DESC:FIELD OF THE INVENTION:

The present invention relates to synergistic pesticidal composition comprising component (A) Flubendiamide component (B) at least one Strobilurin fungicides selected from from Trifloxystrobin, Azoxystrobin and component (C) at least one more fungicide (triazole) selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole, Tricyclazole. The present invention also relates to the method of preparing the said composition, comprising component (A) Flubendiamide component (B) at least one Strobilurin fungicide from Trifloxystrobin, Azoxystrobin and component (C) at least one more triazole fungicide selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole, and Tricyclazole. The present invention also provides a method of controlling or preventing pathogenic damage or pest damage in a plant propagation material, a plant, parts of a plant and/or plant organs that grow at a later point in time with the use of the present pesticidal composition.

BACKGROUND OF THE INVENTION

The control of phytopathogenic fungi is of great economic importance since fungal growth on plants or on parts of plants, e.g., fruits, blossoms, foliage, stems, tubers, roots, etc., inhibits production of foliage, fruit or seed and reduces the overall quantity and quality of the harvested crop. The continuous economic toll taken by phytopathogenic fungi establishes a continuing need to develop new, more effective fungicides which possess curative, preventative and systemic action to protect cultivated plants and the seeds from which they develop. Those requirements must be accomplished without any significant adverse side effects to the plants of the desired crops.

Flubendiamide (IUPAC name: 3-iodo-N’-(2-mesyl-1,1-dimethylethyl)-N-{4-[1,2,2,2-tetrafluoro-1- trifluoromethyl)ethyl] -o-tolyl} phthalamide ) Molecular formula: C23H22F7IN2O4S is the compound which was evaluated as a new compound by the 2010 JMPR for both residues and toxicological aspects. It is generally white crystalline powder which has melting point 217.5-220.7? C. Flubendiamide is a benzenedicarboxamide derivative that shows selective insecticidal activity against lepidopterous insects. The specific modulatory effects of flubendiamide on ryanodine binding in insect muscle microsomal membranes suggest that the ryanodine receptor (RyR) Ca(2+) release channel is a primary target of flubendiamide.

Flubendiamide activates ryanodine sensitive intracellular calcium release channels.
Ryanodine receptors are intracellular Ca2+ channels specialized for the rapid and massive release of Ca2+ from intracellular stores, which is an essential step in the muscle contraction process.

Ryanodine receptors (RyRs) are a distinct class of ligand gated calcium channels controlling the release of calcium from intracellular stores. They are located on the sarcoplasmic reticulum of muscle and the endoplasmic reticulum of neurons and many other cell types. Ryanodine, a plant alkaloid and an important ligand used to characterize and purify the receptor, has served as a natural botanical insecticide, but attempts to generate synthetic commercial analogues of ryanodine have proved unsuccessful. Recently /the phthalic acid and diamide/ classes of synthetic chemicals have emerged resulting in commercial insecticides that target insect RyRs.

It acts by targeting the ryanodine cell receptor and interfering with the calcium release channel, which is involved in muscle contraction. It is known to stabilize insect ryanodine receptors in an open state in a species specific manner and to desensitize the calcium dependence of channel activity. Continuous stimulation of muscle contraction by "locking" the calcium channel in an "open" state, leads to muscle paralysis and eventual death of the organism. Whole organism symptoms may include feeding cessation, lethargy, paralysis, and death.

Flubendiamide disrupts proper muscle function in insects and represents therefore a unique and novel mode of action.

The use of flubendiamide as an active ingredient was disclosed in US20100310518 A1 where in Flubendiamide was combined to other Beneficial Species from the orders or suborders of the Araneae, Acari, Dermaptera, Hymenoptera, Coleoptera, Neuroptera, Thysanoptera, Heteroptera, Diptera, Hemiptera, Dermaptera and/or Parasitiformes or at least one bacteria strain or at least one virus strain for the effective control of unwanted pests. This limits the treatment of the above invention to only insects. The above invention does not prevent the infection by pathogenic fungi.

US1816441 and US1105642 also mentions about the combination of insecticide and fungicide. However the composition of insecticide and fungicide in the patents is different.

In the present invention the active ingredient Flubendiamide is an insecticide. It is used in combination comprising of component (A) Flubendiamide component (B) at least one Strobilurin fungicide from Trifloxystrobin, Azoxystrobin and component (C) at least one more triazole fungicide selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole, Tricyclazole

Strobilurin Fungicides such as Trifloxystrobin and Azoxystrobin acts on the mitochondrial electron transport chain and thus interfering with respiration in plant pathogenic fungi, mitochondrial respiration pathway and fungal spore germination.
The fungicide group, demethylation inhibitors (DMI), which contain the triazole fungicides like Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole and Tricyclazole are also present in combination with the present active ingredient. These fungicides are highly effective against many different fungal diseases, especially powdery mildews, rusts, and many leaf-spotting fungi. The triazole fungicides inhibit one specific enzyme, C14-demethylase, which plays a role in sterol production. Sterols, such as ergosterol, are needed for membrane structure and function, making them essential for the development of functional cell walls. Therefore, these fungicides result in abnormal fungal growth and eventually death.
Each triazole compound may act in a slightly different part of the biochemical sterol-producing pathway. While the results are similar in various fungi--abnormal fungal growth and death--there are great differences in the activity spectra of these fungicides.
Triazoles have no effect against spore germination because spores contain enough sterol for the formation of germ tubes. Some spores even have enough sterol to produce infection structures so, in some cases, triazoles may not be effective against infection of the host tissue.

In general use, the pesticide actives are used in the form of a dilute aqueous composition because it can attain a good interaction with the target organism, such as plants, fungi and insects. However, most active pesticide compounds that are used as pesticides are only sparingly or even insoluble in water. The low solubility of such compounds present the challenges and difficulties to formulator in formulating pesticide compounds in stable formulations that can be easily stored for a long time and which still have a high stability and effective activity until end use.

Pest prevention and control issues are most prone to drug resistance, followed by a single control spectrum, many times when farmers use pesticide, causing serious environmental pollution, large field crop pesticides on human health. Applicants different fungicides and insecticides mechanism complex to achieve the purpose of preventing a double-jet, effective prevention and treatment of various diseases of crops, prevention of the original single agent to expand the spectrum, does not directly act on the crop and the crop growing period decomposition of pesticide residues on crop residue or no residue, by the food safety, and significant synergistic effects, excellent environmental compatibility, greatly reducing the amount of pesticide treatment, reducing the cost of pesticide.

For the reasons mentioned above there does a need to provide further formulation of combinations comprising component (A) Flubendiamide component (B) at least one Strobilurin fungicides selected from from Trifloxystrobin, Azoxystrobin and component (C) at least one more triazole fungicide selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole, Tricyclazole. This object is achieved according to the invention by providing the present formulation.

SUMMARY OF THE INVENTION

Accordingly, in a first aspect, the present invention provides a synergistic pesticidal composition comprising component (A) Flubendiamide component (B) at least one Strobilurin fungicides from Trifloxystrobin, Azoxystrobin and component (C) at least one more triazole fungicide selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole, Tricyclazole and one or more inactive excipients.

Accordingly, in a second aspect, the present invention provides a method of preparing the said synergistic pesticidal composition and one or more inactive excipients.

Accordingly, in a third aspect, the present invention provides a method of protecting a plant propagation material, a plant, parts of a plant and/or plant organs that grow at a later point in time against pathogenic damage or pest damage by applying to the plant propagation material a composition comprising a formulated mixture defined in the first aspect.

Accordingly, in a fourth aspect the said synergistic composition is to be developed in suitable formulations selected from Suspension Concentrate (SC) or Water Dispersible Granules (WG) comprising component (A) Flubendiamide component (B) at least one Strobilurin fungicides from Trifloxystrobin, Azoxystrobin and component (C) at least one more triazole fungicide selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole, Tricyclazole and optionally one or inactive excipients such as a) dispersant b) wetting agent c) anti foaming agent d) biocides e) anti freezing agent f) suspending agent g) thickener h) coating agent and i) buffering agent.

As per one more aspect, the suspension concentrate (SC) pesticidal composition comprising a) 1 to 40% Flubendiamide; b) 1% to 30% Strobilurin fungicides selected from Trifloxystrobin, Azoxystrobin; c) 1% to 30% triazole fungicide selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole and Tricyclazole

As per preferred embodiment, the composition of present invention is in the form of Suspension concentrate (SC) and/or water dispersible granules (WG)

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to pesticidal composition comprising component (A) Flubendiamide component (B) at least one Strobilurin fungicides from Trifloxystrobin, Azoxystrobin and component (C) at least one more triazole fungicide selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole and Tricyclazole. for the effective and environmentally friendly control of animal pests such as insects and/or lepidopterous insects and phytopathogenic fungi, e.g., such genera of the classes Ascomycetes, Basidiomycetes, Oomycetes, Fungi Imperfecti and Deuteromycetes as Rhizoctonia, Fusarium, Aspergillus, Penicillium, Ustilago, Tilletia, and so forth. More particularly, the invention herein concerns a composition containing the endospores of a microorganism capable of providing protection from phytopathogenic fungi and a chemical fungicide which is toxic to the microorganism.
One typical problem arising in the field of pest control lies in the need to reduce the dosage rates of the active ingredient in order to reduce or avoid unfavourable environmental or toxicological effects whilst still allowing effective pest and pathogen control.

Another problem encountered concerns the need to have available pest control agents which are effective against a broad spectrum of pests and pathogens.

Another problem underlying the present invention is the desire for compositions that improve plants, a process which is commonly and hereinafter referred to as “plant health”. For example, advantageous properties that may be mentioned are improved crop characteristics including: emergence, crop yields, protein content, more developed root system (improved root growth), tillering increase, increase in plant height, bigger leaf blade, less dead basal leaves, stronger tillers, greener leaf color, pigment content, photosynthetic activity, less fertilizers needed, less seeds needed, more productive tillers, earlier lowering, early grain maturity, less plant verse (lodging), increased shoot growth, enhanced plant vigor, increased plant stand and early germination; or any other advantages familiar to a person skilled in the art.

The invention furthermore relates to plant-protecting active ingredient mixtures having synergistically enhanced action and to a method of improving the health of plants by applying said mixtures to the plants or the locus thereof, for example a plant or a plant propagation material, that is infested or liable to be infested by fungi with the pesticides present in a inventive mixture in any desired sequence or simultaneously, that is, jointly or separately.

The present invention relates to pesticidal composition comprising component (A) Flubendiamide component (B) at least one Strobilurin fungicides from Trifloxystrobin, Azoxystrobin and component (C) at least one more triazole fungicide selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole, and Tricyclazole in ratios by weight of the active ingredients are 1% to 40% Flubendiamide 1% to 30% strobilurin fundicides and triazole fungicides 1% to 30%.

The novel active ingredient mixtures have very advantageous curative, preventive and systemic fungicidal properties for protecting cultivated plants. As has been mentioned, said active ingredient mixtures can be used to inhibit or destroy the pathogens that occur on plants or parts of plants (fruit, blossoms, leaves, stems, tubers, roots) of different crops or useful plants, while at the same time those parts of plants which grow later are also protected from attack by such pathogens. Active ingredient mixtures have the special advantage of being highly active against diseases in the soil that mostly occur in the early stages of plant development.

