common expression systems
include E. coli host cells and plasmid vectors, insect host cells and Baculovirus vectors,
and mammalian host cells and vectors.
[00120] The term "transfection" refers to the insertion of an exogenous
poiynucieotide into a host ceil, irrespective of the method used for the insertion, or the
molecular form of the polynucleotide that is inserted. The insertion of a polynucleotide
per se and the insertion of a vector or plasmid comprised of the exogenous
polynucleotide are included. The exogenous polynucleotide may be transcribed and
translated by the cell, maintained as a nonintegrated vector, for example, a plasmid, or
may be stably integrated into the host genome.
[00121] The term "transformed" refers to any known method for the insertion of a
nucleic acid fragment into a host prokaryotic cell. The term "transfected" refers to any
known method for the insertion of a nucleic acid fragment into a host eukaryotic cell.
Such transformed or transfected cells include stably transformed or transfected cells in
which the inserted DNA is rendered capable of replication in the host cell. They also
include transiently expressing cells that express the inserted DNA or RNA for limited
periods of time. The transformation or transfection procedure depends on the host cell .
being transformed. It can include packaging the nucleic acid fragment in a virus as well
as direct uptake of the nucleic acid fragment, such as, for example, electroporation,
lipofection, or microinjection. Transformation and transfection can result in incorporation
of the inserted DNA into the genome of the host cell or the maintenance of the inserted
DNA within the host cell in plasmid form. Methods of transformation are well known in
the art and include, but are not limited to, lipofection, electroporation, viral infection, and
calcium phosphate mediated direct uptake. Transfection methods are known to those in

the art including calcium phosphate DNA co-precipitation (Methods in Molecular Biology,
Vol. 7, Gene Transfer and Expression Protocols, Ed. E. J. Murray, Humana Press
(1991)); DEAE-dextran; electroporation; cationic liposome-mediated transfection; and
tungsten particle-facilitated microparticle bombardment (Johnston, Nature 346:776-777
(1990)). Strontium phosphate DNA co-precipitation (Brash et al., Molec. Cell. Biol.
7:2031-2034 (1987) is an alternative transfection method.
[00122] "Cells," "host cells," or "recombinant host cells" are terms used
interchangeably herein. It is understood that such terms refer not only to the particular
subject cell but also to the progeny or potential progeny of such a cell. Because certain
modifications may occur in succeeding generations due to either mutation or
environmental influences, such progeny may not, be identical to the parent cell, but are
still included within the scope of the term as used herein. The term "recombinant cell"
refers to a cell that contains heterologous nucleic acid, and the term "naturally-occurring
cell" refers to a cell that does not contain heterologous nucleic acid introduced by the
hand of man.
[00123] The cell may be a prokaryotic or a eukaryotic cell. Typical prokaryotic
host cells include various strains of E. coll Typical eukaryotic host cells are mammalian, -
such as Chinese hamster ovary cells or human embryonic kidney 293 cells (HEK 293
cells). The introduced DNA is usually in the form of a vector containing an inserted
piece of DNA. The introduced DNA sequence may be from the same species as the
host cell or a different species from the host cell, or it may be a hybrid DNA sequence,
containing some foreign and some homologous DNA. It is further understood that such
terms refer not only to the particular subject cell but also to the progeny or potential
progeny of such a cell. Because certain modifications may occur in succeeding
generations due to either mutation or environmental influences, such progeny may not,
in fact, be identical to the parent cell, but are still included within the scope of the term as
used herein.
[00124] The term "clone" refers to a population of cells derived from a single cell
or common ancestor by mitosis. A "cell line" refers to a clone of a primary cell that is
capable of stable growth in vitro for several generations.
[00125] The term "cell growth" refers to an increase in the size of a population of
cells.
[00126] The term "cell division" refers to mitosis, i.e., the process of cell
reproduction.

[00127] The term "proliferation" refers to growth and division of cells. "Actively
proliferating" means cells that are actively growing and dividing.
[00128] The term "differentiate" refers to having a different character or function
from the original type of tissues or cells. Thus, "differentiation" is the process or act of
differentiating.
[00129] The term "gene-inducible system" refers to the use of ligands to regulate
gene expression. Several regulatory systems have been developed that utilize small
molecules to induce gene expression (reviewed in Clackson, Curr. Opin. Chem. Biol., 1,
210-218, (1997); Lewandoski, Nat Rev Genet. 2, 743-755, (2001). A gene-inducible
system is a molecular tool which allows for low to undetectable basal expression of a
target gene when the system is not activated and increased expression levels of the
target gene when the system is activated.
[00130] The term "inhibiting cellular proliferation" refers to slowing and/or
preventing the growth and division of cells. Cells may further be specified as being
arrested in a particular cell cycle stage: G1.(Gap 1), S phase (DNA synthesis), G2 (Gap
2) or M phase (mitosis).
[00131] The term "preferentially inhibiting cellular proliferation" refers to slowing
and/or preventing the growth and division of cells as compared to normal cells.
[00132] The term "apoptosis" refers to programmed cell death as signaled by the
nuclei in normally functioning human and animal cells when age or state of cell health
and condition dictates. "Apoptosis" is an active process requiring metabolic activity by
the dying cell, often characterized by cleavage of the DNA into fragments that give a so
called laddering pattern on gels. Cells that die by apoptosis do not usually elicit the
inflammatory responses that are associated with necrosis, though the reasons are not
clear. Cancerous cells, however, are unable to experience, or have a reduction in, the
normal cell transduction or apoptosis-driven natural cell death process. Morphologically,
apoptosis is characterized by loss of contact with neighboring cells, concentration of
cytoplasm, endonuclease activity- associated chromatin condensation and pyknosis, and
segmentation of the nucleus, among others.
[00133] The term "polypeptide" refers to a polymer of amino acids without regard
to the length of the polymer; thus, "peptides," "oligopeptides", and "proteins" are included
within the definition of polypeptide and used interchangeably herein. The term refers to
a naturally occurring or synthetic polymer of amino acid monomers (residues),
irrespective of length, where amino acid monomer here includes naturally occurring
amino acids, naturally occurring amino acid structural variants, or synthetic non-

naturally-occurring analogs that are capable of participating in peptide bonds. This term
also does not specify or exclude chemical or post-expression modifications of the
polypeptides of the invention, although chemical or post-expression modifications of
these polypeptides may be included or excluded as specific embodiments. Therefore,
for example, modifications to polypeptides that include the covalent attachment of
glycosyl groups, acetyl groups, phosphate groups, lipid groups and the like are expressly
encompassed by the term polypeptide. Further, polypeptides with these modifications
may be specified as individual species to be included or excluded from the present
invention. The natural or other chemical modifications, such as those listed in examples
above can occur anywhere in a polypeptide, including the peptide backbone, the amino
acid side-chains and the amino or carboxyl termini. It will be appreciated that the same
type of modification may be present in the same or varying degrees at several sites in a
given polypeptide. Also, a given polypeptide may contain many types of modifications.
Polypeptides may be branched, for example, as a result of ubiquitination, and they may
be cyclic, with or without branching. Modifications include acetylation, acylation, ADP-
ribosylation, amidation, covalent attachment of flavin, covalent attachment of a heme
moiety, covalent attachment of a nucleotide or nucleotide derivative, covalent attachment
of a lipid or lipid derivative, covalent attachment of phosphotidylinositol, cross-linking,
cyclization, disulfide bond formation, demethylation, formation of covalent cross-links,
formation of cysteine, formation of pyroglutamate, formylation, gamma-carboxylation,
glycosylation, GPI anchor formation, hydroxylation, iodination, methylation,
myristoylation, oxidation, pegylation, proteolytic processing, phosphorylation, prenylation, •
racemization, selenoylation, sulfation, transfer-RNA mediated addition of amino acids to
proteins such as arginylation, and ubiquitination. (See, for instance, proteins-structure
and molecular properties, 2nd Ed., T. E. Creighton, W. H. Freeman and Company, New
York (1993); posttranslational covalent modification of proteins, b. c. Johnson, Ed.,
Academic Press, New York, pgs. 1-12,1983; Seifter et al., Meth. Enzymol. 182:626-646,
1990; Rattan et al., Ann. NY Acad. Sci. 663:48-62,1992). Also included within the
definition are polypeptides which contain one or more analogs of an amino acid
(including, for example, non-naturally-occurring amino acids, amino acids which only
occur naturally in an unrelated biological system, or modified amino acids from
mammalian systems), polypeptides with substituted linkages, as well as other
modifications known in the art, both naturally-occurring and non-naturally-occurring. The
term "polypeptide" may also be used interchangeably with the term "protein" or "peptide".
[00134] The term "peptide" refers to any polymer of two or more amino acids,
wherein each amino acid is linked to one or two other amino acids via a peptide bond (-

CONH-) formed between the NH.sub.2 and the COOH groups of adjacent amino acids.
Preferably, the amino acids are naturally occurring amino acids, particularly alpha-amino
acids of the L-enantiomeric form. However, other amino acids, enantiomeric forms, and
amino acid derivatives may be included in a peptide. Peptides include "polypeptides,"
which, upon hydrolysis, yield more than two amino acids. Polypeptides may include
proteins, which typically comprise 50 or more amino acids. The term "oligopeptide"
herein denotes a protein, polypeptide, or peptide having 25 or fewer monomeric
subunits.
[00135] "Variant" refers to a polynucleotide or polypeptide that differs from a
reference polynucleotide or polypeptide, but retains the essential properties thereof. A
typical variant of a polynucleotide differs in nucleotide sequence from the reference
polynucleotide. Changes in the nucleotide sequence of the variant may or may not alter
the amino acid sequence of a polypeptide encoded by the reference polynucleotide.
Nucleotide changes may result in amino acid substitutions, additions, deletions, fusions
and truncations in the polypeptide encoded by the reference sequence, as discussed
below.
[00136] The term "variant(s)" refers to a polypeptide plurality of polypeptides that
differ from a reference polypeptide respectively. Generally, the differences between the
polypeptide that differs in amino acid sequence from reference polypeptide, and the
reference polypeptide are limited so that the amino acid sequences of the reference and
the variant are closely similar overall and, in some regions, may be identical. A variant
and reference polypeptide may differ in amino acid sequence by one or more
substitutions, deletions, additions, fusions and truncations, which may be present in any
combination. A substituted or inserted amino acid residue may or may not be one
encoded by the genetic code. Typical conservative substitutions include Gly, Ala; Val,
lie, Leu; Asp, Glu; Asn, Gin; Ser, Thr; Lys, Arg; and Phe and Tyr. Additionally, a variant
may be a fragment of a polypeptide of the invention that differs from a reference
polypeptide sequence by being shorter than the reference sequence, such as by a
terminal or internal deletion. A variant of a polypeptide of the invention also includes a
polypeptide that retains essentially the same biological function or activity as such
polypeptide e.g., precursor proteins that can be activated by cleavage of the precursor
portion to produce an active mature polypeptide. Moreover, a variant may be (i) one in
which one or more of the amino acid residues are substituted with a conserved or non-
conserved amino acid residue (preferably a conserved amino acid residue) and such

substituted amino acid residue may or may not be one encoded by the genetic code, or
(ii) one in which one or more of the amino acid residues includes a substituent group, or
(iii) one in which the mature polypeptide is fused with another compound, such as a
compound to increase the half-life of the polypeptide (for example, polyethylene glycol),
or (iv) one in which the additional amino acids are fused to the mature polypeptide such
as a leader or secretory sequence or a sequence which is employed for purification of
the mature polypeptide or a precursor protein sequence. A variant of the polypeptide
may also be a naturally occurring variant such as a naturally occurring allelic variant, or it
may be a variant that is not known to occur naturally. Non-naturally-occurring variants of
polynucleotides and polypeptides may be made by mutagenesis techniques or by direct
synthesis. Also included as variants are polypeptides having one or more post-
translational modifications, for instance glycosylation, phosphorylation, methylation, ADP
ribosylation and the like. Embodiments include methylation of the N-terminal amino acid,
phosphorylations of serines and threonines and modification of C-terminal glycines.
Among polypeptide variants in this regard are variants that differ from the
aforementioned polypeptides by amino acid substitutions, deletions or additions. The
substitutions, deletions or additions may involve one or more amino acids. Alterations in
the sequence of the amino acids may be conservative or non-conservative amino acid
substitutions, deletions or additions. All such variants defined above are deemed to be
within the scope of those skilled in the art from the teachings herein and from the art.
[00137] The LXRα variant described herein that is designated LXRα-64 (SEQ ID
NO:4), is homologous to the previously known LXRα in that it contains a DNA binding
domain and a ligand binding domain; however, different from the known LXRα in its
middle part of the sequence in that it contains 64 new amino acids. By virtue of the
partial identity and partial divergence of their amino acid sequences, the variant and the
known homologues may have some functionality in common but may differ in other
functions. For example, wild-type LXRα is known to be a sensor for cellular oxysterols
and, when activated by its agonists, increase the expression of genes that control sterol
and fatty acid metabolism/homeostasis where as LXRα-L64, LXRα-42e+ and LXRα-42e"
function as dominant negative modulators of the wild type LXRα.
[00138] The term "dominant negative polypeptide" means an inactive variant of a
protein, which, by interacting with the cellular machinery, displaces an active protein from
its interaction with the cellular machinery or competes with the active protein, thereby
reducing the effect of the active protein. For example, a dominant negative receptor that

binds a ligand but does not transmit a signal in response to binding of the ligand can
reduce the biological effect of expression of the ligand. Likewise, a dominant negative
catalytically inactive kinase that interacts normally with target proteins but does not
phosphorylate the target proteins can reduce phosphorylation of the target proteins in
response to a cellular signal. Similarly, a dominant negative transcription factor that
binds to a promoter site in the control region of a gene but does not increase gene
transcription can reduce the effect of a normal transcription factor by occupying promoter
binding sites without increasing transcription.
[00139] The term "splice variant" refers to cDNA molecules produced from RNA
molecules initially transcribed from the same genomic DNA sequence but which have
undergone alternative RNA splicing. Alternative RNA splicing occurs when a primary
RNA transcript undergoes splicing, generally for the removal of introns, which results in
the production of more than one mRNA molecule each of them may encode different
amino acid sequences. The term splice variant may also refer to the proteins encoded
by the above cDNA molecules. The splice variant may be partially identical in sequence
to the known homologous gene product. "Splice variants" refer to a plurality of proteins
having non-identical primary amino acid sequence but that share amino acid sequence
encoded by at least one common exon.
[00140] As used herein, the phrase "alternative splicing" and its linguistic
equivalents includes all types of RNA processing that lead to expression of plural protein
isoforms from a single gene; accordingly, the phrase "splice variant(s)" and its linguistic
equivalents embraces mRNAs transcribed from a given gene that, however processed,
collectively encode plural protein isoforms. For example, and by way of illustration only,
splice variants can include exon insertions, exon extensions, exon truncations, exon
deletions, alternatives in the 5' untranslated region ("5' UT") and alternatives in the 3'
untranslated region ("3" UT"). Such 3' alternatives include, for example, differences in
the site of RNA transcript-cleavage and site of poly(A) addition (e.g.,.Gautheret et al., .
Genome Res. 8:524-530 (1998)).
[00141] The term "isolated" means that the material is removed from its original or
native environment (e.g., the natural environment if it is naturally-occurring). Therefore,
a naturally occurring polynucleotide or polypeptide present in a living animal is not
isolated, but the same polynucleotide or polypeptide, separated by human intervention
from some or all of the coexisting materials in the natural system, is isolated. For
example, an "isolated nucleic acid fragment" is a polymer of RNA or DNA that is single-

or double-stranded, optionally containing synthetic, non-natural or altered nucleotide
bases. An isolated nucleic acid fragment in the form of a polymer of DNA may be
comprised of one or more segments of cDNA, genomic DNA or synthetic DNA. Such
polynucleotides could be part of a vector, integrated into a host cell chromosome at a
heterologous site, and/or such polynucleotides or polypeptides could be part of a
composition, and still be isolated in that such vector or composition is not part of the
environment in which it is found in nature.
[00142] The term "purified" does not require absolute purity; rather, it is intended
as a relative definition. Purification of starting material or natural material to at least one
order of magnitude, preferably two or three orders, and more preferably four or five
orders of magnitude is expressly contemplated. Similarly, the term "substantially
purified" refers to a substance, which has been separated or otherwise removed, through
human intervention, from the immediate chemical environment in which it occurs in
Nature. Substantially purified polypeptides or nucleic acids may be obtained or
produced by any of a number of techniques and procedures generally known in the field.
[00143] The term "purified" is further used herein to describe a polypeptide or
polynucleotide of the present invention that has been separated from other compounds
including, but not limited to, polypeptides, polynucleotides, carbohydrates, or lipids. The
term "purified" may be used to specify the separation of monomeric polypeptides of the
invention from oligomeric forms such as homodimers, heterodimers, or trimers. The term
"purified" may also be used to specify the separation of covalently closed (i.e., circular)
polynucleotides from linear polynucleotides. A substantially pure polypeptide or
polynucleotide typically comprises about 50%, preferably 60 to 90% weight/weight of a
polypeptide or polynucleotide sample, respectively, more usually about 95%, and
preferably is over about 99% pure but, may be specified as any integer of percent
between 50 and 100. Polypeptide and polynucleotide purity, or homogeneity, is
indicated by a number of means well known in the art, such as agarose or
polyacrylamide gel electrophoresis of a sample, followed by visualizing a single band
upon staining the gel. For certain purposes, higher resolution can be provided by using
HPLC or other means that are known in the art. As an alternative embodiment,
purification of the polypeptides and polynucleotides of the present invention may be
expressed as "at least" a percent purity relative to heterologous polypeptides and
polynucleotides (DNA, RNA or both). In one embodiment, the polypeptides and
polynucleotides of the present invention are at least; 10%, 20%, 30%, 40%, 50%, 60%,

