FIELD OF INVENTION:
The present invention relates to a novel method for the synthesis of magnetic iron
. , oxide nanoparticles using cinnamon leaf extract. The present invention more
particularly relates to the novel green synthesis of magnetic iron oxide using
cinnamon leaf extract and its uses thereof.
DESCRIPTION OF THE RELATED ART:
Nanoparticles are of great scientific interest as they are, in effect, a bridge
between bulk materials and atomic or molecular structures. A bulk material
should have constant physical properties regardless of its size, but at the nnnoscale
size-dependent properties are often observed.. Thus, the properties of
materials change as their size approaches the nano scale. and as the percentage of
atoms at the surfae of a material becomes significant.
The presence of titanium dioxide nanoparticles imparts the self-cleaning effect,
and the size being nano-range, the particles cannot be observed. Zinc oxide
particles have been found to have superior UV blocking properties compared to
its bulk substitute. This is one of the reasons why it is often used in the
preparation of sunscreen lotions and is completely photostable. .
Magnetic nanoparticles have been attracting much interest as a remarkable
material in the field of'biological and medical applications such as drug delivery,
magneto caloric refrigerators, magnetic fluids and biosensors. Iron nanoparticles
(Fe20flFe304) has been especially interesting due to its non-toxicity, thermal and
chemical stability and favorable hysteric properties and is being seen as a valuable
material for mollifying bioIogicalIy active compounds, bio-tagging of drug
1
molecules, hysteretic heating of maligned cells etc. It has got a chemically active
surface where a variety of bonds can be fomied allowing for coating to which a
variety ofbio-active molecules may be attached for different applications.
-
9 "Cinnamon zeylanicum bark extract and powder mediated green synthesis of
A
U . ' nano-crystalline silver particles and its bactericidal activity" talks about the n I - exploitation of various plant materials for the biosynthesis of nanoparticles which
is considered a green technology as it does not involve any harmful chemicals.
Thus Chamon zeylanicum bark extract and powder are a good bio-resourcehiomaterial
for the synthesis of Ag'nanoparticles with antimicrobial activity. (Sathishkumar h4, et al.,
. .
Environmental Biotechnolog National Research Laboratory, Republic of Korea,
PubMed, 2009)
"Synthesis and surf'ace engineering of iron oxide nanoparticfes for biomedical
applications" talks about the Super-paramagnetic iron oxide nanoparticles (SPION) with
appropriate surface chemistry which have been widely used experimentally For numerous
in vivo applications such as magnetic resonance imaging contrast enhanncement, tissue
repair, irnrnunoassay, detoxification of biological fluids, hyperthemia, drug delivery and
in cell separation, etc. (Gupta AK, Gupla M, Crusade Laborato~ies Limited, Soushem
General Hospital, Glasgow, Scofla~ldU, K,B iomaterials, PubMed, 2005)
"An Effective Strategy for tksynthesis of Biocompatible Gold Nanoparticles Using
Cinnamon Phytochemicals for Phantom CT Imaging and Photoacoustic Detection of
Cancerous Cells" talks about non-toxic Cin-AuNPs that can serve as excellent CTJ
photoacoustic contrast-enhancement agents and may provide a novel approach towards
tumor detect ion through nanopharrnaceuticals. wripen Ckrmda, er al., Pharmaceuiicnl
ResearchI Volume 28, Issue 2,201 1)
Thus in the view of above prior art, there are many methods for the synthesis of
metal nanoparticles from plant extracts but the synthesis af magnetic iron oxide
using cinnamon plant extract is not found anywhere.
Therefore, the present invention provides the: novel green synthesis of magnetic
iron oxide (Fe30.d Fez03) using Cinnamon leaf extract and its uses thereof. The
present invention relates to a novel, cost-effective and user-friendly method for
the synthesis of maghetic iron oxide (Fe304/ FezOJ) using Cinnamon leaf extract.
OBJECTS OF THE INVENTION:
The principal object of the present invention is td provide a novel method for the
synthesis of magnetic iron oxide nanoparticles using cinnamon plant extract and
its'uses thereof.
Another object of the present invention is to provide a novel method for the
synthesis of magnetic iron oxide nanoparticles using cinnamon leaf with different
morphology and dimensions of nanoparticles.
Still another object of the present invention is to provide novel method for the
synthesis of magnetic iron oxide nanopartioles using extract of any aerial part of
cinnamon plants.
Yet another object of the present invention is to provide novel magnetic iron
. oxide narroparticles that 'are tthermo stable as well as photo stable in nature
Still another object i f the present invention is to provide novel magnetic iron
oxide nanoparticles that can be used for the removal of contaminants from any
soil or water reservoir site. .
Yet another object of the present invention is to'provide novel magnetic iron
oxide nanoparticles which have wide medical and industrial applications.
Still another object of the present invention is to provide a nbvel method for the '
. .
synthesis of magnetic ikn oxide nanopafiicles using cinnamon leaf that is user- . .
friendly as well as cost-effective in nature.