Specifically, the said synergistic compositions are suitable for controlling the following harmful insects: lepidopterans (Lepidoptera), for example Agrotisypsilon, Agrotissegetum, Alabama argillacea, Anticarsiagemmatalis, Argyresthiaconjugella, Autographa gamma, Bupaluspiniarius, Cacoeciamurinana, Capua reticulana, Cheimatobiabrumata, Choristoneurafumiferana, Choristoneuraoccidentalis, Cirphisunipuncta, Cydiapomonella, Dendrolimuspini, Diaphanianitidalis, Diatraeagrandiosella, Eariasinsulana, Elasmopalpuslignosellus, Eupoeciliaambiguella, Evetriabouliana, Feltiasubterranea, Galleria mellonella, Grapholithafunebrana, Grapholithamolesta, Heliothisarmigera, Heliothisvirescens, Heliothiszea, Hellulaundalis, Hibernia defoliaria, Hyphantriacunea, Hyponomeutamalinellus, Keiferialycopersicella, Lambdinafiscellaria, Laphygmaexigua, Leucopteracoffeella, Leucopterascitella, Lithocolletisblancardella, Lobesiabotrana, Loxostegesticticalis, Lymantriadispar, Lymantriamonacha, Lyonetiaclerkella, Malacosomaneustria, Mamestrabrassicae, Orgyiapseudotsugata, Ostrinianubilalis, Panolisflammea, Pectinophoragossypiella, Peridromasaucia, Phalerabucephala, Phthorimaeaoperculella, Phyllocnistiscitrella, Pierisbrassicae, Plathypenascabra, Plutellaxylostella, Pseudoplusiaincludens, Rhyacioniafrustrana, Scrobipalpula absolute, Sitotrogacerealella, Sparganothispilleriana, Spodopterafrugiperda, Spodopteralittoralis, Spodopteralitura, Thaumatopoeapityocampa, Tortrixviridana, Trichoplusiani and Zeiraphera Canadensis; Beetles (Coleoptera), for example Agrilussinuatus, Agrioteslineatus, Agriotesobscurus, Amphimallussolstitialis, Anisandrusdispar, Anthonomusgrandis, Anthonomuspomorum, Atomarialinearis, Blastophaguspiniperda, Blitophagaundata, Bruchusrufimanus, Bruchuspisorum, Bruchuslentis, Byctiscusbetulae, Cassidanebulosa, Cerotoma trifurcate, Ceuthorrhynchusassimilis, Ceuthorrhynchusnapi, Chaetocnematibialis, Conoderusvespertinus, Criocerisasparagi, Diabroticalongicornis, Diabroticaspeciosa, Diabrotica 12-punctata, Diabroticavirgifera, Diloboderusabderus, Epilachnavarivestis, Epitrixhirtipennis, Eutinobothrusbrasiliensis, Hylobiusabietis, Hyperabrunneipennis, Hyperapostica, Ipstypographus, Lemabilineata, Lemamelanopus, Leptinotarsadecemlineata, Limoniuscalifornicus, Lissorhoptrusoryzophilus, Melanotuscommunis, Meligethesaeneus, Melolonthahippocastani, Melolonthamelolontha, Oulemaoryzae, Ortiorrhynchussulcatus, Oryazophagusoryzae, Otiorrhynchusovatus, Phaedoncochleariae, Phyllotretachrysocephala, Phyllophaga sp., Phyllophagacuyabana, Phyllophagatriticophaga, Phylloperthahorticola, Phyllotretanemorum, Phyllotretastriolata, Popillia japonica, Sitonalineatus and Sitophilusgranaria, Dipterans (Diptera), for example Aedesaegypti, Aedesvexans, Anastrephaludens, Anopheles maculipennis, Ceratitiscapitata, Chrysomyabezziana, Chrysomyahominivorax, Chrysomyamacellaria, Contariniasorghicola, Cordylobiaanthropophaga, Culexpipiens, Dacuscucurbitae, Dacusoleae, Dasineurabrassicae, Fanniacanicularis, Gasterophilusintestinalis, Glossinamorsitans, Haematobiairritans, Haplodiplosisequestris, Hylemyiaplatura, Hypodermalineata, Liriomyzasativae, Liriomyzatrifolii, Lucille caprina, Luciliacuprina, Luciliasericata, Lycoriapectoralis, Mayetiola destructor, Muscadomestica, Muscinastabulans, Oestrusovis, Oscinella frit, Pegomyahysocyami, Phorbiaantiqua, Phorbiabrassicae, Phorbiacoarctata, Rhagoletiscerasi, Rhagoletispomonella, Tabanusbovinus, Tipulaoleracea and Tipulapaludosa, Thrips (Thysanoptera), e.g. Frankliniellafusca, Frankliniellaoccidentalis, Frankliniellatritici, Scirtothripscitri, Thripsoryzae, Thripspalmi and Thripstabaci, Hymenopterans (Hymenoptera), e.g. Acromyrmexambuguus, Acromyrmexcrassispinus, Acromyrmexheiery, Acromyrmexlandolti, Acromyrmexsubterraneus, Athaliarosae, Atta capiguara, Atta cephalotes, Atta laevigata, Atta robusta, Atta sexdens, Atta texana, Hoplocampaminuta, Hoplocampatestudinea, Monomoriumpharaonis, Solenopsis geminate and Solenopsisinvicta, Heteropterans (Heteroptera), e.g. Acrosternumhilare, Blissusleucopterus, Cyrtopeltisnotatus, Dichelopsfurcatus, Dysdercuscingulatus, Dysdercusintermedius, Euchistosheros, Eurygasterintegriceps, Euschistusimpictiventris, Leptoglossusphyllopus, Lyguslineolaris, Lyguspratensis, Nezaraviridula, Piesmaquadrata, Piezodorusguildini, Solubeainsularis and Thyantaperditor, Hemiptera and Homoptera, e.g. Acrosternumhilare, Blissusleucopterus, Cyrtopeltisnotatus, Diaphorinacitri, Dysdercuscingulatus, Dysdercusintermedius, Eurygasterintegriceps, Euschistusimpictiventris, Leptoglossusphyllopus, Lyguslineolaris, Lyguspratensis, Nezaraviridula, Piesmaquadrata, SolubeainsularisThyantaperditor, Acyrthosiphononobrychis, Adelgeslaricis, Aphidulanasturtii, Aphis fabae, Aphis forbesi, Aphis pomi, Aphis gossypii, Aphis grossulariae, Aphis schneideri, Aphis spiraecola, Aphis sambuci, Acyrthosiphonpisum, Aulacorthumsolani, Brachycauduscardui, Brachycaudushelichrysi, Brachycauduspersicae, Brachycaudusprunicola, Brevicorynebrassicae, Capitophorushorni, Cerosiphagossypii, Chaetosiphonfragaefolii, Cryptomyzusribis, Dreyfusianordmannianae, Dreyfusiapiceae, Dysaphisradicola, Dysaulacorthumpseudosolani, Dysaphisplantaginea, Dysaphispyri, Empoascafabae, Hyalopteruspruni, Hyperomyzuslactucae, Macrosiphumavenae, Macrosiphumeuphorbiae, Macrosiphonrosae, Megouraviciae, Melanaphispyrarius, Metopolophiumdirhodum, Myzodespersicae, Myzusascalonicus, Myzuscerasi, Myzusvarians, Nasonoviaribis-nigri, Nilaparvatalugens, Pemphigus bursarius, Perkinsiellasaccharicida, Phorodonhumuli, Psyllamali, Psyllapiri, Rhopalomyzusascalonicus, Rhopalosiphummaidis, Rhopalosiphumpadi, Rhopalosiphuminsertum, Sappaphis mala, Sappaphismali, Schizaphisgraminum, Schizoneuralanuginosa, Sitobionavenae, Trialeurodesvaporariorum, Toxopteraaurantiiand, Viteusvitifolii, Cimexlectularius, Cimexhemipterus, Reduviussenilis, Triatoma spp., and Ariluscritatus, Termites (Isoptera), e.g. Calotermesflavicollis, Cornitermescumulans, Heterotermestenuis, Leucotermesflavipes, Neocapritemesopacus, Procornitermestriacifer; Reticulitermeslucifugus, Syntermesmolestus, and Termesnatalensis, Orthopterans (Orthoptera), e.g. Achetadomestica, Blattaorientalis, Blattellagermanica, Forficulaauricularia, Gryllotalpagryllotalpa, Locustamigratoria, Melanoplusbivittatus, Melanoplus femur-rubrum, Melanoplusmexicanus, Melanoplussanguinipes, Melanoplusspretus, Nomadacrisseptemfasciata, Periplanetaamericana, Schistocercaamericana, Schistocercaperegrina, Stauronotusmaroccanus and Tachycinesasynamorus, Arachnoidea, such as arachnids, e.g. of the families Argasidae, Ixodidae and Sarcoptidae, such as Amblyommaamericanum, Amblyommavariegatum, Argaspersicus, Boophilusannulatus, Boophilusdecoloratus, Boophilusmicroplus, Dermacentorsilvarum, Hyalommatruncatum, Ixodesricinus, Ixodesrubicundus, Ornithodorusmoubata, Otobiusmegnini, Dermanyssusgallinae, Psoroptesovis, Rhipicephalusappendiculatus, Rhipicephalusevertsi, Sarcoptesscabiei, and Eriophyidae spp. such as Aculusschlechtendali, Phyllocoptrataoleivora and Eriophyessheldoni; Tarsonemidae spp. such as Phytonemuspallidus and Polyphagotarsonemuslatus; Tenuipalpidae spp. such as Brevipalpusphoenicis; Tetranychidae spp. such as Tetranychuscinnabarinus, Tetranychuskanzawai, Tetranychuspacificus, Tetranychustelarius and Tetranychusurticae, Panonychusulmi, Panonychuscitri, and oligonychuspratensis; Thrips (Thysanoptera), e.g. Frankliniellafusca, Frankliniellaoccidentalis, Frankliniellaschultzei, Frankliniellatritici, Scirtothripscitri, Thripsoryzae, Thripspalmi and Thripstabaci.