70%, 80%, 90%, 95%, 96%, 96%, 98%, 99%, or 100% pure relative to heterologous
polypeptides and polynucleotides, respectively. In another embodiment the polypeptides
and polynucleotides have a purity ranging from any number, to the thousandth position,
between 90% and 100% (e.g., a polypeptide or polynucleotide at least 99.995% pure)
relative to either heterologous polypeptides or polynucleotides, respectively, or as a
weight/weight ratio relative to all compounds and molecules other than those existing in
the carrier. Each number representing a percent purity, to the thousandth position, may
be claimed as individual species of purity.
[00144] A protein may be said to be "isolated" when it exists at a purity not found
in nature where purity may be adjudged with respect to the presence of proteins of other
sequence, with respect to the presence of non-protein compounds, such as nucleic
acids, lipids, or other components of a biological cell, or when it exists in a composition
not found in nature, such as in a host cell that does not naturally express that protein.
[00145] The polypeptide and the polynucleotides of the present invention are
preferably provided in an isolated form, and preferably are purified to homogeneity.
[00146] The term "Mature" protein refers to a post-translationally processed
polypeptide; i.e., one from which any pre- or propeptides present in the primary
translation product have been removed. "Precursor" protein refers to the primary
product of translation of mRNA; i.e., with pre- and propeptides still present. Pre- and
propeptides include but are not limited to intracellular localization signals.
[00147] The term "antibody" refers to a polypeptide, at least a portion of which is
encoded by at least one immunoglobulin gene, or fragment thereof, and that can bind
specifically to a desired target molecule. The term includes naturally occurring forms, as
well as fragments and derivatives.
[00148] Fragments may include those produced by digestion with various
proteases, those produced by chemical cleavage and/or chemical dissociation, and
those produced recombinantly, so long as the fragment remains capable of specific
binding to a target molecule. Among such fragments are Fab, Fab', Fv, F(ab)'2, and
single chain Fv (scFv) fragments. Derivatives within the scope of the term include
antibodies (or fragments thereof) that have been modified in sequence, but remain
capable of specific binding to a target molecule, including: interspecies chimeric and
humanized antibodies; antibody fusions; heteromeric antibody complexes and antibody
fusions, such as diabodies (bispecific antibodies), single-chain diabodies, and
intrabodies (see, e.g., Marasco (ed.), Intracellular Antibodies: Research and Disease

Applications, Springer- Verlag New York, Inc. (1998) (ISBN: 3540641513), the disclosure
of which is incorporated herein by reference in its entirety).
[00149] The term "immunoreactive" refers to a polypeptide when it is
"immunologically reactive" with an antibody, i.e., when it binds to an antibody due to
antibody recognition of a specific epitope contained within the polypeptide.
Immunological reactivity may be determined by antibody binding, more particularly by the
kinetics of antibody binding, and/or by competition in binding using as competitor(s) a
known polypeptide(s) containing an epitope against which the antibody is directed. The
techniques for determining whether a polypeptide is immunologically reactive with an
antibody are known in the art. An "immunoreactive" polypeptide may also be
"immunogenic."
[00150] Antibodies can be produced by any known technique, including harvest
from cell culture of native B lymphocytes, harvest from culture of hybridomas,
recombinant expression systems, and phage display.
[00151] A molecule is "antigenic" when it is capable of specifically interacting with
an antigen recognition molecule of the immune system, such as an immunoglobulin
(antibody) or T cell antigen receptor. An antigenic polypeptide contains at least about 5,
and preferably at least about 10, amino acids. An antigenic portion of a molecule can be
that portion that is immunodominant for antibody or T cell receptor recognition, or it can
be a portion used to generate an antibody to the molecule by conjugating the antigenic
portion to a carrier molecule for immunization. A molecule that is antigenic need not be
itself immunogenic, i.e., capable of eliciting an immune response without a carrier. The
portions of the antigen that make contact with the antibody are denominated "epitopes".
[00152] The term "molecular binding partners"--and equivalently, "specific binding
partners"~refer to pairs of molecules, typically pairs of biomolecules, which exhibit
specific binding. Non-limiting examples are receptor and ligand, antibody and antigen,
and biotin to any of avidin, streptavidin, NeutrAvidin™ and CaptAvidin™.
[00153] The term "binding partner" or "interacting proteins" refers to a molecule or
molecular complex which is capable of specifically recognizing or being recognized by a
particular molecule or molecular complex, as for example, an antigen and an antigen-
specific antibody or an enzyme and its inhibitor. Binding partners may include, for
example, biotin and avidin or streptavidin, IgG, and protein A, receptor-ligand couples,
protein-protein interaction, and complementary polynucleotide strands. The term "
binding partner" may also refer to polypeptides, lipids, small molecules, or nucleic acids

that bind to polypeptides in cells. A change in the interaction between a protein and a
binding partner can manifest itself as an increased or decreased probability that the
interaction forms, or an increased or decreased concentration of protein-binding partner
complex. For example, LXRα-64 or LXRα-42 protein may bind with another protein or
polypeptide and form a complex that may result in modulating LXR or RXR activity.
[00154] "Specific binding" refers to the ability of two molecular species
concurrently present in a heterogeneous (inhomogeneous) sample to bind to one
another in preference to binding to other molecular species in the sample. Typically, a
specific binding interaction will discriminate over adventitious binding interactions in the
reaction by at least two-fold, more typically by at least 10-fold, often at least 100- fold;
when used to detect analyte, specific binding is sufficiently discriminatory when
determinative of the presence of the analyte in a heterogeneous (inhomogeneous)
sample.
[00155] The term "dimeric" refers to a specific multimeric molecule where two
protein polypeptides are associated through covalent or non-covalent interactions.
"Dimeric molecule" can be receptors that are comprised of two identical (homodimeric) or;
different (heterodimeric) protein molecule subunits.
[00156] The term "homodimer" refers to a dimeric molecule wherein the two
subunit constituents are essentially identical, for example RXR and RXR. The
"homodimeric complex" refers to a protein complex between two identical receptors (e.g.,
RXR/RXR). The "homodimeric complex" may include dimeric proteins with minor
microheterogeneities that occasionally arise on production or processing of recombinant
proteins. The term "homodimerization" refers to the process by which two identical
subunits (e.g., RXR and RXR) dimerize.
[00157] The term "heterodimer" refers to a dimeric molecule wherein the two
subunit constituents are different, for example RXR and LXR. The term "heterodimeric
complex" refers to a protein complex between any one of the nuclear receptors (e.g.,
RXR and any one of the variants of the present invention, or, RXR and LXR, LXR,
PPARα, PPARγ, PPARδ, RAR, XR, or PXR). The term "heterodimerization" refers to a
process by which two different subunits (e.g., RXR and LXRα-64) dimerize.
[00158] The term "naturally heterodimerizes" refers to a process by which a
molecule (e.g., polypeptide) normally heterodimerizes with different molecules in nature.
For example, polypeptides that naturally heterodimerize with RXR are the nuclear

receptors that normally heterodimerize with RXR in nature such as LXRα, LXR,
PPARα, PPARγ, PPARδ, RAR, XR.and PXR.
[00159] The term "LXR responsive pathway" refers to any one of the pathways
known in the art which involve activation or deactivation of a nuclear receptor (e.g., LXR
or RXR), and which are at-least partially mediated by the LXR.
[00160] The term "signal transduction pathway" refers to the molecules that
propagate an extracellular signal through the cell membrane to become an intracellular
signal. This signal can then stimulate a cellular response. The polypeptide molecules
involved in signal transduction processes may be receptor and non-receptor proteins.
[00161] The term "receptor" refers to a molecular structure within a cell or on the
surface of the cell that is generally characterized by the selective binding of a specific
substance. Exemplary receptors include cell-surface receptors for peptide hormones,
neurotransmitters, antigens, complement fragments and immunoglobulins as well as
cytoplasmic receptors for steroid hormones.
[00162] The term "modulation" refers to the capacity to either enhance or inhibit a
functional property of a biological activity or process, for example, receptor binding or
signaling activity. Such enhancement or inhibition may be contingent on the occurrence
of a specific event, such as activation of a signal transduction pathway and/or may be
manifest only in particular cell types. A "modulator" of a protein refers to a wide range of
molecules (e.g., antibody, nucleic acid fragment, small molecule, peptide, oligopeptide,
polypeptide, or protein) and/or conditions which can, either directly or indirectly, exert an
influence on the activation and/or repression of the protein (e.g., receptor of interest),
including physical binding to the protein, alterations of the quantity or quality of
expression of the protein, altering any measurable or detectable activity, property, or
behavior of the protein, or in any way interacts with the protein or compound.
[00163] The term "inhibit" refers to the act of diminishing, suppressing, alleviating,
preventing, reducing or eliminating, whether partial or whole, a function or an activity. .
For example, inhibition of gene transcription or expression refers to any level of
downregulation of these functions, including complete elimination of these functions.
The term "inhibit" can be applied to both in vitro as well as in vivo systems. As used
herein, the term "inhibitor" or "repressor" refer to any agent that inhibits.
[00164] The term "small molecule" refers to a synthetic or naturally occurring
chemical compound, for instance a peptide or oligonucleotide that may optionally be
derivatized, natural product or any other low molecular weight (typically less than about

5 KD) organic, bioinorganic or inorganic compound, of either natural or synthetic origin.
Such small molecules may be a therapeutically deliverable substance or may be further
derivatized to facilitate delivery.
[00165] The term "inducer" refers to any agent that induces, enhances, promotes
or increases a specific activity, such as lipid metabolism, or LXR molecule expression.
[00166] The term "agent" or "test agent" or "test sample" refers to any molecule or
combination of more than one molecule that is to be tested.
[00167] Examples of agents of the present invention include but are not limited to
peptides, proteins, small molecules, and antibodies. Nucleotide fragments and portions,
as well as antisense embodiments described, above may also serve as agents, if
desired. Agents can be randomly selected or rationally selected or designed. As used
herein, an agent is said to be "randomly selected" when the agent is chosen randomly
without considering the specific interaction between the agent and the target compound
or site. As used herein, an agent is said to be "rationally selected or designed," when
the agent is chosen on a non-random basis that takes into account the specific
interaction between the agent and the target compound or site and/or the conformation
in connection with the agent's action.
[00168] The term "biological sample" is broadly defined to include any cell, tissue,
biological fluid, organ, multi-cellular organism, and the like. A biological sample may be
derived, for example, from cells or tissue cultures in vitro. Alternatively, a biological
sample may be derived from a living organism or from a population of single-cell
organisms. A biological sample may be a live tissue such as liver. The term "biological
sample" is also intended to include samples such as cells, tissues or biological fluids
isolated from a subject, as well as samples present within a subject. That is, the
detection method of the invention can be used to detect LXR variant mRNA, protein,
genomic DNA, or activity in a biological sample in vitro as well as in vivo. For example,
in vitro techniques for detection of LXR variant mRNA include TaqMan analysis, northern
hybridization, and in situ hybridization. In vitro techniques for detection of LXRoc protein
include enzyme-linked immunosorbent assays (ELISAs), western blots,
immunoprecipitations and immunofluorescence, in vitro techniques for detection of LXR
variant genomic DNA include southern hybridizations.
[00169] The term "test sample" refers to a biological sample from a subject of
interest. For example, a test sample can be a cell sample or tissue sample. A "test
sample" and "biological sample" are used interchangeably herein.

[00170] The term "body fluid" refers to any body fluid including, without limitation,
serum, plasma, lymph fluid, synovial fluid, follicular fluid, seminal fluid, amniotic fluid,
milk, whole biood, sweat, urine, cerebro-spinal fluid, saliva, sputum, tears, perspiration,
mucus, tissue culture medium, tissue extracts, and cellular extracts. It may also apply to
fractions and dilutions of body fluids. The source of a body fluid can be a human body,
an animal body, an experimental animal, a plant, or other organism.
[00171] The terms "treatment", "treating", and "therapy" to any process, action,
application, therapy, or the like, wherein a subject, including a human being, is provided
medical aid with the object of improving the subject's condition, directly or indirectly, or
slowing the progression of a condition or disorder in the subject.
[00172] Furthermore, the term "treatment" is defined as the application or
administration of an agent (e.g., therapeutic agent or a therapeutic composition) to a
subject, or an isolated tissue or cell line from a subject, who may have a disease, a
symptom of disease or a predisposition toward a disease, with the purpose to cure, heal,
alleviate, relieve, alter, remedy, ameliorate, improve or affect the disease, the symptoms
of disease or the predisposition toward disease. As used herein, a "therapeutic agent"
refers to any substance or combination of substances that assists in the treatment of a
disease. Accordingly, a therapeutic agent includes, but is not limited to, small molecules,
peptides, antibodies, ribozymes and antisense oligonucleotides.
[00173] Therapeutic agent or therapeutic compositions may also include a
compound in a pharmaceutical^ acceptable form that prevents and/or reduces the
symptoms of a particular disease. For example a therapeutic composition may be a
pharmaceutical composition that prevents and/or reduces the symptoms of a lipid
metabolism disorder. It is contemplated that the therapeutic composition of the present
invention will be provided in any suitable form. The form of the therapeutic composition
will depend on a number of factors, including the mode of administration. The
therapeutic composition may contain diluents, adjuvants and excipients, among other
ingredients.
[00174] The term " therapeutically effective amount" refers to the amount of a
compound or composition of compounds that, when administered to a subject for treating
a disease, is sufficient to effect such treatment for the disease. The "therapeutically
effective amount" will vary, according to parameters known to those in the art, for
example, depending on the compound, the disease, the severity of the disease, and the
age, weight, or sex of the mammal to be treated.