S-RY OF THE LnVENTION:
' The present invention relates to a novel method for the synthesis of magnetic iron
oxide nanoparticles using cinnamon leaf extract. The present method comprises
cinnamon leaf extract and FeC13, FeCI2 as precursors. The method involves the
dropwise addition of aqueous extract of Ci~~nimornuZme yIanicum to the Ferric
chloride and ferrous chloride solution. The solution is characterized using
Dynamic Light Scattering analysis (DLS) and UV -Visible spectroscopy (WVis).
In a preferred embodiment of the present invention, the synthesis of Iron
oxide .nanoparticle from plant extract of Cinnamornum Zeylanicum using ferric
chloride and femus chloride soIution,~ps rovided.
In another embodiment of the present invention the use of ferric chloride and
femus chloride solution for preparing iron nanoparticles from plant extract is
provided.
00
0 4 . In another embodiment of the present invention, the characterization of '
2 00 nanoparticles formed is done by using Dynamic Light Scattering analysis @LS)
0
7 and UV -Visible spectpescopy (UV-Vis).
I0
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BRIEF DESCRIPTION OF THE DRAWINGS:
It is to be noted, however, that the appended drawings illustrate only typical
embodiments of this invention and are therefore not to be considered for limiting
of its scope, for the invention may admit to other.equaIly effective embodiments.
Fig 1: Illustrates the formation and stability of synthesized sample using a W-,
Vis spectrophotdmeter (UV-VIS, VSI-SO 1 spectrophotometer) after a fixed
interval of time.
Fig 2: Illustrates the DLS results of Fe304 nanoparticles synthesized using
aqueous extract of Cinnamomum Zeylanicum.
DESCRIPTION OF THE PREFEWXED EMBODIMENTS:
Accordingly the present invention provides a method for the synthesis of
magnetic iron oxide nanoparticles using cinnamon leaf extract. The present
invention relates to a novel method for the synthesis of magnetic iron oxide
nanoparticles using cinnamon leaf extract. The present method comprises
cinnamon leaf extract and FeCI3, FeC12 as .precursors. The method involves the
drop wise addition of aqueous extract of Cinnamomim Zeylanicetm to the Ferric
chloride and ferrous chloride solution. Then the solution is kept under observance
for next 5 hours and a color change of the mixture is recorded periodically. The
solution is characterized using Dynamic Light Scattering analysis (DLS) and W
-Visible spectroscopy (UV-Vis). The average particle .size (radius) formed is
90nm and almost same size nanoparticles with uniform distribution are formed.
The stability of the'particles is more than 15 hours.
In the present invention, the leaves extract of cinnamomum zeylanicum is
prepared by shade drying and' I Ogrn leaves are boiled with DDW (1000rnl) in 'a
flask while being continuously stirred for 45 minutes. The extract is cooled to
room temperature, filtered and is stored in a fridge. The iron nanoparticles are
prepared by usingo. lM ferric chloride and 0.2M ferrous chloride is prepared in SO
-
U1 a ml of distilled water and i; is subjected to constant stirring for 10 minutes.
I - , Aqueous extract of Cinnamomum Zeylanicum of 10 ml is added drop-wise over a
period of 5 minutes and left to stir for another 10 minutes.
Then pH is maintained at 12 by periodic addition of Sodium hydroxide solution.
The final reaction mixture is centrifuged at an rpm of 4500 and the supernatant is
. used for further analysis.
:6-
In an embodiment of the invention, the characterization of solution using
Dynamic Light Scattering analysis @LS) and UV -Visible spectroscopy (UVVis)
is done.
Fig 1: Illustrates the formation and stability of synthesized sample was observed
using a UV-Vis spectrophotometer (W-VIS, VSTJOI spectroph~t~rnetearf)t er a
fixed interval of time. Figure1 shows the W-visible spectra of synthesized
"Fe304 nanoparticles. Sample displays a peak around 445nm which corresponds
to the characteristic peak of nanoparticles due to surface plasma resonance. The
absorption spectrum clearly indicates that the particles show stability.
Fig 2: Illustrates the DL§ results of Fe304 nanoparticles synthesized using
aqueous extract of Cinnamomum Zeylanicum. The average particle size (radius)
was 90nm.The observation also reveals that almost same size nanoparticles with
uniform distribution were formed. The stability of the particles was more than 15
hours.
, .
The invention is described in detail with reference to the examples given
below. The examples .are provided just to illustrate the invention and
therefore, should not be construed to limit the scope of the inventipn.
A
CU -. al Example 1:
Ca
e
Ql preparation of Extract
I
.C-r
F Cinnammum Zeylanicum leaves were shade dried and lOgm leaves were boiled
V
CV with DDW (1000ml) in a flask while being continuously stirred for 45 minutes.
E The extract was cooled to room temperature, filtered and stored in a fridge before . 8
LL use.
2
7 The synthesis of Iron Oxide Nanopirticle from plant extract is as follows: 0.1M
0
c?l ferric Chloride and 0.2M Ferrous Chloride was prepared in 50 ml of distilled
4 .