The compositions according to the invention have very good fungicidal properties and can be employed for controlling phytopathogenic fungi such as Ascomycetes, Basidiomycetes, Chytridiomycetes, Deuteromycetes, Oomycetes, Plasmodiophoromycetes, Zygomycetes, and the like. Examples which may be mentioned, but not by limitation, are some pathogens of fungal diseaseswhich come under the above generic terms:
Diseases caused by pathogens causing powdery mildew such as, for example, Blumeria species such as, for example, Blumeria graminis; Podosphaera species such as, for example, Podosphaera leucotricha; Sphaerotheca species such as, for example, Sphaerotheca fuliginea; Uncinula species such as, for example, Uncinula necator; Leveillula species such as, for example Leveillula taurica, Erysiphe species such as for example Erysiphe polygoni, diseases caused by pathogens of rust diseases such as, for example, Gymnosporangium species such as, for example, Gymnosporangium sabinae, Hemileia species such as, for example, Hemileia vastatrix; Phakopsora species such as, for example, Phakopsora pachyrhizi and Phakopsora meibomiae; Puccinia species such as, for example, Puccinia graminis, Puccinia recondita or Puccinia triticina, Puccinia striiformis; Uromyces species such as, for example, Uromyces phaseoli; diseases caused by pathogens of smut diseases such as, for example, Sporisorium species such as , for example, Sporisorium scitamineum; Ustilago species such as, for example Ustilago maydis, Tilletia species such as for example Tilletia tritici, Ustilaginoidea species such as , for example Ustilaginoidea virens, diseases caused by pathogens of ergot diseases such as, for example Claviceps species, Claviceps purpurea; diseases caused by pathogens from the group of the Oomycetes such as, for example, Bremia species such as, for example, Bremia lactucae; Peronospora species such as, for example, Peronospora pisi or P. brassicae; Phytophthora species such as, for example, Phytophthora infestans; Plasmopara species such as, for example, Plasmopara viticola; Pseudoperonospora species such as, for example, Pseudoperonospora humuli or Pseudoperonospora cubensis; Pythium species such as, for example, Pythium ultimum; leaf spot diseases and leaf wilt caused by, for example, Alternaria species such as, for example, Alternaria solani; Cercospora species such as, for example, Cercospora beticola; Cladiosporum species such as, for example, Cladiosporium cucumerinum; Cochliobolus species such as, for example, Cochliobolus sativus (conidial form: Drechslera, syn: Helminthosporium); Colletotrichum species such as, for example, Colletotrichum lindemuthanium; Cycloconium species such as, for example, Cycloconium oleaginum; Diaporthe species such as, for example, Diaporthe citri; Elsinoe species such as, for example, Elsinoe fawcettii; Gloeosporium species such as, for example, Gloeosporium laeticolor; Glomerella species such as, for example, Glomerella cingulata; Guignardia species such as, for example, Guignardia bidwelli; Leptosphaeria species such as, for example, Leptosphaeria maculans; Magnaporthe species such as, for example, Magnaporthe grisea; Mycosphaerella species such as, for example, Mycosphaerella graminicola; Phaeosphaeria species such as, for example, Phaeosphaeria nodorum; Pyrenophora species such as, for example, Pyrenophora teres; Ramularia species such as, for example, Ramularia collo-cygni; Rhynchosporium species such as, for example, Rhynchosporium secalis; Septoria species such as, for example, Septoria apii; Typhula species such as, for example, Typhula incarnata; Venturia species such as, for example, Venturia inaequalis; root and stalk diseases, caused by, for example, Corticium species such as, for example, Corticium graminearum; Fusarium species such as, for example, Fusarium oxysporum; Gaeumannomyces species such as, for example, Gaeumannomyces graminis; Rhizoctonia species such as, for example, Rhizoctonia solani; Tapesia species such as, for example, Tapesia acuformis; Thielaviopsis species such as, for example, Thielaviopsis basicola; ear and panicle diseases (including maize cobs), caused by, for example, Alternaria species such as, for example, Alternaria spp.; Aspergillus species such as, for example, Aspergillus flavus; Cladosporium species such as, for example, Cladosporium spp.; Claviceps species such as, for example, Claviceps purpurea; Fusarium species such as, for example, Fusarium culmorum; Gibberella species such as, for example, Gibberella zeae; Monographella species such as, for example, Monographella nivalis; diseases caused by smuts such as, for example, Sphacelotheca species such as, for example, Sphacelotheca reiliana; Tilletia species such as, for example, Tilletia caries; Urocystis species such as, for example, Urocystis occulta; Ustilago species such as, for example, Ustilago nuda; fruit rot caused by, for example, Aspergillus species such as, for example, Aspergillus flavus; Botrytis species such as, for example, Botrytis cinerea; Penicillium species such as, for example, Penicillium expansum; Sclerotinia species such as, for example, Sclerotinia sclerotiorum; Verticilium species such as, for example, Verticilium alboatrum; seed- and soil-borne rots and wilts, and seedling diseases, caused by, for example, Fusarium species such as, for example, Fusarium culmorum; Phytophthora species such as, for example, Phytophthora cactorum; Pythium species such as, for example, Pythium ultimum; Rhizoctonia species such as, for example, Rhizoctonia solani; Sclerotium species such as, for example, Sclerotium rolfsii; cankers, galls and witches' broom diseases, caused by, for example, Nectria species such as, for example, Nectria galligena; wilts caused by, for example, Monilinia species such as, for example, Monilinia laxa; deformations of leaves, flowers and fruits, caused by, for example, Taphrina species such as, for example, Taphrina deformans; degenerative diseases of woody species, caused by, for example, Esca species such as, for example, Phaemoniella clamydospora; flower and seed diseases, caused by, for example, Botrytis species such as, for example, Botrytis cinerea; diseases of plant tubers caused by, for example, Rhizoctonia species such as, for example, Rhizoctonia solani; diseases caused by bacterial pathogens such as, for example, Xanthomonas species such as, for example, Xanthomonas campestris pv. oryzae; Pseudomonas species such as, for example, Pseudomonas syringae pv. lachrymans; Erwinia species such as, for example, Erwinia amylovora; by preference, the following diseases of soya beans can be controlled: fungal diseases on leaves, stems, pods and seeds caused by, for example, alternaria leaf spot (Alternaria spec, atrans tenuissima), anthracnose (Colletotrichum gloeosporoides dematium var. truncatum), brown spot (Septoria glycines), cercospora leaf spot and blight (Cercospora kikuchii), choanephora leaf blight (Choanephora infundibulifera trispora (Syn.)), dactuliophora leaf spot (Dactuliophora glycines), downy mildew (Peronospora manshurica), drechslera blight (Drechslera glycini), frogeye leaf spot (Cercospora sojina), leptosphaerulina leaf spot (Leptosphaerulina trifolii), phyllostica leaf spot (Phyllosticta sojaecola), powdery mildew (Microsphaera diffusa), pyrenochaeta leaf spot (Pyrenochaeta glycines), rhizoctonia aerial, foliage, and web blight (Rhizoctonia solani), rust (Phakopsora pachyrhizi), scab (Sphaceloma glycines), stemphylium leaf blight (Stemphylium botryosum), target spot (Corynespora cassiicola) fungal diseases on roots and the stem base caused by, for example, black root rot (Calonectria crotalariae), charcoal rot (Macrophomina phaseolina), fusarium blight or wilt, root rot, and pod and collar rot (Fusarium oxysporum, Fusarium orthoceras, Fusarium semitectum, Fusarium equiseti), mycoleptodiscus root rot (Mycoleptodiscus terrestris), neocosmospora (Neocosmopspora vasinfecta), pod and stem blight (Diaporthe phaseolorum), stem canker (Diaporthe phaseolorum var. caulivora), phytophthora rot (Phytophthora megasperma), brown stem rot (Phialophora gregata), pythium rot (Pythium aphanidermatum, Pythium irregulare, Pythium debaryanum, Pythium myriotylum, Pythium ultimum), rhizoctonia root rot, stem decay, and damping-off (Rhizoctonia solani), sclerotinia stem decay (Sclerotinia sclerotiorum), sclerotinia southern blight (Sclerotinia rolfsii), thielaviopsis root rot (Thielaviopsis basicola).

The composition according to the invention can be applied to any and all developmental stages of pests, such as egg, larva, pupa, and adult. The pests may be controlled by contacting the target pest, its food supply, habitat, breeding ground or its locus with a pesticidally effective amount of the composition.

The synergistic pesticidal composition of the present is very helpful to the farmers to protect following crops from insect-pest and fungal diseases as their incidence always coincide at a time;

Crop Insect-Pests Fungal diseases
Paddy/ Rice Stem borer (Scirpophaga incertulas Walker), Leaf folder (Cnaphalocrocis medinalis) Sheath Blight (Rhizoctonia solani), Blast (Pyricularia oryza), Helminothosporium leaf spot, False smut, (Ustilaginoidea virens), Grains discoloration (Curvularia spp., Helminthosporium spp., Drechslera spp.)
Wheat Pink Stem borer (Sesamia inferens), Spodoptera litura Rust (Puccinia striiformis), Powdery mildew (Blumeria graminis), Grain discoloration, Head blight (Fusarium spp.), Septoria leaf spot
Maize Shoot borer (Chilo partellus), Corn worm (Helicoverpa armigera) Leaf blight (Helminthosporium spp., Drechshlera spp., Bipolaris turcicum)
Chilli Fruit borer complex (Helicovera armigera, Spodoptera litura, Spodoptera exigua) Die back (Colletotrichus capsici), Fruit rot (Phytophthora capsici), Leaf spot (Cercospora spp., and Alternaria spp.)
Tomato Fruit borer (Helicoverpa armigera) Early Blight (Alternaria solani), Late blight (Phytophthora spp.)
Brinjal Fruit & Shoot borer (Leucinoides orbonalis) Leaf blight (Phoma spp.,), Leaf spot (Alternaria spp., Cercospora spp.)
Cumin Spodoptera litura, Helicoverpa armigera Blight (Alternaria spp.), Powdery mildew (Erysiphe spp.)
Onion Spodoptera litura & Helicoverpa armigera Purple blotch (Alternaria porri), Stemphylium Leaf blight, Black mould (Aspergillus spp.)
Cole crop Plutella xylostella, Spodoptera litura Alternaria leaf spot, Downy mildew (Peronospora spp.)

Accordingly, in one embodiment a synergistic pesticidal composition comprises a pesticidally effective amount of component (A) Flubendiamide, component (B) at least one Strobilurin fungicide from Trifloxystrobin, Azoxystrobin and component (C) at least one more triazole fungicide selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole, and Tricyclazole optionally, along with at least one agriculturally acceptable adjuvant and/or carrier. A “pesticidally effective amount” is an amount of active ingredient or a combination of active ingredients that is effective to prevent or reduce damage to a plant caused by any pest or to repel, deter or destroy a pest or to cause an adverse effect to an individual insect or an insect population, including, for example, deviations from natural development, killing, regulation, and the like. Suitable adjuvants or carriers should not be phytotoxic to valuable crops, particularly at the concentrations employed in applying the compositions for pest control in the presence of crops, and should not react chemically with pesticidal components of the mixture or other composition ingredients.

Examples of suitable solid carriers include talc, pyrophyllite clay, silica, attapulgus clay, kaolin clay, kieselguhr, chalk, diatomaceous earth, lime, calcium carbonate, bentonite clay, Fuller's earth, cottonseed hulls, wheat flour, soybean flour, pumice, wood flour, walnut shell flour, lignin, and the like or mixtures thereof.

Inactive excipients, or adjuvants, that are often used in agricultural compositions, and that can be included in various compositions described herein, include, but are not limited to, dispersing agents, wetting agent, antifoam agents, biocide, anti-freezing agent, suspension aid, thickening agents (also referred to as “thickeners”), quick coating agent or sticking agents (also referred to as “stickers” or “binders”),

A dispersing agent is a substance which adsorbs onto the surface of particles and helps to preserve the state of dispersion of the particles and prevents them from re-aggregating. Dispersing agents are added to agrochemical formulations to facilitate dispersion and suspension during manufacture, and to ensure the particles re-disperse into water in a spray tank. They are widely used in wettable powders, suspension concentrates and water-dispersible granules. Surfactants that are used as dispersing agents have the ability to adsorb strongly onto a particle surface and provide a charged or steric barrier to re-aggregation of particles. The most commonly used surfactants are anionic, non-ionic, or mixtures of the two types. For wettable powder formulations, the most common dispersing agents are sodium lignosulphonates. For suspension concentrates, very good adsorption and stabilization are obtained using polyelectrolytes, such as sodium naphthalene sulphonate formaldehyde condensates. Tristyrylphenolethoxylate phosphate esters are also used. Non-ionics such as alkylarylethylene oxide condensates and EO-PO block copolymers are sometimes combined with anionics as dispersing agents for suspension concentrates. In recent years, new types of very high molecular weight polymeric surfactants have been developed as dispersing agents. These have very long hydrophobic ‘backbones’ and a large number of ethylene oxide chains forming the ‘teeth’ of a ‘comb’ surfactant. These high molecular weight polymers can give very good long-term stability to suspension concentrates because the hydrophobic backbones have many anchoring points onto the particle surfaces. Examples of dispersing agents used herein include but not limited to sodium lignosulphonates; sodium naphthalene sulphonateformaldehyde condensates; tristyrylphenolethoxylate phosphate esters; aliphatic alcohol ethoxylates; alky ethoxylates; EO-PO block copolymers; and graft copolymers or mixtures thereof.