[00175] The term "subject" refers to any mammal, including a human, or non-
human subject. Non-human subjects can include experimental, test, agricultural,
entertainment or companion animals.
[00176] The present invention incorporates by reference methods and techniques
known in the field of molecular and cellular biology. These techniques include, but are
not limited to techniques described in the following publications: Old, R. W. & S. B.
Primrose, Principles of Gene Manipulation: An Introduction To Genetic Engineering (3d
Ed. 1985) Blackwell Scientific Publications, Boston. Studies in Microbiology; V.2:409 pp.
(ISBN 0-632-01318-4), Sambrook, J. et al. eds., Molecular Cloning: A Laboratory
Manual (2d Ed. 1989) Cold Spring Harbor Laboratory Press, NY. Vols. 1-3. (ISBN 0-
87969-309-6); Miller, J. H. & M. P. Calos eds., Gene Transfer Vectors For Mammalian
Cells (1987) Cold Spring Harbor Laboratory Press, NY (ISBN 0-87969-198-0). The DNA
coding for the protein of the present invention may be any one provided that it comprises
the nucleotide sequence coding for the above-mentioned protein of the present
invention.
Nucleic Acid Molecules
[00177] The present invention relates to isolated nucleic acid molecules that
encode three novel LXRα variant proteins (i.e., LXRα-64, LXRα-42e+, and LXRα-42e").
Also included are nucleic acid molecules having at least 90% sequence identity to an
LXRα variant protein or a fragment thereof, degenerate variants of an LXRα variant,
variants that encode an LXRα-64, LXRα-42e+, and LXRα-42e" protein having
conservative or moderately conservative substitutions, cross-hybridizing nucleic acids
(e.g., that hybridize under conditions of high stringency), and fragments thereof.
[00178] The sequences of the present invention are presented, respectively, in
SEQ ID NO:3 (full length nucleotide sequence of LXRα-64, cDNA), SEQ ID NO:4 (full
length amino acid sequence of LXRα-64), SEQ ID NO:5 (nucleotide sequence encoding
the entirety of LXRα-42e+), SEQ ID NO:6 (full length amino acid sequence of LXRα-
42e+), SEQ ID NO:7 (nucleotide sequence encoding the entirety of LXRα-42e"), SEQ ID
NO:8 (full length amino acid sequence of LXRα-42e"), SEQ ID NO:16 (unique nucleotide
sequence of LXRα-64 variant that connects exon 6 and 7 of wild type LXRα and creates
a bigger exon 6 in LXRα-64 variant compared to exon 6 of the wild type LXRα), SEQ ID
NO:17 (deduced amino acid sequence encoded by SEQ ID NO:16), SEQ ID NO:18
(unique nucleotide sequence of LXRα-42e that combines with exon 8 of wild type LXRα

to create a longer exon 8 in LXRα-42e variants compared the exon 8 of wild type LXRct),
and SEQ ID NO:19 (the deduced amino acid sequence encoded by SEQ ID NO:18).
[00179] The nucleic acids of the present invention can be produced by
polymerase chain reaction (PCR). Such reactions are known to one of skill in the art
(U.S. Pat. Nos. 4,754,065; 4,800,159; 4,683,195 and 4,683,202 provide PCR techniques
and methods and these U.S. Patents are hereby incorporated by reference in their
entirety).
[00180] In another embodiment of the present invention, an LXRα-64, LXRα-42e+
or LXRα-42e" nucleic acid molecule is a synthetic nucleic acid or a mimetic of a nucleic
?cid that may have increased bioavailability, stability, potency, or decreased toxicity
compared to a naturally occurring LXRα variant. Such synthetic nucleic acids may have
alterations of the basic A, T, C, G, orU bases or sugars that make up the nucleotide
polymer to as to alter the effect of the nucleic acid.
[00181] LXRα variant and nucleic acid fragments derived from LXRα variants
described herein can be used as reagents in isolation procedures, diagnostic assays,
and forensic procedures. For example, sequences from an LXRα-64, LXRα-42e+ or
LXRα-42e" polynucleotide described herein to which they can hybridize (e.g., under
stringent hybridization conditions) can be detectably labeled and used as a probe to
isolate other sequences. In addition, sequences from an LXRα-64, LXRα-42e+, or LXRα-
42e" polynucleotide can be used to design PCR primers for use in isolation, diagnostic,
or forensic procedures.
[00182] The LXRα-64, LXRα-42e+, and LXRα-42e" nucleic acid molecules
described herein can also be used to clone sequences located upstream of the LXRα
variant sequences on corresponding genomic DNA. Such upstream sequences may be
capable of regulating gene expression, and may include, e.g., promoter sequences,
enhancer sequences, or other upstream sequences that influence transcription or
translation levels. Once identified and cloned, these upstream regulatory sequences can
be used in expression vectors designed to direct the expression of an inserted gene in a
desired spatial, temporal, developmental, or quantitative fashion.
[00183] Sequences derived from polynucleotides described herein can be used to
isolate the promoters of the corresponding genes using chromosome walking
techniques. Chromosome walking techniques are known in the art, e.g., the
GenomeWalker® kit available from BD Biosciences Clontech (Palo Alto, CA), which may
be used according to the manufacturer's instructions.

[00184] Once the upstream genomic sequences have been cloned and
sequenced, prospective promoters and transcription start sites within the upstream
sequences may be identified by comparing the sequences upstream of the
polynucleotides of the inventions with databases containing known transcription start
sites, transcription factor binding sites, or promoter sequences.
[00185] In addition, promoters in the upstream sequences may be identified using
promoter reporter vectors as follows: The expression of a reporter gene is detected
when placed under the control of regulatory active polynucleotide fragment or variant of
the LXRα-64, LXRα-42e+ and LXRα-42e" promoter region located upstream of the first
exon of the LXRα-64, LXRα-42e+, or LXRα-42e" genes. Suitable promoter reporter
vectors, into which the LXRα-64, LXRα-42e+, or LXRα-42e" promoter sequences may be
cloned include pSEAP-Basic, pSEAP-Enhancer, pBgal- Basic, p(3gal-Enhancer, or
pEGFP-1 Promoter Reporter vectors available from Clontech, or pGL2-basic or pGL3-
basic promoterless luciferase reporter gene vector from Promega. Briefly, each of these
promoter reporter vectors include multiple cloning sites positioned upstream of a reporter .
gene encoding a readily assayable protein such as secreted alkaline phosphatase,
luciferase, beta-galactosidase, or green fluorescent protein. The sequences upstream
an LXRα-64, LXRα-42e+, or LXRα-42e" coding region are inserted into the cloning sites
upstream of the reporter gene in both orientations and introduced into an appropriate
host cell. The level of reporter protein is assayed and compared to the level obtained
from a vector that lacks an insert in the cloning site. The presence of an elevated
expression level by the vector containing the insert with respect to the control vector
indicates the presence of a promoter in the insert. In some cases, the upstream
sequences are cloned into vectors that contain an enhancer for increasing transcription
levels from weak promoter sequences. A significant level of expression by the insert-
containing vector above that observed for the vector lacking an insert indicates that a
promoter sequence is present in the inserted upstream sequence. Promoter sequence
within the upstream genomic DNA may be further defined by site directed mutagenesis,
linker scanning analysis, or other techniques familiar to those in the art.
[00186] The strength and the specificity of the promoter of each LXRα-64, LXRα-
42e+ and LXRα-42e" gene can be assessed through the expression levels of a
detectable polynucleotide operatively linked to the LXRα-64, LXRα-42e+, or LXRα-42e"
promoters in different types of cells and tissues. The detectable polynucleotide may be
either a polynucleotide that specifically hybridizes with a predefined oligonucleotide

probe, or a polynucleotide encoding a detectable protein, including LXRα-64, LXRα-42e+
and LXRα-42e" polypeptides or fragments or variants thereof. This type of assay is well
known to those skilled in the art. Some of the methods are discussed in more detail
elsewhere in the application.
[00187] The promoters and other regulatory sequences located upstream of the
polynucleotides of the inventions may be used to design expression vectors capable of
directing the expression of an inserted gene in a desired spatial, temporal,
developmental, or quantitative manner. A promoter capable of directing the desired
spatial, temporal, developmental, and quantitative patterns may be selected using the
results of the expression analysis described herein. For example, if a promoter that
confers a high level of expression in muscle is desired, the promoter sequence upstream
of a polynucleotide of the invention derived from an mRNA that is expressed at a high
level in muscle may be used in the expression vector.
[00188] Furthermore, nucleic acid fragments of the invention may be used to
isolate and/or purify nucleic acids similar thereto using any methods well known to those
skilled in the art including the techniques based on hybridization or on amplification
described in this section. These methods may be used to obtain the genomic DNAs
which encode the mRNAs from which the LXRα-64, LXRα-42e+ and LXRα-42e" cDNAs
are derived, mRNAs corresponding to LXRα-64, LXRα-42e+ and LXRα-42e" cDNAs, or
nucleic acids which are homologous to LXRα-64, LXRα-42e+ and LXRα-42e" cDNAs or
fragments thereof, such as variants, species homologues or orthologs.
[00189] Alternatively the nucleic acid fragments and genes of the present
invention can be used as a reference to identify subjects (e.g., mammals, humans,
patients) expressing decreases of functions associated with these receptors.
Vectors and Host cells
[00190] The present invention relates to the vectors that include polynucleotides of.
the present invention, host cells that genetically engineered with vectors of the present
invention such as cloning vector or expression vector and to the production of
polypeptides of the present invention by recombinant techniques. For example, LXRα-
64, LXRα-42e+ and LXRα-42e" nucleic acid molecule could be linked to a vector. The
vector may be a self-replicating vector or a replicative incompetent vector. The vector
may be a pharmaceutically acceptable vector for methods of gene therapy.

[00191 ] The present invention further relates to a method of production of the
polypeptides of the present invention by expressing polynucleotides encoding the
polypeptides of the present invention in a suitable host and recovering the expressed
products employing known recombinant techniques. The polypeptides of the present
invention can also be synthesized using peptide synthesizers. Host cells can be
engineered with the vectors of the present invention. The host organism (recombinant
host cell) may be any eukaryotic or prokaryotic cell, or multicellular organism. Alternative
embodiments can employ mammalian or human cells, especially embryonic mammalian
and human cells. Suitable host cells include but are not limited to mammalian cells such
as Human Embryonic Kidney cells (HEK293), Human hepatoma cells (HepG2), Chinese
hamster ovary cells (CHO), the monkey COS-1 cell line, the mammalian cell CV-1),
amphibian cells (e.g., Xenopus egg cell). Yeast cells (Saccharomyces cerevisiae,
Schizosaccharomyces pombe, Pichiapastoris), and insect cells. Furthermore, various
strains of E co//(e.g., DH5D. HB101, MC1061) may be used as host cells in particular for
molecular biological manipulation.
[00192] The vectors may be cloning vectors or expression vectors such as in the
form of a plasmid, a cosmid, or a phage or any other vector that is replicable and viable
in the host cell. The engineered host cells can be cultured in conventional nutrient media
modified as appropriate for activating promoters, selecting transformants or amplifying a
polynucleotide of the present invention. The culture conditions such as pH, temperature,
and the like, are those suitable for use with the host cell selected for expression of the
polynucleotide are known to the ordinarily skilled in the art.
[00193] Plasmids generally are designated herein by a lower case "p" preceded
and/or followed by capital letters and/or numbers, in accordance with standard naming
conventions that are familiar to those of skill in the art. The plasmids herein are either
commercially available, publicly available on unrestricted bases, or can be constructed
from available plasmids by routine application of well-known, published procedures.
Additionally, many plasmids and other cloning and expression vectors that can be used
in accordance with the present invention are well known and readily available to those of
skill in the art. Moreover, those of skill readily may construct any number of other
plasmids suitable for use in the invention. The properties, construction and use of such
plasmids, as well as other vectors, in the present invention will be readily apparent to
those of skill from the present disclosure.

[00194] The appropriate DNA sequence may be inserted into the vector by a
variety of the procedures known in the art.
[00195] The DNA sequence in the expression vector may be operatively linked to
an appropriate expression control sequence(s) (promoter) to direct mRNA synthesis.
Such promoters include but are not limited to SV40, human cytomegalovirus (CMV)
promoters (e.g., pCMV/myc vectors, pcDNA 3.1 vector or any form of the pcDNA series),
SP6, T7, and T3 RNA polymerase promoters. The expression vector may also include a
ribosome binding site for translation initiation, a transcription terminator, and an
appropriate sequence for amplifying the expression. The expression vector may also
include one or more selectable marker genes to provide a specific phenotype for the
selection of transformed host cells such as neomycin resistance for eukaryotic ceils or
ampicillin resistance for E. coll.
[00196] The expression vectors may include at least one selectable marker. Such
markers include but are not limited to dihydrofolate reductase or neomycin resistance for
eukaryotic cell culture and tetracycline or ampicillin resistance genes for culturing in E.
coli and other bacteria. Representative examples of appropriate hosts include, but are
not limited to, bacterial cells, such as E. coli, Streptomyces, and Salmonella typhimurium .
cells; fungal cells, such as yeast cells; insect cells such as Drosophila S2 and
Spodoptera Sf9 cells; animal cells such as CHO, Cos, and Bowes melanoma cells; and
plant cells. Appropriate culture media and conditions for the above-described host cells
are known in the art.
[00197] Illustrative examples of vectors for use in bacteria include, but are not
limited to, pA2, pQE70, pQE60 and pQE-9, available from Qiagen (Valencia, CA); pBS
vectors, Phagescript vectors, Bluescript™ vectors, pNH8A, pNH16a, pNH18A, pNH46A,
available from Stratagene (Cedar Creek, TX); and pGEMEX®-1, pGEMEX®-2, PinPoint™
X series, pET-5 series, available from Promega (Madison, Wl). Eukaryotic vectors
include, but are not limited to, pWLNEO, pSV2CAT, pOG44, pXT1, and pSG, available
from Stratagene; and pSVK3, pBPV, pMSG, and pSVL available from Pharmacia. Other
suitable vectors will be apparent to the skilled artisan.
[00198] The gene can be placed under the control of a promoter, ribosome
binding site (for bacterial expression), suitable gene control sequence, or regulatory
sequences so that the DNA sequence encoding the protein is transcribed into RNA in the
host ceil transformed by a vector containing the expression construct. Such promoters
include but are not limited to SV40, human cytomegalovirus (CMV) promoters (e.g.,

pCMV/myc vectors, pcDNA 3.1 vector or any form of the pcDNA series), SP6, T7, and
T3 RNA polymerase promoters. In some cases it may be desirable to add sequences
that cause the secretion of the polypeptide from the host cell, with subsequent cleavage
of the secretory signal.
[00199] For some applications, it is desirable to reduce or eliminate expression of
genes encoding a polypeptide of the present invention. To accomplish this, a chimeric
gene or a chimeric construct designed for co-suppression of the instant polypeptide can
be constructed by linking a gene or gene fragment encoding that polypeptide to a
promoter sequences. Alternatively, a chimeric gene or chimeric construct designed to
express antisense RNA for all or part of the instant nucleic acid fragment can be
constructed by linking the gene or gene fragment in reverse orientation to a promoter
sequences. Either the co-suppression or antisense chimeric genes can be introduced
into desired host cell via transformation wherein expression of the corresponding
endogenous genes are reduced or eliminated.
Polypeptides
[00200] LXRα variant polypeptides are useful for a variety of applications,
including but not limited to producing antibodies (e.g., that specifically bind to an LXRα
variant), modulating LXR wild type activity, and altering fatty acid and cholesterol
metabolism (e.g., by modulating gene expression of enzymes that regulate fatty acid and
cholesterol metabolism in a cell in which the LXRα variant is expressed). LXRα variant
polypeptides are also useful for identifying compounds that differentially bind to LXRα
wild type polypeptides and LXRα variant polypeptides. Such compounds are candidate
compounds for differentially regulating metabolic activities associated with LXRα.
[00201] The polypeptides of the present invention can be produced by growing
suitable host cells transformed by an expression vector described above under
. conditions whereby the polypeptide of interest is expressed. The polypeptides can then
be isolated and purified. Methods purifying proteins from cell cultures are known in the
art and include, but not limited to, ammonium sulfate precipitation, anion or cation
exchange chromatography, and affinity chromatography.
[00202] Cell-free translation systems can also be employed to produce the
polypeptides of the present invention using the RNAs derived from the polynucleotides of
the present invention.