LU ,water and it was subjected to constant stirring for 10 minutes. Aqueous extract of '
a I Cinnamomum Zeylanicum of 10 ml was added drop-wise over a period of 5
7
00
' 0 4
minutes and left ,to stir for another 10 minutes. Then Sodium hydroxide solution
was added drop wise till the pH of the reaction mixture reached 12. The final
reaction mixture is centrifuged at an rprn of 4500 and the supernatarit is used for
. ,
, c'.
Example 2
Preparation of Iron Oxide nanoparticles of aqueous extract
0.1M Ferric Chloride and O.2M ~ermus Chloride.was prepared in 50 ml of
distilled water and it was subjected to constant stirring for 10 minutes. Aqueous
extract of Cinnnmomum Zeylanicurn of 10 ml was added drop-wise over a period
of 5 minutes and left to stir for another 10 minutes. Then pH was maintained at 12 .- '
by periodic addition af Sodium hydroxide solution. The final reaction mixtbre as
, centrifuged at an rpm of 4500 and the supernatant was used for further analysis.
Example 3 ,
Dynamic Light Scattering @LS)
When a beam of light passed through a colloidal dispersion, the particles
scattered some of the light in all directions. When the particles are small enough
to undergo random thermal (Brownian) motion and the distance between them is
therefore constantly varying. constructive and destructive interference of light
scattered by neighboring particles within the illuminated zone gave rise to the
intensity fluctuation at the detector plane. Analysis sf the time dependence of the
intensity fluctuation can therefore yield the diffusion coefficient of the particles
from which, via the Stokes Einstein equation, knowing the viscosity of the
medium, the hydrodynamic radius or diameter of the particles can be calculated.
UV - Vis Spectrophotometer
\ When electromagnetic radiation passed through a transparent medium, some
portion of radiation was absorbed and as a result of absorbed energy, atoms
passed from low energy state (ground) to high energy state (excited). UV- Visible
.spectrophotometer is a technique to measure the amount .of absorbed visible
radiation in a solution. From this technique we identify the organic compound. It
follows the Beer- Lambert Law.
Example 4
Characterization of Peso4 nanoparticles
Figure 1 shows the forination and stability of synthesized sample which was
observed using a UV-Vis spectrophotometer (UV-VI S, VSI-5 0 1
spectrophotometer) after a fixed interval of time. Figure 1 shows the UV-visible
spectra of synthesized Fe304 nanoparticles. Sample displays a peak around 445nm
which corresponds to the characteristic peak of nanoparticles due to surface ,
plasma resonance. The absorption spktrum clearly indicates that the' particles
show stability,
Example 4 . .
DLS resuIts of synthesized F404 nanoparticles
The particle size and particle distribution was analyzed by Dynamic light
scattering system. Fig 2 shows the DLS results of Fe304 nanoparticles synthesized
. ' using aqueous extract of Cinmmomum ZeyIanicum. The average particle s'ize ,
(radius) was 90nm.The obsehation also rev'eded that almost same size
nanoparticles with uniform distribution were formed. The stability of the particles
was more than 15 hours.
Numerous modifications and adaptations of the system of the present
invention will be apparent to those skilled in the art, and thus it is intended
by the appended claims :to cover all such modifications and adaptations .
which fa11 within the true spirit and scope of this invention. .
. .
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. ,
WE CLAIM -- I
1. A method for the synthesis of magnetic iron oxide.nanoparticles using
cinnamon leaf extract, comprising:
a. preparing aqueous extract from leaves of Cinnomomurn ZeyIanicum, ,
b. Adding solution of Ferric chloride and ferrous chloride solution to
step a;
c.. Mixing the solution in centrifuge;
d. Obtaining the iron nanopaAicles.
2. The'rnethod for the synthesis of magnetic iron oxide nanoparticles as
claimed in claim 1; wherein the aqueous extract is prepared form the
' leaves of Cinnamemum Zeylanicum.
3. The method for the synthesis of magnetic iron oxide ,nanoparticles as
claimed* in cIaim I; wherein ferric chloride used in the reaction is
. concentration is 0, I M.
4. The method. for the synthesis of magnetic iron oxide nanoparticles as
claimed in claim I; wherein the ferrous chloride concentration is 0.2M.
' 5. The method for the synthesis of magnetic imn oxide nanoparticles as
. . claimed in claim 1; wherein 'the aqueous extract of Cin~lamomum
Zeylarsicum is prepared by boiling 1 Ograms of shade dried leaves in DDW
for 45 m inultes.
6. The method fir the synthesis of magnetic iron oxide nanoparticles as
claimed in claiml; wherein ihe mixture is centrifuged at an rpm of 4500.
7. The method for the synthesis of magnetic iron oxide nanoparticles as
claimed in claim 1; wherein the nanoparticles are characterized by
dynamic Light Scattering analysis (DLS) and UV -Visible spectroscopy
('IN-Vis).
8. The method for the synthesis of magnetic iron oxide nanoparticles as
claimed in claim I; wherein the size of nanoparticles are obtained is of
90nm in size and show stability of 15 hours.