A wetting agent is a substance that when added to a liquid increases the spreading or penetration power of the liquid by reducing the interfacial tension between the liquid and the surface on which it is spreading. Wetting agents are used for two main functions in agrochemical formulations: during processing and manufacture to increase the rate of wetting of powders in water to make concentrates for soluble liquids or suspension concentrates; and during mixing of a product with water in a spray tank or other vessel to reduce the wetting time of wettable powders and to improve the penetration of water into water-dispersible granules. Examples of wetting agents used in wettable powder, suspension concentrate, and water-dispersible granule formulations include but not limited to Trisiloxane ethoxylate, sodium lauryl sulphate; sodium dioctylsulphosuccinate; alkyl phenol ethoxylates; and aliphatic alcohol ethoxylates or mixtures thereof.

As per preferred embodiment, the wetting agent is Trisiloxane ethoxylate. One more preferred embodiment, Trisiloxane ethoxylate is present in the range from 0.2 to 20%.

Anti-foam agents generally, are of two types of anti-foam agents, namely silicones and non-silicones. Silicones are usually aqueous emulsions of dimethyl polysiloxane while the non-silicone anti-foam agents are water-insoluble oils, such as octanol and nonanol, or silica. In both cases, the function of the anti-foam agent is to displace the surfactant from the air-water interface.

Biocides / Microorganisms cause spoilage of formulated products. Therefore preservation agents are used to eliminate or reduce their effect. Examples of such agents include, but are not limited to: propionic acid and its sodium salt; sorbic acid and its sodium or potassium salts; benzoic acid and its sodium salt; p-hydroxy benzoic acid sodium salt; methyl p-hydroxy benzoate; and biocide such as sodium benzoate, 1,2-benzisothiazoline-3-one, 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, potassium sorbate, parahydroxy benzoates or mixtures thereof.

Anti-freezing agents may be selected from but not limited to glycerin, ethylene glycol, propylene glycol or mixtures thereof.

Suspension aid in the present description denotes a natural or synthetic, organic or inorganic material with which the active substance is combined in order to facilitate its application to the plant, to the seeds or to the soil. This carrier is hence generally inert, and it must be agriculturally acceptable, in particular to the plant being treated. The carrier may be solid (clays, natural or synthetic silicates, silica, resins, waxes, solid fertilizers, and the like or mixtures thereof) or liquid (water, alcohols, ketones, petroleum fractions, aromatic or paraffinic hydrocarbons, chlorinated hydrocarbons, liquefied gases, and the like or mixtures thereof).

Thickening, gelling, and anti-settling agents generally fall into two categories, namely water-insoluble particulates and water-soluble polymers. It is possible to produce suspension concentrate formulations using clays and silicas. Examples of these types of materials, include, but are limited to, montmorillonite, e.g. bentonite; magnesium aluminum silicate; and attapulgite. Water-soluble polysaccharides have been used as thickening-gelling agents for many years. The types of polysaccharides most commonly used are natural extracts of seeds and seaweeds are synthetic derivatives of cellulose or mixtures thereof. Examples of these types of materials include, but are not limited to, guar gum; locust bean gum; carrageenam; alginates; methyl cellulose; sodium carboxymethyl cellulose (SCMC); hydroxyethyl cellulose (HEC) or mixtures thereof. Other types of anti-settling agents are based on modified starches, polyacrylates, polyvinyl alcohol and polyethylene oxide or mixtures thereof. Another good anti-settling agent is xanthan gum.

Quick coating are silicones such as Silicon tri-alkoxylate, dimethicone 350, dimethicone 360, dimethiconecopolyol, cyclomethicone, silicon dioxide, silicone, simethicone, colloidal silicone, poly(dimethylsiloxane/ methylvinylsiloxane/ methylhydrogensiloxane) dimethylvinyl or dimethylhydroxy or trimethyl end blocked, polydimethylsiloxanes, polydimethylsiloxane oils or polydimethylsiloxane oils modified with ionic or nonionic organic groups, a linear functional polydiorganosiloxane, a linear non-functional polydiorganosiloxane, a cyclic polydiorganosiloxane, an alkoxydimethicone, an alkyldimethicone, a phenyldimethicone, a silicone resin and mixtures thereof. More examples are dimethylpolysiloxane, methylphenylpolysiloxanes, cyclic silicones, and amino-, fatty acid-, alcohol-, polyether-, epoxy-fluoro- and/or alkyl-modified silicone compounds, and also polyalkylsiloxanes, polyalkylarylsiloxanes, polyethersiloxanes.

The dispersing agent are, for example polyesters, polyamides, poly- carbonates, polyurea and polyurethanes, acrylate polymers and copolymers, styrene copolymers, butadiene copolymers, polysaccharides such as starch and cellulose derivatives, vinylal- cohol, vinylacetate and vinylpyrrolidone polymers and copolymers, polyethers, epoxy, phenolic and melamine resins, polyolefins and define copolymers and mixtures thereof. Examples of preferred polymers are acrylate polymers such as poly(methacrylate), poly( ethyl methacrylate), poly(methylmethacrylate), acrylate copoylmers and styrene-acrylic copolymers as defined herein below , poly(styrene-co maleic anhydride), cellulosic polymers such as ethyl cellulose, cellulose acetate, cellulose acetatebutyrate, acetylated mono-, di-, and triglycerides, poly(vinylpyrrolidone), vinyl acetate polymers and copolymers, poly(alkylene glycol), styrene butadiene copolymers, poly(orthoesters), alkyd resins, and mixtures of two or more of these. Polymers that are biodegradable are also useful in the present invention. As used herein, a polymer is biodegradable if is not water soluble, but is degraded over a period of several weeks when placed in an application environment. Examples of biodegradable polymers that are useful in the present method include biodegradable polyesters, starch, polylactic acid -starch blends, polylactic acid, poly(lactic acid-glycolic acid) copolymers, polydioxanone, cellulose esters, ethyl cellulose, cellulose acetate butyrate, starch esters, starch ester - aliphatic polyester blends, modified corn starch, polycaprolactone, poly(n-amylmethacrylate), wood rosin, polyan- hydrides, polyvinylalcohol, polyhydroxybutyrate-valerate, biodegradable aliphatic polyesters, and polyhydroxybutyrate or mixtures thereof.

Pesticide compositions can be frequently applied as aqueous suspensions or emulsions prepared from concentrated formulations of such compositions. Such water-soluble, water-suspendable, or emulsifiable formulations are either solids, usually known as wettable powders, or water dispersible granules, or liquids usually known as emulsifiable concentrates, or aqueous suspensions. Wettable powders, which may be compacted to form water dispersible granules, comprise an intimate mixture of the pesticide composition, a carrier, and surfactants. The carrier is usually chosen from among the attapulgite clays, the montmorillonite clays, the diatomaceous earths, or the purified silicates. Effective surfactants, which can comprise from about 0.5% to about 10% of the wettable powder, are found among sulfonatedlignins, condensed naphthalenesulfonates, naphthalenesulfonates, alkylbenzenesulfonates, alkyl sulfates, and nonionic surfactants such as ethylene oxide adducts of alkyl phenols.

Aqueous suspensions comprise suspensions of water-insoluble pesticide compositions dispersed in an aqueous carrier. Suspensions are prepared by finely grinding the pesticide composition and vigorously mixing it into a carrier comprised of water and surfactants. Ingredients, such as inorganic salts and synthetic or natural gums, may also be added, to increase the density and viscosity of the aqueous carrier. It is often most effective to grind and mix the pesticide composition at the same time by preparing the aqueous mixture and homogenizing it in an implement such as a sand mill, ball mill, or piston-type homogenizer.

As per present invention the active ingredients are present in various concentrations as provided below;
Insecticide Strobilurin Fungicide Triazole Fungicide
% of components varies from Flubendiamide Trifloxystrobin Difenoconazole
Azoxystrobin Epoxiconazole
Flutriafol
Hexaconazole
Propiconazole
Prothioconazole
Tebuconazole
Tricyclazole
1%-40% 1%-30% 1%-30%

List of inactive agent which may be used in the present invention are listed below but not limited to:
Inactive excipients to be used in SC composition
Excipients Function
Acrylic graft copolymer, Alkylated naphthalene sulfonate, sodium salt, Sodium salt of naphthalene sulfonate condensate, Sodium ligno sulfonate Dispersing agent
Polyalkoxy alkyl ether, Ethylene oxide/propylene oxide block copolymer Dispersing agent, Emulsifier
Polyarylphenyl ether phosphate, Ethoxylated Fatty Alcohol Dispersing agent & Wetting agent
Trisiloxane ethoxylate Wetting agent & Spreader
Sodium dioctyl sulfosuccinate Wetting agent & Emulsifier
Silicone antifoam emulsion Antifoam
Polysaccharide Rheology Modifier,Thickner
Glycol (Monoethylene glycol, Diethylene glycol, Polypropylene glycol, Polyethylene glycol), Glycerene Antifreezing Agent
1,2-benzisothiazolin-3-one, Sodium benzoate, 2-bromo-2-nitropropane-1,3-diol, Formaldehyde, Sodium o-phenylphenate, 5-chloro-2-methyl-4-isothiazolin-3-one & 2-methyl-4-isothiazolin-3-one Biocide/Preservative
Aluminum Magnesium Silicate Suspending aid
Silicone dioxide Suspending aid, Anticaking agent
Polydimethyl siloxane Antifoaming agent
Organo modified Trisiloxane Spreader
Poly glycerol ester Sticker, Penetrant
Poly ether Deposition aid

Inactive excipients to be used in WG composition
Excipients Function
Sodium salt of naphthalene sulfonate condensate, Modified polyacrylate copolymer, Sodium polycarboxylate, Sodium Ligno sulfonate, Alkyl naphthalene sulfonate condensate, sodium salt, Dispersing Agent
Polyacrylate co-polymer Dispersing Agent, Stabilizer, Emulsifier
Alcohol block co-polymer Co-dispersant
Blend of naphthalene sulfonate condensate & phenol sulfonate condensate Wetting Agent & Dispersing Agent
blend of naphthalene sulfonate condensate, Sodium dodecylbenzene sulfonate, Sodium lauryl sulfate, Blend of alkyl naphthalene sulfonate and anionic wetting agent, Sodium n-butyl naphthalene sulfonate, Sodium isopropyl naphthalene sulfonate, Blend of sodium alkyl aryl sulfonates Wetting Agent
Corn starch Binder, Filler
Silicon dioxide Suspending agent, Anti-caking Agent, Filler
Urea formaldehyde resin / Urea-methanol Anti caking agent
Lactose, China clay/ Kaolin, Titanium dioxide, Talcum powder, Diatomaceous earth Filler
Polyvinylpyrrolidone Binder
Silicone antifoam emulsion Defoaming agent
Fatty amine ethoxylate Wetting agent, Dispersing agent, Stabilizers, Sanitizers, Defoaming agent