[00203] The polypeptides of the present invention can be produced by growing
suitable host cells transformed by an expression vector (e.g., as described herein) under
conditions whereby the polypeptide of the interest is expressed. The polypeptide may
then be isolated and purified. Methods of the purification of proteins from cell cultures
are known in the art and include but are not limited to ammonium sulfate precipitation,
anion or cation exchange chromatography, and affinity chromatography.
[00204] Cell-free translation systems may also be employed to produce the
polypeptides of the present invention using the RNAs derived from the polynucleotides of
the present invention.
[00205] Large-scale production of cloned LXRα-64, LXRα-42e+, and LXRα-42e"
can enable the screening of large numbers of LXRα-64, LXRα-42e+, and LXRα-42e"
analogs, and can facilitate the development of new or improved agonists and
antagonists for the treatment of lipid metabolism disorders. More specifically, the
screening of large numbers of analogs for LXRα-64, LXRα-42e+, and LXRα-42e" activity
could lead to development of improved drugs affecting lipid metabolism. Lipid
metabolism disorders and conditions include but are not limited to atherosclerosis,
diabetes, obesity, Alzheimer's disease, inflammatory disorders, and
hypercholesterolemia.
[00206] For some applications it is useful to direct a polypeptide described herein
to different cellular compartments, or to facilitate secretion of a polypeptide from the cell.
It is thus envisioned that the chimeric gene described above may be further
supplemented by altering the coding sequence to encode the instant polypeptides with
appropriate intracellular targeting sequences such as transit sequences added and/or
with targeting sequences that are already present removed.
[00207] Furthermore, the polypeptides of the present invention or cells expressing
them can be used as immunogen to prepare antibodies using methods known to those
skilled in the art. For example, a polypeptide encoded by SEQ ID NOS:3, 5, or 7 or a
fragment thereof and/or a polypeptide encoded by SEQ ID NO:16 or 18, or cells
expressing any of the aforementioned polypeptides can be used as immunogens. Of
particular use are antibodies directed against the novel 64 amino acids of LXRα-64,
which are not present in wild type LXRoc. The antibodies can be polyclonal or
monoclonal, and may include chimeric, single chain, and Fab fragments or the products
of a Fab expression library. The antibodies are useful for detecting the polypeptide of
the present invention in situ in cells or in vitro in cell extracts.

[00208] In addition, a polypeptide of the present invention can be used as a target
to facilitate design and/or identification of compounds that may be useful as drugs (e.g.,
candidate compounds). In particular, these compounds may be used to treat diseases
resulting from alterations in pathways such as bile acid synthesis, control of plasma
lipoprotein composition, the transport of cholesterol from peripheral tissues to the liver,
regulation of cell proliferation, differentiation, and apoptosis. In addition, the
polypeptides of the present invention can be used to identify additional targets (e.g., co-
activator or co-repressor proteins) that may influence LXRα. Various uses of the LXRα
variants of the present invention include but are not limited to therapeutic modulation of
pathophysiologic isoprenoid synthetic pathway, cholesterol metabolism, cholesterol
cataboiism, bile acid synthesis, and cell differentiation (e.g., gene delivery approaches,
gene silencing approaches, protein therapeutics, antibody therapeutics), diagnostic
utility, pharmaceutical drug targets, identification of receptor-based agonists or
antagonists, and study of the molecular mechanisms of LXRα action.
[00209] Moreover, in ceiis with low LXRα activity due to phenotypic expression of
endogenous dominant negative LXRα variants of the present invention, gene-silencing
approaches such as antisense, siRNA (small interfering RNA), can be employed as
strategies to induce or stimulate LXRα activity. Additionally, the novel variants of the
present invention may be used to make fusion LXRα variants that may be employed
toward the development of receptor-based agonists and antagonists.
[00210] Furthermore, the novel sequences of the present invention, e.g., SEQ ID
NO:16, and SEQ ID NO:18, can be used to generate a dominant negative regulator of
wild type LXRα. Nucleic acid molecules of SEQ ID NO:16 or 18 or fragments thereof
can be incorporated into any one of the existing variants such as LXRα, and/or other
nuclear receptors. The resulting new polypeptides comprising the amino acid sequence
encoded by SEQ ID NO:16 and 18 or fragments thereof (e.g., the sequences set forth in
SEQ ID NO:17 and 19) can generate a dominant negative regulator of wild type LXRα. .
[00211] The importance of LXRs, and particularly LXRα to the delicate
balance of cholesterol metabolism and fatty acid biosynthesis has led to the
development of modulators of LXRs that are useful as therapeutic agents or
diagnostic agents for the treatment of disorders associated with bile acid and
cholesterol metabolism. The novel dominant negative LXRα variants of the
present invention can be utilized to develop such therapeutic agents or

diagnostic agents. Accordingly, an embodiment of the present invention is a
method of treating a condition characterized by an aberrant or unwanted level of
LXR (e.g., LXRα) expression, in a subject. The method includes providing the
subject with a therapeutically effective amount of an LXRα-64, LXRα-42e+, or
LXRα-42e" protein, homologous proteins, or fragments of an LXRα variant
protein having a desirable activity such as the ability to inhabit an LXRα variant
activity, or any combination thereof that can modulate an LXRα activity. The
proteins may be provided by introducing into LXRα-bearing cells of the subject, a
nucleic acid sequence encoding an LXRα-64, LXRα-42e+, or LXRα-42e" protein,
homologous protein, or fragment, or any combinations thereof under conditions
such that the cells express an LXRα-64, LXRα-42e+, or LXRα-42e- protein,
homologous protein, or fragment thereof resulting in modulation of wild type
LXRα receptor and/or other nuclear receptors that heterodimerize with RXR.
Examples of these receptors include but are not limited to LXRα, LXR, PPARα,
PPARγ, PPARδ, RAR, XR, and PXR.
[00212] Introduction of an LXRα variant nucleic acid into cells of a subject may
comprise a) treating cells of the subject or a cultured cell or tissue suitable for
transplantation into the subject (e.g., a cultured stem cell line, bone marrow cells,
umbilical cord blood cells) ex vivo to insert the nucleic acid sequence into the cells; and
b) introducing the cells from step a) into the subject (e.g., U.S. patent nos. 6,068,836 and
5,506,674).
[00213] The subject may be an animal such as a mammal (e.g., mouse, rat, non-
human primate, dog, goat, or sheep). The mammalian subject can be a human.
[00214] LXRs function as heterodimers with the retinoid X receptors (RXRs).
Moreover, RXRs are unique in their ability to function as both homodimeric receptors
and as heterodimeric partners (e.g., LXRα, LXR, PPARα, PPARγ, PPARδ, RAR, XR;
and PXR (Miyata et al., J. Biol'. Chem., 271 9189-9192,1996)) in multiple hormone
responsive pathways. LXR variants of the present invention, LXR64, LXRα-42e+, and
LXRα-42e" can heterodimerize with RXR. Thus, for example, where LXR64, LXRα-42e+,
and/or LXRα-42e" variants are translated, RXR will heterodimerize with these variants
rather than heterodimerizing with LXRα, LXRß, PPARα, PPARγ, PPARδ, RAR, XR,
and/or PXR, or homodimerizing with itself (RXR). This reduces the pool of RXR
available for heterodimerization with specific nuclear receptors, and/or homodimerizing.

[00215] Therefore, as dominant negative variants, the novel LXRα-64, LXRα-
42e+, and LXRα-42e" of the present invention may be used for targeting specific
receptors such as LXRα, LXRß, PPARα, PPARγ, PPARδ, RAR, XR, or PXR.
Accordingly, dominant negative LXRα variants of the present invention offer utility for
therapeutic modulation of pathophysiologic conditions, diagnosis, risk for developing a
disease, or treatment of a wide variety of disease states in which RXR, LXR, or other
nuclear receptor (e.g., LXRα, LXRß, PPARα, PPARγ, PPARδ, RAR, PXR, XR) mediate
processes associated with the pathophysiologic condition or disease. Examples of such
diseases are atherosclerosis, diabetes, obesity, cancer, and drug metabolism disorders.
[00216] Furthermore, LXRα variants of the present invention can modulate target
gene expression or target gene product activity by interacting with wild-type LXRα
binding partners such as RXR. LXR or RXR activity, as used herein, refers to
modulation of LXR (e.g., LXRα) or RXR target gene expression or activity, respectively.
[00217] In one embodiment, target gene specificity of RXR-containing cells can
be altered by contacting the cells with at least one of the novel LXRα variants of the
present invention. In one specific embodiment, the RXR-containing cell, target genes
operatively associated with response element(s) having the sequence 5'-
AGGTTAnnnnTGGTCA-3' (SEQ ID NO:15), wherein each *'n" is independently selected
from A, G, T or C, can be activated by contacting the cells with at least one of the novel
LXRα variants of the present invention.
[00218] The effect of LXRα variants of the present invention on homodimerization
or heterodimerization processes can be determined using various methods known in the
art. Examples of these methods are described in Terrillon et al. Molecular
Endocrinology 2003, 17:677-691, Germain-Desprez et al., J. Biol. Chem., 2003, 278
(25) 22367-22373, and Mercier et al., J. Biol. chem. 2002, 277 (47) 44925-44931. For
example, the activity of RXR can be determined by quantitative assessment of RXR
homodimerization or heterodimerization using any of the techniques in the above
references. For example, nuclear receptor homo- and heterodimerization can be
quantitated by fusing one of the nuclear receptors (e.g., an RXR) cDNA to the energy
donor Rluc (Renilla luciferase) at the carboxyl terminus and fusing the second nuclear
receptor (e.g., an LXRα) cDNA to the energy acceptor GFP (green fluorescent protein).
Using BRET technology (Biosignal Packard), which allows separation between the
Renilla luciferase and the green fluorescent protein emission spectra, the homo- and
heterodimerization of the nuclear receptors can be quantitated.

[00219] Furthermore, the present invention relates to methods of reducing the
expression of mammalian SREBP-1 genes. The invention is based on the discovery
that LXRα variants, as dominant negatives, inhibit wild-type LXRct and correspondingly
can inhibit SREBP-1 expression in mammalian cells. The latter conclusion can readily
be confirmed by assessing SREBP-1 gene expression in the presence and absence of
the variants of the present invention. Abnormal expression of SREBP-1 gene is involved
in conditions such as lipodystrophy, hyperglyceremia, hypertriglyceridemia and diabetes.
The variants of the present invention are useful not only for therapeutic and prophylactic
treatment of conditions that are mediated by SREBP-1 over-expression, but are also
useful for investigation of the mechanisms of fatty acid homeostasis, and the causes and
mechanisms of lipodystrophy.
Antibodies
[00220] The invention also provides an isolated and purified antibody, e.g., a
monoclonal antibody or polyclonal antibody, including an idiotypic or anti-idiotypic
antibody, which is specific for a novel LXRα variant. The polypeptides of the present
invention or cells expressing them may be used as immunogen to prepare antibodies by
methods known to those skilled in the art. For example, these polypeptides encoded by
SEQ ID NOS:3, 5, 7, 16, or 18 or any portion of SEQ ID NOS:3, 5, 7, 16, or 18 and/or
encoded by SEQ ID NO:3, 5, 7,16, or 18 or cells expressing any of the aforementioned
polypeptides may be used as immunogens. These antibodies can be polyclonal or
monoclonal and may include chimeric, single chain, and Fab fragments or the products
of the Fab expression library. The antibodies are useful for detecting the polypeptide of
the present invention in situ in cells or in vitro in cell extracts.
[00221] - For example, the antibody may specifically recognize the novel 64 amino
acids of the novel variant. Rabbits are immunized with a peptide comprising SEQ ID
NO:4 or an immunogenic portion thereof, or a fusion peptide comprising SEQ ID NO:4,
and polyclonal antisera specific for the novel variants isolated. Alternatively, spleen cells
from immunized animals are fused to myeloma cells to produce hybridomas. The
hybridomas are then screened to identify ones secreting a monoclonal antibody specific
for a polypeptide or peptide comprising the 64 amino acid sequences of the novel LXRα-
64 variant. These antibodies are useful to detect the novel LXRα-64 variants in
biological samples, e.g., clinical samples, to detect the relative amount of the novel
variant to other variant.

Screening Assays
[00222] In general, the new methods described herein include methods of
identifying compounds that can modulate the expression or activity of an LXRoc variant.
In some cases, the compounds are identified that modulate the expression or activity of
an LXRoc variant and either do not affect, or affect to a lesser extent, the expression or
activity of a wild type LXRoc.
[00223] Also included are methods of producing LXRoc (e.g., large-scale
production) of cloned LXRα would enable the screening of relatively large numbers of
LXRα analogs, and would facilitate the development of new or improved agonists and
antagonists in the clinical therapy of -scale production of cloned LXRα would enable the
screening of large numbers of LXRα related disorders such as lipid metabolism
disorders. More specifically, the screening of large numbers of analogs for scale
production of cloned LXRα would enable the screening of large numbers of LXRα
activity could lead to development of improved tools and drugs for use in diagnosis and
clinical therapy of, e.g., lipodystrophy, hypertriglyceridemia, hyperglyceremia, diabetes,
or hypercholesterolemia.
[00224] In one embodiment, the polypeptides of the present invention are used as
targets to facilitate design and/or identification of compounds that modulate the
expression or activity of the polypeptides, e.g., by binding to a polypeptide. Such
compounds are candidate compounds for treating disorders associated with LXRoc-
mediated pathways, e.g., can be used as drugs to regulate one or more aspects of an
LXRα pathway. In particular, such compounds can be used to treat diseases resulting
from alterations in hormone responsive pathways such as diabetes and drug metabolism
disorders. In addition, the polypeptides of the present invention can be used to identify
additional targets (e.g., co-activator or co-repressor proteins) that may influence
hormone signaling. Various uses of the LXRα variants of the present invention include
but are not limited to therapeutic modulation of pathophysiologic conditions involving
aberrant lipid metabolism (e.g., gene delivery approaches, gene silencing approaches,
protein therapeutics antibody therapeutics), diagnostic utility, pharmaceutical drug
targets, identification of receptor-based agonists or antagonists, and study of the
molecular mechanisms of LXRα action.
[00225] The systematic study of LXRα variants will make it possible to deduce
structure-activity relationships for the proteins in question. Knowledge of these variants
with respect to the disease studied is fundamental, since it makes it possible to

understand the molecular cause of the pathology. Furthermore, the novel LXRα variants
may be used for targeting of specific receptor interactions as a distinct approach in
identification of tissue selective nuclear receptor modulators such as LXRα, LXRß,
PPARα, PPARγ. PPARδ, RAR, XR, and PXR.
[00226] Accordingly, the invention provides methods (also referred to herein as
"screening assays") for identifying modulators, i.e., candidate compounds or agents
(e.g., proteins, peptides, peptidomimetics, peptoids, small molecules, or other drugs)
that bind to LXRα variant proteins, have a stimulatory or inhibitory effect on, for example,
LXRα variant expression or activity, or have a stimulatory or inhibitory effect on, for
example, the expression or activity of an LXRα variant substrate. Compounds thus
identified can be used to modulate the activity of target gene products (e.g., LXRα
variant genes) in a therapeutic protocol, to elaborate the biological function of the target
gene product, or to identify compounds that disrupt normal target gene interactions.
[00227] In one embodiment, the invention provides assays for screening
candidate or test compounds that are substrates of an LXRα variant protein or
polypeptide or a biologically active portion thereof. In another embodiment, the invention
provides assays for screening candidate or test compounds that bind to or modulate the
activity of an LXRα variant protein or polypeptide or a biologically active portion thereof.
[00228] The test compounds of the present invention can be obtained, for
example, using any of the numerous approaches in combinatorial library methods known .
in the art, including biological libraries; peptoid libraries (libraries of molecules having the
functionalities of peptides, but with a novel, non-peptide backbone that are resistant to
enzymatic degradation but that nevertheless remain bioactive; see, e.g., Zuckermann et
al. (1994) J. Med. Chem. 37:2678-85); spatially addressable parallel solid phase or
solution phase libraries; synthetic library methods requiring deconvolution; the 'one-bead
one-compound' library method; and synthetic library methods using affinity
chromatography selection. The biological library and peptoid library approaches are
limited to peptide libraries, while the other four approaches are applicable to peptide,
non-peptide oligomer or small molecule libraries of compounds (Lam (1997) Anticancer
Drug Des. 12:145).
[00229] Examples of methods for the synthesis of molecular libraries can be found
in the art, for example in: DeWitt et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90:6909; Erb
et al. (1994) Proc. Natl. Acad. Sci. USA 91.-11422; Zuckermann et al. (1994) J. Med.
Chem. 37:2678; Cho et al. (1993) Science 261:1303; Carrell et al. (1994) Angew. Chem.