As per one embodiment, the preferred composition with % concentration of active ingredient and composition type are as mentioned below;
Sr. No. Active Ingredient (A) Active Ingredient (B) Active Ingredient
(C) (%) in formulation Preferred Formulation type Formulation per Hectare (g or ml)
(A) (B) (C)
1 Flubendiamide Trifloxystrobin Hexaconazole 8.00% 20.00% 5.00% SC 625
2 Flubendiamide Trifloxystrobin Hexaconazole 12.00% 30.00% 7.50% WG 416.67
3 Flubendiamide Trifloxystrobin Tebuconazole 5.00% 10.00% 14.00% SC 1250
4 Flubendiamide Trifloxystrobin Tebuconazole 10.00% 20.00% 28.00% WG 625
5 Flubendiamide Trifloxystrobin Prothioconazole 5.00% 8.00% 12.00% SC 1250
6 Flubendiamide Trifloxystrobin Prothioconazole 10.00% 16.00% 24.00% WG 625
7 Flubendiamide Trifloxystrobin Epoxiconazole 8.00% 13.33% 6.70% SC 750
8 Flubendiamide Trifloxystrobin Epoxiconazole 16.00% 26.70% 13.33% WG 375
9 Flubendiamide Trifloxystrobin Tricyclazole 8.00% 20.00% 28.00% WG 625
10 Flubendiamide Trifloxystrobin Difenoconazole 8.00% 16.00% 8.00% SC 750
11 Flubendiamide Trifloxystrobin Difenoconazole 12.00% 24.00% 12.00% WG 500
12 Flubendiamide Trifloxystrobin Flutriafol 8.00% 16.00% 8.00% SC 750
13 Flubendiamide Trifloxystrobin Propiconazole 8.00% 16.00% 8.00% SC 750
14 Flubendiamide Azoxystrobin Hexaconazole 8.00% 20.00% 5.00% SC 625
15 Flubendiamide Azoxystrobin Hexaconazole 12.00% 30.00% 7.50% WG 400
16 Flubendiamide Azoxystrobin Tebuconazole 6.00% 9.00% 15.00% SC 1000
17 Flubendiamide Azoxystrobin Tebuconazole 12.00% 18.00% 30.00% WG 500
18 Flubendiamide Azoxystrobin Prothioconazole 6.00% 9.00% 15.00% SC 1000
19 Flubendiamide Azoxystrobin Prothioconazole 12.00% 18.00% 30.00% WG 500
20 Flubendiamide Azoxystrobin Epoxiconazole 8.00% 12.00% 6.70% SC 750
21 Flubendiamide Azoxystrobin Epoxiconazole 16.00% 24.00% 13.33% WG 375
22 Flubendiamide Azoxystrobin Tricyclazole 8.00% 14.33% 28.00% WG 625
23 Flubendiamide Azoxystrobin Difenoconazole 8.00% 12.00% 8.00% SC 750
24 Flubendiamide Azoxystrobin Difenoconazole 16.00% 24.00% 16.00% WG 375
25 Flubendiamide Azoxystrobin Flutriafol 8.00% 12.00% 8.00% SC 750
26 Flubendiamide Azoxystrobin Propiconazole 6.00% 7.50% 12.50% SC 1000

The pesticidal compositions according to the invention are applied to the plants mainly by spraying (over the top) by manually operated knap sack sprayer, power sprayer or machine operated sprayer. Here, the application can be carried out using, for example, water as carrier by customary spraying techniques using spray liquor amounts of from about 50 to 1000 l/ha (for example from 200 to 600 l/ha). The compositions according to the present invention can be applied at before or after insect-pest and fungal diseases infection (i.e. as a preventive, curative or eradicative).
Moreover, it may be advantageous to apply the compositions of the present invention on their own or jointly in combination with other crop protection agents, for example with agents for controlling bacteria. Also of interest is the miscibility with mineral salt solutions which are employed for treating nutritional and trace element deficiencies.
The amounts of active substances applied, i.e. active ingredient A, B and C without formulation auxiliaries / excipients , are depending on the kind of effect desired, from 0.1 to 10000 grams per hectare (g/ha), preferably from 1 to 1000 g/ha, more preferably from 10 to 500 g/ha and in particular from 20 to 250 g/ ha.
According to the invention the amount of formulated product applied depending upon the kind of effect desired, from 1 to 10000 ml or g per hectare, preferable from 10 to 5000 ml or g per hectare, more preferably from 100 to 1500 ml or g per hectare.
The synergistic pesticidal composition of the present invention is offering many advantages compared to conventional composition available as provided below;
• Increase in yield of treated plants (cereals, pulses, oilseeds, fiber crop, sugar crops, leafy vegetables, tuber crops, fruit crops, flowers, ornamentals etc.). Increase in yield means increased plant weight, increased plant height, increased biomass such as higher overall fresh weight (FW), increased number of flowers per plant, higher grain yield, more tillers or side shoots (branches), larger leaves, increased shoot growth, increased protein content, increased oil content, increased starch content, increased pigment content,
• Increase in yield due to protection against Insect-pest damage and fungal diseases
• Increase in yield due to plant growth regulation, check vegetative growth and increase in reproductive parts of plant.
• Increase in yield due to more number of tillers, more branches and sub branches, more number of fruits, flowers, and grains size.
• Increase plant vigor
• Increase tolerance to insect-pests and fungal damage
• Increase tolerance to the weather stress, moisture stress and heat stress
• Prevents lodging in susceptible plants (lodging due to biotic and abiotic factors, like heavy rains, winds, insects and diseases damage.
• Improves quality (means visual appearance, color, size, shape etc.) in grains, fruits, fiber, flowers, tuber, bulb, rhizomes, straw, leaves and other plant parts and plant products
• Improves keeping quality of produce, increase post harvest life, storage life, protection from post harvest diseases
• Uniform sizing in tuber, bulb, rhizome and root crops.
As per one embodiment the synergistic pesticidal composition is in the form of Suspension Concentrate and Water Dispersible Granules. As per one more preferred embodiment, the Suspension concentrate contains wetting agent along with other inactive excipients. As a wetting agent when Trisiloxane ethoxylate is used in the composition, the final composition exhibits stable and effective composition and if the suspension concentrate (SC) is prepared without Trisiloxane ethoxylate, the final composition do not complies as per desired specification including stability and other parameters.

Thus as per analysis, it was found that Trisiloxane ethoxylate plays an important role in preparing synergistic pesticidal composition of component (A) Flubendiamide component (B) at least one Strobilurin fungicides from Trifloxystrobin, Azoxystrobin and component (C) at least one more triazole fungicide selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole, Tricyclazole.

The process for preparing the present agrochemical composition can be modified accordingly by any person skilled in the art based on the knowledge of the manufacturing the composition. However all such variation and modification is still covered by the scope of present invention.

EXAMPLES

Example 1: Flubendiamide 8%+Trifloxystrobin 20%+Hexaconazole 5% SC
Chemical Composition Percent (% w/w)
Flubendiamide a.i. 8.0
Trifloxystrobin a.i. 20.0
Hexaconazole a.i. 5.0
Ethoxylated Fatty Alcohol 2.0
Acrylic graft copolymer 3.0
Alkylated naphtalene sulfonate, sodium salt 0.5
Silicone antifoam 0.5
1,2-benzisothiazolin-3-one 0.20
Mono Ethylene Glycol 5.0
Polysaccharides 0.15
Trisiloxane ethoxylate 2.0
Water QS
TOTAL 100.0

Procedure:
(i) Take required quantity of water, biocide, and defoamer and homogenize, then slowly add gum powder to it and stir till complete dissolution. Preparing Gum Solution 12 to 18 hours prior to use.
(ii) Charging required quantity of Demineralised water (DM) water which is needed to be taken in designated vessel for Flowable concentrate/ Suspension concentrate/ Flowable slurry production.
(iii) Adding required quantity of Wetting agent, dispersing agent & suspending agents, colourant/dye and homogenize the contents for 45 to 60 minutes using high shear homogenizer.
(iv) Adding technical and other remaining adjuvants excluding ‘antifreeze & thickeners’ and homogenized to get uniform slurry ready for grinding.
(v) Before grinding half the quantity of antifoam was added and then the material was subjected to three cycles of grinding in Dyno mill.
(vi) The half quantity of the antifoam was added along with antifreeze after grinding process completes and before sampling for in process analysis.
(vii) Finally add gum solution to this formulation and send to QC for quality check.

Storage stability
Parameters Specification (In house) Initial Stability (for 14 days)
At 54±2 0C At 0±2 0C
Description Off White colour flowable liquid Complies Complies Complies
Flubendiamide content percent by mass 7.6 to 8.8 8.20 8.16 8.18
Trifloxystrobin content percent by mass 19 to 21 20.34 20.21 20.27
Hexaconazole content percent by mass 4.75 to 5.5 5.12 5.05 5.07
Flubendiamide suspensibility percent mini. 80 98.81 97.15 98.68
Trifloxystrobin suspensibility percent mini. 80 97.05 95.89 96.55
Hexaconazole suspensibility percent mini. 80 96.85 95.01 95.75
pH range (1% aq. Suspension) 5.5 to7.5 6.9 6.7 6.9
Pourability 95 % min 98.50 97.70 97.60
Specific gravity 1.05 – 1.15 1.07 1.08 1.07
Viscosity at spindle no.62, 20 rpm 350 -800 cps 410 425 430
Particle size (micron) D50 <3, D90 <10 2.2, 7.9 2.4, 8.1 2.7, 8.5
Persistent foam ml (after 1 minute) max. 60 5 5 5

Room temperature storage data
Parameters Specification (In house) Study Duration
1 month 6 month 12 months
Description Off White colour flowable liquid Complies Complies Complies
Flubendiamide content percent by mass 7.6 to 8.8 8.21 8.20 8.15
Trifloxystrobin content percent by mass 19 to 21 20.31 20.27 20.23
Hexaconazole content percent by mass 4.75 to 5.5 5.10 5.09 5.09
Flubendiamide suspensibility percent mini. 80 97.20 97.05 96.20
Trifloxystrobin suspensibility percent mini. 80 97.05 96.50 94.35
Hexaconazole suspensibility percent mini. 80 95.80 95.10 93.20
pH range (1% aq. Suspension) 5.5 to7.5 6.9 6.8 6.6
Pourability 95 % min 98.50 98.40 97.20
Specific gravity 1.05 – 1.15 1.07 1.07 1.08
Viscosity at spindle no.62, 20 rpm 350 -800 cps 410 415 490
Particle size (micron) D50 <3, D90 <10 2.2, 8 2.2, 8.1 2.8, 8.9
Persistent foam ml (after 1 minute) max. 60 5 5 5

Example 1.A: Flubendiamide 8%+Trifloxystrobin 20%+Hexaconazole 5% SC
Chemical Composition Percent (% w/w)
Flubendiamide a.i. 8.0
Trifloxystrobin a.i. 20.0
Hexaconazole a.i. 5.0
Ethoxylated Fatty Alcohol 2.0
Polyalkoxy alkylether 3.5
Alkylated naphtalene sulfonate, sodium salt 1.5
Polydimethyl Siloxane 0.5
Formaldehyde 0.20
Mono Ethylene Glycol 5.0
Polysaccharides 0.15
Water QS
TOTAL 100.0

Procedure: As per Example 1
Storage stability:
Parameters Specification (In house) Initial Stability (for 14 days)
At 54±2 0C At 0±2 0C
Description Off White colour flowable liquid Complies Complies Complies
Flubendiamide content percent by mass 7.6 to 8.8 8.15 8.05 8.09
Trifloxystrobin content percent by mass 19 to 21 20.25 20.13 20.19
Hexaconazole content percent by mass 4.75 to 5.5 5.20 5.17 5.09
Flubendiamide suspensibility percent mini. 80 88.20 87.30 82.50
Trifloxystrobin suspensibility percent mini. 80 85.20 83.50 82.70
Hexaconazole suspensibility percent mini. 80 87.20 79.50 81.20
pH range (1% aq. Suspension) 5.5 to7.5 7.1 6.5 6.2
Pourability 95 % min 97.50 91.20 93.20
Specific gravity 1.05 – 1.15 1.07 1.08 1.07
Viscosity at spindle no.62, 20 rpm 350 -800 cps 445 713 820
Particle size (micron) D50 <3, D90 <10 2.7, 7.9 4.2, 13.5 3.9, 11.4
Persistent foam ml (after 1 minute) max. 60 35 29 30

Room temperature storage
Parameters Specification (In house) Study Duration
1 month 6 month 12 months
Description Off White colour flowable liquid Complies Complies Complies
Flubendiamide content percent by mass 7.6 to 8.8 8.25 8.13 8.11
Trifloxystrobin content percent by mass 19 to 21 20.50 20.29 20.21
Hexaconazole content percent by mass 4.75 to 5.5 5.21 5.13 5.12
Flubendiamide suspensibility percent mini. 80 97.50 95.20 91.50
Trifloxystrobin suspensibility percent mini. 80 97.10 91.30 83.50
Hexaconazole suspensibility percent mini. 80 95.70 91.20 85.10
pH range (1% aq. Suspension) 5.5 to7.5 7.1 7.3 6.4
Pourability 95 % min 96.50 92.70 91.50
Specific gravity 1.05 – 1.15 1.07 1.07 1.08
Viscosity at spindle no.62, 20 rpm 350 -800 cps 450 510 1810
Particle size (micron) D50 <3, D90 <10 2.7,8.5 3.8,9.5 6.2,15.5
Persistent foam ml (after 1 minute) max. 60 35 40 35

Example 2: Flubendiamide 12%+Trifloxystrobin 30%+Hexaconazole 7.5% WG
Chemical Composition Percent (% w/w)
Flubendiamide a.i. 12.0
Trifloxystrobin a.i. 30.0
Hexaconazole a.i. 7.5
Alkylated naphthalene sulfonate, sodium salt 6.0
Sodium salt of Phenolsulfonic condensate 3.0
Polyacrylate polymer sodium salt 2.5
Silicone antifoam 1.0
Lactose 14.1
Corn starch 13.0
China Clay QS
TOTAL 100.0

Procedure:
Step 1 Charge the required quantity of filler, wetting agent, dispersing agent, and suspending agent, & technical in premixing blender for homogenization for 30 minutes.
Step 2 Pre-blended material is then grinded through Jet mill/ air classifier mills. Finely grinded material is blended in post blender till it becomes homogeneous. (for approx 1.5 hr)
Step 3 Finely grinded powder is mixed with required quantity of water to form extrudable dough.
Step 4 Dough is passed through extruder to get granules of required size.
Step 5 Wet granules are passed through Fluidized bed drier and further graded using vibrating screens.
Step 6 Final product is sent for QC approval.
Step 7 After approval material is packed in required pack sizes.

Storage stability
Parameters Specification (In house) Initial Stability (for 14 days)
At 54±2 0C At 0±2 0C
Description Off white colour granules Complies Complies Complies
Flubendiamide content percent by mass 11.4 to 12.6 12.20 12.07 12.18
Trifloxystrobin content percent by mass 28.5 to 31.5 30.35 30.12 30.33
Hexaconazole content percent by mass 7.125 to 8.25 7.59 7.52 7.58
Flubendiamide suspensibility percent min. 70 96.80 95.50 96.10
Trifloxystrobin suspensibility percent min. 70 94.50 92.81 94.38
Hexaconazole suspensibility percent min. 70 95.80 94.18 95.08
pH range (1% aq. Suspension) 5.5 to7.5 7.2 7.1 7.1
Wettability sec. max. 60 8 9 9
Wet Sieve(45 micron ) percent by mass min. 98.5 99.6 99.3 99.5
Bulk density (g/ml) 0.45 to 0.75 0.50 0.51 0.50
Moisture content percent by mass max. Max. 2.0% 1.2 0.8 1

Room Temperature storage data
Parameters Specification (In house) Study Duration
1 month 6 month 12 months
Description Off white colour granules Complies Complies Complies
Flubendiamide content percent by mass 11.4 to 12.6 12.19 12.17 12.14
Trifloxystrobin content percent by mass 28.5 to 31.5 30.32 30.28 30.23
Hexaconazole content percent by mass 7.125 to 8.25 7.59 7.58 7.56
Flubendiamide suspensibility percent min. 70 96.66 96.12 95.76
Trifloxystrobin suspensibility percent min. 70 94.41 94.22 93.87
Hexaconazole suspensibility percent min. 70 95.78 95.46 95.34
pH range (1% aq. Suspension) 5.5 to7.5 7.2 7.2 7.2
Wettability sec. max. 60 8 8 9
Wet Sieve(45 micron ) percent by mass min. 98.5 99.6 99.5 99.5
Bulk density (g/ml) 0.45 to 0.75 0.5 0.5 0.51
Moisture content percent by mass max. Max. 2.0% 0.8 0.7 0.6

Example 2.A: Flubendiamide 12%+Trifloxystrobin 30%+Hexaconazole 7.5% WG
Ingredient Percent (w/w)
Flubendiamide a.i. 12.0
Trifloxystrobin a.i. 30.0
Hexaconazole a.i. 7.5
Alkylated naphthalene sulfonate, sodium salt 6.0
Sodium Lauryl Sulfate 3.0
Polydimethyl Siloxane 1.0
Lactose 14.1
Corn starch 13.0
China Clay QS
TOTAL 100.0

Procedure: As per Example 2

Storage Stability data
Parameters Specification (In house) Initial Stability (for 14 days)
At 54±2 0C At 0±2 0C
Description Off white colour granules Complies Complies Complies
Flubendiamide content percent by mass 11.4 to 12.6 12.18 12.02 12.17
Trifloxystrobin content percent by mass 28.5 to 31.5 30.32 30.05 30.29
Hexaconazole content percent by mass 7.125 to 8.25 7.61 7.51 7.58
Flubendiamide suspensibility percent min. 70 91.22 85.20 82.90
Trifloxystrobin suspensibility percent min. 70 89.70 67.15 85.20
Hexaconazole suspensibility percent min. 70 81.50 67.59 80.20
pH range 5.5 to7.5 7.2 6.9 7.1
Wettability sec. max. 60 10 11 11
Wet Sieve(45 micron ) percent by mass min. 98.5 99.2 98.7 98.9
Bulk density (g/ml) 0.45 to 0.75 0.50 0.51 0.50
Moisture content percent by mass max. Max. 2.0% 1.8 0.9 1.7

Room Temperature Storage data
Parameters Specification (In house) Study Duration
1 month 6 month 12 months
Description Off white colour granules Complies Complies Complies
Flubendiamide content percent by mass 11.4 to 12.6 12.16 12.14 12.09
Trifloxystrobin content percent by mass 28.5 to 31.5 30.27 30.21 30.15
Hexaconazole content percent by mass 7.125 to 8.25 7.58 7.56 7.53
Flubendiamide suspensibility percent min. 70 90.88 88.73 86.01
Trifloxystrobin suspensibility percent min. 70 89.05 88.01 82.2
Hexaconazole suspensibility percent min. 70 80.32 78.59 75.32
pH range (1% aq. Suspension) 5.5 to7.5 7.2 7.2 7.1
Wettability sec. max. 60 10 10 11
Wet Sieve(45 micron ) percent by mass min. 98.5 99.00 98.80 98.20
Bulk density (g/ml) 0.45 to 0.75 0.5 0.5 0.51
Moisture content percent by mass max. Max. 2.0% 1.7 1.6 1.2

Example 3: Bio efficacy trials
The synergistic pesticide action of the inventive mixtures can be demonstrated by the experiments below. A synergistic effect exists wherever the action of a combination (ready-mix) of active ingredient is greater than the sum of the action of each of the components alone. Therefore a synergistically effective amount or an effective amount of a synergistic composition or combination is an amount that exhibits greater pesticide activity than the sum of the pesticide activities of the individual components. In the field of agriculture, it is often understood that the term “synergy” is as defined by Colby S.R. in an article entitled “ Calculation of the synergistic and antagonistic responses of herbicide combinations” published in the journal Weeds, 1967, 15, p.20-22, incorporated herein by reference in its entirety. The action expected for a given combination of two active components can be calculated as follows:
Colby’s Formula:

To study the synergistic effect of combination of flubendiamide, strobilurin and triazole, various sets of experiments were conducted in different crops like rice, tomato and chilli.
Trial 1: Synergism
The synergistic combination of Flubendiamide, Trifloxystrobin and Hexaconazole was evaluated on rice crop, which has been regarded as one of the most important cereal crops and major food grain contributor to the total world good grain basket. Rice productivity is influenced by many of the biotic and abiotic stresses. The key biotic stresses are like insect-pests, diseases and weeds. At present, there are very limited strategies are employed to combat insect pests, fungal diseases at a time. The objective of the present study was to provide inventive solution to the rice growers to control many insect pests and fungal diseases which are generally coincide. The incidence of key lepidopteran like stem borer and leaf folder coincides with blast, sheath blight and grain discoloration in major rice growing area of the country. Farmers do unscientific tank mixing of insecticides and fungicides which ultimately lead to product failure, pest resurgence and pest outbreak and resistance development which causes big losses to the rice growers.
Details of Experiment:
a) Experiment design : Randomized Block Design
b) Replication : Four
c) Treatments : Twelve
d) Plot size : 50 sq. m.
e) Spacing : 2 seedlings per hill
f) Test Crop & Variety : Rice, PR 1121
g) Time of application : Two spray. At at 25th and 50th DATP (Days After Transplanting)
h) Spray method : Manually operated knap sack sprayer, using 500 liter per hectare Water volume
Evaluation Method :
1) Sheath blight: Observed 20 hills per plot and visually rate the disease by following rating.