Int. Ed. Engl. 33:2059; Carell et al. (1994) Angew. Chem. Int. Ed. Engl. 33:2061; and in
Gallop et al. (1994) J. Med. Chem. 37:1233.
[00230] Libraries of compounds may be presented in solution (e.g., Houghten
(1992) Biotechniques 13:412-421), or on beads (Lam (1991) Nature 354:82-84), chips
(Fodor (1993) Nature 364:555-556), bacteria (Ladner, U.S. Patent NO. 5,223,409),
spores (Ladner, supra), plasmids (Cull et al. (1992) Proc. Natl. Acad. Sci. USA 89:1865-
1869) or on phage (Scott and Smith (1990) Science 249:386-390; Devlin (1990) Science
249:404-406; Cwirla et al. (1990) Proc. Natl. Acad. Sci. 87:6378-6382; Felici (1991) J.
Mol. Biol. 222:301-310; Ladner supra.).
[00231] In one embodiment, an assay is a cell-based assay in which a cell that
expresses an LXRoc variant protein or biologically active portion thereof is contacted with
a test compound, and the ability of the test compound to modulate a LXRα variant
activity is determined. Determining the ability of the test compound to modulate LXRα
variant activity can be accomplished by monitoring, for example, dominant negative
activity of the LXRα variant in a cell expressing a wild type LXRα, e.g., by monitoring the .
expression of an LXRα-inducible gene or gene product. The cell, for example, can be of
mammalian origin, e.g., human.
[00232] The ability of the test compound to modulate LXRα variant binding to a
compound, e.g., a naturally occurring LXRα variant ligand, or to bind to an LXRα variant
can also be evaluated. This can be accomplished, for example, by coupling the
compound with a radioisotope or enzymatic label such that binding of the compound to
the LXRα variant can be determined by detecting the labeled compound in a complex.
Alternatively, an LXRα variant can be coupled with a radioisotope, enzymatic label, or
engineered to include a peptide label to monitor the ability of a test compound to
modulate LXRα variant binding to, e.g., an LXRα variant, wild type LXRα, or
heterodimerize with another member of the steroid receptor superfamily in a complex.
For example, compounds can be labeled with 125l, 35S, 14C, or 3H, either directly or
indirectly, and the radioisotope detected by direct counting of radioemmission or by
scintillation counting. Alternatively, compounds can be enzymatically labeled with, for
example, horseradish peroxidase, alkaline phosphatase, or luciferase, and the enzymatic
label detected by determination of conversion of an appropriate substrate to product.
[00233] The ability of a compound to interact with an LXRα variant, with or without
the labeling of any of the Interactants, can be evaluated. For example, a

microphysiometer can be used to detect the interaction of a compound with an LXRα
variant without the labeling of either the compound or the LXRα variant (e.g., McConnell
et al. (1992) Science 257:1906-1912). As used herein, a "microphysiometer" (e.g.,
Cytosensor) is an analytical instrument that measures the rate at which a cell acidifies its
environment using a light-addressable potentiometric sensor (LAPS). Changes in this
acidification rate can be used as an indicator of the interaction between a compound and
an LXRoc variant.
[00234] In yet another method, a cell-free assay is provided in which an LXRα
variant protein or biologically active portion thereof is contacted with a test compound
and the ability of the test compound to bind to the LXRα variant protein or biologically
active portion thereof is evaluated. Preferred biologically active portions of the LXRα
variant proteins to be used in assays include fragments that participate in interactions
with LXRα variant molecules, non-LXRα variant molecules (e.g., fragments with high
surface probability scores), and predicted ligand binding domains of an LXRα variant
[00235] Soluble and/or membrane-bound forms of isolated proteins (e.g., LXRα
variant proteins or biologically active portions thereof) can be used in the cell-free assays
of the invention.
[00236] Cell-free assays involve preparing a reaction mixture of the target gene
protein and the test compound under conditions and for a time sufficient to allow the two
components to interact and bind, thus forming a complex that can be removed and/or
detected using methods known in the art.
[00237] The interaction between two molecules can also be detected, e.g., using
fluorescence energy transfer (FET) (see, for example, Lakowicz et al., U.S. Patent No.
5,631,169; Stavrianopoulos, et al., U.S. Patent No. 4,868,103). A fluorophore label on
the first, 'donor' molecule is selected such that its emitted fluorescent energy will be
absorbed by a fluorescent label on a second, 'acceptor' molecule, which in turn is able to
fluoresce due to the absorbed energy. Alternately, the 'donor' protein molecule may
simply utilize the natural fluorescent energy of tryptophan residues. Labels are chosen
that emit different wavelengths of light, such that the 'acceptor' molecule label may be
differentiated from that of the 'donor'. Since the efficiency of energy transfer between the
labels is related to the distance separating the molecules, the spatial relationship
between the molecules can be assessed. In a situation in which binding occurs between
the molecules, the fluorescent emission of the 'acceptor1 molecule label in the assay

should be maximal. An FET binding event can be conveniently measured through
standard fluorometric detection means well known in the art (e.g., using a fluorimeter).
[00238] In another embodiment, determining the ability of the LXRα variant protein
to bind to a target molecule can be accomplished using real-time Biomolecular
Interaction Analysis (BIA) (e.g., Sjolander and Urbaniczky (1991) Anal. Chem. 63:2338-
2345 and Szabo et al. (1995) Curr. Opin. Struct. Biol. 5:699-705). "Surface plasmon
resonance" or "BIA" detects biospecific interactions in real time, without labeling any of
the interactants (e.g., BIAcore). Changes in the mass at the binding surface (indicative
of a binding event) result in alterations of the refractive index of light near the surface
(the optical phenomenon of surface plasmon resonance (SPR)), resulting in a detectable
signal that can be used as an indication of real-time reactions between biological
molecules.
[00239] In one embodiment, the target gene product (e.g., an LXRα variant
protein or fragment thereof) or the test substance is anchored onto a solid phase. The
target gene product/test compound complexes anchored on the solid phase can be
detected at the end of the reaction. In general, the target gene product can be anchored
onto a solid surface, and the test compound (which is not anchored) can be labeled,
either directly or indirectly, with detectable labels discussed herein.
[00240] It may be desirable to immobilize an LXRα variant, an anti-LXRα variant
antibody, or its target molecule to facilitate separation of complexed from uncomplexed
forms of one or both of the proteins, as well as to accommodate automation of the assay.
Binding of a test compound to an LXRα variant protein, or interaction of an LXRα variant
protein with a target molecule in the presence and absence of a candidate compound,
can be accomplished in any vessel suitable for containing the reactants. Examples of
such vessels include microtiter plates, test tubes, and micro-centrifuge tubes. In one
embodiment, a fusion protein can be provided that adds a domain that allows one or
both of the proteins to be bound to a matrix. For example, glutathione-S-transferase/
LXRα variant fusion proteins or glutathione-S-transferase/target fusion proteins can be
adsorbed onto glutathione Sepharose™ beads (Sigma Chemical, St. Louis, MO) or
glutathione derivatized microtiter plates, which are then combined with the test
compound or the test compound and either the non-adsorbed target protein or LXRα
variant protein, and the mixture incubated under conditions conducive to complex
formation (e.g., at physiological conditions for salt and pH). Following incubation, the
beads or microtiter plate wells are washed to remove any unbound components, the

matrix immobilized in the case of beads, complex determined either directly or indirectly,
for example, as described above. Alternatively, the complexes can be dissociated from
the matrix, and the level of LXRα variant binding or activity determined using standard
techniques.
[00241 ] Other techniques for immobilizing either a LXRα variant protein or a target
molecule on matrices include using conjugation of biotin and streptavidin. Biotinylated
LXRα variant protein or target molecules can be prepared from biotin-NHS (N-hydroxy-
succinimide) using techniques known in the art (e.g., biotinylation kit, Pierce Chemicals,
Rockford, IL), and immobilized in the wells of streptavidin-coated 96 well plates (Pierce
Chemical).
[00242] To conduct the assay, the non-immobilized component is added to the
coated surface containing the anchored component. After the reaction is complete,
unreacted components are removed (e.g., by washing) under conditions such that any
complexes formed will remain immobilized on the solid surface. The detection of
complexes anchored on the solid surface can be accomplished in a number of ways.
Where the previously non-immobilized component is pre-labeled, the detection of label
immobilized on the surface indicates that complexes were formed. Where the previously
non-immobilized component is not pre-labeled, an indirect label can be used to detect
complexes anchored on the surface; e.g., using a labeled antibody specific for the
immobilized component (the antibody, in turn, can be directly labeled or indirectly labeled
with, e.g., a labeled anti-lg antibody).
[00243] In one embodiment, this assay is performed utilizing antibodies reactive
with an LXRα variant protein or target molecules but which do not interfere with binding
of the LXRα variant protein to its target molecule. Such antibodies can be derivatized to
the wells of the plate, and unbound target or LXRα variant protein trapped in the wells by
antibody conjugation. Methods for detecting such complexes, in addition to those
described above for the GST-immobilized complexes, include immunodetection of
complexes using antibodies reactive with the LXRα variant protein or target molecule, as
well as enzyme-linked assays that rely on detecting an enzymatic activity associated with
the LXRα variant protein or target molecule.
[00244] Alternatively, cell free assays can be conducted in a liquid phase. In such
an assay, the reaction products can be separated from unreacted components by any of
a number of techniques known in the art, including but not limited to differential

centrifugation (for example, Rivas and Minton, (1993) Trends Biochem Sci 18:284-7);
chromatography (gel filtration chromatography, ion-exchange chromatography);
electrophoresis (e.g., Ausubel et al., eds. Current Protocols in Molecular Biology 1999, J.
Wiley: New York.); and immunoprecipitation (for example, Ausubel et al., eds. (1999)
Current Protocols in Molecular Biology, J. Wiley: New York). Such resins and
chromatographic techniques are known to one skilled in the art (e.g., Heegaard, (1998)
J. Mol. Recognit. 11:141-8; Hage and Tweed, (1997) J. Chromatogr. B. Biomed. Sci.
Appl. 699:499-525). Further, fluorescence energy transfer may also be conveniently
utilized, as described herein, to detect binding without further purification of the complex
from solution.
[00245] In some cases, the assay includes contacting the LXRα variant protein or
biologically active portion thereof with a known compound that binds the LXRα variant
(e.g., an LXRα, LXRα variant, or other member of the steroid receptor superfamily) to
form an assay mixture, contacting the assay mixture with a test compound, and
determining the ability of the test compound to interact with an LXRα variant protein,
wherein determining the ability of the test compound to interact with an LXRα variant
protein includes determining the ability of the test compound to preferentially bind to the
LXRα variant or biologically active portion thereof, to disrupt the interaction between the
LXRα variant and the known compound, or to modulate the activity of a target molecule,
as compared to the known compound (e.g., by monitoring dominant negative activity of
the LXRα variant).
[00246] The target gene products of the invention can, in vivo, interact with one or
more cellular or extracellular macromolecules, such as proteins. For the purposes of this
discussion, such cellular and extracellular macromolecules are referred to herein as
"binding partners." Compounds that disrupt such interactions can be useful in regulating
the activity of the target gene product. Such compounds can include, but are not limited
to molecules such as antibodies, peptides, and small molecules. The target
genes/products for use in this embodiment are generally the LXRα variant genes
identified herein. In an alternative embodiment, the invention provides methods for
determining the ability of the test compound to modulate the activity of an LXRα variant
protein through modulation of the activity of a downstream effector of a LXRα variant
target molecule. For example, the activity of the effector molecule on an appropriate

target can be determined, or the binding of the effector to an appropriate target can be
determined, as previously described.
[00247] To identify compounds that interfere with the interaction between the
target gene product and its cellular or extracellular binding partner(s), a reaction mixture
containing the target gene product and the binding partner is prepared, under conditions
and for a time sufficient, to allow the two products to form complex. In order to test an
inhibitory agent, the reaction mixture is provided in the presence and absence of the test
compound. The test compound can be initially included in the reaction mixture, or can
be added at a time subsequent to the addition of the target gene and its cellular or
extracellular binding partner. Control reaction mixtures are incubated without the test
compound or with a placebo. The formation of any complexes between the target gene
product and the cellular or extracellular binding partner is then detected. The formation .
of a complex in the control reaction, but not in the reaction mixture containing the test
compound, indicates that the compound interferes with the interaction of the target gene
product and the interactive binding partner. Additionally, complex formation within
reaction mixtures containing the test compound and normal target gene product can also
be compared to complex formation within reaction mixtures containing the test
compound and mutant target gene product. This comparison can be important in those
cases wherein it is desirable to identify compounds that disrupt interactions of mutant but
not normal target gene products.
[00248] These assays can be conducted in a heterogeneous or homogeneous
format. Heterogeneous assays involve anchoring either the target gene product or the
binding partner onto a solid phase, and detecting complexes anchored on the solid
phase at the end of the reaction. In homogeneous assays, the entire reaction is carried
out in a liquid phase. In either approach, the order of addition of reactants can be varied
to obtain different information about the compounds being tested. For example, test
compounds that interfere with the interaction between the target gene products and the
binding partners, e.g., by competition, can be identified by conducting the reaction in the
presence of the test substance. Alternatively, test compounds that disrupt preformed
complexes, e.g., compounds with higher binding constants that displace one of the
components from the complex, can be tested by adding the test compound to the
reaction mixture after complexes have been formed. The various formats are briefly
described below.

[00249] In a heterogeneous assay system, either the target gene product or the
interactive cellular or extracellular binding partner is anchored onto a solid surface (e.g.,
a microtiter plate), while the non-anchored species is labeled, either directly or indirectly.
The anchored species can be immobilized by non-covalent or covalent attachments.
Alternatively, an immobilized antibody specific for the species to be anchored can be
used to anchor the species to the solid surface.
[00250] In order to conduct the assay, the partner of the immobilized species is
exposed to the coated surface with or without the test compound. After the reaction is
complete, unreacted components are removed (e.g., by washing) and any complexes
formed will remain immobilized on the solid surface. Where the non-immobilized species
is pre-labeled, the detection of label immobilized on the surface indicates that complexes
were formed. Where the non-immobilized species is not pre-labeled, an indirect label
can be used to detect complexes anchored on the surface; e.g., using a labeled antibody
specific for the initially non-immobilized species (the antibody, in turn, can be directly
labeled or indirectly labeled with, e.g., a labeled anti-lg antibody). Depending upon the
order of addition of reaction components, test compounds that inhibit complex formation
or that disrupt preformed complexes can be detected.
[00251] Alternatively, the reaction can be conducted in a liquid phase in the
presence or absence of the test compound, the reaction products separated from
unreacted components, and complexes detected; e.g., using an immobilized antibody
specific for one of the binding components to anchor any complexes formed in solution,
and a labeled antibody specific for the other partner to detect anchored complexes.
Again, depending upon the order of addition of reactants to the liquid phase, test
compounds that inhibit complex or that disrupt preformed complexes can be identified.
[00252] In some methods, a homogeneous assay can be used. For example, a
preformed complex of the target gene product and the interactive cellular or extracellular
binding partner product is prepared in that either the target gene products or their binding
partners are labeled, but the signal generated by the label is quenched due to complex
formation (see, e.g., U.S. Patent No. 4,109,496 that utilizes this approach for
immunoassays). The addition of a test substance that competes with and displaces one
of the species from the preformed complex will result in the generation of a signal above
background. In this way, test substances that disrupt target gene product-binding
partner interaction can be identified.