Score Description
0 No Infection
1 Vertical spread of diseases up to 20% of plant height
3 Vertical spread of diseases up to 21-30% of plant height
5 Vertical spread of diseases up to 31-45% of plant height
7 Vertical spread of diseases up to 46-65% of plant height
9 Vertical spread of diseases >66% of plant height

Calculate disease severity as

PDI (%) =. Sum of numerical rating x 100 .
Total no. of plants observed x Max. Grade
The percent disease reduction over Untreated Check (UTC) was calculated as below:
% Reduction over UTC = 100 - __ (100 x PDI % in Treatment) __
(PDI % in Untreated Check)
2) False smut: Observe all the panicles from 0.25 m2 (50 x 50 cm) area and count no. of smutted grains per 0.25 m2 area from 10 random spots per plot at mature grain stage. Present the data as No. of smutted grains per m2. Then calculate % Disease reduction over UTC as per the formula given above.
3) Grain Discoloration: Observe 100 panicles per plot and count the no. of Healthy & Infected grains per panicles at mature grain stage. Calculate % Grain Discoloration and then calculate % Disease reduction over UTC as per the formula given above.
4) Stem borer damage :
Dead heart symptom: Count the number of dead heart per hill and record observations from 20 hills per plot. Calculate % dead heart. Convert the % dead heart data in to % reduction over control. Crop stage 3 to 5.
% Dead heart = Number of dead heart or infested tillers X 100.
Number of total tillers observed
Whitehead count: Count the number of whitehead per hill and record observations from 20 hills per plot. Calculate % whitehead. Convert the % whitehead data in to % reduction over control. Crop Stage 7 to 9
% Whitehead = Number of whitehead or infested tillers with panicles X 100.
Number of total tillers with panicles observed
5) Leaf folder damage: Count the number of healthy leaflet and damage leaflet per hill. Record observations from 20 hills per plot. Convert % Leaf folder data into % reduction over control. Calculate % Leaf folder damage.
% Leaf folder damage = Number of infested leaflet per hill X 100.
Total number of leaflet per hill
6) 1000 grain weight: Randomly take 10 samples from harvested grains of each plot at the time of harvest. Each samples with counted number of 1000 grains should be weighed and take average weight.
7) Effective Tillers: Count the number of tillers bearing panicle at the growth stage of grain filling from 1 sq.mt spot. Take such 5 spot per plot.
8) Plant Lodging: Record % of plant lodged from the entire plot by visual observation at the growth stage of mature grain.
9) Yield: Record the grain weight in gram from 5 sq. m spot per plot and convert it to quintal per acre at the time of harvest.
Table 1 Diseases control in Rice, Oryza sativa L.
Treatment details % Disease reduction over control
Sheath Blight, Rhizoctonia solani False smut, Ustilaginoidea virens Grain Discoloration
OV CV Ratio OV CV Ratio OV CV Ratio
Flub+Trifloxy+Hexa,50+125+31.25 gai/h 100 72.31 1.38 100 81.09 1.23 100 72.18 1.39
Flub+Trifloxy+Tricy,50+125+175 gai/h 100 72.28 1.38 100 81.14 1.23 100 68.89 1.45
Flub+Trifloxy,50+125 gai/h 61.08 56.18 1.09 63.12 68.42 0.92 53.28 57.48 0.93
Flub+Hexa,50+31.25 gai/h 32.24 36.82 0.88 37.82 40.12 0.94 32.64 34.58 0.94
Flub+Tricy,50+175 gai/h 32.54 36.74 0.89 39.20 40.28 0.97 25.96 26.84 0.97
Trifloxy+Hexa,125+31.25 gai/h 77.26 72.31 1.07 84.16 81.09 1.04 75.60 72.18 1.05
Trifloxy+Tricy,125+175 gai/h 75.18 72.28 1.04 80.92 81.14 1.00 70.64 68.89 1.03
Flub, 50 gai/h 0.00 0.00 0.00
Trifloxy, 125 gai/h 56.18 68.42 57.48
Hexa, 31.25 gai/h 36.82 40.12 34.58
Tricy, 175 gai/h 36.74 40.28 26.84
Untreated Check (UTC) 0.00 0.00 0.00
Where Flub- Flubendiamide, Trifloxy- Trifloxystrobin, Hex-Hexaconazole, Tricy-Tricyclazole, g.a.i/h- gram active ingredient per hectare, OV- Observed Value, CV- Calculated Value, Ratio- Synergism OV/CV. Sheath blight caused by Rhizoctonia solani, False smut by Ustilaginoidea virens, Grains discoloration by Curvularia spp., Helminthosporium spp., Drechslera spp.

The results of above experiment shows that inventive synergistic combination of Flubendiamide + Trifloxystrobin+ Hexaconazole and Flubendiamide+ Trifloxystrobin+ Tricyclazole shows superior control of rice diseases like sheath blight, false smut and grain discoloration, compared to their two way mixtures (prior arts) as well as their individual application.
Table 2: Insect control in Rice
Treatment details % reduction over control
Dead heart due to Stem borer Whitehead due to Stem borer Leaf folder damage
OV CV Ratio OV CV Ratio OV CV Ratio
Flub+Trifloxy+Hexa,50+125+31.25 gai/h 100 98.25 1.02 100 96.14 1.04 100 95.64 1.05
Flub+Trifloxy+Tricy,50+125+175 gai/h 100 98.25 1.02 100 96.14 1.04 100 95.64 1.05
Flub+Trifloxy,50+125 gai/h 97.28 98.24 0.99 95.26 96.13 0.99 96.12 95.63 1.01
Flub+Hexa,50+31.25 gai/h 95.64 98.24 0.97 94.82 96.13 0.99 95.62 95.63 1.00
Flub+Tricy,50+175 gai/h 94.28 98.24 0.96 96.72 96.13 1.01 94.28 95.63 0.99
Trifloxy+Hexa,125+31.25 gai/h 0.2 0.40 0.50 0.20 0.40 0.50 0.20 0.40 0.50
Trifloxy+Tricy,125+175 gai/h 0.2 0.40 0.50 0.2 0.40 0.50 0.2 0.40 0.50
Flub, 50 gai/h 98.24 96.12 95.62
Trifloxy, 125 gai/h 0.20 0.20 0.20
Hexa, 31.25 gai/h 0.20 0.20 0.20
Tricy, 175 gai/h 0.20 0.20 0.20
Untreated Check (UTC) 0.00 0.00 0.00
Where Flub- Flubendiamide, Trifloxy- Trifloxystrobin, Hex-Hexaconazole, Tricy-Tricyclazole, g.a.i/h- gram active ingredient per hectare, OV- Observed Value, CV- Calculated Value, Ratio- Synergism OV/CV. Dead heart and whitehead symptoms caused stem borer (Scirpophaga incertulas Walker) and leaf folder (Cnaphalocrocis medinalis).

The inventive synergistic combination of Flubendiamide+ Trifloxystrobin+ Hexaconazole and Flubendiamide+ Trifloxystrobin+ Tricyclazole provides excellent control of dead heart and whitehead caused by stem borer and leaf folder respectively compared to their two way mixtures (prior art) as well as their individual application.

Table 3: Effective tillers
Treatment details Number of effective tillers % Increase over Untreated
Flub+Trifloxy+Hexa,50+125+31.25 gai/h 43.26 57.08
Flub+Trifloxy+Tricy,50+125+175 gai/h 43.10 56.50
Flub+Trifloxy,50+125 gai/h 36.82 33.70
Flub+Hexa,50+31.25 gai/h 34.20 24.18
Flub+Tricy,50+175 gai/h 33.82 22.80
Trifloxy+Hexa,125+31.25 gai/h 33.66 22.22
Trifloxy+Tricy,125+175 gai/h 33.62 22.08
Flub, 50 gai/h 32.18 16.85
Trifloxy, 125 gai/h 31.24 13.44
Hexa, 31.25 gai/h 29.74 7.99
Tricy, 175 gai/h 28.48 3.41
Untreated Check (UTC) 27.54 0.00
Where Flub- Flubendiamide, Trifloxy- Trifloxystrobin, Hex-Hexaconazole, Tricy-Tricyclazole, and g.a.i/h- gram active ingredient per hectare, OV- Observed Value, CV- Calculated Value, Ratio- Synergism OV/CV.

The inventive combination of Flubendiamide + Trifloxystrobin + Hexaconazole and Flubendiamide + Trifloxystrobin + Tricyclazole produces maximum number of effective tillers compared to other tested combinations as well as solo application.

Table 4: Plant lodging at harvest
Treatments (g.a.i./h) % Lodging at time of harvest
Flub+Trifloxy+Hexa,50+125+31.25 gai/h 0.0
Flub+Trifloxy+Tricy,50+125+175 gai/h 0.0
Flub+Trifloxy,50+125 gai/h 10.0
Flub+Hexa,50+31.25 gai/h 10.0
Flub+Tricy,50+175 gai/h 10.0
Trifloxy+Hexa,125+31.25 gai/h 5.0
Trifloxy+Tricy,125+175 gai/h 5.0
Flub, 50 gai/h 20.0
Trifloxy, 125 gai/h 20.0
Hexa, 31.25 gai/h 15.0
Tricy, 175 gai/h 15.0
Untreated Check (UTC) 55.00
Where Flub- Flubendiamide, Trifloxy- Trifloxystrobin, Hex-Hexaconazole, Tricy-Tricyclazole, and g.a.i/h- gram active ingredient per hectare, OV- Observed Value, CV- Calculated Value, Ratio- Synergism OV/CV.

Plant lodging has been reduced by two sequential applications (at 25 and 50 days after transplanting) of inventive combination of Flubendiamide + Trifloxystrobin+ Hexaconazole and Flubendiamide + Trifloxystrobin + Tricyclazole which ultimately contributes to the reduction in grain quality and yield.

Table 5: 1000 Grain weight
Treatments (g.a.i./h) 1000 grain weight (g) % Increase over Untreated
Flub+Trifloxy+Hexa,50+125+31.25 gai/h 38.26 37.43
Flub+Trifloxy+Tricy,50+125+175 gai/h 38.12 36.93
Flub+Trifloxy,50+125 gai/h 37.28 33.91
Flub+Hexa,50+31.25 gai/h 36.14 29.81
Flub+Tricy,50+175 gai/h 34.84 25.14
Trifloxy+Hexa,125+31.25 gai/h 35.64 28.02
Trifloxy+Tricy,125+175 gai/h 35.22 26.51
Flub, 50 gai/h 30.16 8.33
Trifloxy, 125 gai/h 32.74 17.60
Hexa, 31.25 gai/h 31.64 13.65
Tricy, 175 gai/h 31.26 12.28
Untreated Check (UTC) 27.84 0.00
Where Flub- Flubendiamide, Trifloxy- Trifloxystrobin, Hex-Hexaconazole, Tricy-Tricyclazole, and g.a.i/h- gram active ingredient per hectare, OV- Observed Value, CV- Calculated Value, Ratio- Synergism OV/CV.

Table 6: Grain yield (quintal/acre)
Treatments (g.a.i./h) Quintal/acre % Increase over Untreated
Flub+Trifloxy+Hexa,50+125+31.25 gai/h 23.14 78.83
Flub+Trifloxy+Tricy,50+125+175 gai/h 22.86 76.66
Flub+Trifloxy,50+125 gai/h 18.76 44.98
Flub+Hexa,50+31.25 gai/h 18.64 44.05
Flub+Tricy,50+175 gai/h 17.98 38.95
Trifloxy+Hexa,125+31.25 gai/h 16.38 26.58
Trifloxy+Tricy,125+175 gai/h 16.12 24.57
Flub, 50 gai/h 15.62 20.71
Trifloxy, 125 gai/h 16.08 24.27
Hexa, 31.25 gai/h 14.28 10.36
Tricy, 175 gai/h 13.76 6.34
Untreated Check (UTC) 12.94 0.00
Where Flub- Flubendiamide, Trifloxy- Trifloxystrobin, Hex-Hexaconazole, Tricy-Tricyclazole, and g.a.i/h- gram active ingredient per hectare, OV- Observed Value, CV- Calculated Value, Ratio- Synergism OV/CV.

The yield data shows that inventive combination of Flubendiamide+Trifloxystrobin+ Hexaconazole and Flubendiamide+Trifloxystrobin+Tricyclazole produces higher grain yield, which is approximately > 75% compared to untreated.