[00253] In yet another aspect, the LXRα variant proteins can be used as "bait
proteins" in a two-hybrid assay or three-hybrid assay (see, e.g., U.S. Patent No.
5,283,317; Zervos et al. (1993) Cell 72:223-232; Madura et al. (1993) J. Biol. Chem.
268:12046-12054; Bartel et al. (1993) Biotechniques 14:920-924; Iwabuchi et al. (1993)
Oncogene 8:1693-1696; and Brent W094/10300), to identify other proteins, that bind to
or interact with an LXRα variant ("LXRα variant -binding proteins" or" LXRα variant -bp")
and are involved in LXRα variant activity. Such LXRα variant -bps can be activators or
inhibitors of signals (e.g., ligands) by the LXRα variant proteins or LXRα variant targets
as, for example, downstream elements of a LXRα variant -mediated signaling pathway.
Kits for performing such assays are commercially available (e.g., Stratagene, La Jolla,
CA; BD Biosciences Clontech, Palo Alto, CA).
[00254] In another embodiment, modulators of LXRα variant expression are
identified. For example, a cell or cell free mixture is contacted with a candidate
compound and the expression of an LXRα variant mRNA or protein evaluated relative to
the level of expression of the LXRα variant mRNA or protein in the absence of the
candidate compound. When expression of the LXRα variant mRNA or protein is greater
in the presence of the candidate compound than in its absence, the candidate compound
is identified as a stimulator of LXRα variant mRNA or protein expression. Alternatively,
when expression of the LXRα variant mRNA or protein is less (statistically significantly
less) in the presence of the candidate compound than in its absence, the candidate
compound is identified as an inhibitor of the LXRα variant mRNA or protein expression.
The level of the LXRα variant mRNA or protein expression can be determined by
methods described herein for detecting the LXRα variant mRNA or protein.
[00255] In another aspect, the invention pertains to a combination of two or more
of the assays described herein. For example, a modulating agent can be identified using
a cell-based or a cell free assay, and the ability of the agent to modulate the activity of an
LXRα variant protein can be confirmed in vivo, e.g., in an animal such as an animal
model for hypercholesterolemia, or other disorder related to fatty acid metabolism.
[00256] This invention further pertains to novel agents identified by the above-
described screening assays. Accordingly, it is within the scope of this invention to
further use an agent identified as described herein (e.g., an LXRα variant modulating
agent, an antisense LXRα variant nucleic acid molecule, an LXRα variant -specific
antibody, or an LXRα variant -binding partner) in an appropriate animal model to

determine the efficacy, toxicity, side effects, or mechanism of action, of treatment with
such an agent. Furthermore, novel agents identified by the above-described screening
assays can be used for treatments as described herein.
Transgenic Animals
[00257] The invention also relates to non-human transgenic animals. Such
animals are useful for studying the function and/or activity of an LXRα variant protein and
for identifying and/or evaluating modulators of LXRα variant expression or activity. As
used herein, a "transgenic animal" is a non-human animal, such as a mammal, e.g., a
rodent such as a rat or mouse, in which one or more of the cells of the animal includes a
transgene. Other examples of transgenic animals include non-human primates, sheep,
dogs, cows, goats, chickens, amphibians, and the like. A transgene is exogenous DNA
or a rearrangement, e.g., a deletion of endogenous chromosomal DNA, which generally
is integrated into or occurs in the genome of the cells of a transgenic animal. A
transgene can direct the expression of an encoded gene product in one or more cell
types or tissues of the transgenic animal, other transgenes, e.g., a knockout, reduce
expression. Thus, a transgenic animal can be one in which an endogenous LXRα
variant gene has been altered by, e.g., by homologous recombination between the
endogenous gene and an exogenous DNA molecule introduced into a cell of the animal,
e.g., an embryonic cell of the animal, prior to development of the animal. In some cases
the ortholog of the LXRα variant is identified in the animal and ortholog sequence is used
to generate the transgenic animal. When homology is sufficient between the known
(e.g., human) and LXRα variant gene of interest, the human sequence can be used.
[00258] Intronic sequences and polyadenylation signals can also be included in
the transgene to increase the efficiency of expression of the transgene. A tissue-specific
regulatory sequence(s) can be operably linked to a transgene of the invention to direct
expression of an LXRα variant protein to particular cells. A transgenic founder animal
can be identified based upon the presence of an LXRα variant transgene in its genome
and/or expression of the LXRα variant mRNA in tissues or cells of the animals.
Transgenic animals can also be identified by other characteristics associated with the
transgene. For example, a transgenic animal expressing an LXRα-64 transgene will
have a decreased amount of SREBP-1C expression, which is particularly notable in the
presence of an LXRα agonist compared to a control animal. A transgenic founder

animal can then be used to breed additional animals carrying the transgehe. Moreover,
transgenic animals carrying a transgene encoding an LXRoc variant protein can further be
bred to other transgenic animals carrying other transgenes.
[00259] LXRα variant proteins or polypeptides can be expressed in transgenic
animals, e.g., a nucleic acid encoding the protein or polypeptide can be introduced into
the genome of an animal. In general, the nucleic acid is placed under the control of a
tissue specific promoter, e.g., a milk or egg specific promoter, and recovered from the
milk or eggs produced by the animal. Suitable animals for this application include mice,
pigs, cows, goats, and sheep.
[00260] The invention also includes a population of cells from a transgenic animal.
Methods of isolating and propagating such cells are known in the art and include the
development and propagation of primary, secondary, and immortalized cells.
EXAMPLES
[00261] The present invention is further defined in the following Examples, in
which all parts and percentages are by weight and degrees are Celsius, unless
otherwise stated. It should be understood that these Examples, while indicating
examples of embodiments of the invention, are given by way of illustration only. From
the above discussion and these Examples, one skilled in the art can ascertain the
essential characteristics of this invention, and without departing from the spirit and scope
thereof, can make various changes and modifications of the invention to adapt it to
various usages and conditions. The Examples are not to be construed as limiting the
scope or content of the invention in any way.
EXAMPLE 1
Cloning of Human LXR Variants
[00262] Total RNA was isolated from THP-1 cells (human monocyte-macrophage
cell line using a QIAGEN Kit (QIAGEN, Valencia, CA). The first-strand cDNA was
synthesized from 0.1 ug of total THP-1 RNA in a 20 uL reaction mixture containing 4 uL
of 5XRT reaction buffer, 10 units of Rnasin, 200 u.M dNTP, 20 pM random primer, and
20 units of reverse transcriptase. The mixture was incubated at 42° C for 1 hour and
then at 53° C for 30 minutes. The unhybridized RNA was then digested with 10 units of

RNase H at 37° C for 10 minutes. Two uL of the reverse transcriptase products were
subjected to PCR amplification using human LXRα-specific primers. The primer
sequences were LXRα -For: 5'-CGGTCGACATGTCCTTGTGGCTGGGG (SEQ ID
NO:9); and LXRα-Rev: 5'-CAGCGGCCGCTTCGTGCACATCCCAGATCTC (SEQ ID
NO:10) (restriction sites are underlined). Thirty-five cycles of amplification were
performed in a thermocycler at 94° C (30 seconds), 58° C (30 seconds), and 72° C (2
minutes). The RT-PGR products were analyzed on a 1.2% agarose gel. The same
amount of total RNA was used as a template in the PCR to verify that the band was
amplified from cDNA. The RT-PCR products were sub-cloned into the Sal I/Nit I sites of
pCMV expression vector for sequencing. The result of sequencing the subclones was
the identification of a number of novel sequences, including those termed herein LXRα-
64, LXRα-42e+, AND LXRα-42e'.
EXAMPLE 2
Sequencing and Preliminary Analysis of the Clone
[00263] Using the LXRα-For and LXRα-Rev primers of Example 1 {supra), three
alternative variants of human LXRα were identified and cloned from human
monocyte/macrophage THP-1 cells. The variants were LXRα-64, which was found to be
64 amino acids longer than the native (wild-type) LXRα; LXRα-42e+, which has 42 amino
acids different from native LXRα; and LXRα-42e", which has 42 amino acids different
from native LXRα and the sequence corresponding to exon 6 of native LXRα is missing.
The comparison of nucleotide sequences and predicted amino acid sequences of the
new LXRα variants with wild-type human LXRα are shown in Figs. 1B, 2B, and 3B.
[00264] Fig. 1A illustrates the novel nucleotide sequence that is present in LXRα-
64 that is not present in wild type LXRα (nucleotides 1121-1154). Fig. 1B illustrates the
novel amino acid sequence that is present in LXRα-64 that is not present in wild type
LXRα (amino acids 368-409).
[00265] Fig. 2A illustrates the novel nucleotide sequence that is present in LXRα-
42e+. The missing sequence in LXRα-42e+ that is present in wild type LXRα
(nucleotides 1121-1154) introduces a frame shift. This results in a novel amino acid
sequence in LXRα-42e+ (amino acids 368-409 of LXRα-42e+). LXRα-42e+ lacks the
amino acid sequence corresponding to amino acids 368-447 of wild type LXRα-42e+.

[00266] Fig. 3A depicts the complete sequence for LXRoc-42e- from nucleotides
651-1220. This figure does not depict the entire sequence of wild type LXRαfrom the
corresponding region (nucleotides 651-1166). The sequence corresponding to
nucleotides 708-887 of wild type LXRα are not present in LXRα-42e-. The sequence
corresponding to nucleotides 1101-1134 of LXR-42e- is not present in wild type LXRα.
Fig. 3B shows sequences that are present in wild type LXRα and not in LXRα42e-
(amino acids 237-296 and 368-447 of wild type LXRα) and sequences that are present
only in LXRα-42e- (amino acids 308-349 of LXRα-42e-).
[00267] The entire cDNA coding region and the predicted amino acid sequence of
the new variants are shown in SEQ ID NO:3 (nucleotide sequence coding for LXRα-64),
SEQ ID NO:4 (deduced amino acid sequence of LXRα-64), SEQ ID NO:5 (nucleotide
sequence coding for LXRα-42e+ cDNA), SEQ ID NO:6 (deduced amino acid sequence
of LXRα-42e+), SEQ ID NO:7 (nucleotide sequence coding for LXRα-42e"), SEQ ID
NO:8 (deduced amino acid sequence of LXRα-42e"), SEQ iD NO:16 (unique nucleotide
sequence of LXRα-64 that connects exons 6 and 7 of wild type LXRα, derived from
intron 6, creating a larger exon 6), SEQ ID NO:17 (unique amino acid sequence in
LXRα-64 and encoded by SEQ ID NO:16), SEQ ID NO:18 (the novel portion of exon 8 in
LXRα-42e mRNAs that is not present in exon 8 of wild-type LXRα, and SEQ ID NO:19
(deduced amino acid sequence encoded by the additional sequence identified in LXRα-
42 cDNAs).
EXAMPLE 3
Gene Characterization
[00268] The genomic organization of the novel variants of the present invention,
LXRα-64, LXRα-42+ and LXRα-42", was determined. Transcription start sites, genomic
structure, alternative splicing, and functional domains of the LXRα-64, LXRα-42+ and
LXRα-42" and their comparison with wild type LXRα are described in Figs. 4, 5, and 6
respectively.
[00269] Fig. 4 diagrams the structure of LXRα-64 mRNA, showing that novel
sequence is incorporated into sequence corresponding to exon 6 of wild type LXRα.
Therefore, a probe having the novel sequence is useful for, e.g., identifying the
expression of an LXRα-64 or identifying LXRα-64 variants. The amino acid sequence

encoded by the novel sequence can be used as an antigen to generate an antibody that
specifically binds to LXRoc-64 variants. It is a characteristic of LXRα-64 variants that
their mRNAs contain the novel sequence nucleic acid sequence and encode the novel
amino acid sequence. Such variants may contain conservative substitutions.
[00270] Fig. 5 diagrams the structure of LXRα-42e+ mRNA, showing that novel
sequence is incorporated into sequence corresponding to exon 8 of wild type LXRα, the
sequence introducing a stop signal into the sequence preceding exon 9. The new
LXRα-42e+ sequence also lacks exon 10 of wild type LXRα. A probe having the novel
sequence is useful for, e.g., identifying the expression of an LXRoc-42e+ or identifying
LXRoc-42e+ variants. It is a characteristic of LXRα-42e+ variants that their mRNAs
contain the novel nucleic acid sequence and encode the novel amino acid sequence.
Such variants may contain conservative substitutions. Certain LXRα-42e+ variants lack
exon 10. In some cases an LXRα-42e+ variant contains both the novel sequence and
lacks exon 10.
[00271] Fig. 6 diagrams the structure of LXRα-42e" mRNA, showing that exon 6 of
wild type LXRα is absent in LXRα-42e". (Some reports of wild type LXRα designate
exon 1 as exon 1A and exon 2 as exon 1B. Under this terminology, exon 5 of the wild
type LXRα corresponds to the missing exon 6 sequence.) A probe that includes the
contiguous exon 5 and exon 7 sequence of LXRα-42e~ is therefore useful, e.g., for
specifically detecting expression of this sequence or for identifying novel variants of
LXRα-42e". Accordingly, a characteristic of an LXRα-42e" variant is the lack of wild type
exon 6. An amino acid sequence that is encoded by the sequence bridging exons 5 and
7 is also useful for generating an antibody that specifically binds to an LXRα-42eEXAMPLE 4
Tissue Distribution
[00272] Tissue distribution studies were performed using real-time PCR and
Multiple Tissue cDNA panels (MTC, human cDNA) from BD Biosciences Clontech (Palo
Alto, CA). Real-time quantitative PCR assays were performed on the panels using an
Applied Biosystems 7700 sequence detector (Foster City, CA). Each amplification
mixture (50 uL) contained 50 ng of cDNA, 400 nM forward primer (SEQ ID NO:11), 400
nM reverse primer (SEQ ID NO:12), 200 nM dual-labeled fluorogenic probe (SEQ ID
NO:13) (Applied Biosystems), 5.5 mM MgCI2, and 1.25 units Gold Taq (Applied

Biosystems). The primers amplify a portion of the LXRα sequences that is about 80
nucleotides in length. The PCR thermocycling parameters were 95° C for 10 minutes,
and 40 cycles at 95° C for 15 seconds, and 60° C for 1 minute. Together with the
samples and no-template controls, a serially diluted cDNA standard was analyzed in
parallel. All samples were analyzed for human glyceraldehyde-3-phosphate
dehydrogenase (GAPDH) expression in parallel in the same run using probe and
primers from predeveloped assays for GAPDH (Applied Biosystems). All of the target
gene expression was normalized to the expression of GAPDH. Quantitative analysis
was performed using the threshold procedure, following the manufacturer's protocol
(Perkin-Elmer), and relative amounts were calculated from the standard curve.
[00273] The primers and probe used to detect LXRα variant LXRα-64 in these
studies were as follows: L64-For (5'-TGGGAAGCAGGGATGAGG-3'; SEQ ID NO:11),
L64-Rev (5'-GAGGGCTGGTCTTGGAGCA-3'; SEQ ID NO:12), and L64TaqMan probe
(FAM-TCGGCCTCCCTGGAAGAGGCC-TAMRA; SEQ ID NO:13). The L64 primers
and probe are localized to the 64 nucleotides that are found in the LXRα-64 cDNA.
[00274] LXRα-64 mRNA was found to be most abundantly expressed in liver (Fig.
7). Transcripts were also detected at a relatively high level in small intestine, placenta,
pancreas, ovary, and colon. Very little expression was observed in the other tester
tissues. The primers and probe used to detect LXRα variant LXRα-42 in these studies
were as follows: L42-For (5'-GGTGGAGGCATTTGCTGTGT-3'; SEQ ID NO:21), L42-
Rev (5'-CCCAAATTGCAACCAAAATATAGA-3*; SEQ ID NO:22) and L42 probe (FAM-
TTTAGGATGAGAGAGCTTGGCTGGAGCAT-TAMRA; SEQ ID NO:23). FAM/TAMRA
fluorogenic probes are available from BioSearch Technologies (Novato, CA).
[00275] The expression of LXRα-42 had different pattern compared to LXRα-64.
While the most abundant expression was observed in liver, the LXRα-42 sequences
were detected only at low levels or were absent in the other tissues tested.
[00276] Wild type LXRα as well as LXRα variants are highly expressed in liver.
Next to liver, wild type LXRα is present in the greatest abundance in pancreas followed
by testis, small intestine, and spleen, which share similar levels of mRNA. Prostate,
thymus, kidney, ovary, placenta, lung, and colon express less than testis, while
leukocyte, heart, brain, and skelet al muscle contain negligible amounts of wild type
LXRα mRNA. LXRα-64 is also expressed at the highest level in liver followed by small
intestine. Placenta, pancreas, ovary, colon, and lung express less LXRα-64 than small

intestine. Expression was observed to be even lower in kidney and leukocyte, while
heart, brain, skelet al muscle, spleen, thymus, prostate, and testis contained negligible
amounts of expression. LXRα-42 expression (LXRα-42e" plus LXRα-42e+) in lung was
lower than in liver. The remaining tissues (discussed supra) had significantly lower
levels of expression compared to liver.
EXAMPLE 5
Upregulation of LXRL64 by LXR agonists in dTHP-1 cells
[00277] Experiments were performed to determine whether agonists of wild type
LXRoc could also regulate the expression of LXRα variants. In these experiments,- THP-1
cells were obtained from the American Type Culture Collection (ATCC) and cultured in
RPMI medium containing 10% fet al bovine serum (FBS). For gene expression analysis
in differentiated THP-1 cells, the THP1 cells were incubated in RPMI medium
supplemented with 10% lipoprotein-deficient serum (LPDS) (Intracel Corp, Rockville,
MD) and treated with 150 nM phorbol ester for 3 days followed by treatment with LXR,
RXR, or Peroxisome Proliferator-activated Receptor y (PPARγ) agonist compounds,
specifically with vehicle only (control), 10 uM T0901317, 10 uM GW 3965, 10 uM
Ciglitazone, or 1 uM 9RA. The primers and TaqMan probe for the real-time RT-PCR
was described as in Example 4. The data showed that expression of LXRα-64 and
LXRα-42 mRNAs was increased in THP-1 cells incubated with either of the two synthetic
LXR agonists T0901317 ([N-(2,2,2,-trifluoro-ethyl)-N-[4-(2,2,2,-trifluoro-1-hydroxy-1-
trifluoromethyl-ethyl)-phenyl]-benzenesulfonamide]) (Repa et al., Science 2000
289(5484): 1524-9, and Schultz et al., Genes Dev. 2000 14(22):2831-8), GW3965 [3-(3-
(2-chloro-3-trifluoromethylbenzyl-2,2-diphenylethylamino)propoxy)phenylaceticacid]
(Collins et al., J. Med. Chem., 2002 45:1963-1966 and Laffitte et al., Mol. Cell. Biol.
2001, 21: 7558-7568), PPARγ ligand (10 uM of citglitazone), and RXR ligand (9-cis
retinoic acid) (Figs. 8A and 8B).
[00278] These data demonstrate that expression of LXRα variants can be induced
using known LXRα agonists.
EXAMPLE 6
Functional Characterization of LXRα variants
[00279] Human LXRα promoter (SEQ ID NO:14) was amplified by PCR using
information from the published LXRα genomic structure and sequence (GenBank

accession no. AC090589. A fragment spanning from -2660 to -2363 (relative to the
transcription start site from exon 1) of LXRα promoter which contains the LXR response
element (5'-TGACCAgcagTAACCT-3', SEQ ID NO:20) (Laffitte et al. 2001, Mol. Cell.
Biol. 21, 7558-7568 and Whitney et al., 2001, J. Biol. Chem. 276, 43509-43515) of LXRα
was subcloned into pGL3 basic plasmid to create pGL-3-LXRoc-Luc. The GenBank
accession number of the LXRα "native" sequence used as a reference for the
experiments and analysis disclosed herein is Genbank accession number for human
LXRα is BC008819. Coding regions of human LXRα, and RXRa (GenBank accession
number BC007925) were amplified by RT-PCR according to the sequences in GenBank
and subcloned into pCMV/myc/nuc expression vectors (Invitrogen, Carlsbad, CA). The
new LXRα-L64 coding region was subcloned into pCMV/myc/nuc expression vectors.
[00280] HEK 293 cells were grown in Dulbecco's modified Eagle's medium
(DMEM) containing 10% FBS. Transfections were performed in triplicate in 24 well
plates using Lipofectamine 2000 (Invitrogen). Each well was transfected with 400 ng of
reporter plasmid, 100 ng of receptor expression vector, and 200 ng of pCMV-Bgal
reference plasmid containing a bacterial (3-galactosidase gene. Additions to each well
were adjusted to contain constant amounts of DNA and of pCMV (Invitrogen, Carlsbad,
CA) expression vector. After six to eight hours following transfection, the cells were
washed once with phosphate-buffered saline (PBS), and then incubated with fresh
medium containing 10% lipoprotein-deficient serum (LPDS) (Intracel Corp, Rockville,
MD) and an LXR agonist, RXR agonist, or vehicle control for 24 hours. The cells were
harvested, analyzed, and the extracts were assayed for luciferase and B-galactosidase
activity in a microplate luminometer/photometer reader (Lucy-1; Anthos, Salzburg,
Austria). Luciferase activity was normalized to B-galactosidase activity.
[00281] In more detail, HEK 293 cells were contransfected with either control
pGL3-basic vector (Promega Madison, WI 53711) or pGL3- LXRα -Luc (part of LXRα
promoter containing the LXRE sequence of LXRα promoter (TGACCAgcagTAACCT;'
SEQ ID NO:20) was subcloned into Kpn l/Xho I sites of pGL3-basic vector) reporters
with pCMV-h LXRα/pCMV-hRXRa, pCMV- LXRα-64/pCMV-hRXRa, pCMV- LXRα-
42e7pCMV-hRXRa, pCMV- LXRα-42e7pCMV-hRXRa respectively. Following
transfection, cells were incubated for 24 hours in DMEM supplemented with 10%
lipoprotein-deficient serum (LPDS) and 10 u.M T0901317 or vehicle control then
luciferase activity assayed and normalized.

[00282] As shown in Fig. 9, when the new LXRoc variants were co-transfected with
the reporter gene, the LXR ligand-dependent activation was sharply decreased as
compared with the co-transfected native LXRoc. Furthermore, as shown in Fig. 10, when
the variants and LXRα were simultaneously co-transfected with the reporter gene, the
activation of exogenous LXRα was inhibited as compared with LXRα co-transfected
along. These data indicated that the newly cloned LXRα variants can function as
dominant negative regulators of native LXRα expression.
EXAMPLE 7
Regulation of LXR target genes by LXR variant
[00283] An important feature of LXRα is its involvement in multiple physiologic effects,
some of which are advantageous to an organism and some of which are, at least in certain
cases, deleterious to the organism. Thus, the discovery described herein of new LXRα varia
provides targets to permit the differentia! regulation of different aspects of LXRα activity in a (
To determine the function of the variants, the expression of LXR target genes in the presence
an expressed LXRα variant was examined.
[00284] In these experiments, coding regions of human LXRα, RXRa, and the LXRα
variant (LXRα-64) were amplified by RT-PCR. The PCR products were subcloned into
pCMV/myc/nuc expression vectors (Invitrogen, Carlsbad, CA) and used in the experiments
described infra.
[00285] Expression experiments were conducted in HEK 293 cells that were propagati
in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS. The cultured cells we
transfected with either the expression vector containing a sequence encoding LXRα (wild typ
or an expression vector encoding LXRα-64. All samples were co-transfected with an
expression vector encoding an RXRa sequence. Transfections were performed in triplicate i
24 well plates using the Lipofectamine 2000 (Invitrogen, Carlsbad, CA). Each well was
transfected with 200 ng of the LXRα expression vector (LXRα), the LXRα-64 expression vea
(L64), or control plasmid (pCMV) along with 200 ng of human RXRa expression vector (RXR
Additions to each well were adjusted to contain constant amounts of DNA and of pCMV
expression vector. Six to eight hours following transfection, the cells were washed once with
phosphate-buffered saline (PBS), then incubated with fresh medium containing 10% lipoproti
deficient serum (LPDS) (Intracel Corp, Rockville, MD) and a synthetic LXR agonist (TO9013"
and/or RXR agonist (9-cis-retinoic acid, 9RA), or vehicle only (control) for 48 hours. The cell

were then harvested and total RNA was isolated from the cells using a QIAGEN kit. The levi
of gene expression were determined with Real-time quantitative PCR assays using an Applie
Biosystems 7700 sequence detector.
[00286] When a sequence encoding the new variant, LXRoc-64, was cotransfected witl
human RXRoc-encoding sequence and expressed in HEK 293 cells, basal, LXR ligand-
dependent, and LXR+RXR ligand-dependent induction of SREBP-d (an LXR target gene)
expression was sharply decreased compared to expression of SREBP-c1 in cells transfectec
with either wild type LXRoc with RXRa or empty expression vector with RXR a (Fig. 11). The
basal expression of another LXR target gene, ABCA1 was not affected by the introduction o
the variant L64 with RXRa into the cells. However, LXR as well as LXR + RXR-ligand
dependent induction of ABCA1 expression was less in cells expressing LXRα-64 and RXRo
compared to expression in cells transfected with native LXRα and RXRa or empty
expression vector with RXRa. (Fig. 12).
[00287] These data demonstrate that the LXRα variants can differentially regulate the
expression of LXR target genes in HEK 293 cells, serving as dominant negative modulators ■
LXRα-induced gene expression. Thus, regulating expression or activity of an LXRα variant
provides a method of differentially regulating LXRα-associated effects in cells.
[00288] These data also demonstrate that over expressing an LXRα variant can
inhibit SREBP-C1 expression. Also, induction of expression of SREBP-1C by an LXR
' agonist is significantly decreased in a cell expressing an LXRα variant (e.g., LXRα-64.
Therefore, increasing the expression or activity of an LXRα variant (e.g., LXRα-64) is
useful for treating disorders associated with the expression of SREBP-1C. For example,
disrupting the activity of an LXRα, e.g., by over expressing an LXRα-64 or increasing the
activity of an LXRα-64 that is expressed in a cell (e.g., by administering a compound that
differentially binds to LXRα-64 compared to wild type LXRα) can provide a method of
inhibiting the insulin induction of SREBP-1C, and therefore provides a method of
inhibiting undesirable induction of fatty acid synthesis by insulin. In another example,
over expressing an LXRα variant (e.g., LXRα-64) or selectively activating an LXRα
variant (for example, with a compound that differentially binds to the LXRα-variant) can
result in inhibition of SREBP-1C, and therefore provides a method of treating
hypertriglyceridemia, which is a condition that is a strong predictor of heart disease. In
another example, lowered SREBP-1C expression (by increased expression or activity of
an LXRα variant such as LXRα-64) can result in lower expression of VLDL-TGs (very

low density lipoprotein triglycerides), a desirable effect in certain disorders such as
diabetes and certain types of hyperlipoproteinemia.
[00289] Wild type LXR expression in the presence of an LXR agonist has the
effect of upregulating ABCA1, which is involved in reverse cholesterol transport.
Expression of an LXRoc variant (e.g., LXRoc-64) has little apparent effect on cellular
processes. Therefore, overexpression of an LXRα variant can be beneficial in that it
decreases expression of a particular LXRα target gene (e.g., SREBP-1C) but does not
affect another LXRα target gene whose expression may be desirable (e.g., ABCA1).
[00290] Nuclear receptors that heterodimerize with RXR and activation of these
heterodimers results in increased expression of specific genes. In the case of
undesirable expression of one or more of these genes (e.g., LXR-mediated upregulation
of SREBPIc), then overexpression of an LXRα-64 can be beneficial to a subject if
expression of the LXRα variant binds to the RXR, thereby decreasing the availability of
the RXR for heterodimerization and therefore reducing induction undesirable gene
expression.
Sequences
SEQ ID NO;l
cDNA of the entire coding region, of wild type LXRα
1 atgtccttgt ggctgggggc ccctgtgcct gacattcctc ctgactctgc
51 ggtggagctg tggaagccag gcgcacagga tgcaagcagc caggcccagg
101 gaggcagcag ctgcatcctc agagaggaag ccaggatgcc ccactctgct
151 gggggtactg caggggtggg gctggaggct gcagagccca cagccctgct
201 caccagggca gagccccctt cagaacccac agagatccgt ccacaaaagc
251 ggaaaaaggg gccagccccc aaaatgctgg ggaacgagct atgcagcgtg
301 tgtggggaca aggcctcggg cttccactac aatgttctga gctgcgaggg
351 ctgcaaggga ttcttccgcc gcagcgtcat caagggagcg cactacatct
401 gccacagtgg cggccactgc cccatggaca cctacatgcg tcgcaagtgc
451 caggagtgtc ggcttcgcaa atgccgtcag gctggcatgc gggaggagtg
501 tgtcctgtca gaagaacaga tccgcctgaa gaaactgaag cggcaagagg

551 aggaacaggc tcatgccaca tccttgcccc ccaggcgttc ctcacccccc
601 caaatcctgc cccagctcag cccggaacaa ctgggcatga tcgagaagct
651 cgtcgctgcc cagcaacagt gtaaccggcg ctccttttct gaccggcttc
701 gagtcacgcc ttggcccatg gcaccagatc cccatagccg ggaggcccgt
751 cagcagcgct ttgcccactt cactgagctg gccatcgtct ctgtgcagga
801 gatagttgac tttgctaaac agctacccgg cttcctgcag ctcagccggg
851 aggaccagat tgccctgctg aagacctctg cgatcgaggt gatgcttctg
901 gagacatctc ggaggtacaa ccctgggagt gagagtatca ccttcctcaa
951 ggatttcagt tataaccggg aagactttgc caaagcaggg ctgcaagtgg
1001 aattcatcaa ccccatcttc gagttctcca gggccatgaa tgagctgcaa
1051 ctcaatgatg ccgagtttgc cttgctcatt gctatcagca tcttctctgc
1101 agaccggccc aacgtgcagg accagctcca ggtggagagg ctgcagcaca
1151 catatgtgga agccctgcat gcctacgtct ccatccacca tccccatgac
1201 cgactgatgt tcccacggat gctaatgaaa ctggtgagcc tccggaccct
1251 gagcagcgtc cactcagagc aagtgtttgc actgcgtctg caggacaaaa
1301 agctcccacc gctgctctct gagatctggg atgtgcacga atga