Trial 2 Recipe comparison
Bioefficacy against Early blight and fruit borer in tomato crop
The innovative formulation (SC Suspension concentrate) of synergistic combination of Flubendiamide+Trifloxystrobin + Hexaconazole was developed and compared for its efficacy against fruit borer and early blight in Tomato (Solanum lycopericum) with many prior art and their solo use.
Details of Experiment:
a) Experiment design : Randomized Block Design
b) Replication : Four
c) Treatments : Nine
d) Plot size : 25 sq. m.
e) Spacing : 60 cm x 30 cm
f) Test Crop & Variety : Tomato, Himsikhar
g) Time of application : Two spray, 1st at 45 days, 2nd at 60 days after transplanting
h) Spray Volume : 1st spray- 375 l/h and 2nd spray-475 l/h
i) Method of application : Foliar spray with knap sack sprayer
The tomato nursery was raised and transplanted in the main field at row to row 60 cm and plant to plant 30 cm spacing. Rest of the agronomic practices like fertilizer applications, weeding, irrigation (drip irrigation) was as per the standard agronomic practices. The spraying was done at 45th and 60th days after transplanting with the help of knapsack sprayer. The observations on disease severity and incidence of early blight, fruit borer damage and fruit counts were taken as follow:
1) Early Blight:
The observations on early blight (caused by Alternaria solani): Disease severity was assessed by using following disease rating scale.
Score Symptoms
0 No symptom of disease on plant
1 Small, irregular brown spots covering 1% or less the leaf area
3 Small, irregular brown spots with concentric rings, covering 1-10% leaf area
5 Lesion enlarging, irregular brown spots with concentric rings, covering 11-25% leaf area
7 Lesions coalesce to form irregular dark brown patches with concentric rings, covering 26-50% of leaf area, Lesions on stem and petioles
9 Lesions coalesce to form irregular dark brown patches with concentric rings, covering more than 51% of leaf area, Lesions on stem and petioles

Sum of all disease rating x 100
Percent Disease Intensity (PDI) = ---------------------------------------------------------------
Total number of leaves assessed x Maximum rating

Number of Infected trifoliate leaves
Disease Incidence (%) = ----------------------------------------------------------------- X 100
Total number of trifoliate leaves observed

2) Fruit borer damage:
Count the number of healthy and damage fruits (caused by Helicoverpa armigera) per plant. Record such observations from 10 plants per plot, at 15 days after second spray. Calculate % fruit borer damage as:
Number of damaged fruits
Fruit borer damage (%) = ------------------------------------- X 100
Total number of fruits

Table 7: Treatment details
Treatment details Active Ingredient (g/h) Formulation (ml or g/h)

T1 : Recipe 1-Flubendiamide 8%+Trifloxystrobin
20%+Hexaconazole 5% SC 60+150+37.5 750
T2 : Recipe 2-Flubendiamide 8%+Trifloxystrobin
20%+Hexaconazole 5% SC 60+150+37.5 750
T3 : Prior Art 1-Flubendiamide 48% SC+
Trifloxystrobin 25% WG 60+150 125+600
T4 : Prior Art 2-Flubendiamide 48% SC+
Hexaconazole 5% SC 60+37.5 125+750
T5 : Prior Art 3-Trifloxystrobin 25% WG+
Hexaconazole 5% SC 150+37.5 600+750
T6 : Flubendiamide 48% SC 60 125
T7 : Trifloxystrobin 25% WG 150 600
T8 : Hexaconazole 5% SC 37.5 750
T9 : Untreated Check (UTC) 0 0

Table 8: Early blight disease and fruit borer control in Tomato (recipe comparison)
Treatment details Early Blight, Alternaria solani % Fruit damage by Helicoverpa armigera No. of healthy fruits per plant
Incidence (%) Severity (%)
T1 0.00 0.00 0.00 42.72
T2 5.12 8.64 2.64 37.64
T3 7.16 15.26 5.72 32.16
T4 7.86 18.64 6.28 31.38
T5 6.82 14.72 18.72 21.36
T6 18.84 43.94 6.56 29.46
T7 7.24 17.18 20.72 20.42
T8 8.86 23.26 23.48 19.88
T9 20.64 48.18 27.86 11.72
The field efficacy data on tomato trials shows that the innovative SC formulation (T1-Recipe 1) of Flubendiamide+ Trifloxystrobin +Hexaconazole gives excellent protection against early blight and tomato fruit borer. The early blight disease incidence and severity was higher in T2 (Recipe 2) and all prior art treatments compared to T1 (Recipe 1). T1 (Recipe1) also produces highest number of healthy tomato fruits. The treatment of T1 (Recipe 1), gave maximum protection to tomato due to excellent adhesion and retention, spreading properties, better penetration and minimal run off, which leads to increasing biological performance.

Example 2: Effect of seed treatment on Groundnut
A micro plot experiment was conducted on Groundnut (Arachis hypogaea) to study the synergism between Flubendiamide, Trifloxystrobin and Hexaconazole. The Groundnut seeds are treated and sown in the micro plot. The micro plot are filled with sick soil, soil inoculated with Rhizoctonia spp., Phytophthora spp., Sclerotium spp. The culture of termite and whitegrub were already released and allowed to build up in the microplot. The groundnut seeds were also inoculated with Aspergillus niger before seed treatment. 200 seeds were sown per plot and 4 replications per treatment. The observation on seed germination and plant stand were recorded after 20 days of sowing and presented in below table after calculating synergism by using Colby’s formula.
Table 9: Seed treatment in Groundnut / Peanut, Arachis hypogaea.
Treatment details % Seed germination
Obs.Value Cal.Value Ratio
Flub 6+Trifloxy 2.5+Hexa 1 gai/10 kg seed 96.8 87.71 1.10
Flub 6+Trifloxy 2.5 gai/10 kg seed 71.2 74.60 0.95
Flub 6+Hexa 1gai/10 kg seed 67.6 71.54 0.94
Trifloxy 2.5+ Hexa 1 gai/10 kg seed 78.4 79.09 0.99
Flub 6 gai/10 kg seed 41.2
Trifloxy 2.5 gai/10 kg seed 56.8
Hexa 1gai/10 kg seed 51.6
Untreated Check (UTC) 40.4
g.a.i. Gram Active Ingredient, kg Kilogram, Flub Flubendiamide, Trifloxy Trifloxystrobin, Hexa Hexaconazole.
The seed treatment observations on Groundnut trials shows that plant germination and plant stand was observed highest in treatment of Flubendiamide + Trifloxystrobin + Hexaconazole compared to other prior art treatment. The groundnut seed get protection against soil insects termite and white grub and also against soil and seed born fungi Rhizoctonia spp., Phytophthora spp., Sclerotium spp. and Agpergillus spp.
,CLAIMS:[CLAIM 1]. A synergistic pesticidal composition comprising component (A) Flubendiamide component (B) at least one Strobilurin fungicides from Trifloxystrobin, Azoxystrobin and component (C) at least one more triazole fungicide selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole, Tricyclazole and one or more inactive excipients..

[CLAIM 2]. The synergistic pesticidal composition as claimed in claim 1 wherein Flubendiamide is in the ratio of 1 to 40%, Strobilurin fungicides is in the ration 1 to 30% and triazole fungicides is in ratio of 1 to 30%.

[CLAIM 3]. The synergistic pesticidal composition as claimed in claim 1 or 2, wherein inactive excipients are selected from the group consisting of dispersant, anti-freezing agent, anti-foam agent, wetting agent, suspension aid, anti-microbial agent, thickener, quick coating agent or sticking agents and buffering agent.

[CLAIM 4]. The synergistic pesticidal composition as claimed in claims 1-3, wherein the composition are selected from formulations comprising of water-dispersible granules (WDG) or suspension concentrate (SC).

[CLAIM 5]. A suspension concentrate (SC) of synergistic pesticidal composition as claimed in claim 1 comprising
a) 1 to 40% Flubendiamide;
b) 1% to 30% Strobilurin fungicides selected from Trifloxystrobin, Azoxystrobin
c) 1% to 30% triazole fungicide selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole and Tricyclazole and
d) 0.2 to 20% wetting agent selected from Trisiloxane ethoxylate, sodium lauryl sulphate; sodium dioctylsulphosuccinate; alkyl phenol ethoxylates; and aliphatic alcohol ethoxylates or mixtures thereof.

[CLAIM 6]. The suspension concentrate (SC) of synergistic pesticidal composition according to claim 5, wherein wetting agent is Trisiloxane ethoxylate.

[CLAIM 7]. A water-dispersible granule (WG) of synergistic pesticidal composition as claimed in claim 1 comprising
a) 1 to 40% Flubendiamide;
b) 1% to 30% Strobilurin fungicides selected from Trifloxystrobin, Azoxystrobin
c) 1% to 30% triazole fungicide selected from Difenoconazole, Epoxiconazole, Flutriafol, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole and Tricyclazole and
d) 0.1 to 10% Polyacrylate co-polymer.

[CLAIM 8]. The water-dispersible granule (WG) of synergistic pesticidal composition according to claim 7, wherein acrylate polymers are poly-methacrylate, poly ethyl methacrylate, poly-methylmethacrylate, acrylate copoylmers and styrene-acrylic copolymers , poly-styrene-co maleic anhydride.

[CLAIM 9]. The synergistic pesticidal composition as claimed in any of the preceding claims, wherein the said synergistic composition is used for crop selected from Paddy/ Rice, Wheat, Maize, Chilli, Tomato, Brinjal, Cumin, Onion and Cole crop.

[CLAIM 10]. The synergistic pesticidal composition as claimed in in any preceding claims, wherein the composition control inects-pests selected from Stem borer (Scirpophaga incertulas Walker), Leaf folder (Cnaphalocrocis medinalis), Pink Stem borer (Sesamia inferens), Spodoptera litura Shoot borer (Chilo partellus), Corn worm (Helicoverpa armigera), Fruit borer complex (Helicovera armigera, Spodoptera litura, Spodoptera exigua), Fruit borer (Helicoverpa armigera), Fruit & Shoot borer (Leucinoides orbonalis), Spodoptera litura, Helicoverpa armigera Spodoptera litura & Helicoverpa, armigera Plutella xylostella and Spodoptera litura and fungal disease selected from Sheath Blight (Rhizoctonia solani), Blast (Pyricularia oryza), Helminothosporium leaf spot, False smut, (Ustilaginoidea virens), Grains discoloration (Curvularia spp., Helminthosporium spp., Drechslera spp.), Rust (Puccinia striiformis), Powdery mildew (Blumeria graminis), Grain discoloration, Head blight (Fusarium spp.), Septoria leaf spot, Leaf blight (Helminthosporium spp., Drechshlera spp., Bipolaris turcicum), Die back (Colletotrichus capsici), Fruit rot (Phytophthora capsici), Leaf spot (Cercospora spp., and Alternaria spp.), Early Blight (Alternaria solani), Late blight (Phytophthora spp.), Leaf blight (Phoma spp.,), Leaf spot (Alternaria spp., Cercospora spp.), Blight (Alternaria spp.), Powdery mildew (Erysiphe spp.), Purple blotch (Alternaria porri), Stemphylium Leaf blight, Black mould (Aspergillus spp.) and Alternaria leaf spot, Downy mildew (Peronospora spp.).

[CLAIM 11]. The synergistic pesticidal composition according to any preceding claims, wherein composition is to be applied at before or after insect-pest and fungal diseases infection.