SEQ ID NO:3

The cDNA sequence that codes for LXR-64
1 atgtccttgt ggctgggggc ccctgtgcct gacattcctc ctgactctgc
51 ggtggagctg tggaagccag gcgcacagga tgcaagcagc caggcccagg
101 gaggcagcag ctgcatcctc agagaggaag ccaggatgcc ccactctgct
151 gggggtactg caggggtggg gctggaggct gcagagccca cagccctgct
2 01 caccagggca gagccccctt cagaacccac agagatccgt ccacaaaagc
251 ggaaaaaggg gccagccccc aaaatgctgg ggaacgagct atgcagcgtg
3 01 tgtggggaca aggcctcggg cttccactac aatgttctga gctgcgaggg
351 ctgcaaggga ttcttccgcc gcagcgtcat caagggagcg cactacatct
401 gccacagtgg cggccactgc cccatggaca cctacatgcg tcgcaagtgc
451 caggagtgtc ggcttcgcaa atgccgtcag gctggcatgc gggaggagtg
501 tgtcctgtca gaagaacaga tccgcctgaa gaaactgaag cggcaagagg
551 aggaacaggc tcatgccaca tccttgcccc ccaggcgttc ctcacccccc
601 caaatcctgc cccagctcag cccggaacaa ctgggcatga tcgagaagct
651 cgtcgctgcc cagcaacagt gtaaccggcg ctccttttct gaccggcttc
701 gagtcacgcc ttggcccatg gcaccagatc cccatagccg ggaggcccgt
751 cagcagcgct ttgcccactt cactgagctg gccatcgtct ctgtgcagga
801 gatagttgac tttgctaaac agctacccgg cttcctgcag ctcagccggg
851 aggaccagat tgccctgctg aagacctctg cgatcgaggt ggctggagaa
901 gggcaaggga tgaagggaga agcagagtgg gattatctgt gggaggggcc
951 tccagacatc gagctgggag agccaaatct gctgggaagc agggatgagg
1001 agaatcggcc tccctggaag aggccatgct ccaagaccag ccctcctagt
1051 ccccgtttga ggtttgctgc ttgtgtgcag gtgatgcttc tggagacatc
1101 tcggaggtac aaccctggga gtgagagtat caccttcctc aaggatttca
1151 gttataaccg ggaagacttt gccaaagcag ggctgcaagt ggaattcatc
1201 aaccccatct tcgagttctc cagggccatg aatgagctgc aactcaatga
1251 tgccgagttt gccttgctca ttgctatcag catcttctct gcagaccggc
13 01 ccaacgtgca ggaccagctc caggtggaga ggctgcagca cacatatgtg

1351 gaagccctgc atgcctacgt ctccatccac catccccatg accgactgat
1401 gttcccacgg atgctaatga aactggtgag cctccggacc ctgagcagcg
1451 tccactcaga gcaagtgttt gcactgcgtc tgcaggacaa aaagctccca
1501 ccgctgctct ctgagatctg ggatgtgcac gaatga

251 ggacatctgg gttctgatcc cagcccagcc actaactgtg tggtcttgga
SEQ ID NO:15
The nucleotide sequence of the LXR response element (LXRE)
5'-AGGTCAnnnnAGGTCA-3'
SEQ ID NO:16
The unique nucleotide sequence of the LXRα-64 variant that forms a new,
larger exon G and connects exons 6 and 7 of wild type LXRα
GCTGGAGAAG GGCAAGGGAT GAAGGGAGAA GCAGAGTGGG ATTATCTGTG GGAGGGGCCT
CCAGACATCG AGCTGGGAGA GCCAAATCTG CTGGGAAGCA GGGATGAGGA GAATCGGCCT
CCCTGGAAGA GGCCATGCTC CAAGACCAGC CCTCCTAGTC CCCGTTTGAG GTTTGCTGCT
TGTGTGCAGG TG
SEQ ID NO:17
The deduced amino acid sequence encoded by SEQ ID NO: 16
VAGEGQGMKGEAEWDYLWEGPPDIELGEPNLLGS RDEENRPPWKRPCSKTSPPSPRLRFAACVQ
SEQ ID NO:18
The unique nucleotide sequence of LXRot-42e that forms a new exon 8 that
includes exon 8 of wild type LXRα and creates a longer exon 8 LXRα-42
variant.
GTGTGGAGGA GGGGCAATGG GAAACAGCAA GAGACTTACA CCAAGGAGGG CTGCAGGTCC
CACAGGAATC GGTGGGGGGA GGGGGGTGGT GGCTTGGGAG GGTGGAGGCA TTTGCTGTGT
TATTTTAGGA TGAGAGAGCT TGGCTGGAGC ATGTCTCTAT ATTTTGGTTG CAATTTGGGG
TATGGAACTG GACCCTGGCC AGACCTGCTC CTCAACTCTC TTGGTGACCT ATAG
SEQ ID NO:19
The deduced amino acid sequence encoded by SEQ ID NO: 18
GVEEGQWETARDLHQGGLQVPQESVGGGGWWLGRVEAFAVLF
SEQ ID NO:20


Other Embodiments
[00291] It is to be understood that while the invention has been described in conjunctk
with the detailed description thereof, the foregoing description is intended to illustrate and no'
limit the scope of the invention, which is defined by the scope of the appended claims. Othei
aspects, advantages, and modifications are within the scope of the following claims.

WE CLAIM:
1. An isolated nucleic acid molecule encoding a human liver X receptor alpha (LXRa) variant
polypeptide selected from the group consisting of:
(a) an isolated nucleic acid molecule encoding SEQ ID NO: 6, 8, 17, or 19;
(b) an isolated nucleic acid molecule encoding an amino acid sequence having at least
90% identity with the SEQ ID NO:, 6, 8, 17, or 19;
(c) an isolated nucleic acid molecule that hybridizes with the isolated nucleic acid
molecule of (a) or (b) under hybridization conditions of 6X SSC (1M NaCI), 50 %
formamide, 1 % SDS at 42 °C, and a wash in 1X SSC at 42°C, and a wash at 68°C, in
0.2XSSC, and 0.1% SDS; and
(d) an isolated nucleic acid molecule that is complementary to (a), (b), or (c).

2. The isolated nucleic acid molecule of claim 1 consisting of SEQ ID NO, 5, 7, 16, or 18.
3. The isolated nucleic acid molecule of claim 1, wherein the isolated nucleic acid molecule is a
DNA molecule.
4. The isolated nucleic acid molecule of claim 1, wherein the isolated nucleic acid molecule is an
RNA molecule.
5. The isolated nucleic acid molecule of claim 1, wherein the isolated nucleic acid molecule
comprises SEQ ID NO:, 5, 7, 16, or 18.
6. The nucleic acid molecule of claim 1, wherein the nucleic acid molecule encodes a
polypeptide that has LXR-responsive pathway activity.
7. The nucleic acid molecule of claim 1, wherein the polypeptide encoded by the isolated nucleic
acid molecule can form a dimer with a wild-type LXR.
8. The nucleic acid molecule of claim 1, wherein the polypeptide encoded by the isolated nucleic
acid molecule can form a heterodimer with a retinoid X receptor (RXR).

9. The nucleic acid molecule of claim 8, wherein the RXR is an RXR, RXR, or RXRy.
10. A polypeptide encoded by the isolated nucleic acid molecule of claim 1.
11. The polypeptide of claim 10, wherein the polypeptide can form a dimer with a wild-type LXRα.
12. The polypeptide of claim 10, wherein the polypeptide can form a heterodimer with an RXR.
13. The polypeptide of claim 12, wherein formation of the heterodimer can inhibit formation of a
heterodimer between the RXR and a nuclear receptor with which the RXR naturally heterodimerizes.
14. The polypeptide of claim 12, wherein formation of the heterodimer can inhibit formation of a
homodimer of the RXR.
15. The polypeptide of claim 12, wherein, RXR is an RXR, RXR or RXRγ.
16. The polypeptide of claim 10, wherein the polypeptide can exhibit LXRα dominant negative
activity.
17. The polypeptide of claim 12, wherein the LXRα variant is a fragment of an LXRα-64, an
LXRα-42+, or an LXRα-42".
18. The polypeptide of claim 17, wherein the fragment of an LXRα variant can exhibit at least one
function of an LXRα variant.
19. A construct comprising an isolated nucleic acid molecule of claim 1.
20. The construct of claim 19, wherein the isolated nucleic acid molecule is operatively linked to a
regulatory sequence.

21. The construct of claim 19, wherein the construct is a plasmid.
22. The construct of claim 19, wherein the construct comprises pCMV/myc or pcDNA 3.1, or is a
derivative thereof.
23. A host cell comprising an isolated nucleic acid molecule of claim 1 or a descendent of the
cell.
24. A host cell comprising the construct of claim 19.
25. The host cell of claim 23, wherein the host cell is a prokaryotic cell.
26. The host cell of claim 23, wherein the host cell is an E. coli.
27. The host cell of claim 23, wherein the host cell is a mammalian cell.
28. The host cell of claim 23, wherein the host cell is a human cell.
29. The host cell of claim 23, wherein the host cell is a human embryonic cell.
30. The host cell of claim 23, wherein the host cell is selected from the group consisting of a
human hepatoma cell (HepG2), a Chinese hamster ovary cell (CHO), a monkey COS-1 cell, and a
human embryonic kidney cell (HEK 293).
31. The host cell of claim 23, wherein, the host cell is selected from the group consisting of a
Saccharomyces cerevisiae cell, a Schizosaccharomyces pombe cell, and a Pichia pastoris cell.
32. An isolated polypeptide comprising the amino acid sequence of an LXRα-64, LXRα-42e+, or
and LXRα-42e-.
33. The isolated polypeptide of claim 32, wherein the polypeptide comprises the amino acid
sequence of SEQ ID N06, 8, 17, 19, a naturally-occurring allelic variant thereof, or a fragment
thereof.

34. The isolated polypeptide of claim 32, wherein the polypeptide consists of the amino acid
sequence of SEQ ID NO:, 6,8, 17, 19, or a fragment thereof that does not share homology with
more than five contiguous amino acids of SEQ ID NO:2.
35. The polypeptide of claim 32, further comprising heterologous amino acid sequences.
36. A method for detecting the presence of a polypeptide of claim 32 in a sample, the method
comprising:

a) contacting the sample with a compound that selectively binds to a polypeptide of claim
32; and
b) determining whether the compound binds to the polypeptide in the sample.

37. The method of claim 37, wherein the compound that binds to the polypeptide is an antibody.
38. A kit comprising a compound that selectively binds to a polypeptide of claim 32 and
instructions for use.
39. An antibody that specifically binds the isolated polypeptide of claim 14 or claim 32.
40. The antibody of claim 39, wherein the antibody is a polyclonal antibody.
41. The antibody of according to claim 39, wherein the antibody is a monoclonal antibody.
42. The antibody of claim 39, wherein the antibody comprises a detectable label.

43. A composition comprising the antibody of claim 39 and a pharmaceutically acceptable
carrier.
44. A method of identifying a new LXRα variant nucleic acid molecule, the method comprising

(a) hybridizing a sample comprising one or more nucleic acid molecules with an LXRα
variant nucleic acid molecule or a fragment thereof under stringent hybridization
conditions;
(b) identifying a nucleic acid molecule in the sample that hybridizes with the LXRα variant
nucleic acid molecule, thereby identifying a putative LXRα variant nucleic acid
molecule; and
(c) determining the sequence of the putative LXRα variant nucleic acid molecule, wherein
a putative LXRα variant nucleic acid molecule having a sequence that is not identical
to the sequence of an LXRα variant is a new LXRα variant nucleic acid.

45. The method of claim 44, wherein the new LXRα variant nucleic acid molecule encodes an
LXRα variant polypeptide.
46. The method of claim 45, wherein the new LXRα variant polypeptide comprises one or more
conservative substitutions compared to an LXRα variant polypeptide.
47. A method of detecting expression of an LXRα variant in a biological sample, the method
comprising :

(a) hybridizing the biological sample with the nucleic acid molecule of claim 1; and
(b) determining whether the nucleic acid molecule hybridizes to a nucleic acid molecule in
the sample, wherein hybridization indicates that the LXRα variant is expressed.

48. The method of claim 47, wherein the amount of hybridization is determined.
49. A method of decreasing RXR dimer formation in a cell, the method comprising contacting the
cell with a polypeptide of claim 10, thereby inhibiting RXR dimer formation.
50. The method of claim 49, wherein RXR heterodimerization is inhibited.
51. The method of claim 49, wherein RXR homodimerization is inhibited.
52. A method of identifying an LXRα variant ligand, the method comprising

(a) providing a sample comprising an LXRα variant polypeptide,
(b) contacting the sample with a test compound,
(c) determining whether the test compound can bind to the LXRα variant, wherein a
compound that can bind to the LXRα variant is an LXRα variant ligand.

53. The method of claim 52, wherein the Kd of the ligand is less than 1 x 106.
54. The method of claim 52, wherein the Kd of the ligand is less than 1 x 109.
55. The method of claim 52, wherein an RXR is present in the sample.
56. The method of claim 52, further comprising determining whether the LXRα variant ligand can
bind a wild type LXRα.

57. The method of claim 52, wherein the LXRα variant ligand does not bind to a wild type LXRα.
58. The method of claim 52, wherein the LXRα variant ligand has a higher affinity for an LXRα
variant compared to a wild type LXRα.
59. A method of modulating the expression of an LXRα-regulated gene, the method comprising
modulating expression or activity of an LXRα variant.
60. The method of claim 59, wherein the LXRα-regulated gene is an FAS, CYP7A1 (cholesterol
7-alpha hydroxylase), ApoE, CETP (cholesterol ester transfer protein), LPL (lipoprotein lipase),
ABCG1, ABCG5, ABCG8, ABCG4, or PLTP (phospholipid transfer protein).

61. The method of claim 59, wherein the LXRα-regulated gene is an SREBP-1C (sterol regulatory
binding element 1c), FAS, CYP7A1 (cholesterol 7-alpha hydroxylase), ApoE, CETP (cholesterol ester
transfer protein), LPL (lipoprotein lipase), ABCA1 (ATP-binding cassette transporter-1), ABCG1,
ABCG5, ABCG8, ABCG4, or PLTP (phospholipid transfer protein).
62. The method of claim 59, wherein expression of the LXRα-regulated gene is increased.

63. The method of claim 59, wherein expression of the LXRα-regulated gene is decreased.
64. The method of claim 59, wherein the LXRα variant is an LXRα-64, LXRα-42+, or LXRα-42".
65. A method of modulating LXRα variant expression or activity in a subject, the method
comprising
introducing into a subject an LXRα variant nucleic acid molecule or a fragment thereof in an
amount and for a time sufficient for the LXRα variant to be expressed and modulate LXRα
expression or activity.
66. The method of claim 65, wherein the LXRα variant inhibits expression or activity of a wild-
type LXRα.
67. The method of claim 65, wherein the activity is LXRα heterodimerization.
68. The method of claim 66, wherein the LXRα heterodimerization is ligand stimulated.
69. The method of claim 68, wherein the LXRα variant is an LXRα-64, LXRα-42+, or LXRα-42".
70. A method of modulating expression or activity of an RXR in a subject, the method comprising
introducing into a subject an LXRα variant nucleic acid molecule or a fragment thereof in an
amount and for a time sufficient for the LXRα variant to be expressed and modulate expression or
activity of the RXR.

71. The method of claim 70, wherein heterodimerization of the RXR is modulated or
homodimerization of the RXR is modulated.
72. The method of claim 70, wherein, the heterodimerization of RXR with a PPARα, PPARγ,
PPAR8, RAR, XR, or PXR is modulated.
73. The method of claim 70, wherein RXR heterodimerization is inhibited or RXR
homodimerization is inhibited.
74. The method of claim 70, wherein the LXRα variant is an LXRα-64, LXRα-42+, or LXRα-42'.
75. The method of claim 70, wherein the RXR is an RXR, RXR, or RXRγ.
76. A method for treating an individual having an RXR-related disease or disorder, the method
comprising administering to the individual a pharmaceutically effective amount of an LXRα variant.
77. A pharmaceutical composition comprising the cell of claim 23 and a pharmaceutically
acceptable carrier, the isolated nucleic acid molecule of claim 1 and a pharmaceutically acceptable
carrier, or a polypeptide of claim 10 and a pharmaceutically acceptable carrier.

The present invention discloses an isolated nucleic acid molecule encoding a human liver X
receptor alpha (LXRα) variant polypeptide selected from the group consisting of:
(a) an isolated nucleic acid molecule encoding SEQ ID NO: 6, 8, 17, or 19;
(b) an isolated nucleic acid molecule encoding an amino acid sequence having at least 90% identity with the SEQ ID NO:, 6, 8, 17, or 19;
(c) an isolated nucleic acid molecule that hybridizes with the isolated nucleic acid
molecule of (a) or (b) under hybridization conditions of 6X SSC (1M NaCI), 50 % formamide, 1 % SDS at 42 °C, and a wash in 1X SSC at 42°C, and a wash at 68°C, in 0.2XSSC, and 0.1% SDS; and
(d) an isolated nucleic acid molecule that is complementary to (a), (b), or (c).